##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939338_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962667 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04348959712964 33.0 31.0 34.0 30.0 34.0 2 32.07519630360239 33.0 31.0 34.0 30.0 34.0 3 32.12177835118478 34.0 31.0 34.0 30.0 34.0 4 35.603518142826125 37.0 35.0 37.0 33.0 37.0 5 35.684437089876354 37.0 35.0 37.0 35.0 37.0 6 35.82837679072826 37.0 35.0 37.0 35.0 37.0 7 35.78570367531036 37.0 35.0 37.0 35.0 37.0 8 35.790460252610714 37.0 35.0 37.0 35.0 37.0 9 37.391821886488266 39.0 37.0 39.0 35.0 39.0 10-11 37.3176586503952 39.0 37.0 39.0 34.0 39.0 12-13 37.27827847012519 39.0 37.0 39.0 34.0 39.0 14-15 38.423417962805416 40.0 38.0 41.0 34.0 41.0 16-17 38.4254939662417 40.0 38.0 41.0 34.0 41.0 18-19 38.50636928449817 40.0 38.0 41.0 34.0 41.0 20-21 38.441470415003316 40.0 38.0 41.0 34.0 41.0 22-23 38.37179730893445 40.0 38.0 41.0 34.0 41.0 24-25 38.366363446550054 40.0 38.0 41.0 34.0 41.0 26-27 38.23642443337104 40.0 38.0 41.0 34.0 41.0 28-29 38.136058470893886 40.0 38.0 41.0 34.0 41.0 30-31 37.972352848908294 40.0 37.5 41.0 34.0 41.0 32-33 37.6969268708702 40.0 37.0 41.0 33.0 41.0 34-35 37.3201828877483 40.0 36.0 41.0 33.0 41.0 36-37 37.02759728961313 40.0 35.5 41.0 31.5 41.0 38-39 36.69805914194628 39.0 35.0 41.0 30.5 41.0 40-41 36.65003370843708 39.0 35.0 41.0 31.0 41.0 42-43 36.43899292278638 39.0 35.0 41.0 30.5 41.0 44-45 36.19956589350211 40.0 35.0 41.0 30.0 41.0 46-47 35.81416834689462 40.0 35.0 41.0 28.0 41.0 48-49 35.47847594235598 40.0 35.0 41.0 26.0 41.0 50-51 34.277744536792056 38.0 34.0 40.0 23.5 40.5 52-53 34.44854866740005 38.5 34.5 39.5 23.0 40.5 54-55 35.016161351744685 39.0 35.0 41.0 23.0 41.0 56-57 34.89032084822685 39.0 35.0 41.0 22.5 41.0 58-59 34.759507181611085 39.0 35.0 41.0 22.0 41.0 60-61 34.41853101851419 39.0 35.0 41.0 21.0 41.0 62-63 34.127381015449785 38.5 34.5 40.0 19.0 41.0 64-65 33.77241663004965 38.0 34.0 40.0 16.0 41.0 66-67 33.33292509247747 37.5 34.0 40.0 11.5 41.0 68-69 32.86863889590066 37.0 33.0 39.0 7.5 41.0 70-71 32.36091711879601 36.0 33.0 39.0 4.5 40.5 72-73 31.876268740904177 36.0 32.0 38.5 2.0 40.0 74-75 31.435443928170386 35.0 32.0 37.0 2.0 40.0 76-77 30.973643534056947 35.0 31.5 37.0 2.0 39.0 78-79 30.505269215627003 35.0 31.0 36.5 2.0 39.0 80-81 29.939800574861295 35.0 30.5 36.0 2.0 38.5 82-83 29.473648727960967 34.5 30.0 35.5 2.0 37.0 84-85 29.027904768731034 34.0 29.0 35.0 2.0 37.0 86-87 28.778231205598615 34.0 29.0 35.0 2.0 36.0 88-89 28.287994186982623 34.0 29.0 35.0 2.0 36.0 90-91 27.80328504041377 34.0 28.5 35.0 2.0 36.0 92-93 27.38096766586992 34.0 27.0 35.0 2.0 35.0 94-95 26.90125557435749 34.0 25.5 35.0 2.0 35.0 96-97 26.315630430875892 34.0 25.0 35.0 2.0 35.0 98-99 25.676697653498042 33.5 22.0 35.0 2.0 35.0 100-101 23.35815240368684 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15046489332259938 1101 2 0.0024215639273847955 1101 3 0.24950933515012963 1101 4 0.3627673151775639 1101 5 0.2189269490742376 1101 6 0.16750340104624684 1101 7 0.2051822400088028 1101 8 0.17785383019573686 1101 9 0.2510380289430145 1101 10-11 0.4070468042886901 1101 12-13 0.2724274275634855 1101 14-15 0.2816716198504565 1101 16-17 0.33050315930926644 1101 18-19 0.37887696693631057 1101 20-21 0.38527781298547836 1101 22-23 0.4887524878322367 1101 24-25 0.43267006030966826 1101 26-27 0.4535378883957364 1101 28-29 0.34475388171910737 1101 30-31 0.4911466335472383 1101 32-33 0.5546475902355468 1101 34-35 0.5884730925480781 1101 36-37 0.4354617518220394 1101 38-39 0.6885985158396934 1101 40-41 0.568852944519783 1101 42-43 0.3718805493709638 1101 44-45 0.5316983625669636 1101 46-47 0.7406194343030847 1101 48-49 