##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939331_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3463864 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.329750821625794 34.0 31.0 34.0 31.0 34.0 2 32.53292248194502 34.0 31.0 34.0 31.0 34.0 3 32.62563743842137 34.0 31.0 34.0 31.0 34.0 4 36.14121426245372 37.0 35.0 37.0 35.0 37.0 5 36.0853402443052 37.0 35.0 37.0 35.0 37.0 6 36.117529441109696 37.0 36.0 37.0 35.0 37.0 7 36.05838739627191 37.0 35.0 37.0 35.0 37.0 8 36.031658575509894 37.0 35.0 37.0 35.0 37.0 9 37.739684352503446 39.0 38.0 39.0 35.0 39.0 10-11 37.66375787271094 39.0 37.0 39.0 35.0 39.0 12-13 37.59282061882337 39.0 37.0 39.0 35.0 39.0 14-15 38.90333988285914 40.0 38.0 41.0 35.0 41.0 16-17 38.90943654254324 40.0 38.0 41.0 35.0 41.0 18-19 39.004995721541036 40.0 38.5 41.0 35.0 41.0 20-21 38.98514101592903 40.0 38.5 41.0 35.0 41.0 22-23 38.906265661700346 40.0 38.0 41.0 35.0 41.0 24-25 38.8435194049189 40.0 38.0 41.0 35.0 41.0 26-27 38.69295517953361 40.0 38.0 41.0 34.0 41.0 28-29 38.5357421653968 40.0 38.0 41.0 34.0 41.0 30-31 38.357524285018116 40.0 38.0 41.0 34.0 41.0 32-33 38.31669156179342 40.0 38.0 41.0 34.0 41.0 34-35 38.22900365025879 40.0 38.0 41.0 34.0 41.0 36-37 38.0834481376867 40.0 38.0 41.0 33.0 41.0 38-39 37.96229456468268 40.0 38.0 41.0 33.0 41.0 40-41 37.79030138019276 40.0 37.0 41.0 33.0 41.0 42-43 37.618813556190425 40.0 37.0 41.0 33.0 41.0 44-45 37.3896179237984 40.0 37.0 41.0 32.0 41.0 46-47 37.10728077083858 40.0 37.0 41.0 31.0 41.0 48-49 36.814484633345884 40.0 36.0 41.0 31.0 41.0 50-51 36.576847849684626 39.0 36.0 41.0 30.5 41.0 52-53 36.325216578941905 39.0 35.0 41.0 30.0 41.0 54-55 36.085987354006974 39.0 35.0 40.0 29.5 41.0 56-57 35.7779993671807 38.5 35.0 40.0 28.5 41.0 58-59 35.462405134843635 38.0 35.0 40.0 28.0 41.0 60-61 35.11644236032362 38.0 34.0 40.0 28.0 41.0 62-63 34.772759698417715 37.0 34.0 40.0 27.0 41.0 64-65 34.46334065078767 37.0 34.0 40.0 26.5 41.0 66-67 34.04913934265318 36.5 33.5 39.0 26.0 40.5 68-69 33.58567065566085 36.0 33.0 39.0 26.0 40.0 70-71 33.10709037652749 35.0 32.5 38.5 25.0 40.0 72-73 32.61803884332642 35.0 32.0 37.5 24.0 39.5 74-75 32.1012525318546 35.0 31.5 37.0 22.5 39.0 76-77 30.146713901007658 33.5 29.5 35.0 20.0 37.5 78-79 31.111795815309144 34.0 31.0 36.0 20.0 38.0 80-81 31.120918142282722 34.0 31.0 36.0 20.0 37.0 82-83 30.777152191887442 34.0 31.0 35.0 19.5 37.0 84-85 30.33250266176732 34.0 30.5 35.0 17.5 36.5 86-87 29.740883013882762 34.0 30.0 35.0 10.0 36.0 88-89 29.154482393073167 34.0 29.0 35.0 6.0 35.5 90-91 28.668155707036995 34.0 29.0 35.0 2.0 35.0 92-93 28.308704816355377 33.0 29.0 35.0 2.0 35.0 94-95 27.791167753699337 33.0 27.5 35.0 2.0 35.0 96-97 27.055318713436783 33.0 26.5 34.5 2.0 35.0 98-99 26.050800926364314 32.5 24.5 34.0 2.0 35.0 100-101 21.695048795218288 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24731088986850835 1101 2 0.24446126296361825 1101 3 0.28089653721497143 1101 4 0.128500194918864 1101 5 0.20146987463882482 1101 6 0.17249387379863634 1101 7 0.2692041710781794 1101 8 0.2241075016331706 1101 9 0.30395249099076693 1101 10-11 0.33479327430649874 1101 12-13 0.29142989302820865 1101 14-15 0.3308151181671519 1101 16-17 0.37813919635544124 1101 18-19 0.3745367484122042 1101 20-21 0.31072766274317587 1101 