##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939330_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3525900 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.817678323264982 33.0 31.0 34.0 30.0 34.0 2 31.893848662752774 33.0 31.0 34.0 30.0 34.0 3 31.95146799398735 34.0 31.0 34.0 30.0 34.0 4 35.45293655520576 37.0 35.0 37.0 33.0 37.0 5 35.492382654074135 37.0 35.0 37.0 33.0 37.0 6 35.582426047250344 37.0 35.0 37.0 33.0 37.0 7 35.49732550554469 37.0 35.0 37.0 33.0 37.0 8 35.479818486060296 37.0 35.0 37.0 33.0 37.0 9 37.10236592075782 39.0 37.0 39.0 33.0 39.0 10-11 37.00674877336283 39.0 37.0 39.0 33.0 39.0 12-13 36.9030368132959 39.0 37.0 39.0 33.0 39.0 14-15 38.13873762727247 40.0 38.0 41.0 33.0 41.0 16-17 38.07398068578236 40.0 38.0 41.0 33.0 41.0 18-19 38.004957741285914 40.0 38.0 41.0 33.0 41.0 20-21 37.89906690490371 40.0 38.0 41.0 32.5 41.0 22-23 37.824033012847785 40.0 38.0 41.0 32.0 41.0 24-25 37.850078136078736 40.0 38.0 41.0 32.0 41.0 26-27 37.772277290904455 40.0 38.0 41.0 32.0 41.0 28-29 37.641169630448964 40.0 37.5 41.0 32.0 41.0 30-31 37.48080334099095 40.0 37.0 41.0 32.0 41.0 32-33 37.293898153662894 40.0 37.0 41.0 31.0 41.0 34-35 36.99287401798122 40.0 36.5 41.0 30.5 41.0 36-37 36.75121798689696 40.0 36.0 41.0 30.0 41.0 38-39 36.43686420488386 39.5 35.5 41.0 30.0 41.0 40-41 36.321128506197 39.0 35.0 41.0 30.0 41.0 42-43 36.16255395785473 39.0 35.0 41.0 29.5 41.0 44-45 36.15327306503304 39.0 35.0 41.0 29.0 41.0 46-47 35.98597039053858 39.0 35.0 41.0 28.0 41.0 48-49 35.800907002467454 39.0 35.0 41.0 27.5 41.0 50-51 34.65982330752432 38.0 34.0 40.0 25.5 40.5 52-53 34.78172012819422 38.5 34.5 39.5 26.0 40.5 54-55 35.2907711506282 39.0 35.0 40.5 26.0 41.0 56-57 35.18453713945375 39.0 35.0 41.0 26.0 41.0 58-59 34.979390368416574 38.5 35.0 40.5 26.0 41.0 60-61 34.68123230948127 38.0 34.0 40.0 26.0 41.0 62-63 34.32722765818656 38.0 34.0 40.0 24.5 41.0 64-65 33.95770186335403 37.0 34.0 40.0 23.0 41.0 66-67 33.58932159165036 36.5 33.5 39.5 22.5 41.0 68-69 33.174354774667464 36.0 33.0 39.0 22.0 41.0 70-71 32.75933520519584 35.5 32.5 39.0 21.0 40.5 72-73 32.32402138461102 35.0 32.0 38.0 20.0 40.0 74-75 31.883712385490227 35.0 32.0 37.0 18.5 39.5 76-77 31.44389021242803 35.0 31.0 37.0 17.5 39.0 78-79 31.017832184690434 35.0 31.0 36.0 13.0 39.0 80-81 30.55865622961513 35.0 31.0 36.0 7.5 37.5 82-83 30.122272469440425 34.0 30.0 35.0 6.5 37.0 84-85 29.711677585864603 34.0 30.0 35.0 2.0 37.0 86-87 29.376850874953913 34.0 29.5 35.0 2.0 36.0 88-89 28.962830624805015 34.0 29.0 35.0 2.0 36.0 90-91 28.559261748773363 34.0 29.0 35.0 2.0 35.5 92-93 28.143862134490483 34.0 29.0 35.0 2.0 35.0 94-95 27.669372784253667 34.0 27.0 35.0 2.0 35.0 96-97 27.093044896338526 34.0 26.5 35.0 2.0 35.0 98-99 26.349478714654417 33.0 25.0 35.0 2.0 35.0 100-101 23.831620011911852 31.0 12.