##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939334_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4712922 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3015895446604 33.0 31.0 34.0 31.0 34.0 2 32.50399603473174 34.0 31.0 34.0 31.0 34.0 3 32.59753439585887 34.0 31.0 34.0 31.0 34.0 4 36.12730637171589 37.0 35.0 37.0 35.0 37.0 5 36.06186331961361 37.0 35.0 37.0 35.0 37.0 6 36.095426149637106 37.0 35.0 37.0 35.0 37.0 7 36.03754952872125 37.0 35.0 37.0 35.0 37.0 8 36.01332336923887 37.0 35.0 37.0 35.0 37.0 9 37.724884901553644 39.0 37.0 39.0 35.0 39.0 10-11 37.64678579446043 39.0 37.0 39.0 35.0 39.0 12-13 37.57144421231669 39.0 37.0 39.0 35.0 39.0 14-15 38.89301615855302 40.0 38.0 41.0 35.0 41.0 16-17 38.844620916705175 40.0 38.0 41.0 35.0 41.0 18-19 38.93738247736755 40.0 38.0 41.0 35.0 41.0 20-21 38.93771422484819 40.0 38.0 41.0 35.0 41.0 22-23 38.88111260912021 40.0 38.0 41.0 35.0 41.0 24-25 38.815984117708716 40.0 38.0 41.0 34.5 41.0 26-27 38.66266331163554 40.0 38.0 41.0 34.0 41.0 28-29 38.52131331687645 40.0 38.0 41.0 34.0 41.0 30-31 38.35037159537119 40.0 38.0 41.0 34.0 41.0 32-33 38.174852034470334 40.0 38.0 41.0 33.5 41.0 34-35 38.1358329291255 40.0 38.0 41.0 33.0 41.0 36-37 38.06072623310973 40.0 38.0 41.0 33.0 41.0 38-39 37.96206249541155 40.0 37.5 41.0 33.0 41.0 40-41 37.79952522023492 40.0 37.0 41.0 33.0 41.0 42-43 37.63307964782783 40.0 37.0 41.0 33.0 41.0 44-45 37.42393975542137 40.0 37.0 41.0 32.0 41.0 46-47 37.15836661417269 40.0 37.0 41.0 31.5 41.0 48-49 36.86979595673343 40.0 36.0 41.0 31.0 41.0 50-51 36.64189031772646 39.0 36.0 41.0 31.0 41.0 52-53 36.41968146300745 39.0 35.0 41.0 30.0 41.0 54-55 36.14378245597954 39.0 35.0 40.5 30.0 41.0 56-57 35.9060567944897 39.0 35.0 40.0 29.0 41.0 58-59 35.6092034835289 38.0 35.0 40.0 28.5 41.0 60-61 35.283096346597716 38.0 34.5 40.0 28.0 41.0 62-63 34.9101068296908 37.5 34.0 40.0 28.0 41.0 64-65 34.492889867475 37.0 34.0 39.5 27.0 41.0 66-67 34.12379665948217 36.0 33.5 39.0 26.0 40.5 68-69 33.74365223952359 36.0 33.0 39.0 26.0 40.0 70-71 33.362860026115435 35.0 33.0 38.5 26.0 40.0 72-73 32.88904399436273 35.0 32.5 37.5 25.5 39.5 74-75 32.39913348873586 35.0 32.0 37.0 24.5 39.0 76-77 30.409306476958456 33.5 29.5 35.0 20.5 37.5 78-79 31.395960828547555 34.0 31.0 36.0 22.0 38.0 80-81 31.451994007114905 35.0 31.0 35.5 23.0 37.0 82-83 31.167233194183993 34.0 31.0 35.0 21.5 37.0 84-85 30.823360115019938 34.0 31.0 35.0 20.0 36.5 86-87 30.313747501019535 34.0 30.5 35.0 18.5 36.0 88-89 29.843418796237238 34.0 30.0 35.0 13.5 36.0 90-91 29.460017903967007 34.0 30.0 35.0 7.5 35.0 92-93 29.138311646150733 34.0 29.0 35.0 3.5 35.0 94-95 28.772965794893274 34.0 29.0 35.0 2.0 35.0 96-97 28.258456643245953 34.0 29.0 35.0 2.0 35.0 98-99 27.549819729670894 33.0 28.5 35.0 2.0 35.0 100-101 24.265217523226568 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2061331647994109 1101 2 0.17299793280257347 1101 3 0.24228982212160588 1101 4 0.1257726184048522 1101 5 0.14511188922148932 1101 6 0.06664683407127825 1101 7 0.1651929308570672 1101 8 0.22019798426480008 1101 9 0.272567924696304 1101 10-11 0.23106956703819037 1101 12-13 0.2502299163544066 1101 