22-23 0.30938769477845085 1101 24-25 0.31453611659558334 1101 26-27 0.34403743474759807 1101 28-29 0.31733118838151597 1101 30-31 0.3800508834231593 1101 32-33 0.44796363774882764 1101 34-35 0.4502080591777897 1101 36-37 0.46575184795892 1101 38-39 0.4877982691731404 1101 40-41 0.4594301422256848 1101 42-43 0.5136570994636571 1101 44-45 0.6133969963306498 1101 46-47 0.6251094674585502 1101 48-49 0.6329516661025778 1101 50-51 0.6274076290322839 1101 52-53 0.6111357837928821 1101 54-55 0.5756333495329571 1101 56-57 0.4744838805490659 1101 58-59 0.5933446951626706 1101 60-61 0.5597935900287538 1101 62-63 0.5325622109554402 1101 64-65 0.5894833979272036 1101 66-67 0.5082571206677287 1101 68-69 0.5096653191868299 1101 70-71 0.4998847949435614 1101 72-73 0.5269069795072028 1101 74-75 0.5949158211553041 1101 76-77 0.5717392310261005 1101 78-79 0.6319047080476885 1101 80-81 0.7921073089460648 1101 82-83 0.8177776586706145 1101 84-85 0.7999253295619724 1101 86-87 0.6454996172287544 1101 88-89 0.800670444449441 1101 90-91 0.8590319989298472 1101 92-93 0.8590934133314292 1101 94-95 0.6826183175823388 1101 96-97 0.640023729691432 1101 98-99 0.40640012721140195 1101 100-101 0.12525252271439413 1102 1 0.19080545336941412 1102 2 0.18948990425097634 1102 3 0.18090482070454783 1102 4 0.10286707924136351 1102 5 0.20794670657539172 1102 6 0.20834444943995578 1102 7 0.12035618820990379 1102 8 0.16508212967184477 1102 9 0.265983912755857 1102 10-11 0.24035496426895264 1102 12-13 0.2338015948323786 1102 14-15 0.28137890552547873 1102 16-17 0.3047235055491342 1102 18-19 0.26951403614472014 1102 20-21 0.3759908492016635 1102 22-23 0.33208180387078556 1102 24-25 0.3057209330816022 1102 26-27 0.2762336041029272 1102 28-29 0.3313211045591231 1102 30-31 0.3398669817934774 1102 32-33 0.43957160589452826 1102 34-35 0.43760491341040364 1102 36-37 0.39983189911252737 1102 38-39 0.43466162179108636 1102 40-41 0.5155040619647906 1102 42-43 0.5458409047926693 1102 44-45 0.6351442168730514 1102 46-47 0.6215081897608883 1102 48-49 0.6859401900010553 1102 50-51 0.5399878421832014 1102 52-53 0.5561494291077338 1102 54-55 0.4475648879197891 1102 56-57 0.5137057956428919 1102 58-59 0.5771233374701623 1102 60-61 0.5589641189234484 1102 62-63 0.4388648243759903 1102 64-65 0.5159004051146567 1102 66-67 0.5252188917512512 1102 68-69 0.4426952442110519 1102 70-71 0.333541918867013 1102 72-73 0.3544360553717283 1102 74-75 0.4518169064964681 1102 76-77 0.5225426479043449 1102 78-79 0.42163740432762964 1102 80-81 0.5790499382317513 1102 82-83 0.516748608513474 1102 84-85 0.42220646793379046 1102 86-87 0.373231924225486 1102 88-89 0.5768300513794955 1102 90-91 0.5847665771533315 1102 92-93 0.4939857565306838 1102 94-95 0.41670810081606646 1102 96-97 0.2085772569481925 1102 98-99 0.022598409468653102 1102 100-101 0.10029565398770757 1103 1 0.10371331399245065 1103 2 0.15328251640512036 1103 3 0.2388392900944325 1103 4 0.18592977639907815 1103 5 0.188070399214034 1103 6 0.18681542862652378 1103 7 0.2307598949756695 1103 8 0.2377932941164289 1103 9 0.20043604506925305 1103 10-11 0.2862266923538428 1103 12-13 0.21794680324764926 1103 14-15 0.24854396817448077 1103 16-17 0.24621321522059958 1103 18-19 0.24649213508278223 1103 20-21 0.30801498170770714 1103 22-23 0.29962583074443216 1103 24-25 