FastQCFastQC Report
Fri 27 May 2016
SRR939335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4621392
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121980.26394644730418887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92120.1993338803546637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65460.14164563404272998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194100.032.5158961
GGTATCA131300.030.8491041
GTACATG208900.027.933781
TACATGG212700.026.8167692
ATCAACG243600.025.6748313
TCAACGC244000.025.652194
ACATGGG213800.025.569033
TATCAAC246700.025.4483832
CAACGCA246450.025.3201585
AACGCAG252750.024.6702566
CATGGGG100450.022.8174274
ACGCAGA288800.021.4264327
CGCAGAG291050.021.1629668
GAGTACT131850.021.03674512-13
CATGGGA119650.020.7423744
GCAGAGT311700.019.4871069
AGTACTT141850.019.45335612-13
GTACTTT140700.019.42685914-15
GCACCGT12800.018.9073076
AGAGTAC255150.018.85890810-11
Produced by FastQC (version 0.11.5)