##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939333_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1423612 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07307749583454 33.0 31.0 34.0 30.0 34.0 2 32.076923347091764 33.0 31.0 34.0 30.0 34.0 3 32.134791642666684 34.0 31.0 34.0 30.0 34.0 4 35.58274726540659 37.0 35.0 37.0 33.0 37.0 5 35.65821305243283 37.0 35.0 37.0 35.0 37.0 6 35.819117849526414 37.0 35.0 37.0 35.0 37.0 7 35.7944292405515 37.0 35.0 37.0 35.0 37.0 8 35.80924928983459 37.0 36.0 37.0 35.0 37.0 9 37.390305083126584 39.0 37.0 39.0 35.0 39.0 10-11 37.29931013506489 39.0 37.0 39.0 34.5 39.0 12-13 37.26814855452187 39.0 37.0 39.0 34.0 39.0 14-15 38.34867189936584 40.0 38.0 41.0 34.0 41.0 16-17 38.366947244052454 40.0 38.0 41.0 34.0 41.0 18-19 38.46026375164019 40.0 38.0 41.0 34.0 41.0 20-21 38.47029878927685 40.0 38.0 41.0 34.0 41.0 22-23 38.47825179894522 40.0 38.0 41.0 34.0 41.0 24-25 38.36055891633394 40.0 38.0 41.0 34.0 41.0 26-27 38.20940677656553 40.0 38.0 41.0 34.0 41.0 28-29 38.11375782165365 40.0 38.0 41.0 34.0 41.0 30-31 37.96491213898169 40.0 37.5 41.0 34.0 41.0 32-33 37.64692978142921 40.0 37.0 41.0 33.0 41.0 34-35 37.24397483303035 40.0 36.0 41.0 33.0 41.0 36-37 36.939976622843865 39.5 35.5 41.0 31.5 41.0 38-39 36.33572244403672 38.5 35.0 40.5 30.5 41.0 40-41 36.41569718434518 39.0 35.0 40.5 30.5 41.0 42-43 36.4165116618854 39.5 35.0 41.0 30.0 41.0 44-45 36.11317058299593 40.0 35.0 41.0 30.0 41.0 46-47 35.56472936446167 40.0 35.0 41.0 26.5 41.0 48-49 35.1064173384321 39.0 35.0 41.0 23.5 41.0 50-51 33.86726018044243 38.0 34.0 40.0 21.0 40.5 52-53 34.04988402738948 38.5 34.5 39.5 20.5 40.5 54-55 34.620076959171456 39.0 35.0 40.5 20.0 41.0 56-57 34.445658648564354 39.0 35.0 41.0 17.0 41.0 58-59 34.29113058895261 39.0 35.0 41.0 14.5 41.0 60-61 33.86587075691972 38.5 34.0 40.0 10.0 41.0 62-63 33.56561513951835 38.0 34.0 40.0 8.0 41.0 64-65 33.18920499405737 37.5 33.5 40.0 4.0 41.0 66-67 32.66537336015712 37.0 33.0 39.0 2.0 41.0 68-69 32.17008566940992 36.5 32.0 39.0 2.0 40.5 70-71 31.602317204406816 36.0 31.5 38.5 2.0 40.0 72-73 31.09298917120676 35.0 31.0 37.5 2.0 40.0 74-75 30.646095284389286 35.0 31.0 37.0 2.0 39.0 76-77 30.195998980059173 35.0 31.0 36.5 2.0 39.0 78-79 29.754094163297303 35.0 30.0 36.0 2.0 38.5 80-81 29.18718794165826 34.0 29.0 36.0 2.0 37.5 82-83 28.744254754806786 34.0 29.0 35.0 2.0 37.0 84-85 28.281255356094217 34.0 29.0 35.0 2.0 36.5 86-87 27.991175264046664 34.0 29.0 35.0 2.0 36.0 88-89 27.447227193926437 34.0 26.5 35.0 2.0 36.0 90-91 26.89910347763295 34.0 25.0 35.0 2.0 35.0 92-93 26.416388735132887 33.5 25.0 35.0 2.0 35.0 94-95 25.89356334450679 33.0 23.5 35.0 2.0 35.0 96-97 25.22591338089311 33.0 18.5 35.0 2.0 35.0 98-99 24.531120136666452 32.5 2.0 35.0 2.0 35.0 100-101 21.916890276283144 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2853711220128048 1101 2 0.4153690175010638 1101 3 0.3621531699013474 1101 4 0.30097381378387666 1101 5 0.285364492492306 1101 6 0.3810514983521074 1101 7 0.405516569044174 1101 8 0.20264181159065942 1101 9 0.2788906128113453 1101 10-11 0.4916436554160697 1101 12-13 0.3753515007426955 1101 14-15 0.5389969153591849 1101 16-17 0.6309365561208651 1101 18-19 0.498784914645789 1101 20-21 0.5734025455736074 1101 22-23 0.5705931939729822 1101 24-25 0.5126974335260854 1101 26-27 0.5266181295230794 1101 28-29 0.5570888174023807 1101 30-31 0.6099000831866164 1101 32-33 0.5129454757092802 1101 34-35 0.5880897324355487 1101 36-37 0.5117344790859164 1101 38-39 0.9838374120659026 1101 40-41 0.7165721704221895 1101 42-43 0.7159668096386369 1101 44-45 0.6100233143278828 1101 46-47 0.9831836380442596 1101 48-49 0.9685786782175683 1101 50-51 1.010567847063946 1101 52-53 0.9769792627611551 1101 54-55 0.8730120548285569 1101 56-57 0.9487134251491014 