##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 946266 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.068315885808005 33.0 31.0 34.0 30.0 34.0 2 32.08285408119916 33.0 31.0 34.0 30.0 34.0 3 32.12810879816035 34.0 31.0 34.0 30.0 34.0 4 35.59511807462172 37.0 35.0 37.0 33.0 37.0 5 35.667206683955676 37.0 35.0 37.0 35.0 37.0 6 35.81087664567891 37.0 35.0 37.0 35.0 37.0 7 35.77859396829221 37.0 35.0 37.0 35.0 37.0 8 35.7882751784382 37.0 36.0 37.0 35.0 37.0 9 37.384692042195326 39.0 37.0 39.0 35.0 39.0 10-11 37.29989189086367 39.0 37.0 39.0 34.0 39.0 12-13 37.26454664967356 39.0 37.0 39.0 34.0 39.0 14-15 38.40288037401746 40.0 38.0 41.0 34.0 41.0 16-17 38.39616027628595 40.0 38.0 41.0 34.0 41.0 18-19 38.46474088681195 40.0 38.0 41.0 34.0 41.0 20-21 38.46559424094282 40.0 38.0 41.0 34.0 41.0 22-23 38.46111928358411 40.0 38.0 41.0 34.0 41.0 24-25 38.355832820792465 40.0 38.0 41.0 34.0 41.0 26-27 38.2224068073882 40.0 38.0 41.0 34.0 41.0 28-29 38.10022076244946 40.0 38.0 41.0 34.0 41.0 30-31 37.933207998596586 40.0 37.5 41.0 34.0 41.0 32-33 37.63775619117669 40.0 37.0 41.0 33.0 41.0 34-35 37.28153183143006 40.0 36.0 41.0 33.0 41.0 36-37 36.99507221013965 40.0 35.5 41.0 31.5 41.0 38-39 36.39904212980282 39.0 35.0 40.5 30.5 41.0 40-41 36.45283355842861 39.0 35.0 40.5 31.0 41.0 42-43 36.48959964745642 40.0 35.0 41.0 30.5 41.0 44-45 36.24953342928944 40.0 35.0 41.0 30.0 41.0 46-47 35.80251747394496 40.0 35.0 41.0 28.0 41.0 48-49 35.417378939959804 40.0 35.0 41.0 25.0 41.0 50-51 34.220981732409285 38.0 34.0 40.0 23.0 40.5 52-53 34.37513553271491 38.5 34.5 39.5 23.0 40.5 54-55 34.92664747544559 39.0 35.0 40.5 23.0 41.0 56-57 34.80999898548611 39.0 35.0 41.0 22.5 41.0 58-59 34.673040667211964 39.0 35.0 41.0 22.0 41.0 60-61 34.31526441825026 39.0 35.0 40.5 19.5 41.0 62-63 33.98811116535943 38.0 34.0 40.0 17.0 41.0 64-65 33.62254693711917 38.0 34.0 40.0 13.0 41.0 66-67 33.16674011324511 37.0 33.5 40.0 8.0 41.0 68-69 32.71682750938953 37.0 33.0 39.0 6.5 41.0 70-71 32.20273739096618 36.0 32.5 39.0 2.0 40.5 72-73 31.707522514810847 35.5 32.0 38.5 2.0 40.0 74-75 31.2521014175718 35.0 31.0 37.0 2.0 39.5 76-77 30.784286870710773 35.0 31.0 37.0 2.0 39.0 78-79 30.349538079144764 35.0 31.0 36.0 2.0 39.0 80-81 29.84329881872539 35.0 30.0 36.0 2.0 38.5 82-83 29.3860468409517 34.0 30.0 35.5 2.0 37.0 84-85 28.923541583444823 34.0 29.0 35.0 2.0 37.0 86-87 28.56059289882549 34.0 29.0 35.0 2.0 36.0 88-89 28.038734351651648 34.0 28.5 35.0 2.0 36.0 90-91 27.523364994620962 34.0 27.0 35.0 2.0 36.0 92-93 27.062587052689203 34.0 26.0 35.0 2.0 35.0 94-95 26.574092802658026 34.0 25.0 35.0 2.0 35.0 96-97 25.950232809801896 33.0 24.0 35.0 2.0 35.0 98-99 25.257175043803752 33.0 17.5 35.0 2.0 35.0 100-101 22.686822204327324 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3654246558970087 1101 2 0.45412264110677114 1101 3 0.4015998067423965 1101 4 0.35486302402729564 1101 5 0.4479903476216407 1101 6 0.45806192894144004 1101 7 0.3905701729287614 1101 8 0.39653994287243677 1101 9 0.36166087270345315 1101 10-11 0.61207380441423 1101 12-13 0.5940395351710208 1101 14-15 0.6684138905808439 1101 16-17 0.6917974933881439 1101 18-19 0.7151585746482496 1101 20-21 0.5701796827256231 1101 22-23 0.7175350275368046 1101 24-25 0.7964418232065142 1101 26-27 0.761321898026722 1101 28-29 0.8549722152228583 1101 30-31 0.8035926782681315 1101 32-33 0.9263296742348857 1101 34-35 0.7750955761009024 1101 36-37 0.8007925018342448 1101 38-39 1.1241548180545706 1101 40-41 1.023697169159128 1101 42-43 1.023312096481213 1101 44-45 0.9634058746617313 1101 46-47 1.0696308099955658 1101 48-49 0.9294774387621132 1101 50-51 1.0115924895416697 1101 52-53 1.2829161818895543 1101 54-55 1.2263236336882386 1101 56-57 1.042952411178966 1101 58-59 1.0170160805727377 1101 60-61 