##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449471 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1104499503612 33.0 31.0 34.0 30.0 34.0 2 32.27843261438139 34.0 31.0 34.0 30.0 34.0 3 32.370142624447126 34.0 31.0 34.0 30.0 34.0 4 35.89345423261314 37.0 35.0 37.0 35.0 37.0 5 35.861571566454245 37.0 35.0 37.0 35.0 37.0 6 35.964568452904544 37.0 35.0 37.0 35.0 37.0 7 35.927006473396155 37.0 35.0 37.0 35.0 37.0 8 35.944848844854434 37.0 35.0 37.0 35.0 37.0 9 37.55018279082507 39.0 37.0 39.0 35.0 39.0 10-11 37.45140502983502 39.0 37.0 39.0 34.5 39.0 12-13 37.42551765437184 39.0 37.0 39.0 34.0 39.0 14-15 38.43853240251099 40.0 38.0 41.0 34.0 41.0 16-17 38.47353379267332 40.0 38.0 41.0 34.0 41.0 18-19 38.770688754725 40.0 38.0 41.0 34.0 41.0 20-21 38.802480353177124 40.0 38.0 41.0 34.0 41.0 22-23 38.75336105379135 40.0 38.0 41.0 34.0 41.0 24-25 38.614239263841775 40.0 38.0 41.0 34.0 41.0 26-27 38.34989040829378 40.0 38.0 41.0 34.0 41.0 28-29 38.23864947970674 40.0 38.0 41.0 34.0 41.0 30-31 38.085784744917284 40.0 37.0 41.0 34.0 41.0 32-33 37.7739847847939 40.0 36.5 41.0 33.0 41.0 34-35 37.54899960054392 40.0 36.0 41.0 33.0 41.0 36-37 37.35094941533842 40.0 35.5 41.0 32.5 41.0 38-39 37.16464730925972 40.0 36.0 41.0 32.0 41.0 40-41 37.09348134595311 40.0 36.0 41.0 32.0 41.0 42-43 36.799115677374715 40.0 35.5 41.0 31.0 41.0 44-45 36.390561798062876 40.0 35.0 41.0 30.0 41.0 46-47 35.855136460129245 40.0 35.0 41.0 29.0 41.0 48-49 35.30063278258068 39.0 35.0 41.0 25.5 41.0 50-51 34.98339463155868 39.0 35.0 41.0 24.0 41.0 52-53 34.86060673169729 39.0 35.0 41.0 23.0 41.0 54-55 34.66722859581185 39.0 35.0 40.5 22.5 41.0 56-57 34.37619276273895 38.5 34.5 40.0 21.0 41.0 58-59 34.13872854303398 38.0 34.0 40.0 20.0 41.0 60-61 33.61753460400381 38.0 34.0 40.0 18.0 41.0 62-63 33.36218040926656 38.0 33.0 40.0 13.5 41.0 64-65 32.96264533750589 37.0 33.0 39.5 10.5 41.0 66-67 32.44051347008667 36.5 32.0 39.0 7.5 40.0 68-69 32.00380863087292 36.0 32.0 39.0 4.0 40.0 70-71 31.494923665254426 35.5 31.5 38.0 2.0 40.0 72-73 30.916677532699858 35.0 31.0 37.0 2.0 39.0 74-75 30.451819663863574 35.0 30.5 37.0 2.0 39.0 76-77 28.498238667762237 32.5 27.5 35.0 2.0 37.0 78-79 29.42737522861789 34.0 29.0 36.0 2.0 37.5 80-81 29.381607496804005 34.0 29.5 35.5 2.0 37.0 82-83 29.039588235984024 34.0 29.5 35.0 2.0 37.0 84-85 28.63283018425343 34.0 29.0 35.0 2.0 36.0 86-87 28.267387205401143 34.0 29.0 35.0 2.0 36.0 88-89 27.691146977069565 34.0 27.5 35.0 2.0 35.5 90-91 27.19595252336887 33.0 26.0 35.0 2.0 35.0 92-93 26.92242825141034 33.0 26.0 35.0 2.0 35.0 94-95 26.494821559037746 33.0 25.0 35.0 2.0 35.0 96-97 25.843255228976638 33.0 24.0 35.0 2.0 35.0 98-99 25.215743191826533 32.5 19.0 34.0 2.0 35.0 100-101 22.080648733227502 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25351802639617205 1101 2 0.20610347364653592 1101 3 0.2215226321418413 1101 4 0.16837410749304382 1101 5 0.200510196152635 1101 6 0.1391615158033943 1101 7 0.2656217041127533 1101 8 0.3289040172956845 1101 9 0.3010261015986444 1101 10-11 0.3053699557528873 1101 12-13 0.41989264297838247 1101 14-15 0.3973435990703962 1101 16-17 0.3752836908358148 1101 18-19 0.372404164989085 1101 20-21 0.3825723641006178 1101 22-23 0.37595165834398614 1101 24-25 0.3422445333087083 1101 26-27 0.43020005357711 1101 28-29 0.3994580899940203 1101 30-31 0.5315555157493534 1101 32-33 0.575294227655121 1101 34-35 0.5349122032451703 1101 36-37 0.6568685039439046 1101 38-39 0.6449552414421973 1101 40-41 0.750171531684849 1101 42-43 0.7522259235568143 1101 44-45 0.8229666608891932 1101 46-47 0.7310099080804378 1101 48-49 0.7542744780328832 1101 50-51 0.7685943960824133 1101 52-53 0.9066280982928205 1101 54-55 