##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2631394 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.265729115442234 33.0 31.0 34.0 31.0 34.0 2 32.45624980523631 34.0 31.0 34.0 31.0 34.0 3 32.535338303575976 34.0 31.0 34.0 31.0 34.0 4 36.08454264165685 37.0 35.0 37.0 35.0 37.0 5 36.02688726963731 37.0 35.0 37.0 35.0 37.0 6 36.07792181634525 37.0 35.0 37.0 35.0 37.0 7 36.01991833986092 37.0 35.0 37.0 35.0 37.0 8 36.00101239115085 37.0 35.0 37.0 35.0 37.0 9 37.69046520589467 39.0 37.0 39.0 35.0 39.0 10-11 37.611784096186284 39.0 37.0 39.0 35.0 39.0 12-13 37.53437056556335 39.0 37.0 39.0 35.0 39.0 14-15 38.78976941499448 40.0 38.0 41.0 34.5 41.0 16-17 38.75097799873375 40.0 38.0 41.0 34.5 41.0 18-19 38.89771182118679 40.0 38.0 41.0 35.0 41.0 20-21 38.90641367275292 40.0 38.0 41.0 35.0 41.0 22-23 38.84726935609035 40.0 38.0 41.0 35.0 41.0 24-25 38.77254755464214 40.0 38.0 41.0 34.0 41.0 26-27 38.59202707766302 40.0 38.0 41.0 34.0 41.0 28-29 38.4368351907772 40.0 38.0 41.0 34.0 41.0 30-31 38.21394990639942 40.0 37.5 41.0 34.0 41.0 32-33 37.974455174709675 40.0 37.0 41.0 33.0 41.0 34-35 37.89265575584652 40.0 37.0 41.0 33.0 41.0 36-37 37.77709077393959 40.0 37.0 41.0 33.0 41.0 38-39 37.652431752903595 40.0 37.0 41.0 33.0 41.0 40-41 37.44956665554455 40.0 37.0 41.0 33.0 41.0 42-43 37.23467561300208 40.0 36.0 41.0 32.0 41.0 44-45 36.948847835025845 40.0 36.0 41.0 31.0 41.0 46-47 36.561517203429055 40.0 36.0 41.0 30.5 41.0 48-49 36.16608478243851 39.5 35.0 41.0 29.5 41.0 50-51 35.9033341263224 39.0 35.0 41.0 29.0 41.0 52-53 35.6638072823758 39.0 35.0 41.0 28.0 41.0 54-55 35.384055181398146 39.0 35.0 40.0 27.5 41.0 56-57 35.14260217208066 38.0 35.0 40.0 27.0 41.0 58-59 34.85839881826895 38.0 34.0 40.0 26.0 41.0 60-61 34.52115323664947 38.0 34.0 40.0 26.0 41.0 62-63 34.157742436138406 37.0 34.0 40.0 25.5 41.0 64-65 33.73905067048112 36.5 33.5 39.5 24.5 41.0 66-67 33.36703910550834 36.0 33.0 39.0 23.0 40.5 68-69 32.98956560667084 36.0 33.0 39.0 22.5 40.0 70-71 32.60202862057146 35.0 32.5 38.5 22.0 40.0 72-73 32.123966802386875 35.0 32.0 37.5 20.5 39.5 74-75 31.639497163860675 35.0 31.0 37.0 18.5 39.0 76-77 29.658999564489392 33.0 28.5 35.0 13.0 37.5 78-79 30.62453475230239 34.0 30.5 36.0 14.0 38.0 80-81 30.675387076203716 34.0 31.0 36.0 14.0 37.0 82-83 30.378519142325324 34.0 31.0 35.0 9.0 37.0 84-85 30.016296115290984 34.0 30.5 35.0 6.0 36.5 86-87 29.49896233707305 34.0 30.0 35.0 2.0 36.0 88-89 29.007042464944433 34.0 29.0 35.0 2.0 36.0 90-91 28.598654553442017 34.0 29.0 35.0 2.0 35.0 92-93 28.27418052940761 34.0 29.0 35.0 2.0 35.0 94-95 27.903138032540927 34.0 28.5 35.0 2.0 35.0 96-97 27.38261621026726 33.0 27.0 35.0 2.0 35.0 98-99 26.68035003500046 33.0 26.0 35.0 2.0 35.0 100-101 23.436000652125834 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24637963025747922 1101 2 0.1942915810689385 1101 3 0.1883686162239968 1101 4 0.1740158256547133 1101 5 0.16809665061796153 1101 6 0.14377117164021058 1101 7 0.19666287006606353 1101 8 0.21550656342683538 1101 9 0.21365357100668803 1101 10-11 0.2708229452029798 1101 12-13 0.2896580573466352 1101 14-15 0.3407000402634779 1101 16-17 0.29909853127094976 1101 18-19 0.2503024094009447 