1101 58-59 0.8278734724223611 1101 60-61 0.7730121327373141 1101 62-63 0.8821392119618352 1101 64-65 0.7131988841844219 1101 66-67 0.6364947483564407 1101 68-69 0.7096125676557392 1101 70-71 0.6835961218611999 1101 72-73 0.7921057600140315 1101 74-75 0.9063242567579124 1101 76-77 0.8688121759425727 1101 78-79 0.64330246275712 1101 80-81 0.546454368507522 1101 82-83 0.4255059294324752 1101 84-85 0.36080547605428137 1101 86-87 0.38670434838476453 1101 88-89 0.027475891937232433 1101 90-91 0.2535769190306034 1101 92-93 0.30759676204457165 1101 94-95 0.4151684588812543 1101 96-97 0.44455435581825853 1101 98-99 0.8115606457294042 1101 100-101 0.6350639007466867 1102 1 0.3610783614718329 1102 2 0.26788262131967855 1102 3 0.37066231866825206 1102 4 0.25084366263033786 1102 5 0.28199074547560343 1102 6 0.3638810926241831 1102 7 0.3379417082645446 1102 8 0.29561110355565035 1102 9 0.47315314264427855 1102 10-11 0.5246457238329327 1102 12-13 0.44465587623991354 1102 14-15 0.6013402725588648 1102 16-17 0.6503192132409907 1102 18-19 0.48391363381841757 1102 20-21 0.4686346855895209 1102 22-23 0.6126899322386947 1102 24-25 0.5177984677345151 1102 26-27 0.5833283920529198 1102 28-29 0.6045602573387399 1102 30-31 0.6140101866074517 1102 32-33 0.6795367247148505 1102 34-35 0.622870002920834 1102 36-37 0.7080320128965738 1102 38-39 1.126553084301385 1102 40-41 0.9654564981867111 1102 42-43 0.8856689576099939 1102 44-45 0.881799925306403 1102 46-47 0.9986776714571448 1102 48-49 0.9977907705007851 1102 50-51 0.9835151819883663 1102 52-53 0.978455475967202 1102 54-55 0.8742721980266523 1102 56-57 1.0493676813145782 1102 58-59 0.8993038622393854 1102 60-61 0.9954630476140096 1102 62-63 0.8583275970055908 1102 64-65 0.6618954633411462 1102 66-67 0.8605542551185792 1102 68-69 0.8038178182523978 1102 70-71 1.0592355808906433 1102 72-73 1.0671355133950975 1102 74-75 0.9155140737825747 1102 76-77 0.8873000041048655 1102 78-79 1.012872550267062 1102 80-81 0.9946220693826184 1102 82-83 0.9648971784380436 1102 84-85 0.9372675285602483 1102 86-87 0.9264717310418824 1102 88-89 0.7777400128600611 1102 90-91 0.933661246795122 1102 92-93 0.8612263563166458 1102 94-95 1.0009215217293068 1102 96-97 1.220052526064876 1102 98-99 1.3441703513777732 1102 100-101 1.1491316811762822 1103 1 0.26707358820214466 1103 2 0.3063793595853923 1103 3 0.3366957315560839 1103 4 0.35347978037098926 1103 5 0.3354838242345508 1103 6 0.2712662954881466 1103 7 0.2885714616456099 1103 8 0.21696162065986613 1103 9 0.3966312651581987 1103 10-11 0.42601119718217717 1103 12-13 0.3375631156989414 1103 14-15 0.6026405112223472 1103 16-17 0.5668951877835511 1103 18-19 0.6069790276131712 1103 20-21 0.6306817818504626 1103 22-23 0.498198603678631 1103 24-25 0.5731588495960906 1103 26-27 0.5500867054976197 1103 28-29 0.5582821348248146 1103 30-31 0.4042517140537569 1103 32-33 0.49584125932105394 1103 34-35 0.48466888915790207 1103 36-37 0.48150377105091025 1103 38-39 0.804442026226603 1103 40-41 0.5841139365319776 1103 42-43 0.5377647412217712 1103 44-45 0.33237812737481676 1103 46-47 0.4593172895955746 1103 48-49 0.7092310250751694 1103 50-51 0.5077834397449266 1103 52-53 0.6583635109711778 1103 54-55 0.8753986896734247 1103 56-57 0.7390873312748028 1103 58-59 0.7264414915154447 1103 60-61 0.7654544557285661 1103 62-63 0.5909697608878517 1103 64-65 0.5091416049481481 1103 66-67 0.5684756552776875 1103 68-69 0.5703922017050616 1103 70-71 0.4516947694347948 1103 72-73 0.5999816550020967 1103 74-75 0.6195303028995198 1103 76-77 0.469846384375348 1103 78-79 0.543461571746775 1103 80-81 0.45178451966105726 1103 82-83 0.5722839723919009 1103 84-85 0.5314498674623955 1103 86-87 0.4288682306441096 1103 88-89 0.4093374671162273 1103 90-91 0.543553052682153 1103 92-93 0.5597845106523671 1103 94-95 0.5086449903052817 