##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4621392 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.939144526151427 33.0 31.0 34.0 30.0 34.0 2 31.996653605666864 33.0 31.0 34.0 30.0 34.0 3 32.058181171387325 34.0 31.0 34.0 30.0 34.0 4 35.5445058545131 37.0 35.0 37.0 33.0 37.0 5 35.571390827698664 37.0 35.0 37.0 33.0 37.0 6 35.662928182677426 37.0 35.0 37.0 33.0 37.0 7 35.59684398120739 37.0 35.0 37.0 33.0 37.0 8 35.58010768184131 37.0 35.0 37.0 33.0 37.0 9 37.2163672763531 39.0 37.0 39.0 34.0 39.0 10-11 37.10757224230275 39.0 37.0 39.0 33.0 39.0 12-13 37.01035770174873 39.0 37.0 39.0 33.0 39.0 14-15 38.26370799101223 40.0 38.0 41.0 33.0 41.0 16-17 38.188439543756516 40.0 38.0 41.0 33.0 41.0 18-19 38.10254572648241 40.0 38.0 41.0 33.0 41.0 20-21 38.069066852584676 40.0 38.0 41.0 33.0 41.0 22-23 38.07856929254216 40.0 38.0 41.0 33.0 41.0 24-25 37.997577786086964 40.0 38.0 41.0 33.0 41.0 26-27 37.91757753940804 40.0 38.0 41.0 33.0 41.0 28-29 37.77450192496114 40.0 38.0 41.0 32.5 41.0 30-31 37.60613934070081 40.0 37.5 41.0 32.0 41.0 32-33 37.408459074668414 40.0 37.0 41.0 31.5 41.0 34-35 37.12821007177058 40.0 37.0 41.0 31.0 41.0 36-37 36.899823473100746 40.0 36.0 41.0 30.0 41.0 38-39 36.31601777126892 39.5 35.5 40.5 29.5 41.0 40-41 36.29130487091335 39.0 35.0 40.0 30.0 41.0 42-43 36.49418540993709 39.5 36.0 41.0 30.0 41.0 44-45 36.41926393606082 40.0 36.0 41.0 30.0 41.0 46-47 36.164630375436666 39.5 35.5 41.0 29.0 41.0 48-49 35.93368632221634 39.0 35.0 41.0 28.0 41.0 50-51 34.79850400052625 38.0 34.0 40.0 25.5 40.5 52-53 34.901776564290586 38.5 34.5 39.5 26.5 40.5 54-55 35.38634474201712 39.0 35.0 40.5 27.0 41.0 56-57 35.29721055906965 39.0 35.0 41.0 27.0 41.0 58-59 35.08066487326762 38.5 35.0 40.0 26.0 41.0 60-61 34.77396128698885 38.0 34.0 40.0 26.0 41.0 62-63 34.385612927879734 37.5 34.0 40.0 25.5 41.0 64-65 33.9870275016705 37.0 34.0 40.0 24.5 41.0 66-67 33.62175584758877 36.5 33.5 39.0 23.5 41.0 68-69 33.21652426368505 36.0 33.0 39.0 22.5 41.0 70-71 32.79692828481116 35.0 33.0 38.5 22.0 40.0 72-73 32.35773279998753 35.0 32.0 37.5 20.5 40.0 74-75 31.901032848977103 35.0 32.0 37.0 20.0 39.0 76-77 31.44095729165585 35.0 31.0 36.5 18.5 39.0 78-79 31.052540987650474 35.0 31.0 36.0 17.5 38.5 80-81 30.659741805066524 35.0 31.0 36.0 12.0 37.0 82-83 30.24453508812929 34.0 30.5 35.0 7.5 37.0 84-85 29.84634618746906 34.0 30.0 35.0 4.5 36.5 86-87 29.423313365323693 34.0 30.0 35.0 2.0 36.0 88-89 29.011542626117844 34.0 29.0 35.0 2.0 36.0 90-91 28.616161537476152 34.0 29.0 35.0 2.0 35.0 92-93 28.1737315293747 34.0 29.0 35.0 2.0 35.0 94-95 27.690051395769935 34.0 27.5 35.0 2.0 35.0 96-97 27.080127048300596 33.5 26.5 35.0 2.0 35.0 98-99 26.275850760982838 33.0 25.0 35.0 2.0 35.0 100-101 23.477367100648465 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34830759292137614 1101 2 0.4044807674553894 1101 3 0.4178372614423722 1101 4 0.3426085476532279 1101 5 0.2565300657264231 1101 6 0.31700528935550665 1101 7 0.2641649322709725 1101 8 0.2566473515735552 1101 9 0.33578079986691733 1101 10-11 0.4478643647545013 1101 12-13 0.5718384413006703 1101 14-15 0.590563620944927 1101 16-17 0.6456298111498455 1101 18-19 0.5929218986368312 1101 20-21 0.5510334517183537 1101 22-23 0.6893003962813893 1101 24-25 0.7031514736803999 1101 26-27 0.6192604517619316 1101 28-29 0.7672079324281924 1101 30-31 