##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939335_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4621392 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34191148467821 34.0 31.0 34.0 31.0 34.0 2 32.551223311071645 34.0 31.0 34.0 31.0 34.0 3 32.64652641455215 34.0 31.0 34.0 31.0 34.0 4 36.15905445805073 37.0 35.0 37.0 35.0 37.0 5 36.100933658083974 37.0 35.0 37.0 35.0 37.0 6 36.129929683523926 37.0 36.0 37.0 35.0 37.0 7 36.069552636954406 37.0 35.0 37.0 35.0 37.0 8 36.04017231171907 37.0 35.0 37.0 35.0 37.0 9 37.756044499146576 39.0 38.0 39.0 35.0 39.0 10-11 37.67841529132348 39.0 37.0 39.0 35.0 39.0 12-13 37.60464141540038 39.0 37.0 39.0 35.0 39.0 14-15 38.93027317310455 40.0 38.0 41.0 35.0 41.0 16-17 38.934897645557875 40.0 38.0 41.0 35.0 41.0 18-19 39.016334147806546 40.0 38.5 41.0 35.0 41.0 20-21 38.995936722095855 40.0 38.5 41.0 35.0 41.0 22-23 38.91979483670721 40.0 38.0 41.0 35.0 41.0 24-25 38.85805110235185 40.0 38.0 41.0 35.0 41.0 26-27 38.71478561004996 40.0 38.0 41.0 34.0 41.0 28-29 38.555141611012445 40.0 38.0 41.0 34.0 41.0 30-31 38.379245041320885 40.0 38.0 41.0 34.0 41.0 32-33 38.34267759584125 40.0 38.0 41.0 34.0 41.0 34-35 38.25766868510613 40.0 38.0 41.0 34.0 41.0 36-37 38.11647096805464 40.0 38.0 41.0 33.0 41.0 38-39 37.99594721676932 40.0 38.0 41.0 33.0 41.0 40-41 37.82267453182937 40.0 37.0 41.0 33.0 41.0 42-43 37.645710102064484 40.0 37.0 41.0 33.0 41.0 44-45 37.41953690576346 40.0 37.0 41.0 32.0 41.0 46-47 37.130338975789115 40.0 36.5 41.0 31.5 41.0 48-49 36.84072482922894 40.0 36.0 41.0 31.0 41.0 50-51 36.600913858854646 39.0 36.0 41.0 30.5 41.0 52-53 36.35204219854104 39.0 35.0 41.0 30.0 41.0 54-55 36.10191042006391 39.0 35.0 40.0 30.0 41.0 56-57 35.79461913207103 38.5 35.0 40.0 29.0 41.0 58-59 35.47938618926938 38.0 35.0 40.0 28.0 41.0 60-61 35.14361949819448 38.0 34.0 40.0 28.0 41.0 62-63 34.801916284097956 37.0 34.0 40.0 28.0 41.0 64-65 34.49661563009587 37.0 34.0 39.5 27.0 41.0 66-67 34.09344338242677 36.0 33.5 39.0 26.0 40.5 68-69 33.63785002440823 36.0 33.0 39.0 26.0 40.0 70-71 33.171211076662615 35.0 33.0 38.0 25.5 40.0 72-73 32.69648300771715 35.0 32.0 37.5 24.5 39.5 74-75 32.18978480942539 35.0 32.0 37.0 23.5 39.0 76-77 30.24307524659237 33.5 29.5 35.0 20.0 37.0 78-79 31.205088423574544 34.0 31.0 36.0 21.0 37.5 80-81 31.22897689700419 34.0 31.0 35.5 22.0 37.0 82-83 30.89900921627077 34.0 31.0 35.0 20.0 37.0 84-85 30.49218190103761 34.0 31.0 35.0 19.0 36.0 86-87 29.9222100181071 34.0 30.0 35.0 14.5 36.0 88-89 29.372750136755332 34.0 29.0 35.0 7.0 35.5 90-91 28.92776094302323 34.0 29.0 35.0 4.5 35.0 92-93 28.590834969204085 34.0 29.0 35.0 2.0 35.0 94-95 28.108162648829616 33.0 28.5 35.0 2.0 35.0 96-97 27.4194103205268 33.0 27.0 35.0 2.0 35.0 98-99 26.47924997489934 33.0 25.5 34.0 2.0 35.0 100-101 22.232544545020204 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25210920971437645 1101 2 0.2873148453215464 1101 3 0.2527577405525392 1101 4 0.1899610289162439 1101 5 0.2493306688828838 1101 6 0.22340357012104306 1101 7 0.21583669491035806 1101 8 0.23774387152307952 1101 9 0.2712626991201077 1101 10-11 0.31467294829266734 1101 12-13 0.329936097118555 1101 14-15 0.3662810178620859 1101 16-17 0.32744135170143807 1101 18-19 0.32747876259828246 1101 20-21 