FastQCFastQC Report
Fri 27 May 2016
SRR939331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3463864
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92750.26776455426656476No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75980.21935041329567215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.2059549682089135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47570.13733218163299712No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39210.11319728488185449No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144600.028.586051
TACATGG144050.027.8774642
ACATGGG145250.026.7005523
CATGGGG69500.023.0576484
GTATCAA211400.022.1123811
GAGTACT94700.021.66351312-13
CATGGGA85350.020.4977364
GTACTTT101100.020.22622514-15
ATCAACG236500.019.4657523
TCAACGC237800.019.3992144
CAACGCA238450.019.3860975
CCGTAAG4700.019.1849211
AGTACTT101250.019.16240112-13
AACGCAG242950.019.053926
GTACCGT2251.7100847E-618.9525096
TATCAAC247500.018.7730142
AGTACAT106900.018.6718562
GGTATCA164650.018.418041
AGAGTAC176700.018.31310-11
ATGGGGA45650.018.2791085
Produced by FastQC (version 0.11.5)