14-15 0.34454389666633034 1101 16-17 0.3394499129117392 1101 18-19 0.2307606530302877 1101 20-21 0.21358515450028648 1101 22-23 0.2637802154975475 1101 24-25 0.23229615091337763 1101 26-27 0.3026628736235537 1101 28-29 0.259441956801588 1101 30-31 0.2614505374601208 1101 32-33 0.190936855785381 1101 34-35 0.2420863642709321 1101 36-37 0.3102685259114466 1101 38-39 0.3701113032282777 1101 40-41 0.43860931983280693 1101 42-43 0.45091009140322313 1101 44-45 0.5368319631741514 1101 46-47 0.4364095905849297 1101 48-49 0.4781394775049961 1101 50-51 0.48333783674118536 1101 52-53 0.48894830356091035 1101 54-55 0.5020883043626156 1101 56-57 0.537229994840672 1101 58-59 0.37981633068419285 1101 60-61 0.4092911814559912 1101 62-63 0.46839478178589644 1101 64-65 0.3850238093286862 1101 66-67 0.35935917344767176 1101 68-69 0.3809438191750303 1101 70-71 0.3300757512307655 1101 72-73 0.2126313489313958 1101 74-75 0.20409609249846739 1101 76-77 0.23516163531122558 1101 78-79 0.5156063148646588 1101 80-81 0.5062208883070127 1101 82-83 0.45319685471895 1101 84-85 0.4637346657239725 1101 86-87 0.5509748259017151 1101 88-89 0.7188214647743081 1101 90-91 0.65497648013082 1101 92-93 0.6839671583776337 1101 94-95 0.7796091489238428 1101 96-97 0.5931254945524138 1101 98-99 0.49417750489641676 1101 100-101 0.4022465518464209 1102 1 0.1333463489506741 1102 2 0.1879797498034037 1102 3 0.17855948094084084 1102 4 0.1627447633772121 1102 5 0.11183183519744233 1102 6 0.11714839591377313 1102 7 0.15048257795653797 1102 8 0.16141391161455942 1102 9 0.2870541853259212 1102 10-11 0.29019722543914384 1102 12-13 0.24460756334635647 1102 14-15 0.24668128449069826 1102 16-17 0.29303744706523815 1102 18-19 0.23289638900009635 1102 20-21 0.23999780513052116 1102 22-23 0.17864167109500073 1102 24-25 0.2390400011847973 1102 26-27 0.28781048922746777 1102 28-29 0.26892125024459546 1102 30-31 0.3447354210589637 1102 32-33 0.3160382978544263 1102 34-35 0.29973250510468574 1102 36-37 0.34647023471301 1102 38-39 0.3364500347663508 1102 40-41 0.4296479527915622 1102 42-43 0.41963454890198904 1102 44-45 0.430939489947491 1102 46-47 0.46507917851440084 1102 48-49 0.3627311740520369 1102 50-51 0.3321098508530582 1102 52-53 0.3424231476356425 1102 54-55 0.4162576328949328 1102 56-57 0.3207406225650118 1102 58-59 0.322552748169997 1102 60-61 0.22004775044766234 1102 62-63 0.26254990436890324 1102 64-65 0.33195114984562935 1102 66-67 0.2806291248411483 1102 68-69 0.3145342538731626 1102 70-71 0.26369762716080913 1102 72-73 0.4096325609446865 1102 74-75 0.39787909984250547 1102 76-77 0.49691423061273454 1102 78-79 0.4349879165084509 1102 80-81 0.4784170961528744 1102 82-83 0.46923403880526493 1102 84-85 0.4165286657402092 1102 86-87 0.5430630024586485 1102 88-89 0.5937647067712817 1102 90-91 0.6532567595350187 1102 92-93 0.6600097515877259 1102 94-95 0.5630063586334977 1102 96-97 0.4287414669222045 1102 98-99 0.3325649532654644 1102 100-101 0.2254092206050622 1103 1 0.14861159746296693 1103 2 0.12962678095146174 1103 3 0.12454853121931109 1103 4 0.07143286889730405 1103 5 0.05757085895628222 1103 6 0.042436745359495376 1103 7 0.11209968726743114 1103 8 0.0737141281136715 