0.33208610304135533 1101 22-23 0.3239659483819395 1101 24-25 0.3028002688783431 1101 26-27 0.31766058119043095 1101 28-29 0.3146112198726172 1101 30-31 0.3558380882473102 1101 32-33 0.4195922683634592 1101 34-35 0.4818230399232917 1101 36-37 0.4436675110623014 1101 38-39 0.4658746625210952 1101 40-41 0.48745927596325345 1101 42-43 0.5384289726431959 1101 44-45 0.5699542669969802 1101 46-47 0.5424464159323819 1101 48-49 0.5421973388355426 1101 50-51 0.5776373832548884 1101 52-53 0.5422152705089829 1101 54-55 0.5704762561344481 1101 56-57 0.5380244989728808 1101 58-59 0.42060223978393196 1101 60-61 0.44900132234270274 1101 62-63 0.5039472381336836 1101 64-65 0.5233884949007077 1101 66-67 0.3704138893828528 1101 68-69 0.5539699812036787 1101 70-71 0.4389317192633442 1101 72-73 0.4346116821956727 1101 74-75 0.38733938188866546 1101 76-77 0.5547291240076966 1101 78-79 0.5963413702853053 1101 80-81 0.7195584768605237 1101 82-83 0.6308556415678659 1101 84-85 0.6400329507893616 1101 86-87 0.5648431333334827 1101 88-89 0.61678076888332 1101 90-91 0.8239706706584151 1101 92-93 0.8194124615732647 1101 94-95 0.7249063551930988 1101 96-97 0.6208833210593347 1101 98-99 0.5368016660035373 1101 100-101 0.43149674420388706 1102 1 0.21248362356453754 1102 2 0.22939921789107842 1102 3 0.2434855875439652 1102 4 0.17844261286005292 1102 5 0.18908247624270302 1102 6 0.21714408461946277 1102 7 0.18261380083784218 1102 8 0.20344460725760882 1102 9 0.28123077075846226 1102 10-11 0.29394659009949464 1102 12-13 0.2785842476735354 1102 14-15 0.3995884949301214 1102 16-17 0.37248867348059633 1102 18-19 0.3634754459821252 1102 20-21 0.38758251926671505 1102 22-23 0.3328840390342833 1102 24-25 0.4056098684294298 1102 26-27 0.3780758768217538 1102 28-29 0.3472547548091782 1102 30-31 0.3882761827780854 1102 32-33 0.4614483012284225 1102 34-35 0.42815409776774516 1102 36-37 0.5166603153186813 1102 38-39 0.4941192799824634 1102 40-41 0.4921601650474372 1102 42-43 0.5351353073594325 1102 44-45 0.644800074946815 1102 46-47 0.598431677173501 1102 48-49 0.5774822012987855 1102 50-51 0.5452046181404882 1102 52-53 0.5069380583370133 1102 54-55 0.36078993870616216 1102 56-57 0.3512428957056386 1102 58-59 0.4052403689731108 1102 60-61 0.4335269320795163 1102 62-63 0.3993095628044827 1102 64-65 0.43548535893457796 1102 66-67 0.35861610875813454 1102 68-69 0.35499742391588995 1102 70-71 0.3210004164720317 1102 72-73 0.32861070777206436 1102 74-75 0.3132012688596504 1102 76-77 0.4452026546875949 1102 78-79 0.4919332036939359 1102 80-81 0.5783742427182901 1102 82-83 0.6139561481718694 1102 84-85 0.43262547461528555 1102 86-87 0.37306391283111395 1102 88-89 0.5171390775809925 1102 90-91 0.6168237341788689 1102 92-93 0.6045217971291379 1102 94-95 0.5900658457146442 1102 96-97 0.44072815939922805 1102 98-99 0.3581992555944673 1102 100-101 0.35422347304936963 1103 1 0.19714400137151245 1103 2 0.2128407046698726 1103 3 0.1737305202918762 1103 4 0.10736853765407517 1103 5 0.1285526545289315 1103 6 0.164973148976145 1103 7 0.21207579545676936 1103 8 0.1775709187101029 1103 9 0.22519076020911655 1103 10-11 0.1996306450783436 1103 12-13 0.23814017902128626 1103 14-15 0.325700053143386 1103 16-17 0.20780080172081483 1103 18-19 0.22038659829454588 1103 20-21 