1101 20-21 0.2818146740138374 1101 22-23 0.2749009559689881 1101 24-25 0.30894647298487143 1101 26-27 0.3978778678533814 1101 28-29 0.35363816346288246 1101 30-31 0.38895838863248144 1101 32-33 0.3564180803376118 1101 34-35 0.44182727734020233 1101 36-37 0.5161154912240136 1101 38-39 0.6081579155865953 1101 40-41 0.6541408692240154 1101 42-43 0.5637415891840689 1101 44-45 0.7464277694893724 1101 46-47 0.8288163287500083 1101 48-49 0.8301135557694082 1101 50-51 0.8432086830164423 1101 52-53 0.8015534107475801 1101 54-55 0.8004042874982034 1101 56-57 0.8853466988698173 1101 58-59 0.7776874841235255 1101 60-61 0.7842925202837279 1101 62-63 0.856928947357126 1101 64-65 0.7814968432576848 1101 66-67 0.8001019557585423 1101 68-69 0.8987152179918567 1101 70-71 0.8547969273244718 1101 72-73 0.7602146526752804 1101 74-75 0.6812299955967092 1101 76-77 0.7897653616872446 1101 78-79 1.0049774654070696 1101 80-81 1.0334420526738732 1101 82-83 0.9849746484768787 1101 84-85 0.9427824363535606 1101 86-87 0.935342337282659 1101 88-89 1.0250864890489169 1101 90-91 0.9840947938551707 1101 92-93 1.0722109863828422 1101 94-95 1.1565064810178889 1101 96-97 1.061743971171925 1101 98-99 0.9728343639147674 1101 100-101 0.6676891558165678 1102 1 0.07665606961277405 1102 2 0.11837959573175283 1102 3 0.17022848811334512 1102 4 0.09871491678711664 1102 5 0.14193127828456653 1102 6 0.16974154369016503 1102 7 0.10051412566382112 1102 8 0.18541832201950825 1102 9 0.22933295426160782 1102 10-11 0.22783945976186715 1102 12-13 0.16825753205517202 1102 14-15 0.24849882340124196 1102 16-17 0.2686430582252086 1102 18-19 0.20155281092769428 1102 20-21 0.2347857829834723 1102 22-23 0.17010238068036188 1102 24-25 0.20199136839896425 1102 26-27 0.23098587382430935 1102 28-29 0.29635097263848564 1102 30-31 0.26156717876153124 1102 32-33 0.2035017679592599 1102 34-35 0.16874411878909967 1102 36-37 0.31736239710614456 1102 38-39 0.20907150703077804 1102 40-41 0.25990065230509884 1102 42-43 0.3117423638944956 1102 44-45 0.4371088533281551 1102 46-47 0.4144531029313612 1102 48-49 0.40504940596685657 1102 50-51 0.2313814056512129 1102 52-53 0.35477422211503296 1102 54-55 0.42653923827575113 1102 56-57 0.388561369239973 1102 58-59 0.39906769688546007 1102 60-61 0.1628794295670204 1102 62-63 0.20239594658241344 1102 64-65 0.17976375934331656 1102 66-67 0.18765859464900103 1102 68-69 0.30486500163979713 1102 70-71 0.07925538583967295 1102 72-73 0.17244402364819678 1102 74-75 0.23929246371076118 1102 76-77 0.34211991452816193 1102 78-79 0.34408777661392165 1102 80-81 0.34645142478052193 1102 82-83 0.2818331976898847 1102 84-85 0.33803365038149025 1102 86-87 0.3825339175029754 1102 88-89 0.3883637975915164 1102 90-91 0.597239456856137 1102 92-93 0.46033788773008055 1102 94-95 0.3830874571530316 1102 96-97 0.25533088045522234 1102 98-99 0.42448052909815104 1102 100-101 0.388804266448993 1103 1 0.17519152251186654 1103 2 0.15620320880262994 1103 3 0.18872217980210593 1103 4 0.15855254187243872 1103 5 0.11367431165890451 1103 6 0.16809254565757215 1103 7 0.15034715902698537 1103 8 0.2005436335807076 1103 9 0.17794700269701735 1103 10-11 0.2713499374908963 1103 12-13 0.16877172673302 