##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2631394 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00376606467903 33.0 31.0 34.0 30.0 34.0 2 32.06480595456249 33.0 31.0 34.0 30.0 34.0 3 32.10963200493731 34.0 31.0 34.0 30.0 34.0 4 35.62040880233063 37.0 35.0 37.0 33.0 37.0 5 35.66872007764706 37.0 35.0 37.0 33.0 37.0 6 35.77562539095248 37.0 35.0 37.0 35.0 37.0 7 35.70594635390975 37.0 35.0 37.0 33.0 37.0 8 35.688326415580484 37.0 35.0 37.0 33.0 37.0 9 37.32928326202766 39.0 37.0 39.0 34.0 39.0 10-11 37.24661187188235 39.0 37.0 39.0 34.0 39.0 12-13 37.154585174246044 39.0 37.0 39.0 33.5 39.0 14-15 38.391763643148835 40.0 38.0 41.0 34.0 41.0 16-17 38.345706876279266 40.0 38.0 41.0 33.0 41.0 18-19 38.33635422897521 40.0 38.0 41.0 33.5 41.0 20-21 38.25878583746866 40.0 38.0 41.0 34.0 41.0 22-23 38.18447218470514 40.0 38.0 41.0 33.5 41.0 24-25 38.19121613867023 40.0 38.0 41.0 33.0 41.0 26-27 38.104098436038086 40.0 38.0 41.0 33.0 41.0 28-29 37.968167632821235 40.0 38.0 41.0 33.0 41.0 30-31 37.767369690741866 40.0 37.5 41.0 33.0 41.0 32-33 37.5401397510217 40.0 37.0 41.0 32.5 41.0 34-35 37.22524221002252 40.0 36.0 41.0 31.5 41.0 36-37 36.96987281266128 40.0 36.0 41.0 31.0 41.0 38-39 36.64631940332767 39.5 35.5 41.0 30.5 41.0 40-41 36.50676675556758 39.0 35.0 41.0 30.0 41.0 42-43 36.30360884762981 39.0 35.0 41.0 30.0 41.0 44-45 36.183691799859695 39.0 35.0 41.0 30.0 41.0 46-47 35.90925133218363 39.0 35.0 41.0 28.5 41.0 48-49 35.64616454244404 39.0 35.0 41.0 27.0 41.0 50-51 34.467633315269396 38.0 34.0 40.0 24.5 40.5 52-53 34.57138022660233 38.5 34.5 39.5 25.0 40.5 54-55 35.06369798669451 39.0 35.0 40.5 26.0 41.0 56-57 34.964869381020094 39.0 35.0 41.0 25.5 41.0 58-59 34.77496414448007 38.5 35.0 41.0 25.0 41.0 60-61 34.48435259030005 38.0 34.0 40.0 24.0 41.0 62-63 34.14103247176212 37.5 34.0 40.0 23.0 41.0 64-65 33.76913168457479 37.0 34.0 40.0 22.0 41.0 66-67 33.4107288760254 36.5 33.5 39.5 21.5 41.0 68-69 33.00560102363994 36.0 33.0 39.0 20.0 41.0 70-71 32.587491458899734 35.5 33.0 39.0 18.5 40.5 72-73 32.1628083821731 35.0 32.0 38.0 17.5 40.0 74-75 31.72676744721619 35.0 32.0 37.0 13.5 39.5 76-77 31.299397771675395 35.0 31.5 37.0 9.5 39.0 78-79 30.878794661688822 35.0 31.0 36.0 7.0 39.0 80-81 30.429757003322194 35.0 31.0 36.0 2.0 37.5 82-83 30.007877953662586 34.5 30.5 35.0 2.0 37.0 84-85 29.60732600287148 34.0 30.0 35.0 2.0 36.5 86-87 29.29942475357168 34.0 30.0 35.0 2.0 36.0 88-89 28.898681269319606 34.0 29.0 35.0 2.0 36.0 90-91 28.519219850771112 34.0 29.0 35.0 2.0 35.5 92-93 28.132396554829874 34.0 29.0 35.0 2.0 35.0 94-95 27.689871794189695 34.0 28.0 35.0 2.0 35.0 96-97 27.161854135108612 34.0 26.5 35.0 2.0 35.0 98-99 26.479525681064864 33.5 25.0 35.0 2.0 35.0 100-101 24.093983835183934 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2902888403640631 1101 2 0.1389008860188028 1101 3 0.35753044379512744 1101 4 0.27279014970623194 1101 5 0.3453018302358828 1101 6 0.41823206383622136 1101 7 0.3356042477726078 1101 8 0.2451007563963188 1101 9 0.3585689598369797 1101 10-11 0.43488775605747065 1101 12-13 0.3996604727714441 1101 14-15 0.5070866795878644 1101 16-17 0.5414787267019392 1101 18-19 0.563565688695995 1101 20-21 0.5787953806867918 1101 22-23 0.5871477092341522 1101 24-25 0.5814511128259383 1101 26-27 0.5352125190004386 1101 28-29 0.5089525200883216 1101 30-31 0.5813618119745172 1101 32-33 