1103 9 0.09686760255471683 1103 10-11 0.10958318471048756 1103 12-13 0.13841246791141515 1103 14-15 0.1943718781841426 1103 16-17 0.19311881973226974 1103 18-19 0.1242617074802368 1103 20-21 0.18936685779438278 1103 22-23 0.18775769076514592 1103 24-25 0.21247079484448506 1103 26-27 0.20558842456573956 1103 28-29 0.16332687251603062 1103 30-31 0.20584109999720823 1103 32-33 0.2112195024996737 1103 34-35 0.28261563680638346 1103 36-37 0.3823970229467548 1103 38-39 0.2988605069696533 1103 40-41 0.32920330241920936 1103 42-43 0.2739606012915843 1103 44-45 0.36195371601125714 1103 46-47 0.37247738573246636 1103 48-49 0.3253178575153868 1103 50-51 0.36692723915595593 1103 52-53 0.20083803226562935 1103 54-55 0.22286681929467278 1103 56-57 0.25551846501588216 1103 58-59 0.2137516113160629 1103 60-61 0.08885849482755503 1103 62-63 0.10981538702341709 1103 64-65 0.2752865882604496 1103 66-67 0.15906190541195997 1103 68-69 0.09960383973272968 1103 70-71 0.22292618349811733 1103 72-73 0.23727610084532103 1103 74-75 0.19145763944359118 1103 76-77 0.25131607692859603 1103 78-79 0.40844110757565133 1103 80-81 0.38324927498916495 1103 82-83 0.3107910406195913 1103 84-85 0.21261555009318656 1103 86-87 0.15109105775358955 1103 88-89 0.3701193926661901 1103 90-91 0.4520838499001769 1103 92-93 0.300971504510521 1103 94-95 0.2789624366303407 1103 96-97 0.08838235540950024 1103 98-99 0.05405374054615564 1103 100-101 0.018440136722240652 1104 1 0.13140373256178606 1104 2 0.09102581035376289 1104 3 0.1319339465318805 1104 4 0.07111544386534518 1104 5 0.11218446650907765 1104 6 0.12900935243627742 1104 7 0.15649665902304122 1104 8 0.13391511889711438 1104 9 0.09886435133029181 1104 10-11 0.1665046580268239 1104 12-13 0.18621120542960767 1104 14-15 0.24644289153494725 1104 16-17 0.17582921642146232 1104 18-19 0.11282083205787785 1104 20-21 0.17015431248426438 1104 22-23 0.17292854400341895 1104 24-25 0.14370305446888665 1104 26-27 0.1604296433799206 1104 28-29 0.21222105377397327 1104 30-31 0.2069745250819821 1104 32-33 0.2417659526465954 1104 34-35 0.19090527429241888 1104 36-37 0.2657845788421511 1104 38-39 0.3450503206444111 1104 40-41 0.3976963634111357 1104 42-43 0.39454048566859967 1104 44-45 0.4202308332277056 1104 46-47 0.38267099849990416 1104 48-49 0.36758763440438713 1104 50-51 0.28394633118301726 1104 52-53 0.369228125751377 1104 54-55 0.38042919520321306 1104 56-57 0.4647097322555993 1104 58-59 0.45419000067707316 1104 60-61 0.43166499310299145 1104 62-63 0.46971072246709866 1104 64-65 0.40723495194319526 1104 66-67 0.4264857875493817 1104 68-69 0.4448995666903457 1104 70-71 0.35183191406596137 1104 72-73 0.40970584440656665 1104 74-75 0.37403593451291073 1104 76-77 0.4071274782310219 1104 78-79 0.5023096196193286 1104 80-81 0.5527357171661151 1104 82-83 0.6377177967231411 1104 84-85 0.5292836966216505 1104 86-87 0.41746208210221525 1104 88-89 0.7062945663556555 1104 90-91 0.7409495720317452 1104 92-93 0.7140231296489645 1104 94-95 0.7587493876348823 1104 96-97 0.7591281440049613 1104 98-99 0.8048276869723914 1104 100-101 0.7648154068901754 1105 1 0.08493844502808656 1105 2 0.08397060715489602 1105 3 