FastQCFastQC Report
Fri 27 May 2016
SRR939335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4621392
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120430.2605924794953555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.18392726693602274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57870.12522201103044278No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG47990.1038431710618792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA187900.032.8065531
GGTATCA126450.030.9233531
GTACATG215650.028.4309431
TACATGG218850.027.5371572
ATCAACG232200.026.484873
TCAACGC234000.026.3011194
TATCAAC237600.026.0226352
CAACGCA235600.026.021885
ACATGGG225050.025.8093553
AACGCAG240050.025.5048036
CATGGGG103850.022.6002944
ACGCAGA271450.022.4323317
CGCAGAG274900.022.0818528
CATGGGA121600.021.3289344
GAGTACT121000.021.08686412-13
GCAGAGT294500.020.1925569
GTACTTT128550.019.81561314-15
AGTACTT130350.019.79240212-13
AGTACAT159400.019.0078332
AGAGTAC244150.018.8727510-11
Produced by FastQC (version 0.11.5)