0.7305616331941138 1101 50-51 0.7011615330145631 1101 52-53 0.7006533222832587 1101 54-55 0.6219813345499219 1101 56-57 0.8280889776587017 1101 58-59 0.8602679061865288 1101 60-61 0.8704011307691246 1101 62-63 0.7760412764821325 1101 64-65 0.7383753484281144 1101 66-67 0.700160120446192 1101 68-69 0.7840879080233947 1101 70-71 0.7673400470475968 1101 72-73 0.7878623534458882 1101 74-75 0.6160034288223493 1101 76-77 0.5174189808014411 1101 78-79 0.7473055587394732 1101 80-81 0.6824550486562657 1101 82-83 0.40933370734199315 1101 84-85 0.2473883545052118 1101 86-87 0.25469938481837673 1101 88-89 0.15465957701303878 1101 90-91 0.2560069245962602 1101 92-93 0.2830695351208732 1101 94-95 0.2853526294296529 1101 96-97 0.11737768388331205 1101 98-99 0.40417023898600846 1101 100-101 0.35916963731686025 1102 1 0.14033172921651982 1102 2 0.3144484453254961 1102 3 0.3267100083548584 1102 4 0.3151341327937729 1102 5 0.2598938839602525 1102 6 0.19394905848096045 1102 7 0.18827023769264883 1102 8 0.2837066463526483 1102 9 0.2572392914212074 1102 10-11 0.29620855912460087 1102 12-13 0.26533713712331775 1102 14-15 0.3888433557799047 1102 16-17 0.4128406009850778 1102 18-19 0.39567637854018045 1102 20-21 0.3914622599676818 1102 22-23 0.3229524284662233 1102 24-25 0.30075176314914387 1102 26-27 0.3141600909917557 1102 28-29 0.2635196828020199 1102 30-31 0.21754242938079926 1102 32-33 0.14551121948748857 1102 34-35 0.10006701147983676 1102 36-37 0.261365804064134 1102 38-39 0.17663026934499726 1102 40-41 0.43610776362159953 1102 42-43 0.4066973993752683 1102 44-45 0.639110633199941 1102 46-47 0.6643172934711146 1102 48-49 0.5973741316405139 1102 50-51 0.5728980473523251 1102 52-53 0.5474049340120004 1102 54-55 0.5474082653709758 1102 56-57 0.60278399573901 1102 58-59 0.5732273896144875 1102 60-61 0.5305541079161387 1102 62-63 0.4582876220361882 1102 64-65 0.5132363281427743 1102 66-67 0.42022378537622984 1102 68-69 0.449974238194919 1102 70-71 0.49764508014487774 1102 72-73 0.4709363138461242 1102 74-75 0.5672070527810753 1102 76-77 0.5451828269001737 1102 78-79 0.48074558517635424 1102 80-81 0.544015994593714 1102 82-83 0.515296920885632 1102 84-85 0.5580224741701301 1102 86-87 0.449600487768695 1102 88-89 0.38321068076757214 1102 90-91 0.3770821512764577 1102 92-93 0.5230697983199413 1102 94-95 0.49477062318013765 1102 96-97 0.44204616272430286 1102 98-99 0.548198617694375 1102 100-101 0.6369046046289988 1103 1 0.23178461347010426 1103 2 0.27738516995794527 1103 3 0.27872939245752093 1103 4 0.19323784270483202 1103 5 0.15772075293975973 1103 6 0.23997389780819844 1103 7 0.13587637544051745 1103 8 0.2567640667949931 1103 9 0.3260746326759616 1103 10-11 0.26492922764459337 1103 12-13 0.1773919705377338 1103 14-15 0.4207553168904141 1103 16-17 0.5082064166569253 1103 18-19 0.49155353537305757 1103 20-21 0.3920307996682908 1103 22-23 0.3530299014760985 1103 24-25 0.5082156901665726 1103 26-27 0.6150299222767401 1103 28-29 0.5144434736731327 1103 30-31 0.41406903083652935 1103 32-33 0.4894419031271937 1103 34-35 0.438921497016878 1103 36-37 0.5823143901575847 1103 38-39 0.6246416208431143 1103 40-41 0.5466718514840707 1103 42-43 0.6156732974268024 1103 44-45 0.7893257134915288 1103 46-47 0.7378463606663104 1103 48-49 0.4713519661727119 1103 50-51 0.399288764373928 1103 52-53 0.6243238761008882 1103 54-55 0.8098909852097691 1103 56-57 0.7692531092167769 1103 58-59 0.8622543666509941 1103 60-61 0.7549563831775572 1103 62-63 0.6863512929695403 1103 64-65 0.8023783913974682 1103 66-67 0.8765523558400119 1103 68-69 0.7310995471014934 1103 70-71 0.6938808649295183 1103 72-73 0.6760206437893572 1103 74-75 0.6162551450374956 1103 76-77 