0.9423909601373595 1101 62-63 1.1688127273286497 1101 64-65 0.8843387720802056 1101 66-67 0.882317207397989 1101 68-69 0.9697559417762847 1101 70-71 1.0137817508113116 1101 72-73 1.1431185891861197 1101 74-75 0.8900051347729239 1101 76-77 0.846820387844037 1101 78-79 0.7702401974524982 1101 80-81 0.8177629352970506 1101 82-83 0.7542715276551704 1101 84-85 0.7945717498018894 1101 86-87 0.9258357232192793 1101 88-89 0.8863202703507476 1101 90-91 1.0075559891825279 1101 92-93 0.9084044223633967 1101 94-95 1.1429168801249645 1101 96-97 0.9907811472404084 1101 98-99 1.0166021012918343 1101 100-101 0.39855235538064804 1102 1 0.2746123694439646 1102 2 0.3814806035225047 1102 3 0.3986693076196701 1102 4 0.30795483767582965 1102 5 0.32194858476265154 1102 6 0.3021423495712341 1102 7 0.2838255520034636 1102 8 0.263366000218447 1102 9 0.3854295147756659 1102 10-11 0.4545598372889188 1102 12-13 0.4304481370733271 1102 14-15 0.6565079262720417 1102 16-17 0.463576397257377 1102 18-19 0.40470314247537686 1102 20-21 0.5744686579310923 1102 22-23 0.6624954636343219 1102 24-25 0.5905262839138814 1102 26-27 0.6140942569703043 1102 28-29 0.4943779775277761 1102 30-31 0.42892165601307397 1102 32-33 0.4372732343512453 1102 34-35 0.41221869457906024 1102 36-37 0.5211534827650794 1102 38-39 0.9385262397001526 1102 40-41 0.8061613567572508 1102 42-43 0.37194409735848666 1102 44-45 0.4120485528868727 1102 46-47 0.5112466390194825 1102 48-49 0.4695968519119873 1102 50-51 0.4704356421873399 1102 52-53 0.47965641644491797 1102 54-55 0.44108694285805683 1102 56-57 0.26336750029152967 1102 58-59 0.3453870924390259 1102 60-61 0.26845191336480667 1102 62-63 0.3883945504425128 1102 64-65 0.2882404769929465 1102 66-67 0.4443150834714942 1102 68-69 0.413511569027186 1102 70-71 0.4431575876845173 1102 72-73 0.519184159645075 1102 74-75 0.3337189183632816 1102 76-77 0.4395372822941255 1102 78-79 0.5498366514186976 1102 80-81 0.608821666481834 1102 82-83 0.34518409460502397 1102 84-85 0.5304358069632116 1102 86-87 0.4078075985078584 1102 88-89 0.40909909341843687 1102 90-91 0.5322567348654168 1102 92-93 0.4414472067848578 1102 94-95 0.5704818965161351 1102 96-97 0.6950604551174031 1102 98-99 0.6257577827952083 1102 100-101 0.31097718108015826 1103 1 0.31113868157947877 1103 2 0.34115471025211264 1103 3 0.27900492041241165 1103 4 0.16947767855336338 1103 5 0.24012814136729332 1103 6 0.2276766340292511 1103 7 0.36093954811565965 1103 8 0.306766849519569 1103 9 0.4381362281886041 1103 10-11 0.41580034918376185 1103 12-13 0.3356567596781588 1103 14-15 0.5970402147567242 1103 16-17 0.4622428556485687 1103 18-19 0.5200949896474611 1103 20-21 0.5246163310311616 1103 22-23 0.5856627840545698 1103 24-25 0.46955438953268214 1103 26-27 0.5561633002916011 1103 28-29 0.47828291134246115 1103 30-31 0.3854788623446481 1103 32-33 0.6221300160322585 1103 34-35 0.6814407856648685 1103 36-37 0.5242349474045298 1103 38-39 0.8818131160307985 1103 40-41 0.7806411019669142 1103 42-43 0.6618483774195809 1103 44-45 0.5356578801570677 1103 46-47 0.3726978852010987 1103 48-49 0.3720270217722117 1103 50-51 0.3190714377996713 1103 52-53 0.34113008116767674 1103 54-55 0.4933105728682463 1103 56-57 0.46806758360172296 1103 58-59 0.41040881672857665 1103 60-61 0.3762843730982155 1103 62-63 0.2023676655494242 1103 64-65 0.2869770837225545 1103 66-67 0.2062214983562498 1103 68-69 3.327934555841239E-4 1103 70-71 -0.14328035200815492 1103 72-73 -0.017202578992339568 1103 74-75 0.001725635247481705 1103 76-77 -0.13178260569995004 1103 78-79 0.15020229059300405 1103 80-81 0.04378186272361617 1103 82-83 0.1017787615956749 1103 84-85 -0.04841526449841638 1103 86-87 0.13702850358052032 1103 88-89 0.17320831076547094 1103 90-91 -0.0290239857677399 1103 92-93 0.02751777167424052 1103 94-95 -0.1338963722517974 1103 96-97 -0.30293633975438894 1103 98-99 -0.09093694320683099 1103 100-101 -0.021694443699995958 1104 1 0.3127564187908405 1104 2 0.3993733829129269 1104 3 0.27609583156741735 1104 4 0.2669080307092173 1104 5 0.2298313859237524 1104 6 0.3399915875973534 1104 7 0.4131796884330754 1104 8 0.26683570268579615 1104 9 0.47058463088597335 1104 10-11 0.3629869055037247 1104 12-13 0.39429973359001735 1104 14-15 0.33361015776696945 1104 16-17 0.3217494741804572 1104 18-19 0.34249795379613346 1104 20-21 0.5418466513998865 1104 22-23 0.4945323830827917 1104 24-25 0.5138073071359415 1104 26-27 0.476417370177856 1104 28-29 0.4843543926221159 1104 30-31 0.6077133831538575 1104 32-33 0.7116178461074085 1104 34-35 0.6827647758562776 1104 36-37 0.5123431598332999 1104 38-39 0.9391838957233674 1104 40-41 0.7104270497906313 1104 42-43 0.39603925338171564 1104 44-45 0.1691126856880345 1104 46-47 0.16648747719074208 1104 48-49 -0.10022807551907675 1104 50-51 -0.050155794677685606 1104 52-53 0.0060080129616082445 1104 54-55 0.1499721025097287 1104 56-57 0.08271619405060449 1104 58-59 -0.18294881104428384 1104 60-61 -0.09327023830428516 1104 62-63 0.10468400980391124 1104 64-65 0.0421297186475158 1104 66-67 0.19277299348600963 1104 68-69 0.1595316161970004 1104 70-71 0.29018452890367286 1104 72-73 0.0938281182807792 1104 74-75 0.04560888570725652 1104 76-77 0.25294439405029223 1104 78-79 0.2686320070065058 1104 80-81 0.3668042499361128 1104 82-83 0.15687903292142735 1104 84-85 0.28032241799369473 1104 86-87 0.17287699255720312 1104 88-89 0.136580044071561 1104 90-91 0.1210402980003984 1104 92-93 0.3401568403117068 1104 94-95 0.1729741860662095 1104 96-97 0.3706092577298854 1104 98-99 0.4284382980346848 1104 100-101 0.2557907696142685 1105 1 0.1977706868601814 1105 2 0.10110152295295904 1105 3 0.27656965924321497 1105 4 0.40053275918998565 1105 5 0.2639469785810178 1105 6 0.2847321003960275 1105 7 0.30254625004131697 1105 8 0.3710751294075436 1105 9 0.45635382889270204 1105 10-11 0.40353643912951753 1105 12-13 0.4323310594556773 1105 14-15 0.4737247282341883 1105 16-17 0.5582054561217618 1105 18-19 0.4790894291570922 1105 20-21 0.519811002522168 1105 22-23 0.5239962124622366 1105 24-25 0.590518144479951 1105 26-27 0.6836131621621746 1105 28-29 0.4850664651124248 1105 30-31 0.7053910951518176 1105 32-33 0.7541319012204895 1105 34-35 0.616990573377457 1105 36-37 0.5846098662518031 1105 38-39 0.9853035664929948 1105 40-41 0.7663147579553709 1105 42-43 0.8294331905170296 1105 44-45 0.7717043633574292 1105 46-47 0.9364574503582546 1105 48-49 0.9259721991944971 1105 50-51 0.9956244770545055 1105 52-53 0.8619710544028933 1105 54-55 1.100248147060178 1105 56-57 1.2179820167006383 1105 58-59 1.4037563297197977 1105 60-61 1.4243505611335152 1105 62-63 1.2380225783119343 1105 64-65 1.1462466955204675 1105 66-67 1.1968111765432141 1105 68-69 1.2075119922777517 1105 70-71 1.256155449726542 1105 72-73 1.249791958308034 1105 74-75 1.3380830115218139 1105 76-77 1.3463190586441875 1105 78-79 1.358180113825366 1105 80-81 1.4949347069402457 1105 82-83 1.444022597383288 1105 84-85 1.4433737085086236 1105 86-87 1.4765898849291084 1105 88-89 1.6284704035417228 1105 90-91 1.5931044297777284 1105 92-93 1.5612773096673003 1105 94-95 1.385685545153052 1105 96-97 1.5603067001252207 1105 98-99 1.6482850227674248 1105 100-101 1.0675307819657291 1106 1 0.1387333236263899 1106 2 0.07481157241699776 1106 3 0.12720677083950704 1106 4 0.18684565314451618 1106 5 0.13222338509892495 1106 6 0.17222300320721473 1106 7 0.2086521598218809 1106 8 0.07594875557784775 1106 9 0.110740832556246 1106 10-11 0.1571370756243553 1106 12-13 0.2239406759552054 1106 14-15 0.12936745233341185 1106 16-17 0.11218663265039908 1106 18-19 0.3873021861912278 1106 20-21 