FastQCFastQC Report
Fri 27 May 2016
SRR939340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938708
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119070.40517805784038424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104010.35393104724933544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.24044580135215882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100500.033.4231571
GTATCAA150900.031.1513251
ATCAACG184900.025.1490463
TATCAAC187200.025.0428312
GTACATG138600.025.0249461
TCAACGC186200.024.9734614
CAACGCA187800.024.7101635
TACATGG138900.024.5958942
AACGCAG192750.024.1248176
ACATGGG142800.023.1931423
GAGTACT105600.021.81623812-13
ACGCAGA215100.021.5298867
CGCAGAG217050.021.336468
AGTACTT110600.020.636912-13
CATGGGG64250.020.0876034
GTACTTT114050.019.86701414-15
GCAGAGT231750.019.5326429
CATGGGA80050.018.9679764
AGAGTAC196800.018.63793610-11
AGTACAT106800.018.0379472
Produced by FastQC (version 0.11.5)