0.23941037474971694 1103 22-23 0.3065600507019468 1103 24-25 0.2568182327486497 1103 26-27 0.21070924060331464 1103 28-29 0.30701839057599045 1103 30-31 0.36451723083081333 1103 32-33 0.3482339423446206 1103 34-35 0.33126151434358064 1103 36-37 0.4170439459466522 1103 38-39 0.48145307400080384 1103 40-41 0.4710149496630507 1103 42-43 0.4019970860110291 1103 44-45 0.4295335737645374 1103 46-47 0.5360537671893084 1103 48-49 0.41582443705858196 1103 50-51 0.4493288242511895 1103 52-53 0.23684118431682322 1103 54-55 0.24271775830779774 1103 56-57 0.45000164956967836 1103 58-59 0.41043962938691436 1103 60-61 0.5318595806108135 1103 62-63 0.448582286064088 1103 64-65 0.4882106729530946 1103 66-67 0.4255053643839801 1103 68-69 0.34303817699439065 1103 70-71 0.36683316970447066 1103 72-73 0.542473845840604 1103 74-75 0.36338738220627675 1103 76-77 0.45083758502455495 1103 78-79 0.42333038661078604 1103 80-81 0.4481355071458246 1103 82-83 0.5107665270541624 1103 84-85 0.3581818338228189 1103 86-87 0.264495058533214 1103 88-89 0.419784960222902 1103 90-91 0.5521420595945337 1103 92-93 0.42320851661045467 1103 94-95 0.17146693370095534 1103 96-97 0.07279016624272217 1103 98-99 -0.20055548566921644 1103 100-101 -0.22038593511188154 1104 1 0.1061093829748998 1104 2 0.14144588438655603 1104 3 0.13717076976623588 1104 4 0.1323287550213621 1104 5 0.12960775853672146 1104 6 0.12642185658116034 1104 7 0.13776269146936215 1104 8 0.17613474983065203 1104 9 0.23797436554997375 1104 10-11 0.18695587212926768 1104 12-13 0.20272309974552627 1104 14-15 0.21208093676545303 1104 16-17 0.23680230653315704 1104 18-19 0.23761236118867402 1104 20-21 0.23047661541556153 1104 22-23 0.24727724817558538 1104 24-25 0.2918821783208827 1104 26-27 0.28509404560712426 1104 28-29 0.33101647010158786 1104 30-31 0.27857393550939236 1104 32-33 0.24359510970217002 1104 34-35 0.33792590758577745 1104 36-37 0.4047695472862429 1104 38-39 0.3851590497006896 1104 40-41 0.4106471011370232 1104 42-43 0.43366174782216405 1104 44-45 0.3334165845902035 1104 46-47 0.34230616192888164 1104 48-49 0.369095014327506 1104 50-51 0.47449783674115764 1104 52-53 0.46890035321857937 1104 54-55 0.556795480226306 1104 56-57 0.4782607747842249 1104 58-59 0.3756416359135528 1104 60-61 0.4959988530176602 1104 62-63 0.580029889093332 1104 64-65 0.5223882584385819 1104 66-67 0.5295666498843161 1104 68-69 0.502850707621576 1104 70-71 0.4983020929744626 1104 72-73 0.41737041925922114 1104 74-75 0.4863950897212206 1104 76-77 0.5886938485890063 1104 78-79 0.6635058736777459 1104 80-81 0.7177986776419161 1104 82-83 0.7101680728542554 1104 84-85 0.5869706045015946 1104 86-87 0.6789181187700564 1104 88-89 0.7808261867923285 1104 90-91 0.8414944999770526 1104 92-93 0.7602139538227597 1104 94-95 0.7382269154437751 1104 96-97 0.5878343781967637 1104 98-99 0.593986257241184 1104 100-101 0.3191556191116298 1105 1 0.1526839499627215 1105 2 0.15774390632238777 1105 3 0.13400354496413058 1105 4 0.0970290173517796 1105 5 0.1438881905271785 1105 6 0.09787442599109397 1105 7 0.1627182967466041 1105 8 0.10658787285433391 1105 9 0.13662401476199904 1105 10-11 0.18683457651191304 1105 12-13 0.15301273155419182 1105 14-15 0.12829700974911162 1105 16-17 0.20597375447726307 1105 18-19 0.1811399401244671 