1103 14-15 0.33887056297127316 1103 16-17 0.2570447702337333 1103 18-19 0.2306468451522221 1103 20-21 0.22986169163613113 1103 22-23 0.20814356988053362 1103 24-25 0.28130052089665014 1103 26-27 0.2774287415168004 1103 28-29 0.3162319565406406 1103 30-31 0.48029347301831393 1103 32-33 0.4100792703081808 1103 34-35 0.43823352151888884 1103 36-37 0.4789477254924819 1103 38-39 0.4472212887843128 1103 40-41 0.5251823728937737 1103 42-43 0.475734994794891 1103 44-45 0.5215965411446746 1103 46-47 0.59690004396181 1103 48-49 0.5409238832534768 1103 50-51 0.642825128007317 1103 52-53 0.5747654743815716 1103 54-55 0.6371830960373828 1103 56-57 0.5663649172670446 1103 58-59 0.5195724735054412 1103 60-61 0.473610541221845 1103 62-63 0.43502030013831927 1103 64-65 0.5302174572437508 1103 66-67 0.6341234154657016 1103 68-69 0.47186096516968234 1103 70-71 0.5687142552928748 1103 72-73 0.4351729254668939 1103 74-75 0.43956724085582266 1103 76-77 0.4748150990293709 1103 78-79 0.6071437408811597 1103 80-81 0.5216490866890027 1103 82-83 0.5042256078301044 1103 84-85 0.42696558899317694 1103 86-87 0.4855077558587162 1103 88-89 0.7288726743624281 1103 90-91 0.7378478209406012 1103 92-93 0.5648073874111184 1103 94-95 0.5169760983942915 1103 96-97 0.4861562508932451 1103 98-99 0.4752277173665789 1103 100-101 0.183635841856578 1104 1 0.15774960564714746 1104 2 0.14051981915115164 1104 3 0.12298624451676687 1104 4 0.10578594688340814 1104 5 0.13096694503050088 1104 6 0.07098929213970706 1104 7 0.1483391074190834 1104 8 0.14179222562718508 1104 9 0.1673861386851172 1104 10-11 0.2494707439451176 1104 12-13 0.24034076630662327 1104 14-15 0.2626392436177767 1104 16-17 0.17493345971868024 1104 18-19 0.23890189896434322 1104 20-21 0.1809150294964752 1104 22-23 0.217236659186554 1104 24-25 0.19497828412333007 1104 26-27 0.2380998166274111 1104 28-29 0.21799237000741556 1104 30-31 0.09826246301038566 1104 32-33 0.20269189311675717 1104 34-35 0.23638930450272255 1104 36-37 0.2512836618556804 1104 38-39 0.3635813045210625 1104 40-41 0.37239125660894246 1104 42-43 0.472014763917457 1104 44-45 0.37047799280537674 1104 46-47 0.37253476827238075 1104 48-49 0.18999961902708407 1104 50-51 0.14247994361255678 1104 52-53 0.10582695007854426 1104 54-55 0.14489248365170226 1104 56-57 0.25358204660475536 1104 58-59 0.33540191307256606 1104 60-61 0.25323199708620336 1104 62-63 0.263915548396227 1104 64-65 0.19346111269528876 1104 66-67 0.15409156472764352 1104 68-69 0.2632038698923225 1104 70-71 0.23077496032054512 1104 72-73 0.31949411671683237 1104 74-75 0.30540099185957814 1104 76-77 0.36807362893726747 1104 78-79 0.39633604712250303 1104 80-81 0.41580601220354296 1104 82-83 0.3407731171670605 1104 84-85 0.44622423746558226 1104 86-87 0.48207827748865384 1104 88-89 0.6938883302666703 1104 90-91 0.6897127867455168 1104 92-93 0.7855443197161733 1104 94-95 0.8256218761505139 1104 96-97 0.8582620643266061 1104 98-99 1.066635293638587 1104 100-101 0.9340618656880437 1105 1 0.0705774697361008 1105 2 0.10931434689342012 1105 3 0.07668126688018617 1105 4 0.09882164656356451 1105 5 0.1385769648756181 1105 6 0.15416399370966616 1105 7 0.13293998818051733 1105 8 0.11716534389286437 