0.8692833214742564 1101 56-57 0.9786781666924185 1101 58-59 0.7963714451755166 1101 60-61 0.8609804872101563 1101 62-63 1.0508528522597231 1101 64-65 0.9247723181104774 1101 66-67 0.8662010955298882 1101 68-69 1.0426758704454286 1101 70-71 1.0313022368509515 1101 72-73 1.0182626798211167 1101 74-75 0.7236656317000936 1101 76-77 1.056260933629325 1101 78-79 1.1301794420348976 1101 80-81 1.1609982443416662 1101 82-83 1.0758860940827937 1101 84-85 0.9318115025316196 1101 86-87 1.0662368036879215 1101 88-89 1.398935292048197 1101 90-91 1.4125145465738065 1101 92-93 1.2272845994130002 1101 94-95 1.4896754027632646 1101 96-97 1.4930456495277475 1101 98-99 1.455412354248434 1101 100-101 1.1408713123984846 1102 1 0.09382578937274388 1102 2 0.18458303267271958 1102 3 0.2415629526644807 1102 4 0.07289868259639576 1102 5 0.11531140066444578 1102 6 0.09311445818077146 1102 7 0.11687470263262867 1102 8 0.11082102847314701 1102 9 0.34614680797336916 1102 10-11 0.2488561498017816 1102 12-13 0.30044819853394245 1102 14-15 0.28545925262013583 1102 16-17 0.33619064740192783 1102 18-19 0.40660515513862805 1102 20-21 0.3236495345186228 1102 22-23 0.22894635032582045 1102 24-25 0.30525810958595656 1102 26-27 0.33080741334845243 1102 28-29 0.38989089762940665 1102 30-31 0.39716695861159934 1102 32-33 0.3669238913105062 1102 34-35 0.48867630266843065 1102 36-37 0.3850864899593134 1102 38-39 0.3366525312906177 1102 40-41 0.4128227336447239 1102 42-43 0.4380831174525994 1102 44-45 0.45983441978267336 1102 46-47 0.4889298284601651 1102 48-49 0.2906407823252408 1102 50-51 0.20232710674438437 1102 52-53 0.2685545516179886 1102 54-55 0.28659179899173637 1102 56-57 0.33490360434872457 1102 58-59 0.21379093172684094 1102 60-61 0.028637303420026683 1102 62-63 0.27065456715789793 1102 64-65 0.414355332452331 1102 66-67 0.2701489340436396 1102 68-69 0.4536606609318241 1102 70-71 0.43217372156487954 1102 72-73 0.4622643640961144 1102 74-75 0.5430590375082893 1102 76-77 0.6019162697187426 1102 78-79 0.5918188030311704 1102 80-81 0.6711421732958875 1102 82-83 0.43952744558588464 1102 84-85 0.4277807262339621 1102 86-87 0.39221164164024813 1102 88-89 0.8481441931863571 1102 90-91 0.8014251268830996 1102 92-93 0.8322800059357327 1102 94-95 0.7286829564814283 1102 96-97 0.6568043898942015 1102 98-99 0.5728752236405619 1102 100-101 0.7003083562406722 1103 1 0.10004375237697616 1103 2 0.11852553322988513 1103 3 0.04667525239456438 1103 4 0.026341963184762562 1103 5 0.06696308577947008 1103 6 0.04103685126697343 1103 7 0.14695914410553002 1103 8 0.05716973956444349 1103 9 0.19369280505134867 1103 10-11 0.16326382134673878 1103 12-13 0.15292696435123787 1103 14-15 0.13978594949752932 1103 16-17 0.17414411792170625 1103 18-19 0.2059427476899529 1103 20-21 0.30270569676511627 1103 22-23 0.2148940263281034 1103 24-25 0.2172489225518035 1103 26-27 0.2293946901728603 1103 28-29 0.1839707084408886 1103 30-31 0.20494065051165933 1103 32-33 0.24140325143729768 1103 34-35 0.18049649928582312 1103 36-37 0.3146335766046846 1103 38-39 0.2528660369736997 1103 40-41 0.27936125232833575 1103 42-43 0.2589947634422458 1103 44-45 0.2334204584623194 1103 46-47 0.27080153189973544 1103 48-49 0.32175783653121925 1103 50-51 0.37049192857535473 1103 52-53 0.306785985637255 1103 54-55 0.17972257184170104 1103 56-57 0.29162053969280066 1103 58-59 0.33020749315119957 1103 60-61 0.2511622520370196 1103 62-63 0.27282565400137315 1103 64-65 0.25868586757849954 1103 66-67 0.25324392450992406 1103 68-69 0.0992745331057634 1103 70-71 0.22215279691836898 1103 72-73 0.24865467685148346 1103 74-75 0.21808627669309288 1103 76-77 0.3494053121375842 1103 78-79 0.30331188180194246 1103 80-81 0.3191345215612422 