##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2938708 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.263418141577866 33.0 31.0 34.0 31.0 34.0 2 32.46369935359348 34.0 31.0 34.0 31.0 34.0 3 32.54817321081237 34.0 31.0 34.0 31.0 34.0 4 36.095358572542764 37.0 35.0 37.0 35.0 37.0 5 36.03291514502291 37.0 35.0 37.0 35.0 37.0 6 36.072139865546355 37.0 35.0 37.0 35.0 37.0 7 36.0138353317172 37.0 35.0 37.0 35.0 37.0 8 35.991816131442796 37.0 35.0 37.0 35.0 37.0 9 37.691132974082485 39.0 37.0 39.0 35.0 39.0 10-11 37.60758418325332 39.0 37.0 39.0 35.0 39.0 12-13 37.52585047578732 39.0 37.0 39.0 35.0 39.0 14-15 38.810178656743034 40.0 38.0 41.0 35.0 41.0 16-17 38.756794992901646 40.0 38.0 41.0 34.0 41.0 18-19 38.87209957573192 40.0 38.0 41.0 35.0 41.0 20-21 38.87928657763888 40.0 38.0 41.0 35.0 41.0 22-23 38.8236095590307 40.0 38.0 41.0 34.5 41.0 24-25 38.75336083067798 40.0 38.0 41.0 34.0 41.0 26-27 38.588777789423105 40.0 38.0 41.0 34.0 41.0 28-29 38.435716988554155 40.0 38.0 41.0 34.0 41.0 30-31 38.238756283373505 40.0 38.0 41.0 34.0 41.0 32-33 38.03371872945526 40.0 37.0 41.0 33.0 41.0 34-35 37.98494559513909 40.0 37.0 41.0 33.0 41.0 36-37 37.89813210431251 40.0 37.0 41.0 33.0 41.0 38-39 37.79621758949851 40.0 37.0 41.0 33.0 41.0 40-41 37.61454098195534 40.0 37.0 41.0 33.0 41.0 42-43 37.43144317162508 40.0 37.0 41.0 32.0 41.0 44-45 37.191897425671414 40.0 36.5 41.0 31.5 41.0 46-47 36.878254321286775 40.0 36.0 41.0 31.0 41.0 48-49 36.54870950090992 39.5 36.0 41.0 30.0 41.0 50-51 36.291990051410345 39.0 35.0 41.0 30.0 41.0 52-53 36.0535425772142 39.0 35.0 41.0 29.5 41.0 54-55 35.7654149374487 39.0 35.0 40.0 28.5 41.0 56-57 35.5209131019482 38.5 35.0 40.0 28.0 41.0 58-59 35.22425382174752 38.0 34.5 40.0 28.0 41.0 60-61 34.89008349927927 38.0 34.0 40.0 27.0 41.0 62-63 34.50584321409272 37.0 34.0 40.0 26.0 41.0 64-65 34.07692972558009 36.5 33.5 39.5 26.0 41.0 66-67 33.70192394072497 36.0 33.0 39.0 26.0 40.5 68-69 33.320817005296206 36.0 33.0 39.0 25.0 40.0 70-71 32.924877020786 35.0 32.5 38.0 24.0 40.0 72-73 32.433708622973086 35.0 32.0 37.5 22.5 39.5 74-75 31.930260679182823 35.0 31.5 37.0 21.5 39.0 76-77 29.954438140842846 33.0 29.0 35.0 18.0 37.5 78-79 30.944041224919253 34.0 31.0 36.0 19.5 38.0 80-81 30.994685759864538 34.0 31.0 35.5 20.0 37.0 82-83 30.690861766463357 34.0 31.0 35.0 18.0 37.0 84-85 30.317960818155463 34.0 31.0 35.0 16.5 36.5 86-87 29.787224862082248 34.0 30.0 35.0 8.5 36.0 88-89 29.28976339262016 34.0 29.0 35.0 3.5 36.0 90-91 28.873368670858078 34.0 29.0 35.0 2.0 35.0 92-93 28.521072866035006 34.0 29.0 35.0 2.0 35.0 94-95 28.115428446786822 34.0 29.0 35.0 2.0 35.0 96-97 27.555558088792765 33.0 27.0 35.0 2.0 35.0 98-99 26.791701659368677 33.0 26.5 35.0 2.0 35.0 100-101 23.424087217920256 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30459944851379106 1101 2 0.23970825842977916 1101 3 0.3044442830331917 1101 4 0.1638206470453909 1101 5 0.24733097619950684 1101 6 0.2380145945372476 1101 7 0.20559959644261028 1101 8 0.24522923020311538 1101 9 0.29713403451892617 1101 10-11 0.35836284513640493 1101 12-13 0.36690079540044707 1101 14-15 0.4488319425570353 1101 16-17 0.5199483580630542 1101 18-19 0.42672784313938195 