0.35776836330884265 1103 26-27 0.30743609561461227 1103 28-29 0.32472289292511647 1103 30-31 0.3419696964495671 1103 32-33 0.2977341360952721 1103 34-35 0.3783862599825696 1103 36-37 0.44475295575749385 1103 38-39 0.4298742511225413 1103 40-41 0.5036209198025361 1103 42-43 0.5190258497691218 1103 44-45 0.5362899390020743 1103 46-47 0.5795492146198171 1103 48-49 0.4624270501665748 1103 50-51 0.4637206147123507 1103 52-53 0.36784875806915096 1103 54-55 0.4127670021729344 1103 56-57 0.5651189097916927 1103 58-59 0.5791768881151143 1103 60-61 0.5854468653655758 1103 62-63 0.5742991332303333 1103 64-65 0.7139523062568713 1103 66-67 0.5792523473591586 1103 68-69 0.5607617139541077 1103 70-71 0.5418113055409535 1103 72-73 0.661217578532991 1103 74-75 0.6014831932290079 1103 76-77 0.625298441230882 1103 78-79 0.749003497614833 1103 80-81 0.851668995406488 1103 82-83 0.9053452669660693 1103 84-85 0.8232129254761524 1103 86-87 0.768166927396063 1103 88-89 0.906843977838161 1103 90-91 1.0034752894174446 1103 92-93 0.9004350714452372 1103 94-95 0.6762038787311937 1103 96-97 0.47414127852416854 1103 98-99 0.29798961452873485 1103 100-101 0.35188896928754687 1104 1 0.1786076976413895 1104 2 0.14595447241207893 1104 3 0.12697210314758678 1104 4 0.06301830532261476 1104 5 0.09567280970565406 1104 6 0.07451240213786292 1104 7 0.07343499783852536 1104 8 0.13489287637131042 1104 9 0.20091240038075142 1104 10-11 0.14009073637835456 1104 12-13 0.12646406375224473 1104 14-15 0.17450415258023355 1104 16-17 0.1781375766055291 1104 18-19 0.1516898559723998 1104 20-21 0.22711787209484413 1104 22-23 0.17059972364119602 1104 24-25 0.1618464918736393 1104 26-27 0.20384867989135103 1104 28-29 0.20544466366092706 1104 30-31 0.22956311873402768 1104 32-33 0.18344927876584904 1104 34-35 0.15200410186879765 1104 36-37 0.2651123267010007 1104 38-39 0.2701081805228256 1104 40-41 0.3231875780775866 1104 42-43 0.2345669909764041 1104 44-45 0.21576939003306705 1104 46-47 0.22437834034457893 1104 48-49 0.21974496521719544 1104 50-51 0.21642848421224414 1104 52-53 0.24151675297124342 1104 54-55 0.2323937457277836 1104 56-57 0.227285048048806 1104 58-59 0.13664840906923814 1104 60-61 0.26514138432931844 1104 62-63 0.4098734540994968 1104 64-65 0.40724163605523955 1104 66-67 0.25251980650902794 1104 68-69 0.28036682287466164 1104 70-71 0.23282722863179828 1104 72-73 0.21094606387257642 1104 74-75 0.30951888644202796 1104 76-77 0.17891988233354184 1104 78-79 0.41120228450188634 1104 80-81 0.5587722358813458 1104 82-83 0.5393707446981324 1104 84-85 0.5847580513897235 1104 86-87 0.44929651098835777 1104 88-89 0.5901414501261648 1104 90-91 0.5744936316537554 1104 92-93 0.5753670206864534 1104 94-95 0.40833986617523976 1104 96-97 0.3231239590160264 1104 98-99 0.5110548685193415 1104 100-101 0.40562153373909027 1105 1 0.06887578780737158 1105 2 0.09836409337857077 1105 3 0.1039685346038226 1105 4 0.06414262273199967 1105 5 0.0934067147641855 1105 6 0.07052510165792825 1105 7 0.09969292335077995 1105 8 0.08612551332879548 1105 9 0.07401175153206196 1105 10-11 0.07756866077239977 1105 12-13 0.15410568013599146 1105 14-15 0.17526819991682174 1105 16-17 0.177488355813594 1105 18-19 0.17171604424823528 1105 20-21 0.173642811542571 1105 22-23 0.14562339975840644 1105 24-25 0.1285076279112758 