FastQCFastQC Report
Fri 27 May 2016
SRR939336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631394
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167610.6369627657431765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125930.4785676337332988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88130.33491753800457097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60420.22961213714099826No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA46080.1751163071740682No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38760.14729835212818757No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32890.12499078435232427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29420.11180385757511037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.10899165993386016No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26850.10203717117239No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26520.1007830830350757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG172900.028.0482351
TACATGG175650.027.079532
ACATGGG178450.025.8308123
CATGGGG58500.023.4277364
GAGTACT126350.023.011612-13
GTATCAA269750.022.644061
CATGGGA117200.022.1333964
GGTATCA206850.021.6536431
AGTACTT128950.021.55530512-13
GTACTTT135000.021.37917714-15
ATGGGAG43350.020.5659525
CCGTCGT1404.930179E-420.3101989
AGTACAT129150.019.3143312
ATCAACG315100.019.2039533
TCAACGC316550.019.1309684
CAACGCA316200.019.1217845
TATCAAC318500.019.1180652
GTAAGGT9450.019.0695364
ATGGGGA33150.018.8829785
AACGCAG320450.018.870776
Produced by FastQC (version 0.11.5)