1101 20-21 0.43660456411634385 1101 22-23 0.4175003692002477 1101 24-25 0.449965951863156 1101 26-27 0.45531999256980527 1101 28-29 0.4181479628751319 1101 30-31 0.4456796707030364 1101 32-33 0.33213696473620047 1101 34-35 0.47627652738324855 1101 36-37 0.4415586727519383 1101 38-39 0.4259856341551185 1101 40-41 0.5910520685311695 1101 42-43 0.4964179736338181 1101 44-45 0.6018887329052447 1101 46-47 0.6395804143167041 1101 48-49 0.7319640531355205 1101 50-51 0.8392816072823948 1101 52-53 0.7502278983730051 1101 54-55 0.8066933574939554 1101 56-57 0.7663422756702047 1101 58-59 0.6872154771056955 1101 60-61 0.730628686004053 1101 62-63 0.7793931771037848 1101 64-65 0.7370185216584346 1101 66-67 0.8755685412798044 1101 68-69 0.8204365635498263 1101 70-71 0.760659674070638 1101 72-73 0.5935907947243422 1101 74-75 0.5185074325811065 1101 76-77 0.5928865714195091 1101 78-79 0.784052961866962 1101 80-81 0.8211902911137337 1101 82-83 0.791904346053041 1101 84-85 0.809174265330995 1101 86-87 0.8473063321356804 1101 88-89 1.0640319460794032 1101 90-91 1.1473859041395222 1101 92-93 1.064316647248571 1101 94-95 1.2026659109200004 1101 96-97 1.207342220143051 1101 98-99 1.1238376665198366 1101 100-101 0.80906908478714 1102 1 0.18872246470746035 1102 2 0.16511433233142014 1102 3 0.19098715181787185 1102 4 0.14963449404652351 1102 5 0.1561585526046656 1102 6 0.1544782457437961 1102 7 0.15231441373443033 1102 8 0.2460292572527436 1102 9 0.34218942607657965 1102 10-11 0.28171841990941715 1102 12-13 0.28580378409748874 1102 14-15 0.42122685447669994 1102 16-17 0.3905941277480949 1102 18-19 0.3074285373175769 1102 20-21 0.3538695318143965 1102 22-23 0.18305834508596064 1102 24-25 0.24745480678718224 1102 26-27 0.3172982772224131 1102 28-29 0.35488765779891907 1102 30-31 0.3899759248457286 1102 32-33 0.38219037628422114 1102 34-35 0.4308655364099181 1102 36-37 0.5419986016664353 1102 38-39 0.3921566966724228 1102 40-41 0.41459123117333974 1102 42-43 0.409335659675925 1102 44-45 0.48045009973655084 1102 46-47 0.3424268142809481 1102 48-49 0.3358959339269063 1102 50-51 0.24415761741683895 1102 52-53 0.3323987282176759 1102 54-55 0.33332892855338514 1102 56-57 0.41299379569294814 1102 58-59 0.2823237071260749 1102 60-61 0.2929135999352397 1102 62-63 0.3856490619732469 1102 64-65 0.2832685094803722 1102 66-67 0.1451856801974003 1102 68-69 0.3028346790162004 1102 70-71 0.18474650565978123 1102 72-73 0.31145016647387536 1102 74-75 0.20247439765327258 1102 76-77 0.300071226946546 1102 78-79 0.3267538325554291 1102 80-81 0.4077483753125719 1102 82-83 0.39779789775478847 1102 84-85 0.39290417367746855 1102 86-87 0.47270054317204213 1102 88-89 0.6463438981740595 1102 90-91 0.7615876164037196 1102 92-93 0.647466163478633 1102 94-95 0.5246535884403265 1102 96-97 0.3040433406966976 1102 98-99 0.4878714647972586 1102 100-101 0.398468382528435 1103 1 0.19939059246715374 1103 2 0.22541127800239025 1103 3 0.20582906370106713 1103 4 0.1072144512880655 1103 5 0.11436162304868702 1103 6 0.15251758782220293 1103 7 0.18434028367565958 1103 8 0.13548575213354042 1103 9 0.14401256936038465 1103 10-11 0.26157467413960234 1103 12-13 0.19891440817759332 1103 14-15 