0.5835988519407138 1101 34-35 0.5895690651758443 1101 36-37 0.6013335329856986 1101 38-39 0.7034882819232209 1101 40-41 0.49262992581895304 1101 42-43 0.3845213093872246 1101 44-45 0.39451436633561343 1101 46-47 0.41295397985749815 1101 48-49 0.3711802774834183 1101 50-51 0.3575844497638556 1101 52-53 0.4636553722880805 1101 54-55 0.4575169318509822 1101 56-57 0.41330366557857445 1101 58-59 0.2900289303465229 1101 60-61 0.38952254924563334 1101 62-63 0.373732265143893 1101 64-65 0.4908825955574869 1101 66-67 0.30949745745635227 1101 68-69 0.20471634163904184 1101 70-71 0.31639444793900395 1101 72-73 0.4476999777305437 1101 74-75 0.45258612379712915 1101 76-77 0.4482344606840414 1101 78-79 0.1566812496685337 1101 80-81 0.18144453941894412 1101 82-83 0.28935795022232824 1101 84-85 0.21350217308539854 1101 86-87 0.30473965309358064 1101 88-89 0.22243498026726272 1101 90-91 0.1853086264159991 1101 92-93 0.23905140161447846 1101 94-95 0.23191539126504424 1101 96-97 0.19363707145343056 1101 98-99 -0.03854125333842262 1101 100-101 -0.09529176073711909 1102 1 0.2914522286829424 1102 2 0.34827544906322316 1102 3 0.3647046402692453 1102 4 0.29021017502603286 1102 5 0.29270258227185764 1102 6 0.3455604465809614 1102 7 0.28299063932259827 1102 8 0.355504361634118 1102 9 0.3430452744827903 1102 10-11 0.4191456401532392 1102 12-13 0.38832107402232197 1102 14-15 0.44518372624055047 1102 16-17 0.3935290639844098 1102 18-19 0.4771071545992953 1102 20-21 0.4097899388184132 1102 22-23 0.34689932951266655 1102 24-25 0.45549746318400963 1102 26-27 0.4921072528590642 1102 28-29 0.3861705538354627 1102 30-31 0.3858351033601153 1102 32-33 0.4479269323594295 1102 34-35 0.5416018297583491 1102 36-37 0.4487387254351063 1102 38-39 0.4925370886802085 1102 40-41 0.5065157599553416 1102 42-43 0.34052342566937455 1102 44-45 0.4203141131375716 1102 46-47 0.5214371535249 1102 48-49 0.5936601066692191 1102 50-51 0.5440778458298325 1102 52-53 0.6500563699639557 1102 54-55 0.5628732986480216 1102 56-57 0.6267177766455916 1102 58-59 0.6485175836730548 1102 60-61 0.622927307101385 1102 62-63 0.6246752388982202 1102 64-65 0.6020036582334498 1102 66-67 0.5799117385817567 1102 68-69 0.6226813096113801 1102 70-71 0.5076378581765226 1102 72-73 0.6019154417065025 1102 74-75 0.4624892849935769 1102 76-77 0.4928717639359341 1102 78-79 0.3133196677476313 1102 80-81 0.21614873230294407 1102 82-83 0.18229873211299719 1102 84-85 0.3248171023222177 1102 86-87 0.23557586797180008 1102 88-89 0.2827700897659646 1102 90-91 0.2475620889937069 1102 92-93 0.29123684083750234 1102 94-95 0.2982023553449338 1102 96-97 0.2232294981866083 1102 98-99 0.2085252394907826 1102 100-101 0.15391264944370775 1103 1 0.2932950621888821 1103 2 0.33479187368691754 1103 3 0.25053219484762224 1103 4 0.3134235091190689 1103 5 0.32899953151377304 1103 6 0.30152627791169806 1103 7 0.27811793951286035 1103 8 0.3369240863765768 1103 9 0.4821850695199288 1103 10-11 0.36180776648927804 1103 12-13 0.3098461961051484 1103 14-15 0.5246892279280928 1103 16-17 0.43835365684612526 1103 18-19 0.5642096661931078 1103 20-21 0.5246086886857526 1103 22-23 0.5504760129486215 1103 24-25 0.4812549576901475 1103 26-27 0.48131475066095675 1103 28-29 0.4754373753470631 1103 30-31 0.4852450503039307 1103 32-33 0.46185208393654165 1103 34-35 0.42818573370532675 1103 36-37 0.4680421131429213 1103 38-39 0.6544070256361962 1103 40-41 