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3026030236679844 1101 2 0.16105875833051897 1101 3 0.31020940319133317 1101 4 0.35664450518855517 1101 5 0.3608553538669952 1101 6 0.3698593805961039 1101 7 0.3526082950497056 1101 8 0.3263128673822422 1101 9 0.3131000895472553 1101 10-11 0.36085271622213355 1101 12-13 0.34681894076442177 1101 14-15 0.41456588934329375 1101 16-17 0.4805950472628737 1101 18-19 0.4383133842323943 1101 20-21 0.49381788382312664 1101 22-23 0.5603720994032813 1101 24-25 0.542821490328329 1101 26-27 0.5008936510295783 1101 28-29 0.5397078611628672 1101 30-31 0.6206859094682855 1101 32-33 0.6044005639322023 1101 34-35 0.5399822769256701 1101 36-37 0.56450140319469 1101 38-39 0.6938869518539761 1101 40-41 0.583461305534918 1101 42-43 0.41477984522656897 1101 44-45 0.5227511298548961 1101 46-47 0.4959853787224304 1101 48-49 0.5315150950441137 1101 50-51 0.437024922840763 1101 52-53 0.5270599467918728 1101 54-55 0.48186805870359706 1101 56-57 0.5136204032805054 1101 58-59 0.4013469140880659 1101 60-61 0.5192981826910241 1101 62-63 0.6059530638858419 1101 64-65 0.5703181874823784 1101 66-67 0.501518297487614 1101 68-69 0.45260145519510786 1101 70-71 0.41671350084580183 1101 72-73 0.49318620611294506 1101 74-75 0.5757467379909791 1101 76-77 0.5008750050254953 1101 78-79 0.3068323893101841 1101 80-81 0.31249651647842214 1101 82-83 0.1085159577963033 1101 84-85 0.16560013203493185 1101 86-87 0.32297086335274017 1101 88-89 0.35172580969750555 1101 90-91 0.5311705267013771 1101 92-93 0.4526369103554373 1101 94-95 0.37690506695324544 1101 96-97 0.2641481936606702 1101 98-99 0.2160996549181462 1101 100-101 0.03557805197263875 1102 1 0.30079782815868583 1102 2 0.3293057910027528 1102 3 0.36250465205329263 1102 4 0.258806322191127 1102 5 0.2942153267187848 1102 6 0.2755225540053132 1102 7 0.2547779432674986 1102 8 0.3248819638005642 1102 9 0.36073415894788496 1102 10-11 0.33155242526236606 1102 12-13 0.44396054720769484 1102 14-15 0.6204529551877513 1102 16-17 0.5814281680824607 1102 18-19 0.5893135709767492 1102 20-21 0.553684211003258 1102 22-23 0.515098739791874 1102 24-25 0.559238528643057 1102 26-27 0.6269479032790528 1102 28-29 0.5958853847719681 1102 30-31 0.600871830549714 1102 32-33 0.6216188962046374 1102 34-35 0.5551898944892741 1102 36-37 0.4801412435985384 1102 38-39 0.7396707460576764 1102 40-41 0.7570366769654413 1102 42-43 0.5288415953626142 1102 44-45 0.648575766857995 1102 46-47 0.7077838072585791 1102 48-49 0.7596392981175697 1102 50-51 0.7130637134601656 1102 52-53 0.8286300050440545 1102 54-55 0.8555532679476414 1102 56-57 0.9094545983821831 1102 58-59 0.8149614817547928 1102 60-61 0.6078299703968142 1102 62-63 0.6930501328025471 1102 64-65 0.7280623557370305 1102 66-67 0.663470739923163 1102 68-69 0.617121933822915 1102 70-71 0.5852890429566528 1102 72-73 0.669259735199887 1102 74-75 0.5540293725724119 1102 76-77 0.47156728445128593 1102 78-79 0.30684263013008817 1102 80-81 0.3814576957389555 1102 82-83 0.3121254452131481 1102 84-85 0.3149370891459782 1102 86-87 0.10356993123732394 1102 88-89 0.035684669713528905 1102 90-91 0.08280072467048427 1102 92-93 0.23125166878253012 1102 94-95 0.25913262600361975 1102 96-97 0.16818608871033902 1102 98-99 0.1516917249091314 1102 100-101 