1105 9 0.1477665966294026 1105 10-11 0.09559707251304417 1105 12-13 0.11049905368624735 1105 14-15 0.1243037363620374 1105 16-17 0.1280365725172672 1105 18-19 0.11299993640746209 1105 20-21 0.24082101273359768 1105 22-23 0.1760896891178092 1105 24-25 0.25761221711486115 1105 26-27 0.20301357002282572 1105 28-29 0.2844773742476363 1105 30-31 0.22708221686210806 1105 32-33 0.2614863778110532 1105 34-35 0.39452257240314026 1105 36-37 0.311939971181161 1105 38-39 0.23907926189496465 1105 40-41 0.209044449261512 1105 42-43 0.21886300691463134 1105 44-45 0.15185630265605 1105 46-47 0.19361277549582923 1105 48-49 0.12423997305356949 1105 50-51 0.06601512886949479 1105 52-53 0.01479642476418519 1105 54-55 -0.04685220745759722 1105 56-57 -0.07745037282021627 1105 58-59 0.007848630449743155 1105 60-61 -0.03466248373377567 1105 62-63 -0.08633951081625213 1105 64-65 -0.08394731351403095 1105 66-67 -0.040696187264408934 1105 68-69 0.02041461983098003 1105 70-71 -0.04390809901246939 1105 72-73 0.11888843180214081 1105 74-75 0.04005331494319364 1105 76-77 0.19147235213452518 1105 78-79 0.08527788501246292 1105 80-81 0.1320729784840715 1105 82-83 0.12199946768944514 1105 84-85 0.15545630475816807 1105 86-87 0.14329162752459723 1105 88-89 0.3440379219566907 1105 90-91 0.42852698740932027 1105 92-93 0.4659686865881767 1105 94-95 0.3347329786520312 1105 96-97 0.2877684332735235 1105 98-99 0.30162554327375446 1105 100-101 0.4806634438265647 1106 1 0.1499665867792217 1106 2 0.1454126791737309 1106 3 0.16508126951273994 1106 4 0.085310418742786 1106 5 0.06413901405162648 1106 6 0.09670943808391286 1106 7 0.0830013594752046 1106 8 0.042559239012121 1106 9 0.14688238928985697 1106 10-11 0.19775403494656985 1106 12-13 0.19244198989959216 1106 14-15 0.14743882231476135 1106 16-17 0.061800245317776614 1106 18-19 0.052436258341863606 1106 20-21 0.13344057981093727 1106 22-23 0.1604224860693222 1106 24-25 0.17880851311865342 1106 26-27 0.19230024800945245 1106 28-29 0.19606848676277622 1106 30-31 0.2375456935377187 1106 32-33 0.21072688747251078 1106 34-35 0.2796028486891444 1106 36-37 0.2607667593399512 1106 38-39 0.23461067478391584 1106 40-41 0.2539962900712851 1106 42-43 0.3619616978797211 1106 44-45 0.4224718748405394 1106 46-47 0.5252676944133299 1106 48-49 0.33042152678390124 1106 50-51 0.3573694271635972 1106 52-53 0.5124400467565025 1106 54-55 0.45579621893633515 1106 56-57 0.5060612334294561 1106 58-59 0.5022429467768106 1106 60-61 0.30199353756354697 1106 62-63 0.3592265365433036 1106 64-65 0.4206338973267947 1106 66-67 0.40005945297146894 1106 68-69 0.39968790472541116 1106 70-71 0.44348561182836477 1106 72-73 0.5738354023965684 1106 74-75 0.455845728595591 1106 76-77 0.5416459358232544 1106 78-79 0.5920743856969288 1106 80-81 0.5402428469882565 1106 82-83 0.4276902978637196 1106 84-85 0.3913868120503281 1106 86-87 0.6787773985981538 1106 88-89 0.7344667148014246 1106 90-91 0.6154210898417922 1106 92-93 0.6598231658700193 1106 94-95 0.641928721820566 1106 96-97 0.5793408217850811 1106 98-99 0.5744425021533424 1106 100-101 0.5665394902647272 1107 1 0.07606674607830399 1107 2 0.10384796775014848 1107 3 0.07557222469455382 1107 4 -0.07744557029008803 