1105 20-21 0.16733788731324495 1105 22-23 0.14846346399939137 1105 24-25 0.2187832757446202 1105 26-27 0.2250037226478625 1105 28-29 0.2091866779066649 1105 30-31 0.24404711827320114 1105 32-33 0.23400480125624057 1105 34-35 0.32377843982472854 1105 36-37 0.22571872539715088 1105 38-39 0.3313064032515811 1105 40-41 0.39923496149233983 1105 42-43 0.3418650402250947 1105 44-45 0.2619646947518106 1105 46-47 0.25126535913680925 1105 48-49 0.3389097636533265 1105 50-51 0.3633413213543122 1105 52-53 0.4605894567209532 1105 54-55 0.4656953334546543 1105 56-57 0.37568985556783474 1105 58-59 0.31859524030040376 1105 60-61 0.30895492651442424 1105 62-63 0.42288405477992796 1105 64-65 0.34531290886356203 1105 66-67 0.3188653104593513 1105 68-69 0.35034574108626515 1105 70-71 0.2710202616461217 1105 72-73 0.23760283377847458 1105 74-75 0.31879425803101924 1105 76-77 0.4632205520364714 1105 78-79 0.34759690381284614 1105 80-81 0.5020928733265926 1105 82-83 0.4886136335637232 1105 84-85 0.5910807743212523 1105 86-87 0.6361890574622215 1105 88-89 0.6503547469451298 1105 90-91 0.6724551365069509 1105 92-93 0.6588750609269738 1105 94-95 0.9007689428606405 1105 96-97 0.8257491334806808 1105 98-99 0.8756347861084102 1105 100-101 0.5203779101748616 1106 1 0.10046852611254309 1106 2 0.10962542821437182 1106 3 0.15093071343569164 1106 4 0.07627811668260875 1106 5 0.07509868345631787 1106 6 0.09608681874018998 1106 7 0.12492233133406927 1106 8 0.11166380026644163 1106 9 0.17770694231553819 1106 10-11 0.17808714090671174 1106 12-13 0.19877423987000498 1106 14-15 0.2398011559356661 1106 16-17 0.178411250843304 1106 18-19 0.15994199932570297 1106 20-21 0.25238427929500773 1106 22-23 0.27771195473598453 1106 24-25 0.31134414673652344 1106 26-27 0.2777432416994756 1106 28-29 0.2446094527302165 1106 30-31 0.27123289704547915 1106 32-33 0.2946858715210112 1106 34-35 0.3793781254480848 1106 36-37 0.32963101057092814 1106 38-39 0.36837444469070846 1106 40-41 0.37486144135712607 1106 42-43 0.34855034731168644 1106 44-45 0.5024950438877411 1106 46-47 0.42027512199428685 1106 48-49 0.35875501574312096 1106 50-51 0.3897047183040172 1106 52-53 0.49021441580431 1106 54-55 0.3932012227355699 1106 56-57 0.4578021191742536 1106 58-59 0.5283195551590936 1106 60-61 0.6703234718695867 1106 62-63 0.6141971114298954 1106 64-65 0.6105481602109677 1106 66-67 0.4872616002677077 1106 68-69 0.6724665722911922 1106 70-71 0.5512404359515344 1106 72-73 0.6418618818646635 1106 74-75 0.615056850650511 1106 76-77 0.6074952344559073 1106 78-79 0.7255259773352556 1106 80-81 0.7306635661241181 1106 82-83 0.8326980020269943 1106 84-85 0.7512245011431169 1106 86-87 0.7464003181254455 1106 88-89 0.9638210663341802 1106 90-91 1.0335619611921807 1106 92-93 1.0442308954142874 1106 94-95 1.2392556526714422 1106 96-97 1.150821898214648 1106 98-99 1.1706682950052532 1106 100-101 1.0819397068335697 1107 1 0.06402050094850154 1107 2 0.07537051499182468 1107 3 0.07806706135219343 1107 4 0.0648975143743229 1107 5 0.09197586842077499 1107 6 0.06713352358950431 1107 7 0.026681843573705066 1107 8 0.07359241217228174 1107 9 0.0707543940498283 1107 10-11 0.11042858313086867 1107 12-13 0.12455379147829859 1107 14-15 0.10783591675993165 1107 16-17 0.15595476226090454 1107 