0.07745296537747137 1103 1 0.26998011068806704 1103 2 0.3231500101578675 1103 3 0.3351275795752322 1103 4 0.2976725136244269 1103 5 0.352219633197727 1103 6 0.2005055060333376 1103 7 0.24828777137856406 1103 8 0.36993259816824065 1103 9 0.464549043250571 1103 10-11 0.38698202022325745 1103 12-13 0.35675673706060707 1103 14-15 0.5191946695548992 1103 16-17 0.5106708241186837 1103 18-19 0.5827966279000591 1103 20-21 0.5632050007712195 1103 22-23 0.49368945271755393 1103 24-25 0.46165127945126727 1103 26-27 0.4287978222091269 1103 28-29 0.5029630896870003 1103 30-31 0.4809910489186109 1103 32-33 0.4656888691229426 1103 34-35 0.3782304525401514 1103 36-37 0.5033880890768359 1103 38-39 0.6701681646782376 1103 40-41 0.5584851276003704 1103 42-43 0.4836097329915887 1103 44-45 0.44259446648287337 1103 46-47 0.4048438332375781 1103 48-49 0.20231989615206913 1103 50-51 0.25290551066871814 1103 52-53 0.18652426104190312 1103 54-55 0.34361948852073 1103 56-57 0.35705034667967084 1103 58-59 0.25810833634922403 1103 60-61 0.14885825868488922 1103 62-63 0.021631928633716768 1103 64-65 0.03947635613273093 1103 66-67 0.051282345601279644 1103 68-69 0.22169984673713827 1103 70-71 0.10912614834038692 1103 72-73 0.09865117534647538 1103 74-75 0.01359413078181504 1103 76-77 0.11425363480791262 1103 78-79 0.020862352832335063 1103 80-81 -0.13471299735416054 1103 82-83 -0.07151562740185824 1103 84-85 -0.11386100593970738 1103 86-87 -0.06153266112924527 1103 88-89 0.03731094472866303 1103 90-91 0.005772752461563613 1103 92-93 0.016768376591233647 1103 94-95 -0.04600268493427251 1103 96-97 -0.16611668008731328 1103 98-99 -0.3233407090317222 1103 100-101 -0.5810795396214736 1104 1 0.2330639414654172 1104 2 0.2468756738088942 1104 3 0.2492676652238508 1104 4 0.33636802565867896 1104 5 0.28844177294942597 1104 6 0.18961272578112442 1104 7 0.27653729261430726 1104 8 0.2601205566206346 1104 9 0.36241047714355545 1104 10-11 0.3601378653024412 1104 12-13 0.3265256836305639 1104 14-15 0.35589927139096744 1104 16-17 0.407090008693622 1104 18-19 0.2763894593691347 1104 20-21 0.38746311393589394 1104 22-23 0.42868454165515146 1104 24-25 0.4947020241734208 1104 26-27 0.5057927113876204 1104 28-29 0.40276588475150277 1104 30-31 0.5008062755565632 1104 32-33 0.4863115666752691 1104 34-35 0.5413446760557292 1104 36-37 0.5348187931571644 1104 38-39 0.5761729749080402 1104 40-41 0.5104544512347076 1104 42-43 0.5298699299029934 1104 44-45 0.6426475894439534 1104 46-47 0.707253246389925 1104 48-49 0.6056807097053394 1104 50-51 0.7065818883346608 1104 52-53 0.6668782006326879 1104 54-55 0.604199001508789 1104 56-57 0.7124717383258883 1104 58-59 0.8082835639707255 1104 60-61 0.7413665487831551 1104 62-63 0.5647673632117503 1104 64-65 0.5636328170722962 1104 66-67 0.6933004443863524 1104 68-69 0.7474811612168821 1104 70-71 0.7129892092789305 1104 72-73 0.6307939024661948 1104 74-75 0.582290820784845 1104 76-77 0.6308214416835476 1104 78-79 0.5681141021064455 1104 80-81 0.5138774238535468 1104 82-83 0.5241911433835256 1104 84-85 0.41995904007731966 