##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939339_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 946266 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18354669828568 33.0 31.0 34.0 30.0 34.0 2 32.3717760122418 34.0 31.0 34.0 30.0 34.0 3 32.45630298457305 34.0 31.0 34.0 30.0 34.0 4 35.97033075266363 37.0 35.0 37.0 35.0 37.0 5 35.94230903361211 37.0 35.0 37.0 35.0 37.0 6 36.019704818729615 37.0 35.0 37.0 35.0 37.0 7 35.97668942982206 37.0 35.0 37.0 35.0 37.0 8 35.97575945875684 37.0 35.0 37.0 35.0 37.0 9 37.59976475959191 39.0 37.0 39.0 35.0 39.0 10-11 37.52913821272243 39.0 37.0 39.0 34.5 39.0 12-13 37.49412374533165 39.0 37.0 39.0 35.0 39.0 14-15 38.58633988751578 40.0 38.0 41.0 34.0 41.0 16-17 38.650634176859356 40.0 38.0 41.0 34.5 41.0 18-19 38.88323209330146 40.0 38.0 41.0 35.0 41.0 20-21 38.884363381966594 40.0 38.0 41.0 35.0 41.0 22-23 38.81341028843898 40.0 38.0 41.0 34.5 41.0 24-25 38.70157387034935 40.0 38.0 41.0 34.0 41.0 26-27 38.46004717489585 40.0 38.0 41.0 34.0 41.0 28-29 38.32035178269113 40.0 38.0 41.0 34.0 41.0 30-31 38.13390156678989 40.0 37.0 41.0 34.0 41.0 32-33 37.94228314237223 40.0 37.0 41.0 33.0 41.0 34-35 37.74678261714993 40.0 36.5 41.0 33.0 41.0 36-37 37.505273887046556 40.0 36.0 41.0 33.0 41.0 38-39 37.31257331448028 40.0 36.0 41.0 33.0 41.0 40-41 37.19488283421363 40.0 36.0 41.0 32.5 41.0 42-43 36.93658706959776 40.0 36.0 41.0 31.5 41.0 44-45 36.59395719596815 40.0 36.0 41.0 31.0 41.0 46-47 36.11195742000663 40.0 35.0 41.0 29.5 41.0 48-49 35.634080691898475 39.0 35.0 41.0 27.0 41.0 50-51 35.346981187108064 39.0 35.0 41.0 26.5 41.0 52-53 35.16205379882612 39.0 35.0 41.0 26.0 41.0 54-55 34.98533340519474 39.0 35.0 40.0 24.0 41.0 56-57 34.668979441298745 38.5 34.5 40.0 23.5 41.0 58-59 34.41325800567705 38.0 34.0 40.0 22.5 41.0 60-61 33.97018227432878 38.0 34.0 40.0 22.0 41.0 62-63 33.70868339346441 38.0 34.0 40.0 21.0 41.0 64-65 33.43068650886748 37.5 33.5 40.0 19.5 41.0 66-67 32.951004791464555 37.0 33.0 39.0 16.0 40.5 68-69 32.46076737407875 36.0 32.5 39.0 12.0 40.0 70-71 31.90887815899546 35.5 31.5 38.5 8.0 40.0 72-73 31.362596246721324 35.0 31.0 37.5 4.5 39.5 74-75 30.865778755656443 35.0 31.0 37.0 2.0 39.0 76-77 28.90288248758806 33.0 28.5 35.0 2.0 37.5 78-79 29.818786155267127 34.0 29.5 36.0 2.0 38.0 80-81 29.77357688007389 34.0 30.0 36.0 2.0 37.0 82-83 29.39431407236443 34.0 29.5 35.0 2.0 37.0 84-85 28.945252709069123 34.0 29.0 35.0 2.0 36.5 86-87 28.446852153622764 34.0 29.0 35.0 2.0 36.0 88-89 27.80535864122773 34.0 27.5 35.0 2.0 35.5 90-91 27.281474765023788 33.0 26.0 35.0 2.0 35.0 92-93 26.992563930226808 33.0 26.0 35.0 2.0 35.0 94-95 26.510217000293785 33.0 25.0 35.0 2.0 35.0 96-97 25.773190096653586 32.0 24.5 34.0 2.0 35.0 98-99 24.934069278617216 32.0 11.0 34.0 2.0 35.0 100-101 20.881824983672665 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30376821776079765 1101 2 0.2958089871707017 1101 3 0.26033894429350113 1101 4 0.13538193087927652 1101 5 0.2955858024562019 1101 6 0.2245768264998489 1101 7 0.2502987215740191 1101 8 0.2521165333343873 1101 9 0.21982156115906548 1101 10-11 0.29578191260363695 1101 12-13 0.19172660914321682 1101 14-15 0.3249880650483732 1101 16-17 0.2640561112086317 1101 18-19 0.3045698620455539 1101 20-21 0.3473885792062319 1101 22-23 0.33975642254985416 1101 24-25 0.2638995157757691 1101 26-27 0.34852800068407674 1101 28-29 0.3740321903682755 1101 30-31 0.4120702147936939 1101 32-33 0.42735567981019074 1101 34-35 0.5779556695783583 1101 36-37 0.33366061091025756 1101 38-39 0.279197962401895 1101 40-41 0.21033833688940007 1101 42-43 0.22087828360611894 1101 44-45 0.293164771015455 1101 46-47 0.1894519252037341 1101 48-49 0.40376990960370307 1101 