##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939333_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1423612 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14881512659348 33.0 31.0 34.0 30.0 34.0 2 32.3255767723228 34.0 31.0 34.0 30.0 34.0 3 32.41443244367145 34.0 31.0 34.0 30.0 34.0 4 35.923564145286775 37.0 35.0 37.0 35.0 37.0 5 35.89591335279557 37.0 35.0 37.0 35.0 37.0 6 35.994751378886946 37.0 35.0 37.0 35.0 37.0 7 35.95526730597944 37.0 35.0 37.0 35.0 37.0 8 35.96857781474166 37.0 35.0 37.0 35.0 37.0 9 37.5722387841631 39.0 37.0 39.0 35.0 39.0 10-11 37.48499626302673 39.0 37.0 39.0 34.5 39.0 12-13 37.456579110038405 39.0 37.0 39.0 34.0 39.0 14-15 38.467295513103295 40.0 38.0 41.0 34.0 41.0 16-17 38.56712538247781 40.0 38.0 41.0 34.5 41.0 18-19 38.85022534229832 40.0 38.0 41.0 35.0 41.0 20-21 38.86332301216905 40.0 38.0 41.0 35.0 41.0 22-23 38.78752883510394 40.0 38.0 41.0 34.5 41.0 24-25 38.65030605249183 40.0 38.0 41.0 34.0 41.0 26-27 38.39311518868905 40.0 38.0 41.0 34.0 41.0 28-29 38.271332708631284 40.0 38.0 41.0 34.0 41.0 30-31 38.109024088023986 40.0 37.0 41.0 34.0 41.0 32-33 37.90703541414374 40.0 37.0 41.0 33.0 41.0 34-35 37.6653003065442 40.0 36.0 41.0 33.0 41.0 36-37 37.403340938401755 40.0 35.5 41.0 32.5 41.0 38-39 37.19388253260017 40.0 36.0 41.0 32.0 41.0 40-41 37.109975541088446 40.0 36.0 41.0 32.0 41.0 42-43 36.80763403230655 40.0 36.0 41.0 31.0 41.0 44-45 36.38549864710328 40.0 35.0 41.0 30.0 41.0 46-47 35.81779129425715 39.0 35.0 41.0 29.0 41.0 48-49 35.25657587882091 39.0 35.0 41.0 25.0 41.0 50-51 34.92543895387226 39.0 35.0 40.5 23.5 41.0 52-53 34.77194558629739 39.0 35.0 40.0 23.0 41.0 54-55 34.61374868995204 39.0 35.0 40.0 22.5 41.0 56-57 34.250172448672814 38.5 34.0 40.0 21.0 41.0 58-59 33.99225491215303 38.0 34.0 40.0 19.5 41.0 60-61 33.46032661989362 38.0 33.0 40.0 15.0 41.0 62-63 33.22929808121876 38.0 33.0 40.0 13.0 41.0 64-65 32.962960413371064 37.0 33.0 39.5 10.0 41.0 66-67 32.4370527222305 36.5 32.0 39.0 7.5 40.0 68-69 31.90773715029095 36.0 31.5 39.0 2.0 40.0 70-71 31.29441729909554 35.0 31.0 37.5 2.0 40.0 72-73 30.7145208806894 35.0 30.5 37.0 2.0 39.0 74-75 30.236731286333637 35.0 30.0 37.0 2.0 39.0 76-77 28.320070707468048 32.5 27.5 35.0 2.0 37.0 78-79 29.23051294875289 34.0 29.0 36.0 2.0 37.0 80-81 29.149559360275134 34.0 29.0 35.5 2.0 37.0 82-83 28.756695293380503 34.0 29.0 35.0 2.0 37.0 84-85 28.293384011935835 34.0 29.0 35.0 2.0 36.0 86-87 27.86273577351132 34.0 27.5 35.0 2.0 36.0 88-89 27.194593751668293 33.0 26.0 35.0 2.0 35.0 90-91 26.639470234867364 33.0 25.0 35.0 2.0 35.0 92-93 26.35244118481721 33.0 25.0 35.0 2.0 35.0 94-95 25.816101578239014 32.0 24.0 34.0 2.0 35.0 96-97 24.97552668845163 32.0 19.0 34.0 2.0 35.0 98-99 24.121135182900957 32.0 2.0 34.0 2.0 35.0 100-101 20.105261124519885 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1458799314523631 1101 2 0.22574306472152728 1101 3 0.14242405891892673 1101 4 0.12198667058504498 1101 5 0.17709325715416924 1101 6 0.23215742821656704 1101 7 0.16347777116291695 1101 8 0.22908778403812846 1101 9 0.3043557898140463 1101 10-11 0.22898116302703642 1101 12-13 0.21410302494192024 1101 14-15 0.3247569486187629 1101 16-17 0.2923849765957698 1101 18-19 0.3097824492695693 1101 20-21 0.37636509222494396 1101 22-23 0.23677926048586784 1101 24-25 0.27819524546293906 1101 26-27 0.386641511943985 1101 28-29 0.37005076659725233 1101 30-31 0.41718843284681384 1101 32-33 0.5796037271763765 1101 34-35 0.7556988445978234 1101 36-37 0.5500023160522716 1101 38-39 0.583806538196086 1101 40-41 0.644016320155572 1101 42-43 0.7504642302758313 1101 44-45 0.6997935246015388 1101 46-47 0.7935524100360354 1101 