0.5979616943819721 1103 42-43 0.4318090408428361 1103 44-45 0.38740956398100224 1103 46-47 0.30908620294903244 1103 48-49 0.299409479077994 1103 50-51 0.15926583272275963 1103 52-53 0.1337049233480272 1103 54-55 0.20813818292210584 1103 56-57 0.32750227963914824 1103 58-59 0.21569358516564563 1103 60-61 0.32319342588957767 1103 62-63 0.23738805293123733 1103 64-65 0.16223872982968146 1103 66-67 0.005980263611114367 1103 68-69 0.022227611710945894 1103 70-71 -0.08603187745870855 1103 72-73 -0.02449684802110852 1103 74-75 0.0034704053067571294 1103 76-77 0.03698972315563154 1103 78-79 0.009916039728516068 1103 80-81 -0.09724105351723367 1103 82-83 -0.09546247375591577 1103 84-85 -0.16091420484388763 1103 86-87 -0.13675973217522142 1103 88-89 -0.13735209920071512 1103 90-91 -0.0823274527166653 1103 92-93 0.03154311205745941 1103 94-95 -0.06847263451640728 1103 96-97 -0.16084711318946532 1103 98-99 -0.22213165608049934 1103 100-101 -0.404093034907234 1104 1 0.16389106440927037 1104 2 0.2359759019699439 1104 3 0.3420860905000751 1104 4 0.2803774252508475 1104 5 0.2272205249765591 1104 6 0.28218247856596435 1104 7 0.3043956068100755 1104 8 0.30308362031027514 1104 9 0.48385197870490515 1104 10-11 0.4282119744514219 1104 12-13 0.40120499925034636 1104 14-15 0.42289836494015276 1104 16-17 0.5294305998219855 1104 18-19 0.4495227573314935 1104 20-21 0.4511520482649516 1104 22-23 0.5181836220953357 1104 24-25 0.5939691148312249 1104 26-27 0.5636219620776473 1104 28-29 0.5104993253020709 1104 30-31 0.4809042420173597 1104 32-33 0.5232666124001071 1104 34-35 0.5767087971966234 1104 36-37 0.5490251745862835 1104 38-39 0.7138073048017191 1104 40-41 0.5690915063494444 1104 42-43 0.5609045014389977 1104 44-45 0.6022894096315738 1104 46-47 0.6828414103560831 1104 48-49 0.5744160809653351 1104 50-51 0.5897902650951821 1104 52-53 0.6717220936443908 1104 54-55 0.7355567641360921 1104 56-57 0.7334709247163005 1104 58-59 0.7944328133106993 1104 60-61 0.8138284466114172 1104 62-63 0.6461354189129267 1104 64-65 0.8127329282527782 1104 66-67 0.7899910314240799 1104 68-69 0.7590887780623348 1104 70-71 0.6643367503726978 1104 72-73 0.4735832153570101 1104 74-75 0.5460493450167085 1104 76-77 0.5022751133137007 1104 78-79 0.5221424656014477 1104 80-81 0.512941212466071 1104 82-83 0.5557406865271304 1104 84-85 0.42965749165253087 1104 86-87 0.3106520585260846 1104 88-89 0.3665198403529395 1104 90-91 0.3240270659383775 1104 92-93 0.41544097412382186 1104 94-95 0.39071449800098534 1104 96-97 0.4271107748533005 1104 98-99 0.46271660829679817 1104 100-101 0.3288778682848488 1105 1 0.2949661013229061 1105 2 0.19519139579471556 1105 3 0.2768555040576004 1105 4 0.38419448860454253 1105 5 0.32387785023409776 1105 6 0.30104609829742657 1105 7 0.3308287812964039 1105 8 0.27352946811848966 1105 9 0.3794313828349303 1105 10-11 0.39153984069277925 1105 12-13 0.27567109682286883 1105 14-15 0.3242431834714452 1105 16-17 0.3099208300855665 1105 18-19 0.44978879377589465 1105 20-21 0.5069245655809951 1105 22-23 0.518747120001052 1105 24-25 0.3772983586075682 1105 26-27 0.34609369032442316 1105 28-29 0.3254305131246511 1105 30-31 0.4668077543010938 1105 32-33 0.4016905401828339 1105 34-35 0.3083361395442026 1105 36-37 0.4116875192369278 1105 38-39 0.5598234305706669 1105 40-41 0.5675482366840541 1105 42-43 0.42239292799270345 1105 44-45 0.5794442833073177 1105 46-47 