0.17060477079363778 1106 22-23 0.2891407189937141 1106 24-25 0.41543827424595037 1106 26-27 0.4252110602489978 1106 28-29 0.3813998354512762 1106 30-31 0.44955532562215694 1106 32-33 0.5158231765538446 1106 34-35 0.34221291483967065 1106 36-37 0.3637350559050603 1106 38-39 0.8600281837943058 1106 40-41 0.503657021952705 1106 42-43 0.4183179676821567 1106 44-45 0.41182327440851907 1106 46-47 0.40537035171389135 1106 48-49 0.380726790962008 1106 50-51 0.33660314954337167 1106 52-53 0.39340168756723415 1106 54-55 0.3077312787134616 1106 56-57 0.35705182143487235 1106 58-59 0.45441071055587656 1106 60-61 0.5307161262693825 1106 62-63 0.4505063883996385 1106 64-65 0.4987760223901674 1106 66-67 0.43068540713689885 1106 68-69 0.5798281673878449 1106 70-71 0.5534133411523641 1106 72-73 0.46749292908601703 1106 74-75 0.5277874608180966 1106 76-77 0.5342330578300647 1106 78-79 0.40609978719003337 1106 80-81 0.5170078791762407 1106 82-83 0.3401524373961138 1106 84-85 0.21578548119608598 1106 86-87 0.37223001864236593 1106 88-89 0.25291871300650115 1106 90-91 0.1434208269209023 1106 92-93 0.2211793684073875 1106 94-95 0.48915111152243895 1106 96-97 0.861544534562551 1106 98-99 0.8143756369356048 1106 100-101 0.5692940946993268 1107 1 0.008203506567710406 1107 2 0.0789362993378262 1107 3 0.10912820516433186 1107 4 0.047373326133289595 1107 5 0.1312799943392875 1107 6 0.15558038489221815 1107 7 0.07590178171225404 1107 8 0.12408949928036606 1107 9 0.33588555727505565 1107 10-11 0.22148872626843996 1107 12-13 0.3319873123731014 1107 14-15 0.16537449879019306 1107 16-17 0.17567312211259178 1107 18-19 0.27388726230569915 1107 20-21 0.36336418876557985 1107 22-23 0.23685728454140786 1107 24-25 0.15528610323030279 1107 26-27 0.2329801265355229 1107 28-29 0.24159430838673757 1107 30-31 0.1433508508903074 1107 32-33 0.1537372862722819 1107 34-35 0.23691126738271606 1107 36-37 0.031506208313658135 1107 38-39 0.6206845009775819 1107 40-41 0.5193255371553178 1107 42-43 0.2790907600145047 1107 44-45 0.2255713983473413 1107 46-47 0.2825887397209996 1107 48-49 0.06232082001705663 1107 50-51 -0.08101125397057984 1107 52-53 -0.04122620040801195 1107 54-55 -0.14444842911354527 1107 56-57 -0.21779939749784916 1107 58-59 -0.2078142545305468 1107 60-61 -0.22897535945516267 1107 62-63 -0.19876781496250118 1107 64-65 -0.25519890937625433 1107 66-67 -0.2534681778059351 1107 68-69 -0.19038722471618286 1107 70-71 -0.19669183416880998 1107 72-73 -0.2996396381769344 1107 74-75 -0.21093386224687904 1107 76-77 -0.20933038635747536 1107 78-79 -0.15199465619203423 1107 80-81 -0.2397079852026458 1107 82-83 -0.1644115341167307 1107 84-85 -0.04453589749881104 1107 86-87 0.1447476782451922 1107 88-89 0.15650278662343098 1107 90-91 -0.04293419628720585 1107 92-93 -0.17916252382122977 1107 94-95 -0.21206753145515123 1107 96-97 -0.09579600748149986 1107 98-99 0.24361379674414252 1107 100-101 0.3554770128364204 1108 1 0.1689153786109614 1108 2 0.31820464696610884 1108 3 0.27915331519240283 1108 4 0.18040152958496236 1108 5 0.26603238308867816 1108 6 0.20698616184704122 1108 7 0.197949705052487 1108 8 0.25114624135037644 1108 9 0.35609885515031436 1108 10-11 0.4165441936061782 1108 12-13 0.4466423379501663 1108 14-15 0.4912746410986699 1108 16-17 0.5297805622633618 1108 18-19 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-1.3299124970582312 2316 58-59 -1.1067011065605072 2316 60-61 -1.0056968020993224 2316 62-63 -1.4134449819566086 2316 64-65 -1.2570562872355069 2316 66-67 -1.4165392861038022 2316 68-69 -1.5101197294224278 2316 70-71 -1.3190764570713256 2316 72-73 -1.2223335348159345 2316 74-75 -1.1058748709761481 2316 76-77 -1.2441095241724476 2316 78-79 -1.2411757384309006 2316 80-81 -1.3418094207771638 2316 82-83 -1.506000539828154 2316 84-85 -1.1816192433912533 