##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939338_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962667 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14278769294055 33.0 31.0 34.0 30.0 34.0 2 32.324803904153775 34.0 31.0 34.0 30.0 34.0 3 32.41010650619581 34.0 31.0 34.0 30.0 34.0 4 35.94321712492482 37.0 35.0 37.0 35.0 37.0 5 35.90604019873955 37.0 35.0 37.0 35.0 37.0 6 35.98824619520561 37.0 35.0 37.0 35.0 37.0 7 35.94562813517032 37.0 35.0 37.0 35.0 37.0 8 35.95352390805959 37.0 35.0 37.0 35.0 37.0 9 37.581259147763454 39.0 37.0 39.0 35.0 39.0 10-11 37.49934764565525 39.0 37.0 39.0 34.5 39.0 12-13 37.46221434826373 39.0 37.0 39.0 34.0 39.0 14-15 38.55878772202641 40.0 38.0 41.0 34.0 41.0 16-17 38.56824218551171 40.0 38.0 41.0 34.0 41.0 18-19 38.81249019650616 40.0 38.0 41.0 34.0 41.0 20-21 38.832705390337466 40.0 38.0 41.0 34.5 41.0 22-23 38.776849107739224 40.0 38.0 41.0 34.0 41.0 24-25 38.66762650012933 40.0 38.0 41.0 34.0 41.0 26-27 38.418700339785204 40.0 38.0 41.0 34.0 41.0 28-29 38.28949989975766 40.0 38.0 41.0 34.0 41.0 30-31 38.11103424133164 40.0 37.0 41.0 34.0 41.0 32-33 37.82890812710937 40.0 36.5 41.0 33.0 41.0 34-35 37.64003076868741 40.0 36.0 41.0 33.0 41.0 36-37 37.45984073412717 40.0 36.0 41.0 33.0 41.0 38-39 37.29366281382866 40.0 36.0 41.0 33.0 41.0 40-41 37.18739709577663 40.0 36.0 41.0 32.5 41.0 42-43 36.93764198835112 40.0 36.0 41.0 31.0 41.0 44-45 36.60894317557369 40.0 35.5 41.0 30.5 41.0 46-47 36.15270337510271 40.0 35.0 41.0 30.0 41.0 48-49 35.68481520608891 39.5 35.0 41.0 27.5 41.0 50-51 35.404065476431626 39.0 35.0 41.0 27.0 41.0 52-53 35.24508319076067 39.0 35.0 41.0 26.0 41.0 54-55 35.04561909777732 39.0 35.0 40.5 25.0 41.0 56-57 34.78962559223491 39.0 35.0 40.0 23.5 41.0 58-59 34.56461216599301 38.5 35.0 40.0 23.0 41.0 60-61 34.131722080428645 38.0 34.0 40.0 22.0 41.0 62-63 33.843326404665376 38.0 34.0 40.0 22.0 41.0 64-65 33.428707434658094 37.5 33.0 40.0 19.5 41.0 66-67 32.96562102990961 37.0 33.0 39.0 17.5 40.5 68-69 32.57317795250071 36.0 32.5 39.0 13.5 40.0 70-71 32.109623057609745 36.0 32.0 38.5 10.0 40.0 72-73 31.5660758081455 35.0 31.5 37.5 7.5 40.0 74-75 31.09341132499608 35.0 31.0 37.0 2.0 39.0 76-77 29.087822164881523 33.0 28.5 35.0 2.0 37.5 78-79 30.034029420349924 34.0 30.0 36.0 2.0 38.0 80-81 30.018623262249562 34.0 30.5 36.0 2.0 37.5 82-83 29.674099662707874 34.0 30.0 35.0 2.0 37.0 84-85 29.284860185297717 34.0 30.0 35.0 2.0 36.5 86-87 28.84748516361317 34.0 29.0 35.0 2.0 36.0 88-89 28.29393081927603 34.0 29.0 35.0 2.0 36.0 90-91 27.826198467382802 34.0 27.5 35.0 2.0 35.0 92-93 27.536307986042942 34.0 27.0 35.0 2.0 35.0 94-95 27.15964710538535 33.0 26.5 35.0 2.0 35.0 96-97 26.59756592882066 33.0 25.0 35.0 2.0 35.0 98-99 25.97290028639187 33.0 24.5 35.0 2.0 35.0 100-101 22.85302238468754 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1491966318211695 1101 2 0.0997032559441422 1101 3 0.20160345648214673 1101 4 0.14194611141979863 1101 5 0.24901924966687972 1101 6 0.21455361158546538 1101 7 0.14127162365215185 1101 8 0.21935962252909746 1101 9 0.27584133609344264 1101 10-11 0.2309663874223915 1101 12-13 0.2774790515562202 1101 14-15 0.40019922318739276 1101 16-17 0.2960913342695122 1101 18-19 0.3663031706325768 1101 20-21 0.3572185085602939 1101 22-23 0.40229682372791586 1101 24-25 0.34327017543450467 1101 26-27 0.4034418257729584 1101 28-29 0.27682399925641477 1101 30-31 0.20618675503978068 1101 32-33 0.3109705909734686 1101 34-35 0.39362188257528175 1101 36-37 0.28699410814275694 1101 38-39 0.3190487405729243 1101 40-41 0.35522492626905233 1101 42-43 0.4486219079753795 1101 44-45 0.4101743603112453 1101 46-47 0.23696434780702447 