##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2938708 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.920039350626194 33.0 31.0 34.0 30.0 34.0 2 31.998769527288864 33.0 31.0 34.0 30.0 34.0 3 32.04960036859735 34.0 31.0 34.0 30.0 34.0 4 35.54712853403605 37.0 35.0 37.0 33.0 37.0 5 35.58649107022542 37.0 35.0 37.0 33.0 37.0 6 35.679223658832385 37.0 35.0 37.0 33.0 37.0 7 35.60162289005917 37.0 35.0 37.0 33.0 37.0 8 35.58824047846877 37.0 35.0 37.0 33.0 37.0 9 37.226038789835535 39.0 37.0 39.0 34.0 39.0 10-11 37.133857634035095 39.0 37.0 39.0 33.5 39.0 12-13 37.0298769050889 39.0 37.0 39.0 33.0 39.0 14-15 38.280941318429726 40.0 38.0 41.0 33.0 41.0 16-17 38.2234483657444 40.0 38.0 41.0 33.0 41.0 18-19 38.16487483615249 40.0 38.0 41.0 33.0 41.0 20-21 38.07001614314862 40.0 38.0 41.0 33.0 41.0 22-23 37.99407800979206 40.0 38.0 41.0 33.0 41.0 24-25 38.01447625963519 40.0 38.0 41.0 33.0 41.0 26-27 37.93517610460107 40.0 38.0 41.0 33.0 41.0 28-29 37.797094845762146 40.0 38.0 41.0 33.0 41.0 30-31 37.61327120625799 40.0 37.5 41.0 32.0 41.0 32-33 37.41897051357263 40.0 37.0 41.0 32.0 41.0 34-35 37.119364530262956 40.0 36.5 41.0 31.0 41.0 36-37 36.87648925990605 40.0 36.0 41.0 30.5 41.0 38-39 36.568464100550315 39.5 35.5 41.0 30.0 41.0 40-41 36.436792291034024 39.0 35.5 41.0 30.0 41.0 42-43 36.265214849518905 39.0 35.0 41.0 30.0 41.0 44-45 36.21976120118093 39.0 35.0 41.0 30.0 41.0 46-47 36.01240562179026 39.0 35.0 41.0 28.5 41.0 48-49 35.7975312620376 39.0 35.0 41.0 27.5 41.0 50-51 34.64154706762291 38.0 34.0 40.0 25.5 40.5 52-53 34.75775681013561 38.5 34.5 39.5 26.0 40.5 54-55 35.248108182235185 39.0 35.0 40.5 26.0 41.0 56-57 35.149665261060306 39.0 35.0 41.0 26.0 41.0 58-59 34.947899553136956 38.5 35.0 40.5 26.0 41.0 60-61 34.6614617716357 38.0 34.0 40.0 26.0 41.0 62-63 34.308809177366385 37.5 34.0 40.0 24.5 41.0 64-65 33.931583029004585 37.0 34.0 40.0 23.0 41.0 66-67 33.57740306284258 36.5 33.5 39.5 22.5 41.0 68-69 33.170465388191005 36.0 33.0 39.0 22.0 41.0 70-71 32.759803287703306 35.5 33.0 39.0 20.5 40.5 72-73 32.33206633663501 35.0 32.0 38.0 19.5 40.0 74-75 31.889232445006442 35.0 32.0 37.0 18.0 39.5 76-77 31.457504624481235 35.0 31.5 37.0 16.5 39.0 78-79 31.039732766916618 35.0 31.0 36.0 12.0 39.0 80-81 30.58956078657696 35.0 31.0 36.0 7.5 37.5 82-83 30.168837461905028 34.0 30.5 35.0 4.5 37.0 84-85 29.762361044377325 34.0 30.0 35.0 2.0 36.5 86-87 29.444805846650976 34.0 30.0 35.0 2.0 36.0 88-89 29.059512547691025 34.0 29.5 35.0 2.0 36.0 90-91 28.679322171512105 34.0 29.0 35.0 2.0 35.5 92-93 28.27992709721415 34.0 29.0 35.0 2.0 35.0 94-95 27.82860393070696 34.0 28.0 35.0 2.0 35.0 96-97 27.282950194439188 34.0 27.0 35.0 2.0 35.0 98-99 26.577456147395385 33.5 25.5 35.0 2.0 35.0 100-101 24.173545653395983 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21946742655212859 1101 2 0.06074235352814128 1101 3 0.30088047076657176 1101 4 0.3384495683160793 1101 5 0.2962926223133522 1101 6 0.33283261336271863 1101 7 0.3064908162712996 1101 8 0.2362330004363855 1101 9 0.33768188606013183 1101 10-11 0.34087748237088533 1101 12-13 0.33152046123015566 1101 14-15 0.3987520853044302 1101 16-17 0.4542834372151745 1101 18-19 0.4302977112046875 1101 20-21 0.5102067110114916 1101 22-23 0.4011508565190809 1101 24-25 0.4899946390828873 1101 26-27 0.4073549035963211 1101 28-29 0.3841466184384572 1101 30-31 0.5408114835319822 