##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939337_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2581678 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.038598539399565 33.0 31.0 34.0 30.0 34.0 2 32.082272460004695 34.0 31.0 34.0 30.0 34.0 3 32.129691619171716 34.0 31.0 34.0 30.0 34.0 4 35.618951705053846 37.0 35.0 37.0 33.0 37.0 5 35.66187301437282 37.0 35.0 37.0 35.0 37.0 6 35.765257324887145 37.0 35.0 37.0 35.0 37.0 7 35.711823085605566 37.0 35.0 37.0 35.0 37.0 8 35.69964805835585 37.0 35.0 37.0 33.0 37.0 9 37.33818431268346 39.0 37.0 39.0 34.0 39.0 10-11 37.2432044197611 39.0 37.0 39.0 34.0 39.0 12-13 37.157059091025296 39.0 37.0 39.0 34.0 39.0 14-15 38.384986431305535 40.0 38.0 41.0 34.0 41.0 16-17 38.328308565204495 40.0 38.0 41.0 33.5 41.0 18-19 38.29813187392076 40.0 38.0 41.0 33.5 41.0 20-21 38.286016691469655 40.0 38.0 41.0 34.0 41.0 22-23 38.28667866403168 40.0 38.0 41.0 34.0 41.0 24-25 38.20164114192397 40.0 38.0 41.0 33.5 41.0 26-27 38.10275468125769 40.0 38.0 41.0 33.0 41.0 28-29 37.96042031577912 40.0 38.0 41.0 33.0 41.0 30-31 37.75193091469966 40.0 37.5 41.0 33.0 41.0 32-33 37.49903647937504 40.0 37.0 41.0 32.5 41.0 34-35 37.20103669009071 40.0 36.0 41.0 31.5 41.0 36-37 36.94683593383838 40.0 36.0 41.0 31.0 41.0 38-39 36.35956343122574 39.5 35.5 40.5 30.0 41.0 40-41 36.314634706574566 39.0 35.0 40.5 30.0 41.0 42-43 36.44126068394277 39.5 35.0 41.0 30.0 41.0 44-45 36.281955960425734 40.0 35.0 41.0 30.0 41.0 46-47 35.91907801825015 39.5 35.0 41.0 28.5 41.0 48-49 35.59985559779338 39.0 35.0 41.0 27.0 41.0 50-51 34.42982548559503 38.0 34.0 40.0 24.5 40.5 52-53 34.520085773671234 38.5 34.5 39.5 24.5 40.5 54-55 35.00254621219223 39.0 35.0 40.5 25.5 41.0 56-57 34.918012819569284 39.0 35.0 41.0 25.0 41.0 58-59 34.70843265504064 38.5 35.0 40.5 25.0 41.0 60-61 34.40588485473401 38.0 34.0 40.0 23.5 41.0 62-63 34.037490926443965 37.5 34.0 40.0 22.5 41.0 64-65 33.64468884190825 37.0 34.0 40.0 22.0 41.0 66-67 33.28044860745608 36.5 33.0 39.5 20.5 41.0 68-69 32.877419066204226 36.0 33.0 39.0 19.5 41.0 70-71 32.461116762043915 35.5 33.0 39.0 18.0 40.0 72-73 32.02546580169952 35.0 32.0 38.0 15.5 40.0 74-75 31.576760541012476 35.0 32.0 37.0 11.5 39.5 76-77 31.127942562937747 35.0 31.0 37.0 7.5 39.0 78-79 30.745143468705237 35.0 31.0 36.0 6.0 39.0 80-81 30.351218277414922 35.0 31.0 36.0 2.0 37.5 82-83 29.936421195826902 34.0 30.5 35.0 2.0 37.0 84-85 29.53579919726627 34.0 30.0 35.0 2.0 36.5 86-87 29.12387563437423 34.0 29.5 35.0 2.0 36.0 88-89 28.701473808894836 34.0 29.0 35.0 2.0 36.0 90-91 28.304987879975737 34.0 29.0 35.0 2.0 35.5 92-93 27.874856779195547 34.0 28.0 35.0 2.0 35.0 94-95 27.41268275904276 34.0 27.0 35.0 2.0 35.0 96-97 26.8404483440615 33.5 25.5 35.0 2.0 35.0 98-99 26.09042084256828 33.0 24.5 35.0 2.0 35.0 100-101 23.446573120272937 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19031186014624524 1101 2 0.27443724585034346 1101 3 0.3043033701471245 1101 4 0.2248937745351398 1101 5 0.17834186499924698 1101 6 0.2636492794553149 1101 7 0.21507793326980362 1101 8 0.15555589174137197 1101 9 0.15878137614866716 1101 10-11 0.2893790659594373 1101 12-13 0.38391853882996685 1101 14-15 0.4411955764953319 1101 16-17 0.48878458205035713 1101 18-19 0.5333148656439377 1101 20-21 0.5242930717839727 1101 22-23 0.5552745348787553 1101 24-25 