1101 48-49 0.4086768295442553 1101 50-51 0.545874738663251 1101 52-53 0.5409456722193795 1101 54-55 0.4861534960479261 1101 56-57 0.2906138063592181 1101 58-59 0.3371071619445374 1101 60-61 0.6377524923055873 1101 62-63 0.5461913392319317 1101 64-65 0.4422283451735254 1101 66-67 0.36830946424576894 1101 68-69 0.3577782567947523 1101 70-71 0.4144026571460131 1101 72-73 0.1260488218452842 1101 74-75 -0.21246630057986948 1101 76-77 -0.0015301366265916272 1101 78-79 0.4982734495245005 1101 80-81 0.45969235039974166 1101 82-83 0.3880900074061877 1101 84-85 0.3749096787339887 1101 86-87 0.42339075777610446 1101 88-89 0.36192842520446433 1101 90-91 0.6095150200534967 1101 92-93 0.4413225662964173 1101 94-95 0.3790090949196703 1101 96-97 0.36164616870296484 1101 98-99 0.3550431326346022 1101 100-101 -0.1730781028730597 1102 1 0.1337424598966166 1102 2 0.1557061686804957 1102 3 0.22125565353636034 1102 4 0.17058948106972593 1102 5 0.1072894761496741 1102 6 0.17057889624256717 1102 7 0.1806140354036927 1102 8 0.26472929293075254 1102 9 0.3626721707415541 1102 10-11 0.24280010994915813 1102 12-13 0.15644038030209373 1102 14-15 0.3090153684030881 1102 16-17 0.40797433351061585 1102 18-19 0.30925477271068047 1102 20-21 0.3054055787598742 1102 22-23 0.21551760534141806 1102 24-25 0.3899434123297496 1102 26-27 0.5081182883190039 1102 28-29 0.316008491567338 1102 30-31 0.33722114199797915 1102 32-33 0.2640348861190631 1102 34-35 0.5994676800903846 1102 36-37 0.6607587175187817 1102 38-39 0.6760658470501042 1102 40-41 0.8506006648714433 1102 42-43 0.8774265601651265 1102 44-45 0.7470655114123659 1102 46-47 0.6775983358724105 1102 48-49 0.7456279315441279 1102 50-51 0.6075064559055718 1102 52-53 0.7280568138745807 1102 54-55 0.8024083780250848 1102 56-57 0.7699095441427559 1102 58-59 0.4479285916126301 1102 60-61 0.46840874993652903 1102 62-63 0.45796116287393573 1102 64-65 0.28843674327089275 1102 66-67 0.37967673953590975 1102 68-69 0.5411936448744754 1102 70-71 0.3695147872599307 1102 72-73 0.5187330575371227 1102 74-75 0.4272595044802756 1102 76-77 0.603982714067886 1102 78-79 0.6399900630883195 1102 80-81 0.7289983675690976 1102 82-83 0.6305241308728711 1102 84-85 0.5275819534904187 1102 86-87 0.6128992042728569 1102 88-89 0.8563478738609049 1102 90-91 0.9107741351489871 1102 92-93 0.8732446652212467 1102 94-95 0.9804430911734734 1102 96-97 0.8547752108192022 1102 98-99 0.5483306790782727 1102 100-101 0.5646776571654257 1103 1 0.22596028847465988 1103 2 0.271586275028433 1103 3 0.2535087384672323 1103 4 0.08023508443162086 1103 5 0.17945887203661925 1103 6 0.06419512574240827 1103 7 0.19509973783621604 1103 8 0.22602311011319642 1103 9 0.177726550702495 1103 10-11 0.3537060107710275 1103 12-13 0.39934466026895876 1103 14-15 0.5267006055289372 1103 16-17 0.35635988750259884 1103 18-19 0.3215207583242119 1103 20-21 0.3657328095373771 1103 22-23 0.29864637923903103 1103 24-25 0.4000000182097594 1103 26-27 0.26976163681798937 1103 28-29 0.32485634758690196 1103 30-31 0.45231976468470947 1103 32-33 0.295196374824755 1103 34-35 0.519576481360609 1103 36-37 0.6908685800631176 1103 38-39 0.5776368083852716 1103 40-41 0.7023737506844085 1103 42-43 0.6626205459109684 1103 44-45 0.6545789186122235 1103 46-47 0.6540525225987608 1103 48-49 0.6600175679957303 1103 50-51 0.6180103130167325 1103 52-53 0.4419386926881046 1103 54-55 0.626893625625847 1103 56-57 0.7604442195513812 1103 58-59 0.5722499619518047 1103 60-61 0.5167070589271745 1103 62-63 0.511290186791193 1103 64-65 0.6003396904428726 1103 66-67 0.5429599632731623 1103 68-69 0.3051807552180392 1103 70-71 0.4761535392015759 1103 72-73 0.6426292290941511 1103 