##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939341_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2885068 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30322196911823 33.0 31.0 34.0 31.0 34.0 2 32.508855597164434 34.0 31.0 34.0 31.0 34.0 3 32.59347855925753 34.0 31.0 34.0 31.0 34.0 4 36.12696650477562 37.0 35.0 37.0 35.0 37.0 5 36.070703012892594 37.0 35.0 37.0 35.0 37.0 6 36.10088497047557 37.0 35.0 37.0 35.0 37.0 7 36.040336657576184 37.0 35.0 37.0 35.0 37.0 8 36.01565162415583 37.0 35.0 37.0 35.0 37.0 9 37.71467639584232 39.0 37.0 39.0 35.0 39.0 10-11 37.6343567985226 39.0 37.0 39.0 35.0 39.0 12-13 37.555636470266904 39.0 37.0 39.0 35.0 39.0 14-15 38.846458211730194 40.0 38.0 41.0 35.0 41.0 16-17 38.84900477215788 40.0 38.0 41.0 35.0 41.0 18-19 38.95077862982779 40.0 38.0 41.0 35.0 41.0 20-21 38.93564744401172 40.0 38.0 41.0 35.0 41.0 22-23 38.85837370211031 40.0 38.0 41.0 35.0 41.0 24-25 38.78874622712532 40.0 38.0 41.0 34.0 41.0 26-27 38.63206725110119 40.0 38.0 41.0 34.0 41.0 28-29 38.45961966927643 40.0 38.0 41.0 34.0 41.0 30-31 38.255351866923064 40.0 38.0 41.0 34.0 41.0 32-33 38.199148165658485 40.0 38.0 41.0 34.0 41.0 34-35 38.10255078909752 40.0 38.0 41.0 33.0 41.0 36-37 37.946873522565156 40.0 37.0 41.0 33.0 41.0 38-39 37.81406902714251 40.0 37.0 41.0 33.0 41.0 40-41 37.6250244361658 40.0 37.0 41.0 33.0 41.0 42-43 37.43027512696408 40.0 37.0 41.0 32.5 41.0 44-45 37.16733557060007 40.0 36.5 41.0 31.5 41.0 46-47 36.831168450795616 40.0 36.0 41.0 31.0 41.0 48-49 36.49761062824169 39.5 36.0 41.0 30.0 41.0 50-51 36.23658125215766 39.0 35.0 41.0 30.0 41.0 52-53 35.96648605856084 39.0 35.0 40.5 29.0 41.0 54-55 35.710368178497006 39.0 35.0 40.0 28.5 41.0 56-57 35.39356819319337 38.0 35.0 40.0 28.0 41.0 58-59 35.07342911848178 38.0 34.0 40.0 27.5 41.0 60-61 34.7304765433605 38.0 34.0 40.0 26.5 41.0 62-63 34.375678146927555 37.0 34.0 40.0 26.0 41.0 64-65 34.055134055765755 36.5 33.5 39.5 26.0 41.0 66-67 33.63660440585802 36.0 33.0 39.0 25.5 40.5 68-69 33.176287525978594 35.5 32.5 39.0 24.5 40.0 70-71 32.694100451011906 35.0 32.0 38.0 23.0 40.0 72-73 32.20724086919269 35.0 31.5 37.5 22.0 39.5 74-75 31.683539521425494 35.0 31.0 37.0 20.5 39.0 76-77 29.75312280334467 33.0 28.5 35.0 18.0 37.0 78-79 30.72251000669655 34.0 30.5 36.0 18.0 38.0 80-81 30.736858888594654 34.0 31.0 35.5 18.0 37.0 82-83 30.386747556730032 34.0 30.5 35.0 16.5 37.0 84-85 29.934287164115368 34.0 30.0 35.0 9.0 36.0 86-87 29.327548258827868 34.0 29.0 35.0 4.5 36.0 88-89 28.738402526387596 34.0 29.0 35.0 2.0 35.5 90-91 28.251164790569927 33.0 28.5 35.0 2.0 35.0 92-93 27.879673026770945 33.0 27.0 35.0 2.0 35.0 94-95 27.34399761114816 33.0 26.5 35.0 2.0 35.0 96-97 26.594292924811477 32.5 25.5 34.0 2.0 35.0 98-99 25.571913209671315 32.0 22.5 34.0 2.0 35.0 100-101 21.249841771493774 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20485070918073944 1101 2 0.21599031375620825 1101 3 0.25863475646550427 1101 4 0.07840509171285959 1101 5 0.15374701956277903 1101 6 0.20483213068384032 1101 7 0.14787218479604292 1101 8 0.1525435215965132 1101 9 0.2686410641645125 1101 10-11 0.26749561556732715 1101 12-13 0.30785344754354327 1101 14-15 0.3464472865663595 1101 16-17 0.2930813776915926 1101 18-19 0.22845046010611014 1101 20-21 0.23877752763835502 1101 22-23 0.3312869141171717 1101 