1107 5 0.03684372869182795 1107 6 0.08359646210131189 1107 7 0.13801171774497334 1107 8 0.14972712339774574 1107 9 0.04340786948468889 1107 10-11 0.13404277793894437 1107 12-13 0.10017560621854926 1107 14-15 0.11540931943512334 1107 16-17 0.08724382242466078 1107 18-19 0.1537407589239166 1107 20-21 0.16471235898259096 1107 22-23 0.16942063460039947 1107 24-25 0.06028865434341668 1107 26-27 0.12511844410049378 1107 28-29 -0.009787286297296305 1107 30-31 0.08675460701075366 1107 32-33 0.033816830186104596 1107 34-35 -0.03980449936403829 1107 36-37 0.004839713342342122 1107 38-39 -0.004482986184733306 1107 40-41 -0.01908173393079693 1107 42-43 -0.08137823143131584 1107 44-45 -0.05852496023895526 1107 46-47 -0.11061183948368125 1107 48-49 -0.004542133112558133 1107 50-51 -0.2073183025946932 1107 52-53 -0.15372761361478382 1107 54-55 -0.1526398833673781 1107 56-57 -0.23203369695378484 1107 58-59 -0.21158831107326392 1107 60-61 -0.39706593728558204 1107 62-63 -0.22868305548255563 1107 64-65 -0.221502481452724 1107 66-67 -0.334103020606328 1107 68-69 -0.32223754636670776 1107 70-71 -0.39110086153498713 1107 72-73 -0.31449895527582683 1107 74-75 -0.3102208381262166 1107 76-77 -0.22070405115711011 1107 78-79 -0.05245530864795356 1107 80-81 -0.15871515128089797 1107 82-83 -0.11096677447950754 1107 84-85 -0.1466989758674515 1107 86-87 -0.08937637862820225 1107 88-89 0.04803724485380911 1107 90-91 0.08603763665121633 1107 92-93 0.09386769685675489 1107 94-95 0.11550938995768689 1107 96-97 0.10378854028521545 1107 98-99 0.29907144250809026 1107 100-101 0.4455937668324452 1108 1 -0.053116587216955224 1108 2 -0.022972530661050428 1108 3 0.031107829791210406 1108 4 -0.011157505461753203 1108 5 -0.004773080864289625 1108 6 0.01211351098678648 1108 7 -0.008710810206608244 1108 8 -0.05195374083785964 1108 9 0.010078876518370805 1108 10-11 0.013432887841602792 1108 12-13 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1651.0 10 2796.0 11 4416.0 12 6630.0 13 9311.0 14 11391.0 15 15583.0 16 21216.0 17 30642.0 18 28147.0 19 16581.0 20 14443.0 21 14804.0 22 15607.0 23 16967.0 24 19442.0 25 23003.0 26 27237.0 27 32861.0 28 40288.0 29 48945.0 30 59418.0 31 72727.0 32 89897.0 33 115426.0 34 157562.0 35 230087.0 36 357944.0 37 582823.0 38 514681.0 39 47355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.650613400847945 19.651500520916297 14.750428889019298 21.94745718921646 2 15.531881580637489 21.531667245574017 39.43255932026903 23.503891853519466 3 19.28586141033992 26.001085356278843 30.330995662375155 24.38205757100609 4 12.588612727702502 17.075170042950617 37.161937740984435 33.17427948836244 5 12.9926951266135 38.74360890083355 33.49905031325602 14.764645659296935 6 30.757347626391184 36.78365915556545 17.373224990252314 15.08576822779105 7 28.07595517812992 34.316259746735 20.326982580335745 17.280802494799335 8 26.74563368313525 34.39826951038119 19.695302185837622 19.160794620645937 9 27.40855227305375 16.75666965874362 20.59227922538396 35.24249884281868 10-11 25.0307251593642 27.05900370678051 26.666816143838588 21.2434549900167 12-13 26.45742142757793 24.77211698438166 27.481707414397082 21.288754173643323 14-15 23.012369597813727 