##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939337_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2581678 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.303199314554334 33.0 31.0 34.0 31.0 34.0 2 32.50005267891658 34.0 31.0 34.0 31.0 34.0 3 32.580149809542476 34.0 31.0 34.0 31.0 34.0 4 36.11435314551234 37.0 35.0 37.0 35.0 37.0 5 36.06568557349135 37.0 35.0 37.0 35.0 37.0 6 36.10815949936437 37.0 35.0 37.0 35.0 37.0 7 36.04916569765865 37.0 35.0 37.0 35.0 37.0 8 36.02476180220771 37.0 35.0 37.0 35.0 37.0 9 37.71401739488813 39.0 37.0 39.0 35.0 39.0 10-11 37.64188117185799 39.0 37.0 39.0 35.0 39.0 12-13 37.567752252604706 39.0 37.0 39.0 35.0 39.0 14-15 38.82719591676421 40.0 38.0 41.0 35.0 41.0 16-17 38.83504914245696 40.0 38.0 41.0 34.5 41.0 18-19 38.972021878793555 40.0 38.5 41.0 35.0 41.0 20-21 38.96539614932614 40.0 38.0 41.0 35.0 41.0 22-23 38.88670391892405 40.0 38.0 41.0 35.0 41.0 24-25 38.81508247736549 40.0 38.0 41.0 35.0 41.0 26-27 38.64031397408972 40.0 38.0 41.0 34.0 41.0 28-29 38.46857044139509 40.0 38.0 41.0 34.0 41.0 30-31 38.23904665880098 40.0 37.5 41.0 34.0 41.0 32-33 38.136007085314276 40.0 37.0 41.0 33.5 41.0 34-35 38.01438192524397 40.0 37.0 41.0 33.0 41.0 36-37 37.833304928035176 40.0 37.0 41.0 33.0 41.0 38-39 37.68210965891176 40.0 37.0 41.0 33.0 41.0 40-41 37.47082808158105 40.0 37.0 41.0 33.0 41.0 42-43 37.24757386474998 40.0 36.0 41.0 32.0 41.0 44-45 36.93855934008812 40.0 36.0 41.0 31.0 41.0 46-47 36.53360237024137 40.0 36.0 41.0 30.5 41.0 48-49 36.13203137649234 39.0 35.0 41.0 29.5 41.0 50-51 35.85402497910273 39.0 35.0 41.0 28.5 41.0 52-53 35.58892859605265 39.0 35.0 40.5 28.0 41.0 54-55 35.33569755794487 39.0 35.0 40.0 27.0 41.0 56-57 35.028738092047114 38.0 34.5 40.0 26.5 41.0 58-59 34.72307487610771 38.0 34.0 40.0 26.0 41.0 60-61 34.37910924600202 37.5 34.0 40.0 26.0 41.0 62-63 34.04187005505722 37.0 34.0 40.0 25.0 41.0 64-65 33.74398414519549 36.5 33.5 39.5 24.5 41.0 66-67 33.33615714275754 36.0 33.0 39.0 23.0 40.5 68-69 32.884791209438205 35.5 32.5 39.0 22.0 40.0 70-71 32.406810028206465 35.0 32.0 38.0 21.5 40.0 72-73 31.929203409565407 35.0 31.5 37.5 19.5 39.5 74-75 31.424098396469272 35.0 31.0 37.0 18.0 39.0 76-77 29.489935421845793 33.0 28.5 35.0 12.0 37.5 78-79 30.435449347284983 34.0 30.5 36.0 12.0 38.0 80-81 30.451057994064325 34.0 31.0 35.5 10.0 37.0 82-83 30.107611212552456 34.0 30.5 35.0 7.0 37.0 84-85 29.681959562734008 34.0 30.0 35.0 2.0 36.0 86-87 29.10561948469174 34.0 29.0 35.0 2.0 36.0 88-89 28.525233975732064 34.0 29.0 35.0 2.0 35.5 90-91 28.065877115581415 33.0 28.0 35.0 2.0 35.0 92-93 27.726569502470873 33.0 27.0 35.0 2.0 35.0 94-95 27.23931702559343 33.0 26.5 35.0 2.0 35.0 96-97 26.550732120736978 33.0 25.0 34.5 2.0 35.0 98-99 25.602714010035335 32.0 23.0 34.0 2.0 35.0 100-101 21.40734805037654 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21417104632349293 1101 2 0.23083451272081845 1101 3 0.2546970129058437 1101 4 0.15018337311003194 1101 5 0.22165194648701458 1101 6 0.20127332260862119 1101 7 0.192628666854624 1101 8 0.21159453253114435 1101 9 0.31639044594641774 1101 10-11 0.25725729908620565 1101 12-13 0.3070921940967253 1101 14-15 0.3034753359973834 1101 16-17 0.30068526866004675 1101 18-19 0.31450839211428416 1101 20-21 