1101 32-33 0.609997234822238 1101 34-35 0.5422601436181438 1101 36-37 0.6261172164018802 1101 38-39 0.7574555241682148 1101 40-41 0.7329815540091786 1101 42-43 0.5279464629299824 1101 44-45 0.6455227942845667 1101 46-47 0.5871456421917287 1101 48-49 0.6741017976020842 1101 50-51 0.7821600517588791 1101 52-53 0.8289009647111669 1101 54-55 0.7823336009922812 1101 56-57 0.752647784049401 1101 58-59 0.6963807646706215 1101 60-61 0.8186295577517129 1101 62-63 0.8519864175425624 1101 64-65 0.813178068264854 1101 66-67 0.8051467556327552 1101 68-69 0.8461285230454223 1101 70-71 0.623552506199502 1101 72-73 0.7457006791379044 1101 74-75 0.6518012702586304 1101 76-77 0.5668463566300339 1101 78-79 0.48842061915304313 1101 80-81 0.49613411211246117 1101 82-83 0.555400491053927 1101 84-85 0.4789935166076056 1101 86-87 0.4772377032223645 1101 88-89 0.4814823469405596 1101 90-91 0.4624667100877815 1101 92-93 0.4602097474041784 1101 94-95 0.4164013275684013 1101 96-97 0.32413607114344956 1101 98-99 0.26738859888437005 1101 100-101 -0.024987320903829158 1102 1 0.36950602384568043 1102 2 0.34804433107350974 1102 3 0.40987011568866194 1102 4 0.3721676137972736 1102 5 0.37461590086546437 1102 6 0.33108298400562575 1102 7 0.33320318075812594 1102 8 0.41517458069611735 1102 9 0.379917411501971 1102 10-11 0.39749357999252766 1102 12-13 0.4768321078703579 1102 14-15 0.5984594106523957 1102 16-17 0.5640289816204032 1102 18-19 0.5630206800615127 1102 20-21 0.5865303517535025 1102 22-23 0.5905539652774792 1102 24-25 0.6824489971385503 1102 26-27 0.616106231005098 1102 28-29 0.6533038564195763 1102 30-31 0.5459308543301873 1102 32-33 0.48592642096664207 1102 34-35 0.5823424443950742 1102 36-37 0.6400633899401242 1102 38-39 0.6874193452945434 1102 40-41 0.6097291059321392 1102 42-43 0.6339441728812076 1102 44-45 0.6087183980327708 1102 46-47 0.5732678090730161 1102 48-49 0.5646188695260079 1102 50-51 0.447126660945095 1102 52-53 0.533581810653807 1102 54-55 0.5916459215807635 1102 56-57 0.5868994865424995 1102 58-59 0.6027049613053563 1102 60-61 0.6011822343178537 1102 62-63 0.4916196486947939 1102 64-65 0.5543571396489284 1102 66-67 0.4845850575581494 1102 68-69 0.4296519774200931 1102 70-71 0.44619063551160565 1102 72-73 0.43179536658005446 1102 74-75 0.3889077111067749 1102 76-77 0.34160994462527583 1102 78-79 0.2621275811649362 1102 80-81 0.3063589277365999 1102 82-83 0.06013188311818851 1102 84-85 0.15292919695439267 1102 86-87 0.14499881950079896 1102 88-89 -0.07952215120579709 1102 90-91 -0.13247675791485847 1102 92-93 -0.12018895948619956 1102 94-95 -0.23427597326870497 1102 96-97 -0.36642638913457404 1102 98-99 -0.43666858700328604 1102 100-101 -0.651296319316959 1103 1 0.2369762916769247 1103 2 0.3257626624193293 1103 3 0.29002288585657965 1103 4 0.3181501451409403 1103 5 0.30269467096892555 1103 6 0.20819539147868937 1103 7 0.22185929747367084 1103 8 0.35010759602685226 1103 9 0.46393563219147893 1103 10-11 0.43522415873577813 1103 12-13 0.4117812381977046 1103 14-15 0.5717434320960848 1103 16-17 0.546990058531911 1103 18-19 0.6024809351081899 1103 20-21 0.644877382833954 1103 22-23 0.6574876037214139 1103 24-25 0.5409754418479906 1103 26-27 0.5058125083334417 1103 28-29 0.5359786235578738 1103 30-31 0.5129461420903141 1103 32-33 0.4180151393946758 1103 34-35 0.40425561679331423 1103 36-37 0.4643454668408893 1103 38-39 0.6327599626063503 