24-25 0.29630977344034903 1101 26-27 0.32109270786066446 1101 28-29 0.3225202408999337 1101 30-31 0.37402523319911296 1101 32-33 0.4408479035490842 1101 34-35 0.47303782703748 1101 36-37 0.4245242215991354 1101 38-39 0.4349179905684437 1101 40-41 0.5763793628707035 1101 42-43 0.6041998337205783 1101 44-45 0.5741848302415207 1101 46-47 0.541916821709016 1101 48-49 0.5037355518773836 1101 50-51 0.4427493497471531 1101 52-53 0.4201744378885479 1101 54-55 0.4352050598989976 1101 56-57 0.4183255610869381 1101 58-59 0.4992118383396189 1101 60-61 0.39639552445210313 1101 62-63 0.3749827941783934 1101 64-65 0.4070450649729409 1101 66-67 0.34899377693291456 1101 68-69 0.5130213575748783 1101 70-71 0.348108678398539 1101 72-73 0.29787450657400427 1101 74-75 0.38980922915550664 1101 76-77 0.2609679920989656 1101 78-79 0.41324031967600305 1101 80-81 0.4902446712168924 1101 82-83 0.5264800616621201 1101 84-85 0.4751896781269309 1101 86-87 0.24790769552575398 1101 88-89 0.42821750225381905 1101 90-91 0.700099769557422 1101 92-93 0.6698633680913666 1101 94-95 0.5461549880662808 1101 96-97 0.415311350518742 1101 98-99 0.49281989132893145 1101 100-101 0.18416830567761622 1102 1 0.21201783627471826 1102 2 0.22996402037536967 1102 3 0.262340210939648 1102 4 0.16774289641046636 1102 5 0.23655010384147346 1102 6 0.19478537685983355 1102 7 0.20518736368527613 1102 8 0.2210203737592309 1102 9 0.30721267185310097 1102 10-11 0.2978753507438725 1102 12-13 0.2585439993848482 1102 14-15 0.2772838787426366 1102 16-17 0.29771783818704733 1102 18-19 0.3798671431635583 1102 20-21 0.330212584211786 1102 22-23 0.3830303926183163 1102 24-25 0.28197945600993535 1102 26-27 0.27479287370957906 1102 28-29 0.23706749595487508 1102 30-31 0.1846511509092963 1102 32-33 0.2717471570210037 1102 34-35 0.278223368649158 1102 36-37 0.3330119897220385 1102 38-39 0.35117617531022205 1102 40-41 0.38864460584738225 1102 42-43 0.41049197559597417 1102 44-45 0.34349536880777976 1102 46-47 0.3792548278497705 1102 48-49 0.4434257632262941 1102 50-51 0.3864870678063852 1102 52-53 0.43241708536245227 1102 54-55 0.38411500887268346 1102 56-57 0.32866093123424633 1102 58-59 0.3751376448698238 1102 60-61 0.3448714644755313 1102 62-63 0.32014027042266235 1102 64-65 0.24505001726539177 1102 66-67 0.30329715802827906 1102 68-69 0.2049287804872293 1102 70-71 0.196598924450349 1102 72-73 0.21325717129239052 1102 74-75 0.27980983526761705 1102 76-77 0.30093484608596555 1102 78-79 0.23390259222798093 1102 80-81 0.36193155548572875 1102 82-83 0.4186292460550902 1102 84-85 0.31082455381749696 1102 86-87 0.095109210568328 1102 88-89 0.19087833736933746 1102 90-91 0.3687927274260936 1102 92-93 0.22413818169703958 1102 94-95 0.3002096255072537 1102 96-97 0.11707463165314635 1102 98-99 -0.05504135000054333 1102 100-101 0.19263062600548508 1103 1 0.13870988996679046 1103 2 0.1932021025050119 1103 3 0.17982019622085232 1103 4 0.09308965683847958 1103 5 0.15261641859117248 1103 6 0.12396038007607046 1103 7 0.15080385769688576 1103 8 0.16632902270253425 1103 9 0.24108537018070564 1103 10-11 0.18498794526180973 1103 12-13 0.20105780120365324 1103 14-15 0.2632929172153169 1103 16-17 0.17743124607027028 1103 18-19 0.20672168128469792 1103 20-21 0.238724905603803 1103 22-23 0.30045165302650645 1103 24-25 0.23941622803702955 1103 26-27 