0.6243423843333034 1101 32-33 0.664189897109523 1101 34-35 0.6800281403965371 1101 36-37 0.49781660013393747 1101 38-39 0.9256130258321278 1101 40-41 0.7628540196792315 1101 42-43 0.6818648266114522 1101 44-45 0.6056055566814038 1101 46-47 0.763061732555677 1101 48-49 0.678550897884918 1101 50-51 0.6559459627849265 1101 52-53 0.6979473991367939 1101 54-55 0.6547326662812125 1101 56-57 0.621988942144128 1101 58-59 0.6402713169934771 1101 60-61 0.6870568091353633 1101 62-63 0.7058844325250604 1101 64-65 0.5000042610965778 1101 66-67 0.5664666656050628 1101 68-69 0.7179050803337361 1101 70-71 0.6797346821785055 1101 72-73 0.5991089013235467 1101 74-75 0.5468404382457059 1101 76-77 0.49966275204388566 1101 78-79 0.6256999890995623 1101 80-81 0.6661260727429443 1101 82-83 0.6942786011777677 1101 84-85 0.6605320123572795 1101 86-87 0.6025409316500685 1101 88-89 0.4224304436560864 1101 90-91 0.6302241050474784 1101 92-93 0.6584475663258758 1101 94-95 0.7211882611667306 1101 96-97 0.6256937435564573 1101 98-99 0.555046627317271 1101 100-101 0.4892003150462294 1102 1 0.349407343779756 1102 2 0.33768856724537955 1102 3 0.3887635758999508 1102 4 0.3244213125338149 1102 5 0.32174664976994194 1102 6 0.37806888270247185 1102 7 0.4036221776297353 1102 8 0.3622842924747971 1102 9 0.444983636079165 1102 10-11 0.537478329338299 1102 12-13 0.5155442458259358 1102 14-15 0.6273995154926197 1102 16-17 0.6615589209722685 1102 18-19 0.5934428920675572 1102 20-21 0.6598533435758966 1102 22-23 0.6484227411145795 1102 24-25 0.6325979089197418 1102 26-27 0.5074576339791506 1102 28-29 0.6139561804255322 1102 30-31 0.6301107039442471 1102 32-33 0.5541542966080826 1102 34-35 0.4408959378622086 1102 36-37 0.49433365461020173 1102 38-39 0.9813360282121337 1102 40-41 0.7226128859370178 1102 42-43 0.6297246533332199 1102 44-45 0.7108334218959911 1102 46-47 0.6160468590141264 1102 48-49 0.5980545332345528 1102 50-51 0.5870157210632243 1102 52-53 0.62527762627996 1102 54-55 0.6779173467190915 1102 56-57 0.6065620868367532 1102 58-59 0.6313335418838477 1102 60-61 0.6004034213113272 1102 62-63 0.6299895891308225 1102 64-65 0.5743475566563845 1102 66-67 0.6334525299298122 1102 68-69 0.5416660941744027 1102 70-71 0.6484015819641655 1102 72-73 0.5703752669297586 1102 74-75 0.506006363951581 1102 76-77 0.4772046773591647 1102 78-79 0.4736824267814903 1102 80-81 0.41962801505733793 1102 82-83 0.31528360188950444 1102 84-85 0.4534711201648385 1102 86-87 0.35720279565298796 1102 88-89 0.32436290722866445 1102 90-91 0.4128305059860047 1102 92-93 0.38999841617868114 1102 94-95 0.4352345569472753 1102 96-97 0.4513195373816252 1102 98-99 0.536356192141195 1102 100-101 0.37333665389533266 1103 1 0.28701955793149736 1103 2 0.3953978535005547 1103 3 0.3894776376589064 1103 4 0.3664034691961504 1103 5 0.3394997746744366 1103 6 0.37854298077291304 1103 7 0.4013105462954627 1103 8 0.347825687908923 1103 9 0.4520684507921118 1103 10-11 0.4773805554542676 1103 12-13 0.4585521623638442 1103 14-15 0.6080290126894639 1103 16-17 0.6171084774356075 1103 18-19 0.602448802871244 1103 20-21 0.6519806386531286 1103 22-23 0.6693760987385744 1103 24-25 0.6803416277004075 1103 26-27 0.7215665084475731 1103 28-29 0.6455713680895911 1103 30-31 0.656442021356213 1103 32-33 0.7185992617284569 1103 34-35 0.616141900642937 1103 36-37 0.4016640076398019 1103 