FastQCFastQC Report
Fri 27 May 2016
SRR939330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525900
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95720.2714767860688051No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75360.2137326640006807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72830.2065571910717831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.1364190703082901No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38950.11046824924132845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145100.027.6942041
TACATGG143600.027.080342
ACATGGG144850.025.9299323
GTATCAA203850.022.6020031
CATGGGA81250.021.7710884
GAGTACT98150.021.14984712-13
AATACCG1404.907438E-420.324415
CATGGGG71050.020.1575414
GTACTTT103250.020.12853614-15
GGTATCA157750.019.5115411
ATCAACG234500.019.353693
CAACGCA237100.019.2414685
TCAACGC237250.019.1493474
TATCAAC239750.019.028792
AACGCAG241100.018.909896
ATGGGAG37750.018.4669465
AGTACTT105850.018.33523612-13
AGAGTAC185450.017.8885910-11
CCTATAC8750.017.8854793
GTGTAGC14900.017.2144621
Produced by FastQC (version 0.11.5)