2316 86-87 -1.2013795039568613 2316 88-89 -1.2900360602952041 2316 90-91 -1.3772284155354306 2316 92-93 -1.7705307175840552 2316 94-95 -1.890487696175839 2316 96-97 -1.695270534414167 2316 98-99 -1.8142754693746745 2316 100-101 -1.1165942717456545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3968.0 3 632.0 4 508.0 5 564.0 6 717.0 7 762.0 8 1016.0 9 1324.0 10 1564.0 11 2355.0 12 3931.0 13 5783.0 14 6343.0 15 7532.0 16 10269.0 17 14207.0 18 12216.0 19 6581.0 20 5941.0 21 5846.0 22 6045.0 23 6768.0 24 7602.0 25 8596.0 26 9986.0 27 11604.0 28 13582.0 29 16175.0 30 19297.0 31 23582.0 32 29668.0 33 37769.0 34 51615.0 35 77327.0 36 112739.0 37 187439.0 38 201346.0 39 33063.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05698565845913 21.264188119976723 15.086228368714647 18.592597852849497 2 13.541039114769337 24.940999092619514 38.57955249307289 22.93840929953826 3 22.441183321686466 27.74876649090486 32.388243017173835 17.421807170234842 4 10.499834793364624 18.031351136113326 43.3239575037701 28.14485656675195 5 9.694956686412592 42.30761490889795 35.91739120764361 12.080037197045842 6 24.400475683636525 40.97523609609317 18.291072679296548 16.333215540973754 7 23.043183814270446 37.395003333227514 23.195983196300645 16.36582965620139 8 23.07534376326186 31.415723630529502 21.781356723240016 23.727575882968623 9 23.48357285507303 20.764148257589145 25.787595952454783 29.964682934883037 10-11 22.969735449735452 26.96978835978836 28.768201058201058 21.292275132275133 12-13 27.465003333015904 26.579055962924166 27.718206731634027 18.237733972425907 14-15 22.6337661952842 28.10748917139619 31.24950394253247 18.00924069078714 16-17 17.289042063305235 31.23708913358002 31.72858151732602 19.745287285788724 18-19 16.577690644950486 32.89239536784957 33.10569441247516 17.424219574724777 20-21 19.232741852248818 28.765934923848462 31.371945006814606 20.629378217088114 22-23 17.792049136855077 28.899978510036235 32.33895495258975 20.969017400518936 24-25 20.420053949454815 29.320496292629468 31.998697589216462 18.260752168699256 26-27 19.92053741522165 26.70905165226809 35.984715150559154 17.38569578195111 28-29 16.861463119767397 31.407758793629387 33.984777981799006 17.746000104804217 30-31 17.93196890067375 29.258188977628173 33.01794721159291 19.791894910105164 32-33 18.341394682156313 29.914114611949007 33.18216319388206 18.562327512012626 34-35 20.39386329217025 28.196944372563408 32.6646806290274 18.744511706238946 36-37 19.685607880507636 27.295893312664077 35.10271363613439 17.915785170693898 38-39 18.84031281908672 29.501126157203476 33.965315207261746 17.69324581644805 40-41 17.931478404654495 30.260653334090403 33.97180195674404 17.83606630451106 42-43 17.039536399516784 31.02642412353055 31.333743091957906 20.600296384994767 44-45 18.653856871773776 28.930691755463844 31.47440916148038 20.941042211281996 46-47 20.36655834539164 30.519209597951484 30.917372559719137 18.19685949693774 48-49 19.61131783184429 29.552883316247687 31.6420751871237 19.193723664784326 50-51 17.01601558560137 30.592688144644576 33.90473460417562 18.486561665578435 52-53 18.491756213034087 32.18992164726031 30.500870379542818 18.81745176016279 54-55 17.05892094510457 29.530643074084573 32.07944661891674 21.330989361894115 56-57 19.63117267901751 29.642018044480857 30.38352480744701 20.34328446905463 58-59 21.424558257487934 28.862173152984433 30.306043138368 19.407225451159633 60-61 18.968275727481906 29.687593631494014 33.11546748531195 18.228663155712123 62-63 18.59383634837643 29.527302610099902 32.55662595469286 19.3222350868308 64-65 17.876168205694448 31.893734091035768 31.324820240316598 18.905277462953183 66-67 18.393768491958703 29.430783467093935 30.234143435749516 21.941304605197843 68-69 17.25911180750502 30.1013670830694 