50-51 0.6179880323607065 1101 52-53 0.5037912275391676 1101 54-55 0.1941712298415652 1101 56-57 0.46838966670910054 1101 58-59 0.365291255083541 1101 60-61 0.4089010880640842 1101 62-63 0.44717631077228504 1101 64-65 0.6009000521200463 1101 66-67 0.4155542815707065 1101 68-69 0.6623394179206912 1101 70-71 0.5291111519037557 1101 72-73 0.691241810769327 1101 74-75 0.7434853069882692 1101 76-77 0.7868920249290774 1101 78-79 0.8134891615391417 1101 80-81 1.0541764757682195 1101 82-83 0.8837057794884551 1101 84-85 0.9061900041461364 1101 86-87 0.8963707051698862 1101 88-89 1.0034906673861848 1101 90-91 0.7902962721809423 1101 92-93 0.8913499734565562 1101 94-95 0.8856600117681879 1101 96-97 0.8244999591658662 1101 98-99 0.9792618492272815 1101 100-101 0.9491819343202792 1102 1 0.24306893804891416 1102 2 0.3771602776869116 1102 3 0.30689795251217333 1102 4 0.1944218930179744 1102 5 0.08194361481191237 1102 6 0.2477264709730207 1102 7 0.21453970642950537 1102 8 0.3497680939586374 1102 9 0.3794782122810574 1102 10-11 0.3719135671885496 1102 12-13 0.44317237975914736 1102 14-15 0.443308229652672 1102 16-17 0.38178108581386283 1102 18-19 0.46526971891137947 1102 20-21 0.5724468717540176 1102 22-23 0.5382901629691048 1102 24-25 0.4634209108722658 1102 26-27 0.4883716785398349 1102 28-29 0.42238903310536813 1102 30-31 0.46384597317396725 1102 32-33 0.32387775017679843 1102 34-35 0.3716801305165731 1102 36-37 0.5084134447360924 1102 38-39 0.5540184675270226 1102 40-41 0.5690698102479743 1102 42-43 0.501788484224825 1102 44-45 0.6284601872588667 1102 46-47 0.7097352789298412 1102 48-49 0.54514015962679 1102 50-51 0.4708144994860177 1102 52-53 0.4698235539312634 1102 54-55 0.4529021260461903 1102 56-57 0.460846264732929 1102 58-59 0.37595489553972783 1102 60-61 0.667287856232889 1102 62-63 0.7599757093778834 1102 64-65 0.5439566016629485 1102 66-67 0.4569650766579727 1102 68-69 0.44980969449285624 1102 70-71 0.10727583123702544 1102 72-73 0.3242274569508723 1102 74-75 0.2761875705860177 1102 76-77 0.5167955247905631 1102 78-79 0.5042363738856217 1102 80-81 0.7554004749832934 1102 82-83 0.5109458582122528 1102 84-85 0.392892377403129 1102 86-87 0.3331824490982278 1102 88-89 0.45649157928940554 1102 90-91 0.46536437634568273 1102 92-93 0.25669130229578485 1102 94-95 0.4445369279192626 1102 96-97 0.5374546121809196 1102 98-99 0.4522243515358966 1102 100-101 0.7255326188248503 1103 1 0.13890227138224986 1103 2 0.1795256507314349 1103 3 0.18870740359057692 1103 4 0.0875389669789115 1103 5 0.041072444766555805 1103 6 0.15300162602254375 1103 7 0.17027104263240744 1103 8 0.09145732380219584 1103 9 0.1712880683395568 1103 10-11 0.11188669386631744 1103 12-13 0.12302114861964952 1103 14-15 0.336883075760241 1103 16-17 0.18270154772258707 1103 18-19 0.12274623006567964 1103 20-21 0.24810698931395336 1103 22-23 0.26136911094651083 1103 24-25 0.10265234542747947 1103 26-27 0.17307659268405473 1103 28-29 0.14956261914628755 1103 30-31 0.03319519908344404 1103 32-33 0.1768294765491092 1103 34-35 0.2064243855846115 1103 36-37 0.3935748293051944 1103 38-39 0.15320677170178953 1103 40-41 -0.20250261176443018 1103 42-43 -0.42059635679155605 1103 44-45 -0.047471122331060656 1103 46-47 -0.031063717876598673 1103 48-49 -0.33119989059074584 1103 50-51 -0.3927180551284337 1103 52-53 -0.47577821756739525 1103 54-55 -0.3587430766752746 1103 56-57 -0.2542965196788103 1103 58-59 -0.3642583264819379 1103 60-61 -0.2945217973355625 1103 62-63 -0.4354973363038752 1103 64-65 -0.4018122241261892 1103 66-67 -0.443161045715442 1103 68-69 -0.47863466124166365 1103 70-71 -0.4533605371328733 1103 72-73 -0.4991455355049226 1103 74-75 -0.6392865743274356 1103 76-77 -0.28162852408475203 1103 