0.567290515290729 1101 26-27 0.5563213115010015 1101 28-29 0.5841633928976719 1101 30-31 0.643116402678551 1101 32-33 0.6794702999725146 1101 34-35 0.5869751162213817 1101 36-37 0.48502883227069304 1101 38-39 0.7011357784911425 1101 40-41 0.6979242410474811 1101 42-43 0.49429430849517075 1101 44-45 0.43833844582378134 1101 46-47 0.6413666286888215 1101 48-49 0.603691119524953 1101 50-51 0.5586831233301197 1101 52-53 0.6251795909039757 1101 54-55 0.5995039517494689 1101 56-57 0.5489166694955117 1101 58-59 0.5086425776709831 1101 60-61 0.5131300881597483 1101 62-63 0.6014751717686977 1101 64-65 0.38820623103451624 1101 66-67 0.2657686914950119 1101 68-69 0.35998527751765863 1101 70-71 0.40665379196892104 1101 72-73 0.40661741951600305 1101 74-75 0.33923323582415676 1101 76-77 0.34264397549865677 1101 78-79 0.2469719745366632 1101 80-81 0.24905433290132706 1101 82-83 0.32400857946623773 1101 84-85 0.40518858230202426 1101 86-87 0.43985262811919057 1101 88-89 0.3522057936887357 1101 90-91 0.24385741939751782 1101 92-93 0.10352847092520889 1101 94-95 0.15878969299964396 1101 96-97 0.45641783653123014 1101 98-99 0.3855717272436081 1101 100-101 -0.051648497748718825 1102 1 0.40406626372947585 1102 2 0.3172824461401689 1102 3 0.31961522949801946 1102 4 0.25338404706604933 1102 5 0.2721257396335801 1102 6 0.2964117860513866 1102 7 0.3098236996678452 1102 8 0.34279913131346973 1102 9 0.49876222988579144 1102 10-11 0.5268324069594144 1102 12-13 0.4268786815608223 1102 14-15 0.5768186904377259 1102 16-17 0.44469638180287774 1102 18-19 0.5037406048144604 1102 20-21 0.4946043454530482 1102 22-23 0.5109226824263189 1102 24-25 0.46215652769802773 1102 26-27 0.4623065027175315 1102 28-29 0.4746600663394318 1102 30-31 0.5118765543377037 1102 32-33 0.5215838367771752 1102 34-35 0.42338273855543207 1102 36-37 0.35654559936811125 1102 38-39 0.8089584653245936 1102 40-41 0.6515064770699937 1102 42-43 0.4846380941821735 1102 44-45 0.5949104635109919 1102 46-47 0.5576188984443249 1102 48-49 0.46954823529193135 1102 50-51 0.3236610295805846 1102 52-53 0.29832106225116917 1102 54-55 0.3891141661929609 1102 56-57 0.3068116412931161 1102 58-59 0.3417911764033974 1102 60-61 0.2263821789675191 1102 62-63 0.3230104631997648 1102 64-65 0.2196632204315776 1102 66-67 0.2509263314758243 1102 68-69 0.2031238528876642 1102 70-71 0.17461676772092716 1102 72-73 0.14897465337321592 1102 74-75 0.020555938110454974 1102 76-77 0.039860880374394725 1102 78-79 0.001260901487448507 1102 80-81 0.05938089534588187 1102 82-83 0.0922615344885429 1102 84-85 0.04889515456771676 1102 86-87 -0.16584290814595803 1102 88-89 -0.23647959673591856 1102 90-91 -0.06533736540161428 1102 92-93 -0.24559188077386196 1102 94-95 -0.33717520729899775 1102 96-97 -0.18267329594084103 1102 98-99 -0.24173604034716334 1102 100-101 -0.4943711054865112 1103 1 0.36301219553217834 1103 2 0.4266641670045104 1103 3 0.39917692724399245 1103 4 0.3705279260150789 1103 5 0.3552662007912275 1103 6 0.4013116471683986 1103 7 0.404060525036293 1103 8 0.43164085767002547 1103 9 0.515486416977005 1103 10-11 0.5986013300020119 1103 12-13 0.5513064043643467 1103 14-15 0.6292878737779262 1103 16-17 0.6321888920012171 1103 18-19 0.6596639062401337 1103 20-21 0.7092048661985331 1103 22-23 0.7267731313260484 1103 24-25 0.7451721369142703 1103 26-27 