0.2560937983213023 1103 16-17 0.2617386148077614 1103 18-19 0.21215130565912688 1103 20-21 0.38426510126465985 1103 22-23 0.24190225522874442 1103 24-25 0.23735876140735712 1103 26-27 0.23984691765387112 1103 28-29 0.2596533994647743 1103 30-31 0.29682855857333834 1103 32-33 0.3328167124860144 1103 34-35 0.4060618707853507 1103 36-37 0.3498729261795006 1103 38-39 0.31951029192816804 1103 40-41 0.37150510188808994 1103 42-43 0.36509730813264696 1103 44-45 0.29007290138243746 1103 46-47 0.22624574617896798 1103 48-49 0.2748630700771457 1103 50-51 0.26816359070891593 1103 52-53 0.2055441606904722 1103 54-55 0.16608749367408393 1103 56-57 0.27589814781637045 1103 58-59 0.010385825066371979 1103 60-61 0.013920035190558622 1103 62-63 0.0591216288773424 1103 64-65 0.21335628249527616 1103 66-67 0.16524885098413478 1103 68-69 0.1321070332023382 1103 70-71 0.25340982040714977 1103 72-73 0.2874549365128729 1103 74-75 0.2166448513441459 1103 76-77 0.262488369005613 1103 78-79 0.4581205991350714 1103 80-81 0.4377544990112874 1103 82-83 0.41829885928292043 1103 84-85 0.30071027803818495 1103 86-87 0.3430419662336739 1103 88-89 0.47039230762389295 1103 90-91 0.5676812337907293 1103 92-93 0.599558964372477 1103 94-95 0.3979118258894303 1103 96-97 0.2950941459093599 1103 98-99 0.13290106267437807 1103 100-101 0.31656928389391226 1104 1 0.1112616629445995 1104 2 0.11082159565881966 1104 3 0.1252629379014394 1104 4 0.08224149463979558 1104 5 0.07720186135964724 1104 6 0.03974818387414558 1104 7 0.11381369333439295 1104 8 0.08352819784092702 1104 9 0.1344938586556097 1104 10-11 0.16555306691339666 1104 12-13 0.17447655297620912 1104 14-15 0.18399361437160167 1104 16-17 0.12708111817101297 1104 18-19 0.13466771012883072 1104 20-21 0.16812667225002542 1104 22-23 0.21924912396553253 1104 24-25 0.14697891900008386 1104 26-27 0.15218047062862894 1104 28-29 0.1702313309915624 1104 30-31 0.13011576720525397 1104 32-33 0.10345065257882169 1104 34-35 0.21796698568527972 1104 36-37 0.17355466573966538 1104 38-39 0.31180581947943864 1104 40-41 0.37111551289213196 1104 42-43 0.39355254246260785 1104 44-45 0.3737652111866794 1104 46-47 0.4148507485126558 1104 48-49 0.3514514894824643 1104 50-51 0.25678877259947797 1104 52-53 0.24494852057296868 1104 54-55 0.16313908195621707 1104 56-57 0.26287217521663564 1104 58-59 0.2546302585287421 1104 60-61 0.3210007259628682 1104 62-63 0.26330140539217695 1104 64-65 0.1266281162267049 1104 66-67 0.22920212226875947 1104 68-69 0.20507342806273243 1104 70-71 0.22176786340196486 1104 72-73 0.2665200876536318 1104 74-75 0.058732131359523265 1104 76-77 0.16433980902977297 1104 78-79 0.24352940675662893 1104 80-81 0.2872152792874836 1104 82-83 0.29095240237382214 1104 84-85 0.3491046991457232 1104 86-87 0.2473454283318013 1104 88-89 0.4464464492377118 1104 90-91 0.3647044059621578 1104 92-93 0.3416436361458075 1104 94-95 0.35322550131429864 1104 96-97 0.3151578419256147 1104 98-99 0.5323481153608647 1104 100-101 0.4189451178449879 1105 1 0.039173112196991156 1105 2 0.07570245369231543 1105 3 0.147693328348943 1105 4 0.11911527284024714 1105 5 0.13056636709220015 1105 6 0.1442074219995746 1105 7 0.06759453551752159 1105 8 0.10512697207878574 