1105 26-27 0.15763197362613823 1105 28-29 0.15927699839609488 1105 30-31 0.1776723019380313 1105 32-33 0.14227554253048424 1105 34-35 0.2730625268741349 1105 36-37 0.19432097478564714 1105 38-39 0.2917032037581464 1105 40-41 0.3900192031494143 1105 42-43 0.2867955609656434 1105 44-45 0.21865202766796443 1105 46-47 0.1792910559508627 1105 48-49 0.2916582969845294 1105 50-51 0.264506458639552 1105 52-53 0.3188348037040569 1105 54-55 0.37927526286169666 1105 56-57 0.32045231049048084 1105 58-59 0.3366356526845138 1105 60-61 0.33818857455668905 1105 62-63 0.3310283105038181 1105 64-65 0.2930947958508554 1105 66-67 0.364657492266133 1105 68-69 0.4621801777885821 1105 70-71 0.36432279977149307 1105 72-73 0.3678814631359657 1105 74-75 0.30781337593189306 1105 76-77 0.5014575804558419 1105 78-79 0.4836636065136801 1105 80-81 0.6295079634713723 1105 82-83 0.6111831798547662 1105 84-85 0.6227786612040696 1105 86-87 0.65347165567362 1105 88-89 0.8066649088028193 1105 90-91 0.9109006303578866 1105 92-93 0.919102071052702 1105 94-95 0.9180480760394261 1105 96-97 0.7680787277571746 1105 98-99 0.9161778497999897 1105 100-101 0.4923022174813134 1106 1 0.10150205408332624 1106 2 0.12332103005520167 1106 3 0.11933506444710673 1106 4 0.12775432782172658 1106 5 0.07821666874221478 1106 6 0.12253580979562884 1106 7 0.10033167858060921 1106 8 0.015512829407505535 1106 9 0.09064743972000855 1106 10-11 0.18085677042913773 1106 12-13 0.15815305151659942 1106 14-15 0.16301128958567546 1106 16-17 0.1820399861373616 1106 18-19 0.12330768501578149 1106 20-21 0.19058512221328527 1106 22-23 0.2707827935183218 1106 24-25 0.308487172492228 1106 26-27 0.2405432376697192 1106 28-29 0.24385414624687485 1106 30-31 0.21198229104942357 1106 32-33 0.14851797683066081 1106 34-35 0.32155644989341425 1106 36-37 0.33156474415324766 1106 38-39 0.4080893361969089 1106 40-41 0.4284467849561082 1106 42-43 0.37790719479004053 1106 44-45 0.4982661309040992 1106 46-47 0.3527519109495998 1106 48-49 0.26046478156661124 1106 50-51 0.3640065664000005 1106 52-53 0.29324446804526616 1106 54-55 0.28552168257326827 1106 56-57 0.33679472142288347 1106 58-59 0.356274834053778 1106 60-61 0.43508718006214764 1106 62-63 0.38858445986650736 1106 64-65 0.3351116082952146 1106 66-67 0.45391118903025074 1106 68-69 0.5571014200676387 1106 70-71 0.446341431524381 1106 72-73 0.4535860557279747 1106 74-75 0.4933389972248463 1106 76-77 0.5062551442528367 1106 78-79 0.5356588360034742 1106 80-81 0.5033835826853768 1106 82-83 0.609943980183477 1106 84-85 0.5432459184621017 1106 86-87 0.5902027050424259 1106 88-89 0.812067274666493 1106 90-91 0.8519286613268058 1106 92-93 0.8765800010427363 1106 94-95 1.0439266141360548 1106 96-97 0.9140555865884572 1106 98-99 1.0023369971725167 1106 100-101 0.8940050866325429 1107 1 0.07312143851927289 1107 2 0.09069807640644001 1107 3 0.11875454836104637 1107 4 0.016224671779426103 1107 5 0.065049504543218 1107 6 0.05666701172791733 1107 7 2.9666820650220416E-4 1107 8 0.06711212345894069 1107 9 0.07219129982477313 1107 10-11 0.11431147248982398 1107 12-13 0.08933225012588508 1107 14-15 0.14720143162801236 1107 16-17 0.15975081685032677 1107 18-19 0.12475786460414184 1107 20-21 0.17333994997984092 1107 22-23 0.19993364585604212 1107 24-25 0.2036672401242754 1107 26-27 