78-79 -0.4248942784794636 1103 80-81 -0.2685230011344508 1103 82-83 -0.2784753384460821 1103 84-85 -0.18241077912746206 1103 86-87 -0.03220361264387961 1103 88-89 0.23428912395292656 1103 90-91 0.2419368562123907 1103 92-93 0.13073381363522785 1103 94-95 0.15217167058333914 1103 96-97 0.39592780828385443 1103 98-99 0.20370180571529062 1103 100-101 0.5542932929431501 1104 1 0.09566327767155514 1104 2 0.11507301353596944 1104 3 -0.0380766483586541 1104 4 -0.010630719463883054 1104 5 0.130791582881578 1104 6 0.07845941727200767 1104 7 0.09495969917261249 1104 8 0.02016903735486153 1104 9 0.15671424276001034 1104 10-11 0.012617939467212125 1104 12-13 0.11443507932374075 1104 14-15 0.0984130010605071 1104 16-17 0.12878761701850294 1104 18-19 2.8423269947808194E-4 1104 20-21 0.12542219831424717 1104 22-23 0.05944854468172878 1104 24-25 0.04629690869520431 1104 26-27 0.15071066620311768 1104 28-29 0.06743620291668861 1104 30-31 0.19136094051794572 1104 32-33 0.10707804045256353 1104 34-35 0.20627964067622884 1104 36-37 0.13269909422810144 1104 38-39 0.24233486810757654 1104 40-41 0.340681747838687 1104 42-43 0.23000646317983353 1104 44-45 0.26841619233869807 1104 46-47 -0.23700592208611937 1104 48-49 -0.468046977528509 1104 50-51 0.03452776145989134 1104 52-53 0.08506076365550541 1104 54-55 0.13761275739597778 1104 56-57 0.08589162032073716 1104 58-59 0.045029187992561504 1104 60-61 0.243020348975989 1104 62-63 0.22814742563332402 1104 64-65 0.1282046060170856 1104 66-67 0.11330578783358902 1104 68-69 0.12622025327369357 1104 70-71 0.3139045503952218 1104 72-73 0.3879860745125576 1104 74-75 0.4155085974365207 1104 76-77 0.4800574986657402 1104 78-79 0.263931130779671 1104 80-81 0.33451195901812625 1104 82-83 0.32892254544011834 1104 84-85 0.281725831684696 1104 86-87 0.15060353037543805 1104 88-89 0.3826753601312092 1104 90-91 0.6507326637186885 1104 92-93 0.7027181528037652 1104 94-95 0.5592638872806575 1104 96-97 -0.011200594640559558 1104 98-99 0.18731370204387687 1104 100-101 0.7341388416114967 1105 1 0.10466600351166733 1105 2 0.15555333637765756 1105 3 -0.0038439297590571186 1105 4 0.12700024863526949 1105 5 0.17003942137554873 1105 6 0.14917420495461187 1105 7 0.22301828343940144 1105 8 0.15387362443267705 1105 9 0.23311762450539675 1105 10-11 0.2601143724498769 1105 12-13 0.11638496006823118 1105 14-15 0.3041686763448084 1105 16-17 0.3253422509466972 1105 18-19 0.2563521550982131 1105 20-21 0.21796367154564678 1105 22-23 0.2736798790656465 1105 24-25 0.3896382988736562 1105 26-27 0.41450224591264373 1105 28-29 0.38549569224758073 1105 30-31 0.24603879093746883 1105 32-33 0.24841977136103566 1105 34-35 0.46952019606614925 1105 36-37 0.4409958700703527 1105 38-39 0.4891471791088975 1105 40-41 0.6029759788536353 1105 42-43 0.45336563520590545 1105 44-45 0.4741053187921267 1105 46-47 0.5445142661672051 1105 48-49 0.4428641025856308 1105 50-51 0.5471800251849217 1105 52-53 0.3653471365845036 1105 54-55 0.43345159209932405 1105 56-57 0.5486761831215361 1105 58-59 0.643255262356881 1105 60-61 0.563728481775513 1105 62-63 0.5214401020675794 1105 64-65 0.700198462229018 1105 66-67 0.6169646425548336 1105 68-69 0.7043379329022983 1105 70-71 0.6845855312717575 1105 72-73 0.8107282057788012 1105 74-75 0.8732381110444294 1105 76-77 0.8553742711006826 1105 78-79 0.7269326427691034 1105 80-81 0.9727165780393783 1105 82-83 1.0605923354652482 1105 84-85 0.9782804656128867 1105 86-87 1.0175324881675074 1105 88-89 1.1411563431372969 1105 90-91 1.2261843971826352 1105 92-93 1.421156704275294 1105 94-95 1.4869732232830408 1105 96-97 1.4870864384528453 1105 98-99 1.5139633144634885 1105 100-101 1.1602882730182884 1106 1 0.11157986701066136 1106 2 -0.017972919034399126 