74-75 0.6655072720143878 1103 76-77 0.6578279031194008 1103 78-79 0.7262199626256312 1103 80-81 0.7108974417797214 1103 82-83 0.6476940298313068 1103 84-85 0.5974317073988722 1103 86-87 0.6160837157353072 1103 88-89 0.8533721715248639 1103 90-91 0.9026112833262836 1103 92-93 0.8104916650544745 1103 94-95 0.7506820708066577 1103 96-97 0.6222493646598437 1103 98-99 0.49821442132373406 1103 100-101 0.6014652769205604 1104 1 0.12399738539622973 1104 2 0.1071926456242096 1104 3 0.13788112725526247 1104 4 0.02963898322352776 1104 5 -0.01614813418857608 1104 6 0.07489356328585473 1104 7 0.1150819402126757 1104 8 -3.1790241797580165E-4 1104 9 0.12947300872380652 1104 10-11 0.12306986490996508 1104 12-13 0.19241947782345648 1104 14-15 0.16798248466135846 1104 16-17 0.22122904716078295 1104 18-19 0.032779476709926314 1104 20-21 0.1090093732188393 1104 22-23 0.07078912571427054 1104 24-25 0.15136562827495226 1104 26-27 0.13008472195127752 1104 28-29 0.21372051755420074 1104 30-31 0.11718018443847456 1104 32-33 0.06928639848871398 1104 34-35 0.12089978234305221 1104 36-37 -0.08614004198501846 1104 38-39 0.045613666177764856 1104 40-41 -0.04579318678687372 1104 42-43 -0.0656094777794678 1104 44-45 -0.03254044861870398 1104 46-47 -0.19832850545235914 1104 48-49 -0.3825292136668921 1104 50-51 -0.37467465183164705 1104 52-53 -0.34124149368373224 1104 54-55 -0.3812768607763246 1104 56-57 -0.459900026744414 1104 58-59 -0.4146752734628123 1104 60-61 -0.3098554969386882 1104 62-63 -0.37257105001121715 1104 64-65 -0.050369367408670485 1104 66-67 -0.10270444823331815 1104 68-69 -0.1317343498528274 1104 70-71 -0.34732488978735887 1104 72-73 -0.2731366315149657 1104 74-75 -0.21256112965573237 1104 76-77 -0.0928371903644738 1104 78-79 -0.18931813077746185 1104 80-81 -0.14716386202862708 1104 82-83 -0.19614771433580103 1104 84-85 -0.029829246283608057 1104 86-87 -0.22418857201412834 1104 88-89 -0.13834412058393397 1104 90-91 0.006636009310415858 1104 92-93 -0.10910121430703512 1104 94-95 0.12033434179136648 1104 96-97 0.1756329656858533 1104 98-99 0.22743761597067547 1104 100-101 0.3180142859345807 1105 1 0.10301527540580224 1105 2 0.1343085838907001 1105 3 0.03835785836005812 1105 4 -0.019129407605561255 1105 5 -0.023506403232310902 1105 6 0.010986279800697218 1105 7 0.18565390129406012 1105 8 0.07911058856210929 1105 9 -0.1362745524045934 1105 10-11 -0.05106614876037696 1105 12-13 0.11571950068484682 1105 14-15 0.17988913784368066 1105 16-17 0.12609271188100735 1105 18-19 0.11643990880318711 1105 20-21 0.2072061646157124 1105 22-23 0.16878894747289053 1105 24-25 0.09945476446298329 1105 26-27 0.10296361726805259 1105 28-29 0.1657871488309226 1105 30-31 0.10810149861990936 1105 32-33 0.147381953949548 1105 34-35 0.17169276273262568 1105 36-37 0.2960372410222547 1105 38-39 0.3155453790644813 1105 40-41 0.2543155662855341 1105 42-43 0.04847146188841123 1105 44-45 -0.012802153458729038 1105 46-47 0.24232680284710284 1105 48-49 0.12937291890077773 1105 50-51 0.29507442822328045 1105 52-53 0.36197757142732456 1105 54-55 0.2970626787151147 1105 56-57 0.3986990218599331 1105 58-59 0.4478412106356018 1105 60-61 0.27562480545167745 1105 62-63 0.04006096808157622 1105 64-65 0.13354925636123482 1105 66-67 -0.059670779987015976 1105 68-69 0.1642845385667826 1105 70-71 0.057348263315525116 1105 72-73 0.13739911298506158 1105 74-75 0.12443131833459731 1105 76-77 0.15955970410242415 1105 78-79 0.15762354425234193 1105 80-81 0.3588989280172399 1105 82-83 0.30655109260941416 1105 84-85 0.09053793095419493 1105 86-87 0.20744811006137098 1105 88-89 0.4051629205962861 1105 90-91 0.42272113119264887 1105 92-93 0.34977346263046627 1105 94-95 