0.31442634786677104 1101 22-23 0.2923263343915039 1101 24-25 0.31835752849277554 1101 26-27 0.329051638021177 1101 28-29 0.3764309614903354 1101 30-31 0.44216411921316023 1101 32-33 0.5455848867315538 1101 34-35 0.4608688698082801 1101 36-37 0.45002625093704296 1101 38-39 0.5179985842249337 1101 40-41 0.5915758267435649 1101 42-43 0.5106516102018617 1101 44-45 0.548013321387991 1101 46-47 0.6053916934819981 1101 48-49 0.5275016141909177 1101 50-51 0.629113939525034 1101 52-53 0.5664046877300137 1101 54-55 0.6378074056328416 1101 56-57 0.6621572291320561 1101 58-59 0.5400582327383177 1101 60-61 0.5664416048187846 1101 62-63 0.5041129986247697 1101 64-65 0.48631610727816366 1101 66-67 0.5089681745310628 1101 68-69 0.5294529130535395 1101 70-71 0.5101045982455048 1101 72-73 0.5029256562341935 1101 74-75 0.56671625066204 1101 76-77 0.5782676200454162 1101 78-79 0.6385809327251728 1101 80-81 0.7174817494355246 1101 82-83 0.6949389367622665 1101 84-85 0.640469725764568 1101 86-87 0.6560115922006879 1101 88-89 0.78699760000816 1101 90-91 0.8193184471200183 1101 92-93 0.8202578149820319 1101 94-95 0.6886555352097758 1101 96-97 0.7123838668724787 1101 98-99 0.7036074521921734 1101 100-101 0.5096839758478211 1102 1 0.2461803699201397 1102 2 0.26466421934192397 1102 3 0.2918055838155098 1102 4 0.17171147229210248 1102 5 0.18768449971251755 1102 6 0.18804492615964108 1102 7 0.24294712895719783 1102 8 0.2259567097718147 1102 9 0.31629276871853307 1102 10-11 0.22872238500973907 1102 12-13 0.31192740765310134 1102 14-15 0.357132470613017 1102 16-17 0.30262192955638056 1102 18-19 0.3325520660448049 1102 20-21 0.2430776161254542 1102 22-23 0.2542751400775387 1102 24-25 0.3485746085007335 1102 26-27 0.29917738219001677 1102 28-29 0.35607775529256 1102 30-31 0.3620045008869255 1102 32-33 0.37189131011577814 1102 34-35 0.49528282742627994 1102 36-37 0.47557390158986124 1102 38-39 0.46982539639505916 1102 40-41 0.45523262940923814 1102 42-43 0.5204595866921764 1102 44-45 0.5429078738603152 1102 46-47 0.6231583608990476 1102 48-49 0.6323921718172372 1102 50-51 0.5746137664913604 1102 52-53 0.5269358381807976 1102 54-55 0.47252399095904707 1102 56-57 0.31439882612421144 1102 58-59 0.2482158456289696 1102 60-61 0.4281298870235517 1102 62-63 0.5910245553288789 1102 64-65 0.3688851862544027 1102 66-67 0.5132278173910692 1102 68-69 0.42594490790869344 1102 70-71 0.3430572701946275 1102 72-73 0.567364487205662 1102 74-75 0.5862465452924255 1102 76-77 0.484409342835157 1102 78-79 0.4369181877737667 1102 80-81 0.6094010382842576 1102 82-83 0.6251123100262532 1102 84-85 0.519906360906166 1102 86-87 0.33523576029403657 1102 88-89 0.4477463684547516 1102 90-91 0.6044358906322458 1102 92-93 0.5662704092396034 1102 94-95 0.5779197013648414 1102 96-97 0.3694108849278841 1102 98-99 0.3257906946716922 1102 100-101 0.28413247152312593 1103 1 0.18446308941218348 1103 2 0.15930492111239403 1103 3 0.17006939989339998 1103 4 0.11336164417718209 1103 5 0.13902567666042387 1103 6 0.18474922559693852 1103 7 0.219124957791891 1103 8 0.15841889073443838 1103 9 0.19986020961624718 1103 10-11 0.25026202150970533 1103 12-13 0.26573707296274307 1103 14-15 0.32153388942094097 1103 16-17 0.3001251724761431 1103 18-19 0.1948248973081661 1103 20-21 0.30660925999745103 