38-39 0.8276001218342017 1103 40-41 0.5866875203036628 1103 42-43 0.6334706053608556 1103 44-45 0.6793300663660133 1103 46-47 0.6221113752892151 1103 48-49 0.5436399936988323 1103 50-51 0.5199217888620211 1103 52-53 0.5939314922421275 1103 54-55 0.6187305293280545 1103 56-57 0.5311739568896314 1103 58-59 0.5616249392988095 1103 60-61 0.6467148851237852 1103 62-63 0.5045360634190459 1103 64-65 0.5326257945532191 1103 66-67 0.5781300051495961 1103 68-69 0.44329739449461414 1103 70-71 0.3133506123184304 1103 72-73 0.4999696377438312 1103 74-75 0.37866700096462225 1103 76-77 0.33001165826506806 1103 78-79 0.49500266285143013 1103 80-81 0.47005417698528973 1103 82-83 0.40846560529200815 1103 84-85 0.566315968925462 1103 86-87 0.46987457626279294 1103 88-89 0.4379764038278573 1103 90-91 0.5078910458816672 1103 92-93 0.4527582306369453 1103 94-95 0.5422408458069619 1103 96-97 0.628964938964252 1103 98-99 0.6130309292847471 1103 100-101 0.3879171362449867 1104 1 0.2621090877926484 1104 2 0.28980316339741563 1104 3 0.2861319299549976 1104 4 0.2534137957401512 1104 5 0.2848436021204179 1104 6 0.2348543642664751 1104 7 0.3419055793404553 1104 8 0.39912808191854054 1104 9 0.5170225199969494 1104 10-11 0.4205268830978852 1104 12-13 0.38387994729275476 1104 14-15 0.4581179158897797 1104 16-17 0.49350341754198723 1104 18-19 0.5098817164198692 1104 20-21 0.5466651500491295 1104 22-23 0.5324624432345431 1104 24-25 0.5550987105515759 1104 26-27 0.5127734516842253 1104 28-29 0.6119447758546244 1104 30-31 0.6071918795655904 1104 32-33 0.5736915075789284 1104 34-35 0.45228769693326853 1104 36-37 0.3796930863547274 1104 38-39 0.8196229085990083 1104 40-41 0.5753133646774771 1104 42-43 0.45908420271626227 1104 44-45 0.46395685059334113 1104 46-47 0.4778900833101716 1104 48-49 0.5316511595551603 1104 50-51 0.45883684753538034 1104 52-53 0.4747611526084867 1104 54-55 0.5714749043240985 1104 56-57 0.5962726401306213 1104 58-59 0.5633768350510877 1104 60-61 0.6497813637830205 1104 62-63 0.6139537150909362 1104 64-65 0.5147235960617778 1104 66-67 0.4723025553120763 1104 68-69 0.4740063916637638 1104 70-71 0.6573326989089168 1104 72-73 0.4873526002270978 1104 74-75 0.5487705694054874 1104 76-77 0.5341941991915284 1104 78-79 0.4816907617773474 1104 80-81 0.43629027027979816 1104 82-83 0.40045566796635157 1104 84-85 0.5371498520216811 1104 86-87 0.39143132294830707 1104 88-89 0.3980113921609494 1104 90-91 0.47609154869778436 1104 92-93 0.5125680067110636 1104 94-95 0.5634580387355861 1104 96-97 0.5191755052645526 1104 98-99 0.6434224917581481 1104 100-101 0.31447248774937364 1105 1 0.2494788556778751 1105 2 0.27393295545009977 1105 3 0.3107361071916799 1105 4 0.34229295522500536 1105 5 0.28361241867924747 1105 6 0.3105559468094725 1105 7 0.2681956682004909 1105 8 0.25280874408883136 1105 9 0.36208979731571134 1105 10-11 0.45570300723169765 1105 12-13 0.4238068032697839 1105 14-15 0.49484035501004797 1105 16-17 0.4457566423328174 1105 18-19 0.5027408946050542 1105 20-21 0.5408526798692819 1105 22-23 0.5514801209974252 1105 24-25 0.5589329690592209 1105 26-27 0.5433299644054657 1105 28-29 0.5766065291312685 1105 30-31 0.5603795678997585 1105 32-33 0.5901877642196922 1105 34-35 0.533315487247414 1105 36-37 0.5578985265198 1105 38-39 0.9785051008693983 1105 40-41 0.8125002408973145 1105 42-43 0.6153774908305181 1105 44-45 