0.3996770402985277 1105 48-49 0.4738586097751778 1105 50-51 0.37478202140923855 1105 52-53 0.3676282318541908 1105 54-55 0.39155183944851757 1105 56-57 0.42763318972819775 1105 58-59 0.5286830683699151 1105 60-61 0.6008929626015842 1105 62-63 0.4766770914986864 1105 64-65 0.4165874433389334 1105 66-67 0.4752634604917958 1105 68-69 0.4942637034817352 1105 70-71 0.39622883772563 1105 72-73 0.5397823810873845 1105 74-75 0.4479735892020038 1105 76-77 0.49337150201963453 1105 78-79 0.37387837492520504 1105 80-81 0.4419525297769482 1105 82-83 0.35800673158113483 1105 84-85 0.4253350451503941 1105 86-87 0.296447089929579 1105 88-89 0.2678956534214443 1105 90-91 0.23709685960946913 1105 92-93 0.3579934603592321 1105 94-95 0.2093491025408234 1105 96-97 0.1155851118069684 1105 98-99 0.07829652900244 1105 100-101 -0.2445270968678237 1106 1 0.2196492082570387 1106 2 0.2226710922618338 1106 3 0.1770552487003627 1106 4 0.20843187211425374 1106 5 0.2579418804031022 1106 6 0.22848499025762692 1106 7 0.23643479403794032 1106 8 0.24693743421126158 1106 9 0.2347228059908275 1106 10-11 0.30122136809760747 1106 12-13 0.36739946385282707 1106 14-15 0.4221570530549741 1106 16-17 0.46186891622256354 1106 18-19 0.5261245655075939 1106 20-21 0.4262399877102041 1106 22-23 0.48175577779602463 1106 24-25 0.4816545687902689 1106 26-27 0.45155762769609 1106 28-29 0.4169350312365907 1106 30-31 0.400567149209742 1106 32-33 0.31957920311128163 1106 34-35 0.3299411387544424 1106 36-37 0.26794097445726806 1106 38-39 0.40635512974930776 1106 40-41 0.32654645313222375 1106 42-43 0.1554069002456373 1106 44-45 0.1693872063579107 1106 46-47 0.1741628706044338 1106 48-49 0.22094511862054844 1106 50-51 0.3252429642566099 1106 52-53 0.22271160138507895 1106 54-55 0.22065795749200134 1106 56-57 0.36999855130878245 1106 58-59 0.45727600392511647 1106 60-61 0.3967790291787523 1106 62-63 0.3435950020446725 1106 64-65 0.42003114628212757 1106 66-67 0.30098749368823974 1106 68-69 0.2295675818842966 1106 70-71 0.3458467890761341 1106 72-73 0.28185758642619163 1106 74-75 0.2934459342765017 1106 76-77 0.3271387945747861 1106 78-79 0.47791522402750886 1106 80-81 0.3849510193520551 1106 82-83 0.5190137473237755 1106 84-85 0.43709505212664723 1106 86-87 0.5500960494146518 1106 88-89 0.5032414880710512 1106 90-91 0.5503176843870179 1106 92-93 0.5342935281473657 1106 94-95 0.47496653396292743 1106 96-97 0.5177821383430619 1106 98-99 0.5487984902513219 1106 100-101 0.43783811663188743 1107 1 0.05645284916013793 1107 2 0.1479225818614438 1107 3 0.04576274341592779 1107 4 0.15236558301705116 1107 5 0.1778333004625381 1107 6 0.14707731862817752 1107 7 0.15261513898172296 1107 8 0.10259370048580507 1107 9 0.21642638715677265 1107 10-11 0.25190470755783423 1107 12-13 0.2718616571255055 1107 14-15 0.2786658183339057 1107 16-17 0.27238725093445026 1107 18-19 0.38233536299863147 1107 20-21 0.3496033906602989 1107 22-23 0.3698115535366071 1107 24-25 0.30292367702173806 1107 26-27 0.3846426784726731 1107 28-29 0.33850440483889344 1107 30-31 0.3177744658626267 1107 32-33 0.230468884666287 1107 34-35 0.33304957701072624 1107 36-37 0.23277215955055652 1107 38-39 0.39439836451633425 1107 40-41 0.17392232621374148 1107 42-43 0.28477757313109464 1107 44-45 0.34097429049540295 1107 46-47 0.04567963913061135 1107 48-49 -0.09082515946357717 1107 50-51 -0.21286747481149604 1107 52-53 -0.1684693658207621 