1103 22-23 0.22884531468241676 1103 24-25 0.26611024868670796 1103 26-27 0.24436039255872544 1103 28-29 0.20119325883451467 1103 30-31 0.32691259655953786 1103 32-33 0.4101305277127878 1103 34-35 0.3658931462410777 1103 36-37 0.4185134463526694 1103 38-39 0.3493037342920857 1103 40-41 0.32506250619519506 1103 42-43 0.4247037638057449 1103 44-45 0.48207312208220543 1103 46-47 0.521763539127214 1103 48-49 0.487053861937234 1103 50-51 0.4641703251533613 1103 52-53 0.36407420989510086 1103 54-55 0.47330856529225684 1103 56-57 0.6560720262145239 1103 58-59 0.5189105198024038 1103 60-61 0.6681585426760037 1103 62-63 0.5614652430495113 1103 64-65 0.7007806028902337 1103 66-67 0.6959680836542219 1103 68-69 0.5747562317750479 1103 70-71 0.5611910990037714 1103 72-73 0.6433377481911116 1103 74-75 0.6582878247590429 1103 76-77 0.601689399996193 1103 78-79 0.6680136466342397 1103 80-81 0.8867644064579281 1103 82-83 0.8555160774857065 1103 84-85 0.7088801143871599 1103 86-87 0.8496357140122299 1103 88-89 1.0907743445309634 1103 90-91 1.2263346431646909 1103 92-93 1.0993569250420983 1103 94-95 1.079773415536355 1103 96-97 0.654258889381758 1103 98-99 0.5483253337130272 1103 100-101 0.4336876101750846 1104 1 0.19485756670484022 1104 2 0.15227747329440433 1104 3 0.17407604234722385 1104 4 0.09338433267591739 1104 5 0.1497432964562222 1104 6 0.13788743158982442 1104 7 0.1718422851004462 1104 8 0.2321538067687854 1104 9 0.26374234483082404 1104 10-11 0.2477729045777366 1104 12-13 0.21320026906931133 1104 14-15 0.2742186680565979 1104 16-17 0.2254207387698557 1104 18-19 0.20031534449807964 1104 20-21 0.2416574365414519 1104 22-23 0.25871607030391885 1104 24-25 0.2579472038187731 1104 26-27 0.3351193464642108 1104 28-29 0.31685113603710846 1104 30-31 0.25478338500019504 1104 32-33 0.31453732950182456 1104 34-35 0.3482587982126333 1104 36-37 0.42116692957460344 1104 38-39 0.4132305901209392 1104 40-41 0.42612397161398263 1104 42-43 0.33233035965996294 1104 44-45 0.305117528389907 1104 46-47 0.1993902125212017 1104 48-49 0.20156359146587732 1104 50-51 0.2232676792257564 1104 52-53 0.33664541620301236 1104 54-55 0.33481035564959427 1104 56-57 0.19319843101434486 1104 58-59 0.13762508705562482 1104 60-61 0.2122889530363281 1104 62-63 0.28719885497303466 1104 64-65 0.28587266153698465 1104 66-67 0.3295363865483836 1104 68-69 0.3890496433646504 1104 70-71 0.25631579468269905 1104 72-73 0.30284283525114475 1104 74-75 0.33784370578128886 1104 76-77 0.2464703149691445 1104 78-79 0.41513543330933445 1104 80-81 0.5532331062655729 1104 82-83 0.5918692123737195 1104 84-85 0.33876022887914914 1104 86-87 0.41855081174570685 1104 88-89 0.553754151190045 1104 90-91 0.6792668277793226 1104 92-93 0.6290627733984628 1104 94-95 0.6032799589015561 1104 96-97 0.5767969248953371 1104 98-99 0.585650005913628 1104 100-101 0.02284293334373544 1105 1 0.06815385564485155 1105 2 0.04926424084526815 1105 3 0.11102255075067546 1105 4 0.12080809652043456 1105 5 0.08117832618921739 1105 6 0.1625348642958997 1105 7 0.036232836364739285 1105 8 0.033072253323148004 1105 9 0.0782970831437595 1105 10-11 0.14644819999191583 1105 12-13 0.16608405119099956 1105 14-15 0.16294794874929153 1105 16-17 0.06545153437631512 1105 18-19 0.1382786709309869 1105 20-21 