0.7873115835735831 1103 28-29 0.6462413264142697 1103 30-31 0.6128439643558465 1103 32-33 0.616380965051043 1103 34-35 0.5467962623854916 1103 36-37 0.555614760243607 1103 38-39 0.9795227089457228 1103 40-41 0.9099348753174539 1103 42-43 0.7528820301194301 1103 44-45 0.882781812103552 1103 46-47 0.863685346649838 1103 48-49 0.8968217446288733 1103 50-51 0.83196602219315 1103 52-53 0.9540431955871043 1103 54-55 1.0135471482929432 1103 56-57 0.9213816581191097 1103 58-59 1.0236497591807776 1103 60-61 1.1318874602403284 1103 62-63 1.051923314027718 1103 64-65 1.0446041566539677 1103 66-67 1.0063553341666989 1103 68-69 1.0047768121998715 1103 70-71 0.9358524093720106 1103 72-73 0.8496340940169844 1103 74-75 0.821330031417137 1103 76-77 0.7577243249956922 1103 78-79 0.8197582603814624 1103 80-81 0.8900759714798347 1103 82-83 0.7905890406466156 1103 84-85 0.8753333937101857 1103 86-87 0.7076937395709635 1103 88-89 0.6373803098815145 1103 90-91 0.5635308001895858 1103 92-93 0.6095131868212462 1103 94-95 0.6496171966239856 1103 96-97 0.6111432177241269 1103 98-99 0.9385246554652724 1103 100-101 0.6742851405103174 1104 1 0.23650443010406264 1104 2 0.2568571094063401 1104 3 0.22983041984282693 1104 4 0.24701734336859005 1104 5 0.2719841549096813 1104 6 0.2299619087028404 1104 7 0.27027952873674366 1104 8 0.2633072361171358 1104 9 0.37889343172993506 1104 10-11 0.4386713971063756 1104 12-13 0.47604296297963344 1104 14-15 0.5180612143771626 1104 16-17 0.48363079821018573 1104 18-19 0.5461903966222295 1104 20-21 0.5360718932388124 1104 22-23 0.5117848190946575 1104 24-25 0.5140935099597499 1104 26-27 0.47479960322756654 1104 28-29 0.5168776628871186 1104 30-31 0.5077678320181676 1104 32-33 0.4892796596959812 1104 34-35 0.5203063311055658 1104 36-37 0.44242327901572054 1104 38-39 0.88346337046098 1104 40-41 0.7971817643044758 1104 42-43 0.7054242617970985 1104 44-45 0.715682786057215 1104 46-47 0.6864230200346313 1104 48-49 0.6451053656497905 1104 50-51 0.606450152776624 1104 52-53 0.632788425313116 1104 54-55 0.6962297756506715 1104 56-57 0.6582616126627698 1104 58-59 0.64538053350919 1104 60-61 0.6517838057907497 1104 62-63 0.6125961140304526 1104 64-65 0.6405330299595633 1104 66-67 0.744512801580612 1104 68-69 0.8835197517976425 1104 70-71 0.9096177282110531 1104 72-73 0.9555660490778237 1104 74-75 0.8721943204017428 1104 76-77 0.8225509126992208 1104 78-79 0.7890281656981841 1104 80-81 0.6958859907367447 1104 82-83 0.741909029084173 1104 84-85 0.6157460964564088 1104 86-87 0.5997397996397638 1104 88-89 0.6281952727498812 1104 90-91 0.6473520179932244 1104 92-93 0.6683275567808202 1104 94-95 0.8573832198127072 1104 96-97 0.6756010406849526 1104 98-99 0.31177579543520295 1104 100-101 -0.007308593987364276 1105 1 0.24842404317973887 1105 2 0.28653085357134955 1105 3 0.27316565248762714 1105 4 0.3270874840896738 1105 5 0.2524480074327542 1105 6 0.30978060352310877 1105 7 0.30534311222833566 1105 8 0.2840745475244617 1105 9 0.35837652360048367 1105 10-11 0.4247860053486221 1105 12-13 0.36716662727103255 1105 14-15 0.4715962014147479 1105 16-17 0.43217023116579867 1105 18-19 0.4897122066434676 1105 20-21 0.5772039767105142 1105 22-23 0.5350943059034776 1105 24-25 0.4909186083963917 1105 26-27 0.5891777733443604 1105 28-29 0.6286887215369248 1105 30-31 0.6493859167997442 1105 32-33 