48-49 0.7033086048232065 1101 50-51 0.6722565810628396 1101 52-53 0.48372613245403073 1101 54-55 0.6066669040934016 1101 56-57 0.6417994989718707 1101 58-59 0.7263457523732129 1101 60-61 0.6474255027428484 1101 62-63 0.8634848939127764 1101 64-65 0.8550672919949633 1101 66-67 0.7118233755793213 1101 68-69 0.6996284123754926 1101 70-71 0.8149982974683354 1101 72-73 0.968148826300574 1101 74-75 0.6760185683394866 1101 76-77 0.7762686268783519 1101 78-79 0.7633916287578444 1101 80-81 0.8270121586760979 1101 82-83 0.8679135971279628 1101 84-85 0.9011029143128191 1101 86-87 0.8146057553970358 1101 88-89 0.8341160152617171 1101 90-91 0.737523117449463 1101 92-93 0.8277993574571418 1101 94-95 0.764180877779701 1101 96-97 0.6659713203555775 1101 98-99 0.6085118347101393 1101 100-101 0.3719960483353084 1102 1 0.23964413192968692 1102 2 0.23456852215987567 1102 3 0.30326733656411875 1102 4 0.19994307472187245 1102 5 0.31439222294573455 1102 6 0.2703518594019272 1102 7 0.3306841355225032 1102 8 0.3303084682067876 1102 9 0.31663009371221307 1102 10-11 0.33072881616943306 1102 12-13 0.2616390631280723 1102 14-15 0.30378574734589137 1102 16-17 0.432176862037295 1102 18-19 0.3496494341542231 1102 20-21 0.3790708997030663 1102 22-23 0.3982866590538805 1102 24-25 0.4539479900930061 1102 26-27 0.43198343716275645 1102 28-29 0.4473417769393393 1102 30-31 0.47545573594943846 1102 32-33 0.4328861456330131 1102 34-35 0.42778253592638293 1102 36-37 0.47998226831957425 1102 38-39 0.4751150505270303 1102 40-41 0.3884669168142878 1102 42-43 0.35428793751528787 1102 44-45 0.26616424854744025 1102 46-47 0.4460256001712821 1102 48-49 0.1580417790475579 1102 50-51 0.22896938933650546 1102 52-53 0.2204542151906992 1102 54-55 0.2715031809430428 1102 56-57 0.09917133668069766 1102 58-59 0.17328004036047417 1102 60-61 0.30529376049941703 1102 62-63 0.3352529130058528 1102 64-65 0.2772337199981436 1102 66-67 0.34704756014574656 1102 68-69 0.2618877600286389 1102 70-71 0.09492447089712996 1102 72-73 0.24367595438331335 1102 74-75 0.23756415306502632 1102 76-77 0.2860878790661019 1102 78-79 0.3961849461802771 1102 80-81 0.47257206321069845 1102 82-83 0.5106709559187337 1102 84-85 0.43110132322292216 1102 86-87 0.2892259622387243 1102 88-89 0.5331432228989499 1102 90-91 0.633826379183752 1102 92-93 0.6269777186345458 1102 94-95 0.8708647282172493 1102 96-97 0.6204979711113445 1102 98-99 0.34042209723997274 1102 100-101 0.1802678064896419 1103 1 0.08382742548577227 1103 2 0.20108117132455305 1103 3 0.19254339066117154 1103 4 0.13312429986109464 1103 5 0.2534655721899668 1103 6 0.2393173327511704 1103 7 0.17541094538726298 1103 8 0.16703527807154472 1103 9 0.18661513746718583 1103 10-11 0.09731847408510674 1103 12-13 0.22603619916625917 1103 14-15 0.2961173304803353 1103 16-17 0.2001017625941799 1103 18-19 0.1351603331200053 1103 20-21 0.2999927771891464 1103 22-23 0.29206963439198574 1103 24-25 0.24239572278990806 1103 26-27 0.4045412732804934 1103 28-29 0.3708463115455416 1103 30-31 0.44940800325254315 1103 32-33 0.38986625700930944 1103 34-35 0.48004252001548764 1103 36-37 0.42390844174837383 1103 38-39 0.5018654085222565 1103 40-41 0.5807704967665543 1103 42-43 0.6927872215248385 1103 44-45 0.6687672716182433 1103 46-47 0.5023829589620519 1103 48-49 0.6265385173132643 1103 50-51 0.75857320795226 1103 52-53 0.7247762517857694 1103 54-55 0.9304536980472591 1103 56-57 0.9301845427268418 1103 58-59 0.7343012018561055 1103 60-61 0.8662003635224877 1103 62-63 0.8332541858777702 1103 64-65 0.7842637915971906 1103 66-67 0.6302800183796435 1103 68-69 0.5870588021925194 1103 70-71 0.6348073666807394 1103 72-73 0.6332244030706633 1103 74-75 0.5626534132082206 