1107 54-55 -0.08482675592281197 1107 56-57 -0.22950856928368069 1107 58-59 -0.3112516091925457 1107 60-61 -0.3004107875507245 1107 62-63 -0.18876310306207955 1107 64-65 -0.20007787402850852 1107 66-67 -0.4286116451010926 1107 68-69 -0.32133321422818995 1107 70-71 -0.38620238685744823 1107 72-73 -0.40880972739300603 1107 74-75 -0.1883562514907382 1107 76-77 -0.24515393472399083 1107 78-79 -0.1716042939395912 1107 80-81 -0.2069396137852202 1107 82-83 -0.25939873774193245 1107 84-85 -0.2645453742281596 1107 86-87 -0.1929817639756628 1107 88-89 -0.2196759923229088 1107 90-91 -0.12861376722588602 1107 92-93 -0.1213210465700314 1107 94-95 -0.1563561270976379 1107 96-97 -0.0608636471239592 1107 98-99 0.12832453718328196 1107 100-101 0.20637386702247795 1108 1 0.054052245468390936 1108 2 0.11856586232605792 1108 3 0.11736417893520468 1108 4 0.16927032262168495 1108 5 0.18020664360357586 1108 6 0.14424420117099146 1108 7 0.07780975113947619 1108 8 0.15110042386660183 1108 9 0.09898960993258044 1108 10-11 0.12872265505156832 1108 12-13 0.10177660877609895 1108 14-15 0.18234896240439014 1108 16-17 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-1.2666554351560464 2316 50-51 -1.1983796776205438 2316 52-53 -1.111434444823935 2316 54-55 -1.0448294978340869 2316 56-57 -1.2138420482810517 2316 58-59 -0.8322570337808557 2316 60-61 -0.7977666686337344 2316 62-63 -0.943154911006097 2316 64-65 -1.094887391291934 2316 66-67 -1.0339707032247674 2316 68-69 -0.9676210724415739 2316 70-71 -0.9702173794494477 2316 72-73 -1.1626047225853426 2316 74-75 -1.1139973688344824 2316 76-77 -0.7498122035423123 2316 78-79 -0.85361653642439 2316 80-81 -0.714441497917857 2316 82-83 -0.439933477496929 2316 84-85 -0.6640979136813208 2316 86-87 -0.5472415429314168 2316 88-89 -0.5239648109503214 2316 90-91 -0.6167143938792741 2316 92-93 -0.7277783593703226 2316 94-95 -0.6850294034096471 2316 96-97 -0.6214231907447534 2316 98-99 -0.56878758947812 2316 100-101 -0.2581079508646944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7032.0 3 1759.0 4 1667.0 5 1948.0 6 2139.0 7 2737.0 8 3312.0 9 4227.0 10 5380.0 11 7133.0 12 10048.0 13 15484.0 14 14414.0 15 16530.0 16 21809.0 17 31254.0 18 24628.0 19 15847.0 20 15126.0 21 15812.0 22 16457.0 23 18610.0 24 20861.0 25 23250.0 26 27132.0 27 32145.0 28 37757.0 29 45034.0 30 54704.0 31 66797.0 32 83959.0 33 109083.0 34 150397.0 35 222822.0 36 343324.0 37 536925.0 38 532164.0 39 91666.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.679106937677325 18.984376935257362 14.200811355161289 22.135704771904017 2 15.232837728842963 20.95459664287562 40.27251906073565 23.54004656754577 3 19.31403010925506 25.94273107974318 30.05956253838234 24.68367627261942 4 12.32293453429138 16.5503225875187 37.57098219109905 33.55576068709087 5 13.14821234435046 38.53320035206297 33.483806130964226 14.834781172622346 6 30.68147681702688 36.35312405649364 17.803399322690773 15.161999803788703 7 28.24880457012821 34.144648725774815 20.48628725759603 17.12025944650095 8 26.67873999757367 33.99967370061157 20.005689322670165 19.3158969791446 9 27.659990363366493 16.769605738474542 20.66034897999619 34.91005491816278 10-11 25.060544680614804 26.862634258791836 26.84150993119097 21.235311129402383 12-13 26.461140207700463 24.666046283098744 27.532632885551784 21.34018062364901 14-15 23.111782764559567 25.508781923767422 26.922271028645117 