0.2238827136489121 1105 22-23 0.1844444771048046 1105 24-25 0.15363312547226116 1105 26-27 0.15843229130580738 1105 28-29 0.20091963149918257 1105 30-31 0.20530901467060403 1105 32-33 0.23336470189911296 1105 34-35 0.28686229513524353 1105 36-37 0.13933734525272712 1105 38-39 0.3236565936933218 1105 40-41 0.2754739917800464 1105 42-43 0.3455450842067975 1105 44-45 0.3458703577644826 1105 46-47 0.2927000138546205 1105 48-49 0.38204735808724877 1105 50-51 0.3529554640464525 1105 52-53 0.4894783878330671 1105 54-55 0.5848655712231761 1105 56-57 0.6069183411332872 1105 58-59 0.5698684102285938 1105 60-61 0.574104833817259 1105 62-63 0.6185233145424647 1105 64-65 0.6227251079735368 1105 66-67 0.4648585057768386 1105 68-69 0.5758511837357858 1105 70-71 0.558350455987501 1105 72-73 0.3735265891177555 1105 74-75 0.4082294432554363 1105 76-77 0.6156458104800429 1105 78-79 0.606599757271745 1105 80-81 0.512287826071951 1105 82-83 0.49476547990520814 1105 84-85 0.6580540360290037 1105 86-87 0.704911374741247 1105 88-89 0.8022596815228127 1105 90-91 0.7821956946549662 1105 92-93 0.8836285496130039 1105 94-95 0.7665764337759953 1105 96-97 0.6632410541201104 1105 98-99 0.7095981514088834 1105 100-101 0.5389517185368184 1106 1 0.05945600970105147 1106 2 0.12173342886328697 1106 3 0.19806878047214838 1106 4 0.07584028740508586 1106 5 0.03593943593998006 1106 6 0.11914259060288401 1106 7 0.10797238081981675 1106 8 0.06455278668022402 1106 9 0.12725839224798108 1106 10-11 0.1446762791495857 1106 12-13 0.09664473898518366 1106 14-15 0.09572601096439115 1106 16-17 0.06074520972697428 1106 18-19 0.0986058480513421 1106 20-21 0.22343775474391947 1106 22-23 0.19335701139186767 1106 24-25 0.09258990831302327 1106 26-27 0.14537884429648074 1106 28-29 0.22852068713265083 1106 30-31 0.18002822556727693 1106 32-33 0.09511777567720969 1106 34-35 0.19561826522728154 1106 36-37 0.05938498232060141 1106 38-39 0.10982007837203867 1106 40-41 0.2494897768146842 1106 42-43 0.3436811540744955 1106 44-45 0.31254642322958404 1106 46-47 0.21586261059422185 1106 48-49 0.030830090021147782 1106 50-51 0.11681411601141889 1106 52-53 0.17991436841217734 1106 54-55 0.05597811688773646 1106 56-57 0.15822143506932917 1106 58-59 0.2106700782679809 1106 60-61 0.28850379302399887 1106 62-63 0.28097016804735375 1106 64-65 0.19758134345795497 1106 66-67 0.25594448746896603 1106 68-69 0.19922525344023967 1106 70-71 0.11878101989067602 1106 72-73 0.13815445853381547 1106 74-75 0.2100782858428758 1106 76-77 0.19476514306641945 1106 78-79 0.2325777888170819 1106 80-81 0.21604882946788706 1106 82-83 0.18296627781372976 1106 84-85 0.31231602011535387 1106 86-87 0.39702751420952254 1106 88-89 0.5604941419699472 1106 90-91 0.5446489789166762 1106 92-93 0.4290707507126612 1106 94-95 0.746444702195781 1106 96-97 0.6554759152528611 1106 98-99 0.5537868496010958 1106 100-101 0.6513306780898311 1107 1 0.06738862333588713 1107 2 0.02233570120520767 1107 3 0.07696669385124011 1107 4 0.10143461435765744 1107 5 0.13434018481756738 1107 6 0.11787558687821331 1107 7 0.0034951243489587114 1107 8 0.041747823710082343 1107 9 0.09231981424791513 1107 10-11 0.10708745048530233 1107 12-13 0.12667744323294272 1107 14-15 0.1139648395103805 1107 16-17 0.17040333322711376 1107 18-19 