1105 9 0.15965896670231672 1105 10-11 0.09798979750990355 1105 12-13 0.1291722258245187 1105 14-15 0.23735759175841054 1105 16-17 0.17757749900263065 1105 18-19 0.14531731122779235 1105 20-21 0.13679392009701274 1105 22-23 0.14305664760900783 1105 24-25 0.20014212500209538 1105 26-27 0.20790377595964316 1105 28-29 0.23065981939436853 1105 30-31 0.1930124227766541 1105 32-33 0.19434197185815805 1105 34-35 0.24312390437286524 1105 36-37 0.13191890729934386 1105 38-39 0.1725737700262826 1105 40-41 0.1968438375077568 1105 42-43 0.08030559365931111 1105 44-45 0.09601781328949954 1105 46-47 0.10209304787336038 1105 48-49 0.07757448837293879 1105 50-51 0.06996941401121859 1105 52-53 0.08901916039966551 1105 54-55 -0.02468202017301735 1105 56-57 0.06191217098987778 1105 58-59 0.07453647715821177 1105 60-61 0.007232035731981057 1105 62-63 0.030992534467046085 1105 64-65 -0.02961825139626484 1105 66-67 0.10630203244998171 1105 68-69 0.191305002004718 1105 70-71 0.23487770648327455 1105 72-73 0.19177844872511685 1105 74-75 0.13557843558100657 1105 76-77 0.22983539734276448 1105 78-79 0.16924769280302243 1105 80-81 0.2613413954178796 1105 82-83 0.27893438578376717 1105 84-85 0.18899189732821853 1105 86-87 0.16216658329561184 1105 88-89 0.28613683862852923 1105 90-91 0.2923659476750551 1105 92-93 0.2219970994521958 1105 94-95 0.3359956180787087 1105 96-97 0.28336622148919943 1105 98-99 0.4839614191068371 1105 100-101 0.05604212212458748 1106 1 0.08105713005350168 1106 2 0.08193428617449428 1106 3 0.060962219272362006 1106 4 0.04795183184266705 1106 5 0.11499464348078448 1106 6 0.11531479526165356 1106 7 0.1550353627650196 1106 8 0.04005113869222754 1106 9 0.1889201399475624 1106 10-11 0.18091238145182587 1106 12-13 0.16498828005509836 1106 14-15 0.1550316780216079 1106 16-17 0.0915666271341351 1106 18-19 0.07180622390913527 1106 20-21 0.17290121224844768 1106 22-23 0.1703029816611803 1106 24-25 0.1462133026374559 1106 26-27 0.2099728578986273 1106 28-29 0.24170886396162672 1106 30-31 0.26476632394535926 1106 32-33 0.24691586619328376 1106 34-35 0.32480422804491127 1106 36-37 0.30831657050157446 1106 38-39 0.3625023368923763 1106 40-41 0.23646258704518175 1106 42-43 0.24803607717002052 1106 44-45 0.3425660482711024 1106 46-47 0.4568937083168194 1106 48-49 0.3549080163718301 1106 50-51 0.3819530306741896 1106 52-53 0.3815295045420086 1106 54-55 0.342981141499763 1106 56-57 0.39552596973610576 1106 58-59 0.43315706892122563 1106 60-61 0.4188014051068336 1106 62-63 0.4402705280329471 1106 64-65 0.2843829926383066 1106 66-67 0.2576732586503425 1106 68-69 0.2849277279916578 1106 70-71 0.1814018363941443 1106 72-73 0.3173421677294428 1106 74-75 0.19207770507822985 1106 76-77 0.2416765380829169 1106 78-79 0.46168110856504896 1106 80-81 0.39726345112670103 1106 82-83 0.42222382304823114 1106 84-85 0.4965405358352939 1106 86-87 0.5693824653688395 1106 88-89 0.5811167485348747 1106 90-91 0.5589139139776371 1106 92-93 0.5869291135208385 1106 94-95 0.5312172884105628 1106 96-97 0.5200318844115976 1106 98-99 0.6088813223418335 1106 100-101 0.47756985132046736 1107 1 -0.0011719768663169816 1107 2 0.03635273689823748 1107 3 0.09022683588076319 1107 4 6.654726149619705E-4 