1106 3 0.07952825936379071 1106 4 0.067685344047824 1106 5 -0.030616809736216055 1106 6 0.10287043902682314 1106 7 0.017099868262206996 1106 8 -0.012561141014039379 1106 9 -0.016697964684055933 1106 10-11 0.002332729651762122 1106 12-13 0.050047642474581266 1106 14-15 -0.05673314802702123 1106 16-17 0.027806201408651532 1106 18-19 0.11321791142923843 1106 20-21 0.014736173981845013 1106 22-23 0.14656963963997072 1106 24-25 0.11619715614339299 1106 26-27 0.007877642180019961 1106 28-29 0.1558931314660228 1106 30-31 0.18491246644845916 1106 32-33 0.04503685063917828 1106 34-35 0.14292001597264914 1106 36-37 0.09667991699333811 1106 38-39 0.08099671468970371 1106 40-41 0.40312955173599363 1106 42-43 0.33036732512436373 1106 44-45 0.23994676250056557 1106 46-47 0.04375626989243386 1106 48-49 0.06404975924847633 1106 50-51 0.011722575358234621 1106 52-53 0.11735468570931573 1106 54-55 0.18361750015296252 1106 56-57 0.23903563001245232 1106 58-59 0.16581999116814217 1106 60-61 0.22182035413116807 1106 62-63 0.3592788567086913 1106 64-65 0.5614573898092274 1106 66-67 0.6169640257962712 1106 68-69 0.3848835175277401 1106 70-71 0.18189029634841347 1106 72-73 0.3832497614905961 1106 74-75 0.2750927040454485 1106 76-77 0.444338610120532 1106 78-79 0.5806609482866314 1106 80-81 0.4937326260972057 1106 82-83 0.4789766747318005 1106 84-85 0.6260996073316711 1106 86-87 0.6629888278287872 1106 88-89 0.7105571005168159 1106 90-91 0.8440161207761179 1106 92-93 0.9552332316778767 1106 94-95 1.1312133150567156 1106 96-97 0.7959554265987414 1106 98-99 0.8935856115190823 1106 100-101 0.7674889292493958 1107 1 -0.07460073321301763 1107 2 -0.06927066080725552 1107 3 -0.11408357126627777 1107 4 -0.003894140100548782 1107 5 -0.10045554091041708 1107 6 0.03724335652648847 1107 7 0.03679401958718387 1107 8 -0.003525914524928453 1107 9 -0.014300083256920004 1107 10-11 0.17057615691853556 1107 12-13 -0.011154543317772436 1107 14-15 0.15726646951726053 1107 16-17 0.18114023011601432 1107 18-19 0.15478099634020026 1107 20-21 0.07396871285035189 1107 22-23 0.14907993986770407 1107 24-25 0.2562663458588119 1107 26-27 0.33209809892485964 1107 28-29 0.15015281889539978 1107 30-31 0.12626172384694456 1107 32-33 0.07666651761297061 1107 34-35 -0.09294853515762469 1107 36-37 -0.033125552123365765 1107 38-39 0.07763631971725715 1107 40-41 0.13122440075290598 1107 42-43 0.1785648577055312 1107 44-45 -0.08089301752944777 1107 46-47 -0.2626504427848104 1107 48-49 -0.2878581732307097 1107 50-51 -0.2872911524839665 1107 52-53 -0.3581159955833826 1107 54-55 -0.23483367010357625 1107 56-57 -0.19399405666359826 1107 58-59 -0.3340050063690754 1107 60-61 -0.6839483528787866 1107 62-63 -0.666716542233182 1107 64-65 -0.6568257317247728 1107 66-67 -0.44225692350517676 1107 68-69 -0.3475012097407699 1107 70-71 -0.4063410777686052 1107 72-73 -0.3356365970094828 1107 74-75 -0.36829747611437114 1107 76-77 -0.43078380468166344 1107 78-79 -0.3144905563289164 1107 80-81 -0.37267443426705427 1107 82-83 -0.2981733348295954 1107 84-85 -0.35149949795375335 1107 86-87 -0.1582536896569735 1107 88-89 0.2175485068528289 1107 90-91 0.06046287540760176 1107 92-93 0.02916713637686641 1107 94-95 -0.15030538181236253 1107 96-97 -0.20849033132996553 1107 98-99 0.04869110555335254 1107 100-101 -0.15539351197701023 1108 1 0.029403134940103826 1108 2 0.15350418960400702 1108 3 0.1439694949593644 1108 4 0.07734586671267607 1108 5 0.01687037846276951 1108 6 0.09049749528549711 1108 7 0.08470087621826394 1108 8 0.11897146183428475 1108 9 0.21968471885737983 1108 10-11 0.11953027205104405 1108 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-1.1587359287492163 2316 48-49 -1.0112824162361989 2316 50-51 -1.2445714617211578 2316 52-53 -1.3617193177642264 2316 54-55 -1.2589955665927803 2316 56-57 -1.3575670725757547 2316 58-59 -1.2075466656144513 2316 60-61 -1.211439554652955 2316 62-63 -1.2403384884290531 2316 64-65 -1.2323128195919466 2316 66-67 -1.2936105213013462 2316 68-69 -1.3255921888305409 2316 70-71 -1.2829718208202223 2316 72-73 -1.0858907476804092 2316 74-75 -1.1590271191708261 2316 76-77 -1.4217604283002778 2316 78-79 -1.3464171782577559 2316 80-81 -1.3219858754771856 2316 82-83 -1.4167322251707724 2316 84-85 -1.4414679220734499 2316 86-87 -1.444867598937286 2316 88-89 -1.7830182514268174 2316 90-91 -1.604953817519018 2316 92-93 -1.49124027194393 2316 94-95 -1.2808127788984685 2316 96-97 -1.2379229428114584 2316 98-99 -1.1112420525918445 2316 100-101 -0.481908848245979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 25.0 6 88.0 7 206.0 8 364.0 9 724.0 10 1282.0 11 2008.0 12 3183.0 13 4579.0 14 5282.0 15 7430.0 16 9942.0 17 14904.0 18 13442.0 19 7648.0 20 6094.0 21 6023.0 22 6560.0 23 7148.0 24 8133.0 25 9249.0 26 10602.0 27 12623.0 28 15065.0 29 18352.0 30 22250.0 31 27126.0 32 33185.0 33 41486.0 34 56303.0 35 83402.0 36 124053.0 37 207144.0 38 169010.0 39 11341.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04977291636857 21.875811486139156 15.591768814554548 18.482646782937724 2 13.786081292152524 25.525380812583354 38.19634225471485 22.492195640549276 3 22.37647765004766 27.766611079759812 32.57614666489127 17.280764605301258 4 10.675750793117368 18.621719474228176 42.731007982956164 27.971521749698287 5 9.512547211883339 42.57650597189374 35.87944615995925 12.031500656263672 6 24.19869254522513 41.609230385536414 17.87277573113691 16.31930133810155 7 23.01128858058939 37.3913888906502 22.893774055075422 16.70354847368499 8 23.147402527407728 31.92812591808223 21.34051101910034 23.583960535409705 9 23.151735347143404 20.843399213329022 25.413572927696865 30.59129251183071 10-11 23.025555182158083 27.325033341576262 28.527602175286866 21.121809300978793 12-13 27.509865090788427 26.702269763470316 27.589388184717617 18.198476961023644 14-15 22.410701972141773 28.401525803548832 31.124627545097493 18.063144679211902 16-17 17.193100186788477 31.432247564748494 31.578349445050634 19.79630280341239 18-19 16.54292584903497 32.87465304399241 33.08833514484043 17.494085962132193 20-21 19.12443224209683 28.682051347084226 31.59412892357963 20.59938748723932 22-23 17.73423117812539 28.90878463349629 32.39902944837921 20.957954739999114 24-25 20.3557456360051 29.408115688400514 32.22254630304798 18.01359237254641 26-27 19.834222089771796 26.741952051537304 36.18586105809571 17.237964800595183 28-29 16.726058000604482 31.506785618420192 34.15181354925571 17.615342831719623 30-31 17.99985416362841 29.236969308841275 33.03362902186066 19.729547505669654 32-33 18.31070756003069 29.835109789424962 33.301788292087004 18.55239435845735 34-35 20.289945437079325 28.091449695481202 33.07122770270342 18.547377164736055 36-37 19.622701331642357 27.294546607482562 35.24635383639823 17.836398224476856 38-39 18.601607302982796 29.4891853946685 34.29688024277049 17.612327059578217 40-41 17.920580661900036 30.14313108698145 34.05512715981994 17.881161091298576 42-43 17.07890317787587 30.91446228894565 31.58388647529593 20.42274805788255 44-45 18.735541688022785 28.729887820936217 31.60892148522333 20.92564900581767 46-47 20.455243174866197 30.560673639002246 30.904816681611415 18.07926650452015 48-49 19.708632800817533 29.54427046180607 31.597346615053105 19.149750122323287 50-51 16.962954352110668 30.598193952011325 33.90181397388681 18.537037721991197 52-53 18.435004993315825 32.25729578923452 30.422450370667836 18.88524884678182 54-55 