0.5982995959958721 1103 78-79 0.625298010049864 1103 80-81 0.5635317228138206 1103 82-83 0.5995717329918762 1103 84-85 0.5681796943486717 1103 86-87 0.4920349127441952 1103 88-89 0.5949727679216075 1103 90-91 0.40485721078361436 1103 92-93 0.3769282188408454 1103 94-95 0.5652281977139673 1103 96-97 0.5976151641480634 1103 98-99 0.507000508140564 1103 100-101 0.4226657517973962 1104 1 0.1823485359392052 1104 2 0.32405228686211274 1104 3 0.24375918096507831 1104 4 0.3388141930531674 1104 5 0.3357830631613439 1104 6 0.2795126957005536 1104 7 0.2889928796906247 1104 8 0.3424124816279388 1104 9 0.39748362444853313 1104 10-11 0.47008987635738464 1104 12-13 0.35434921254171314 1104 14-15 0.44147617361291935 1104 16-17 0.44228767393190793 1104 18-19 0.3740441962554897 1104 20-21 0.3920036529954771 1104 22-23 0.5121928305093988 1104 24-25 0.5486980004676028 1104 26-27 0.3964753700445556 1104 28-29 0.3365135862457507 1104 30-31 0.5197927412702654 1104 32-33 0.6034473351102108 1104 34-35 0.6796164783254 1104 36-37 0.7029542041300445 1104 38-39 0.6435582169946414 1104 40-41 0.6083060547027372 1104 42-43 0.5941135423030275 1104 44-45 0.6849878075991072 1104 46-47 0.6459687775941774 1104 48-49 0.5081972844310414 1104 50-51 0.6794583185196146 1104 52-53 0.7384107481023392 1104 54-55 0.7025762149037362 1104 56-57 0.7234911021109696 1104 58-59 0.8353910786195016 1104 60-61 0.6521909979662652 1104 62-63 0.6983130439696694 1104 64-65 0.537022313125 1104 66-67 0.6065806810756911 1104 68-69 0.648124086369485 1104 70-71 0.648811429037373 1104 72-73 0.536327176073371 1104 74-75 0.49443676819664617 1104 76-77 0.6074665639243939 1104 78-79 0.6918417883635115 1104 80-81 0.6248130192859449 1104 82-83 0.5643989146243023 1104 84-85 0.4822862973464588 1104 86-87 0.7023368764418088 1104 88-89 0.695665074289689 1104 90-91 0.47307348898801393 1104 92-93 0.5807752334351619 1104 94-95 0.5832648443980339 1104 96-97 0.6171514989764475 1104 98-99 0.5053697214299859 1104 100-101 0.31940113265197567 1105 1 0.16317983822495563 1105 2 0.20008153394728367 1105 3 0.2213446819331324 1105 4 0.23166916786043146 1105 5 0.1772166921428706 1105 6 0.22033139192946294 1105 7 0.22251013745994896 1105 8 0.22270531412883088 1105 9 0.3047826088601653 1105 10-11 0.3242141622617183 1105 12-13 0.223225555648618 1105 14-15 0.24711880481730475 1105 16-17 0.12784082279832631 1105 18-19 0.19630525271022492 1105 20-21 0.1847289507425316 1105 22-23 0.08318843776356033 1105 24-25 0.02692801192413441 1105 26-27 0.19025655421310006 1105 28-29 0.24284063850792847 1105 30-31 0.21514012467392263 1105 32-33 -0.023587102932189907 1105 34-35 0.2961398415077099 1105 36-37 0.2303872312240216 1105 38-39 0.44468217095670326 1105 40-41 0.27606412690599313 1105 42-43 0.24255761132118892 1105 44-45 0.2756474427725877 1105 46-47 0.28221170767577064 1105 48-49 0.5791941832893954 1105 50-51 0.4160989335298666 1105 52-53 0.11617914022303921 1105 54-55 0.17437080850692865 1105 56-57 0.10012223674650045 1105 58-59 0.10816432071391802 1105 60-61 0.24211958517086174 1105 62-63 0.04800906492464918 1105 64-65 -0.23838257249699524 1105 66-67 -0.10288781088600985 1105 68-69 -0.2572336169516163 1105 70-71 -0.28018004766661164 1105 72-73 -0.31459716046185093 1105 74-75 -0.337954339883499 1105 76-77 -0.479486167237237 1105 78-79 -0.313463475827664 1105 80-81 -0.24634494261431428 1105 82-83 -0.11350600005878064 1105 84-85 -0.25423613588083427 1105 86-87 -0.45513744323797667 1105 88-89 -0.3237296541569883 1105 90-91 -0.2427232176796359 1105 92-93 -0.06573078350293571 1105 94-95 -0.021846550004926968 1105 96-97 -0.07725495077821165 1105 98-99 -0.2040561475936009 1105 100-101 -0.11270117273233637 1106 1 0.13585793091564113 