1107 5 0.035990495716305304 1107 6 0.04390640942087032 1107 7 0.013765903609183283 1107 8 0.10709320745081641 1107 9 9.761193173929428E-4 1107 10-11 0.06438247837254352 1107 12-13 0.07229095222819382 1107 14-15 0.03339806089385888 1107 16-17 0.05143232070594905 1107 18-19 0.04232679277389195 1107 20-21 0.09124988662849631 1107 22-23 0.10442469926032061 1107 24-25 -0.0031634112684670868 1107 26-27 0.10595689782721962 1107 28-29 0.11717512611908631 1107 30-31 0.26243538696526514 1107 32-33 0.22001732444001476 1107 34-35 0.2588225256572301 1107 36-37 0.2811259607373273 1107 38-39 0.2563055857253502 1107 40-41 0.2323061003940765 1107 42-43 0.1622022229986797 1107 44-45 0.1561778131294389 1107 46-47 0.03724854602967298 1107 48-49 0.11926433037384498 1107 50-51 0.05363880636395635 1107 52-53 0.01503449013298308 1107 54-55 0.0144483645908835 1107 56-57 0.012714521068801332 1107 58-59 0.15621952661870608 1107 60-61 0.09301386813729806 1107 62-63 0.06051557608460456 1107 64-65 0.16960250716743985 1107 66-67 0.09297854946734674 1107 68-69 0.29364752810948147 1107 70-71 0.2276865169783946 1107 72-73 0.20545806490157048 1107 74-75 0.3076145270792203 1107 76-77 0.283671532057145 1107 78-79 0.44111187000529384 1107 80-81 0.38898038239900856 1107 82-83 0.5279312606149524 1107 84-85 0.46224609439792275 1107 86-87 0.4076246346068011 1107 88-89 0.6236728270882104 1107 90-91 0.6112006918693851 1107 92-93 0.6652683829122097 1107 94-95 0.6679725274428101 1107 96-97 0.6766658155373726 1107 98-99 0.7756227504447537 1107 100-101 0.6102003773120259 1108 1 0.04399472787542891 1108 2 0.08741664651733316 1108 3 0.0880204600697283 1108 4 0.04441718539997197 1108 5 0.026570237524502716 1108 6 0.14172608456522084 1108 7 0.12041250684482208 1108 8 0.10623141239987888 1108 9 0.13222247111499996 1108 10-11 0.11674288016045153 1108 12-13 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157.0 7 389.0 8 843.0 9 1542.0 10 2607.0 11 4088.0 12 6057.0 13 8579.0 14 10375.0 15 13910.0 16 18662.0 17 26263.0 18 25173.0 19 16831.0 20 15180.0 21 15911.0 22 17087.0 23 18532.0 24 21759.0 25 25568.0 26 30668.0 27 37530.0 28 46150.0 29 56231.0 30 68560.0 31 83601.0 32 104641.0 33 133376.0 34 181956.0 35 261289.0 36 409429.0 37 669125.0 38 559341.0 39 47190.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9810789867255 19.248482507505997 14.755223828650028 23.015214677118475 2 15.66144033364322 20.712503590013025 39.445531845967686 24.18052423037607 3 19.255843043949927 24.504714316631663 30.127729600899443 26.111713038518968 4 13.157108498020218 16.918046978468087 36.15452777206854 33.770316751443154 5 13.897433838271786 37.50665258338018 33.70920145860018 14.88671211974786 6 32.29664192563535 35.78821032916506 17.16240606416153 14.752741681038062 7 28.856899018208004 33.03992775056249 20.104345174818324 17.998828056411185 8 26.68876935034035 35.3389312582264 19.40230196399234 18.569997427440903 9 26.799838568513785 16.711697793724316 20.052519678715953 36.43594395904594 10-11 25.37251064073055 26.67384782700425 26.63595702601279 21.31768450625241 12-13 26.005016490239928 23.93391925975633 27.615605225153367 22.445459024850376 14-15 22.839138467904547 25.293344882860268 