0.5464761300002507 1105 34-35 0.577596289934128 1105 36-37 0.5859277293648759 1105 38-39 1.0405858666671435 1105 40-41 0.8312366969797083 1105 42-43 0.7199971765034192 1105 44-45 0.7184328778514768 1105 46-47 0.863394103060962 1105 48-49 0.7325674424122113 1105 50-51 0.7119319688242243 1105 52-53 0.6685226760156198 1105 54-55 0.9051098126707373 1105 56-57 0.8456391153271099 1105 58-59 0.8537646295472783 1105 60-61 0.9299408376656331 1105 62-63 0.8259582133309067 1105 64-65 0.937849040460172 1105 66-67 0.87612014213272 1105 68-69 0.7763769505095865 1105 70-71 0.8149438285214572 1105 72-73 0.833153513413599 1105 74-75 0.7935765534463961 1105 76-77 0.8474333699946861 1105 78-79 0.8445038737130197 1105 80-81 0.8036740018587487 1105 82-83 0.8676543958850047 1105 84-85 0.953781137971049 1105 86-87 0.8819138839605003 1105 88-89 0.7314878412636752 1105 90-91 0.8353135632785964 1105 92-93 0.8922903511284836 1105 94-95 0.9413229687178237 1105 96-97 0.909752259589375 1105 98-99 0.9056605164388642 1105 100-101 0.8053067787581618 1106 1 0.18411598024736264 1106 2 0.19337985692770587 1106 3 0.2179729400191306 1106 4 0.130893008109787 1106 5 0.13464049977149273 1106 6 0.1364657709824897 1106 7 0.15957170584655245 1106 8 0.08957779134660626 1106 9 0.12178996932365749 1106 10-11 0.23207625755795647 1106 12-13 0.2071310602390426 1106 14-15 0.25271055263795716 1106 16-17 0.1938738375836948 1106 18-19 0.3020068243473375 1106 20-21 0.35431122442079044 1106 22-23 0.29448605524246574 1106 24-25 0.3816345742342264 1106 26-27 0.3619019656042042 1106 28-29 0.3087385351686365 1106 30-31 0.3238424560137645 1106 32-33 0.27930415424915367 1106 34-35 0.2137509941564133 1106 36-37 0.28849755872722227 1106 38-39 0.8530004427194982 1106 40-41 0.7652022231763809 1106 42-43 0.5150229860986002 1106 44-45 0.3602320552092735 1106 46-47 0.24466303978478265 1106 48-49 0.3426325661919165 1106 50-51 0.29780879015059725 1106 52-53 0.34207544655880184 1106 54-55 0.24133426587090412 1106 56-57 0.3152933632129944 1106 58-59 0.3611646992962605 1106 60-61 0.37277388285159674 1106 62-63 0.14887223658551108 1106 64-65 0.2817112540750699 1106 66-67 0.15971453945950032 1106 68-69 0.2984873764977607 1106 70-71 0.09843731243343967 1106 72-73 -0.1322598976824274 1106 74-75 -0.06595395183795816 1106 76-77 0.12141477521398869 1106 78-79 0.1631458957408327 1106 80-81 0.1677804852983158 1106 82-83 0.17526940131405055 1106 84-85 0.31308780345050025 1106 86-87 0.43024572090619273 1106 88-89 0.4002976813109669 1106 90-91 0.5281996622083724 1106 92-93 0.5618802914461156 1106 94-95 0.6694674233030753 1106 96-97 0.6317855164956363 1106 98-99 0.7692948339253256 1106 100-101 0.6898403139183209 1107 1 0.049227006712804666 1107 2 0.10136144412864923 1107 3 0.12718367698060717 1107 4 0.08699140002607209 1107 5 0.06888674453995947 1107 6 0.024250217892699766 1107 7 0.07099516473675038 1107 8 0.10644110147072894 1107 9 0.15670851626769888 1107 10-11 0.31188045792845287 1107 12-13 0.26908380429656376 1107 14-15 0.26471420239099785 1107 16-17 0.28052881683893105 1107 18-19 0.22000788268108806 1107 20-21 0.2404506800019277 1107 22-23 0.08913090225330933 1107 24-25 0.15381198945514285 1107 26-27 0.2779143501594632 1107 28-29 0.3121882142379988 1107 30-31 0.3195026567501813 1107 32-33 0.3681434649600135 1107 34-35 0.3674799645049305 1107 36-37 