31.201851166509943 21.43766994291564 70-71 19.96819708252034 30.07281753142463 29.788237168000638 20.170748218054392 72-73 19.147948947863263 27.480260325337657 32.88807911053209 20.48371161626699 74-75 17.388335818918442 32.11326410505397 30.579492622086118 19.91890745394146 76-77 18.600852970135428 28.815475542793145 30.72789084954658 21.855780637524845 78-79 18.34435623996289 28.99427785366972 31.882470447252974 20.77889545911441 80-81 19.535097974803524 29.078480144731948 30.746994524789166 20.639427355675362 82-83 18.421144817029482 28.31905753171054 32.514805681730124 20.744991969529856 84-85 18.046292851194423 29.068458410582735 31.57242725640363 21.31282148181921 86-87 18.046160770775742 29.61018543288196 31.357296934646268 20.986356861696027 88-89 18.387629472311914 28.768557941331185 30.17956926519868 22.664243321158217 90-91 18.53467613187798 27.14622392328443 31.563039379802486 22.756060565035103 92-93 19.647185313989144 28.975934799633645 31.397002800748634 19.979877085628576 94-95 18.723134493186993 27.957435692684086 31.37004527846682 21.9493845356621 96-97 16.784249306343835 27.706161620559193 32.88583263693404 22.623756436162925 98-99 18.55861174480628 28.687818311262692 30.02102918230406 22.73254076162697 100-101 18.06278123070831 26.577904323302583 31.929654701638672 23.429659744350438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4341.0 1 2556.0 2 952.5 3 1120.0 4 888.0 5 831.0 6 1524.0 7 2922.0 8 4138.5 9 4206.5 10 3309.5 11 2609.5 12 2523.0 13 2527.0 14 2539.5 15 2579.0 16 2685.5 17 2757.5 18 2871.0 19 3116.5 20 3466.0 21 3880.0 22 4409.0 23 5023.5 24 5706.0 25 7016.5 26 9305.5 27 11857.0 28 12861.5 29 13918.5 30 16770.5 31 19631.0 32 23226.5 33 27916.0 34 32872.0 35 35341.5 36 37356.5 37 40164.0 38 38142.0 39 34715.5 40 32313.5 41 30013.0 42 27762.0 43 25928.5 44 24889.0 45 23854.5 46 27309.5 47 44815.5 48 90361.0 49 88201.5 50 38134.5 51 16614.0 52 9467.5 53 6814.5 54 5480.5 55 4495.5 56 3596.5 57 2870.0 58 2334.0 59 1885.5 60 1484.0 61 1152.5 62 921.0 63 738.5 64 516.0 65 375.0 66 289.0 67 245.5 68 202.0 69 150.5 70 121.5 71 114.5 72 104.0 73 75.0 74 55.5 75 47.0 76 36.0 77 24.5 78 14.5 79 12.5 80 10.0 81 5.0 82 6.5 83 5.5 84 1.5 85 1.0 86 2.0 87 2.5 88 2.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2941033493753342 2 0.18905889041770496 3 0.1908554254300588 4 0.21072298909608927 5 0.22245330594145835 6 0.11624638315230601 7 0.13061866325113658 8 0.14340576539789024 9 0.14763290660342862 10-11 0.13378901915529037 12-13 0.12427795144282898 14-15 0.1389672671320749 16-17 0.11450268740502142 18-19 0.14250749789171333 20-21 0.16945552307702064 22-23 0.17320711089693597 24-25 0.19693194091302021 26-27 0.17653598459629746 28-29 0.17410537840311285 30-31 0.16390740024475148 32-33 0.1727843967763821 34-35 0.354023075963841 36-37 0.4701109413209394 38-39 0.4358182582910091 40-41 0.4266770654340323 42-43 0.4489223960281781 44-45 0.45214559119740116 46-47 0.45600285754745495 48-49 0.4694768701401086 50-51 0.46192085523520876 52-53 0.4687371204291394 54-55 0.45177571634191654 56-57 0.41510526638387096 58-59 0.4051186452857864 60-61 0.3600995914468025 62-63 0.4655667645249856 64-65 0.4632418368619395 66-67 0.4725415475141239 68-69 0.4850116140704622 70-71 0.47856522373201615 72-73 0.46968822720038556 74-75 0.47581758194841617 76-77 0.45056041324532425 78-79 0.43719207918280906 80-81 0.4343915981341399 82-83 0.44691450395554744 84-85 0.44823548558227816 86-87 0.4495564672090089 88-89 0.45626705387280103 90-91 0.4757119034182777 92-93 0.4812071869854777 94-95 0.4795691697683315 96-97 0.47734992063542386 98-99 0.4733869757552316 100-101 0.4557386612221088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 946266.