1103 82-83 0.23550718953754668 1103 84-85 0.23397640263244313 1103 86-87 0.32358251552282624 1103 88-89 0.5450995952119619 1103 90-91 0.4138814822801713 1103 92-93 0.2893058583984036 1103 94-95 0.24224373700788604 1103 96-97 0.08780815385428653 1103 98-99 0.109181961624369 1103 100-101 0.2527982653608447 1104 1 0.21996842571630992 1104 2 0.19809594943138364 1104 3 0.2528470232221238 1104 4 0.20851360862862833 1104 5 0.1446985599588686 1104 6 0.1358305348226594 1104 7 0.18085252457026968 1104 8 0.13806030130746905 1104 9 0.19576163587258577 1104 10-11 0.2290866397308804 1104 12-13 0.29875663061362445 1104 14-15 0.20942272260369776 1104 16-17 0.2709144029525845 1104 18-19 0.29737819799822063 1104 20-21 0.30552743250935066 1104 22-23 0.3044517353752525 1104 24-25 0.21641644721260178 1104 26-27 0.30782098023837534 1104 28-29 0.35885081029101684 1104 30-31 0.4145984494974897 1104 32-33 0.46365728843030496 1104 34-35 0.33935280717027894 1104 36-37 0.27359634006112543 1104 38-39 0.35505615239902255 1104 40-41 0.4866488174012602 1104 42-43 0.5345386754614836 1104 44-45 0.4657733074448984 1104 46-47 0.42894050042600895 1104 48-49 0.43260123471845446 1104 50-51 0.4373618212999659 1104 52-53 0.33313343829888 1104 54-55 0.2044569151567046 1104 56-57 0.3509348597153661 1104 58-59 0.46703545467769203 1104 60-61 0.5500444082506277 1104 62-63 0.5573495697338942 1104 64-65 0.36892465116384443 1104 66-67 0.3949256814247164 1104 68-69 0.3786999141395526 1104 70-71 0.39499541386879855 1104 72-73 0.3759281603719664 1104 74-75 0.3888889975190821 1104 76-77 0.3768408299601518 1104 78-79 0.5542172966893268 1104 80-81 0.4852383681880603 1104 82-83 0.39516764087059286 1104 84-85 0.4062638350880121 1104 86-87 0.25544733261937225 1104 88-89 0.49795774044967445 1104 90-91 0.7882084491764694 1104 92-93 0.6978077162470626 1104 94-95 0.6301543384396702 1104 96-97 0.630304181361538 1104 98-99 0.6183837980131237 1104 100-101 0.7509241409368279 1105 1 0.022752476852559766 1105 2 0.10052662186423333 1105 3 0.13970352514566997 1105 4 0.10903820245859919 1105 5 0.09199050737370129 1105 6 0.15990373103635847 1105 7 0.25526180326539816 1105 8 0.19038108158255795 1105 9 0.22461895543572297 1105 10-11 0.12494824200162924 1105 12-13 0.24421442142600824 1105 14-15 0.15582772907376352 1105 16-17 0.18126756035751868 1105 18-19 0.15418353338768753 1105 20-21 0.19047319691500775 1105 22-23 0.20930154942630708 1105 24-25 0.31546701857029547 1105 26-27 0.17386610881860065 1105 28-29 0.24395992790416443 1105 30-31 0.2865076961625874 1105 32-33 0.3379463671182137 1105 34-35 0.4013708278535404 1105 36-37 0.29145483483556944 1105 38-39 0.2750043452641293 1105 40-41 0.3793898201628352 1105 42-43 0.205548518584159 1105 44-45 0.20459016433118649 1105 46-47 0.2641092521512718 1105 48-49 0.40332723344424437 1105 50-51 0.3722712461685518 1105 52-53 0.47038885982004075 1105 54-55 0.32499265544082334 1105 56-57 0.40442968657261247 1105 58-59 0.3447762070225835 1105 60-61 0.2740150500976952 1105 62-63 0.28961337147842414 1105 64-65 0.33361706377133515 1105 66-67 0.4210176437480726 1105 68-69 0.4355870134874138 1105 70-71 0.5310968803787652 1105 72-73 0.33868450261120486 1105 74-75 0.482457190640428 1105 76-77 0.5805702331880802 1105 78-79 0.4454697761056572 1105 80-81 0.42549768339744176 1105 82-83 0.5052904149283535 1105 84-85 0.47787223231642173 1105 86-87 0.4628997913035313 1105 88-89 0.6984486153822651 1105 90-91 0.8299866871515142 1105 92-93 0.8114462240431735 1105 94-95 0.7372220289805611 1105 96-97 0.40211634788513706 1105 98-99 0.44680585844158216 1105 100-101 0.2989782286106184 1106 1 0.04967757902321068 1106 2 0.10739189170494257 1106 3 0.12726032737728588 1106 4 