26.028580825505333 25.83893582372985 16-17 22.369927719567848 28.72931946276797 26.57530065236484 22.325452165299335 18-19 22.27616694139057 26.830753514809906 29.2723196724547 21.620759871344823 20-21 22.971846811592034 26.031711895159372 29.256513406571866 21.73992788667673 22-23 22.589740117085466 25.993753037048933 29.25685369216676 22.159653153698837 24-25 22.45099075085952 26.155359902514903 29.30834682141508 22.085302525210505 26-27 22.262828181325844 26.468837246971553 29.237549624805588 22.030784946897015 28-29 22.057805675147037 26.492730819121874 29.461722634572745 21.987740871158344 30-31 22.182094988682103 26.31773895194759 29.27686248514653 22.223303574223774 32-33 21.98905097069869 26.499485488180518 29.195398113728892 22.3160654273919 34-35 22.62072377863143 26.41279349027664 28.85480750399975 22.111675227092178 36-37 22.597652470865203 26.263086429186377 28.905073515785062 22.234187584163358 38-39 22.32219517532143 26.54260635551879 29.085964110467966 22.049234358691816 40-41 22.37224589315306 26.310792685188872 29.191124643191774 22.125836778466294 42-43 22.271567925160753 26.36945243681865 28.816054775034754 22.542924862985846 44-45 22.374602863483382 26.714626755052883 28.48386536397892 22.42690501748481 46-47 22.445224985308144 26.927712667799362 28.089858008788234 22.537204338104257 48-49 22.67232743096626 26.87715145567372 27.99104232200001 22.459478791360013 50-51 22.561887741143387 27.08246276935306 27.83730129022686 22.518348199276687 52-53 22.78809259034923 27.023593361436387 27.70766949285196 22.480644555362424 54-55 22.298744890611793 27.039756927193853 27.771319913376896 22.890178268817454 56-57 22.512376187086296 27.41082135414611 27.59836975977198 22.47843269899561 58-59 22.5634249552394 26.90022202073407 27.870535488693175 22.665817535333353 60-61 22.561907678696 26.92632671464788 28.073230905347696 22.438534701308424 62-63 22.48136272647382 26.96924711625252 28.10431938965535 22.445070767618315 64-65 22.28537545842858 26.731888083451654 28.072987869667195 22.909748588452576 66-67 22.26748965872077 27.129524267126914 27.836348490561157 22.766637583591155 68-69 22.273841867277184 26.986578400760063 27.945268921998057 22.794310809964692 70-71 22.199211354105273 26.97789300604211 27.912181135383307 22.91071450446931 72-73 22.364913424538948 26.95749288462821 27.70525346512821 22.97234022570463 74-75 22.263031917427657 27.190401360053468 27.457474509206087 23.08909221331279 76-77 22.321621790771363 26.90723905083691 27.701954318793682 23.06918483959804 78-79 22.225770644786756 27.086273968015878 27.626613464148193 23.061341923049177 80-81 22.136779461659565 27.41231223071451 27.54808749984474 22.902820807781183 82-83 22.319298140543395 27.187321775419676 27.38829444776412 23.10508563627281 84-85 22.13123930652518 27.013622993505994 27.394028260038084 23.461109439930745 86-87 22.131406787273363 26.97012333235287 27.596075942058505 23.30239393831526 88-89 22.011762263228277 26.89473723751412 27.627846826948165 23.46565367230944 90-91 21.92401077754746 27.027073636329487 27.709036953054834 23.33987863306822 92-93 21.945596500230714 26.765810009024378 27.67847298881005 23.610120501934865 94-95 21.729893158812164 