25.66260680670862 26.937076129357372 24.387947466120277 16-17 21.28553936378582 29.36605839221883 27.452574278571912 21.89582796542344 18-19 21.202051080149914 27.207746160400152 30.74313462750162 20.847068131948312 20-21 22.34697654551162 26.11383928062464 30.45959670045611 21.07958747340763 22-23 21.74813045860863 26.28479429534308 30.484982484569016 21.482092761479276 24-25 21.739764915120904 26.534322164331396 30.764120863878023 20.96179205666967 26-27 21.427831724311435 26.555844400643696 30.859891679608324 21.15643219543654 28-29 21.026440738255083 26.922574118509047 31.065701297487188 20.98528384574868 30-31 21.07105587380681 26.67515773008527 30.806485079771406 21.44730131633651 32-33 21.02552867415522 26.872068568979028 30.544114640376925 21.55828811648883 34-35 21.77590342123275 26.67142476560092 30.30926122913249 21.243410584033843 36-37 21.950180719754574 26.208317479889725 30.51877535521533 21.32272644514037 38-39 21.518556052391997 26.597543671555623 30.5911914452835 21.292708830768888 40-41 21.386243911134827 26.65213948150264 30.69483617862052 21.26678042874201 42-43 21.3995697484638 26.88872576414683 29.93676203129955 21.77494245608981 44-45 21.345068131654312 26.994146788600336 29.784283963979302 21.876501115766047 46-47 21.794845728902644 27.242782855680296 29.08044245048388 21.881928964933177 48-49 21.89198956902691 27.31459066943225 28.854249876681333 21.939169884859506 50-51 21.800061108294692 27.557712756052492 28.80881768370681 21.833408451946003 52-53 22.071533187352408 27.681639465621643 28.554959842577738 21.69186750444821 54-55 21.297437783927453 27.541827639646517 28.783830927637595 22.37690364878844 56-57 21.76656175396007 27.946556083961582 28.27294202236533 22.01394013971302 58-59 21.966934136763367 27.11870880139334 28.61246617512568 22.301890886717622 60-61 21.587419036038856 27.438429196069457 29.1244139631492 21.84973780474249 62-63 21.700253214971262 27.41657264054609 28.983157385094632 21.900016759388013 64-65 21.38298179596062 27.503368936768872 28.890010389930204 22.2236388773403 66-67 21.333027285157602 27.759620946160098 28.507950538763865 22.39940122991844 68-69 21.463618181009757 27.548137234298466 28.750280745904337 22.237963838787437 70-71 21.52139161246693 27.745109197845174 28.48562938230257 22.247869807385324 72-73 21.62304466757924 27.49035302201039 28.370589885057125 22.516012425353253 74-75 21.284839898548068 28.122983483279206 28.061134136507114 22.53104248166561 76-77 21.48626694372123 27.529402129714104 28.437587492167367 22.5467434343973 78-79 21.41364995131858 27.6509903115991 28.375701244283448 22.559658492798874 80-81 21.371402403512256 28.05036943858826 28.194078531708445 22.384149626191043 82-83 21.491612430521617 27.76959664725237 28.138165550274874 22.600625371951143 84-85 21.19137232964733 27.638582439573856 28.110062575197787 23.059982655581035 86-87 21.44462810546158 27.394484209485153 28.144455202034518 23.016432483018754 88-89 21.34295145191904 27.338733560990363 28.025118171695507 23.293196815395092 90-91 21.064707059099263 27.3498213404056 28.37862294139084 23.2068486591043 92-93 21.21810720857462 27.338798370749494 28.323105547857903 23.11998887281798 94-95 