0.15836756443421862 1105 96-97 0.2722385007264343 1105 98-99 0.061147451924025376 1105 100-101 -0.0478784515042463 1106 1 0.10194093577434415 1106 2 0.10066724861192 1106 3 0.13330170137371766 1106 4 -0.015628584641738996 1106 5 0.01909964478353743 1106 6 -0.010179686004818223 1106 7 0.06091126955531223 1106 8 0.08044306471828122 1106 9 -0.032637654615591316 1106 10-11 0.01244957236859534 1106 12-13 0.15570177531014906 1106 14-15 0.09979183266607805 1106 16-17 0.05733067319248164 1106 18-19 -0.06005233542346389 1106 20-21 -0.08125709948376425 1106 22-23 0.10657435735066656 1106 24-25 0.04781578191424529 1106 26-27 -0.0831485147798432 1106 28-29 0.013712161399702438 1106 30-31 -0.042564800919258516 1106 32-33 0.002264975081104126 1106 34-35 0.1183986333718039 1106 36-37 0.10872231569131685 1106 38-39 0.19743992906492025 1106 40-41 0.36275503265587616 1106 42-43 0.3132838888265539 1106 44-45 0.35197179884501395 1106 46-47 0.4478434262246864 1106 48-49 0.17415653288587407 1106 50-51 0.3041559170072432 1106 52-53 0.3812056694651105 1106 54-55 0.37644150389892417 1106 56-57 0.4233252463182495 1106 58-59 0.46318496858712876 1106 60-61 0.3172530077274658 1106 62-63 0.429047511793307 1106 64-65 0.4045631060004027 1106 66-67 0.3348484899045445 1106 68-69 0.43147489458620925 1106 70-71 0.4010250862203435 1106 72-73 0.47983011985271773 1106 74-75 0.46337010678925594 1106 76-77 0.56865815614373 1106 78-79 0.6809019272851522 1106 80-81 0.6460143506912139 1106 82-83 0.6983064578541587 1106 84-85 0.6406287089653837 1106 86-87 0.6502886935482586 1106 88-89 0.9830980250739678 1106 90-91 1.0695768439383215 1106 92-93 1.048000187627796 1106 94-95 1.0943865829499835 1106 96-97 1.1493231068162402 1106 98-99 1.2233899453753487 1106 100-101 1.3479198234062366 1107 1 -0.09366686236360522 1107 2 -0.0491183097069694 1107 3 0.07011312309006001 1107 4 -0.028653931197986537 1107 5 -0.009115617205161186 1107 6 0.01582714673067187 1107 7 -0.03555666613826247 1107 8 0.07539437969980156 1107 9 0.06378760611656276 1107 10-11 0.017146781663200272 1107 12-13 0.15372568751352844 1107 14-15 -5.743422111805785E-4 1107 16-17 0.15352892683822716 1107 18-19 0.13405531927919867 1107 20-21 0.11123957542549334 1107 22-23 0.13109052997562998 1107 24-25 0.09711958733538495 1107 26-27 -0.006562962150944429 1107 28-29 4.8055252995737874E-4 1107 30-31 0.24015207679089912 1107 32-33 0.28143212579246324 1107 34-35 0.25051557224941234 1107 36-37 0.2627249899888895 1107 38-39 0.24516476091371686 1107 40-41 0.3257107953167022 1107 42-43 0.1565861721740731 1107 44-45 0.08770176591416856 1107 46-47 -0.021895284469898968 1107 48-49 0.10368476118743075 1107 50-51 -0.13437020836705216 1107 52-53 -0.16444705592387976 1107 54-55 -0.15938886787112239 1107 56-57 -0.15137668093041867 1107 58-59 -0.06219122305189728 1107 60-61 -0.3552866029346333 1107 62-63 -0.29299623952547194 1107 64-65 -0.22807245219994599 1107 66-67 -0.06975400468402881 1107 68-69 -0.17570564183805715 1107 70-71 -0.04542613511333826 1107 72-73 -0.06598441476497285 1107 74-75 -0.14032649320173363 1107 76-77 0.1171866997658384 1107 78-79 0.3521470641176734 1107 80-81 0.17840642452838296 1107 82-83 0.10924736962841664 1107 84-85 -0.04985809380279704 1107 86-87 -0.13421756900233106 1107 88-89 0.14346163945353752 1107 90-91 0.2683188809090602 1107 92-93 0.224580880052752 1107 94-95 0.31780719448218164 1107 96-97 0.0032976867642240393 1107 98-99 0.1388709988778949 1107 100-101 -0.05016525624183643 1108 1 -0.0021518978464811767 1108 2 0.05813825262518435 1108 3 0.05891516178410683 1108 4 0.05963843398422597 1108 5 0.13230392637421318 1108 6 0.17731937020993627 1108 7 0.16065049443174928 1108 8 