FastQCFastQC Report
Fri 27 May 2016
SRR939341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885068
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115920.4017929560065829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103110.357391922824696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69420.24061824539317617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103950.034.2943151
GTATCAA151900.029.8293631
GTACATG139100.026.1073151
TACATGG138350.025.6556592
ACATGGG143250.023.7511843
ATCAACG191000.023.7014943
TATCAAC191800.023.6273782
TCAACGC192700.023.5170274
CAACGCA194550.023.317795
CATGGGG65450.023.0556954
AACGCAG198800.022.7954276
GAGTACT107650.022.52631612-13
AGTACTT112550.021.25046212-13
GTACTTT115950.020.79104214-15
ACGCAGA222300.020.364317
CGCAGAG225250.020.0554718
ATGGGGA38550.019.3257335
ATATGCG1250.006068135618.981075
AGAGTAC203300.018.8370110-11
GCAGAGT237900.018.7500179
Produced by FastQC (version 0.11.5)