18-19 0.10045000380087288 1107 20-21 0.12639277798719917 1107 22-23 0.15708063755754864 1107 24-25 0.15851549242541552 1107 26-27 0.11042998403106452 1107 28-29 0.11241148296908676 1107 30-31 0.1388405378168116 1107 32-33 0.11765160683080467 1107 34-35 0.1857503042127746 1107 36-37 0.1120842746653139 1107 38-39 0.08525251150087598 1107 40-41 0.15752836133078318 1107 42-43 0.09902242232531222 1107 44-45 0.11458779411912445 1107 46-47 0.10272756175464082 1107 48-49 0.07848508660516984 1107 50-51 0.1386909791478459 1107 52-53 0.16287156262702496 1107 54-55 0.2119438756188643 1107 56-57 0.14459592370969432 1107 58-59 0.19280525123825498 1107 60-61 0.1669730332855366 1107 62-63 0.14251193111545035 1107 64-65 0.09759355916180112 1107 66-67 0.24702046914337927 1107 68-69 0.18739717870197836 1107 70-71 0.1824928178934826 1107 72-73 0.2111859778002767 1107 74-75 0.09029979914382125 1107 76-77 0.1158368239099481 1107 78-79 0.0960653716836184 1107 80-81 0.13696506303804057 1107 82-83 0.3045977967486486 1107 84-85 0.37913746757239863 1107 86-87 0.41040952996242197 1107 88-89 0.5432673132371768 1107 90-91 0.5794091196935902 1107 92-93 0.5210689302632971 1107 94-95 0.7947317995117906 1107 96-97 0.748963370038144 1107 98-99 0.6836981404982154 1107 100-101 0.623793350351658 1108 1 0.04268760973867103 1108 2 0.019456866157682384 1108 3 0.04745596763374493 1108 4 0.024001954936807124 1108 5 0.07420732007401654 1108 6 -0.03354346258549157 1108 7 0.03716683947047983 1108 8 0.0020797384798001417 1108 9 0.07960013678952294 1108 10-11 0.029632983124201928 1108 12-13 0.02320171791289738 1108 14-15 0.012643083606597827 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-0.4615217832429934 2316 74-75 -0.5507314897200821 2316 76-77 -0.6533417231941954 2316 78-79 -0.6633061792596564 2316 80-81 -0.6663441472236222 2316 82-83 -0.6627747335128866 2316 84-85 -0.6829837960409932 2316 86-87 -0.7275263063505548 2316 88-89 -1.0187139074027343 2316 90-91 -1.1574829427577527 2316 92-93 -0.896389964648332 2316 94-95 -0.7185936294562723 2316 96-97 -0.5728185661175154 2316 98-99 -0.48452581790495586 2316 100-101 -0.0577633387511689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 37.0 5 98.0 6 198.0 7 444.0 8 1190.0 9 2074.0 10 3515.0 11 5560.0 12 8295.0 13 11540.0 14 14239.0 15 18710.0 16 24429.0 17 33806.0 18 32428.0 19 22445.0 20 21866.0 21 23700.0 22 25267.0 23 27995.0 24 32613.0 25 39311.0 26 47385.0 27 57444.0 28 70640.0 29 86483.0 30 107258.0 31 131711.0 32 164300.0 33 212751.0 34 293459.0 35 429150.0 36 686183.0 37 1107048.0 38 826759.0 39 51045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3293024293503 19.010060042528494 14.002132067519524 23.658505460601674 2 15.887572402427667 20.509989198059806 39.24194268739809 24.360495712114442 3 19.368406748442894 24.272405370503087 29.44984974224216 26.909338138811854 4 13.14872228973435 16.21788413534277 36.18792779318439 34.44546578173849 5 14.166489230950328 37.459492724270085 33.456716071694416 14.917301973085165 6 33.07683053071456 35.416731582172645 17.00729996503218 14.499137922080621 7 29.837719890457247 32.38779571176823 20.049262213636066 17.72522218413846 8 26.8982808642937 35.052793617161235 19.495922440684538 18.553003077860524 9 27.19682727628386 16.591992196290644 