17.09482323152264 29.533714621470075 31.831113027415125 21.540349119592165 56-57 19.661782321774957 29.611748213614835 30.32831019561862 20.398159268991588 58-59 21.39620681437612 28.931851483144772 30.158219370261225 19.513722332217878 60-61 18.83850288741537 30.043228008325396 32.80948236824599 18.308786736013246 62-63 18.528076630223136 29.70182037994903 32.30760939003454 19.46249359979329 64-65 17.753570349140727 32.13694669363583 30.98219739481288 19.12728556241057 66-67 18.39632785721042 29.591354168703194 29.89005658595766 22.122261388128734 68-69 17.208631131974776 30.31453666001232 30.85287276393423 21.62395944407867 70-71 19.865238738367434 30.23320081245654 29.571177660404157 20.330382788771868 72-73 19.189847252252537 27.737496644706667 32.38439297195504 20.688263131085762 74-75 17.300377114034063 32.4082934440704 29.959245053317492 20.332084388578046 76-77 18.54710036678469 28.901616522474306 30.415954180519094 22.135328930221913 78-79 18.323893583665033 29.28657896391548 31.31455317197253 21.074974280446956 80-81 19.50786487287645 29.19992243183592 30.3579496007723 20.93426309451533 82-83 18.35456415003815 28.700016697207765 31.981916289922708 20.963502862831383 84-85 17.777612214747226 29.46333272039786 30.997996335068574 21.76105872978634 86-87 17.87983136825513 30.02269228938223 30.541652781524565 21.555823560838082 88-89 18.087874345816303 29.260759160693183 29.45778327453547 23.193583218955048 90-91 18.387500655687848 27.540373151291426 30.75332307899356 23.318803114027165 92-93 19.372618270127003 29.562987574318427 30.51451705968512 20.549877095869448 94-95 18.531522848755 28.510059539283876 30.555256133053497 22.40316147890762 96-97 16.4873302010217 28.577852316367704 31.853622554334613 23.08119492827598 98-99 18.12259977638423 29.293243126140005 29.010658736549765 23.573498360926 100-101 17.64230950103829 27.65573068854919 30.80051571177207 23.901444098640447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3108.0 1 1911.0 2 845.0 3 944.5 4 751.0 5 749.0 6 1424.0 7 2763.0 8 3885.0 9 3924.5 10 3073.0 11 2376.0 12 2253.0 13 2283.0 14 2341.0 15 2320.5 16 2331.5 17 2510.5 18 2735.0 19 2966.5 20 3318.5 21 3752.0 22 4254.5 23 4938.0 24 5530.0 25 6575.5 26 9144.5 27 11834.0 28 12764.5 29 13870.5 30 16844.5 31 19915.0 32 23517.5 33 28117.0 34 33052.5 35 35806.0 36 37710.5 37 40104.5 38 38348.0 39 35156.0 40 32771.0 41 30423.0 42 27882.5 43 25942.0 44 25056.0 45 24392.5 46 26786.5 47 45259.0 48 90038.5 49 86974.0 50 41342.0 51 20172.0 52 9962.5 53 6267.5 54 5031.5 55 4162.0 56 3393.5 57 2719.0 58 2176.5 59 1759.0 60 1352.0 61 1007.5 62 770.0 63 589.0 64 417.0 65 294.5 66 206.0 67 169.0 68 128.0 69 67.5 70 49.5 71 53.0 72 50.0 73 32.5 74 17.5 75 18.0 76 16.5 77 8.5 78 3.5 79 3.0 80 3.0 81 2.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2938919923150573 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.00110962456645383 16-17 8.982675061769102E-4 18-19 5.283926506923001E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020078920726307402 36-37 0.006974782989138361 38-39 0.00237776692811535 40-41 0.0037515878199153307 42-43 5.283926506923001E-4 44-45 0.0011624638315230601 46-47 2.6419632534615005E-4 48-49 3.1703559041538E-4 50-51 1.0567853013846001E-4 52-53 1.0567853013846001E-4 54-55 0.0 56-57 0.00121530309659229 58-59 0.0017436957472845903 60-61 4.7555338562307E-4 62-63 7.9258897603845E-4 64-65 0.0 66-67 6.8691044589999E-4 68-69 0.0017965350123538204 70-71 0.0 72-73 0.0 74-75 5.283926506923001E-5 76-77 0.2523603299706425 78-79 1.5851779520769E-4 80-81 1.5851779520769E-4 82-83 0.0 84-85 0.0 86-87 0.10684099396998308 88-89 0.3686067131229485 90-91 0.2748170176250652 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.0567853013846001E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 946266.