0.4661854764749265 1105 46-47 0.5037818378895977 1105 48-49 0.5243886733632337 1105 50-51 0.4267740039204355 1105 52-53 0.4471776336616955 1105 54-55 0.5591308561143435 1105 56-57 0.5333687066283233 1105 58-59 0.4992565211775428 1105 60-61 0.639665098575442 1105 62-63 0.48024327399053846 1105 64-65 0.4961149255402333 1105 66-67 0.47237030174591865 1105 68-69 0.4173539104822339 1105 70-71 0.4748311813308419 1105 72-73 0.48609594602328343 1105 74-75 0.43961381179617476 1105 76-77 0.4471234901945458 1105 78-79 0.46885910781980655 1105 80-81 0.4358509983990828 1105 82-83 0.3492712090193528 1105 84-85 0.49349278046611644 1105 86-87 0.42556425209286175 1105 88-89 0.4225204557224309 1105 90-91 0.4083812796501469 1105 92-93 0.4428477599728602 1105 94-95 0.5365299375622357 1105 96-97 0.5867339243156486 1105 98-99 0.6875603996861095 1105 100-101 0.5267525403387268 1106 1 0.23214141421609114 1106 2 0.2627450821077204 1106 3 0.3120858786720859 1106 4 0.24045829005946473 1106 5 0.23055467022845022 1106 6 0.22744807835998415 1106 7 0.20752817813767876 1106 8 0.20193735785601774 1106 9 0.20256819463459408 1106 10-11 0.3930835894743012 1106 12-13 0.4592150422475356 1106 14-15 0.5275989205463958 1106 16-17 0.5202471433336342 1106 18-19 0.4701752182335355 1106 20-21 0.41785940619361384 1106 22-23 0.5374759149573904 1106 24-25 0.47153840926800683 1106 26-27 0.5050985523320364 1106 28-29 0.5481726292050908 1106 30-31 0.4987226595303156 1106 32-33 0.49907640374701856 1106 34-35 0.40400558381974605 1106 36-37 0.4068477257003238 1106 38-39 0.863839282479141 1106 40-41 0.7738201288918276 1106 42-43 0.6949073281437208 1106 44-45 0.5397117831368092 1106 46-47 0.48257109649306784 1106 48-49 0.527297275988623 1106 50-51 0.33899374124513315 1106 52-53 0.5473033561535843 1106 54-55 0.4745385458483824 1106 56-57 0.5628925686526713 1106 58-59 0.6474065405620237 1106 60-61 0.6910787623912 1106 62-63 0.6072404187279261 1106 64-65 0.7006038965049584 1106 66-67 0.534125616282509 1106 68-69 0.6946519522573453 1106 70-71 0.6551629345992964 1106 72-73 0.6132990068599753 1106 74-75 0.6362742741547684 1106 76-77 0.7006926690510404 1106 78-79 0.7713073566879736 1106 80-81 0.8533336926950561 1106 82-83 0.7493469764868586 1106 84-85 0.7645151342832897 1106 86-87 0.8863082640788491 1106 88-89 0.910089792712828 1106 90-91 0.7960690611501278 1106 92-93 0.735089858357501 1106 94-95 0.7807742204481052 1106 96-97 0.9351899609769667 1106 98-99 0.9160201875009868 1106 100-101 0.6830124299950597 1107 1 0.08489273449248103 1107 2 0.024821767410720952 1107 3 0.10653799560022748 1107 4 0.08559909014658018 1107 5 0.092637068358016 1107 6 0.07880704094134927 1107 7 0.11583328601808773 1107 8 0.08428188654016111 1107 9 0.16429992490023437 1107 10-11 0.16049390768257155 1107 12-13 0.25117134410869824 1107 14-15 0.29693405872395573 1107 16-17 0.3375834440138803 1107 18-19 0.3124573045731367 1107 20-21 0.27949599297527783 1107 22-23 0.12085030523776652 1107 24-25 0.2335500105234516 1107 26-27 0.2878123443222691 1107 28-29 0.3055698769262847 1107 30-31 0.31650525082243064 1107 32-33 0.3611778765827154 1107 34-35 0.3559254386142712 1107 36-37 0.23777508998617947 1107 38-39 0.6862406365340661 1107 40-41 0.5452045998189092 1107 42-43 0.2955396306888858 1107 44-45 0.36622742669630526 1107 46-47 0.4668385856022681 1107 48-49 0.41378312466542866 1107 