1103 76-77 0.6139774574272785 1103 78-79 0.6106469986862457 1103 80-81 0.8142834395034626 1103 82-83 0.9319549654652732 1103 84-85 0.8159796048458325 1103 86-87 0.7756240529308478 1103 88-89 0.8500269142275094 1103 90-91 0.733147620233872 1103 92-93 0.7733045284993061 1103 94-95 0.7092882763477206 1103 96-97 0.6444916067517603 1103 98-99 0.4267298140259719 1103 100-101 0.14765237291764421 1104 1 0.1303093725713964 1104 2 0.15702597059379286 1104 3 0.17212906307290154 1104 4 0.07667768129177688 1104 5 0.09181193264780063 1104 6 0.06030999538359083 1104 7 0.11115716837201006 1104 8 0.14861801748104142 1104 9 0.20266095696661068 1104 10-11 0.11743500409915697 1104 12-13 0.09565464754365394 1104 14-15 0.08030950594024233 1104 16-17 0.17446807670498288 1104 18-19 0.21512414767599353 1104 20-21 0.20525546807586892 1104 22-23 0.19831141077275305 1104 24-25 0.2803311833703219 1104 26-27 0.3032381354056852 1104 28-29 0.2781686676956383 1104 30-31 0.2579202126492106 1104 32-33 0.2673307598423378 1104 34-35 0.2652001065910099 1104 36-37 0.3431162637359222 1104 38-39 0.21645378238341095 1104 40-41 0.14300259290290285 1104 42-43 0.26552032865356523 1104 44-45 0.35208021493399855 1104 46-47 0.3503834305761728 1104 48-49 0.24388960181301655 1104 50-51 0.24741400354582765 1104 52-53 0.29870753833814234 1104 54-55 0.18918813128682643 1104 56-57 0.08399425493890789 1104 58-59 0.24132679360723586 1104 60-61 0.5177805443038466 1104 62-63 0.3021598648775097 1104 64-65 0.18631057255735328 1104 66-67 0.2712938550273378 1104 68-69 0.2843323742379589 1104 70-71 0.3339111783073676 1104 72-73 0.26649398341310615 1104 74-75 0.10819547468458168 1104 76-77 0.2470476957200347 1104 78-79 0.4685574442704237 1104 80-81 0.49841958039939627 1104 82-83 0.6077664486612875 1104 84-85 0.7252984202435506 1104 86-87 0.7886520890530875 1104 88-89 0.7156211449768755 1104 90-91 0.849144637396126 1104 92-93 0.7613807744023084 1104 94-95 0.6363351636641568 1104 96-97 0.5104292163367106 1104 98-99 0.48015135621628957 1104 100-101 0.30666108380056656 1105 1 0.06085174807591898 1105 2 -6.503658827767822E-4 1105 3 0.0446077783005947 1105 4 0.03473302140885437 1105 5 0.07123670123971948 1105 6 0.11871926229606089 1105 7 0.17866159172966434 1105 8 0.09869414985187319 1105 9 0.0707358058218901 1105 10-11 0.1467446577535938 1105 12-13 0.10872573487216641 1105 14-15 0.10024210682799861 1105 16-17 0.16716117277606202 1105 18-19 0.1120140952357147 1105 20-21 -0.011340981485055579 1105 22-23 0.0013839934027046752 1105 24-25 -0.02723312186434157 1105 26-27 -0.055795085304872316 1105 28-29 -0.07694855055495253 1105 30-31 0.019493816680594023 1105 32-33 0.11477190343956778 1105 34-35 0.1834696646392544 1105 36-37 0.11336901622513551 1105 38-39 0.20560004671896337 1105 40-41 0.38468199678382575 1105 42-43 0.39460964353204986 1105 44-45 0.22248412668910333 1105 46-47 0.04166215767318704 1105 48-49 0.3131092581031467 1105 50-51 0.24070434744770353 1105 52-53 0.1678229889911549 1105 54-55 0.14751780394381342 1105 56-57 0.4569416337104002 1105 58-59 0.3399459961868345 1105 60-61 0.3875758625557779 1105 62-63 0.4101869571794978 1105 64-65 0.1915758372867984 1105 66-67 0.14614032480682937 1105 68-69 0.3396943099143961 1105 70-71 0.4073351335018529 1105 72-73 0.34075257281438454 1105 74-75 0.364171007596628 1105 76-77 0.4486277115108841 1105 78-79 0.36207161792437503 1105 80-81 0.4232809741017931 1105 82-83 0.6119550381731145 1105 84-85 0.5244966012122596 1105 86-87 0.5775897093826678 1105 88-89 0.5947618062957538 1105 90-91 0.5996460288410219 1105 92-93 0.4631507574768925 1105 94-95 0.9044409658410117 1105 96-97 0.7636358233581113 1105 98-99 0.6289972686032641 