0.07248931973295214 1106 5 0.11463554374318363 1106 6 0.04161112846289683 1106 7 0.0708989378471685 1106 8 0.031338155235680176 1106 9 0.1838647557941826 1106 10-11 0.2139498017617072 1106 12-13 0.16171850631416618 1106 14-15 0.2697264814318103 1106 16-17 0.1811205542164842 1106 18-19 0.05149628327736622 1106 20-21 0.1839177196766002 1106 22-23 0.2286783984230425 1106 24-25 0.23184599431123587 1106 26-27 0.3113323541599513 1106 28-29 0.2368297147923215 1106 30-31 0.2588212627530453 1106 32-33 0.300590196431628 1106 34-35 0.25055803062290494 1106 36-37 0.41777020758058114 1106 38-39 0.4953646127433231 1106 40-41 0.30530287779644283 1106 42-43 0.4467091860529493 1106 44-45 0.3863412483284421 1106 46-47 0.28763438743290237 1106 48-49 0.02666704685995569 1106 50-51 0.2006010183173359 1106 52-53 0.25341803901637405 1106 54-55 0.25365674890018397 1106 56-57 0.3529091558223101 1106 58-59 0.3541405812609071 1106 60-61 0.43004587321580345 1106 62-63 0.508451530175293 1106 64-65 0.41257209650304105 1106 66-67 0.45065142404282454 1106 68-69 0.4566122138282722 1106 70-71 0.47637820472968073 1106 72-73 0.5363205552720736 1106 74-75 0.5589189327767059 1106 76-77 0.6433756308327716 1106 78-79 0.7895693482651858 1106 80-81 0.5947059044459522 1106 82-83 0.6113612979120191 1106 84-85 0.48004271846656366 1106 86-87 0.4953197670848901 1106 88-89 0.6535907019392582 1106 90-91 0.8475667944244698 1106 92-93 0.7109327822556679 1106 94-95 0.8560417496190595 1106 96-97 0.7965822726368188 1106 98-99 0.6680949500322235 1106 100-101 0.41517370968060163 1107 1 -0.08021306129499806 1107 2 -0.027760148225702608 1107 3 -0.044317707437144804 1107 4 -0.10190908753715178 1107 5 0.04528352171305983 1107 6 -0.0033332238193182206 1107 7 -0.031065852248431725 1107 8 -0.046618604603878566 1107 9 -0.05347322889878825 1107 10-11 0.010449521805917072 1107 12-13 -0.017784410845976595 1107 14-15 0.026075962004547648 1107 16-17 -0.056869966834319996 1107 18-19 0.03244602028544108 1107 20-21 -0.01990570627457089 1107 22-23 0.015731952196411214 1107 24-25 0.005077121232162085 1107 26-27 -0.11297603015169955 1107 28-29 -0.18203904526371417 1107 30-31 -0.09430185743228492 1107 32-33 -0.11007372548881023 1107 34-35 0.01741860223226155 1107 36-37 0.0028840527993665432 1107 38-39 -0.08315726231521836 1107 40-41 -0.01921557743040836 1107 42-43 -0.15188832910525463 1107 44-45 -0.1662256548609662 1107 46-47 -0.17477582377239997 1107 48-49 -0.14028911560183133 1107 50-51 -0.2048236194573363 1107 52-53 -0.17231171934744083 1107 54-55 -0.3376988615595451 1107 56-57 -0.36578887283101125 1107 58-59 -0.20578166596190783 1107 60-61 -0.3910735648744179 1107 62-63 -0.2942415612651388 1107 64-65 -0.30066445260994357 1107 66-67 -0.24659642321162778 1107 68-69 -0.14423717620113763 1107 70-71 -0.17069511246542746 1107 72-73 -0.16842833791386624 1107 74-75 -0.030707768553135395 1107 76-77 0.07982332489208943 1107 78-79 0.04093634428752324 1107 80-81 0.0025026211577490187 1107 82-83 0.25309932381781763 1107 84-85 0.04471718920441958 1107 86-87 0.09024325277384548 1107 88-89 0.283874217018365 1107 90-91 0.20751240598737652 1107 92-93 0.3440262625120205 1107 94-95 0.3411767736494653 1107 96-97 0.32929078766388997 1107 98-99 0.46181020273134976 1107 100-101 0.504435115537401 1108 1 -0.005465415578839838 1108 2 0.07762486156360637 1108 3 0.041967265332942816 1108 4 0.07390696851034306 1108 5 0.10352643510589843 1108 6 0.060474945352581244 1108 7 0.0657715779446022 1108 8 0.021482903285964028 1108 9 -0.007397167268713645 1108 10-11 0.14567905086996547 1108 12-13 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-0.7674509196823536 2316 66-67 -0.6429094527689898 2316 68-69 -0.4389946986827624 2316 70-71 -0.3906898028558352 2316 72-73 -0.3514362237267825 2316 74-75 -0.41002523508334576 2316 76-77 -0.7038810662031665 2316 78-79 -0.5615872441744223 2316 80-81 -0.6158326959270006 2316 82-83 -0.6262943380161978 2316 84-85 -0.6543338183381167 2316 86-87 -0.7870626577359765 2316 88-89 -0.9562572880554434 2316 90-91 -1.2343120785313424 2316 92-93 -0.8155884619497193 2316 94-95 -0.5375383489527898 2316 96-97 -0.6412833880633357 2316 98-99 -0.7433532722878198 2316 100-101 -0.2347327194572557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 16.0 5 39.0 6 114.0 7 257.0 8 536.0 9 1109.0 10 2090.0 11 3366.0 12 5136.0 13 7594.0 14 9080.0 15 12810.0 16 17631.0 17 26429.0 18 23613.0 19 12351.0 20 9718.0 21 9485.0 22 9980.0 23 10830.0 24 12135.0 25 13993.0 26 16029.0 27 19229.0 28 23362.0 29 28403.0 30 34355.0 31 41025.0 32 50696.0 33 64421.0 34 85500.0 35 121206.0 36 186113.0 37 309411.0 38 258270.0 39 23136.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.25457231872589 21.109586236283043 15.70394340008965 16.931898044901413 2 13.713899760671309 26.285865670993076 36.884697934625805 23.115536633709816 3 25.037686162744894 27.47485116984058 31.99815656884477 15.489306098569756 4 10.719221012355543 18.009052957941208 43.31449197672806 27.95723405297519 5 9.32871371693535 43.342916139750294 34.79959240302152 12.528777740292837 6 23.810617804702545 41.20482576057058 17.017518805136493 17.967037629590383 7 22.76175239104473 37.660843162781454 22.37678435787953 17.20062008829428 8 23.394948915845852 29.184095438956696 20.581370720766405 26.839584924431055 9 22.47337131960557 22.292822691864824 25.96906043653167 29.264745551997933 10-11 23.892751217513148 26.454651386609324 27.284505864553342 22.368091531324186 12-13 29.91801836670068 25.953296064564245 26.15612868418892 17.972556884546158 14-15 23.328933914893675 27.79136545713706 31.93429297229125 16.94540765567801 16-17 17.150540768676983 30.75797992982926 31.748897610117393 20.342581691376367 18-19 16.401087017263542 33.313153557401286 32.67095374795356 17.61480567738161 20-21 20.199438277826875 27.289749156761328 30.61016743349815 21.90064513191364 22-23 18.015124138392558 27.886725570915182 32.14341646021204 21.954733830480226 24-25 22.030286232110278 28.346075342685484 31.378182584605895 18.245455840598343 26-27 20.628402576696175 24.835955356951896 37.20820888983638 17.327433176515555 28-29 16.61444071664766 32.22161740386665 33.47983505706565 17.68410682242004 30-31 18.35379942061621 28.104046234798762 32.38916128711785 21.152993057467175 32-33 19.045638029322422 28.794539525109506 33.467002789293474 18.6928196562746 34-35 21.845945274661588 26.837838583709345 32.54067144099019 18.77554470063888 36-37 20.200943995142012 25.840107372546857 36.068274034613154 17.890674597697977 38-39 18.967174341120252 28.914088621551393 34.621838419047386 17.496898618280973 40-41 18.293967483540108 29.944497136187476 33.57683473933458 18.18470064093784 42-43 16.96317533758039 30.679617813081382 30.340855894750607 22.01635095458762 44-45 19.45630134236106 27.063508435204536 31.021600443199233 22.458589779235176 46-47 22.25318375211888 29.979357957983233 30.34307736143374 17.42438092846415 48-49 20.79372405518979 28.84714492390672 30.944737769848444 19.41439325105504 50-51 16.440101250732162 30.191980384567884 34.67547815720356 18.692440207496393 52-53 19.03366814513709 32.66884953200167 28.93679832159457 19.36068400126667 54-55 17.03807802984675 28.766494810865485 31.18672260431564 23.008704554972123 56-57 20.651403391790314 28.282500402733273 29.677837527566105 21.38825867791031 58-59 23.608881358130564 27.601083987656118 29.167595171453154 19.622439482760168 60-61 19.610104995613828 29.429956296959897 