24.4571642830279 16-17 21.420459244935387 29.206346490064718 27.44295089541639 21.930243369583508 18-19 21.34233774965778 27.219483978947522 30.574900519086246 20.86327775230845 20-21 22.4642293127103 26.0799662283258 30.28598485028557 21.16981960867833 22-23 21.865287595107045 26.309001359154287 30.260551797242403 21.56515924849627 24-25 21.814286447863157 26.52336729434632 30.564188977156668 21.098157280633856 26-27 21.513338047450286 26.540396039076708 30.669616349744366 21.276649563728636 28-29 21.06170697795689 26.913308459580087 30.88087432275948 21.14411023970354 30-31 21.167567559344054 26.714523262200085 30.599309155027072 21.51860002342879 32-33 21.08694362424071 26.89172321889019 30.323058855386037 21.69827430148306 34-35 21.90128515353577 26.726344123706312 30.032273597210878 21.340097125547036 36-37 22.074934406077016 26.26445898315402 30.20085719309501 21.459749417673954 38-39 21.617450462865165 26.647806090038124 30.327105618974915 21.407637828121796 40-41 21.469166388522318 26.692154918808853 30.492937865761405 21.34574082690742 42-43 21.459030011857397 26.916197617195 29.73089484171737 21.89387752923023 44-45 21.394674857706864 27.047613853577275 29.584615408084964 21.973095880630893 46-47 21.89282118174745 27.274017117488214 28.905747021221412 21.927414679542924 48-49 21.893029780278777 27.339381126346616 28.759039028386102 22.008550064988505 50-51 21.790551718995328 27.587608945606963 28.80859309815837 21.81324623723934 52-53 22.05813416391947 27.72289104697869 28.504788571659606 21.714186217442233 54-55 21.29348496170995 27.51490996056109 28.80320072428934 22.38840435343962 56-57 21.79096300134456 27.940229172469184 28.274187223540476 21.994620602645774 58-59 21.977013211932636 27.131695028780182 28.620205637553802 22.271086121733376 60-61 21.58937338800993 27.445361669745928 29.184258003006157 21.78100693923799 62-63 21.75843154332511 27.307319117472378 29.076691022859023 21.857558316343486 64-65 21.45529148703896 27.43192583534036 28.967022756106324 22.145759921514358 66-67 21.32129674980965 27.73718409404053 28.601953532089162 22.339565624060658 68-69 21.462849360221576 27.471997772029816 28.846224277244946 22.21892859050366 70-71 21.506413380591596 27.664910663991808 28.61031752829191 22.21835842712468 72-73 21.603257086207048 27.42226428293791 28.541959187628045 22.432519443226997 74-75 21.247873612424225 28.035625085947157 28.346894468045015 22.369606833583603 76-77 21.54124807657212 27.516408134185806 28.518106681429234 22.424237107812843 78-79 21.5235439529956 27.597449517895278 28.49918190716486 22.37982462194426 80-81 21.43632412303789 28.048069265865184 28.343635805894973 22.17197080520196 82-83 21.547695387304252 27.69085677206828 28.35247464112789 22.40897319949958 84-85 21.27565850708836 27.59184221420929 28.31024132054391 22.822257958158442 86-87 21.546854713367914 27.343660469482423 28.413436044093753 22.69604877305591 88-89 21.492554505762673 27.231026013936294 28.301898666825338 22.974520813475692 90-91 21.221283794094848 27.26204501199465 28.705733474542644 22.810937719367853 92-93 21.41880940850006 27.23292414233262 28.63081271753194 22.717453731635377 94-95 21.08744337523917 26.932184952314937 28.779484025851925 23.20088764659397 96-97 21.254678683059687 26.617514822426546 28.77919896902064 23.348607525493122 98-99 21.18573367502405 26.842837273991655 28.316645520802417 