1106 2 0.13402193882827618 1106 3 0.07476861708924076 1106 4 0.23808201666740558 1106 5 0.13049766883314362 1106 6 0.07883078285018286 1106 7 0.015656735650779297 1106 8 0.07172371926304777 1106 9 0.16678159352316868 1106 10-11 0.16751668720569768 1106 12-13 0.19416552907284768 1106 14-15 0.3893065487409544 1106 16-17 0.246423210757861 1106 18-19 0.1960606376225158 1106 20-21 0.1705547970923007 1106 22-23 0.12257699838200864 1106 24-25 0.12129842308645067 1106 26-27 0.20105403627568563 1106 28-29 0.19079048135195364 1106 30-31 0.19823525535861108 1106 32-33 0.2941322485796931 1106 34-35 0.4056333653539923 1106 36-37 0.32660946464581997 1106 38-39 0.6032290098291142 1106 40-41 0.7158611578442731 1106 42-43 0.8054900668264438 1106 44-45 0.9189740604588721 1106 46-47 0.6786571677303712 1106 48-49 0.8495639208382997 1106 50-51 0.8388386716810956 1106 52-53 0.804199638721542 1106 54-55 1.0172664611190854 1106 56-57 0.9319936159207813 1106 58-59 0.8456843416598474 1106 60-61 0.7159617506658762 1106 62-63 0.797538580377811 1106 64-65 0.6955037466359713 1106 66-67 0.7625615986574203 1106 68-69 0.7913599692162023 1106 70-71 1.0454198431785144 1106 72-73 0.7947279600325885 1106 74-75 0.822803253939913 1106 76-77 0.8661617124170569 1106 78-79 0.8761931165489472 1106 80-81 0.8156625236773607 1106 82-83 0.8042351086744688 1106 84-85 0.848306754731972 1106 86-87 1.091027201154379 1106 88-89 0.8243884691167231 1106 90-91 0.8000985587175897 1106 92-93 0.8699538867522421 1106 94-95 0.9319924932673622 1106 96-97 0.9287018568350973 1106 98-99 0.8518291807867904 1106 100-101 0.581616627747799 1107 1 0.048566297341821496 1107 2 0.09911263497544098 1107 3 0.0971836405723252 1107 4 0.08286844033968066 1107 5 0.22740183746562792 1107 6 0.13430230561134948 1107 7 0.24431036432645925 1107 8 0.14063429862306265 1107 9 0.12841324696454848 1107 10-11 0.15677978545530635 1107 12-13 0.15263330829156274 1107 14-15 0.28507117736210574 1107 16-17 0.21726020900107557 1107 18-19 0.0869727675061327 1107 20-21 0.07583060773875872 1107 22-23 0.2129963510028361 1107 24-25 0.14863173587257705 1107 26-27 0.1449364620153304 1107 28-29 0.03107054764039674 1107 30-31 0.16854118741532886 1107 32-33 0.20371052512766852 1107 34-35 0.11088689018725972 1107 36-37 0.21225242221031237 1107 38-39 0.2161540702260396 1107 40-41 3.6327144027836766E-4 1107 42-43 -0.20962990908591195 1107 44-45 -0.16427767676767502 1107 46-47 -0.1662620224885316 1107 48-49 -0.19089668424523865 1107 50-51 -0.21623099519342048 1107 52-53 -0.18796891315105313 1107 54-55 -0.02493492676548925 1107 56-57 -0.18467202338570132 1107 58-59 -0.10022514474699307 1107 60-61 -0.20822736579646772 1107 62-63 -0.28216817151233187 1107 64-65 -0.35096810092907305 1107 66-67 -0.4801972901814153 1107 68-69 -0.2070254947436112 1107 70-71 -0.3615629659245201 1107 72-73 -0.15638135188107327 1107 74-75 -0.13511923410517213 1107 76-77 -0.4391266636346991 1107 78-79 -0.44601410358035665 1107 80-81 -0.6122778404348956 1107 82-83 -0.6109531471761507 1107 84-85 -0.6794373045577764 1107 86-87 -0.6449113990594704 1107 88-89 -0.797309259389916 1107 90-91 -0.6790772455276191 1107 92-93 -0.5992988035484501 1107 94-95 -0.7116555758091785 1107 96-97 -0.30211219176220183 1107 98-99 -0.4054551980435832 1107 100-101 -0.5095228648469146 1108 1 0.1999644712022004 1108 2 0.32181139050356933 1108 3 0.2415915165136795 1108 4 0.2657543808930072 1108 5 0.2979001975968174 1108 6 0.27893416363413337 1108 7 0.35977884113403036 1108 8 0.20728496656962392 1108 9 0.27119153898689774 1108 10-11 0.19252264031263877 1108 12-13 0.2064335756987461 1108 14-15 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-1.090149338322199 2316 