0.19797723565937275 1107 20-21 0.1340461704687712 1107 22-23 0.2186764239298924 1107 24-25 0.24415607664802508 1107 26-27 0.23193571869466467 1107 28-29 0.2280144692247248 1107 30-31 0.11384261969486431 1107 32-33 0.1080710078931375 1107 34-35 0.1844921965428341 1107 36-37 0.15829336862976362 1107 38-39 0.1510791643092091 1107 40-41 0.24179658950302496 1107 42-43 0.2742406593231905 1107 44-45 0.18375012408548486 1107 46-47 0.2574347014973668 1107 48-49 0.12010535221899232 1107 50-51 0.1899446625043666 1107 52-53 0.2719595944358346 1107 54-55 0.16999126009953613 1107 56-57 0.1685661348440206 1107 58-59 0.2155048969750908 1107 60-61 0.2064994887128293 1107 62-63 0.20174954962399028 1107 64-65 0.2153745204386439 1107 66-67 0.2562064684457752 1107 68-69 0.36884531793393904 1107 70-71 0.3054231445705895 1107 72-73 0.4571054936425831 1107 74-75 0.3119304487631851 1107 76-77 0.3329810068741672 1107 78-79 0.27699752303493597 1107 80-81 0.36469109452291804 1107 82-83 0.4857615564780495 1107 84-85 0.5304971213660323 1107 86-87 0.640192941553174 1107 88-89 0.8590530888726633 1107 90-91 0.8710557465745694 1107 92-93 0.8499490257706022 1107 94-95 0.8431822425240583 1107 96-97 0.8589914444473372 1107 98-99 0.8148181650815545 1107 100-101 0.8684564423653107 1108 1 0.11221712847926568 1108 2 0.11714422328060436 1108 3 0.12836916469792925 1108 4 0.12783141864779424 1108 5 0.08224298121572104 1108 6 0.0293234601066672 1108 7 0.1010377314575166 1108 8 0.11155873630464441 1108 9 0.18686154070289973 1108 10-11 0.10007467819605154 1108 12-13 0.13183876033071584 1108 14-15 0.16390715540885736 1108 16-17 0.13323171036940096 1108 18-19 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-0.7926051997359167 2316 78-79 -0.9355658867260814 2316 80-81 -0.7645456402198256 2316 82-83 -0.7755482330532182 2316 84-85 -0.8062590745931892 2316 86-87 -0.9073611111371882 2316 88-89 -1.1738451571683264 2316 90-91 -1.2909642102893244 2316 92-93 -1.1366066144086737 2316 94-95 -0.8591361583465833 2316 96-97 -0.8624668633828172 2316 98-99 -0.6106960584233008 2316 100-101 0.04996217205922093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 23.0 5 79.0 6 156.0 7 395.0 8 813.0 9 1606.0 10 2685.0 11 4338.0 12 6510.0 13 9125.0 14 11178.0 15 15851.0 16 21118.0 17 30000.0 18 28136.0 19 17133.0 20 14536.0 21 15223.0 22 16099.0 23 17589.0 24 20194.0 25 23469.0 26 27779.0 27 34016.0 28 40750.0 29 49996.0 30 60260.0 31 74155.0 32 92590.0 33 118666.0 34 163320.0 35 240435.0 36 373266.0 37 582456.0 38 439397.0 39 28328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71095533062563 19.634323763128197 14.725451462335892 21.929269443910286 2 15.507898351382318 21.48037051870915 39.46289971096318 23.54883141894535 3 19.340638143099177 25.980389498612915 30.342978481437267 24.335993876850637 4 12.554431652591841 17.06273981495756 37.15982395945583 33.223004572994775 5 13.034390810937694 38.705369143634485 33.503481069289045 14.756758976138777 6 30.765455645514272 36.73448044256487 17.37222844986865 15.1278354620522 7 28.077281520003655 34.29637623282222 20.326431104111357 17.29991114306277 8 26.71843661370628 34.36203120606056 19.7103589216006 19.209173258632564 9 27.411125632243838 16.754800559945895 20.622130257917526 35.211943549892744 10-11 25.05147814715855 