0.21845162634611626 1103 28-29 0.24516172867130592 1103 30-31 0.26199961900061197 1103 32-33 0.24285311953346422 1103 34-35 0.3450581687289045 1103 36-37 0.29652977024309735 1103 38-39 0.27519223158942907 1103 40-41 0.2563845500049595 1103 42-43 0.25417741599789423 1103 44-45 0.3129102465517306 1103 46-47 0.3168414875627761 1103 48-49 0.31179171305653597 1103 50-51 0.32857918524846497 1103 52-53 0.15662216647130833 1103 54-55 0.24159998392602944 1103 56-57 0.3331145165535361 1103 58-59 0.2997738122296738 1103 60-61 0.2913948414696108 1103 62-63 0.19804227017774423 1103 64-65 0.38024801145361664 1103 66-67 0.17606268397840807 1103 68-69 0.23548538765437144 1103 70-71 0.32118989502587425 1103 72-73 0.25904731998495123 1103 74-75 0.1588573752996325 1103 76-77 0.1389051847936429 1103 78-79 0.18708788975972368 1103 80-81 0.42089479106265415 1103 82-83 0.34635020078447454 1103 84-85 0.4386560026743247 1103 86-87 0.40359944197674835 1103 88-89 0.49907848116790987 1103 90-91 0.6494899574541471 1103 92-93 0.4753115370800778 1103 94-95 0.28885818860466017 1103 96-97 0.19799942240618407 1103 98-99 -0.1561180561791531 1103 100-101 -0.24097482625634825 1104 1 0.07038724069879976 1104 2 0.09190561398848729 1104 3 0.12727997413677627 1104 4 0.06412077370352165 1104 5 0.11388316472295656 1104 6 0.028128591369622313 1104 7 0.04103962954456364 1104 8 0.147434053528535 1104 9 0.18750188333522289 1104 10-11 0.14858132722475403 1104 12-13 0.100600900571159 1104 14-15 0.08116372747429779 1104 16-17 0.1532957143288982 1104 18-19 0.12995909566483732 1104 20-21 0.062079450228793576 1104 22-23 0.085099036095869 1104 24-25 0.15300319385752204 1104 26-27 0.16532896110906137 1104 28-29 0.1487184102994661 1104 30-31 0.11481473714318469 1104 32-33 0.03502350090897011 1104 34-35 0.16065310197250682 1104 36-37 0.1997682824964926 1104 38-39 0.16047424243698316 1104 40-41 0.26212350425929287 1104 42-43 0.22508242001867274 1104 44-45 0.24376604588901074 1104 46-47 0.23665988221542733 1104 48-49 0.21930715457268946 1104 50-51 0.2229234982649686 1104 52-53 0.4176128046065699 1104 54-55 0.43234762135980276 1104 56-57 0.10004600355870963 1104 58-59 0.18719047366837316 1104 60-61 0.2573480806234798 1104 62-63 0.32602256834300647 1104 64-65 0.24379448428624073 1104 66-67 0.22651516460165766 1104 68-69 0.2470711586465697 1104 70-71 0.11516620662796839 1104 72-73 0.1590898091060282 1104 74-75 0.2666360925274027 1104 76-77 0.34053759401235695 1104 78-79 0.44500761954031987 1104 80-81 0.4611571542562416 1104 82-83 0.32905491850272384 1104 84-85 0.394803665202339 1104 86-87 0.4028633678574671 1104 88-89 0.5167521019824477 1104 90-91 0.555217861685378 1104 92-93 0.5078974848726396 1104 94-95 0.32530316091544265 1104 96-97 0.27340043130893577 1104 98-99 0.16405778889809852 1104 100-101 0.003982370944502378 1105 1 0.04572615746866404 1105 2 0.028515685899670018 1105 3 -0.043094137836114044 1105 4 0.05217450070171736 1105 5 0.050438379913380516 1105 6 0.06943515896831087 1105 7 0.08079608742981037 1105 8 0.03558663125127026 1105 9 0.04490754063822777 1105 10-11 0.08260389995064088 1105 12-13 0.06702722962459262 1105 14-15 0.0548156642967399 1105 16-17 0.0523734696570628 1105 18-19 -0.020126176663474382 1105 20-21 -0.027943415790460335 1105 22-23 -0.0031341973004614943 1105 24-25 0.025093615113689793 1105 26-27 