0.09363863444513498 1108 9 0.2258352137365307 1108 10-11 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-0.7188868218289244 2316 64-65 -1.0335147918341647 2316 66-67 -0.8565842319185464 2316 68-69 -0.6625249683193104 2316 70-71 -0.5799140860858145 2316 72-73 -0.7673873786613079 2316 74-75 -0.8588681202222972 2316 76-77 -0.9019801501197016 2316 78-79 -1.0154577447773399 2316 80-81 -1.189473212679772 2316 82-83 -1.1974693419653661 2316 84-85 -1.3007925100674598 2316 86-87 -1.253293979587518 2316 88-89 -1.4505689048266177 2316 90-91 -1.8169037623573168 2316 92-93 -1.6023539574613572 2316 94-95 -1.5475597267201557 2316 96-97 -1.4976196393364312 2316 98-99 -1.2473971929088457 2316 100-101 -0.7522347365345716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 4.0 5 38.0 6 85.0 7 188.0 8 392.0 9 713.0 10 1155.0 11 2107.0 12 3122.0 13 4845.0 14 5432.0 15 7443.0 16 10093.0 17 14878.0 18 13397.0 19 7441.0 20 5984.0 21 5843.0 22 6291.0 23 6668.0 24 7792.0 25 8962.0 26 10430.0 27 12283.0 28 14980.0 29 17885.0 30 21777.0 31 26632.0 32 32437.0 33 40927.0 34 54931.0 35 79830.0 36 116943.0 37 204722.0 38 197051.0 39 18959.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.995291568574345 21.88472676514094 15.597929123523407 18.52205254276131 2 13.83001598683657 25.623709964089347 38.12377488788958 22.4224991611845 3 22.28641887589374 27.726513945112902 32.69749560336025 17.28957157563311 4 10.687704055504135 18.732645868197416 42.65057387445503 27.929076201843422 5 9.537150437274779 42.60507527525094 35.83804160732632 12.019732680147964 6 24.120490262988138 41.736550645238694 17.855291601353322 16.287667490419842 7 22.934825853592155 37.395070154061585 22.846944997595223 16.82315899475104 8 23.148295308762012 31.98322992270432 21.316405361355486 23.552069407178184 9 23.16128006880884 20.858199148823008 25.337006462255378 30.643514320112768 10-11 23.036003103877043 27.30591159767604 28.58626087733349 21.071824421113426 12-13 27.49221693482793 26.713131332018236 27.598120637769863 18.196531095383968 14-15 22.35995003363121 28.483583035327054 31.09549445669365 18.060972474348088 16-17 17.141018496059633 31.538921399926974 31.538194251475982 19.78186585253741 18-19 16.5109534241851 32.86338889771853 33.11217690021576 17.513480777880616 20-21 19.157507216929634 28.706343938246558 31.578728677725525 20.557420167098282 22-23 17.820804078668946 28.927915883685635 32.350179241627686 20.90110079601773 24-25 20.35407882476495 29.441644930178345 32.18870076568533 18.01557547937137 26-27 19.79934618185369 26.813763630300848 36.12064783468186 17.2662423531636 28-29 16.71725529180911 31.588649034401307 34.06764748350156 17.62644819028802 30-31 18.037545693370603 29.180079923795038 33.0509927108751 19.731381671959255 32-33 18.36798186704229 29.81248967711576 33.34803208170634 18.471496374135604 34-35 20.335547915641918 28.161103721090402 33.00577737448637 18.49757098878131 36-37 19.681784508671 27.293989055140937 35.22470001381875 17.799526422369322 38-39 18.63826520382754 29.53096967285579 34.25108793552482 17.57967718779185 40-41 17.924075463229443 30.17708383117814 33.970661269509456 17.92817943608296 42-43 17.116753990065543 30.952011173793736 31.548930277542453 20.382304558598268 44-45 18.757216321624995 28.855625783310124 31.50764832705993 20.879509568004952 46-47 20.46345301844359 30.554658162496818 30.964561919255402 18.01732689980419 48-49 19.72395438921247 29.620574923623643 31.624954423492234 19.030516263671654 50-51 16.97430160169612 30.691454054205657 33.81974244468752 18.5145018994107 52-53 18.519955498630367 32.25892234801858 30.361277575734913 18.859844577616144 54-55 17.10020183511017 29.602396259558084 