0.13919530576264094 1105 4 0.07364878334583835 1105 5 0.09588595874260619 1105 6 0.08975515721756722 1105 7 0.12451672384886336 1105 8 0.1262051094538208 1105 9 0.07047551956991072 1105 10-11 0.15794548207118453 1105 12-13 0.11381276374511629 1105 14-15 0.20304678983192304 1105 16-17 0.2091345688662969 1105 18-19 0.10328029479706657 1105 20-21 0.12325088556040242 1105 22-23 0.15775322939703074 1105 24-25 0.18802120457237237 1105 26-27 0.21553904150966474 1105 28-29 0.1641952046550088 1105 30-31 0.19875436812071 1105 32-33 0.15866847034575926 1105 34-35 0.23614752870294353 1105 36-37 0.2908822896569987 1105 38-39 0.31586150993650364 1105 40-41 0.34188582404249956 1105 42-43 0.27449882477774423 1105 44-45 0.38126027601997237 1105 46-47 0.37393082566909186 1105 48-49 0.4019919717093785 1105 50-51 0.45913033610650444 1105 52-53 0.41645785074766195 1105 54-55 0.42294246492371457 1105 56-57 0.38012732003265626 1105 58-59 0.379902489235306 1105 60-61 0.2716564569788815 1105 62-63 0.2204710609659486 1105 64-65 0.22475728435686904 1105 66-67 0.231778868082273 1105 68-69 0.3244837565015146 1105 70-71 0.2529019466217548 1105 72-73 0.14326220248525345 1105 74-75 0.13474226074818318 1105 76-77 0.33442193550175503 1105 78-79 0.2642740835825528 1105 80-81 0.2391189647758587 1105 82-83 0.32902273048260255 1105 84-85 0.26984108476093027 1105 86-87 0.26529004507348475 1105 88-89 0.43089567807685825 1105 90-91 0.5737725008587802 1105 92-93 0.587055736275861 1105 94-95 0.6250040814623397 1105 96-97 0.36278695356206825 1105 98-99 0.5410022677743243 1105 100-101 0.5680705443308689 1106 1 0.13502859266953493 1106 2 0.1006952734819535 1106 3 0.12942741948453573 1106 4 0.0649621720154272 1106 5 0.07886796175141342 1106 6 0.09461848524644267 1106 7 0.11339633731802934 1106 8 0.018801875663321255 1106 9 0.1448135572051541 1106 10-11 0.1503638654839179 1106 12-13 0.15187014478662064 1106 14-15 0.1894868161298433 1106 16-17 0.12109639961723673 1106 18-19 0.08463817484841485 1106 20-21 0.11725206236031482 1106 22-23 0.12621563774361277 1106 24-25 0.11844985232297489 1106 26-27 0.18918950312669125 1106 28-29 0.21423560281971277 1106 30-31 0.26738415625946743 1106 32-33 0.28134620923079723 1106 34-35 0.2736199373649626 1106 36-37 0.21296574210675345 1106 38-39 0.3799435799320108 1106 40-41 0.3316665413638873 1106 42-43 0.36841247429057233 1106 44-45 0.5170707839141926 1106 46-47 0.5617179376486376 1106 48-49 0.376555856569766 1106 50-51 0.46563460206824914 1106 52-53 0.5347629260635998 1106 54-55 0.6080118596069468 1106 56-57 0.6073852644876041 1106 58-59 0.6297929931952666 1106 60-61 0.49963538386553097 1106 62-63 0.6365362921420541 1106 64-65 0.615272470862763 1106 66-67 0.5846909759325811 1106 68-69 0.6167064702299072 1106 70-71 0.6066483454973124 1106 72-73 0.599810641908995 1106 74-75 0.6332651756850183 1106 76-77 0.7448166204792734 1106 78-79 0.9225126391538119 1106 80-81 0.8102486951674734 1106 82-83 0.7542038391930355 1106 84-85 0.8109768978519227 1106 86-87 0.7447942044824316 1106 88-89 0.9119043431292511 1106 90-91 0.912885897128632 1106 92-93 0.8647694857843895 1106 94-95 0.8700515496163383 1106 96-97 0.8864769440319265 1106 98-99 