0.20565321186187902 1107 38-39 0.6735176855944687 1107 40-41 0.5100677666843936 1107 42-43 0.17776072673743215 1107 44-45 0.35685343118510815 1107 46-47 0.5320628746468614 1107 48-49 0.5486454318079765 1107 50-51 0.5277461497261839 1107 52-53 0.5557612318432277 1107 54-55 0.640400061580948 1107 56-57 0.4952798935107552 1107 58-59 0.5248718957291558 1107 60-61 0.5032115456532225 1107 62-63 0.6964261213091447 1107 64-65 0.5986749560665814 1107 66-67 0.4547754646261595 1107 68-69 0.5004203734039905 1107 70-71 0.49556427541778447 1107 72-73 0.49270053309568596 1107 74-75 0.44680212431475397 1107 76-77 0.5553014073231388 1107 78-79 0.6665759380662983 1107 80-81 0.5639585755815979 1107 82-83 0.5556203548790677 1107 84-85 0.472853929042067 1107 86-87 0.746520559612815 1107 88-89 0.6659272282226745 1107 90-91 0.5615923000855219 1107 92-93 0.6377067499475224 1107 94-95 0.7386607632135345 1107 96-97 0.7019069882893092 1107 98-99 0.802503643489132 1107 100-101 0.5730732159530518 1108 1 0.13750078432139645 1108 2 0.17084946561337233 1108 3 0.25206835058315136 1108 4 0.10906793950901061 1108 5 0.19756797302813567 1108 6 0.14653397624184805 1108 7 0.2219684974883549 1108 8 0.24499285579254604 1108 9 0.3458644828591133 1108 10-11 0.3730703755167326 1108 12-13 0.41803207467804526 1108 14-15 0.5004217481768478 1108 16-17 0.4483716138664491 1108 18-19 0.47061166929385223 1108 20-21 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2316 88-89 -0.9276206934423108 2316 90-91 -0.9428504453686841 2316 92-93 -1.0592625805094151 2316 94-95 -0.9760173029772012 2316 96-97 -1.054087450001795 2316 98-99 -1.2331605521485365 2316 100-101 -0.48400198293509433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8658.0 3 1756.0 4 1728.0 5 2047.0 6 2449.0 7 2850.0 8 3628.0 9 4582.0 10 5471.0 11 7211.0 12 10060.0 13 13972.0 14 14669.0 15 16714.0 16 22251.0 17 29421.0 18 25016.0 19 15456.0 20 15044.0 21 15294.0 22 16447.0 23 18131.0 24 20724.0 25 23215.0 26 26849.0 27 31386.0 28 37453.0 29 44504.0 30 54190.0 31 66373.0 32 82877.0 33 108203.0 34 149838.0 35 223390.0 36 344151.0 37 536775.0 38 504000.0 39 74887.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70570958829179 19.008560808311863 14.168223224358755 22.117506379037597 2 15.1865248400337 20.960290638270294 40.257529349630126 23.59565517206588 3 19.309994776803418 25.967319708525455 30.040520519091057 24.682164995580074 4 12.273354460718007 16.54200474686922 37.53380155136846 33.65083924104431 5 13.07668508870645 38.54665284204961 33.50069349527441 14.87596857396953 6 30.707435521997283 36.3549997208073 17.799852958511963 15.137711798683451 7 28.220363994860463 34.13451451127676 20.505591940562578 17.13952955330019 8 26.731210072494964 33.96786793426192 20.060548231068495 19.240373762174617 9 27.705975972912412 16.752087950110297 20.69400897670177 34.84792710027552 10-11 25.0808990886284 26.863539376578156 26.845330173192988 21.210231361600453 12-13 26.52016961795628 24.65227019817007 27.509691720586044 21.31786846328761 14-15 23.131129797879208 25.462743802883793 26.974223262382353 24.431903136854643 16-17 21.420897758715057 29.25340330235641 27.393023765775165 21.932675173153363 18-19 21.364301311165807 27.186360788099726 30.619824364640124 20.829513536094343 20-21 22.44435826967706 26.1073786314727 30.2971988393953 21.151064259454937 