FastQCFastQC Report
Fri 27 May 2016
SRR939331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3463864
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94830.27376940896062896No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82010.236758718009714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78330.2261347443202158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54150.15632830850171947No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36140.1043343503093655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG147000.025.5480041
TACATGG147400.025.1109642
ACATGGG147300.024.1615493
GAGTACT100500.022.02936612-13
GTATCAA219900.021.6673681
GTACTTT107250.020.48802614-15
CATGGGG74200.020.1452624
AGTACTT108500.019.63961612-13
CATGGGA81700.019.051014
CTAGCGT1250.006067929318.9813124
GGTATCA173550.018.9518131
ATCAACG251550.018.8832193
TCAACGC253650.018.7081744
CAACGCA253550.018.6968365
TATCAAC258200.018.4703922
AACGCAG258800.018.2808826
AGAGTAC195250.018.07187510-11
ACTTTTT129950.017.01733616-17
AGTACAT114600.016.9771792
CTAGACG2253.57092E-516.8722783
Produced by FastQC (version 0.11.5)