FastQCFastQC Report
Fri 27 May 2016
SRR939337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581678
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166190.6437286137155757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131810.5105594113595886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93120.360695640587246No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62980.24394986516521425No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45600.1766293085349916No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38950.15087086770697197No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30410.1177916068541468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.11724932388934639No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.10489301919139413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27080.10489301919139413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1606.134542E-826.7693121
GTACATG165850.026.0833341
TACATGG167750.025.1735762
ACATGGG169750.023.9266263
GAGTACT136300.022.94160712-13
GTATCAA266350.022.4593561
GGTATCA203450.022.4557861
GTACTTT145200.021.40469214-15
AGTACTT141050.021.36165412-13
CATGGGA110500.020.9973284
CATGGGG56150.020.8720264
AGTACAT130300.019.1539692
ATGGGAG39600.019.0510715
ATCAACG311950.019.0126423
ACTTTTT165850.018.96828816-17
ATGGGAT46050.018.9585725
TCAACGC313500.018.918644
CAACGCA315200.018.8467125
TATCAAC316000.018.8440442
AACGCAG320350.018.5585386
Produced by FastQC (version 0.11.5)