1103 96-97 0.5885479914586718 1103 98-99 0.5128335176466692 1103 100-101 0.10045769549609318 1104 1 0.21389119569643356 1104 2 0.3076112691424484 1104 3 0.2826928763458696 1104 4 0.1909072225997761 1104 5 0.2109667576986709 1104 6 0.2218325059167725 1104 7 0.3020969412668322 1104 8 0.27248749774969383 1104 9 0.3003830891683421 1104 10-11 0.38642326393988924 1104 12-13 0.4178283743300568 1104 14-15 0.4074004711837702 1104 16-17 0.34812822775588614 1104 18-19 0.3732401426037484 1104 20-21 0.445050575581881 1104 22-23 0.4857096419407583 1104 24-25 0.529605954365536 1104 26-27 0.517432593733588 1104 28-29 0.4310772932440159 1104 30-31 0.5283594608626103 1104 32-33 0.4501460448065231 1104 34-35 0.5606732114769812 1104 36-37 0.5346156645390536 1104 38-39 0.9843810445764021 1104 40-41 0.7928047029231493 1104 42-43 0.5980316772433056 1104 44-45 0.601785868774698 1104 46-47 0.7114263345587517 1104 48-49 0.6306668591180511 1104 50-51 0.6909043340128065 1104 52-53 0.5896111673346525 1104 54-55 0.6991705937485833 1104 56-57 0.7264579792519399 1104 58-59 0.7355483236603177 1104 60-61 0.7570447130074385 1104 62-63 0.8496449384876357 1104 64-65 0.8252497796131095 1104 66-67 0.9276256072958091 1104 68-69 0.6840378335312352 1104 70-71 0.880522364695068 1104 72-73 0.7357995317297039 1104 74-75 0.5448882525449861 1104 76-77 0.7843738008963044 1104 78-79 0.8048701056528529 1104 80-81 0.727355759222192 1104 82-83 0.7484971784380434 1104 84-85 0.8348278035793477 1104 86-87 0.7678153521266786 1104 88-89 0.6281342069013149 1104 90-91 0.6743472666576089 1104 92-93 0.7793938123899835 1104 94-95 0.9764530725314451 1104 96-97 1.0582309829021561 1104 98-99 0.9673905194450008 1104 100-101 0.7619976857599333 1105 1 0.16955056253809175 1105 2 0.2515182648840337 1105 3 0.25774228820366574 1105 4 0.24484396727618218 1105 5 0.22195053222350936 1105 6 0.27953135918930627 1105 7 0.17379425967352802 1105 8 0.27599069228375583 1105 9 0.4147433939771048 1105 10-11 0.43717245650620384 1105 12-13 0.36160911310205535 1105 14-15 0.4514618262526895 1105 16-17 0.3275652147642205 1105 18-19 0.30587768560821615 1105 20-21 0.3774228044013981 1105 22-23 0.43200417443214434 1105 24-25 0.4315775994938491 1105 26-27 0.37840592442153564 1105 28-29 0.47695599229685115 1105 30-31 0.47654057503090996 1105 32-33 0.6367745008001506 1105 34-35 0.4903725162490815 1105 36-37 0.48547755745102705 1105 38-39 0.8420577301505858 1105 40-41 0.7460136954448942 1105 42-43 0.6397889880745851 1105 44-45 0.6638952794572006 1105 46-47 0.7120942746559251 1105 48-49 0.9094614483377939 1105 50-51 0.7364068803889765 1105 52-53 0.7444871591355167 1105 54-55 0.7548845361835461 1105 56-57 0.8218721748408555 1105 58-59 0.8741005111350475 1105 60-61 0.8716342585812598 1105 62-63 0.745517848338416 1105 64-65 0.7627298883221059 1105 66-67 0.747998885735484 1105 68-69 0.5964728068281744 1105 70-71 0.6558774696949001 1105 72-73 0.6643087045603657 1105 74-75 0.8949775924421317 1105 76-77 0.7611364092838429 1105 78-79 0.7490139059410907 1105 80-81 0.8056493351861214 1105 82-83 0.8235162188036185 1105 84-85 0.840150087585382 1105 86-87 0.6626572603640462 1105 88-89 0.42797763662244037 1105 90-91 0.4715180632459983 1105 92-93 0.6988412839632545 1105 94-95 0.852692732696557 1105 96-97 0.9019178725995296 1105 98-99 1.1325072896869877 1105 100-101 0.6806253597749112 1106 1 0.22121708979553745 1106 2 0.18482250571274506 1106 3 0.22777792560466992 1106 4 0.2525800788153063 1106 5 0.15237340443513858 1106 6 0.21205912730626864 1106 7 0.2589960435246539 1106 8 0.31041688390247657 1106 9 0.3189219287714451 1106 10-11 0.3566231804803337 1106 12-13 0.3673963386676604 1106 14-15 0.3911327581079931 1106 16-17 0.4548943577496587 1106 18-19 0.4681610326623442 1106 20-21 0.5543994254739104 1106 22-23 0.5617309727011204 1106 24-25 