50-51 0.4256655635141158 1107 52-53 0.4823307853893013 1107 54-55 0.4989511024111124 1107 56-57 0.44504980097607927 1107 58-59 0.36050273535944655 1107 60-61 0.3287352612490153 1107 62-63 0.36214443940759367 1107 64-65 0.36017234421878896 1107 66-67 0.2725716162687064 1107 68-69 0.3725347058857551 1107 70-71 0.35418448844211525 1107 72-73 0.3196448644472554 1107 74-75 0.38869714524502896 1107 76-77 0.41670853997336366 1107 78-79 0.48874798354991356 1107 80-81 0.4650322962348632 1107 82-83 0.5078883231649449 1107 84-85 0.4412747807168351 1107 86-87 0.6651002389761516 1107 88-89 0.6436814237669886 1107 90-91 0.5217657767732895 1107 92-93 0.5145736028737637 1107 94-95 0.38112021069565927 1107 96-97 0.5383831626366735 1107 98-99 0.6518439425840619 1107 100-101 0.39904028251671875 1108 1 0.09449215577999226 1108 2 0.1583177169790062 1108 3 0.13976265053086223 1108 4 0.07687283190075789 1108 5 0.1415542053983927 1108 6 0.07384630410178517 1108 7 0.17622102709425747 1108 8 0.19925329362290256 1108 9 0.33953347540512624 1108 10-11 0.345952644777789 1108 12-13 0.29436772104244824 1108 14-15 0.3355887281594434 1108 16-17 0.34980642986654686 1108 18-19 0.3512725370862597 1108 20-21 0.29401146019669966 1108 22-23 0.3280178465803303 1108 24-25 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2316 70-71 -1.1150957396092664 2316 72-73 -0.9271614853080763 2316 74-75 -1.020402191750037 2316 76-77 -1.0232913205423735 2316 78-79 -0.9578696732777949 2316 80-81 -0.8683619747687317 2316 82-83 -0.9266407486697581 2316 84-85 -0.9044883731503397 2316 86-87 -0.9083412516898868 2316 88-89 -1.0072045826012008 2316 90-91 -1.0131577118684518 2316 92-93 -1.1862084299190663 2316 94-95 -1.1238312688162608 2316 96-97 -1.0598671074310637 2316 98-99 -1.256201324915164 2316 100-101 -0.6782479491572495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15804.0 3 3604.0 4 3610.0 5 4267.0 6 5163.0 7 5923.0 8 7450.0 9 9384.0 10 10617.0 11 13503.0 12 17084.0 13 22334.0 14 22767.0 15 23323.0 16 28836.0 17 35305.0 18 31663.0 19 24225.0 20 25111.0 21 27558.0 22 29479.0 23 33012.0 24 37648.0 25 43348.0 26 50460.0 27 59403.0 28 70281.0 29 83979.0 30 101083.0 31 124548.0 32 156323.0 33 203304.0 34 278812.0 35 404955.0 36 637178.0 37 990114.0 38 869320.0 39 110597.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30096978864749 18.367408503799616 13.510305021697352 23.82131668585554 2 15.442217529492334 20.006746431512774 39.868873825038065 24.682162213956833 3 19.34111673916413 24.14314067037302 29.15848382925357 27.357258761209284 4 12.843725972411288 15.71359309401837 36.45512920310693 34.987551730463416 5 14.240548877280702 37.337377887035856 33.374529200417065 15.04754403526638 6 33.12692150385947 34.98304657361418 17.329083639791897 14.560948282734454 7 29.978946839306776 32.2096242215563 20.118497423549858 17.692931515587073 8 26.934351552616832 34.66846405108374 19.75965003443229 18.637534361867136 9 27.44307648634973 16.511204844471365 19.873774173870927 36.171944495307976 10-11 25.682110785287044 26.035294301179597 26.995913163720886 21.286681749812473 12-13 26.109733233328868 23.36254607549644 27.720972816377852 22.80674787479684 14-15 23.000550042193012 24.802179280819562 25.802753374493143 26.39451730249428 16-17 22.93524589817685 28.239684529049214 26.22205512986358 22.603014442910357 18-19 22.73923245265005 