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.50861215474279 #Duplication Level Percentage of deduplicated Percentage of total 1 70.66038331953759 18.731086961230787 2 13.163321047437046 6.978827446297424 3 5.304230764426836 4.218233883403375 4 2.81791845229298 2.9879642934211073 5 1.6326993961197487 2.164029752851059 6 1.1193315767302834 1.780315598405988 7 0.7716728656086075 1.431918369333027 8 0.549275358508174 1.1648421955880386 9 0.43847546030661494 1.0461038324976333 >10 2.881562824618157 15.190159627126132 >50 0.36941924238732704 6.814177100197558 >100 0.2566407673021205 12.758095098677044 >500 0.015541909821091148 2.8337824047529327 >1k 0.01315084677169251 7.529991046068557 >5k 0.005180636607030383 8.973239329898364 >10k+ 0.001195531524699319 5.397233060250963 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26295 2.778816949990806 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14294 1.5105689097991475 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10502 1.109835923514107 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9007 0.9518465209571093 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8088 0.8547279517598646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7101 0.7504232425132046 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6859 0.7248490382196972 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6686 0.7065666525057436 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6470 0.6837400899958362 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6299 0.6656690613421596 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6279 0.6635554907393904 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6011 0.635233644662283 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5926 0.626250969600514 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5534 0.5848249857862378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5465 0.5775331672066839 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5217 0.5513248917323459 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4092 0.4324365453265784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4047 0.42768101147034765 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3962 0.41869833640857856 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3596 0.38001999437790224 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3549 0.3750531034613946 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3503 0.3701918910750254 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3342 0.35317764772273336 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3184 0.33648043996085664 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3093 0.3268636937182568 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2549 0.2693745733229346 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2468 0.2608146123817193 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2410 0.2546852576336886 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2350 0.248344545825381 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2303 0.2433776549088734 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2189 0.23133030247308897 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2154 0.22763155391824283 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1924 0.20332549198639704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1793 0.1894816045382588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.17743425210247435 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1601 0.16919132675167448 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1500 