1103 40-41 0.6512547663784076 1103 42-43 0.5974114755530735 1103 44-45 0.5944297677000563 1103 46-47 0.6492097306252091 1103 48-49 0.49308171371547616 1103 50-51 0.41329859721565043 1103 52-53 0.5230426028670365 1103 54-55 0.5824576876769569 1103 56-57 0.4481010206901743 1103 58-59 0.4464942416096065 1103 60-61 0.4982745517618028 1103 62-63 0.456376151095931 1103 64-65 0.5345330079786663 1103 66-67 0.32709319563144135 1103 68-69 0.37251894275136976 1103 70-71 0.2767172736010153 1103 72-73 0.33743668972871177 1103 74-75 0.3440274455316299 1103 76-77 0.38629145857548863 1103 78-79 0.37800783599675114 1103 80-81 0.33476698093757307 1103 82-83 0.321017563332191 1103 84-85 0.19268757943846637 1103 86-87 0.18960513013347224 1103 88-89 0.10279754884040315 1103 90-91 0.07354810215325358 1103 92-93 0.18164224129591844 1103 94-95 0.11865051011673344 1103 96-97 0.05124477898781521 1103 98-99 -0.18495233986370252 1103 100-101 -0.2622319560204751 1104 1 0.09980774433046236 1104 2 0.16106068839192744 1104 3 0.24568332019751438 1104 4 0.16193539072752117 1104 5 0.18831282433355767 1104 6 0.10307637327590413 1104 7 0.19345828712307167 1104 8 0.2438346366330606 1104 9 0.21303208223677927 1104 10-11 0.24848857956876458 1104 12-13 0.2255086906316066 1104 14-15 0.1940480198565595 1104 16-17 0.2454630014525918 1104 18-19 0.10225154708413697 1104 20-21 0.13450687518769655 1104 22-23 0.20272152185006576 1104 24-25 0.2529448018677627 1104 26-27 0.1969461326916786 1104 28-29 0.24570448359136066 1104 30-31 0.24968117215373553 1104 32-33 0.21793167564923266 1104 34-35 0.23829125361619674 1104 36-37 0.2838899854143193 1104 38-39 0.35166038267059463 1104 40-41 0.1363461921258775 1104 42-43 0.130444723775355 1104 44-45 0.15045837599701173 1104 46-47 0.22609686238261872 1104 48-49 0.16839291817419877 1104 50-51 0.18526548626927308 1104 52-53 0.08207151317097328 1104 54-55 0.1723739493797325 1104 56-57 0.2050412782197597 1104 58-59 0.24932967526416405 1104 60-61 0.2598016088411228 1104 62-63 0.19597255995846297 1104 64-65 0.31046401311277805 1104 66-67 0.24639199034906056 1104 68-69 0.2835734961928722 1104 70-71 0.06536387053152737 1104 72-73 0.03080502841580568 1104 74-75 -0.055988890300120886 1104 76-77 -0.020295725346755944 1104 78-79 -0.07992259257860113 1104 80-81 -0.05502847797574972 1104 82-83 -0.1575200365360203 1104 84-85 -0.20211995975380148 1104 86-87 -0.1845960614213169 1104 88-89 -0.17180242919545563 1104 90-91 -0.1679704438204439 1104 92-93 -0.1602105714872124 1104 94-95 -0.31688678774450807 1104 96-97 -0.10921687985412731 1104 98-99 -0.08545916247578589 1104 100-101 -0.0936732156641078 1105 1 0.22491526269048023 1105 2 0.2951448010352422 1105 3 0.27611709205782375 1105 4 0.24349559572566903 1105 5 0.2912673337645302 1105 6 0.28539528277027415 1105 7 0.24961087337551646 1105 8 0.31527860556633414 1105 9 0.43636858564460823 1105 10-11 0.35699256857917305 1105 12-13 0.32811449186793595 1105 14-15 0.3805118017554392 1105 16-17 0.35796656778639857 1105 18-19 0.34220082589219203 1105 20-21 0.4713682372630359 1105 22-23 0.38715011592203297 1105 24-25 0.2923361475643844 1105 26-27 0.35325246755721196 1105 28-29 0.5062962308307632 1105 30-31 0.45812545809445027 1105 32-33 0.33868430416934103 1105 34-35 0.38856361585003896 1105 36-37 0.33500188904405803 1105 38-39 0.5832414573258475 1105 40-41 0.5687237479984404 1105 42-43 