1105 100-101 0.4948310966648144 1106 1 0.05121985447303956 1106 2 0.1736124527957159 1106 3 0.08277600708434818 1106 4 0.06990376836349554 1106 5 0.018628061096023885 1106 6 0.12043509639615024 1106 7 0.08595812396180946 1106 8 0.08842060693510945 1106 9 0.1713514809954546 1106 10-11 0.20111783351047308 1106 12-13 0.20054542150957388 1106 14-15 0.2872030883863559 1106 16-17 0.13219073486966693 1106 18-19 0.23268874051260013 1106 20-21 0.2461992234024919 1106 22-23 0.22415544518104724 1106 24-25 0.22155319240514615 1106 26-27 0.32449962213384964 1106 28-29 0.2188897538179475 1106 30-31 0.3008141174523331 1106 32-33 0.3195826774249255 1106 34-35 0.2659397613599097 1106 36-37 0.17099556311725905 1106 38-39 0.39757343202992246 1106 40-41 0.41429581854839626 1106 42-43 0.5054174172840789 1106 44-45 0.6828289884318295 1106 46-47 0.5986082591308204 1106 48-49 0.23041518945218087 1106 50-51 0.26895782864286844 1106 52-53 0.32068080478607186 1106 54-55 0.36169797863090736 1106 56-57 0.411485787547754 1106 58-59 0.46351356637204333 1106 60-61 0.615521506164157 1106 62-63 0.41017430028909274 1106 64-65 0.5703418124836972 1106 66-67 0.6094452480070203 1106 68-69 0.6978559681211216 1106 70-71 0.5148042396832544 1106 72-73 0.426196763631868 1106 74-75 0.555574557689301 1106 76-77 0.6678555091239069 1106 78-79 0.7024462178565791 1106 80-81 0.6419755314187938 1106 82-83 0.7344773143002357 1106 84-85 0.7087776238009589 1106 86-87 0.7498392570364096 1106 88-89 0.9048148991995255 1106 90-91 0.877888228894733 1106 92-93 1.0266985278831022 1106 94-95 1.2692323582750547 1106 96-97 0.9885760057934299 1106 98-99 1.0516111145810108 1106 100-101 1.1103175174723 1107 1 0.061777845400818876 1107 2 0.08811492273007815 1107 3 0.05507061525263879 1107 4 -0.04714516476494168 1107 5 3.2294283169420623E-4 1107 6 0.08650182376401006 1107 7 0.03088826952107837 1107 8 0.10234154019538266 1107 9 0.08746744225106085 1107 10-11 0.1618854804602421 1107 12-13 0.17873728123213795 1107 14-15 0.13931190557825346 1107 16-17 0.19285141656331461 1107 18-19 0.2225993985163015 1107 20-21 0.17696484125688272 1107 22-23 0.18438131336606745 1107 24-25 0.20688113335744163 1107 26-27 0.17109305940081754 1107 28-29 0.11079922526435837 1107 30-31 0.20908510159448923 1107 32-33 0.1350928455617364 1107 34-35 0.3674213099819781 1107 36-37 0.2745373645419562 1107 38-39 0.26766729571591696 1107 40-41 0.3588891548755839 1107 42-43 0.45020696816390426 1107 44-45 0.40428826850467914 1107 46-47 0.36998483039224084 1107 48-49 0.22165660442174584 1107 50-51 0.40562512704142506 1107 52-53 0.32168814248934297 1107 54-55 0.45468436412194535 1107 56-57 0.6231801748845456 1107 58-59 0.4096766191202832 1107 60-61 0.33885641196057037 1107 62-63 0.4919174889146163 1107 64-65 0.43343272213642337 1107 66-67 0.4976482729041223 1107 68-69 0.4560859068568668 1107 70-71 0.41804008030553774 1107 72-73 0.5484155124731167 1107 74-75 0.29992965555967643 1107 76-77 0.32356385910886587 1107 78-79 0.5315129575844111 1107 80-81 0.5494250924337933 1107 82-83 0.6414378839301378 1107 84-85 0.5719908456748115 1107 86-87 0.640979062737653 1107 88-89 0.9442916191926081 1107 90-91 0.9887352886634382 1107 92-93 0.8372636772945619 1107 94-95 0.9310882492436221 1107 96-97 0.8052021763857589 1107 98-99 0.7319083410033365 1107 100-101 0.3341591821133072 1108 1 0.10167746526302324 1108 2 0.012962966604320059 1108 3 0.14521178100856247 1108 4 0.08334307472187419 1108 5 0.0852144451679564 1108 6 0.02065185940193004 1108 7 0.04795635774472373 1108 8 0.09999735709567403 1108 9 0.11559120482332474 1108 10-11 0.06263437172498953 1108 12-13 0.12026684090585604 1108 14-15 0.12665796956811448 1108 16-17 0.22806019537063094 