FastQCFastQC Report
Fri 27 May 2016
SRR939340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938708
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119860.4078663140400476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96680.32898811314359916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65520.22295512177460297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96100.033.8555531
GTATCAA146500.032.4533231
ATCAACG180600.026.179353
TCAACGC181500.025.997284
TATCAAC181800.025.9543782
CAACGCA183650.025.7187525
GTACATG136650.025.2689671
AACGCAG188800.025.0533566
TACATGG137750.024.477072
ACATGGG141000.022.9711673
ACGCAGA210400.022.3690877
CGCAGAG213300.021.9982978
GAGTACT102050.021.89845312-13
AGTACTT107600.020.9671512-13
CATGGGG69550.020.7962074
GCAGAGT224850.020.4677969
GTACTTT111450.019.96710414-15
GTATAGG7200.019.1305851
AGAGTAC197200.018.6507310-11
TACCTGG25000.018.5894932
Produced by FastQC (version 0.11.5)