0.924585139151187 1106 100-101 0.9684960352374965 1107 1 -0.008517344066625299 1107 2 0.04122875258472902 1107 3 0.03100187676299271 1107 4 0.026264185824089736 1107 5 0.02197122120463746 1107 6 0.07534812592809459 1107 7 0.03301109357588672 1107 8 0.05645343870563835 1107 9 0.09140502052272836 1107 10-11 0.07747097157798777 1107 12-13 0.12472821176881865 1107 14-15 0.12231283093912992 1107 16-17 0.07612146621244165 1107 18-19 0.03836637802859144 1107 20-21 0.0973262869445577 1107 22-23 0.11602227704763379 1107 24-25 0.08312456624212672 1107 26-27 0.1229942827775048 1107 28-29 0.0445494466169194 1107 30-31 0.12789968975638288 1107 32-33 0.14177061306917693 1107 34-35 0.2623078151961096 1107 36-37 0.3222242873533574 1107 38-39 0.22280205314913104 1107 40-41 0.25429788887363003 1107 42-43 0.230177999769694 1107 44-45 0.36011256173124906 1107 46-47 0.37144424696635525 1107 48-49 0.3314538737869981 1107 50-51 0.2571340056631186 1107 52-53 0.25663851032325624 1107 54-55 0.20425423409044896 1107 56-57 0.24428686084831241 1107 58-59 0.2553001988762844 1107 60-61 0.23175542889821799 1107 62-63 0.18384748020815778 1107 64-65 0.2948756292323793 1107 66-67 0.3066554092336773 1107 68-69 0.3189543747347727 1107 70-71 0.15959066227645735 1107 72-73 0.22852653332026307 1107 74-75 0.24431115458883568 1107 76-77 0.2107377866728335 1107 78-79 0.4816203667160437 1107 80-81 0.3914160005257159 1107 82-83 0.44407215018463475 1107 84-85 0.4113297283234374 1107 86-87 0.4463626751409855 1107 88-89 0.6050198965340599 1107 90-91 0.7179036348657419 1107 92-93 0.725300983601052 1107 94-95 0.7676628882744403 1107 96-97 0.6654464234359345 1107 98-99 0.9204884618915301 1107 100-101 0.8743730192258994 1108 1 0.027975009053463396 1108 2 0.028001801154339034 1108 3 0.07074418301623098 1108 4 0.01984595621622276 1108 5 0.05713107798266748 1108 6 0.04021549470287056 1108 7 0.07354527447957082 1108 8 0.05540564385873381 1108 9 0.04498623084634801 1108 10-11 0.059122504726651925 1108 12-13 0.052315631074222324 1108 14-15 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#Quality Count 2 4.0 3 16.0 4 38.0 5 91.0 6 202.0 7 512.0 8 1172.0 9 2158.0 10 3595.0 11 5743.0 12 8046.0 13 12168.0 14 13984.0 15 18357.0 16 24622.0 17 33779.0 18 32103.0 19 22253.0 20 21342.0 21 23075.0 22 24097.0 23 27184.0 24 31749.0 25 38001.0 26 45418.0 27 56647.0 28 69097.0 29 85665.0 30 105218.0 31 129087.0 32 161492.0 33 207771.0 34 284543.0 35 411043.0 36 656380.0 37 1105991.0 38 966719.0 39 83554.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28809630650453 19.035062578844578 14.040202037868376 23.63663907678252 2 15.915158366720265 20.555379444005226 39.20627160814459 24.32319058112992 3 19.359030342534844 24.328580018935174 29.45051923201784 26.86187040651214 4 13.185599082692223 16.24340483462277 36.16244868894499 34.40854739374002 5 14.186103652893046 37.52105381756795 33.42236514841536 14.870477381123642 6 33.098616951436924 35.372301939221565 17.037858890938573 14.491222218402935 7 29.8558516351427 32.40779287244728 19.99625709909903 17.740098393310983 8 26.92476132641279 35.04957221867877 19.511462315735333 18.514204139173106 9 27.19088073174137 16.5845095675252 