1108 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-1.1651997477098064 2316 54-55 -1.0941417881407105 2316 56-57 -1.0284907206548795 2316 58-59 -1.0285023092741525 2316 60-61 -0.847398088854149 2316 62-63 -1.0844900886804894 2316 64-65 -0.7906079888270412 2316 66-67 -0.7684047164141141 2316 68-69 -0.8624629988246504 2316 70-71 -0.8916850850331706 2316 72-73 -0.7657266313390032 2316 74-75 -0.92636592000974 2316 76-77 -1.1676454542672339 2316 78-79 -1.1111879599467578 2316 80-81 -1.0491893757999797 2316 82-83 -0.9446758681397256 2316 84-85 -0.9781600595764033 2316 86-87 -1.0719023120726874 2316 88-89 -1.2429941576519212 2316 90-91 -1.3629248293603737 2316 92-93 -1.3244435292575254 2316 94-95 -1.164287154863132 2316 96-97 -0.9753311463703831 2316 98-99 -0.8576365874143299 2316 100-101 -0.34629048468517354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 9.0 5 44.0 6 112.0 7 272.0 8 578.0 9 1058.0 10 2011.0 11 3269.0 12 5329.0 13 7222.0 14 8972.0 15 12834.0 16 17644.0 17 26204.0 18 23517.0 19 12220.0 20 9666.0 21 9660.0 22 10262.0 23 11164.0 24 12624.0 25 14320.0 26 16708.0 27 19809.0 28 23730.0 29 28747.0 30 35002.0 31 42328.0 32 51805.0 33 66570.0 34 88692.0 35 127627.0 36 196166.0 37 305427.0 38 217563.0 39 14442.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.23584704273557 21.038140683090003 15.75831529171067 16.967696982463753 2 13.775663593731998 26.256170922976203 36.862220886027934 23.10594459726386 3 24.964175632124483 27.461063829189413 32.13333408260116 15.441426456084944 4 10.717175747324411 17.99696827506371 43.33596513656811 27.949890841043768 5 9.337235145531226 43.231231543426155 34.91470990691284 12.516823404129775 6 23.759352969769854 41.16205820125147 17.121589309446676 17.95699951953201 7 22.75191555002346 37.6657403843182 22.30326802527655 17.27907604038179 8 23.33845176916182 29.14565204564165 20.62570419468226 26.890191990514268 9 22.438979160052035 22.301441684953485 25.89160529694889 29.367973858045595 10-11 23.896152884353324 26.435503493929524 27.30463075613299 22.363712865584162 12-13 29.94551886328578 25.934840391904533 26.130223684543257 17.989417060266423 14-15 23.31635862470089 27.73673712593211 32.0433186977838 16.9035855515832 16-17 17.19387468389997 30.7578673784771 31.681336049452092 20.366921888170836 18-19 16.351476508864955 33.342980159966395 32.7162993555475 17.58924397562116 20-21 20.26043613006915 27.220619101271975 30.6084101567 21.910534611958877 22-23 18.03008825438392 27.817551411480096 32.17470771530445 21.977652618831538 24-25 22.054885741339636 28.260403817894204 31.439114028260512 18.245596412505655 26-27 20.623119658389825 24.81162339993158 37.22709363723658 17.338163304442013 28-29 16.58998378771744 32.12297311346069 33.63314582906016 17.653897269761703 30-31 18.291816871450926 28.14176194075352 32.402227573243266 21.16419361455228 32-33 19.0365422601102 28.744524491223732 33.498909815314846 18.720023433351223 34-35 21.852460121889198 26.757039255739723 32.562897411329686 18.827603211041396 36-37 20.250468390079895 25.759030778514543 36.09684065885818 17.89366017254739 38-39 18.93362977168276 28.926376866569374 34.675770555312624 17.46422280643524 40-41 18.27721964787414 29.898103583743072 33.67390126187741 18.150775506505376 42-43 16.986832432865288 30.70138868644992 30.33341610404243 21.978362776642363 44-45 19.478663464436064 27.03880538722147 31.006131676865056 22.47639947147741 46-47 22.245180296984532 29.899105056548166 30.3943953731694 17.46131927329791 48-49 20.821715255082598 28.79817646366713 30.99516545115139 19.384942830098886 50-51 16.42692420893067 30.160064792980666 34.71503100214876 18.697979995939903 52-53 