22-23 21.88096363748883 26.309116943699866 30.24005189849917 21.569867520312137 24-25 21.80054883493853 26.52273364972906 30.56298853081676 21.11372898451565 26-27 21.55038567189012 26.5255035831721 30.647039069273497 21.277071675664285 28-29 21.095644376811837 26.942989500541938 30.880698523099642 21.080667599546583 30-31 21.173451127961023 26.751444709959117 30.541879470133672 21.53322469194619 32-33 21.069786354682346 26.910801149571125 30.313245326811806 21.70616716893473 34-35 21.898871801091193 26.702735291510987 30.049821270032297 21.348571637365524 36-37 22.116680858482944 26.256189785316998 30.193329119894642 21.433800236305416 38-39 21.622613160535124 26.605753897160632 30.38308868240976 21.38854425989448 40-41 21.49399630990999 26.64790760693034 30.51030685008455 21.347789233075126 42-43 21.406066425985195 26.895677869437858 29.758661605053728 21.939594099523223 44-45 21.403341658393163 27.067067218972806 29.606122917105633 21.923468205528394 46-47 21.851000974358495 27.23804776535704 28.91943876328069 21.99151249700377 48-49 21.89437147425426 27.32930530878484 28.766057338931393 22.010265878029507 50-51 21.806581486009947 27.588516254863304 28.77759894526869 21.827303313858057 52-53 22.06586975489437 27.686716848550702 28.50266540991428 21.74474798664065 54-55 21.319238288602634 27.521737599407942 28.77668477217276 22.38233933981666 56-57 21.773186332758325 27.934297044106003 28.305111115865056 21.987405507270616 58-59 21.968162652400576 27.11034096387112 28.593174733215736 22.328321650512567 60-61 21.57565506351012 27.447221330256504 29.168257302866724 21.808866303366656 62-63 21.807919036633695 27.324377270731958 29.029235819891845 21.8384678727425 64-65 21.442533548558824 27.414068057538053 28.941712443592817 22.201685950310306 66-67 21.378198623109032 27.7213386576179 28.52426235690466 22.376200362368408 68-69 21.4695587781669 27.51862710658782 28.844267950088987 22.167546165156292 70-71 21.551797616151788 27.65297008027244 28.553986864509852 22.24124543906592 72-73 21.603956330917544 27.431527474141898 28.512999831682755 22.451516363257802 74-75 21.314955168975484 28.027972006199047 28.31507893202606 22.3419938927994 76-77 21.56655034531624 27.44656639956919 28.50277749081676 22.48410576429781 78-79 21.528324741531055 27.530538814060897 28.501291542309076 22.439844902098972 80-81 21.459768028899227 28.00045283908515 28.315670080271953 22.224109051743675 82-83 21.556413269316067 27.664485203140686 28.33870917537566 22.44039235216759 84-85 21.268890897467674 27.596517160703122 28.311084410761193 22.823507531068017 86-87 21.538676737461053 27.33380621243296 28.409593194276265 22.71792385582972 88-89 21.511520332063554 27.194941221723518 28.29939419102799 22.994144255184942 90-91 21.194098569672192 27.236358838659914 28.691672916477746 22.87786967519015 92-93 21.41620564651411 27.22223779065287 28.65048561827298 22.711070944560042 94-95 21.08737842916972 26.912522712584636 28.766932150355608 23.233166707890035 96-97 21.243869157845495 26.645762839838 28.71392231451405 23.396445687802455 98-99 21.16370229888502 26.85242013603621 28.318310090474156 23.665567474604615 100-101 21.215581425935422 26.247263518587836 29.125968031515548 23.41118702396119 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4674.0 1 2845.5 2 1256.5 3 1551.5 4 1380.5 5 1310.5 6 