FastQCFastQC Report
Fri 27 May 2016
SRR939337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163080.6316821849975094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120520.46682816369818386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87180.3376873490807142No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59830.23174849845720497No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45220.1751573976305333No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39320.1523040441139445No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32020.12402786094935153No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.11081939730671292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27960.10830165497013958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.10043855198053359No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25840.1000899415031619No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG172100.027.960551
TACATGG175700.026.8250562
ACATGGG174250.025.8509673
GTATCAA252900.023.793541
CATGGGA112350.022.53694
CATGGGG56300.022.4026414
GAGTACT126000.022.20522112-13
AGTACTT128750.022.00694312-13
TATAACG2005.483889E-721.3372522
ATGGGAT43650.020.7479465
GGTATCA190450.020.7315121
GTACTTT136750.020.4110514-15
ATCAACG297700.020.1323383
TCAACGC298050.020.1086984
CAACGCA299550.020.0238325
TATCAAC301850.019.934092
CGCGAAA600.004740291719.80997394-95
AACGCAG304000.019.7652346
GTAAGGT8700.019.6204624
TAAGGTG9900.019.1580285
Produced by FastQC (version 0.11.5)