0.47564179673990026 1105 44-45 0.48911373606463826 1105 46-47 0.5513311837224961 1105 48-49 0.564955236555349 1105 50-51 0.48265017480945716 1105 52-53 0.4563653790768143 1105 54-55 0.4922355227933721 1105 56-57 0.46705638890819046 1105 58-59 0.46191883360938135 1105 60-61 0.4736746473575266 1105 62-63 0.5270773309205765 1105 64-65 0.4179947892526883 1105 66-67 0.39134338254011425 1105 68-69 0.3011589561178454 1105 70-71 0.260250804541144 1105 72-73 0.43016467336746445 1105 74-75 0.4936930455587003 1105 76-77 0.36288702944798246 1105 78-79 0.39910472289664867 1105 80-81 0.4400700585065245 1105 82-83 0.3615915500862634 1105 84-85 0.32109026901990845 1105 86-87 0.22609210891735643 1105 88-89 0.31757755706755475 1105 90-91 0.2915210233957026 1105 92-93 0.4135832151667742 1105 94-95 0.34229982397420144 1105 96-97 0.4344247432240138 1105 98-99 0.47142201256516003 1105 100-101 0.09039134818647909 1106 1 0.14117156017189103 1106 2 0.18278855698547147 1106 3 0.12137470454561594 1106 4 0.2608542883031362 1106 5 0.20738650355649213 1106 6 0.21329906931949694 1106 7 0.15874692330722695 1106 8 0.19611134831275479 1106 9 0.19936707394490583 1106 10-11 0.28888737922129337 1106 12-13 0.2611838860829252 1106 14-15 0.30731501819818874 1106 16-17 0.3474032999600567 1106 18-19 0.3485995688799264 1106 20-21 0.28239639583840415 1106 22-23 0.37946044627555864 1106 24-25 0.34211324536455834 1106 26-27 0.3171222363006194 1106 28-29 0.27978448043255355 1106 30-31 0.2607093645252121 1106 32-33 0.26784796720731663 1106 34-35 0.37208814218920594 1106 36-37 0.3027149084183449 1106 38-39 0.4841479448596502 1106 40-41 0.5388941665395279 1106 42-43 0.3257365187686361 1106 44-45 0.40700263880992793 1106 46-47 0.206077119825558 1106 48-49 0.24583033426833367 1106 50-51 0.31877650261611024 1106 52-53 0.26662019831667294 1106 54-55 0.3672432536520205 1106 56-57 0.4006043359469871 1106 58-59 0.3720553326913105 1106 60-61 0.3355656457991856 1106 62-63 0.31735611835694044 1106 64-65 0.253188091135371 1106 66-67 0.2001178057166655 1106 68-69 0.14016040292904108 1106 70-71 0.2710517012193563 1106 72-73 0.07339327764584169 1106 74-75 0.1974182801966009 1106 76-77 0.27895208322940945 1106 78-79 0.44485036801525624 1106 80-81 0.3934134394213906 1106 82-83 0.38676449319626727 1106 84-85 0.42905551209330994 1106 86-87 0.46327016301207635 1106 88-89 0.5177383589274207 1106 90-91 0.45972283581313533 1106 92-93 0.5197195398608088 1106 94-95 0.4416281771137385 1106 96-97 0.3636840914796835 1106 98-99 0.4730546178290389 1106 100-101 0.35265743268147176 1107 1 0.05452853805546454 1107 2 0.04086069878100318 1107 3 0.06870602031699491 1107 4 0.1270395411612668 1107 5 0.10354112416213468 1107 6 0.08293456246087771 1107 7 0.1301446930240644 1107 8 0.033085065182227424 1107 9 0.17541949068309037 1107 10-11 0.11761304063571743 1107 12-13 0.13914356674194295 1107 14-15 0.24447267312132936 1107 16-17 0.14166917256059008 1107 18-19 0.1425616608444642 1107 20-21 0.2931843464878767 1107 22-23 0.17261087824714139 1107 24-25 0.16717808845027804 1107 26-27 0.20994311986117253 1107 28-29 0.25196961267556617 1107 30-31 0.22690573191686525 1107 32-33 0.17578087828805877 1107 34-35 0.3064326488683946 1107 36-37 0.4297400711241508 1107 38-39 0.48931582914091365 1107 40-41 0.36654488310296784 1107 42-43 0.4033765195262262 1107 44-45 0.5665011789682879 1107 46-47 0.5050423717068071 1107 48-49 