2247.0 7 4363.0 8 6529.5 9 7089.5 10 6032.0 11 5165.5 12 5168.5 13 5325.0 14 5587.0 15 5814.5 16 5838.0 17 5876.5 18 5980.5 19 6234.5 20 6665.0 21 7379.5 22 8611.0 23 9996.5 24 11413.0 25 13621.5 26 16971.0 27 20538.5 28 22953.5 29 25802.0 30 30139.0 31 35542.0 32 43345.0 33 49380.5 34 53564.0 35 57119.0 36 62134.5 37 68883.5 38 71976.0 39 72751.0 40 73325.0 41 76436.0 42 80439.0 43 84031.0 44 87707.0 45 91892.0 46 91527.5 47 92997.0 48 113401.5 49 114058.0 50 93504.5 51 84077.5 52 79007.5 53 78676.5 54 78314.5 55 73130.0 56 64951.0 57 58025.0 58 53350.0 59 47007.0 60 38879.0 61 32220.0 62 27582.5 63 21833.5 64 15919.5 65 12438.0 66 9452.5 67 7611.0 68 5629.0 69 3582.0 70 2765.5 71 2307.5 72 2018.5 73 1783.0 74 1416.0 75 1190.0 76 909.0 77 509.0 78 292.5 79 201.5 80 130.0 81 108.5 82 95.0 83 62.0 84 38.5 85 29.5 86 20.5 87 15.5 88 15.5 89 12.5 90 7.5 91 6.0 92 4.5 93 3.5 94 3.5 95 4.0 96 2.0 97 1.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.295234339836339 2 0.1831754386100823 3 0.18251695215282462 4 0.20308497031775458 5 0.21679698242770784 6 0.10899887592488297 7 0.12530609936638107 8 0.13820468702913377 9 0.14219434027016536 10-11 0.12826928842404048 12-13 0.11850819505763308 14-15 0.13334350759467292 16-17 0.10932811915351179 18-19 0.13760430231810472 20-21 0.16378882261846753 22-23 0.1680883518393851 24-25 0.19161955906197442 26-27 0.17130331513070182 28-29 0.16866936930167123 30-31 0.15821105498052043 32-33 0.16923101951521452 34-35 0.35178670616552493 36-37 0.469539578522186 38-39 0.4369638661366754 40-41 0.42642808282055317 42-43 0.449881821048171 44-45 0.4542200847665743 46-47 0.45636984937703307 48-49 0.4685905833337852 50-51 0.46187014802000864 52-53 0.46837754359761363 54-55 0.45123752846017207 56-57 0.41496267156477296 58-59 0.40487233496973674 60-61 0.36147033053696087 62-63 0.4656661287736116 64-65 0.46365193490435286 66-67 0.4723090950924167 68-69 0.48439425830796873 70-71 0.47655052256710556 72-73 0.46967514926338605 74-75 0.47457506319533266 76-77 0.4509663869777718 78-79 0.4377579233351332 80-81 0.4349303050186739 82-83 0.44691863199051163 84-85 0.448971560357256 86-87 0.44955257781954217 88-89 0.4573963135604053 90-91 0.4751560806576188 92-93 0.4793781408835649 94-95 0.4780805352177925 96-97 0.47612444309476243 98-99 0.4731418867883601 100-101 0.4559825044021757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2581678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.486447551230015 #Duplication Level Percentage of deduplicated Percentage of total 1 68.25287575974082 26.268107231478833 2 14.631280936337902 11.262120527273604 3 6.211073000429232 7.171264058036414 4 3.1977721918872493 4.9228356697540185 5 1.913606927669763 3.6823966327716375 6 1.2574777443529959 2.903751075292836 7 0.8603439857903747 2.3178108579596204 8 0.5822394357508864 1.7926662005027398 9 0.44014794037496635 1.5245757559820752 >10 2.301450384626578 16.113525830953233 >50 0.20012889977198028 5.397382332398704 >100 0.1377745272012033 9.794527527362206 >500 0.00928613487645101 2.3787046592757473 >1k 0.004138386194939924 2.7975506024159604 >5k 2.0187249731414266E-4 0.5710864598502677 >10k+ 2.0187249731414266E-4 1.1016945786921701 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16308 0.6316821849975094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12052 