31.795470292427186 21.501931612904563 56-57 19.716225712642956 29.615396401557547 30.345192234278436 20.32318565152106 58-59 21.41931509673961 28.93078200385078 30.213527808061297 19.43637509134831 60-61 18.778038114955685 30.104228941126 32.87805780039381 18.239675143524504 62-63 18.547068077974057 29.72762265965895 32.28389030944156 19.441418952925428 64-65 17.79348414353042 32.19571253611061 30.905910351139077 19.104892969219886 66-67 18.46438072562994 29.65620510519214 29.798258380104436 22.08115578907348 68-69 17.249406595233026 30.35728939973927 30.883848483117184 21.50945552191053 70-71 19.90584490794844 30.307572608181232 29.576426739464424 20.210155744405906 72-73 19.235623533371353 27.77253193471886 32.381446543820445 20.61039798808934 74-75 17.331434442024086 32.43967020787043 29.952984780822444 20.27591056928304 76-77 18.562138254375306 28.973904858883415 30.38251980588205 22.081437080859224 78-79 18.375201393628327 29.27943930767337 31.359597867175253 20.985761431523052 80-81 19.512889339494066 29.298368264468305 30.303659874347638 20.88508252168999 82-83 18.327521354736373 28.791420086073376 31.974452224912664 20.906606334277587 84-85 17.74694676352259 29.59953961234778 30.96169288030025 21.691820743829382 86-87 17.8976670091215 30.01271727511324 30.574804197636645 21.514811518128614 88-89 18.109535202531184 29.2572727225341 29.48792527378588 23.145266801148832 90-91 18.421044401121755 27.569301821290438 30.766620448077063 23.243033329510745 92-93 19.40364632837731 29.55139212209414 30.58155104516931 20.463410504359246 94-95 18.507282372824662 28.65279478781344 30.500630020557473 22.339292818804427 96-97 16.46654554482495 28.63607041687312 31.853693956477162 23.043690081824764 98-99 18.117739571419815 29.328521700650377 29.10201554639351 23.451723181536295 100-101 17.546040183262186 27.7923409453657 30.80070990464487 23.860908966727244 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3228.0 1 1936.0 2 799.5 3 957.5 4 756.0 5 733.5 6 1460.0 7 2830.0 8 4015.0 9 4095.5 10 3156.5 11 2388.0 12 2260.5 13 2301.5 14 2398.0 15 2385.0 16 2406.5 17 2551.0 18 2759.5 19 3086.0 20 3411.0 21 3837.5 22 4390.5 23 4962.5 24 5569.5 25 6798.0 26 9452.0 27 12154.0 28 12930.0 29 14119.0 30 17201.5 31 20111.0 32 23857.5 33 28822.0 34 33984.5 35 36493.5 36 38291.5 37 40969.5 38 39216.5 39 35927.0 40 33356.0 41 30827.0 42 28412.5 43 26599.5 44 25648.5 45 24772.5 46 27169.5 47 46145.0 48 91683.0 49 88083.5 50 41390.0 51 20038.0 52 9878.0 53 6288.5 54 5064.0 55 4270.0 56 3547.5 57 2811.0 58 2220.5 59 1773.5 60 1341.0 61 982.5 62 779.0 63 606.5 64 404.5 65 293.0 66 218.0 67 187.0 68 133.0 69 69.5 70 61.0 71 62.5 72 51.5 73 30.0 74 22.5 75 21.0 76 15.5 77 9.0 78 4.5 79 4.0 80 3.0 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2791204019666198 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002025622567305205 16-17 2.596952009365648E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0014542931252447626 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0033760376121753422 36-37 0.021398884557172936 38-39 0.006648197143976058 40-41 0.01921744486930579 42-43 0.00597298962154099 44-45 5.713294420604425E-4 46-47 2.0775616074925182E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.1939040187312955E-5 58-59 5.713294420604425E-4 60-61 0.0027527691299275864 62-63 0.0014023540850574498 64-65 0.0 66-67 0.0 68-69 2.0775616074925182E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23060933843166953 78-79 0.0 80-81 0.001142658884120885 82-83 0.0 84-85 0.0 86-87 0.10211215300825727 88-89 0.35650957184571613 90-91 0.35910652385508174 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.596952009365648E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962667.