0.15851779520769 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1461 0.15439633253229007 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1437 0.15186004780896706 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1255 0.13262655532376733 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1189 0.12565177233462896 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1155 0.1220587023099213 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1150 0.12153030965922902 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1122 0.11857131081535212 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1088 0.1149782407906445 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1048 0.11075109958510608 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1036 0.10948295722344456 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1029 0.10874320751247536 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1022 0.10800345780150612 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 989 0.10451606630693694 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 967 0.10219113864389083 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 964 0.10187410305347545 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 957 0.10113435334250621 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0567853013846001E-4 0.0 10-11 0.0 0.0 0.0 1.0567853013846001E-4 0.0 12-13 0.0 0.0 0.0 2.1135706027692003E-4 0.0 14-15 0.0 0.0 0.0 3.1703559041538E-4 0.0 16-17 0.0 0.0 0.0 3.6987485548461003E-4 0.0 18-19 0.0 0.0 0.0 5.283926506923001E-4 0.0 20-21 0.0 0.0 0.0 9.5110677124614E-4 0.0 22-23 0.0 0.0 0.0 0.0022720883979768904 0.0 24-25 0.0 0.0 0.0 0.004227141205538401 0.0 26-27 0.0 0.0 0.0 0.007238979314484511 0.0 28-29 0.0 0.0 0.0 0.01838806424409204 0.0 30-31 0.0 0.0 0.0 0.04750249929723778 0.0 32-33 0.0 0.0 0.0 0.10694667250012153 0.0 34-35 0.0 0.0 0.0 0.19983810049182787 0.0 36-37 0.0 0.0 0.0 0.33188342389983366 0.0 38-39 0.0 0.0 0.0 0.5110613717495927 0.0 40-41 0.0 0.0 0.0 0.7075705985420591 0.0 42-43 0.0 0.0 0.0 0.9087825199256868 0.0 44-45 0.0 0.0 0.0 1.1471404446529834 0.0 46-47 0.0 0.0 0.0 1.4448368640530251 0.0 48-49 0.0 0.0 0.0 1.7338676439817133 0.0 50-51 0.0 0.0 0.0 2.0445625225887856 0.0 52-53 0.0 0.0 0.0 2.3372920510723203 0.0 54-55 0.0 0.0 0.0 2.6611967459467003 0.0 56-57 0.0 0.0 0.0 3.0987586999849936 0.0 58-59 0.0 0.0 0.0 3.537324600059603 0.0 60-61 0.0 0.0 0.0 4.050393863881826 0.0 62-63 0.0 0.0 0.0 4.53043858703578 0.0 64-65 0.0 0.0 0.0 4.997484850982705 0.0 66-67 0.0 0.0 0.0 5.548545546389704 0.0 68-69 0.0 0.0 0.0 6.166183715783934 0.0 70-71 0.0 0.0 0.0 6.830426116969225 0.0 72-73 0.0 0.0 0.0 7.451868713448439 0.0 74-75 0.0 0.0 0.0 8.165885702328943 0.0 76-77 0.0 0.0 0.0 8.830762174695064 0.0 78-79 0.0 0.0 0.0 9.743824675091359 0.0 80-81 0.0 0.0 0.0 10.69281787573473 0.0 82-83 0.0 0.0 0.0 11.526410121466903 0.0 84-85 0.0 0.0 0.0 12.46467695130122 0.0 86-87 0.0 0.0 0.0 13.426351575561206 0.0 88-89 0.0 0.0 0.0 14.416136688838023 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTACA 50 0.0016241834 37.959377 7 GTAAGCG 220 0.0 37.7437 94-95 CATGGGG 1740 0.0 26.99697 4 GACATAT 285 0.0 26.735636 1 CTCCGAC 160 0.0 26.690187 94-95 ATAGACC 125 7.4507334E-6 26.571566 3 GTACATG 5645 0.0 24.633965 1 TAGACCC 135 1.3535735E-5 24.603298 5 ATGGGGA 915 0.0 23.854254 5 TACATGG 5760 0.0 23.806986 2 GCAAGCG 50 0.0016643684 23.724611 94-95 TATACGT 100 0.0016637556 23.724611 4 ATGGGCG 60 1.7238411E-4 23.72461 18-19 CATTCCG 220 2.1955202E-9 23.72461 9 CTGACCG 180 1.9705294E-7 23.72461 4 GAGTACT 6260 0.0 23.497217 12-13 ACATGGG 5675 0.0 23.327457 3 GGGACCA 125 2.2718866E-4 22.775627 9 CATATAG 315 0.0 22.594868 3 TCTAGAC 190 3.3197102E-7 22.475946 3 >>END_MODULE