27.039991044584184 26.671141792276188 21.23738901598108 12-13 26.442433951871614 24.732112215388597 27.49105426780567 21.334399564934124 14-15 23.008462036652556 25.683691192602552 26.87539122297075 24.432455547774133 16-17 21.32293201330385 29.288002542550633 27.462994248466927 21.926071195678592 18-19 21.20797837994451 27.198173900143203 30.74670069106244 20.847147028849854 20-21 22.32068103006669 26.062627884389123 30.509116649930135 21.107574435614055 22-23 21.736870361059747 26.28461024186595 30.44550869628203 21.533010700792275 24-25 21.7696010114352 26.493563488552795 30.75296377007512 20.983871729936887 26-27 21.44998195937635 26.523106039551475 30.87303642385453 21.153875577217647 28-29 21.020533931807144 26.90381991867305 31.048701658378775 21.026944491141034 30-31 21.067673815247293 26.685086211371058 30.80147872817602 21.445761245205638 32-33 21.009436498277477 26.88819054893755 30.49987256350327 21.6025003892817 34-35 21.77586899787441 26.63828674629959 30.317540708174562 21.268303547651442 36-37 21.98360057214964 26.1993982163419 30.482191987774517 21.334809223733945 38-39 21.520599039123397 26.592045013927407 30.58921576184701 21.29814018510219 40-41 21.38845225081868 26.640207561684758 30.692413291942884 21.278926895553678 42-43 21.410334351213933 26.84032418531947 29.94914341348231 21.800198049984285 44-45 21.32376424261193 27.01796199361996 29.757532550460546 21.90074121330756 46-47 21.861781822013022 27.249627373268176 29.04700907009101 21.84158173462779 48-49 21.909935007160097 27.298619613900932 28.86743033285832 21.924015046080655 50-51 21.809813396341525 27.570685698238545 28.798338288126896 21.821162617293037 52-53 22.037671637466655 27.702226111167278 28.56219039019986 21.69791186116621 54-55 21.29471607225998 27.555663409611885 28.762456046028976 22.387164472099155 56-57 21.77610795884261 27.931724298079853 28.239356954010624 22.052810789066918 58-59 21.953260679887972 27.16319406852257 28.54389875490802 22.33964649668144 60-61 21.60721752530978 27.44963948590452 29.110702201160844 21.83244078762486 62-63 21.73122764504141 27.380945925112776 28.935228044787 21.95259838505881 64-65 21.38794720948717 27.444206786599096 28.896054346091454 22.27179165782228 66-67 21.334818164651654 27.69635845119182 28.53635980690737 22.432463577249155 68-69 21.455472985143135 27.541480495887292 28.726097996037396 22.276948522932173 70-71 21.500861067879107 27.69235745123908 28.45988151891909 22.346899961962723 72-73 21.590066615588775 27.51193216195048 28.347667679702894 22.55033354275785 74-75 21.297780222355424 28.049940397280448 28.061154036474345 22.591125343889775 76-77 21.435377469228637 27.53174517950762 28.423559813640704 22.609317537623035 78-79 21.468462504740142 27.59743391204076 28.352235159139056 22.581868424080046 80-81 21.380786799877445 28.06153219249688 28.130983280996467 22.426697726629204 82-83 21.477639736636405 27.779723110318173 28.09934081632179 22.643296336723633 84-85 21.20915156731397 27.636134328138525 28.053885883522266 23.100828221025242 86-87 21.4174856931695 27.391310838564355 28.1360589904594 23.055144477806746 88-89 21.345748702309724 27.294332542695326 28.00197980266904 23.357938952325906 90-91 21.072643574491988 27.34073319237439 28.33022303092027 23.256400202213353 92-93 