0.15344732127331184 1105 28-29 0.1763537054394888 1105 30-31 0.12094945655920242 1105 32-33 0.06532532150033177 1105 34-35 0.10275536359018389 1105 36-37 0.02468979936629978 1105 38-39 0.0659422625981918 1105 40-41 0.16565959020510235 1105 42-43 0.13861763694713147 1105 44-45 0.009716316370663947 1105 46-47 0.018742102120263837 1105 48-49 0.015411784262667538 1105 50-51 0.085397427744212 1105 52-53 0.09572415831836878 1105 54-55 0.0994976789322024 1105 56-57 0.09549180946367386 1105 58-59 0.025425608040180236 1105 60-61 4.0897701488518123E-4 1105 62-63 0.08324491729693051 1105 64-65 0.09070349816879286 1105 66-67 0.21309889442416363 1105 68-69 0.01785832543271937 1105 70-71 -0.0024555644294110834 1105 72-73 0.06518868155600188 1105 74-75 0.16282246416267654 1105 76-77 0.2854250562644154 1105 78-79 0.05582335527214255 1105 80-81 0.038378851447141926 1105 82-83 0.1843996468261757 1105 84-85 0.1857322102500305 1105 86-87 0.18390765515460927 1105 88-89 0.23285227795846097 1105 90-91 0.34166119089087843 1105 92-93 0.38242305570841495 1105 94-95 0.3510945098476377 1105 96-97 0.24947285304305922 1105 98-99 0.14381918931957216 1105 100-101 0.15010655352504898 1106 1 0.08541688953543058 1106 2 0.10434869406690694 1106 3 0.11403337339183395 1106 4 0.09714561921607867 1106 5 0.0528939668887034 1106 6 0.11000746683919971 1106 7 0.09130530012367188 1106 8 0.08568915573200542 1106 9 0.0955728226932564 1106 10-11 0.14787995689590616 1106 12-13 0.12754633980433283 1106 14-15 0.2128377709273792 1106 16-17 0.08390149638338329 1106 18-19 0.16498816002619066 1106 20-21 0.19019503552764405 1106 22-23 0.27623898870024277 1106 24-25 0.2542145188629661 1106 26-27 0.20074324781013075 1106 28-29 0.223590622750919 1106 30-31 0.2062917592947997 1106 32-33 0.23221662482303884 1106 34-35 0.2106406154552971 1106 36-37 0.22202383899666245 1106 38-39 0.2544692239197488 1106 40-41 0.3795235324203716 1106 42-43 0.1592720317676921 1106 44-45 0.3503051286879071 1106 46-47 0.32388942956588807 1106 48-49 0.23699045398873864 1106 50-51 0.32137337685351497 1106 52-53 0.4768109178141273 1106 54-55 0.44600308912598763 1106 56-57 0.4952309899829359 1106 58-59 0.47462345826479435 1106 60-61 0.5362082741379339 1106 62-63 0.601815528604007 1106 64-65 0.4050067125716552 1106 66-67 0.4917081344267942 1106 68-69 0.5026147073678899 1106 70-71 0.4502447521346795 1106 72-73 0.47794046504668586 1106 74-75 0.5306844206624426 1106 76-77 0.42116104316034964 1106 78-79 0.46668056046049955 1106 80-81 0.5526617397304356 1106 82-83 0.6895839084763189 1106 84-85 0.7513066168024736 1106 86-87 0.7869463128689738 1106 88-89 0.994736645268155 1106 90-91 1.0673551549272489 1106 92-93 1.0779601964128354 1106 94-95 1.3791130098551498 1106 96-97 1.2518277810323752 1106 98-99 1.1460410064876143 1106 100-101 1.069914635573653 1107 1 0.052511232122668616 1107 2 0.047372122528365423 1107 3 0.0186442837476406 1107 4 0.04986735308973067 1107 5 0.020667943121040366 1107 6 0.08149793479821454 1107 7 0.03859367058157659 1107 8 0.046217288636817955 1107 9 0.08559374532989494 1107 10-11 0.060688901773936266 1107 12-13 0.02185855228483291 1107 14-15 0.05538146598507865 1107 16-17 0.08236083287220453 1107 18-19 0.09627265279245734 1107 20-21 0.1017713878343045 1107 22-23 0.12242036663954536 1107 24-25 0.1309540882405713 1107 26-27 0.10693175560293611 