60-61 -1.1614077067498485 2316 62-63 -1.3821922825004762 2316 64-65 -1.3661441665700877 2316 66-67 -1.4605944101654558 2316 68-69 -1.516753770373775 2316 70-71 -1.4465361851756242 2316 72-73 -1.2711473459651046 2316 74-75 -1.6241796815533824 2316 76-77 -1.371638991647604 2316 78-79 -1.410341388535283 2316 80-81 -1.306657208819388 2316 82-83 -1.0652143255291016 2316 84-85 -1.1431262487921074 2316 86-87 -1.0817198247874664 2316 88-89 -1.193407990653089 2316 90-91 -1.2125009873682195 2316 92-93 -1.2009455563249105 2316 94-95 -1.348599060022483 2316 96-97 -1.3299991823598383 2316 98-99 -1.4625589787573787 2316 100-101 -1.3557631211439443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3141.0 3 594.0 4 518.0 5 562.0 6 599.0 7 678.0 8 914.0 9 1112.0 10 1477.0 11 2321.0 12 3888.0 13 6375.0 14 6061.0 15 7372.0 16 10541.0 17 15111.0 18 12003.0 19 6442.0 20 5807.0 21 5825.0 22 6120.0 23 6874.0 24 7767.0 25 8490.0 26 10072.0 27 11564.0 28 13601.0 29 16061.0 30 19446.0 31 23574.0 32 29806.0 33 38179.0 34 51658.0 35 77774.0 36 111736.0 37 187302.0 38 211059.0 39 40228.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07778065989335 21.298972959852826 15.068707204490266 18.55453917576356 2 13.620452257543866 24.976288925680343 38.56585438904465 22.83740442773114 3 22.33055148771883 27.784480463289622 32.42798542235239 17.456982626639153 4 10.504775039575312 18.062047432129123 43.30350363718436 28.129673891111207 5 9.68183501354292 42.317461836428166 35.86273180027211 12.13797134975681 6 24.30275281803961 40.96216109468301 18.399862800837745 16.33522328643963 7 23.083104039350903 37.33460428207162 23.12800997077996 16.45428170779752 8 23.053005487771284 31.420219406896564 21.80014283382729 23.72663227150486 9 23.524764496632994 20.69841223640359 25.77719749433981 29.999625772623606 10-11 22.90691522259539 27.00732923993568 28.759496776111522 21.326258761357412 12-13 27.406162307749483 26.66821553368084 27.70829532631977 18.217326832249906 14-15 22.66853354455248 28.105191708401158 31.205705827444767 18.020568919601594 16-17 17.205720011725255 31.31178367320634 31.671441312396965 19.811055002671445 18-19 16.52087316016911 32.893665856876 33.156302073750204 17.429158909204688 20-21 19.154099686520702 28.809272458782747 31.462385343036587 20.574242511659964 22-23 17.781876288509743 28.900669219341136 32.31980097040443 20.99765352174469 24-25 20.421893065386822 29.25734884339935 32.03999026808514 18.280767823128684 26-27 19.84479875148617 26.752434409615056 35.98585336512843 17.416913473770347 28-29 16.884382717942614 31.424564234335904 34.02192564991217 17.669127397809305 30-31 17.92793851163303 29.25032722615148 33.00186199217568 19.819872270039806 32-33 18.337398019011502 29.821746380246665 33.25058423359364 18.59027136714819 34-35 20.342786453275966 28.241900877349636 32.69305362552983 18.722259043844574 36-37 19.61135632742364 27.31202896790374 35.14756333992089 17.929051364751718 38-39 18.83093769819948 29.5010988426844 34.016594426728275 17.651369032387848 40-41 17.9081395518484 30.213863061778724 34.035719570731544 17.842277815641328 42-43 17.04647028379599 31.018462297000994 31.35849003500868 20.576577384194337 44-45 18.566995633410844 28.901534639504888 31.55964431350411 20.971825413580163 46-47 20.41291768753584 30.435646145024243 30.94938226554762 18.202053901892302 48-49 19.55539915281851 29.58477680026067 31.67384815900945 19.185975887911372 50-51 17.043375811618493 30.525717651903967 33.93671786824868 18.494188668228865 52-53 18.446728981706904 32.2222280146595 30.538314365997465 18.79272863763613 54-55 17.06605647090711 29.463437772020995 32.12430479903675 21.34620095803514 56-57 19.64068617309826 29.629216603934044 