21.248447715013256 27.338459714960578 28.237313096366005 23.175779473660153 94-95 20.879540360959034 27.009080915590555 28.341915606826255 23.76946311662415 96-97 20.94039225650914 26.773226560399866 28.311160415822577 23.97522076726842 98-99 20.87236808365425 26.99515895500361 27.88849405463277 24.24397890670937 100-101 20.85885138644807 26.38649648734985 28.69912835456443 24.05552377163765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1983.0 1 1331.5 2 944.0 3 1259.0 4 1084.5 5 1021.0 6 1920.0 7 4031.0 8 6157.0 9 6776.0 10 5724.0 11 4660.5 12 4621.0 13 4854.0 14 5156.5 15 5337.5 16 5328.5 17 5433.5 18 5642.5 19 5911.0 20 6434.0 21 7289.5 22 8377.0 23 9828.5 24 11322.0 25 13349.0 26 16831.0 27 20508.5 28 23095.5 29 26103.5 30 30623.0 31 36001.0 32 43518.5 33 49659.5 34 54165.0 35 57798.5 36 62654.0 37 69108.5 38 72060.5 39 72767.0 40 73192.5 41 76563.0 42 80532.0 43 83721.5 44 87469.5 45 91758.0 46 91139.5 47 93088.5 48 113699.5 49 114259.5 50 94421.0 51 85807.0 52 80585.5 53 79784.5 54 79485.5 55 74273.5 56 65919.0 57 58791.5 58 54134.5 59 47626.0 60 39420.5 61 32517.0 62 27301.0 63 21391.5 64 15693.0 65 12223.0 66 8912.5 67 6993.0 68 5250.5 69 3288.5 70 2509.5 71 2158.5 72 1880.0 73 1690.0 74 1390.5 75 1174.5 76 839.0 77 445.0 78 262.0 79 165.5 80 100.0 81 56.5 82 31.5 83 23.5 84 12.0 85 5.5 86 5.0 87 3.5 88 1.5 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29217431453496523 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001162034924572313 16-17 7.165882034862597E-4 18-19 3.873449748574377E-5 20-21 1.9367248742871884E-5 22-23 0.0 24-25 0.0 26-27 1.7430523868584694E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015687471481726227 36-37 0.007185249283605468 38-39 0.0029825563064022702 40-41 0.0032536977888024763 42-43 2.517742336573345E-4 44-45 0.0012588711682866723 46-47 2.324069849144626E-4 48-49 3.486104773716939E-4 50-51 1.162034924572313E-4 52-53 3.873449748574377E-5 54-55 0.0 56-57 0.0010651986808579536 58-59 0.002382171595373242 60-61 6.778537060005159E-4 62-63 0.0013944419094867756 64-65 7.746899497148754E-5 66-67 6.003847110290284E-4 68-69 0.0016655833918869818 70-71 0.0 72-73 0.0 74-75 1.9367248742871884E-5 76-77 0.261825835754885 78-79 1.9367248742871885E-4 80-81 1.9367248742871885E-4 82-83 0.0 84-85 0.0 86-87 0.10661670432950972 88-89 0.3758989308504004 90-91 0.27770697972403996 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.5493798994297507E-4 100-101 3.486104773716939E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2581678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.81155851139442 #Duplication Level Percentage of deduplicated Percentage of total 1 66.11602200318224 24.338338125107843 2 15.592473969639645 11.479665357415685 3 6.462781997492506 7.137152329412457 4 3.4546822156606316 5.086889460802603 5 2.1183350908367076 3.8989608071537742 6 1.368058732599277 3.021622444926143 7 0.9105753213078358 2.346378770354862 8 0.6725463641677375 1.9805983868949 9 0.5022171587522203 1.6638656692350255 >10 2.421070771960503 16.23555117776922 >50 0.21661618980279365 5.548799566354919 >100 0.15062072052333514 10.374650233245896 >500 0.0090522850408154 2.2541760512118394 >1k 0.004526142520407566 2.873826481130981 >5k 2.1051825676314263E-4 0.6048488751274758 >10k+ 