33.271366936851194 17.68857177057508 62-63 18.277120690136925 29.40243257535695 32.401394596507714 19.91905213799841 64-65 17.493520049728488 33.13639941744264 30.18908277571611 19.180997757112767 66-67 19.059298185337962 29.160259156616448 28.25016850975287 23.530274148292722 68-69 17.188548878379265 29.83624992669709 30.15995556981369 22.815245625109952 70-71 21.281764174654064 29.611389258563985 28.442652526335472 20.66419404044648 72-73 19.84196303341012 26.47317538605464 32.975858088916574 20.709003491618667 74-75 17.059879086922056 33.56390020911077 28.93100310389101 20.445217600076166 76-77 18.654262759721785 28.437240485655273 29.400896549673593 23.507600204949348 78-79 18.744183222706766 29.095442406229584 30.71920721421815 21.4411671568455 80-81 20.819384120025845 28.552120815106647 29.33113802468446 21.297357040183048 82-83 18.854395845104868 27.958924324805395 32.142968020746885 21.04371180934286 84-85 17.991632809487047 29.491655921367176 30.702649449350833 21.814061819794947 86-87 17.98491246474056 30.296650625182792 29.850074222220535 21.868362687856113 88-89 17.795820485519297 29.727222504524136 28.00040014565856 24.476556864298008 90-91 18.774440116457576 26.829999421935106 29.667951909154507 24.72760855245281 92-93 20.457911886474445 29.861618480121365 29.620220066493225 20.06024956691096 94-95 19.16892438689701 28.47131815676202 29.829986250156093 22.529771206184876 96-97 15.599001290815753 28.929761271525955 32.28953873516614 23.181698702492152 98-99 18.30391915395341 30.19884495791913 27.763301231966697 23.733934656160766 100-101 17.37453655346176 27.829317831532443 29.71635761031994 25.079788004685867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2559.0 1 1564.0 2 648.0 3 722.5 4 611.0 5 640.0 6 1231.0 7 2432.5 8 3539.0 9 3835.5 10 3409.0 11 3017.5 12 3185.0 13 3415.5 14 3564.0 15 3806.0 16 4120.0 17 4512.5 18 4863.0 19 5293.5 20 6025.0 21 6802.0 22 7499.5 23 8261.5 24 9201.5 25 10895.5 26 14262.0 27 17926.0 28 19147.0 29 20378.5 30 23570.5 31 26606.0 32 30634.0 33 36313.5 34 42689.0 35 46605.5 36 50567.5 37 55645.5 38 53199.0 39 47025.5 40 43058.0 41 39573.5 42 35941.0 43 34039.5 44 33755.5 45 34032.5 46 40712.5 47 86116.5 48 182399.5 49 172704.5 50 74005.5 51 29994.5 52 13895.5 53 8368.5 54 6331.0 55 5080.5 56 4145.0 57 3280.0 58 2504.5 59 1910.5 60 1457.5 61 1229.0 62 1037.5 63 833.5 64 690.5 65 583.5 66 518.5 67 470.0 68 371.5 69 316.5 70 282.5 71 230.0 72 185.5 73 137.0 74 110.5 75 79.5 76 51.5 77 33.0 78 25.5 79 18.5 80 9.0 81 3.5 82 2.5 83 1.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26319947070344973 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002552655417045253 16-17 4.484394651565985E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.4495343473584495E-5 26-27 0.001241832365049042 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003518525034305619 36-37 0.02145610364056956 38-39 0.008071910372818772 40-41 0.01838601807142054 42-43 0.005208796864511259 44-45 6.8990686947169E-4 46-47 1.3798137389433798E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.4495343473584495E-5 58-59 5.519254955773519E-4 60-61 0.0020352252649414853 62-63 0.0013453183954697954 64-65 0.0 66-67 0.0 68-69 4.13944121683014E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22490963944777095 78-79 0.0 80-81 0.0011383463346282886 82-83 0.0 84-85 0.0 86-87 0.10034695416465732 88-89 0.34474646267500353 90-91 0.3447119673315299 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.44953434735845E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1449471.