0.5544718781391467 1106 26-27 0.5282434209546523 1106 28-29 0.5756591012693733 1106 30-31 0.6065992039484911 1106 32-33 0.5176563778940348 1106 34-35 0.4461752052329686 1106 36-37 0.35445397821450086 1106 38-39 0.7241230264979208 1106 40-41 0.6941333767994209 1106 42-43 0.5563140443152008 1106 44-45 0.5408617750173832 1106 46-47 0.4212846078733321 1106 48-49 0.40040984564529225 1106 50-51 0.46960073112131084 1106 52-53 0.6321352447533286 1106 54-55 0.6205814465815607 1106 56-57 0.5729017853608198 1106 58-59 0.6314888333376558 1106 60-61 0.7540110244926197 1106 62-63 0.5355345334217745 1106 64-65 0.49420818010414536 1106 66-67 0.47599240540759524 1106 68-69 0.30461839628708276 1106 70-71 0.25861014736462806 1106 72-73 0.29025054229682823 1106 74-75 0.5292718772507818 1106 76-77 0.5515699463013952 1106 78-79 0.5491910473768868 1106 80-81 0.48719316764851683 1106 82-83 0.35037579115480355 1106 84-85 0.45433862682614645 1106 86-87 0.688978089423383 1106 88-89 0.5922024406057282 1106 90-91 0.4374242525754646 1106 92-93 0.4547171077617307 1106 94-95 0.5208353945616757 1106 96-97 0.796862930689155 1106 98-99 1.003314282013612 1106 100-101 1.0789883285751252 1107 1 0.09439553894866748 1107 2 0.0882401409205329 1107 3 0.1691056543774465 1107 4 0.16328443240937673 1107 5 0.18738147961672524 1107 6 0.1272686906284619 1107 7 0.0555941672809368 1107 8 0.08117803156705605 1107 9 0.25415670643615584 1107 10-11 0.26998827550791304 1107 12-13 0.2502065533603641 1107 14-15 0.2670121186030556 1107 16-17 0.3338858561490454 1107 18-19 0.3956903978954003 1107 20-21 0.2499766670578012 1107 22-23 0.1522391838424042 1107 24-25 0.2311641127808528 1107 26-27 0.273321834675869 1107 28-29 0.29697907415984304 1107 30-31 0.3249399086316913 1107 32-33 0.38404791502133406 1107 34-35 0.4800152630776324 1107 36-37 0.35718810698067927 1107 38-39 0.6834494492336773 1107 40-41 0.49878479469418835 1107 42-43 0.44439140949880596 1107 44-45 0.48358695310397337 1107 46-47 0.5564795246289265 1107 48-49 0.5731803642285058 1107 50-51 0.6103653601779584 1107 52-53 0.6500360889394017 1107 54-55 0.7539329250401963 1107 56-57 0.7175860704806567 1107 58-59 0.7847978037932037 1107 60-61 0.6395947653616929 1107 62-63 0.7631207545251897 1107 64-65 0.5696670955578256 1107 66-67 0.4591849037286977 1107 68-69 0.4925818952303018 1107 70-71 0.3641071418314752 1107 72-73 0.358297594649553 1107 74-75 0.4913826411382374 1107 76-77 0.5194049221376495 1107 78-79 0.5878558717210893 1107 80-81 0.4062847921196102 1107 82-83 0.3878414407331263 1107 84-85 0.3611797167284614 1107 86-87 0.4843474259812979 1107 88-89 0.49721399717937587 1107 90-91 0.49872610780724 1107 92-93 0.5612979172574875 1107 94-95 0.6352647861626615 1107 96-97 0.6041189551454202 1107 98-99 0.7580252337726421 1107 100-101 0.6163926932931751 1108 1 0.02782836147183332 1108 2 0.12147151020857194 1108 3 0.14613454089047906 1108 4 0.16092144040811007 1108 5 0.0913907454756 1108 6 0.06148109262418444 1108 7 0.13465837493120603 1108 8 0.1939388813334304 1108 9 0.25234758708872107 1108 10-11 0.2663735016107154 1108 12-13 0.2607308762399114 1108 14-15 0.3222763836699798 1108 16-17 0.3333886576854397 1108 18-19 0.3163747449295258 1108 20-21 0.3559569078117448 1108 22-23 0.24612604334980404 1108 24-25 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-0.7504072113974871 2316 60-61 -0.6228942317564261 2316 62-63 -0.9634338420050952 2316 64-65 -1.1257971729713887 2316 66-67 -1.1227462507836137 2316 68-69 -1.0815964594316938 2316 70-71 -0.8250160211329742 2316 72-73 -0.9051088935751146 2316 74-75 -0.8114028458351896 2316 76-77 -0.894803649633193 2316 78-79 -1.0432776464726814 2316 80-81 -1.1036239115055189 2316 82-83 -1.082306643934075 2316 84-85 -0.8659261757680277 2316 86-87 -0.9730657619316965 2316 88-89 -1.2568603244080663 2316 90-91 -0.9375034524741288 2316 92-93 -1.1306358134416428 2316 94-95 -1.3124372191363491 2316 96-97 -1.2903333086737412 2316 98-99 -1.4455928863962555 2316 100-101 -1.123285631920961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5975.0 3 834.0 4 838.0 5 873.0 6 1006.0 7 1195.0 8 1490.0 9 1987.0 10 2411.0 11 3745.0 12 5922.0 13 9241.0 14 9930.0 15 12467.0 16 17903.0 17 25470.0 18 20307.0 19 10537.0 20 9583.0 21 9182.0 22 9825.0 23 10582.0 24 12019.0 25 13494.0 26 15499.0 27 18131.0 28 21494.0 29 25203.0 30 30479.0 31 37380.0 32 46631.0 33 60297.0 34 81965.0 35 119377.0 36 178734.0 37 278857.0 38 270676.0 39 42060.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.0954869880776 20.480911690545987 15.327149156456576 17.096452164919835 2 13.48393280376515 25.57254164578982 37.35686458855255 23.586660961892484 3 25.029713839365826 27.52778745368758 31.72924341422394 15.71325529272266 4 10.558596147457445 17.26697241507458 44.0605697168308 28.113861720637175 5 9.459912261436141 43.030401581321485 34.9029694829732 12.606716674269174 6 23.94753756207605 40.53961686190179 17.520108350925355 17.992737225096796 7 22.80557020782783 37.585891620072445 22.74508562787917 16.863452544220557 8 23.27106314194593 28.599785883203648 21.126589170079356 27.002561804771062 9 22.839301654697667 22.127773920591675 26.288470155579425 28.744454269131232 10-11 23.796856982395038 26.133776062721093 27.47378034606462 22.595586608819257 12-13 29.733405722901736 25.87205050016316 26.305122030808697 18.08942174612641 14-15 23.544648368054276 27.380991318446146 32.1368017345523 16.937558578947275 16-17 17.252429191647717 30.584996041004782 31.766471318732165 20.396103448615335 18-19 16.37601619225311 33.328432984505866 32.6743419259426 17.62120889729842 20-21 20.224523439132554 27.36936571829418 30.41194643971583 21.99416440285743 22-23 17.94463435802233 27.814183254934616 32.11223417522685 22.1289482118162 24-25 22.05018525028406 28.140448886650894 31.117191697887737 18.69217416517731 26-27 20.650881765931224 24.82760294833172 36.98255817839792 17.538957107339133 28-29 16.752583938647575 32.03362717474247 33.367751840569255 17.846037046040706 30-31 18.251153477599527 28.144114696268485 32.34268169646689 21.262050129665095 32-33 18.97700027265451 28.805403836512838 33.28531095807277 18.93228493275988 34-35 21.815264175175976 26.951760909376716 32.20340297270206 19.029571942745246 36-37 20.22533008549741 25.821707075829064 35.9059482611692 18.047014577504324 38-39 19.14678063807669 28.943127057639117 34.275060205731286 17.635032098552912 40-41 18.287768109398865 30.020739789709822 33.58766617403787 18.103825926853442 42-43 16.890671867425798 30.790860192671754 30.06035016218268 22.25811777771976 44-45 19.253935447997893 27.32078355441746 30.86411564349841 22.561165354086242 46-47 22.11084264913635 30.022630309513765 30.31882838928931 17.54769865206058 48-49 20.68059132268471 28.857080550672247 30.8878379684945 19.574490158148542 50-51 16.35996423588789 30.18087410369193 34.78451557905329 18.674646081366884 52-53 18.95168006142676 32.65410325680394 29.02178024689267 19.372436434876626 54-55 16.909242678795586 28.766760818193944 31.454701073016945 22.86929542999353 56-57 20.48956842182109 28.24459517746044 29.82535213651883 21.44048426419964 58-59 23.45679142951851 27.569487054708052 29.324418678458837 19.6493028373146 60-61 19.672321259251895 29.018190431676434 33.61585147053951 17.69363683853216 62-63 18.40661812574651 29.118518296351315 32.69822002903987 19.77664354886231 64-65 17.604216690407544 32.873911768618356 30.590659161326588 18.931212379647512 66-67 18.970346251526657 28.925646665422168 28.633107335820686 23.470899747230494 68-69 17.04715817172091 29.635457987357107 30.566276387598784 22.751107453323208 70-71 21.266859582877508 29.282457564315205 