0.2514171283884892 1107 28-29 0.245866174847734 1107 30-31 0.27070997236309324 1107 32-33 0.1561821133755643 1107 34-35 0.196383683351101 1107 36-37 0.21822809830428724 1107 38-39 0.17289604185398133 1107 40-41 0.1244526377884867 1107 42-43 0.13216849896321037 1107 44-45 0.18341966480752347 1107 46-47 0.2163150392333293 1107 48-49 0.1512889708816587 1107 50-51 0.28994629763856494 1107 52-53 0.28138553678606115 1107 54-55 0.36461078406080816 1107 56-57 0.4085045194237935 1107 58-59 0.45385407770325514 1107 60-61 0.44721616449987067 1107 62-63 0.364851134005697 1107 64-65 0.4162235456367256 1107 66-67 0.5006323538693067 1107 68-69 0.5581312274114794 1107 70-71 0.500897085973186 1107 72-73 0.5644616380167875 1107 74-75 0.395511314903068 1107 76-77 0.4083873085005294 1107 78-79 0.5225424616224537 1107 80-81 0.5835380315744167 1107 82-83 0.689501680748581 1107 84-85 0.5435290428637494 1107 86-87 0.6308330643506999 1107 88-89 0.8125331398934712 1107 90-91 0.8747933488792938 1107 92-93 0.7985272852270136 1107 94-95 0.8596698686587416 1107 96-97 0.7991565024113179 1107 98-99 0.8984780132866881 1107 100-101 0.6147282379557844 1108 1 0.029013582696521212 1108 2 0.038424206542927664 1108 3 0.01189927908727384 1108 4 0.0018210861425700386 1108 5 0.03572292564379609 1108 6 0.01749607366016903 1108 7 -0.015874020010244294 1108 8 0.05312120660595099 1108 9 0.011938701534518259 1108 10-11 0.012068101197691306 1108 12-13 -0.02758500239333017 1108 14-15 -0.016590060120385886 1108 16-17 -0.009488338011230724 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-1.113616473718622 2316 92-93 -0.8082117123070311 2316 94-95 -0.7581081231440976 2316 96-97 -0.6747370017359664 2316 98-99 -0.6634609426496674 2316 100-101 -0.008445277650071858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 24.0 5 58.0 6 137.0 7 364.0 8 885.0 9 1624.0 10 2731.0 11 4445.0 12 6301.0 13 8560.0 14 11055.0 15 14466.0 16 18727.0 17 25848.0 18 25186.0 19 18370.0 20 17309.0 21 18538.0 22 19569.0 23 22202.0 24 25749.0 25 30704.0 26 37020.0 27 44912.0 28 54938.0 29 67149.0 30 82004.0 31 101010.0 32 125038.0 33 161714.0 34 220074.0 35 319118.0 36 511389.0 37 812138.0 38 613029.0 39 41461.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81517599012087 19.332203159179386 14.434186658250681 23.418434192449066 2 15.530517364423083 20.659904661383933 39.61486363205945 24.194714342133526 3 19.48078792931824 24.267407727324166 29.77879616520741 26.47300817815018 4 13.032151377767716 16.566239321174272 36.726095481808755 33.675513819249254 5 13.736133982165583 37.65318153368608 34.09553608340281 14.515148400745526 6 32.37413478127317 36.08828175702048 17.086583076009912 14.451000385696435 7 28.97478076506468 32.64573897820468 20.459463766475817 17.920016490254813 8 26.218783416438985 35.621259957088384 19.484194529577373 18.67576209689526 9 26.300743909114217 17.05895497051847 20.317223770910175 36.32307734945714 10-11 25.158276999327917 26.61664834416132 27.05018153137652 21.174893125134243 12-13 25.852083107188967 23.89123822413351 27.780825113226154 22.47585355545137 14-15 22.581373891182754 25.43828702711972 26.076887233943047 25.90345184775448 16-17 22.167289436054723 28.762086696543044 26.735557673314702 22.335066194087524 18-19 22.059715724777103 27.36689962921439 28.898499550285138 