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.75821463173902 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31518122349247 17.418378112725765 2 12.651592621951375 6.011585058912932 3 4.792175256646136 3.4156058490092374 4 2.4714068599398047 2.348648584832082 5 1.4514242396874681 1.7241624304101741 6 0.9030233696076253 1.2872533819568492 7 0.6463300101210363 1.0748952972372745 8 0.46291448973947763 0.8798417442697968 9 0.3571406351625492 0.7636521477516698 >10 2.2506908520918105 10.581119713348713 >50 0.3608363409562081 6.070126190113805 >100 0.2942513015617028 13.973757888248425 >500 0.022679448143945156 3.6634450986099236 >1k 0.012502772694738998 5.603415846540654 >5k 0.0040706701796824635 6.9673293332459325 >10k+ 0.003779908023990859 18.21678332278678 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 61555 4.246721735032988 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 33214 2.2914566762632713 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24661 1.701379330804135 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19028 1.3127547912307318 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16414 1.132413135550832 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 15995 1.1035060377199681 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15554 1.0730811447762667 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 14378 0.9919480969263959 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14327 0.9884295718920904 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13227 0.9125398162502044 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12701 0.8762507149159935 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12496 0.8621076240918238 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10156 0.7006694166354483 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9779 0.6746599276563656 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9455 0.6523069450854829 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8318 0.5738645340265518 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8138 0.5614462103760613 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8047 0.555168057863869 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8039 0.5546161323682917 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7840 0.540886985665805 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7005 0.4832797620649189 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6951 0.4795542649697717 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5884 0.4059412019971424 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5504 0.3797247409572182 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5429 0.3745504394361805 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5403 0.3727566815755541 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5067 0.34957581076130534 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4241 0.29258950334294376 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3992 0.27541082229309866 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3552 0.2450549200363443 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3446 0.2377419072199444 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3235 0.22318487227409173 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2897 0.1998660200859486 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2862 0.19745134604279768 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2708 0.18682678025293367 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2670 0.18420513414894124 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2501 0.17254570805486968 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2397 0.1653706766123641 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2353 0.16233508638668867 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2334 