26.624749145670336 27.787215978453112 23.85814171706439 96-97 21.861460886893152 26.476380096287212 27.780796867194702 23.881362149624938 98-99 21.639067236350122 26.523509651180042 27.602776458225858 24.234646654243974 100-101 21.63975199241978 26.188956649376145 28.145876042829133 24.025415315374943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1148.0 1 813.5 2 598.0 3 753.0 4 662.0 5 635.0 6 1196.0 7 2548.5 8 4009.5 9 4532.0 10 3986.0 11 3506.0 12 3642.5 13 3804.0 14 4000.5 15 4333.5 16 4601.5 17 4805.0 18 5075.0 19 5373.5 20 5839.0 21 6585.0 22 7687.0 23 9363.0 24 11449.0 25 13834.5 26 17061.5 27 20756.0 28 23889.5 29 27490.5 30 31946.5 31 36450.5 32 41582.5 33 45961.5 34 50586.0 35 55136.5 36 60600.0 37 67801.0 38 73143.0 39 76171.5 40 78728.5 41 84972.0 42 92224.5 43 97123.5 44 101705.0 45 106993.5 46 107507.0 47 109423.5 48 121824.5 49 124024.0 50 115645.0 51 110636.0 52 105824.5 53 104975.0 54 105120.0 55 98396.0 56 86874.5 57 77127.0 58 68485.0 59 58848.0 60 49974.0 61 41725.5 62 34651.0 63 27074.0 64 20339.5 65 15689.5 66 11835.0 67 9884.0 68 7561.0 69 4886.5 70 3598.5 71 2793.0 72 2260.0 73 1937.5 74 1601.5 75 1318.5 76 932.5 77 537.0 78 334.0 79 216.5 80 128.5 81 77.0 82 43.5 83 25.5 84 18.5 85 10.0 86 3.5 87 3.5 88 2.5 89 2.0 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27396393244922596 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002024699289619792 16-17 5.10428392341124E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.507139872352067E-5 26-27 0.0010718996239163605 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003011527514812632 36-37 0.020332064294921442 38-39 0.00649945486247698 40-41 0.017184422542151176 42-43 0.0048660840069853824 44-45 4.9341411259642E-4 46-47 1.7014279744704134E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.29528350554053E-4 60-61 0.00197365645038568 62-63 0.001378156659321035 64-65 1.7014279744704137E-5 66-67 0.0 68-69 4.083427138728993E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22848476269163184 78-79 0.0 80-81 9.698139454481357E-4 82-83 0.0 84-85 0.0 86-87 0.1028513210567365 88-89 0.35253587631026967 90-91 0.3538289615708672 92-93 0.0 94-95 1.7014279744704137E-5 96-97 0.0 98-99 0.0 100-101 2.55214196170562E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2938708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.86447848376436 #Duplication Level Percentage of deduplicated Percentage of total 1 69.73441503009066 31.98332577725487 2 15.52837129168366 14.244013019906587 3 5.926494177759254 8.154466940999821 4 2.9432358577935505 5.399599106896641 5 1.6473922470337334 3.7778393134199435 6 0.9677939094541685 2.6632417762127343 7 0.6492132207178751 2.0843078055093245 8 0.4597835710523203 1.687018696137399 9 0.31760810258769645 1.3110236988662363 >10 1.6037767693289 13.207115849040289 >50 0.12993421047894263 4.120680368598265 >100 0.08424313073444098 7.413645538872073 >500 0.00610149485936923 1.9645582495724936 >1k 0.0014137610039644784 0.9867156423726683 >5k 7.440847389286727E-5 0.24114179535175434 >10k+ 1.4881694778573455E-4 0.7613064209888107 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11907 0.40517805784038424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10401 