26.679148644055733 28.433079183054183 22.148539720240034 20-21 23.434049841856606 26.115166973091508 28.14461518620931 22.306167998842575 22-23 23.09090796772209 25.962689245063746 28.148634804130623 22.797767983083542 24-25 23.003093619217946 26.19813515618885 28.243709290418845 22.555061934174354 26-27 22.741386596267155 26.43257051177907 28.180553160602646 22.64548973135113 28-29 22.54055297776139 26.514810367663788 28.484056530045205 22.46058012452962 30-31 22.66077525572728 26.162288113965726 28.334939152441773 22.841997477865217 32-33 22.499255710706592 26.438272000319067 28.284976475906433 22.77749581306791 34-35 23.138632792862442 26.449124689056415 27.821109309903395 22.591133208177748 36-37 23.13433736334661 26.148362453117162 27.863240671618872 22.854059511917352 38-39 22.81231379344783 26.360376234634682 28.101816044689464 22.725493927228023 40-41 22.85980851620323 26.20678159374527 28.206746169601455 22.726663720450045 42-43 22.914085520914927 26.25116392713335 27.698656153942387 23.136094398009334 44-45 22.75271104216539 26.546844008664205 27.71912898830367 22.981315960866734 46-47 22.8658619102304 26.688735783110335 27.331602514596646 23.11379979206262 48-49 23.18841603789717 26.61968366046523 27.208761329075237 22.98313897256236 50-51 22.916051136456282 26.71780486219406 27.22778454996888 23.138359451380776 52-53 23.20074052815355 26.700464406381673 27.111505363719772 22.987289701745002 54-55 22.69525546294881 26.481305121041025 27.35126408167267 23.47217533433749 56-57 22.84661969316746 26.93919423184465 27.20880781902219 23.0053782559657 58-59 23.05091934277114 26.32425522474954 27.327222017077663 23.29760341540166 60-61 23.04247802063439 26.42488531985564 27.72181014154273 22.810826517967246 62-63 22.834295839527343 26.587858018333364 27.54605218459465 23.031793957544643 64-65 22.60786344587326 26.409368877200944 27.46951264584926 23.513255031076536 66-67 22.64879431998042 26.758217906794634 27.202566006179975 23.390421767044966 68-69 22.452431326419124 26.487729612769368 27.656083189714643 23.40375587109687 70-71 22.54267502683446 26.549302075730964 27.47799780845115 23.430025088983427 72-73 22.677644934728523 26.44349860599231 27.406584743122313 23.472271716156857 74-75 22.477947762585433 26.777591837076375 27.17460114523672 23.569859255101473 76-77 22.59514725857401 26.514379896024643 27.29437443759538 23.596098407805968 78-79 22.518832230541385 26.527365487366662 27.40657784559174 23.547224436500215 80-81 22.459234736282042 26.916462941775833 27.276799531229894 23.347502790712234 82-83 22.718583251320403 26.653704004603956 27.08824301404717 23.53946973002847 84-85 22.47526563580595 26.34004230782984 27.15447292319541 24.0302191331688 86-87 22.55008593520139 26.25966288706844 27.473244224766447 23.71700695296372 88-89 22.460416771740775 26.26353064656664 27.318286286220133 23.957766295472453 90-91 22.388054022255854 26.323570164583128 27.591860545895404 23.696515267265617 92-93 22.556533133169353 26.056568793901842 27.48046487678782 23.906433196140988 94-95 22.355901838927352 25.87080562852541 27.619548977795056 24.15374355475218 96-97 22.41515864345153 25.813107280335384 27.631497837755038 24.14023623845805 98-99 22.217628407157015 25.885685249476047 27.36661260629343 24.530073737073508 100-101 22.328634127370083 25.347244791681383 28.023126971604544 