0.16102426333469244 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2204 0.15205547403156047 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2173 0.14991676273619825 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1986 0.13701550427707765 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1921 0.13253110962551165 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1783 0.12301039482680233 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1725 0.11900893498386653 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1671 0.1152834378887194 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1643 0.11335169865419868 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 0.10803941575926665 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1528 0.10541776965527422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.44953434735845E-5 0.0 14-15 0.0 0.0 0.0 1.37981373894338E-4 0.0 16-17 0.0 0.0 0.0 3.104580912622605E-4 0.0 18-19 0.0 0.0 0.0 5.51925495577352E-4 0.0 20-21 0.0 0.0 0.0 8.27888243366028E-4 6.8990686947169E-5 22-23 0.0 0.0 0.0 0.001724767173679225 6.8990686947169E-5 24-25 0.0 0.0 0.0 0.0040704505298829715 6.8990686947169E-5 26-27 0.0 0.0 0.0 0.007209526785979161 6.8990686947169E-5 28-29 0.0 0.0 0.0 0.018592990132262045 6.8990686947169E-5 30-31 0.0 0.0 0.0 0.04925935048027867 6.8990686947169E-5 32-33 0.0 0.0 0.0 0.10890179934610628 6.8990686947169E-5 34-35 0.0 0.0 0.0 0.19814125291226936 6.8990686947169E-5 36-37 0.0 0.0 0.0 0.3131142327097265 6.8990686947169E-5 38-39 0.0 0.0 0.0 0.48724672656438106 6.8990686947169E-5 40-41 0.0 0.0 0.0 0.6737630487260524 6.8990686947169E-5 42-43 0.0 0.0 0.0 0.8716973295774804 6.8990686947169E-5 44-45 0.0 0.0 0.0 1.101125859020291 6.8990686947169E-5 46-47 0.0 0.0 0.0 1.393922334424076 6.8990686947169E-5 48-49 0.0 0.0 0.0 1.6651937155003447 6.8990686947169E-5 50-51 0.0 0.0 0.0 1.9708914493632506 6.8990686947169E-5 52-53 0.0 0.0 0.0 2.2661025988101864 6.8990686947169E-5 54-55 0.0 0.0 0.0 2.592911482878926 6.8990686947169E-5 56-57 0.0 0.0 0.0 3.0428687431483623 6.8990686947169E-5 58-59 0.0 0.0 0.0 3.478613921906682 6.8990686947169E-5 60-61 0.0 0.0 0.0 3.981418048377649 6.8990686947169E-5 62-63 0.0 0.0 0.0 4.4536593005310205 6.8990686947169E-5 64-65 0.0 0.0 0.0 4.923692850702084 6.8990686947169E-5 66-67 0.0 0.0 0.0 5.4682363427760885 6.8990686947169E-5 68-69 0.0 0.0 0.0 6.1145066027536945 6.8990686947169E-5 70-71 0.0 0.0 0.0 6.799032198643506 6.8990686947169E-5 72-73 0.0 0.0 0.0 7.435160827639876 6.8990686947169E-5 74-75 0.0 0.0 0.0 8.172119345609536 6.8990686947169E-5 76-77 0.0 0.0 0.0 8.827289404203327 6.8990686947169E-5 78-79 0.0 0.0 0.0 9.779843818882888 6.8990686947169E-5 80-81 0.0 0.0 0.0 10.77493099206538 6.8990686947169E-5 82-83 0.0 0.0 0.0 11.622309104493983 6.8990686947169E-5 84-85 0.0 0.0 0.0 12.57017215246114 6.8990686947169E-5 86-87 0.0 0.0 0.0 13.535627825599821 6.8990686947169E-5 88-89 0.0 0.0 0.0 14.560553470886965 6.8990686947169E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 275 0.0 41.409027 94-95 CTTTGCG 185 0.0 35.906475 2 CATATAG 475 0.0 29.967056 3 CGGAGAT 65 0.0058001527 29.276436 1 TGGGCGA 65 0.005876031 29.19867 6 ATGCCGC 75 3.3762262E-8 28.468706 94-95 GCTTATG 470 0.0 27.329863 1 CTCGAGT 70 0.008434536 27.114923 9 ATAACGC 70 0.008437375 27.113052 3 CATGGGT 1190 0.0 26.714333 4 ATGGGCG 125 7.4559903E-6 26.570793 5 GCTTTGC 275 0.0 25.949568 1 TAGGGTC 265 1.8189894E-12 25.066786 4 TAGGACG 95 0.0012345909 24.972548 4 GAGTACT 11235 0.0 24.581667 12-13 GACATAT 535 0.0 24.009413 1 ATGGGTA 660 0.0 23.72392 5 CATTCCG 285 3.6379788E-12 23.30932 9 ATGGGGA 1825 0.0 23.138948 5 AGTACTT 11515 0.0 23.056723 12-13 >>END_MODULE