19.826256407383784 36.39835329334964 10-11 25.673043177884125 26.283248481515287 26.82346535758495 21.220242983015634 12-13 26.069951932155888 23.48067716800745 27.70238930328149 22.746981596555173 14-15 22.906036061727956 24.995501620265458 25.7289072671749 26.36955505083169 16-17 22.857925815056003 28.436681302392135 26.1780174699341 22.527375412617758 18-19 22.629665842125117 26.78345833009755 28.539195004712575 22.04768082306476 20-21 23.31916377992252 26.13942051237003 28.26924570362081 22.272170004086636 22-23 23.0072341532493 26.05549168859574 28.266487329940958 22.670786828214005 24-25 22.93072597652284 26.275159290809246 28.376202359428472 22.41791237323944 26-27 22.697927569870863 26.519301496136222 28.325397268290487 22.45737366570243 28-29 22.468916311366918 26.608450129240417 28.59686623288058 22.32576732651209 30-31 22.66520642607707 26.19964854075667 28.416309457275123 22.718835575891134 32-33 22.45927261261697 26.472589616378116 28.379453341260476 22.688684429744434 34-35 23.10113558781669 26.46588541846255 27.95302962225406 22.479949371466702 36-37 23.117753447868967 26.162865224529575 28.050735839670487 22.668645487930974 38-39 22.758057780565583 26.420238730820518 28.247949958706343 22.57375352990756 40-41 22.81879785716211 26.21663647115524 28.346666825125016 22.617898846557637 42-43 22.906432738610818 26.28190492276784 27.791164846114963 23.02049749250638 44-45 22.741254681572443 26.500909314087973 27.826931356175493 22.930904648164088 46-47 22.860528087797995 26.786022519634635 27.401862904433028 22.951586488134346 48-49 23.20630385989838 26.661697350391115 27.275902295858067 22.856096493852434 50-51 22.904686307135997 26.770971384631448 27.24190003568911 23.082442272543446 52-53 23.2109425119854 26.75858276018389 27.101894228452565 22.928580499378146 54-55 22.70540441789616 26.563785693885873 27.290808122858813 23.440001765359156 56-57 22.85375187622456 27.0068759890361 27.14745756454276 22.991914570196577 58-59 23.080298427688003 26.414847926452083 27.305339885703084 23.19951376015683 60-61 23.065804510997406 26.5507747136651 27.640733672576907 22.742687102760588 62-63 22.84307527390737 26.68005414544858 27.44036717395578 23.036503406688272 64-65 22.576985695283245 26.483331674772398 27.424679938407625 23.515002691536736 66-67 22.641197965932815 26.831326722572534 27.187326673346174 23.340148638148477 68-69 22.445249705223773 26.5592161254532 27.559344072234126 23.4361900970889 70-71 22.53731336949773 26.68276708165338 27.361655889912882 23.418263658936006 72-73 22.723429328981045 26.65606390260649 27.172643638065725 23.447863130346736 74-75 22.553449855524875 26.93963532602492 26.896912361375808 23.6100024570744 76-77 22.60559821656062 26.703163767358312 27.13514632089063 23.55609169519044 78-79 22.509114303186006 26.71512492674396 27.168877397079772 23.606883372990257 80-81 22.448031904143285 27.136684831474682 27.002286824316617 23.412996440065417 82-83 22.738038100354725 26.87704146175133 26.81732267158251 23.56759776631143 84-85 22.454105966532016 26.615696164714798 26.825491701326694 24.104706167426492 86-87 22.537229707470324 26.547965898902742 27.111134853199903 