1107 28-29 0.05159550642881072 1107 30-31 0.1088668568361868 1107 32-33 0.10488623615708548 1107 34-35 0.18174852699866761 1107 36-37 0.16784924306492854 1107 38-39 0.1704329930972861 1107 40-41 0.23023320730547425 1107 42-43 0.19439878924816867 1107 44-45 0.054620085890256576 1107 46-47 0.017606079404352215 1107 48-49 0.20285868905848048 1107 50-51 0.29117587793497535 1107 52-53 0.2214556220944317 1107 54-55 0.3188251918695002 1107 56-57 0.31725109537926954 1107 58-59 0.19446672254197495 1107 60-61 0.3606726272173688 1107 62-63 0.2989483195315543 1107 64-65 0.3977000950250513 1107 66-67 0.4907959035163856 1107 68-69 0.5908421976224005 1107 70-71 0.41095051375299363 1107 72-73 0.4320459858903902 1107 74-75 0.3421375322407023 1107 76-77 0.5020229327592887 1107 78-79 0.6790279671430426 1107 80-81 0.6239192027921874 1107 82-83 0.6564186636216824 1107 84-85 0.5459562126662689 1107 86-87 0.6922230838507168 1107 88-89 1.002558237854096 1107 90-91 0.8449806197203245 1107 92-93 0.802804240131735 1107 94-95 1.0024456407439182 1107 96-97 1.0402540103947686 1107 98-99 0.9830235516872037 1107 100-101 0.7788780176243577 1108 1 0.027326776410362186 1108 2 0.035267382657913515 1108 3 0.013070357823110612 1108 4 0.03248317803620182 1108 5 0.06105573458234659 1108 6 0.042579759718677224 1108 7 0.08011639223875733 1108 8 0.07781303595879763 1108 9 0.09561612473469694 1108 10-11 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-0.3464754600563431 2316 84-85 -0.3566741059195948 2316 86-87 -0.46626968914212696 2316 88-89 -0.8964546330764698 2316 90-91 -1.0680686317525705 2316 92-93 -0.9008963057867518 2316 94-95 -0.581637472711261 2316 96-97 -0.48014245976474257 2316 98-99 -0.32547366204254047 2316 100-101 0.2478614180329437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 18.0 5 94.0 6 150.0 7 366.0 8 797.0 9 1540.0 10 2663.0 11 3983.0 12 5906.0 13 8274.0 14 10470.0 15 13919.0 16 18655.0 17 26217.0 18 25389.0 19 16994.0 20 15378.0 21 16606.0 22 17900.0 23 19634.0 24 22792.0 25 26918.0 26 32002.0 27 39078.0 28 47721.0 29 57762.0 30 70320.0 31 85573.0 32 107365.0 33 139014.0 34 189689.0 35 274343.0 36 430445.0 37 662924.0 38 466909.0 39 27250.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00488810817594 19.30800991318489 14.698149499421708 22.98895247921746 2 15.646078359331565 20.687553984862745 39.469433649397516 24.19693400640817 3 19.221002763193106 24.534430384309832 30.04362462167269 26.20094223082437 4 13.140071568503759 16.835339756289976 36.19422488482074 33.83036379038553 5 13.904351647864106 37.48237476551679 33.72076498716841 14.892508599450688 6 32.3781276559166 35.715622647369145 17.130064178729928 14.776185517984326 7 28.89009895087395 32.96594742307634 20.108919443146576 18.035034182903143 8 26.729248669355453 35.30786796013127 19.379196608190863 18.58368676232241 9 26.781413817629257 16.749518555541844 20.056996923469395 36.412070703359504 10-11 25.429833889530506 26.6880191385437 26.593411316475034 21.288735655450754 12-13 26.02111977949913 23.950995262503344 27.55401259173094 22.473872366266583 14-15 22.834429511009436 25.263507248637364 26.008925399259976 25.893137841093232 16-17 22.385918964480084 28.70334537416854 26.58152294757428 22.329212713777107 18-19 22.262092262643378 26.82137821361576 29.291354657845154 21.62517486589571 20-21 22.997343563479266 