24.300994109343986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5404.0 1 3090.5 2 862.0 3 965.0 4 856.5 5 824.0 6 1276.5 7 2268.5 8 3518.0 9 4067.0 10 3841.5 11 3812.0 12 4223.0 13 4653.5 14 5007.5 15 5395.0 16 5690.5 17 5863.0 18 5991.5 19 6203.0 20 6645.0 21 7402.0 22 8649.0 23 10592.0 24 12757.5 25 15442.0 26 19355.0 27 24372.0 28 29171.0 29 34659.5 30 42035.5 31 50269.5 32 60262.0 33 68786.0 34 76544.5 35 85914.0 36 97078.0 37 107669.0 38 113930.5 39 117331.5 40 121964.5 41 134247.5 42 147374.5 43 156878.0 44 165387.0 45 173399.5 46 174312.0 47 175383.5 48 191266.5 49 194591.5 50 183078.0 51 176604.0 52 171435.0 53 170632.0 54 170761.5 55 158123.5 56 138149.0 57 125145.5 58 113847.5 59 98676.5 60 84475.5 61 71167.5 62 58367.0 63 46274.5 64 35936.5 65 28007.0 66 21696.0 67 18546.5 68 14160.5 69 9099.5 70 7015.0 71 5749.5 72 4843.0 73 4084.0 74 3133.5 75 2571.5 76 1823.0 77 1056.5 78 650.0 79 431.0 80 280.0 81 195.5 82 151.5 83 106.0 84 71.0 85 45.5 86 43.0 87 38.0 88 26.0 89 21.0 90 14.5 91 12.0 92 13.0 93 11.0 94 10.5 95 8.5 96 8.0 97 7.5 98 4.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29616617677098156 2 0.18572326260139801 3 0.1858098166093679 4 0.20569559994045084 5 0.21828920810007027 6 0.11286642639274054 7 0.12838123232134388 8 0.1414725260267902 9 0.14383112274396978 10-11 0.13169192312619227 12-13 0.12113233415386533 14-15 0.13613863528564554 16-17 0.11214153657599268 18-19 0.1400335656442907 20-21 0.1666056460910479 22-23 0.17086843098356513 24-25 0.194638325422297 26-27 0.1753259623940146 28-29 0.17205854859315114 30-31 0.16106618958097474 32-33 0.1716041400513092 34-35 0.3561048272901325 36-37 0.4766421026392048 38-39 0.4432971710688035 40-41 0.4329972441203862 42-43 0.4571241738419939 44-45 0.46124630847156006 46-47 0.46413504848755527 48-49 0.4760145860814231 50-51 0.46953385473467735 52-53 0.47544116577862255 54-55 0.4581952796906213 56-57 0.4205551054747141 58-59 0.4099738780003947 60-61 0.3645222045652046 62-63 0.4728986417945069 64-65 0.4712865733960677 66-67 0.47999607044803816 68-69 0.49116153747615443 70-71 0.4838693623046909 72-73 0.4774102694599376 74-75 0.4815756810934887 76-77 0.4580329909256778 78-79 0.44509316673417876 80-81 0.4417716566783341 82-83 0.45439772259094235 84-85 0.4557393097144756 86-87 0.45708089683800895 88-89 0.4644488067664461 90-91 0.4822681131572478 92-93 0.4867797408226785 94-95 0.48602239325294194 96-97 0.48367461578675863 98-99 0.48065604475880863 100-101 0.4629557501289655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4621392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.66421804367489 #Duplication Level Percentage of deduplicated Percentage of total 1 65.36313288674191 22.657618904037186 2 14.778589613095006 10.245765054526288 3 6.679090735879378 6.945763728060355 4 3.7026297934963175 5.133950659870532 5 2.25060625694474 3.900775301059574 6 1.4939559927201942 3.107208976758456 7 1.0550972131983023 2.560188389890578 8 0.7817485291933246 2.167896117702364 9 0.5591420883528468 1.7444000941252935 >10 2.909755325477962 18.083446832021654 >50 0.23853859853024414 5.7543260264884815 >100 0.1701479535250508 11.486718474512992 >500 0.012734634503684224 2.981803283728574 >1k 0.004642181782570597 2.6575759345496 >5k 1.2546437250190686E-4 0.31067969902227205 >10k+ 6.273218625095343E-5 0.26188252364563747 