FastQCFastQC Report
Fri 27 May 2016
SRR939336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631394
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168790.6414470809008457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133120.5058915540584192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.36839789100377973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64960.24686534969677668No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45080.1713160400912976No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39240.14912248032791744No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30660.11651618875774589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.11590814602450261No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28380.10785157980902897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28060.1066354943425424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG10300.026.25885
GTACATG169350.025.9299871
TACATGG170500.025.297452
ACATGGG172000.024.1940383
CATGGGG57650.023.046024
GTATCAA276850.022.8556181
GAGTACT140300.022.50965912-13
AGTACTT143950.022.13669812-13
GTACTTT147900.021.16046114-15
CATGGGA110650.020.9698184
GGTATCA212950.020.4869611
GTAAGGT9900.019.6510564
AGGTAAG8500.019.538282
TACTTTT163850.019.31781214-15
ATGGGAT47050.019.2624245
ATCAACG327600.019.177033
TATCAAC329200.019.112652
TCAACGC329250.019.0809254
CAACGCA331450.018.9685925
ACTTTTT166800.018.90359516-17
Produced by FastQC (version 0.11.5)