23.80366954042703 88-89 22.382468411703694 26.644038915218015 26.921828316354535 24.051664356723755 90-91 22.35163230576252 26.732159138059846 27.107829081727992 23.80837947444964 92-93 22.49065441779007 26.473915757570353 26.988596458842306 24.04683336579727 94-95 22.266861917148827 26.37816614984276 27.045671574557645 24.309300358450773 96-97 22.298406381433853 26.310811000054745 27.045747839662955 24.34503477884845 98-99 22.053611326476442 26.489532396250137 26.766176058080315 24.690680219193105 100-101 22.035264188601886 26.136635495913723 27.363446141519947 24.464654173964437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 711.0 1 504.0 2 421.0 3 608.0 4 570.5 5 539.0 6 929.5 7 1972.0 8 3196.0 9 3601.0 10 3314.0 11 3145.5 12 3449.0 13 3889.0 14 4250.5 15 4603.0 16 4870.0 17 5053.5 18 5366.5 19 5798.0 20 6326.0 21 7183.5 22 8564.0 23 10711.0 24 13091.0 25 15812.5 26 19831.5 27 25233.0 28 30375.0 29 36040.5 30 43321.5 31 52105.0 32 62402.5 33 70586.5 34 77988.5 35 87647.5 36 99467.5 37 110394.0 38 116949.5 39 120666.5 40 125250.0 41 137804.5 42 151630.5 43 161074.0 44 169651.0 45 177908.0 46 178483.5 47 179527.0 48 196547.5 49 200336.5 50 188908.5 51 181514.5 52 174362.5 53 174507.0 54 176118.0 55 162808.5 56 141810.0 57 127587.0 58 116368.5 59 101226.0 60 86057.0 61 71630.5 62 58489.5 63 46451.5 64 36110.0 65 28008.0 66 21110.0 67 18245.5 68 13739.5 69 8454.5 70 6553.5 71 5424.5 72 4541.5 73 3874.0 74 3194.0 75 2630.5 76 1730.0 77 875.5 78 465.5 79 304.0 80 185.5 81 91.5 82 61.5 83 47.5 84 29.5 85 21.5 86 14.0 87 7.5 88 4.0 89 6.0 90 5.0 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27607076883513026 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0024188815346402933 16-17 5.304564768948011E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.1218259075792045E-5 26-27 0.0010396946947138102 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0034904036179677915 36-37 0.021769933811762638 38-39 0.007086898531314543 40-41 0.018099174991650615 42-43 0.005262128250796427 44-45 6.153295131979693E-4 46-47 2.6522823844740055E-4 48-49 0.0 50-51 0.0 52-53 1.0609129537896022E-5 54-55 0.0 56-57 0.0 58-59 4.5619257012952894E-4 60-61 0.0017823337623665318 62-63 0.0012837046740854187 64-65 2.1218259075792045E-5 66-67 0.0 68-69 5.304564768948011E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2333159767974093 78-79 0.0 80-81 0.0011563951196306664 82-83 0.0 84-85 0.0 86-87 0.10441505291197266 88-89 0.35898111617378775 90-91 0.3588325883602572 92-93 0.0 94-95 3.182738861368807E-5 96-97 0.0 98-99 0.0 100-101 2.2279172029581647E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4712922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.61325554769274 #Duplication Level Percentage of deduplicated Percentage of total 1 62.58135009590073 20.40981563197236 2 15.877125246296162 10.356094860403614 3 7.173292487833171 7.018332630721434 4 4.018587694576147 5.242369096961013 5 2.45203369142923 3.9984400695066915 6 1.5941598433161956 3.1194445412364535 7 1.1764756579675821 2.6858090895255695 8 0.8138957185121418 2.123503124560758 9 0.6160598787384418 1.8082546432179658 >10 3.233223050545008 18.854760755282136 >50 