30.397224374684406 20.33287284828329 58-59 21.39829570173999 28.867300763573557 30.355578828353565 19.378824706332892 60-61 18.92550633475043 29.69367066588963 33.19677958130807 18.184043418051864 62-63 18.57601501016313 29.504195549069685 32.642831083546156 19.276958357221037 64-65 17.863399009025542 31.823063512453913 31.42605854089855 18.887478937621996 66-67 18.400299473314863 29.39108482957028 30.24930736368594 21.95930833342892 68-69 17.23835679867431 30.093706672200803 31.310261326430954 21.35767520269393 70-71 19.968870175072677 29.979481430303345 29.85485784307335 20.196790551550624 72-73 19.116434163975654 27.474227287846293 32.94387090652589 20.465467641652165 74-75 17.339212964942227 32.092392522760285 30.656443003617245 19.911951508680243 76-77 18.49746869226888 28.74019483146161 30.8955074500515 21.86682902621801 78-79 18.3111253327552 28.97461367042799 31.921256100077755 20.79300489673905 80-81 19.59867189832966 29.075754014557837 30.77705204580502 20.548522041307486 82-83 18.40944711978246 28.34068175338393 32.5092921592471 20.740578967586508 84-85 17.992797126356795 29.10418624677531 31.561023998632194 21.341992628235705 86-87 18.052291322325896 29.577888383812322 31.496136867931078 20.873683425930704 88-89 18.2933803493147 28.81228518417923 30.32155448847443 22.572779978031637 90-91 18.47331173883027 27.02829116287025 31.712372482234592 22.786024616064893 92-93 19.65264210060333 28.879802320203517 31.48078915493347 19.986766424259677 94-95 18.71100180042829 27.915293837925997 31.440806683640293 21.93289767800542 96-97 16.803682158820475 27.686949756698866 32.92501569516547 22.58435238931519 98-99 18.543498333935528 28.580813381487836 30.168045451677873 22.707642832898763 100-101 18.01645844703444 26.643042899655807 31.959380882159 23.381117771150752 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4026.0 1 2369.0 2 944.0 3 1085.5 4 826.0 5 833.5 6 1593.0 7 3054.0 8 4276.0 9 4224.5 10 3243.5 11 2558.5 12 2537.5 13 2604.0 14 2569.0 15 2635.5 16 2818.0 17 2895.5 18 2983.0 19 3235.5 20 3594.0 21 4038.0 22 4491.5 23 5041.5 24 5684.0 25 6880.5 26 9289.0 27 11981.0 28 12909.0 29 14130.5 30 17229.0 31 20094.5 32 23654.5 33 28510.5 34 33457.0 35 36061.0 36 38375.5 37 41045.0 38 39067.5 39 35815.5 40 33144.0 41 30696.0 42 28219.5 43 26188.0 44 25397.5 45 24576.0 46 28104.0 47 45785.5 48 91778.5 49 89072.0 50 38370.0 51 16758.5 52 9504.0 53 6928.0 54 5524.5 55 4545.5 56 3682.0 57 2937.0 58 2343.5 59 1849.0 60 1422.0 61 1086.5 62 904.5 63 753.5 64 537.5 65 387.5 66 296.5 67 250.0 68 200.5 69 136.5 70 122.5 71 105.5 72 83.0 73 77.0 74 62.0 75 49.0 76 45.5 77 29.5 78 16.5 79 14.0 80 9.0 81 5.0 82 3.5 83 4.0 84 4.5 85 2.0 86 2.0 87 2.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28296389094048097 2 0.11333098568871687 3 0.12413430604767796 4 0.12558859917292273 5 0.13286006479914655 6 0.05837948117053975 7 0.0681440207257546 8 0.07375343706598439 9 0.07115648505661876 10-11 0.06544319063601432 12-13 0.06996188713231055 14-15 0.0657548248771382 16-17 0.06658584952013522 18-19 0.07167587545849188 20-21 0.0749480349902926 22-23 0.08310246429970072 24-25 0.09172434497079468 26-27 0.09177628401098199 28-29 0.0857513553492537 30-31 0.08247919581745297 32-33 0.07510385211085453 34-35 0.24219174439344032 36-37 0.3673648312448645 38-39 0.33744794409697226 40-41 0.3200483656342224 42-43 0.34627758092881544 44-45 0.35853519441302134 46-47 0.3653392086775593 48-49 0.3744285407103391 50-51 0.3687152462897347 52-53 0.3700137222944175 54-55 0.3544320102382236 56-57 0.3294493319081261 58-59 0.31521803489680233 60-61 0.2706543384160878 62-63 0.34456359260263414 64-65 0.37510374823277415 66-67 0.37915499336738456 68-69 0.391672302052527 70-71 0.3926072047758986 72-73 0.3749998701523995 74-75 0.37915499336738456 76-77 0.3613918416233235 78-79 0.3379153954586581 80-81 0.33786345641847076 82-83 0.35219863151016917 84-85 0.3600933656186407 86-87 0.3538606807961632 88-89 0.36985790517385553 90-91 0.39276302189646056 92-93 0.3887637158020375 94-95 0.3873094226767927 96-97 0.39193199725346356 98-99 0.3812844940150644 100-101 0.35978173137751684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962667.