20.88351269327208 27.04080042745404 28.364357447041378 23.711329432232496 96-97 20.965199434216235 26.827130410725264 28.337489558766187 23.870180596292307 98-99 20.882391614482664 27.03109074505756 27.933521167867674 24.152996472592093 100-101 20.804954795754334 26.55111937037079 28.642504531825384 24.001421302049486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2088.0 1 1371.0 2 924.5 3 1289.0 4 1112.5 5 1081.5 6 2055.0 7 4249.5 8 6509.5 9 7135.5 10 5994.5 11 4856.5 12 4787.0 13 5001.5 14 5233.0 15 5413.5 16 5509.0 17 5520.0 18 5662.5 19 6044.0 20 6580.0 21 7460.5 22 8632.5 23 10024.5 24 11524.5 25 13698.0 26 17007.0 27 20754.5 28 23448.5 29 26483.0 30 31344.0 31 36816.5 32 44599.5 33 50804.5 34 55098.0 35 58751.0 36 63807.0 37 70578.5 38 73652.5 39 74445.5 40 74654.0 41 78286.0 42 82513.0 43 85572.5 44 89669.5 45 93857.5 46 92819.0 47 94962.0 48 116235.5 49 116545.0 50 95790.0 51 86788.0 52 81733.0 53 81106.0 54 80911.0 55 75508.0 56 66961.0 57 59530.0 58 54438.0 59 48080.5 60 40009.0 61 33130.5 62 28016.0 63 21792.0 64 15883.0 65 12473.0 66 9106.5 67 7251.5 68 5378.5 69 3306.5 70 2512.5 71 2168.5 72 1916.0 73 1683.0 74 1365.5 75 1162.5 76 849.5 77 453.5 78 259.5 79 168.0 80 93.0 81 59.0 82 41.0 83 24.0 84 10.5 85 4.0 86 1.5 87 3.5 88 4.5 89 3.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2725551551763058 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0024131695975593164 16-17 6.080427332432923E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5201068331082307E-4 26-27 0.0012730894727281433 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0034582430453212252 36-37 0.019951402184545528 38-39 0.007239508792677949 40-41 0.017652240599469328 42-43 0.005415380592948073 44-45 6.840480748987039E-4 46-47 1.330093478969702E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.6102537286320484E-4 60-61 0.0020331428892822584 62-63 0.0012540881373142904 64-65 0.0 66-67 0.0 68-69 4.180293791047635E-4 70-71 1.9001335413852884E-5 72-73 0.0 74-75 0.0 76-77 0.23405844962783984 78-79 0.0 80-81 0.0013870974852112606 82-83 0.0 84-85 0.0 86-87 0.10484936881364022 88-89 0.3597902860613044 90-91 0.36134839556524034 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.5201068331082307E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2631394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.77162421297922 #Duplication Level Percentage of deduplicated Percentage of total 1 66.40449876303404 24.41801274565531 2 15.430676721584364 11.34822091516133 3 6.400452485493738 7.060651007688137 4 3.4190603630169707 5.028976113214095 5 2.133428793034834 3.9224820931313373 6 1.3221534095758283 2.9170636997298938 7 0.9245984770649199 2.379929142256689 8 0.6567328770178603 1.9319307649607567 9 0.47373222020927186 1.56779128612041 >10 2.447260498767449 16.375023739522437 >50 0.22289712346406412 5.726875269387911 >100 0.15007247280046268 10.292441348414542 >500 0.00945077222444818 2.3540868623881797 >1k 0.004569604152480223 2.9052692461923777 >5k 2.0770927965819194E-4 0.618280523369454 >10k+ 2.0770927965819194E-4 1.1529652428071147 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16879 0.6414470809008457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13312 