19.79594027081018 36.41524025661532 10-11 25.64661253579008 26.28773971132507 26.84154471206944 21.224103040815407 12-13 26.07423261216534 23.43502780114736 27.74054873509973 22.750190851587572 14-15 22.882716517555505 24.963665751093004 25.771528684879254 26.38208904647224 16-17 22.876580569984345 28.43660571412497 26.175818564035875 22.51099515185481 18-19 22.643240515619983 26.75783768859053 28.522080379314612 22.076841416474878 20-21 23.328414901830445 26.157584121840344 28.246110695651872 22.26789028067734 22-23 23.007570013537045 26.04209943670652 28.269512735556734 22.680817814199706 24-25 22.937277339814496 26.28717711027327 28.34930471165398 22.426240838258256 26-27 22.699174631531356 26.5081797350819 28.342627613918363 22.45001801946838 28-29 22.457638304649336 26.60327234737932 28.61853095344433 22.32055839452702 30-31 22.643383205752727 26.20109914934721 28.396379272738603 22.759138372161463 32-33 22.463166941908412 26.51618819611061 28.375454841311882 22.645190020669098 34-35 23.125393286241408 26.443896067700194 27.933396028317475 22.497314617740923 36-37 23.108956419098657 26.177268189221593 28.01932644186308 22.69444894981667 38-39 22.73764224821286 26.430742827825064 28.268469803655027 22.56314512030705 40-41 22.81059693361688 26.232458045644652 28.351509361389283 22.60543565934919 42-43 22.898130309358926 26.308843495757056 27.802023183770125 22.99100301111389 44-45 22.73915387519138 26.534027634195667 27.828046392176987 22.898772098435963 46-47 22.830817095228852 26.800602763285365 27.391324198838312 22.97725594264747 48-49 23.217230106357935 26.686467407109916 27.260885069728154 22.835417416804 50-51 22.937358037872148 26.761314937429013 27.219174919412794 23.08215210528605 52-53 23.191093676243113 26.77318655499898 27.103541672143916 22.932178096613995 54-55 22.71467125056693 26.569418911012093 27.271837143440763 23.444072694980214 56-57 22.855827224549703 27.01537926896269 27.14722383129955 22.98156967518806 58-59 23.063680045770685 26.44485094395006 27.27886978086354 23.212599229415716 60-61 23.07332856706375 26.549358587637762 27.591020746255868 22.786292099042623 62-63 22.82287019410485 26.705443277575725 27.44994555153616 23.02174097678326 64-65 22.59207378907876 26.495846432300596 27.408483402769534 23.50359637585111 66-67 22.634954569748796 26.855542836864394 27.14610602483679 23.36339656855002 68-69 22.450206824203324 26.568178847756784 27.567461303230505 23.414153024809384 70-71 22.526269688065778 26.685623441080836 27.3437586215925 23.444348249260884 72-73 22.685167153100192 26.68680778432126 27.17361998289693 23.45440507968162 74-75 22.546460854152013 26.961333390891767 26.86202345376354 23.630182301192683 76-77 22.577885693669238 26.711406877020217 27.132680934908187 23.57802649440236 78-79 22.529097318814177 26.697877347671305 27.141196540952635 23.631828792561887 80-81 22.431459459482852 27.13481812502691 27.00141667364509 23.43230574184515 82-83 22.718306518901667 26.88604429141696 26.78801105814006 23.60763813154132 84-85 22.44008947953344 26.604473284239898 26.804467138905334 24.150970097321327 86-87 22.51864452747268 26.542901259171714 27.092478329283974 23.845975884071635 88-89 22.401110944468957 26.639752450608007 26.89626793857225 24.062868666350784 90-91 