0.48421053803810565 1107 50-51 0.4614810075408968 1107 52-53 0.4871416393981747 1107 54-55 0.5486536231629131 1107 56-57 0.4531429352765315 1107 58-59 0.4484180343104427 1107 60-61 0.392725872515733 1107 62-63 0.4316894058365932 1107 64-65 0.5221052265562349 1107 66-67 0.32313787367980495 1107 68-69 0.5111029751809752 1107 70-71 0.4341953598048107 1107 72-73 0.45499546008169744 1107 74-75 0.46731067855344577 1107 76-77 0.45765347209753315 1107 78-79 0.3926515594135047 1107 80-81 0.2415075461268934 1107 82-83 0.33645689972702897 1107 84-85 0.11748161938494306 1107 86-87 0.21364870508432432 1107 88-89 0.22890714433318848 1107 90-91 0.4232305327521999 1107 92-93 0.49310795742037783 1107 94-95 0.3601637550844359 1107 96-97 0.22341207448590694 1107 98-99 0.3243855209172857 1107 100-101 0.21545462185082798 1108 1 0.1837177667809513 1108 2 0.2805020600039754 1108 3 0.16256082079326717 1108 4 0.18779822952296854 1108 5 0.2436189160670139 1108 6 0.1874632491460133 1108 7 0.2243828961208294 1108 8 0.23904553327301414 1108 9 0.3895885032022761 1108 10-11 0.3162864248234314 1108 12-13 0.33954131282372657 1108 14-15 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-0.8777731948693983 2316 60-61 -0.7998886440716362 2316 62-63 -0.8998529586547761 2316 64-65 -1.1407640819899925 2316 66-67 -0.9199033587084315 2316 68-69 -0.9247773610582328 2316 70-71 -0.9472936079187875 2316 72-73 -1.0682046334199455 2316 74-75 -1.1310665638816317 2316 76-77 -0.8181431251762525 2316 78-79 -0.8366669328267768 2316 80-81 -0.6778919808648283 2316 82-83 -0.6240375130918636 2316 84-85 -0.5518239476291456 2316 86-87 -0.36464058164486346 2316 88-89 -0.5553114753259862 2316 90-91 -0.6521780785651643 2316 92-93 -0.6227240696400997 2316 94-95 -0.6704270927553999 2316 96-97 -0.7188403524315632 2316 98-99 -0.7471821898509852 2316 100-101 -0.19997566901259134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9653.0 3 2615.0 4 2562.0 5 2787.0 6 3238.0 7 3727.0 8 4669.0 9 5653.0 10 6996.0 11 8905.0 12 11324.0 13 16327.0 14 15066.0 15 16187.0 16 20170.0 17 27317.0 18 23384.0 19 16519.0 20 16393.0 21 17498.0 22 18574.0 23 20257.0 24 23355.0 25 26654.0 26 30967.0 27 35825.0 28 42658.0 29 50783.0 30 61407.0 31 75250.0 32 94733.0 33 122792.0 34 169616.0 35 248594.0 36 388802.0 37 612702.0 38 585862.0 39 98865.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07579493640304 18.543818329109914 14.174372441977201 23.206014292509845 2 15.255120116641912 20.11452982135801 40.35724992164825 24.273100140351833 3 19.198915497597927 24.40968515974027 29.76095770910792 26.630441633553886 4 12.779715991169017 16.315380631796998 36.4831080160266 34.42179536100738 5 13.955588174064266 37.28711227047649 33.66615115339705 15.09114840206219 6 32.29669625130703 35.387472305896125 17.470395781813874 14.84543566098297 7 28.91706653990583 32.87315562299184 20.237649331032387 17.972128506069946 8 26.667136591958762 35.040183720357675 19.63041397353503 18.662265714148532 9 27.04134621212689 16.76377066340134 20.140538029920517 36.05434509455125 10-11 25.37628828769931 26.514431818646138 26.77489849648126 21.334381397173292 12-13 26.088063939393578 23.81540825244231 27.623604373384786 22.47292343477933 14-15 22.979619215924625 25.1194568302271 26.043614980072082 25.85730897377619 16-17 22.54423417493725 28.567736522550124 26.53223068680233 22.355798615710295 18-19 22.351447893539643 