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.337141051371493 #Duplication Level Percentage of deduplicated Percentage of total 1 70.58991222405838 18.591364750489586 2 13.035549591818418 6.866382165637395 3 5.439792067156719 4.298057128885147 4 2.8149096397249656 2.965466889132069 5 1.693910180546938 2.230637567670943 6 1.0616269441602524 1.6776131143371034 7 0.7683448915705474 1.4165205449775968 8 0.5559012722903681 1.1712680175158638 9 0.42447575923001135 1.006153014935593 >10 2.9389209807079553 15.195664692165744 >50 0.38039705872451846 6.907820295514178 >100 0.2611032757296903 12.932071242680806 >500 0.015010475760557081 2.6859757479066255 >1k 0.013825438200513102 7.703271913151524 >5k 0.00513516276019058 9.006518415360306 >10k+ 0.0011850375600439803 5.345214499639573 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26780 2.781854992432482 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14339 1.489507794491761 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10260 1.0657891046436618 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9092 0.9444595067660988 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8045 0.8356991566138654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7354 0.763919403074999 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6887 0.7154083395400486 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6873 0.7139540464148039 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6745 0.7006576521268517 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6365 0.6611839815844939 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6310 0.6554706871638896 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6174 0.6413432682329403 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6017 0.6250344096141242 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5737 0.5959485471092288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5496 0.570913929738944 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5477 0.568940246211826 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4138 0.429847496590202 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4111 0.4270427884200871 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4027 0.4183170296686185 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3628 0.3768696755991428 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3611 0.37510374823277415 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3553 0.3690788195710459 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3529 0.3665857456420548 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3146 0.32680044085857307 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3090 0.32098326835759405 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2524 0.2621882748655558 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2444 0.2538780284355857 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2441 0.2535663941944618 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2367 0.24587941624673954 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2281 0.23694590133452167 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2228 0.23144036307466653 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2178 0.22624645905593524 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1940 0.20152347592677428 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.18687666659395202 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1611 0.16734758748352233 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1592 0.16537390395640444 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1535 