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.4299940012576 #Duplication Level Percentage of deduplicated Percentage of total 1 71.81166930850327 19.697936583525394 2 12.676329345464463 6.954232758081119 3 5.155241844942475 4.242247586454129 4 2.6676417803252983 2.9269359212730834 5 1.5379312640318836 2.1092722673370545 6 1.0295445415117626 1.6944240358617093 7 0.738852529033535 1.4186704307442712 8 0.5459764612310749 1.198090484511699 9 0.4128827344126903 1.019283383534664 >10 2.784151185942549 15.044480327912687 >50 0.366239289058705 7.029657972417855 >100 0.24200666500064646 12.576393169884884 >500 0.013676985777116594 2.6463344007893195 >1k 0.011777404419183733 7.221718817055173 >5k 0.004938911530625437 8.784988185655653 >10k+ 0.0011397488147597162 5.435333674961351 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 25282 2.6262456280312922 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15504 1.6105257581282 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11371 1.1811976519398713 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9366 0.9729221007887463 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8551 0.8882614652834261 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7034 0.7306784173551186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7023 0.7295357584709977 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6366 0.6612878596648686 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6303 0.6547435406012672 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6100 0.6336562902852181 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6028 0.626177068498245 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6011 0.6244111411318763 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5501 0.5714333201408172 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5462 0.5673820750062067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5377 0.5585524381743635 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5178 0.5378807001798129 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4426 0.45976438373809425 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4239 0.44033918270803923 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3961 0.4114610763638932 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3755 0.3900621918067203 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3470 0.3604569388999519 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3323 0.3451868610848819 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3309 0.34373256795963714 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3273 0.3399929570661506 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2911 0.302389091970536 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2687 0.2791204019666198 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2470 0.25657885852532597 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2441 0.2535663941944618 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2318 0.24078939030838287 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2260 0.23476446164665457 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2059 0.21388496749135474 