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.749190741903725 #Duplication Level Percentage of deduplicated Percentage of total 1 72.11198995313087 20.010493639876735 2 12.549691780506183 6.964875819387349 3 5.064436420881827 4.216020367298821 4 2.655139075673604 2.947118426285951 5 1.5851433689339394 2.199322284890587 6 1.0400225890331318 1.7315871119381352 7 0.7041782630535548 1.3678263856442883 8 0.5285150652551266 1.1732692284587363 9 0.41187984140740286 1.0286399053763169 >10 2.728585876372416 15.068942875418145 >50 0.35523305155268703 6.901471341245438 >100 0.23275205131157603 12.12485020385159 >500 0.014117747374636577 2.6895642044993058 >1k 0.012209943675361363 7.31503864894292 >5k 0.004960289618115553 8.807629255620611 >10k+ 0.0011446822195651277 5.453350301265127 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24873 2.628542080133916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15425 1.6300913273857458 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11207 1.1843392872617213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9211 0.9734049411053552 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8381 0.8856917610904333 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7107 0.7510573136940353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6849 0.7237922529183126 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6288 0.6645065975106366 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6199 0.6551012083283135 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6033 0.6375585723253293 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6001 0.6341768593608985 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5981 0.6320632887581292 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5433 0.5741514542422532 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5385 0.5690788847956071 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5162 0.5455125725747306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5155 0.5447728228637614 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4376 0.46244924788590097 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4131 0.4365580080019783 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3835 0.40527716308099415 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3819 0.4035863065987788 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3407 0.36004675218173327 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3384 0.3576161459885487 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3243 0.34271547323902585 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3153 0.33320440552656444 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2883 0.3046712023891802 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2633 0.27825156985456523 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2472 0.26123732650227316 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2388 0.2523603299706425 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2227 0.23534608661835046 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2195 0.23196437365391975 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2001 0.2114627388070585 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1968 0.20797534731248932 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1876 0.198252922539751 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.18187275036828968 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1649 0.17426389619832056 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1568 0.1657039352571053 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1415 0.1495351201459209 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1403 0.1482669777842594 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1381 0.14594205012121328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1262 