21.67488509572337 20-21 22.784936933129284 26.50539296733156 28.72571750095197 21.983952598587184 22-23 22.468462965058674 26.441453821512624 28.749281149606336 22.34080206382237 24-25 22.375936237681387 26.602920899896766 28.871514586023007 22.149628276398843 26-27 22.212273468169343 26.698191209696 28.933557399226643 22.155977922908015 28-29 22.000863775252146 27.056864819173036 28.999406443209086 21.942864962365725 30-31 22.244464563273848 26.618106253594252 28.72391929937203 22.413509883759872 32-33 21.979139481226746 26.866614855548605 28.722244868736187 22.432000794488467 34-35 22.559182680107362 26.783838254361026 28.512758642292347 22.144220423239272 36-37 22.609850137854465 26.505128382429177 28.618627379792187 22.26639409992417 38-39 22.213559545166632 26.904396764915955 28.668469709628862 22.213573980288555 40-41 22.3402025544495 26.788331177679748 28.630202438968055 22.241263828902696 42-43 22.229818133107305 26.800192386972654 28.324045022708823 22.645944457211222 44-45 22.32924444511166 26.750030349453937 28.343719510707622 22.577005694726786 46-47 22.58626214685351 27.238037911462936 27.898500516764535 22.27719942491902 48-49 22.566096270362827 27.119253754999136 27.82107418654797 22.49357578809007 50-51 22.281388645743597 27.31214331740507 27.989421640110585 22.41704639674075 52-53 22.650392531685384 27.32581176407075 27.55748968528034 22.46630601896352 54-55 22.273420665476472 27.107805040844557 27.703873477711593 22.91490081596737 56-57 22.49032790013595 27.300120762312456 27.668484789520498 22.541066548031093 58-59 22.650804499974377 26.959500374949748 27.677108722435417 22.712586402640454 60-61 22.429546750073147 27.21433830868896 27.947426547910204 22.408688393327694 62-63 22.257202799100533 27.24340736416686 28.00842825002519 22.49096158670742 64-65 22.09644261334816 27.184418090438335 27.87656440222953 22.842574893983972 66-67 22.127645323928764 27.23497104953797 27.605916887808007 23.031466738725257 68-69 22.150194857854377 27.105465171702697 27.814092990746886 22.93024697969604 70-71 22.325212537212778 27.11277059376465 27.664712009478432 22.89730485954414 72-73 22.320665591951645 26.92656235926122 27.775123388216166 22.97764866057097 74-75 22.13163453635435 27.563691173698267 27.31428754543699 22.990386744510392 76-77 22.23283989380978 27.081589449517384 27.497878395291135 23.187692261381702 78-79 22.24357125597275 27.211645861823975 27.572659610370753 22.972123271832526 80-81 22.294796963620374 27.38607946852386 27.378429048270398 22.940694519585364 82-83 22.26149756456951 27.229720912830295 27.494555213484134 23.01422630911606 84-85 22.174788040177095 27.20132776575524 27.413302023405077 23.21058217066259 86-87 22.151118599590188 27.193949360284154 27.373436836188237 23.281495203937425 88-89 22.14478024422042 27.09046251426078 27.31559225581508 23.449164985703725 90-91 21.99821003189837 27.161615186386978 27.400928971153377 23.439245810561275 92-93 22.164625978387143 27.04478293605061 27.407600298395085 23.382990787167163 94-95 21.906518269770405 26.905747454288043 27.537585193875973 23.650149082065578 96-97 21.897770813172805 26.78267680255345 27.62202846301125 23.697523921262498 98-99 21.86591467355147 27.004688264816597 27.13926350692717 23.99013355470477 100-101 21.800820964281545 26.37787654703064 27.897109389874604 