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12043 0.2605924794953555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8500 0.18392726693602274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5787 0.12522201103044278 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 4799 0.1038431710618792 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2457752988709895E-5 0.0 12-13 0.0 0.0 0.0 5.409625498118316E-5 0.0 14-15 0.0 0.0 0.0 6.491550597741979E-5 0.0 16-17 0.0 0.0 0.0 7.573475697365642E-5 0.0 18-19 0.0 0.0 0.0 9.737325896612969E-5 0.0 20-21 0.0 0.0 0.0 1.2983101195483958E-4 0.0 22-23 0.0 0.0 0.0 3.137582788908623E-4 0.0 24-25 0.0 0.0 0.0 6.816128127629078E-4 0.0 26-27 0.0 0.0 0.0 0.0010927443506198997 0.0 28-29 0.0 0.0 0.0 0.00298611327496131 0.0 30-31 0.0 0.0 0.0 0.010224192191443616 0.0 32-33 0.0 0.0 0.0 0.023791532940724353 0.0 34-35 1.0819250996236632E-5 0.0 0.0 0.043882882040735774 0.0 36-37 2.1638501992473264E-5 0.0 0.0 0.07943494081436936 0.0 38-39 2.1638501992473264E-5 0.0 0.0 0.13690680210637834 0.0 40-41 2.1638501992473264E-5 0.0 0.0 0.20189804284077179 0.0 42-43 2.1638501992473264E-5 0.0 0.0 0.2687826524995066 0.0 44-45 2.1638501992473264E-5 0.0 0.0 0.3485638093457556 0.0 46-47 2.1638501992473264E-5 0.0 0.0 0.44821993027209117 0.0 48-49 2.1638501992473264E-5 0.0 0.0 0.5571914263061866 0.0 50-51 2.1638501992473264E-5 0.0 0.0 0.6814938010019491 0.0 52-53 2.1638501992473264E-5 0.0 0.0 0.8067482697853807 0.0 54-55 2.1638501992473264E-5 0.0 0.0 0.9435685178837891 0.0 56-57 4.327700398494653E-5 0.0 0.0 1.1064847993851203 0.0 58-59 4.327700398494653E-5 0.0 0.0 1.292878855548285 0.0 60-61 4.327700398494653E-5 0.0 0.0 1.5014415570027386 0.0 62-63 4.327700398494653E-5 0.0 0.0 1.7172964336286558 0.0 64-65 4.327700398494653E-5 0.0 0.0 1.9410060864778407 0.0 66-67 4.327700398494653E-5 0.0 0.0 2.1974764313436297 2.1638501992473264E-5 68-69 4.327700398494653E-5 0.0 0.0 2.487583827556719 2.1638501992473264E-5 70-71 4.327700398494653E-5 0.0 0.0 2.815093374463798 2.1638501992473264E-5 72-73 4.327700398494653E-5 0.0 0.0 3.1600543732278066 2.1638501992473264E-5 74-75 4.327700398494653E-5 0.0 0.0 3.5081204970277353 2.1638501992473264E-5 76-77 4.327700398494653E-5 0.0 0.0 3.8931884592347936 2.1638501992473264E-5 78-79 4.327700398494653E-5 0.0 0.0 4.319596779498472 2.1638501992473264E-5 80-81 6.491550597741979E-5 0.0 0.0 4.796996229707412 2.1638501992473264E-5 82-83 8.655400796989305E-5 0.0 0.0 5.285290665669565 2.1638501992473264E-5 84-85 8.655400796989305E-5 0.0 0.0 5.783971582588103 2.1638501992473264E-5 86-87 8.655400796989305E-5 0.0 0.0 6.314645024702514 2.1638501992473264E-5 88-89 8.655400796989305E-5 0.0 0.0 6.893561507009144 2.1638501992473264E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18790 0.0 32.806553 1 GGTATCA 12645 0.0 30.923353 1 GTACATG 21565 0.0 28.430943 1 TACATGG 21885 0.0 27.537157 2 ATCAACG 23220 0.0 26.48487 3 TCAACGC 23400 0.0 26.301119 4 TATCAAC 23760 0.0 26.022635 2 CAACGCA 23560 0.0 26.02188 5 ACATGGG 22505 0.0 25.809355 3 AACGCAG 24005 0.0 25.504803 6 CATGGGG 10385 0.0 22.600294 4 ACGCAGA 27145 0.0 22.432331 7 CGCAGAG 27490 0.0 22.081852 8 CATGGGA 12160 0.0 21.328934 4 GAGTACT 12100 0.0 21.086864 12-13 GCAGAGT 29450 0.0 20.192556 9 GTACTTT 12855 0.0 19.815613 14-15 AGTACTT 13035 0.0 19.792402 12-13 AGTACAT 15940 0.0 19.007833 2 AGAGTAC 24415 0.0 18.87275 10-11 >>END_MODULE