26.764407426072978 29.184878842774836 21.69926583761254 20-21 23.119475373884647 25.99804022894076 29.109887649441056 21.772596747733537 22-23 22.709046456433065 25.93486883756919 29.115992427258462 22.24009227873928 24-25 22.514138870014357 26.133740682650703 29.16713072473097 22.18498972260397 26-27 22.326899968937838 26.47516321259053 29.006150444406902 22.19178637406473 28-29 22.11212512300994 26.484969841598787 29.315301014407343 22.087604020983935 30-31 22.23075321142362 26.31292519786872 29.128679499930783 22.327642090776877 32-33 22.036656618120926 26.424145114508303 29.06533200639179 22.47386626097898 34-35 22.709989801104484 26.39071661737769 28.7024548980281 22.196838683489727 36-37 22.694755823613434 26.210875298726204 28.743544141465797 22.350824736194557 38-39 22.383920479192554 26.51759986492962 28.892786521369274 22.205693134508554 40-41 22.455900372828687 26.284123690435614 29.055478472015146 22.204497464720554 42-43 22.32075405018226 26.333453353909263 28.68286893223857 22.662923663669908 44-45 22.367323549836193 26.75485439752812 28.399051531119525 22.478770521516164 46-47 22.488628743524185 26.8884516324799 27.997615523888904 22.625304100107012 48-49 22.74750269954196 26.849781825093586 27.870954123924985 22.531761351439464 50-51 22.591212182480376 27.068317436291156 27.767239706995333 22.57323067423313 52-53 22.77425117909526 27.002190754636334 27.69739546271815 22.526162603550258 54-55 22.280019751251697 27.0162223887131 27.81284596277449 22.89091189726071 56-57 22.513885764091714 27.38488787772539 27.644355102747316 22.45687125543558 58-59 22.534397543332524 26.874869644857117 27.92080005543614 22.669932756374216 60-61 22.55364942689042 26.84558651799102 28.140160593856407 22.460603461262153 62-63 22.522366411014772 26.90490937685448 28.173568539451676 22.399155672679072 64-65 22.296249339528135 26.697408422822217 28.163186331451506 22.843155906198138 66-67 22.299472574217976 27.080136509398727 27.832482070887814 22.787908845495483 68-69 22.29317520271616 26.949147852559758 28.028495895119477 22.72918104960461 70-71 22.22205901015716 26.938758639018616 27.983596731985845 22.855585618838376 72-73 22.365379892773205 26.896594830339232 27.766049266124575 22.971976010762983 74-75 22.248093735867826 27.17324699526867 27.560033145291413 23.018626123572094 76-77 22.44165835798333 26.802210192299846 27.700179788580648 23.055951661136177 78-79 22.326747902496518 27.0076641290286 27.717245638971622 22.94834232950326 80-81 22.24329226155195 27.37198414671121 27.61156574444083 22.77315784729601 82-83 22.403585630909557 27.145060392529675 27.474772205862692 22.976581770698076 84-85 22.329639872168862 26.9744296740907 27.404398139768695 23.291532313971747 86-87 22.283990203631156 26.926167314112128 27.723129647538343 23.066712834718377 88-89 22.223726859303095 26.81423798034519 27.730396888919017 23.231638271432697 90-91 22.17312170006231 26.93806433717028 27.85239645164464 23.03641751112277 92-93 22.268910146487965 26.615333481811636 27.831261468924083 23.284494902776316 94-95 22.048863152702562 26.550991603022666 27.980616106264698 23.41952913801007 96-97 22.203369276150493 26.381555237101324 27.934401084945865 23.48067440180232 98-99 22.04441349049484 26.394084804979016 27.736630771508786 23.824870933017355 100-101 22.170782982147113 25.987649346063506 28.29642184145839 