28.7707943677877 20.679888485019585 72-73 19.694653579383296 26.185823332966347 33.46191956232692 20.65760352532344 74-75 17.10627167617819 33.09155763587267 29.63106209567796 20.171108592271175 76-77 18.58195617215873 28.238242709177854 29.896304544014473 23.283496574648947 78-79 18.723981731255293 28.669561416783694 31.413118516488186 21.193338335472824 80-81 20.823513683309717 28.228808516207227 29.805384854439893 21.142292946043163 82-83 18.874628823379375 27.353419411148156 32.85041419024493 20.92153757522754 84-85 18.226135574659764 28.85180941199049 31.458960131408865 21.46309488194088 86-87 18.176750651362084 29.627599053783015 30.859727389312546 21.335922905542354 88-89 18.025920066387886 29.110820068222587 28.85216275758119 24.01109710780834 90-91 18.84007338734693 26.11260560723654 30.676835592369212 24.370485413047323 92-93 20.753353303049384 28.859933428005974 30.62714036865865 19.759572900285992 94-95 19.324793440052005 27.55652453469533 30.850508578770864 22.2681734464818 96-97 15.935073422817467 27.595806789934375 33.60613101346497 22.86298877378319 98-99 18.699206787790505 29.10409154652738 29.057546173081032 23.139155492601084 100-101 17.763972174904634 26.069161930595325 31.372350469749595 24.79451542475045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4016.0 1 2369.0 2 814.0 3 882.0 4 768.0 5 823.5 6 1422.5 7 2626.5 8 3795.5 9 4129.0 10 3750.0 11 3441.0 12 3584.5 13 3772.0 14 3924.0 15 4195.0 16 4515.0 17 4770.5 18 5023.5 19 5392.5 20 5925.5 21 6691.5 22 7477.5 23 8159.0 24 9062.0 25 10733.5 26 13975.0 27 17529.0 28 18698.5 29 19764.5 30 23050.0 31 26124.0 32 29790.0 33 35039.0 34 41392.0 35 45560.5 36 49669.5 37 54627.5 38 51965.0 39 45905.5 40 41705.5 41 38238.5 42 35111.0 43 33438.0 44 32977.0 45 33423.0 46 42343.5 47 83519.5 48 176891.5 49 170435.5 50 69408.0 51 25322.0 52 13144.0 53 9040.0 54 6753.0 55 5368.5 56 4413.5 57 3553.0 58 2743.0 59 2122.0 60 1626.0 61 1365.0 62 1187.5 63 997.5 64 825.5 65 713.5 66 637.5 67 577.5 68 496.5 69 429.0 70 356.5 71 303.5 72 257.5 73 187.0 74 145.0 75 121.0 76 87.0 77 57.5 78 46.5 79 27.0 80 19.5 81 16.5 82 10.0 83 8.5 84 4.5 85 2.0 86 4.0 87 3.0 88 0.0 89 0.0 90 2.0 91 2.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2938300604378159 2 0.18122915513496654 3 0.17961354638763935 4 0.1992115829313043 5 0.21269840377855764 6 0.11000188253540992 7 0.12376967881698103 8 0.13753747509855213 9 0.14210332590621602 10-11 0.12823016383677577 12-13 0.1177287069791488 14-15 0.13483308654324352 16-17 0.10775407906086772 18-19 0.13658918300772963 20-21 0.16401940978300267 22-23 0.1651784334495635 24-25 0.18941256465947182 26-27 0.1686906263785357 28-29 0.16721550534836738 30-31 0.15861063267238545 32-33 0.16855013866137683 34-35 0.34911197713983866 36-37 0.46842117093702496 38-39 0.43414216795025606 40-41 0.42476461282990025 42-43 0.4483665493125936 44-45 0.451597766807248 46-47 0.45560166674627633 48-49 0.46733239112904357 50-51 0.4600270298367814 52-53 0.4664192209675108 54-55 0.44906898789838806 56-57 0.41257730336636667 58-59 0.4019353587915809 60-61 0.35957831206817586 62-63 0.46322312540214605 64-65 0.46195873594771614 66-67 0.4712309252802027 68-69 0.4810299435520352 70-71 0.474988971714203 72-73 0.4681050735734175 74-75 0.47302214367397855 76-77 0.44850703702975253 78-79 0.436811434576275 80-81 0.4336504609402 82-83 0.4445382590200139 84-85 0.4464699651309486 86-87 0.44882313439336 88-89 0.45560166674627633 90-91 0.4739001919062217 92-93 0.4776582383402219 94-95 0.4756562883707078 96-97 0.4741460454112497 98-99 0.4716875103609692 100-101 0.45475874044332304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1423612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.06630352403419 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57400654698642 18.69294683110075 2 