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2038 0.2117035278034876 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1899 0.19726447463141458 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.18054010369109982 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.1752423215919939 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1660 0.172437613421879 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1430 0.14854565493571506 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1413 0.1467797275693464 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1396 0.14501380020297777 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 0.1326523086383973 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1222 0.12693901421779286 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1201 0.12475757452992571 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1168 0.12132959787756308 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1122 0.11655120618033028 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1075 0.11166893640272284 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1059 0.11000688711672883 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1012 0.10512461733912142 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 969 0.1006578598830125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0387808037462591E-4 0.0 12-13 0.0 0.0 0.0 1.0387808037462591E-4 0.0 14-15 0.0 0.0 0.0 1.0387808037462591E-4 0.0 16-17 0.0 0.0 0.0 1.5581712056193887E-4 0.0 18-19 0.0 0.0 0.0 2.0775616074925182E-4 0.0 20-21 0.0 0.0 0.0 6.232684822477555E-4 0.0 22-23 0.0 0.0 0.0 0.0015581712056193884 0.0 24-25 0.0 0.0 0.0 0.0028047081701148995 0.0 26-27 0.0 0.0 0.0 0.004778391697232792 0.0 28-29 0.0 0.0 0.0 0.01511426069450807 0.0 30-31 0.0 0.0 0.0 0.041447354069475736 0.0 32-33 0.0 0.0 0.0 0.09774927363252298 0.0 34-35 0.0 0.0 0.0 0.18578594675001842 0.0 36-37 0.0 0.0 0.0 0.3106993384005061 0.0 38-39 0.0 0.0 0.0 0.4850067572691284 0.0 40-41 0.0 0.0 0.0 0.680453365493987 0.0 42-43 0.0 0.0 0.0 0.876627120281468 0.0 44-45 0.0 0.0 0.0 1.1088985079991316 0.0 46-47 0.0 0.0 0.0 1.3963810954359088 0.0 48-49 0.0 0.0 0.0 1.6736316919557854 0.0 50-51 0.0 0.0 0.0 1.98048754138243 0.0 52-53 0.0 0.0 0.0 2.266411957613588 0.0 54-55 0.0 0.0 0.0 2.5786694672197132 0.0 56-57 0.0 0.0 0.0 2.9951166914415888 0.0 58-59 0.0 0.0 0.0 3.417017514883132 0.0 60-61 0.0 0.0 0.0 3.9176579232486413 0.0 62-63 0.0 0.0 0.0 4.391082274555999 0.0 64-65 0.0 0.0 0.0 4.844094583069742 0.0 66-67 0.0 0.0 0.0 5.383377637334613 0.0 68-69 0.0 0.0 0.0 6.009035315430985 0.0 70-71 0.0 0.0 0.0 6.690787156929655 0.0 72-73 0.0 0.0 0.0 7.334883194292523 0.0 74-75 0.0 0.0 0.0 8.04644804485871 0.0 76-77 0.0 0.0 0.0 8.721032298811531 0.0 78-79 0.0 0.0 0.0 9.623213426865156 0.0 80-81 0.0 0.0 0.0 10.56814038499294 0.0 82-83 0.0 0.0 0.0 11.406124859375048 0.0 84-85 0.0 0.0 0.0 12.354635611275757 0.0 86-87 0.0 0.0 0.0 13.312235695209246 0.0 88-89 0.0 0.0 0.0 14.317463878994502 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 260 0.0 40.10345 9 GTAAGCG 105 0.0 36.227234 94-95 CCTTTAG 335 0.0 31.189924 1 TAGGCAT 340 0.0 30.688082 5 TTAGGCA 380 0.0 29.953917 4 GTGACCC 150 3.4078766E-8 28.43699 9 CTGACCG 170 3.6998244E-9 27.898256 4 GGATATG 365 0.0 27.325172 1 TGACCGT 175 5.0404196E-9 27.101166 5 CATGGGG 1830 0.0 26.693905 4 CGGAAAG 45 8.9171913E-4 26.405363 76-77 GTACATG 5635 0.0 25.622183 1 CCGTGCA 185 9.1786205E-9 25.61891 8 ACCGTGC 185 9.1786205E-9 25.61891 7 GGCATTC 410 0.0 25.431454 7 CGTGCAA 195 1.6063495E-8 24.305119 9 ATGGGGA 1015 0.0 24.297594 5 TACATGG 5765 0.0 24.186554 2 GTGTACG 100 0.001674887 23.697493 9 ACATGGG 5585 0.0 23.437532 3 >>END_MODULE