26.02318212257042 29.250783690367086 21.728690623583223 22-23 22.62948394977172 25.95365169902408 29.242603640538107 22.17426071066609 24-25 22.488620718818414 26.172849305458314 29.283833171349862 22.054696804373414 26-27 22.306734057383782 26.48693305456444 29.158483722729393 22.04784916532239 28-29 22.077503892455912 26.485684219574722 29.412512980629916 22.024298907339446 30-31 22.198367594801923 26.32520273352309 29.2774555053815 22.198974166293482 32-33 21.993329100042008 26.48221809676583 29.182501071031947 22.34195173216021 34-35 22.65176996794481 26.377822160231595 28.838928203023613 22.131479668799983 36-37 22.62005018986285 26.238268219216593 28.890645963536006 22.251035627384553 38-39 22.34915892256126 26.485672394011768 29.08873167671292 22.076437006714063 40-41 22.3866602749346 26.314542542280233 29.14056160586376 22.158235576921413 42-43 22.294048151139933 26.387340443175994 28.759178963775987 22.559432441908083 44-45 22.378060859216465 26.703692207600216 28.492561010081157 22.425685923102158 46-47 22.456307272372783 26.86680097918156 28.106566148913586 22.570325599532072 48-49 22.67688852756365 26.886612524494737 27.95728405507202 22.47921489286959 50-51 22.587222411125982 27.03800293026124 27.829197727470458 22.545576931142328 52-53 22.78974650484478 27.002441886058026 27.68391700013691 22.523894608960283 54-55 22.30878093687913 27.020454977144386 27.812620014502258 22.858144071474225 56-57 22.518306929033777 27.393973362254926 27.59455973163644 22.493159977074853 58-59 22.56411980619128 26.922407014604282 27.813305294142012 22.700167885062424 60-61 22.61012641730744 26.874284240678143 28.083640202866643 22.431949139147772 62-63 22.482846828548006 26.908772337250674 28.088255428443677 22.520125405757643 64-65 22.306764852733895 26.723404815798872 28.049301463241832 22.920528868225396 66-67 22.27746288845697 27.116127002888163 27.79183394409634 22.81457616455853 68-69 22.288239549368342 27.011175324096875 27.919679502755095 22.780905623779688 70-71 22.20360144024335 26.947181140964442 27.925684940528267 22.923532478263944 72-73 22.378553989022095 26.929867857534035 27.644686364411513 23.046891789032355 74-75 22.266896701723617 27.18156507603375 27.409618665767788 23.14191955647485 76-77 22.312581415799578 26.898294562167397 27.678722069287502 23.11040195274552 78-79 22.25281081432378 27.08100777712788 27.584151123242563 23.082030285305777 80-81 22.114406434517072 27.387095264751398 27.54236039742605 22.95613790330547 82-83 22.319664562499046 27.176673132141076 27.354017305657962 23.149644999701913 84-85 22.17945989487943 26.991755480286773 27.342665753458846 23.486118871374956 86-87 22.12021574266331 26.9364305859362 27.60667075648727 23.336682914913226 88-89 22.01492264683011 26.845925150174573 27.60938432339244 23.529767879602876 90-91 21.938227534836514 27.006145268872896 27.67167997330562 23.383947222984975 92-93 21.967939750466886 26.741137470589948 27.636800935021288 23.654121843921878 94-95 21.762641296496305 26.59661748007326 27.757907265963922 23.882833957466516 96-97 21.842084831276072 26.46006957201702 27.772309006234863 23.925536590472042 98-99 21.637702569022686 26.497071470808642 27.525384167987838 24.339841792180838 100-101 21.668755015069035 25.977667670113743 28.265423016198998 24.088154298618225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1087.0 