0.5058915540584192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9694 0.36839789100377973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6496 0.24686534969677668 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4508 0.1713160400912976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3924 0.14912248032791744 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3066 0.11651618875774589 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3050 0.11590814602450261 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2838 0.10785157980902897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2806 0.1066354943425424 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.800267082770577E-5 0.0 16-17 0.0 0.0 0.0 3.800267082770577E-5 0.0 18-19 0.0 0.0 0.0 5.700400624155865E-5 0.0 20-21 0.0 0.0 0.0 1.330093478969702E-4 0.0 22-23 0.0 0.0 0.0 3.800267082770577E-4 0.0 24-25 0.0 0.0 0.0 7.980560873818212E-4 0.0 26-27 0.0 0.0 0.0 0.0014060988206251136 0.0 28-29 0.0 0.0 0.0 0.0039522777660814 0.0 30-31 0.0 0.0 0.0 0.01257888404397061 0.0 32-33 0.0 0.0 0.0 0.02785595771670833 0.0 34-35 0.0 0.0 0.0 0.049916508132191534 0.0 36-37 0.0 0.0 0.0 0.0882041989911051 0.0 38-39 0.0 0.0 0.0 0.14803940420932782 0.0 40-41 0.0 0.0 0.0 0.21663422505333674 0.0 42-43 0.0 0.0 0.0 0.28686316074293705 0.0 44-45 0.0 0.0 0.0 0.37064004858261435 0.0 46-47 0.0 0.0 0.0 0.47833961770833255 0.0 48-49 0.0 0.0 0.0 0.5994541296362308 0.0 50-51 0.0 0.0 0.0 0.7333185376268244 0.0 52-53 0.0 0.0 0.0 0.8675819736611089 0.0 54-55 0.0 0.0 0.0 1.0116881014397692 0.0 56-57 0.0 0.0 0.0 1.189179575540569 0.0 58-59 0.0 0.0 0.0 1.3898146761754417 0.0 60-61 0.0 0.0 0.0 1.618780767912369 1.9001335413852884E-5 62-63 0.0 0.0 0.0 1.8485069130658502 3.800267082770577E-5 64-65 0.0 0.0 0.0 2.0819953226312746 3.800267082770577E-5 66-67 0.0 0.0 0.0 2.3589207849527662 3.800267082770577E-5 68-69 0.0 0.0 0.0 2.670694696423265 3.800267082770577E-5 70-71 0.0 0.0 0.0 3.028052811551596 3.800267082770577E-5 72-73 0.0 0.0 0.0 3.385125906648719 3.800267082770577E-5 74-75 0.0 0.0 0.0 3.7532197762858774 3.800267082770577E-5 76-77 0.0 0.0 0.0 4.150556701124955 3.800267082770577E-5 78-79 0.0 0.0 0.0 4.610351015469368 3.800267082770577E-5 80-81 0.0 0.0 0.0 5.116945618938098 3.800267082770577E-5 82-83 0.0 0.0 0.0 5.631976815330582 3.800267082770577E-5 84-85 0.0 0.0 0.0 6.170550666300827 3.800267082770577E-5 86-87 0.0 0.0 0.0 6.741768811512073 3.800267082770577E-5 88-89 0.0 0.0 0.0 7.348861477984673 3.800267082770577E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1030 0.0 26.2588 5 GTACATG 16935 0.0 25.929987 1 TACATGG 17050 0.0 25.29745 2 ACATGGG 17200 0.0 24.194038 3 CATGGGG 5765 0.0 23.04602 4 GTATCAA 27685 0.0 22.855618 1 GAGTACT 14030 0.0 22.509659 12-13 AGTACTT 14395 0.0 22.136698 12-13 GTACTTT 14790 0.0 21.160461 14-15 CATGGGA 11065 0.0 20.969818 4 GGTATCA 21295 0.0 20.486961 1 GTAAGGT 990 0.0 19.651056 4 AGGTAAG 850 0.0 19.53828 2 TACTTTT 16385 0.0 19.317812 14-15 ATGGGAT 4705 0.0 19.262424 5 ATCAACG 32760 0.0 19.17703 3 TATCAAC 32920 0.0 19.11265 2 TCAACGC 32925 0.0 19.080925 4 CAACGCA 33145 0.0 18.968592 5 ACTTTTT 16680 0.0 18.903595 16-17 >>END_MODULE