2.1051825676314263E-4 1.1546762638564239 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16619 0.6437286137155757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13181 0.5105594113595886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9312 0.360695640587246 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6298 0.24394986516521425 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4560 0.1766293085349916 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3895 0.15087086770697197 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3041 0.1177916068541468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3027 0.11724932388934639 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2708 0.10489301919139413 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2708 0.10489301919139413 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9367248742871884E-5 0.0 14-15 0.0 0.0 0.0 3.873449748574377E-5 0.0 16-17 0.0 0.0 0.0 3.873449748574377E-5 0.0 18-19 0.0 0.0 0.0 7.746899497148754E-5 0.0 20-21 0.0 0.0 0.0 1.7430523868584694E-4 0.0 22-23 0.0 0.0 0.0 4.260794723431815E-4 0.0 24-25 0.0 0.0 0.0 9.102606909149785E-4 0.0 26-27 0.0 0.0 0.0 0.0016074816456583664 0.0 28-29 0.0 0.0 0.0 0.004376998215889046 0.0 30-31 0.0 0.0 0.0 0.012801751419038315 0.0 32-33 0.0 0.0 0.0 0.02833428491082157 0.0 34-35 0.0 0.0 0.0 0.0520785318695825 0.0 36-37 0.0 0.0 0.0 0.09352444417932833 0.0 38-39 0.0 0.0 0.0 0.1572620597921197 0.0 40-41 0.0 0.0 0.0 0.22963746834423193 0.0 42-43 0.0 0.0 0.0 0.3036397257907454 0.0 44-45 0.0 0.0 0.0 0.39245792852555583 0.0 46-47 0.0 0.0 0.0 0.5065116563723284 0.0 48-49 0.0 0.0 0.0 0.6301909068443082 0.0 50-51 0.0 0.0 0.0 0.7682212886347561 0.0 52-53 0.0 0.0 0.0 0.9081109263045197 0.0 54-55 0.0 0.0 0.0 1.056967600142233 0.0 56-57 0.0 0.0 0.0 1.2364632614911697 0.0 58-59 0.0 0.0 0.0 1.4426857261052695 0.0 60-61 0.0 0.0 0.0 1.679043629763278 0.0 62-63 0.0 0.0 0.0 1.9148979849539718 0.0 64-65 0.0 0.0 0.0 2.1552649091017546 0.0 66-67 0.0 0.0 0.0 2.429582620295792 0.0 68-69 0.0 0.0 0.0 2.743603191412717 0.0 70-71 0.0 0.0 0.0 3.0990503075906446 0.0 72-73 0.0 0.0 0.0 3.4633288891953216 0.0 74-75 0.0 0.0 0.0 3.826251763387998 0.0 76-77 0.0 0.0 0.0 4.2238807473279 0.0 78-79 0.0 3.873449748574377E-5 0.0 4.687125970008653 0.0 80-81 0.0 3.873449748574377E-5 0.0 5.198595642059157 0.0 82-83 0.0 3.873449748574377E-5 0.0 5.721821234096584 0.0 84-85 0.0 3.873449748574377E-5 0.0 6.264007362653282 0.0 86-87 1.9367248742871884E-5 3.873449748574377E-5 0.0 6.8306349591234845 0.0 88-89 3.873449748574377E-5 3.873449748574377E-5 0.0 7.438204919436119 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 160 6.134542E-8 26.769312 1 GTACATG 16585 0.0 26.083334 1 TACATGG 16775 0.0 25.173576 2 ACATGGG 16975 0.0 23.926626 3 GAGTACT 13630 0.0 22.941607 12-13 GTATCAA 26635 0.0 22.459356 1 GGTATCA 20345 0.0 22.455786 1 GTACTTT 14520 0.0 21.404692 14-15 AGTACTT 14105 0.0 21.361654 12-13 CATGGGA 11050 0.0 20.997328 4 CATGGGG 5615 0.0 20.872026 4 AGTACAT 13030 0.0 19.153969 2 ATGGGAG 3960 0.0 19.051071 5 ATCAACG 31195 0.0 19.012642 3 ACTTTTT 16585 0.0 18.968288 16-17 ATGGGAT 4605 0.0 18.958572 5 TCAACGC 31350 0.0 18.91864 4 CAACGCA 31520 0.0 18.846712 5 TATCAAC 31600 0.0 18.844044 2 AACGCAG 32035 0.0 18.558538 6 >>END_MODULE