23.924193098813216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 618.0 1 420.5 2 311.0 3 415.5 4 372.5 5 377.0 6 697.0 7 1466.5 8 2359.0 9 2791.5 10 2602.0 11 2400.0 12 2549.0 13 2765.5 14 3171.0 15 3493.5 16 3598.5 17 3801.5 18 4085.5 19 4443.0 20 5036.5 21 6068.0 22 7418.0 23 9309.0 24 11456.0 25 14144.0 26 18491.0 27 23436.5 28 27584.0 29 32785.5 30 39536.0 31 46452.5 32 53714.5 33 59636.0 34 65120.5 35 71115.5 36 79818.5 37 89989.5 38 95032.5 39 96925.5 40 99325.5 41 104118.0 42 109047.0 43 114501.0 44 121772.5 45 128176.5 46 129747.5 47 134915.5 48 150742.5 49 150733.5 50 135777.5 51 127292.0 52 122869.5 53 120942.0 54 117788.0 55 109513.0 56 97177.0 57 86300.0 58 77927.0 59 67786.5 60 57226.0 61 47294.0 62 38380.5 63 29975.5 64 22654.0 65 16950.5 66 12445.0 67 9991.0 68 7776.0 69 5332.0 70 3662.0 71 2609.0 72 2023.5 73 1683.5 74 1323.5 75 1012.5 76 678.0 77 364.0 78 201.0 79 137.0 80 84.5 81 43.0 82 26.5 83 20.5 84 13.5 85 3.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2921304069674791 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010970407614155752 16-17 0.0010104322802511876 18-19 7.217373430365627E-5 20-21 2.8869493721462508E-5 22-23 0.0 24-25 0.0 26-27 5.7738987442925016E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001587822154680438 36-37 0.00714519969606197 38-39 0.002598254434931626 40-41 0.0030024273470321008 42-43 3.4643392465755007E-4 44-45 0.001313561964326544 46-47 1.1547797488585003E-4 48-49 4.3304240582193754E-4 50-51 0.0 52-53 1.0104322802511877E-4 54-55 0.0 56-57 0.0010104322802511876 58-59 0.0019198213324772566 60-61 7.073025961758314E-4 62-63 0.0010681712676941127 64-65 2.8869493721462508E-5 66-67 4.619118995434001E-4 68-69 0.0016455611421233628 70-71 0.0 72-73 0.0 74-75 2.8869493721462508E-5 76-77 0.2578623179201031 78-79 2.0208645605023753E-4 80-81 5.7738987442925016E-5 82-83 0.0 84-85 0.0 86-87 0.107798689555941 88-89 0.3742929860987614 90-91 0.27750800839755835 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.217373430365627E-5 100-101 3.031296840753563E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3463864.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.77393890827991 #Duplication Level Percentage of deduplicated Percentage of total 1 65.80597301132505 26.831687233636842 2 15.738519480799518 12.834428636337854 3 6.75452106928934 8.26225288301679 4 3.498433277905369 5.705796189920278 5 2.0714261881371145 4.223010242405692 6 1.358960828732796 3.3246111485697867 7 0.9171943129648665 2.6178337418695863 8 0.6350856277777953 2.0715954070830733 9 0.5004116716249355 1.836337943504281 >10 2.453715788042128 18.0827786376011 >50 0.17402998838114797 4.896868925328461 >100 0.08584726992656591 6.210825221902943 >500 0.0038265083769655965 1.0182735106074579 >1k 0.0017715316519955697 1.1899841878392752 >5k 2.8344506431929117E-4 0.8937160903764498 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9483 0.27376940896062896 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8201 0.236758718009714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7833 0.2261347443202158 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5415 0.15632830850171947 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3614 0.1043343503093655 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.8869493721462504E-5 