19.016465874455875 32.680205477479454 28.91028518324177 19.393043464822902 54-55 16.997398167478217 28.749055220102104 31.20671222215042 23.046834390269257 56-57 20.66836495109032 28.24749480803333 29.660608874654177 21.42353136622217 58-59 23.554735396149752 27.608413065307925 29.126601649442968 19.710249889099355 60-61 19.636175764321358 29.409851576935786 33.212466006064204 17.741506652678652 62-63 18.29803524891296 29.382301083879486 32.38906567199826 19.930597995209297 64-65 17.430205702115465 33.178141235111816 30.27777231436655 19.113880748406167 66-67 19.04394795043146 29.104045400249856 28.281977192401403 23.570029456917283 68-69 17.21367239786075 29.87917463218701 30.151796505087336 22.7553564648649 70-71 21.263026723573557 29.556086911321344 28.430604687232197 20.750281677872902 72-73 19.803183732646254 26.440034222807903 33.00021354133008 20.756568503215764 74-75 17.074736655774185 33.47709909722593 28.97601312717229 20.472151119827593 76-77 18.61594280977744 28.451727263316016 29.390448906063043 23.5418810208435 78-79 18.705959249387387 29.109261276170695 30.690241467030482 21.49453800741144 80-81 20.817892828054323 28.553757192544726 29.249664321571327 21.37868565782962 82-83 18.85548871462168 27.8872333191909 32.16287162513382 21.094406341053602 84-85 17.947060013543016 29.440746495533897 30.76916322705906 21.84303026386403 86-87 18.00428317624486 30.253703126170166 29.85252892691 21.889484770674976 88-89 17.81842832758369 29.684936500113864 28.003016182899977 24.493618989402467 90-91 18.773655441187294 26.82637526884485 29.62958136802628 24.770387921941577 92-93 20.49139793707836 29.77886530880605 29.540949359797473 20.188787394318116 94-95 19.160628036290785 28.40925055422404 29.811458459186913 22.618662950298255 96-97 15.575486860183815 28.872895142777665 32.30841690011042 23.243201096928097 98-99 18.285527035328155 30.17396975785846 27.70208403357803 23.838419173235355 100-101 17.45968860744378 27.666171445028642 29.68541133249743 25.188728615030154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2569.0 1 1550.0 2 629.5 3 707.0 4 563.5 5 610.5 6 1194.0 7 2358.5 8 3415.5 9 3668.5 10 3295.0 11 2955.5 12 3016.0 13 3212.5 14 3481.5 15 3792.5 16 4035.0 17 4290.0 18 4751.0 19 5296.5 20 5908.5 21 6659.0 22 7449.5 23 8174.0 24 9068.0 25 10743.0 26 13984.0 27 17519.5 28 18676.5 29 20035.0 30 23298.0 31 26288.5 32 30292.5 33 35469.0 34 41679.0 35 45863.5 36 49722.5 37 54294.0 38 52026.0 39 46369.0 40 42034.5 41 38562.5 42 35347.5 43 33528.0 44 33131.5 45 33581.0 46 40556.5 47 85224.5 48 178334.0 49 168774.0 50 73210.0 51 29925.0 52 14022.0 53 8364.0 54 6187.0 55 5021.5 56 4085.0 57 3227.5 58 2484.5 59 1883.5 60 1435.5 61 1221.5 62 1003.5 63 799.0 64 689.0 65 576.0 66 478.0 67 437.0 68 387.5 69 325.0 70 282.5 71 241.0 72 174.0 73 120.0 74 96.0 75 77.0 76 51.0 77 31.0 78 22.0 79 15.0 80 16.0 81 11.0 82 6.0 83 4.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29565640076088145 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0016507306766169432 16-17 8.429263029533328E-4 18-19 3.51219292897222E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.02438585794444E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016858526059066656 36-37 0.008359019170953884 38-39 0.003125851706785276 40-41 0.004109265726897498 42-43 3.51219292897222E-5 44-45 0.0014751210301683325 46-47 1.75609646448611E-4 48-49 3.160973636074998E-4 50-51 7.02438585794444E-5 52-53 7.02438585794444E-5 54-55 0.0 56-57 0.0012995113837197213 58-59 0.0028448762724674983 60-61 8.78048232243055E-4 62-63 0.001545364888747777 64-65 0.0 66-67 5.26828939345833E-4 68-69 0.0020019499695141654 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2563198399563926 