0.15945285337505077 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.14937667957871206 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1421 0.14761075221234343 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1314 0.13649579761225844 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1249 0.12974372238790777 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1172 0.12174511019906156 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1144 0.11883652394857204 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1136 0.11800549930557502 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1093 0.11353874184946611 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1084 0.11260383912609448 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1081 0.11229220488497062 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1054 0.1094874967148557 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1035 0.10751381318773781 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.10657891046436617 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1025 0.10647503238399156 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 983 0.10211215300825727 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0387808037462591E-4 0.0 10-11 0.0 0.0 0.0 1.0387808037462591E-4 0.0 12-13 0.0 0.0 0.0 1.0387808037462591E-4 0.0 14-15 0.0 0.0 0.0 1.0387808037462591E-4 0.0 16-17 0.0 0.0 0.0 1.5581712056193887E-4 0.0 18-19 0.0 0.0 0.0 2.0775616074925182E-4 0.0 20-21 0.0 0.0 0.0 6.232684822477555E-4 0.0 22-23 0.0 0.0 0.0 0.0015581712056193884 0.0 24-25 0.0 0.0 0.0 0.0028047081701148995 0.0 26-27 0.0 0.0 0.0 0.004830330737420105 0.0 28-29 0.0 0.0 0.0 0.015373955895444635 0.0 30-31 0.0 0.0 0.0 0.0421225615919108 0.0 32-33 0.0 0.0 0.0 0.09894387155683118 0.0 34-35 0.0 0.0 0.0 0.1884348377995714 0.0 36-37 0.0 0.0 0.0 0.31360792465099563 0.0 38-39 0.0 0.0 0.0 0.4886424900822403 0.0 40-41 0.0 0.0 0.0 0.6850759400706579 0.0 42-43 0.0 0.0 0.0 0.8822365366216979 0.0 44-45 0.0 0.0 0.0 1.1158064003440442 0.0 46-47 0.0 0.0 0.0 1.40505491514719 0.0 48-49 0.0 0.0 0.0 1.6837598047923112 0.0 50-51 0.0 0.0 0.0 1.991550556942328 0.0 52-53 0.0 0.0 0.0 2.27835793685667 0.0 54-55 0.0 0.0 0.0 2.5932123984721613 0.0 56-57 0.0 0.0 0.0 3.012152696623027 0.0 58-59 0.0 0.0 0.0 3.435455874149628 0.0 60-61 0.0 0.0 0.0 3.9392645639665638 0.0 62-63 0.0 0.0 0.0 4.415337806323475 0.0 64-65 0.0 0.0 0.0 4.8716222743690185 0.0 66-67 0.0 0.0 0.0 5.411165023834825 0.0 68-69 0.0 0.0 0.0 6.036459128649886 0.0 70-71 0.0 0.0 0.0 6.719924958474738 0.0 72-73 0.0 0.0 0.0 7.365423349922663 0.0 74-75 0.0 0.0 0.0 8.07813085937297 0.0 76-77 0.0 0.0 0.0 8.75681829750059 0.0 78-79 0.0 0.0 0.0 9.661544438523393 0.0 80-81 0.0 0.0 0.0 10.607665994575488 0.0 82-83 0.0 0.0 0.0 11.445702407997782 0.0 84-85 0.0 0.0 0.0 12.39577133110411 0.0 86-87 0.0 0.0 0.0 13.353059780796475 0.0 88-89 0.0 0.0 0.0 14.355898768733113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 120 0.0 37.56732 94-95 ACTCCCG 140 4.6020432E-10 33.895332 6 GGTACTC 160 1.9244908E-9 29.658413 3 GTGCAGG 120 5.3066524E-6 27.757599 1 GACATAT 260 0.0 27.452568 1 CGCACCA 35 0.008213276 27.263535 84-85 CTCCCGC 180 6.7666406E-9 26.363035 7 CATGGGG 1880 0.0 25.493616 4 AGGGGTC 210 1.2787496E-9 24.856577 5 TACACTG 230 1.364242E-10 24.758327 5 TACTCCC 195 1.585613E-8 24.33511 5 ATGGGGA 1025 0.0 24.073952 5 CTTATGC 100 0.001662911 23.726732 3 GTACATG 5820 0.0 23.710466 1 AGTACTT 6635 0.0 23.31549 12-13 CATTCCG 185 2.5649024E-7 23.085468 9 GAGTACT 6475 0.0 22.975538 12-13 CTCCGAC 125 3.8744474E-10 22.777662 94-95 GTATAGG 420 0.0 22.659264 1 CGCTGTA 105 3.4851837E-8 22.596888 10-11 >>END_MODULE