1 749.0 2 559.5 3 743.0 4 655.5 5 643.5 6 1120.0 7 2337.5 8 3727.5 9 4317.0 10 3817.0 11 3299.0 12 3446.0 13 3760.0 14 4066.0 15 4258.0 16 4436.0 17 4642.5 18 4901.0 19 5244.0 20 5721.5 21 6487.0 22 7556.0 23 9086.0 24 10976.0 25 13398.0 26 16658.5 27 20318.5 28 23361.0 29 26834.5 30 31184.5 31 35493.5 32 40889.0 33 45455.5 34 49875.5 35 54411.0 36 59764.5 37 66596.0 38 71430.5 39 74436.0 40 77072.0 41 83048.5 42 90293.0 43 95204.5 44 99547.0 45 104804.5 46 105530.5 47 107416.0 48 119566.0 49 121599.5 50 113562.5 51 109052.0 52 104183.5 53 103106.0 54 103324.5 55 96689.5 56 85456.0 57 75928.0 58 67457.0 59 58221.5 60 49557.5 61 41102.0 62 34048.5 63 26738.5 64 19984.0 65 15485.5 66 11861.0 67 9929.0 68 7512.0 69 4761.5 70 3555.0 71 2784.0 72 2302.0 73 1966.5 74 1579.5 75 1324.0 76 931.5 77 525.5 78 310.0 79 203.5 80 130.5 81 87.5 82 49.0 83 29.0 84 20.0 85 10.0 86 5.5 87 3.5 88 6.0 89 3.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29444713261524513 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014904328078229007 16-17 8.492000881781644E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.932245617780932E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015250940359118053 36-37 0.007712123249781288 38-39 0.0027035757909345637 40-41 0.0031541717560903243 42-43 2.252979825778803E-4 44-45 0.0011264899128894015 46-47 1.9063675448897566E-4 48-49 3.292816668445943E-4 50-51 3.466122808890466E-5 52-53 1.03983684266714E-4 54-55 0.0 56-57 9.185225443559737E-4 58-59 0.0020276818432009226 60-61 6.932245617780933E-4 62-63 0.001143820526933854 64-65 5.1991842133357E-5 66-67 5.372490353780223E-4 68-69 0.001767722632534138 70-71 0.0 72-73 0.0 74-75 1.733061404445233E-5 76-77 0.2592139942628735 78-79 1.9063675448897566E-4 80-81 1.03983684266714E-4 82-83 0.0 84-85 0.0 86-87 0.10800438672502694 88-89 0.37825798213421663 90-91 0.2793001759403938 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.932245617780932E-5 100-101 4.505959651557606E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2885068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.32237661434823 #Duplication Level Percentage of deduplicated Percentage of total 1 70.13068281986234 32.48619901803064 2 15.471097037966656 14.333159672596377 3 5.89466383990397 8.191645152210354 4 2.730528413000704 5.059382620127887 5 1.612056128904035 3.7337135563280506 6 0.967095258222375 2.687889046399632 7 0.6220987002308679 2.0171963198373546 8 0.43453407412716194 1.61029208267884 9 0.33222454894401615 1.3850487609045012 >10 1.5903776117331065 13.250759403298096 >50 0.12336814118292543 3.9568948365345773 >100 0.08348613007556406 7.364472818930053 >500 0.006214861108075284 1.9839239100858514 >1k 0.0013478012041371241 0.9389282884944296 >5k 7.487784467428467E-5 0.24078463903687955 >10k+ 1.4975568934856933E-4 0.7597098745065936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11592 0.4017929560065829 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10311 0.357391922824696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6942 0.24061824539317617 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.4661228088904666E-4 14-15 0.0 0.0 0.0 6.932245617780932E-5 3.6394289493349896E-4 16-17 0.0 0.0 0.0 1.03983684266714E-4 3.986041230224037E-4 18-19 0.0 0.0 0.0 1.3864491235561865E-4 