FastQCFastQC Report
Fri 27 May 2016
SRR939334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712922
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127430.270384275402818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94910.2013824968883423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67850.14396588782924902No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG47190.10012896457866267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139900.031.1540781
GTATCAA201950.031.1004891
GTACATG220000.027.9216251
TACATGG224800.026.8929442
ACATGGG226450.025.5863653
ATCAACG252400.024.5726223
TCAACGC252600.024.5155934
TATCAAC254750.024.4204542
CAACGCA255300.024.256325
AACGCAG261000.023.781136
CATGGGA122450.021.1591914
CATGGGG104450.020.762184
GTATCGC1150.003746313320.6318136
ACGCAGA299200.020.5863027
GAGTACT133750.020.57935112-13
CCCTATA7650.020.4699942
CGCAGAG301600.020.3438178
GTACTTT138900.019.54300114-15
AGTACAT164200.019.3338432
CCTATAC9650.019.1779653
Produced by FastQC (version 0.11.5)