FastQCFastQC Report
Fri 27 May 2016
SRR939334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712922
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122000.25886276072466297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87390.1854263660633467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60250.12784001093164707No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG48850.10365119558524415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194150.033.760981
GGTATCA130700.030.0904061
GTACATG214750.027.7577251
ATCAACG238800.027.083823
TCAACGC240250.026.8608654
TACATGG219100.026.7921852
CAACGCA241500.026.6825665
TATCAAC244300.026.609942
AACGCAG246800.026.1510876
ACATGGG219550.025.8301933
ACGCAGA279300.023.040227
CGCAGAG280850.022.7612088
CATGGGG102850.022.4056344
GAGTACT122150.022.09434312-13
CATGGGA117500.020.9034124
GCAGAGT301950.020.7469469
GTACTTT131350.020.40282214-15
AGTACAT162550.019.7483842
AGTACTT132000.019.65581112-13
GAGTACA183600.019.5579091
Produced by FastQC (version 0.11.5)