0.25536653295631806 5.813978326594053 >100 0.18829880726773218 11.927809587377894 >500 0.0149844590826411 3.3113131530179163 >1k 0.004951386126151427 2.713493970414753 >5k 1.3029963489872014E-4 0.34582392675903956 >10k+ 6.514981744936007E-5 0.2707565924484174 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12743 0.270384275402818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9491 0.2013824968883423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6785 0.14396588782924902 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 4719 0.10012896457866267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0609129537896022E-5 0.0 20-21 0.0 0.0 0.0 1.379186839926483E-4 0.0 22-23 0.0 0.0 0.0 2.864464975231926E-4 0.0 24-25 0.0 0.0 0.0 5.198473473569051E-4 0.0 26-27 0.0 0.0 0.0 9.22994269796954E-4 0.0 28-29 0.0 0.0 0.0 0.0026841097730876937 0.0 30-31 0.0 0.0 0.0 0.009696744397636965 0.0 32-33 0.0 0.0 0.0 0.022639882433870114 0.0 34-35 0.0 0.0 0.0 0.04270174639003149 0.0 36-37 0.0 0.0 0.0 0.07732994520172412 0.0 38-39 0.0 0.0 0.0 0.1323276727261771 0.0 40-41 0.0 0.0 0.0 0.19753138286608604 0.0 42-43 0.0 0.0 0.0 0.26474021848865736 0.0 44-45 0.0 0.0 0.0 0.3449028012769997 1.0609129537896022E-5 46-47 0.0 0.0 0.0 0.44400268029048645 2.1218259075792045E-5 48-49 0.0 0.0 0.0 0.5516535177115174 2.1218259075792045E-5 50-51 0.0 0.0 0.0 0.6757909424344388 2.1218259075792045E-5 52-53 0.0 0.0 0.0 0.7997267936961401 2.1218259075792045E-5 54-55 0.0 0.0 0.0 0.936871011232522 2.1218259075792045E-5 56-57 0.0 0.0 0.0 1.0987132823331258 2.1218259075792045E-5 58-59 0.0 0.0 0.0 1.285105079184421 2.1218259075792045E-5 60-61 0.0 0.0 0.0 1.491335948271582 2.1218259075792045E-5 62-63 0.0 0.0 0.0 1.7019589969874316 2.1218259075792045E-5 64-65 0.0 0.0 0.0 1.9234564034796247 2.1218259075792045E-5 66-67 0.0 0.0 0.0 2.1769615537876503 2.1218259075792045E-5 68-69 0.0 0.0 0.0 2.467460738794319 2.1218259075792045E-5 70-71 0.0 0.0 0.0 2.7959512166761935 2.1218259075792045E-5 72-73 0.0 0.0 0.0 3.131114837037405 2.1218259075792045E-5 74-75 0.0 0.0 0.0 3.4819905782442397 2.1218259075792045E-5 76-77 0.0 0.0 0.0 3.8645663985103083 2.1218259075792045E-5 78-79 0.0 0.0 0.0 4.294384672608627 2.1218259075792045E-5 80-81 0.0 0.0 0.0 4.773959764239679 2.1218259075792045E-5 82-83 0.0 0.0 0.0 5.266562866943268 2.1218259075792045E-5 84-85 0.0 0.0 0.0 5.772735046325825 2.1218259075792045E-5 86-87 0.0 0.0 0.0 6.304729847003621 2.1218259075792045E-5 88-89 0.0 0.0 0.0 6.877739542474924 2.1218259075792045E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13990 0.0 31.154078 1 GTATCAA 20195 0.0 31.100489 1 GTACATG 22000 0.0 27.921625 1 TACATGG 22480 0.0 26.892944 2 ACATGGG 22645 0.0 25.586365 3 ATCAACG 25240 0.0 24.572622 3 TCAACGC 25260 0.0 24.515593 4 TATCAAC 25475 0.0 24.420454 2 CAACGCA 25530 0.0 24.25632 5 AACGCAG 26100 0.0 23.78113 6 CATGGGA 12245 0.0 21.159191 4 CATGGGG 10445 0.0 20.76218 4 GTATCGC 115 0.0037463133 20.631813 6 ACGCAGA 29920 0.0 20.586302 7 GAGTACT 13375 0.0 20.579351 12-13 CCCTATA 765 0.0 20.469994 2 CGCAGAG 30160 0.0 20.343817 8 GTACTTT 13890 0.0 19.543001 14-15 AGTACAT 16420 0.0 19.333843 2 CCTATAC 965 0.0 19.177965 3 >>END_MODULE