0.0 0.0 0.0 0.0 6 2.8869493721462504E-5 0.0 0.0 0.0 0.0 7 5.773898744292501E-5 0.0 0.0 2.8869493721462504E-5 0.0 8 5.773898744292501E-5 0.0 0.0 8.660848116438752E-5 0.0 9 5.773898744292501E-5 0.0 0.0 1.1547797488585002E-4 0.0 10-11 5.773898744292501E-5 0.0 0.0 1.1547797488585002E-4 0.0 12-13 1.2991272174658127E-4 0.0 0.0 1.1547797488585002E-4 0.0 14-15 1.4434746860731253E-4 0.0 0.0 1.1547797488585002E-4 0.0 16-17 1.587822154680438E-4 0.0 0.0 1.4434746860731253E-4 0.0 18-19 1.7321696232877503E-4 0.0 0.0 1.8765170918950627E-4 0.0 20-21 1.7321696232877503E-4 0.0 0.0 2.3095594977170004E-4 0.0 22-23 1.7321696232877503E-4 0.0 0.0 3.608686715182813E-4 0.0 24-25 2.3095594977170004E-4 0.0 0.0 6.351288618721752E-4 0.0 26-27 2.4539069663243127E-4 0.0 0.0 0.0010681712676941127 0.0 28-29 2.8869493721462506E-4 0.0 0.0 0.0029013841190069817 0.0 30-31 2.8869493721462506E-4 0.0 0.0 0.008545370141552901 0.0 32-33 2.8869493721462506E-4 0.0 0.0 0.019559081996290847 0.0 34-35 3.4643392465755007E-4 0.0 0.0 0.03578373746775278 0.0 36-37 4.0417291210047507E-4 0.0 0.0 0.06525949055736599 0.0 38-39 4.907813932648625E-4 0.0 0.0 0.11038250924401188 0.0 40-41 5.196508869863251E-4 0.0 0.0 0.161885686043101 0.0 42-43 5.918246212899814E-4 0.0 0.0 0.21519320619978152 0.0 44-45 6.351288618721751E-4 0.0 0.0 0.28156417226542385 0.0 46-47 7.506068367580252E-4 0.0 0.0 0.3618646690516718 0.0 48-49 7.506068367580252E-4 0.0 0.0 0.45326548617382206 0.0 50-51 7.506068367580252E-4 0.0 0.0 0.5496318562160639 0.0 52-53 7.650415836187563E-4 0.0 0.0 0.6472251797414679 0.0 54-55 7.794763304794876E-4 0.0 0.0 0.7589645551903885 0.0 56-57 7.794763304794876E-4 0.0 0.0 0.8981876886621415 0.0 58-59 7.794763304794876E-4 0.0 0.0 1.049781400193541 0.0 60-61 7.794763304794876E-4 0.0 0.0 1.2168924646002268 0.0 62-63 7.794763304794876E-4 0.0 0.0 1.3863563927452116 0.0 64-65 8.083458242009501E-4 0.0 0.0 1.5725934967423663 0.0 66-67 8.660848116438752E-4 0.0 0.0 1.7932430372555044 0.0 68-69 8.660848116438752E-4 0.0 0.0 2.0453603259250364 0.0 70-71 8.949543053653377E-4 0.0 0.0 2.316242785513519 0.0 72-73 9.526932928082626E-4 0.0 0.0 2.591527842894525 0.0 74-75 9.81562786529725E-4 0.0 0.0 2.88813302138883 0.0 76-77 9.81562786529725E-4 0.0 0.0 3.210648570498149 0.0 78-79 9.81562786529725E-4 0.0 0.0 3.5797017434864644 0.0 80-81 0.0010681712676941127 0.0 0.0 3.982474485141449 0.0 82-83 0.0010970407614155752 0.0 0.0 4.3956402445361595 0.0 84-85 0.0011259102551370377 0.0 0.0 4.825030659402332 0.0 86-87 0.0011547797488585003 0.0 0.0 5.2761020640533225 0.0 88-89 0.0011547797488585003 0.0 0.0 5.770044666880686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14700 0.0 25.548004 1 TACATGG 14740 0.0 25.110964 2 ACATGGG 14730 0.0 24.161549 3 GAGTACT 10050 0.0 22.029366 12-13 GTATCAA 21990 0.0 21.667368 1 GTACTTT 10725 0.0 20.488026 14-15 CATGGGG 7420 0.0 20.145262 4 AGTACTT 10850 0.0 19.639616 12-13 CATGGGA 8170 0.0 19.05101 4 CTAGCGT 125 0.0060679293 18.981312 4 GGTATCA 17355 0.0 18.951813 1 ATCAACG 25155 0.0 18.883219 3 TCAACGC 25365 0.0 18.708174 4 CAACGCA 25355 0.0 18.696836 5 TATCAAC 25820 0.0 18.470392 2 AACGCAG 25880 0.0 18.280882 6 AGAGTAC 19525 0.0 18.071875 10-11 ACTTTTT 12995 0.0 17.017336 16-17 AGTACAT 11460 0.0 16.977179 2 CTAGACG 225 3.57092E-5 16.872278 3 >>END_MODULE