22.330519480378577 26.714583827437465 27.115082123090133 23.83981456909382 92-93 22.448431122051538 26.506331858453038 26.95242039627887 24.092816623216553 94-95 22.277660064326938 26.30349254077559 27.050659195324698 24.368188199572767 96-97 22.304697372566533 26.269877127930286 27.005337352901464 24.420088146601717 98-99 22.06669660715679 26.436756737340577 26.75221380928831 24.744332846214327 100-101 22.12762799135712 25.96690618044213 27.405957943482857 24.499507884717897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 689.0 1 495.0 2 425.5 3 591.5 4 519.5 5 503.0 6 945.5 7 1913.0 8 2972.0 9 3426.5 10 3171.0 11 2974.5 12 3260.0 13 3659.5 14 4037.0 15 4428.5 16 4714.5 17 4968.0 18 5337.0 19 5685.0 20 6253.0 21 7113.0 22 8403.0 23 10479.5 24 12846.5 25 15597.0 26 19494.0 27 24694.5 28 29814.0 29 35300.0 30 42659.0 31 51206.5 32 61381.0 33 69346.0 34 76625.0 35 86338.5 36 97707.5 37 107875.5 38 114230.0 39 118494.0 40 122772.5 41 134799.0 42 148650.5 43 157947.0 44 165968.0 45 173867.5 46 174539.0 47 176108.0 48 192827.0 49 196620.0 50 185043.5 51 177718.0 52 171707.0 53 171621.5 54 172386.0 55 159275.0 56 139212.5 57 125556.0 58 114470.5 59 99528.5 60 84381.0 61 70146.5 62 57328.0 63 45633.0 64 35472.0 65 27545.5 66 20742.0 67 17918.5 68 13708.0 69 8312.5 70 6293.5 71 5244.0 72 4463.0 73 3782.5 74 3041.0 75 2551.0 76 1722.5 77 939.0 78 511.0 79 285.0 80 182.0 81 121.5 82 80.5 83 52.5 84 30.0 85 16.5 86 11.5 87 12.0 88 8.5 89 4.0 90 4.0 91 1.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29553866021319986 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013740448765220523 16-17 8.006245737215108E-4 18-19 8.655400796989305E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.2457752988709895E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001644526151427968 36-37 0.007908872478248978 38-39 0.0024343314741532424 40-41 0.003234956047874753 42-43 2.704812749059158E-4 44-45 0.0011360213546048464 46-47 1.9474651793225937E-4 48-49 3.462160318795722E-4 50-51 2.1638501992473264E-5 52-53 3.2457752988709895E-5 54-55 0.0 56-57 8.871785816914037E-4 58-59 0.00202319993629625 60-61 6.058780557892514E-4 62-63 0.0010711058486274265 64-65 7.573475697365642E-5 66-67 4.7604704383441185E-4 68-69 0.001644526151427968 70-71 2.1638501992473264E-5 72-73 0.0 74-75 3.2457752988709895E-5 76-77 0.2605492024913706 78-79 8.655400796989305E-5 80-81 6.491550597741979E-5 82-83 0.0 84-85 0.0 86-87 0.10871183401018568 88-89 0.37610962238217405 90-91 0.27876882116903307 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.409625498118316E-5 100-101 3.353967808833356E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4621392.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.93034155410944 #Duplication Level Percentage of deduplicated Percentage of total 1 63.01465188879622 20.750940096113666 2 15.761809742073495 10.380835566347395 3 7.056390086969848 6.971080071088473 4 3.9482710991976444 5.2007166337919015 5 2.3926100281121427 3.939473271576012 6 1.6001863905341474 3.1616810634316215 7 1.1516889088819278 2.654785639349305 8 0.8278193184020477 2.1808298320055606 9 0.643002213504585 1.9056854259678946 >10 3.1440895669966133 18.519076480742292 >50 0.25370414947926123 5.839091180773934 >100 0.1859526545536957 11.900715287778642 >500 0.01448926781598631 3.1928675489558156 >1k 0.00513710376831272 2.795911522883867 >5k 1.3172060944391487E-4 0.34175987105925426 >10k+ 6.586030472195743E-5 0.26455050813433073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12198 0.26394644730418887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9212 0.1993338803546637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6546 0.14164563404272998 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2457752988709895E-5 0.0 12-13 0.0 0.0 0.0 5.409625498118316E-5 0.0 14-15 0.0 0.0 0.0 6.491550597741979E-5 0.0 16-17 0.0 0.0 0.0 7.573475697365642E-5 0.0 18-19 0.0 0.0 0.0 8.655400796989305E-5 0.0 20-21 0.0 0.0 0.0 1.0819250996236632E-4 0.0 22-23 0.0 0.0 0.0 2.9211977689838907E-4 0.0 24-25 0.0 0.0 0.0 6.599743107704346E-4 0.0 26-27 0.0 0.0 0.0 0.0010819250996236631 0.0 28-29 0.0 0.0 0.0 0.00298611327496131 0.0 30-31 0.0 0.0 0.0 0.01040811945837964 0.0 32-33 0.0 0.0 0.0 0.024083652717622743 0.0 34-35 0.0 0.0 0.0 0.0443913868375589 0.0 36-37 0.0 0.0 0.0 0.08037621565104194 0.0 38-39 0.0 0.0 0.0 0.13849723200282513 0.0 40-41 0.0 0.0 0.0 0.2042999165619363 0.0 42-43 0.0 0.0 0.0 0.2717904042764604 0.0 44-45 0.0 0.0 0.0 0.3521990776804911 0.0 46-47 0.0 0.0 0.0 0.45295876220844283 0.0 48-49 0.0 0.0 0.0 0.5633692186250376 0.0 50-51 0.0 0.0 0.0 0.688526314149503 0.0 52-53 0.0 0.0 0.0 0.814624684510641 0.0 54-55 0.0 0.0 0.0 0.9523970266967182 0.0 56-57 0.0 0.0 0.0 1.1166007990666016 0.0 58-59 0.0 0.0 0.0 1.3044662733652546 2.1638501992473264E-5 60-61 0.0 0.0 0.0 1.5147167779751207 2.1638501992473264E-5 62-63 0.0 0.0 0.0 1.732410927270398 2.1638501992473264E-5 64-65 0.0 0.0 0.0 1.9580139490439246 2.1638501992473264E-5 66-67 0.0 0.0 0.0 2.2150036179575334 2.1638501992473264E-5 68-69 2.1638501992473264E-5 0.0 0.0 2.5059549157483287 2.1638501992473264E-5 70-71 2.1638501992473264E-5 0.0 0.0 2.8342001717231518 2.1638501992473264E-5 72-73 2.1638501992473264E-5 0.0 0.0 3.1797995062959385 2.1638501992473264E-5 74-75 2.1638501992473264E-5 0.0 0.0 3.5300078417931218 2.1638501992473264E-5 76-77 2.1638501992473264E-5 0.0 0.0 3.916622956892642 2.1638501992473264E-5 78-79 2.1638501992473264E-5 0.0 0.0 4.344124021506939 2.1638501992473264E-5 80-81 2.1638501992473264E-5 0.0 0.0 4.823059805357347 2.1638501992473264E-5 82-83 2.1638501992473264E-5 0.0 0.0 5.312868936458972 2.1638501992473264E-5 84-85 2.1638501992473264E-5 0.0 0.0 5.813183560277942 2.1638501992473264E-5 86-87 2.1638501992473264E-5 0.0 0.0 6.3443114109341945 2.1638501992473264E-5 88-89 2.1638501992473264E-5 0.0 0.0 6.923941963806576 2.1638501992473264E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19410 0.0 32.515896 1 GGTATCA 13130 0.0 30.849104 1 GTACATG 20890 0.0 27.93378 1 TACATGG 21270 0.0 26.816769 2 ATCAACG 24360 0.0 25.674831 3 TCAACGC 24400 0.0 25.65219 4 ACATGGG 21380 0.0 25.56903 3 TATCAAC 24670 0.0 25.448383 2 CAACGCA 24645 0.0 25.320158 5 AACGCAG 25275 0.0 24.670256 6 CATGGGG 10045 0.0 22.817427 4 ACGCAGA 28880 0.0 21.426432 7 CGCAGAG 29105 0.0 21.162966 8 GAGTACT 13185 0.0 21.036745 12-13 CATGGGA 11965 0.0 20.742374 4 GCAGAGT 31170 0.0 19.487106 9 AGTACTT 14185 0.0 19.453356 12-13 GTACTTT 14070 0.0 19.426859 14-15 GCACCGT 1280 0.0 18.907307 6 AGAGTAC 25515 0.0 18.858908 10-11 >>END_MODULE