12.163206176687703 6.097732357005229 3 4.628977426452944 3.480940595321165 4 2.2716952188003017 2.277720074741825 5 1.3461034124294384 1.687091835534724 6 0.8845784770130607 1.3303867557382367 7 0.599026804082586 1.0510771383116386 8 0.4260816576237381 0.8544233724816189 9 0.33037451253391187 0.7453141027001887 >10 2.1201314798238493 10.567680855439054 >50 0.3405429822511263 5.934981574969974 >100 0.27456745200221583 13.477734395555407 >500 0.021898017644109154 3.6565341617314004 >1k 0.011510496453954813 5.526954128718678 >5k 0.0039304134233016435 7.298782787765506 >10k+ 0.003368925791401408 17.319699032884632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 57174 4.0161223704211535 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34732 2.439709696181263 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 26063 1.8307656861560593 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19703 1.384014745590793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15310 1.075433474851294 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14948 1.050005198045535 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14254 1.0012559601914006 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 14030 0.9855213358696049 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12943 0.9091662615937489 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12807 0.8996130968269443 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12328 0.8659662885673906 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11824 0.8305633838433506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9417 0.6614864162426279 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9415 0.661345928525469 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8961 0.6294552167304013 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8592 0.6035352329145862 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7961 0.5592113581509569 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7959 0.559070870433798 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7749 0.5443196601321146 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7524 0.5285147919517397 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6890 0.48398018561237194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6638 0.46627873325035196 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6141 0.4313675355363681 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6004 0.4217441269109842 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5369 0.377139276713037 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5097 0.3580329471794281 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4721 0.331621256353557 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4261 0.29930908140701257 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3558 0.24992764882566318 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3513 0.24676667518958817 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3283 0.23061058771631596 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3255 0.22864375967609152 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3128 0.21972278963650207 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2623 0.18424964105388267 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2621 0.18410915333672379 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2341 0.16444087293447934 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2319 0.16289550804573155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2173 0.15263990469313268 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2159 0.15165649067302045 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2109 0.14814429774404825 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2037 0.14308673992632823 