78-79 1.053657878691666E-4 80-81 1.75609646448611E-4 82-83 0.0 84-85 0.0 86-87 0.10792968870731631 88-89 0.36969342770361585 90-91 0.272581293217534 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.75609646448611E-4 100-101 4.917070100561108E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1423612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.83683639013962 #Duplication Level Percentage of deduplicated Percentage of total 1 73.14324306917702 17.435035180841865 2 12.801173120265785 6.1027893853925725 3 4.897018179913671 3.501882634624244 4 2.393187615514984 2.2818408656775615 5 1.4658158861988586 1.7470206728694853 6 0.9447308376883204 1.3511636646417629 7 0.6269560502220917 1.046125415506651 8 0.46287223788227205 0.8826727843150004 9 0.32408618898623215 0.6952670516851817 >10 2.238881361749657 10.624070978091032 >50 0.3667923610951952 6.095969479795029 >100 0.292784863383453 13.94646285581127 >500 0.022408509181322596 3.6342443724847304 >1k 0.012088801005713506 5.473470444997309 >5k 0.004422732075261039 7.682593984728111 >10k+ 0.0035381856602088313 17.499390228538225 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 60122 4.223201265513357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32741 2.2998541737495892 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24147 1.6961784531178439 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19084 1.340533797130117 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15847 1.1131544269084555 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15657 1.099808093778361 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15335 1.0771895713157797 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14248 1.0008344970399237 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 14083 0.9892442603743155 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13134 0.9225828385824227 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12341 0.8668794587289234 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12247 0.8602765360224555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9965 0.6999800507441635 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9620 0.6757459195342551 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9235 0.6487020339811691 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8162 0.5733303737254252 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7991 0.5613186739083402 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7932 0.557174286252153 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7827 0.5497986811013114 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7719 0.5422123443747313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 0.4912153030460547 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6875 0.4829265277336802 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5653 0.3970885325495992 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5557 0.39034512212597255 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5433 0.38163488366212145 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5281 0.3709578171580459 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5067 0.35592563142204475 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4161 0.29228469554906816 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3914 0.2749344624799454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3526 0.24767984535112095 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3243 0.22780083337313817 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3238 0.22744961408024097 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2784 0.1955589022851732 