0.35393104724933544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7066 0.24044580135215882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4028559489408274E-5 3.4028559489408274E-5 12-13 0.0 0.0 0.0 8.507139872352068E-5 1.7014279744704134E-4 14-15 0.0 0.0 0.0 1.8715707719174548E-4 1.8715707719174548E-4 16-17 0.0 0.0 0.0 2.0417135693644962E-4 3.402855948940827E-4 18-19 0.0 0.0 0.0 2.0417135693644962E-4 3.7431415438349096E-4 20-21 0.0 0.0 0.0 2.0417135693644962E-4 3.913284341281951E-4 22-23 0.0 0.0 0.0 3.062570354046744E-4 4.423712733623075E-4 24-25 0.0 0.0 0.0 4.763998328517158E-4 4.423712733623075E-4 26-27 0.0 0.0 0.0 7.656425885116861E-4 5.444569518305324E-4 28-29 0.0 0.0 0.0 0.002041713569364496 5.444569518305324E-4 30-31 0.0 0.0 0.0 0.006176183547327601 5.444569518305324E-4 32-33 0.0 0.0 0.0 0.01464929486019026 5.444569518305324E-4 34-35 0.0 0.0 0.0 0.028720104209060578 6.125140708093488E-4 36-37 0.0 0.0 0.0 0.05204668173904995 6.125140708093488E-4 38-39 0.0 0.0 0.0 0.08758951212573689 6.29528350554053E-4 40-41 0.0 0.0 0.0 0.13014222576724194 6.465426302987571E-4 42-43 0.0 0.0 0.0 0.17257583945053404 6.635569100434612E-4 44-45 0.0 0.0 0.0 0.22695347751460845 7.486283087669819E-4 46-47 0.0 0.0 0.0 0.29424495390491334 8.336997074905026E-4 48-49 0.0 0.0 0.0 0.36948209893599504 8.507139872352068E-4 50-51 0.0 0.0 0.0 0.4517461415016395 8.507139872352068E-4 52-53 0.0 0.0 0.0 0.5358987691189462 8.84742546724615E-4 54-55 0.0 0.0 0.0 0.6326589780270786 8.84742546724615E-4 56-57 0.0 0.0 0.0 0.7473692520658739 9.017568264693192E-4 58-59 0.0 0.0 0.0 0.8771371636787323 9.187711062140233E-4 60-61 0.0 0.0 0.0 1.0261482256828511 9.187711062140233E-4 62-63 0.0 0.0 0.0 1.1791916719864648 9.527996657034316E-4 64-65 0.0 0.0 0.0 1.3376286449691497 9.527996657034316E-4 66-67 0.0 0.0 0.0 1.5255513647494068 9.527996657034316E-4 68-69 0.0 0.0 0.0 1.7392847469023802 9.527996657034316E-4 70-71 0.0 0.0 0.0 1.9840181467502047 9.698139454481357E-4 72-73 0.0 0.0 0.0 2.234638487389696 0.0010548853441716562 74-75 0.0 0.0 0.0 2.493748953621796 0.0011569710226398812 76-77 0.0 0.0 0.0 2.77844889658993 0.0011909995821292895 78-79 0.0 0.0 0.0 3.104357425099738 0.0011909995821292895 80-81 0.0 0.0 0.0 3.4641754131407407 0.0011909995821292895 82-83 0.0 0.0 0.0 3.8349335830575884 0.0012420424213634017 84-85 0.0 0.0 0.0 4.215134678232747 0.001259056701108106 86-87 0.0 0.0 0.0 4.620976292983175 0.0012760709808528102 88-89 0.0 0.0 0.0 5.064283351731442 0.0013100995403422184 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10050 0.0 33.423157 1 GTATCAA 15090 0.0 31.151325 1 ATCAACG 18490 0.0 25.149046 3 TATCAAC 18720 0.0 25.042831 2 GTACATG 13860 0.0 25.024946 1 TCAACGC 18620 0.0 24.973461 4 CAACGCA 18780 0.0 24.710163 5 TACATGG 13890 0.0 24.595894 2 AACGCAG 19275 0.0 24.124817 6 ACATGGG 14280 0.0 23.193142 3 GAGTACT 10560 0.0 21.816238 12-13 ACGCAGA 21510 0.0 21.529886 7 CGCAGAG 21705 0.0 21.33646 8 AGTACTT 11060 0.0 20.6369 12-13 CATGGGG 6425 0.0 20.087603 4 GTACTTT 11405 0.0 19.867014 14-15 GCAGAGT 23175 0.0 19.532642 9 CATGGGA 8005 0.0 18.967976 4 AGAGTAC 19680 0.0 18.637936 10-11 AGTACAT 10680 0.0 18.037947 2 >>END_MODULE