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1938 0.13613259792696325 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.1327608927151499 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1850 0.12995113837197214 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1840 0.1292486997861777 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1708 0.11997651045369104 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.11323310003006437 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1521 0.10684090889933494 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1448 0.10171310722303549 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 7.02438585794444E-5 9 0.0 0.0 0.0 0.0 7.02438585794444E-5 10-11 0.0 0.0 0.0 1.75609646448611E-4 7.02438585794444E-5 12-13 0.0 0.0 0.0 2.458535050280554E-4 7.02438585794444E-5 14-15 0.0 0.0 0.0 3.51219292897222E-4 7.02438585794444E-5 16-17 0.0 0.0 0.0 7.375605150841662E-4 7.02438585794444E-5 18-19 0.0 0.0 0.0 8.780482322430551E-4 7.02438585794444E-5 20-21 0.0 0.0 0.0 0.001229267525140277 7.02438585794444E-5 22-23 0.0 0.0 0.0 0.002282925403831943 7.02438585794444E-5 24-25 0.0 0.0 0.0 0.00431999730263583 7.02438585794444E-5 26-27 0.0 0.0 0.0 0.00783219023160805 7.02438585794444E-5 28-29 0.0 0.0 0.0 0.01864974445284249 7.02438585794444E-5 30-31 0.0 0.0 0.0 0.049838017662115805 7.02438585794444E-5 32-33 0.0 0.0 0.0 0.11179310092918576 7.02438585794444E-5 34-35 0.0 0.0 0.0 0.20750035824367874 7.02438585794444E-5 36-37 0.0 0.0 0.0 0.33095393969705234 7.02438585794444E-5 38-39 0.0 0.0 0.0 0.5078279756000933 7.02438585794444E-5 40-41 0.0 0.0 0.0 0.6993478560169485 7.02438585794444E-5 42-43 0.0 0.0 0.0 0.8995077310390753 7.02438585794444E-5 44-45 0.0 0.0 0.0 1.1263953942506806 7.02438585794444E-5 46-47 0.0 0.0 0.0 1.4232459406074127 7.02438585794444E-5 48-49 0.0 0.0 0.0 1.7026408881071529 7.02438585794444E-5 50-51 0.0 0.0 0.0 2.011608500068839 7.02438585794444E-5 52-53 0.0 0.0 0.0 2.3053683166480754 7.02438585794444E-5 54-55 0.0 0.0 0.0 2.6350227449614083 7.02438585794444E-5 56-57 0.0 0.0 0.0 3.0823707583245996 7.02438585794444E-5 58-59 0.0 0.0 0.0 3.501937325619621 7.02438585794444E-5 60-61 0.0 0.0 0.0 3.9972267724632835 7.02438585794444E-5 62-63 0.0 0.0 0.0 4.46666647934971 7.02438585794444E-5 64-65 0.0 0.0 0.0 4.925920826742118 7.02438585794444E-5 66-67 0.0 0.0 0.0 5.483130234923561 7.02438585794444E-5 68-69 0.0 0.0 0.0 6.142649823125964 7.02438585794444E-5 70-71 0.0 0.0 0.0 6.830442564406594 7.02438585794444E-5 72-73 0.0 0.0 0.0 7.467484117863576 7.02438585794444E-5 74-75 0.0 0.0 0.0 8.201181220725871 7.02438585794444E-5 76-77 0.0 3.51219292897222E-5 0.0 8.863299831695715 7.02438585794444E-5 78-79 0.0 7.02438585794444E-5 0.0 9.812856311972645 7.02438585794444E-5 80-81 0.0 7.02438585794444E-5 0.0 10.820644951011932 7.02438585794444E-5 82-83 0.0 7.02438585794444E-5 0.0 11.671579053843324 7.02438585794444E-5 84-85 0.0 7.02438585794444E-5 0.0 12.629705284866944 7.02438585794444E-5 86-87 0.0 7.02438585794444E-5 0.0 13.587058833446193 7.02438585794444E-5 88-89 0.0 7.02438585794444E-5 0.0 14.586383087526658 7.02438585794444E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGACG 25 0.00507015 56.86137 6 GTAAGCG 255 0.0 37.284943 94-95 CTTTGCG 255 0.0 37.190453 2 CATTCCG 215 0.0 33.057774 9 GCTTTGC 335 0.0 28.338078 1 TTGCGTT 340 0.0 27.892843 4 TGGACGC 35 0.008360673 27.164743 94-95 TTTGCGT 355 0.0 26.714272 3 GCTTATG 310 0.0 26.029896 1 GAGTACT 10060 0.0 25.198168 12-13 ATGGGGA 1910 0.0 24.57783 5 GTACATG 10670 0.0 24.022299 1 CATGGGG 3790 0.0 23.771471 4 GTACTTT 10715 0.0 23.621078 14-15 AGTACTT 10365 0.0 23.496706 12-13 GGCATTC 285 3.6379788E-12 23.276587 7 TACATGG 10835 0.0 23.01964 2 ACATGGG 10265 0.0 23.004461 3 CGTGCAA 150 3.0922776E-5 22.111977 9 TGACCGT 155 3.95977E-5 21.414503 5 >>END_MODULE