0.13336630503473654 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.12332684467158282 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1147 0.12121327406881363 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1110 0.1173031684536906 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1105 0.11677477580299832 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1083 0.1144498481399522 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1073 0.1133930628385676 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1007 0.10641827984942923 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1006 0.10631260131929077 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 980 0.10356495953569081 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.10197978158361391 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 947 0.10007756804112163 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0567853013846001E-4 0.0 10-11 0.0 0.0 0.0 1.0567853013846001E-4 0.0 12-13 0.0 0.0 0.0 2.1135706027692003E-4 0.0 14-15 0.0 0.0 0.0 3.1703559041538E-4 0.0 16-17 0.0 0.0 0.0 3.6987485548461003E-4 0.0 18-19 0.0 0.0 0.0 5.283926506923001E-4 0.0 20-21 0.0 0.0 0.0 9.5110677124614E-4 0.0 22-23 0.0 0.0 0.0 0.0022720883979768904 0.0 24-25 0.0 0.0 0.0 0.00427998047060763 0.0 26-27 0.0 0.0 0.0 0.007344657844622971 0.0 28-29 0.0 0.0 0.0 0.01838806424409204 0.0 30-31 0.0 0.0 0.0 0.04744966003216855 0.0 32-33 0.0 0.0 0.0 0.10599556572887539 0.0 34-35 0.0 0.0 0.0 0.19814724400961253 0.0 36-37 0.0 0.0 0.0 0.3279204790196414 0.0 38-39 0.0 0.0 0.0 0.5055132489173235 0.0 40-41 0.0 0.0 0.0 0.7004901370227822 0.0 42-43 0.0 0.0 0.0 0.8995884878036409 0.0 44-45 0.0 0.0 0.0 1.1371009842898299 0.0 46-47 0.0 0.0 0.0 1.4341104932439714 0.0 48-49 0.0 0.0 0.0 1.721608934485652 0.0 50-51 0.0 1.0567853013846001E-4 0.0 2.032092456032448 0.0 52-53 0.0 1.0567853013846001E-4 0.0 2.322391378322797 0.0 54-55 0.0 1.0567853013846001E-4 0.0 2.6426501639074003 0.0 56-57 0.0 1.0567853013846001E-4 0.0 3.07772867248744 0.0 58-59 0.0 1.0567853013846001E-4 0.0 3.514603716079834 0.0 60-61 0.0 1.0567853013846001E-4 0.0 4.0236043564917265 0.0 62-63 0.0 1.0567853013846001E-4 0.0 4.501958223163466 0.0 64-65 0.0 1.0567853013846001E-4 0.0 4.965147220760336 0.0 66-67 0.0 1.0567853013846001E-4 0.0 5.517106183673512 0.0 68-69 0.0 1.0567853013846001E-4 0.0 6.135219906453365 0.0 70-71 0.0 1.0567853013846001E-4 0.0 6.800413414409902 0.0 72-73 0.0 1.0567853013846001E-4 0.0 7.420006636611692 1.0567853013846001E-4 74-75 0.0 1.0567853013846001E-4 0.0 8.132332769009983 1.0567853013846001E-4 76-77 0.0 1.0567853013846001E-4 0.0 8.796416652400065 1.0567853013846001E-4 78-79 0.0 1.0567853013846001E-4 0.0 9.708211010434699 1.0567853013846001E-4 80-81 0.0 1.0567853013846001E-4 0.0 10.654826444149954 1.0567853013846001E-4 82-83 0.0 1.0567853013846001E-4 0.0 11.487414743845811 1.0567853013846001E-4 84-85 0.0 1.0567853013846001E-4 0.0 12.426579841186305 1.0567853013846001E-4 86-87 0.0 1.0567853013846001E-4 0.0 13.387356197940115 1.0567853013846001E-4 88-89 0.0 1.0567853013846001E-4 0.0 14.381844005808091 1.0567853013846001E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGAC 25 0.0050672637 56.868065 7 GTAAGCG 85 0.0 36.355915 94-95 CATTCCG 280 0.0 35.542538 9 TTAGGCA 370 0.0 30.760666 4 GCGTACG 50 4.9247574E-5 28.525412 94-95 CGTTTCG 50 4.9265407E-5 28.523895 46-47 GTATTGA 170 3.6779966E-9 27.91345 1 TAGGCAT 370 0.0 26.915583 5 GGCATTC 370 0.0 26.897055 7 CATGGGG 1775 0.0 26.71701 4 GTACATG 5570 0.0 26.665613 1 GCTTTGC 255 0.0 26.052555 1 CCTTTAG 365 0.0 26.001572 1 ACATGGG 5645 0.0 25.45452 3 TACATGG 5800 0.0 25.428377 2 ACTGTCC 225 1.05501385E-10 25.274693 8 ATGGGAC 340 0.0 25.106133 5 CTTTAGG 480 0.0 24.69932 2 GATTGGC 365 0.0 24.668795 9 CATGGGA 2795 0.0 24.093103 4 >>END_MODULE