23.654783530181874 100-101 21.18451889467532 26.317859982799618 29.11313159435324 23.38448952817182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3846.0 1 2343.5 2 1112.5 3 1439.5 4 1250.0 5 1210.0 6 2215.5 7 4398.5 8 6663.0 9 7420.0 10 6374.5 11 5197.5 12 5147.5 13 5486.5 14 5622.5 15 5748.0 16 5947.0 17 6023.5 18 6089.5 19 6304.5 20 6711.0 21 7489.0 22 8738.5 23 10225.0 24 11607.5 25 13780.0 26 17114.0 27 20882.0 28 23734.5 29 26472.5 30 30858.0 31 36466.5 32 44362.5 33 50416.0 34 54771.0 35 58655.0 36 63544.5 37 70380.0 38 73780.5 39 74324.5 40 74531.0 41 77964.5 42 81936.5 43 85341.0 44 89334.5 45 93562.5 46 93089.5 47 94591.0 48 115551.5 49 116517.5 50 95506.5 51 85565.0 52 80170.0 53 79940.0 54 79842.5 55 74742.5 56 66438.0 57 58922.0 58 54023.5 59 47897.5 60 39840.0 61 33133.5 62 28490.5 63 22446.0 64 16271.5 65 12772.0 66 9539.5 67 7617.5 68 5716.0 69 3621.0 70 2832.5 71 2499.0 72 2136.0 73 1852.5 74 1458.5 75 1152.0 76 839.5 77 478.5 78 278.5 79 191.0 80 129.5 81 100.5 82 85.5 83 62.5 84 41.0 85 26.5 86 16.0 87 13.5 88 12.5 89 9.5 90 7.5 91 5.5 92 5.5 93 5.5 94 2.0 95 2.5 96 2.0 97 0.0 98 0.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28080173474591796 2 0.11225988962504285 3 0.12282463211514506 4 0.12426873360659788 5 0.13129922770972344 6 0.05989220922446429 7 0.06825279680655956 8 0.07247109326843491 9 0.07011492767711715 10-11 0.06553560584237861 12-13 0.0698489089813232 14-15 0.0657826232027587 16-17 0.06656167795472666 18-19 0.07104599311239594 20-21 0.07492226553682192 22-23 0.08305483709395096 24-25 0.09116840731566614 26-27 0.09162443936559861 28-29 0.08552501069775184 30-31 0.08170574227956741 32-33 0.07471325084726954 34-35 0.24086092770599915 36-37 0.364103589200249 38-39 0.3350315460170541 40-41 0.3175313161008956 42-43 0.34128298536821167 44-45 0.3550209508724273 46-47 0.3615574102547927 48-49 0.37153311134706546 50-51 0.36541468134380484 52-53 0.3660037227416343 54-55 0.35104967177093205 56-57 0.32718399449113283 58-59 0.312951994266157 60-61 0.2703700016037127 62-63 0.3418150227597996 64-65 0.37121008864502997 66-67 0.37569440380269925 68-69 0.38663917300107853 70-71 0.38741822775304646 72-73 0.3719321393907564 74-75 0.3751053624048698 76-77 0.35903023264475026 78-79 0.33560158607946966 80-81 0.33455651263170777 82-83 0.3490735328878914 84-85 0.3565980617117771 86-87 0.34994759431692857 88-89 0.36509165864176935 90-91 0.38861531188411924 92-93 0.38319993129117114 94-95 0.383085923278688 96-97 0.38751323443011576 98-99 0.37698649461084127 100-101 0.35566699627649834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2631394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.04578284747011 #Duplication Level Percentage of deduplicated Percentage of total 1 67.88847834218306 25.82870304851874 2 14.680110970991489 11.170326283582114 3 6.304162755442118 7.195404216859788 4 3.2886772756137 5.004812059336337 5 1.9447306778324343 3.699440053281307 6 1.2454555613333311 2.843059909959714 7 0.8789511743933631 2.34082698601491 8 0.6131421313038773 1.8661977905777851 9 0.4657721520767305 1.5948599538879098 >10 2.3286337656426546 16.109954541087045 >50 0.20726456900641294 5.5062705252952995 >100 0.14095750017373318 9.99425038422311 >500 0.009041773240000004 2.3156259174057765 >1k 0.004219494178666412 2.8404955194031696 >5k 2.009282942222101E-4 0.5677933249106383 >10k+ 2.009282942222101E-4 1.1219794856564709 