4.852571932446653E-4 20-21 0.0 0.0 0.0 1.3864491235561865E-4 5.372490353780223E-4 22-23 0.0 1.733061404445233E-5 0.0 2.2529798257788031E-4 5.545796494224746E-4 24-25 0.0 3.466122808890466E-5 0.0 4.67926579200213E-4 5.545796494224746E-4 26-27 0.0 3.466122808890466E-5 0.0 9.011919303115214E-4 5.545796494224746E-4 28-29 0.0 3.466122808890466E-5 0.0 0.00259959210666785 5.71910263466927E-4 30-31 0.0 3.466122808890466E-5 0.0 0.007365510968892241 5.892408775113793E-4 32-33 0.0 3.466122808890466E-5 0.0 0.015718866938318265 5.892408775113793E-4 34-35 0.0 3.466122808890466E-5 0.0 0.03018992966543596 5.892408775113793E-4 36-37 0.0 3.466122808890466E-5 0.0 0.05499003836304725 5.892408775113793E-4 38-39 0.0 3.466122808890466E-5 0.0 0.09360264645408704 5.892408775113793E-4 40-41 0.0 3.466122808890466E-5 0.0 0.13512679770459485 5.892408775113793E-4 42-43 0.0 3.466122808890466E-5 0.0 0.1778987531663032 6.065714915558316E-4 44-45 0.0 3.466122808890466E-5 0.0 0.23219556696757235 6.412327196447362E-4 46-47 0.0 3.466122808890466E-5 0.0 0.3000622515656477 6.585633336891886E-4 48-49 0.0 3.466122808890466E-5 0.0 0.37761674941457185 6.585633336891886E-4 50-51 0.0 3.466122808890466E-5 0.0 0.46399252981212225 6.932245617780933E-4 52-53 0.0 3.466122808890466E-5 0.0 0.5485312651209607 7.625470179559026E-4 54-55 0.0 3.466122808890466E-5 0.0 0.6445082056991378 7.625470179559026E-4 56-57 0.0 3.466122808890466E-5 0.0 0.7602247156739459 7.972082460448072E-4 58-59 0.0 3.466122808890466E-5 0.0 0.8946756194308072 7.972082460448072E-4 60-61 0.0 3.466122808890466E-5 0.0 1.0444467860029643 7.972082460448072E-4 62-63 0.0 3.466122808890466E-5 0.0 1.197926703980634 8.665307022226165E-4 64-65 0.0 3.466122808890466E-5 0.0 1.3563805081890616 8.665307022226165E-4 66-67 0.0 3.466122808890466E-5 0.0 1.5440363970623916 8.665307022226165E-4 68-69 0.0 3.466122808890466E-5 0.0 1.7597678806877344 8.665307022226165E-4 70-71 0.0 3.466122808890466E-5 0.0 2.003834918275756 8.838613162670688E-4 72-73 0.0 3.466122808890466E-5 0.0 2.25830032429045 0.0010225062286226875 74-75 0.0 3.466122808890466E-5 0.0 2.5178089389920792 0.0011611511409783063 76-77 0.0 3.466122808890466E-5 0.0 2.801389776601453 0.0011784817550227586 78-79 0.0 3.466122808890466E-5 0.0 3.1207756628266647 0.0011784817550227586 80-81 0.0 3.466122808890466E-5 0.0 3.483193463724252 0.001195812369067211 82-83 0.0 3.466122808890466E-5 0.0 3.8585572333130447 0.0012478042112005678 84-85 0.0 3.466122808890466E-5 0.0 4.23653792562255 0.0012478042112005678 86-87 0.0 3.466122808890466E-5 0.0 4.6427848494385575 0.0013344572814228295 88-89 0.0 3.466122808890466E-5 0.0 5.081249384763201 0.0014211103516450913 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10395 0.0 34.294315 1 GTATCAA 15190 0.0 29.829363 1 GTACATG 13910 0.0 26.107315 1 TACATGG 13835 0.0 25.655659 2 ACATGGG 14325 0.0 23.751184 3 ATCAACG 19100 0.0 23.701494 3 TATCAAC 19180 0.0 23.627378 2 TCAACGC 19270 0.0 23.517027 4 CAACGCA 19455 0.0 23.31779 5 CATGGGG 6545 0.0 23.055695 4 AACGCAG 19880 0.0 22.795427 6 GAGTACT 10765 0.0 22.526316 12-13 AGTACTT 11255 0.0 21.250462 12-13 GTACTTT 11595 0.0 20.791042 14-15 ACGCAGA 22230 0.0 20.36431 7 CGCAGAG 22525 0.0 20.055471 8 ATGGGGA 3855 0.0 19.325733 5 ATATGCG 125 0.0060681356 18.98107 5 AGAGTAC 20330 0.0 18.83701 10-11 GCAGAGT 23790 0.0 18.750017 9 >>END_MODULE