23.545145830330988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2889.0 1 1719.0 2 723.0 3 900.0 4 756.5 5 756.0 6 1329.5 7 2655.0 8 4232.0 9 4957.5 10 4472.5 11 3949.5 12 4119.0 13 4366.0 14 4567.5 15 4826.0 16 5076.0 17 5269.5 18 5455.5 19 5672.0 20 5944.5 21 6736.5 22 7898.0 23 9484.0 24 11419.5 25 13754.5 26 17100.0 27 20690.0 28 23728.0 29 27280.0 30 31515.5 31 36167.0 32 41400.5 33 45735.5 34 50521.0 35 55040.0 36 60312.0 37 67216.5 38 72785.0 39 76168.5 40 78282.0 41 84570.5 42 91833.5 43 96080.0 44 100617.0 45 105444.0 46 106029.0 47 108177.5 48 120303.0 49 122815.5 50 114595.5 51 109636.0 52 105078.5 53 104444.5 54 104650.5 55 98286.0 56 87123.5 57 77213.5 58 68924.0 59 59289.0 60 50459.0 61 42726.5 62 35349.5 63 27499.5 64 20761.5 65 16243.5 66 12461.0 67 10372.0 68 7974.5 69 5296.5 70 3977.0 71 3171.0 72 2563.5 73 2112.5 74 1695.5 75 1384.0 76 1030.5 77 636.5 78 392.0 79 269.5 80 194.5 81 140.0 82 105.5 83 84.0 84 60.5 85 46.0 86 32.5 87 23.0 88 24.0 89 22.0 90 16.5 91 13.5 92 8.5 93 6.0 94 5.5 95 4.5 96 2.5 97 1.5 98 2.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2797828161219148 2 0.10984419003180991 3 0.12035901491403704 4 0.12209447144799687 5 0.12825364071557976 6 0.058222865286377545 7 0.06615151964740967 8 0.07047314670256452 9 0.06785294762188009 10-11 0.06342923488825701 12-13 0.06769981910417774 14-15 0.06373549192366168 16-17 0.06448412023242867 18-19 0.06900991864451998 20-21 0.0728381315870784 22-23 0.08122617150121754 24-25 0.09027776832540015 26-27 0.090515968241826 28-29 0.08376129918317846 30-31 0.08012024331781177 32-33 0.07249784599218431 34-35 0.23881243049666723 36-37 0.36242117284194275 38-39 0.3356747250832679 40-41 0.3180479312677544 42-43 0.341765837231872 44-45 0.3542202900049954 46-47 0.3607537734269618 48-49 0.3707921984763372 50-51 0.36410558653666847 52-53 0.36483720056569074 54-55 0.3500177629080535 56-57 0.3276609993235122 58-59 0.31343706145693956 60-61 0.26877457712709124 62-63 0.339809195061231 64-65 0.37012864156629377 66-67 0.3742290829847675 68-69 0.3858498360504004 70-71 0.3867175643173803 72-73 0.37074115563710314 74-75 0.3740419259075757 76-77 0.35888220265504434 78-79 0.3358278536009702 80-81 0.3352493680896503 82-83 0.3485034920107748 84-85 0.3562790178541046 86-87 0.34967747731315935 88-89 0.36487122912518016 90-91 0.3872450069894661 92-93 0.3822087801850337 94-95 0.3820046088280972 96-97 0.3870068070730403 98-99 0.3764069107920896 100-101 0.35532621818840116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2938708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40830875635461 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44722370551449 33.87192041215369 2 14.692657098834975 13.931080483856276 3 5.679499950582361 8.07766461716728 4 2.7005682509140687 5.121174938277708 5 1.4356850662224703 3.4031700448181144 6 0.9337452464144205 2.656036976507596 7 0.6073146323785169 2.01542317228369 8 0.44305277297801915 1.6803506125360812 9 0.3104707214722319 1.324700264102737 >10 1.5427455919451447 13.038744847919542 >50 0.12061740844697812 3.941608092175726 >100 0.07915394634018497 7.1461382459851785 >500 0.005754936995001245 1.9082881304674837 >1k 0.0012948608238328135 0.9217623373963378 >5k 1.4387342487031263E-4 0.5531665351697721 >10k+ 7.193671243515632E-5 0.40877028918279207 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11986 0.4078663140400476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9668 