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2761 0.19394329353784598 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2556 0.17954330252905987 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2460 0.17279989210543323 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2393 0.16809355358061046 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2390 0.16788282200487212 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2342 0.16451111679305877 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2319 0.16289550804573155 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2265 0.15910233968244156 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2176 0.15285063626887102 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2032 0.14273552063343103 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1984 0.1393638154216177 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1800 0.1264389454429999 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1733 0.12173260691817714 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1705 0.11976577887795269 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1608 0.11295212459574658 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1596 0.11210919829279327 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1507 0.10585749487922272 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.75609646448611E-4 0.0 12-13 0.0 0.0 0.0 2.458535050280554E-4 0.0 14-15 0.0 0.0 0.0 3.51219292897222E-4 0.0 16-17 0.0 0.0 0.0 6.673166565047217E-4 0.0 18-19 0.0 0.0 0.0 8.078043736636105E-4 0.0 20-21 0.0 0.0 0.0 0.0011590236665608325 0.0 22-23 0.0 0.0 0.0 0.002282925403831943 0.0 24-25 0.0 0.0 0.0 0.004355119231925553 0.0 26-27 0.0 0.0 0.0 0.007937556019477217 0.0 28-29 0.0 0.0 0.0 0.019106329533608878 0.0 30-31 0.0 0.0 0.0 0.050399968530751356 0.0 32-33 0.0 0.0 0.0 0.11354919739367186 0.0 34-35 0.0 0.0 0.0 0.2105559660918846 0.0 36-37 0.0 0.0 0.0 0.33502808349466007 0.0 38-39 0.0 0.0 0.0 0.51330699656929 0.0 40-41 0.0 0.0 0.0 0.7061263883698647 0.0 42-43 0.0 0.0 0.0 0.9077965063514497 0.0 44-45 0.0 0.0 0.0 1.1353514862195597 0.0 46-47 0.0 0.0 0.0 1.4344498360508342 0.0 48-49 0.0 0.0 0.0 1.7160574650958267 0.0 50-51 0.0 0.0 0.0 2.027027027027027 0.0 52-53 0.0 0.0 0.0 2.322613183929329 0.0 54-55 0.0 0.0 0.0 2.654164196424307 0.0 56-57 0.0 0.0 0.0 3.1051648904336293 0.0 58-59 0.0 0.0 0.0 3.5278924313647257 0.0 60-61 0.0 0.0 0.0 4.026623827278781 0.0 62-63 0.0 0.0 0.0 4.49838158149833 0.0 64-65 0.0 0.0 0.0 4.960586170951073 0.0 66-67 0.0 0.0 0.0 5.518778993152629 0.0 68-69 0.0 0.0 0.0 6.178649800647929 0.0 70-71 0.0 0.0 0.0 6.868233760322335 0.0 72-73 0.0 0.0 0.0 7.507804092688176 0.0 74-75 0.0 0.0 0.0 8.242133390277688 0.0 76-77 0.0 0.0 0.0 8.90432224510611 0.0 78-79 0.0 0.0 0.0 9.85387872538304 0.0 80-81 0.0 0.0 0.0 10.859489804806365 0.0 82-83 0.0 0.0 0.0 11.710564395354915 0.0 84-85 0.0 0.0 0.0 12.669322821105752 0.0 86-87 0.0 0.0 0.0 13.626606125826418 0.0 88-89 0.0 0.0 0.0 14.624911843957483 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 425 0.0 41.868702 94-95 GCTTATG 390 0.0 37.829224 1 TCACGCA 55 0.0025928684 34.509964 4 CATATAG 455 0.0 31.286505 3 GTCCTAT 110 2.693294E-6 30.285568 1 CCTCGTC 95 3.516006E-5 29.969177 8 ATCACGC 65 0.005873965 29.200737 3 CATTCCG 280 0.0 28.81269 9 GACATAT 480 0.0 28.75326 1 CTTATGT 470 0.0 27.2592 2 TTAGGCA 410 0.0 26.618963 4 GGACAAT 540 0.0 26.361776 6 CATGGGG 3490 0.0 25.017252 4 TAGGCAT 365 0.0 24.700623 5 GTACATG 10280 0.0 24.490232 1 GATTGGC 740 0.0 24.369398 9 GAGTACT 10600 0.0 23.840023 12-13 TACATGG 10520 0.0 23.680492 2 AGTACTT 10795 0.0 23.541264 12-13 GGATATG 810 0.0 23.502028 1 >>END_MODULE