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16761 0.6369627657431765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12593 0.4785676337332988 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8813 0.33491753800457097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6042 0.22961213714099826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4608 0.1751163071740682 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3876 0.14729835212818757 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3289 0.12499078435232427 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2942 0.11180385757511037 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2868 0.10899165993386016 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2685 0.10203717117239 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2652 0.1007830830350757 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.800267082770577E-5 0.0 16-17 0.0 0.0 0.0 3.800267082770577E-5 0.0 18-19 0.0 0.0 0.0 5.700400624155865E-5 0.0 20-21 0.0 0.0 0.0 1.330093478969702E-4 0.0 22-23 0.0 0.0 0.0 3.800267082770577E-4 0.0 24-25 0.0 0.0 0.0 8.550600936233798E-4 0.0 26-27 0.0 0.0 0.0 0.001482104162280525 0.0 28-29 0.0 0.0 0.0 0.003971279101495253 0.0 30-31 0.0 0.0 0.0 0.012407872025245934 0.0 32-33 0.0 0.0 0.0 0.02751393367925898 0.0 34-35 0.0 0.0 0.0 0.049460476082259064 0.0 36-37 0.0 0.0 0.0 0.08750114958079253 0.0 38-39 0.0 0.0 0.0 0.14678531607201353 0.0 40-41 0.0 0.0 0.0 0.21477209418277918 0.0 42-43 0.0 0.0 0.0 0.28479201518282704 0.0 44-45 0.0 0.0 0.0 0.367846852276778 0.0 46-47 0.0 0.0 0.0 0.4751283920233914 0.0 48-49 0.0 0.0 0.0 0.595558855876391 0.0 50-51 0.0 0.0 0.0 0.7286062064441889 0.0 52-53 0.0 0.0 0.0 0.8619005743723669 0.0 54-55 0.0 0.0 0.0 1.0052276473990591 0.0 56-57 0.0 0.0 0.0 1.1822630894499266 0.0 58-59 0.0 0.0 0.0 1.3814160859225186 0.0 60-61 3.800267082770577E-5 0.0 0.0 1.6087670641492684 0.0 62-63 3.800267082770577E-5 0.0 0.0 1.8369350997988136 0.0 64-65 3.800267082770577E-5 0.0 0.0 2.0689794078727854 0.0 66-67 3.800267082770577E-5 0.0 0.0 2.344878798081929 0.0 68-69 3.800267082770577E-5 0.0 0.0 2.6558546534650453 0.0 70-71 3.800267082770577E-5 0.0 0.0 3.0105335803000237 0.0 72-73 3.800267082770577E-5 0.0 0.0 3.3662005765765217 0.0 74-75 3.800267082770577E-5 0.0 0.0 3.7322423019889834 0.0 76-77 3.800267082770577E-5 0.0 0.0 4.127242062572158 0.0 78-79 3.800267082770577E-5 0.0 0.0 4.585630278095945 0.0 80-81 3.800267082770577E-5 0.0 0.0 5.092262884235504 0.0 82-83 3.800267082770577E-5 0.0 0.0 5.605013920378324 0.0 84-85 3.800267082770577E-5 0.0 0.0 6.142903723273672 0.0 86-87 3.800267082770577E-5 0.0 0.0 6.713152800378811 0.0 88-89 3.800267082770577E-5 0.0 0.0 7.316806225141503 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17290 0.0 28.048235 1 TACATGG 17565 0.0 27.07953 2 ACATGGG 17845 0.0 25.830812 3 CATGGGG 5850 0.0 23.427736 4 GAGTACT 12635 0.0 23.0116 12-13 GTATCAA 26975 0.0 22.64406 1 CATGGGA 11720 0.0 22.133396 4 GGTATCA 20685 0.0 21.653643 1 AGTACTT 12895 0.0 21.555305 12-13 GTACTTT 13500 0.0 21.379177 14-15 ATGGGAG 4335 0.0 20.565952 5 CCGTCGT 140 4.930179E-4 20.310198 9 AGTACAT 12915 0.0 19.314331 2 ATCAACG 31510 0.0 19.203953 3 TCAACGC 31655 0.0 19.130968 4 CAACGCA 31620 0.0 19.121784 5 TATCAAC 31850 0.0 19.118065 2 GTAAGGT 945 0.0 19.069536 4 ATGGGGA 3315 0.0 18.882978 5 AACGCAG 32045 0.0 18.87077 6 >>END_MODULE