0.46682816369818386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8718 0.3376873490807142 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5983 0.23174849845720497 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4522 0.1751573976305333 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3932 0.1523040441139445 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3202 0.12402786094935153 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2861 0.11081939730671292 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2796 0.10830165497013958 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2593 0.10043855198053359 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2584 0.1000899415031619 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9367248742871884E-5 0.0 14-15 0.0 0.0 0.0 3.873449748574377E-5 0.0 16-17 0.0 0.0 0.0 3.873449748574377E-5 0.0 18-19 0.0 0.0 0.0 7.746899497148754E-5 0.0 20-21 0.0 0.0 0.0 1.5493798994297507E-4 0.0 22-23 0.0 0.0 0.0 4.260794723431815E-4 0.0 24-25 0.0 0.0 0.0 9.102606909149785E-4 0.0 26-27 0.0 0.0 0.0 0.0015881143969154947 0.0 28-29 0.0 0.0 0.0 0.004357630967146173 0.0 30-31 0.0 0.0 0.0 0.012763016921552572 0.0 32-33 0.0 0.0 0.0 0.027985674433449875 0.0 34-35 0.0 0.0 0.0 0.051749288640953676 0.0 36-37 0.0 0.0 0.0 0.09278848872709919 0.0 38-39 0.0 0.0 0.0 0.15617749386251886 0.0 40-41 0.0 0.0 0.0 0.2281461901910308 0.0 42-43 0.0 0.0 0.0 0.3017030009164582 0.0 44-45 0.0 0.0 0.0 0.3896496774578394 0.0 46-47 0.0 0.0 0.0 0.5025607376287825 0.0 48-49 0.0 0.0 0.0 0.625678337887219 0.0 50-51 0.0 0.0 0.0 0.7629727642254378 0.0 52-53 0.0 0.0 0.0 0.9015647962294291 0.0 54-55 0.0 0.0 0.0 1.0494724748787416 0.0 56-57 0.0 0.0 0.0 1.2272831855870483 3.873449748574377E-5 58-59 0.0 0.0 0.0 1.4319369030529756 3.873449748574377E-5 60-61 0.0 0.0 0.0 1.6668228958065257 3.873449748574377E-5 62-63 0.0 0.0 0.0 1.9016314195651045 3.873449748574377E-5 64-65 0.0 0.0 0.0 2.1401584550823145 3.873449748574377E-5 66-67 0.0 0.0 0.0 2.413740210824123 3.873449748574377E-5 68-69 0.0 0.0 0.0 2.7270635609863043 3.873449748574377E-5 70-71 0.0 0.0 0.0 3.0822589029305747 3.873449748574377E-5 72-73 0.0 0.0 0.0 3.4455497548493654 3.873449748574377E-5 74-75 0.0 0.0 0.0 3.807175023376269 3.873449748574377E-5 76-77 0.0 0.0 0.0 4.204009950117714 3.873449748574377E-5 78-79 0.0 0.0 0.0 4.665279713426694 3.873449748574377E-5 80-81 0.0 0.0 0.0 5.173611891180853 3.873449748574377E-5 82-83 0.0 0.0 0.0 5.693719356170677 3.873449748574377E-5 84-85 0.0 0.0 0.0 6.234452941071659 3.873449748574377E-5 86-87 0.0 0.0 0.0 6.8010030685468905 3.873449748574377E-5 88-89 0.0 0.0 0.0 7.406461998746551 3.873449748574377E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17210 0.0 27.96055 1 TACATGG 17570 0.0 26.825056 2 ACATGGG 17425 0.0 25.850967 3 GTATCAA 25290 0.0 23.79354 1 CATGGGA 11235 0.0 22.5369 4 CATGGGG 5630 0.0 22.402641 4 GAGTACT 12600 0.0 22.205221 12-13 AGTACTT 12875 0.0 22.006943 12-13 TATAACG 200 5.483889E-7 21.337252 2 ATGGGAT 4365 0.0 20.747946 5 GGTATCA 19045 0.0 20.731512 1 GTACTTT 13675 0.0 20.41105 14-15 ATCAACG 29770 0.0 20.132338 3 TCAACGC 29805 0.0 20.108698 4 CAACGCA 29955 0.0 20.023832 5 TATCAAC 30185 0.0 19.93409 2 CGCGAAA 60 0.0047402917 19.809973 94-95 AACGCAG 30400 0.0 19.765234 6 GTAAGGT 870 0.0 19.620462 4 TAAGGTG 990 0.0 19.158028 5 >>END_MODULE