0.32898811314359916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6552 0.22295512177460297 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.4028559489408274E-5 8 0.0 0.0 0.0 0.0 3.4028559489408274E-5 9 0.0 0.0 0.0 0.0 3.4028559489408274E-5 10-11 0.0 0.0 0.0 0.0 6.805711897881655E-5 12-13 0.0 0.0 0.0 1.7014279744704137E-5 4.423712733623075E-4 14-15 0.0 0.0 0.0 6.805711897881655E-5 4.934141125964199E-4 16-17 0.0 0.0 0.0 6.805711897881655E-5 5.10428392341124E-4 18-19 0.0 0.0 0.0 6.805711897881655E-5 5.444569518305324E-4 20-21 0.0 0.0 0.0 6.805711897881655E-5 5.784855113199406E-4 22-23 0.0 0.0 0.0 1.7014279744704134E-4 6.125140708093488E-4 24-25 0.0 0.0 0.0 3.402855948940827E-4 6.125140708093488E-4 26-27 0.0 0.0 0.0 6.29528350554053E-4 6.805711897881654E-4 28-29 0.0 0.0 0.0 0.001888585051662159 6.805711897881654E-4 30-31 0.0 0.0 0.0 0.005989026470135855 6.805711897881654E-4 32-33 0.0 0.0 0.0 0.014206923586827952 7.145997492775737E-4 34-35 0.0 0.0 0.0 0.028005504459783004 7.145997492775737E-4 36-37 0.0 0.0 0.0 0.05092373927589948 7.145997492775737E-4 38-39 0.0 0.0 0.0 0.08607524122845822 7.145997492775737E-4 40-41 0.0 0.0 0.0 0.12820259787634566 7.145997492775737E-4 42-43 0.0 0.0 0.0 0.17058516872040366 7.486283087669819E-4 44-45 0.0 0.0 0.0 0.22486072110600985 7.486283087669819E-4 46-47 0.0 0.0 0.0 0.29193101185963355 7.826568682563902E-4 48-49 0.0 0.0 0.0 0.3669639855337788 8.166854277457985E-4 50-51 0.0 0.0 0.0 0.448785656826061 8.507139872352068E-4 52-53 0.0 0.0 0.0 0.5320365276168983 8.84742546724615E-4 54-55 0.0 0.0 0.0 0.6280481082162638 9.868282251928398E-4 56-57 0.0 0.0 0.0 0.7427413679753143 0.0010378710644269522 58-59 0.0 0.0 0.0 0.872322122510981 0.0010889139036610648 60-61 0.0 0.0 0.0 1.0209418560809715 0.0010889139036610648 62-63 0.0 0.0 0.0 1.1737471024681594 0.0011569710226398812 64-65 0.0 0.0 0.0 1.3320139326533973 0.0011569710226398812 66-67 0.0 0.0 0.0 1.519222052684377 0.0012080138618739936 68-69 0.0 0.0 0.0 1.732683206361435 0.0012930852605975143 70-71 0.0 0.0 0.0 1.9772804919713018 0.001378156659321035 72-73 0.0 0.0 0.0 2.22723727570075 0.0014632280580445557 74-75 0.0 0.0 0.0 2.4857011993025506 0.0015482994567680764 76-77 0.0 0.0 0.0 2.769958770997323 0.0015653137365127805 78-79 0.0 0.0 0.0 3.0940637858541917 0.001633370855491597 80-81 0.0 1.7014279744704137E-5 0.0 3.4523334744384266 0.001633370855491597 82-83 0.0 3.4028559489408274E-5 0.0 3.8215263306187617 0.0016673994149810053 84-85 0.0 3.4028559489408274E-5 0.0 4.201097897443367 0.0016673994149810053 86-87 0.0 3.4028559489408274E-5 0.0 4.6078412690202635 0.0016844136947257093 88-89 0.0 3.4028559489408274E-5 0.0 5.0502635852218045 0.0017014279744704136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9610 0.0 33.855553 1 GTATCAA 14650 0.0 32.453323 1 ATCAACG 18060 0.0 26.17935 3 TCAACGC 18150 0.0 25.99728 4 TATCAAC 18180 0.0 25.954378 2 CAACGCA 18365 0.0 25.718752 5 GTACATG 13665 0.0 25.268967 1 AACGCAG 18880 0.0 25.053356 6 TACATGG 13775 0.0 24.47707 2 ACATGGG 14100 0.0 22.971167 3 ACGCAGA 21040 0.0 22.369087 7 CGCAGAG 21330 0.0 21.998297 8 GAGTACT 10205 0.0 21.898453 12-13 AGTACTT 10760 0.0 20.96715 12-13 CATGGGG 6955 0.0 20.796207 4 GCAGAGT 22485 0.0 20.467796 9 GTACTTT 11145 0.0 19.967104 14-15 GTATAGG 720 0.0 19.130585 1 AGAGTAC 19720 0.0 18.65073 10-11 TACCTGG 2500 0.0 18.589493 2 >>END_MODULE