1104 86-87 0.4382308873701888 1104 88-89 0.40928818248336896 1104 90-91 0.48629471462207974 1104 92-93 0.4703227163101893 1104 94-95 0.4260576777695597 1104 96-97 0.19022621480718271 1104 98-99 0.31793096163870516 1104 100-101 0.14122551845829534 1105 1 0.23388137001504816 1105 2 0.2581044443454985 1105 3 0.30145345737219387 1105 4 0.23110733118346616 1105 5 0.307249908316777 1105 6 0.1845518000819908 1105 7 0.27451675518267393 1105 8 0.2792023352242765 1105 9 0.3389687417773857 1105 10-11 0.3775167445408272 1105 12-13 0.36314071495488776 1105 14-15 0.4042034756935635 1105 16-17 0.40460137548353714 1105 18-19 0.47074475221899803 1105 20-21 0.4260730228799545 1105 22-23 0.40971295743218406 1105 24-25 0.3097462382866283 1105 26-27 0.3220348287118284 1105 28-29 0.44808636428501103 1105 30-31 0.46744598636818324 1105 32-33 0.3029797658669082 1105 34-35 0.3824209305671573 1105 36-37 0.3545690720521364 1105 38-39 0.5884210895337887 1105 40-41 0.4927873252405206 1105 42-43 0.39841563495722454 1105 44-45 0.494572047694831 1105 46-47 0.6432803409400165 1105 48-49 0.6173595634773932 1105 50-51 0.4959047438654025 1105 52-53 0.5334344180097119 1105 54-55 0.7136070033898392 1105 56-57 0.6351426474147956 1105 58-59 0.7051707385610086 1105 60-61 0.6940905796714816 1105 62-63 0.7517258582587232 1105 64-65 0.7255561346823569 1105 66-67 0.6998369705066665 1105 68-69 0.6673144728675879 1105 70-71 0.50578673502811 1105 72-73 0.6717384396811212 1105 74-75 0.8424439522769624 1105 76-77 0.70914403260036 1105 78-79 0.6711392603312021 1105 80-81 0.63111489311245 1105 82-83 0.39400072085994253 1105 84-85 0.5068193921022974 1105 86-87 0.3606864290971892 1105 88-89 0.35421084740363185 1105 90-91 0.4577700331077956 1105 92-93 0.48264808677890514 1105 94-95 0.580779433272923 1105 96-97 0.5626322243443411 1105 98-99 0.2947296084959383 1105 100-101 0.06912448753273992 1106 1 0.12985240334239023 1106 2 0.2233116107184614 1106 3 0.08431091370611554 1106 4 0.16753180892937536 1106 5 0.15361925466299198 1106 6 0.19468997598844595 1106 7 0.22247568455868816 1106 8 0.23240986105736994 1106 9 0.27996605575233957 1106 10-11 0.26705245782635245 1106 12-13 0.27140367173778657 1106 14-15 0.3531656320572978 1106 16-17 0.39814062026986363 1106 18-19 0.34053975891367116 1106 20-21 0.34798088587530174 1106 22-23 0.3714401137232528 1106 24-25 0.40054709879306216 1106 26-27 0.4410865877799566 1106 28-29 0.4772538595888136 1106 30-31 0.30141586368025486 1106 32-33 0.36079165158977844 1106 34-35 0.3934129572168388 1106 36-37 0.38013850971682217 1106 38-39 0.6252644677216423 1106 40-41 0.6046261304148999 1106 42-43 0.46713601893437584 1106 44-45 0.5156032126044323 1106 46-47 0.5232037305568653 1106 48-49 0.6612511873119402 1106 50-51 0.5724884954167706 1106 52-53 0.552958482518271 1106 54-55 0.6198652786831218 1106 56-57 0.5875371339912192 1106 58-59 0.6540424981467012 1106 60-61 0.7183630008197923 1106 62-63 0.7628371288935583 1106 64-65 0.7334212304302739 1106 66-67 0.6148339111960937 1106 68-69 0.5881395685729629 1106 70-71 0.6062881003739591 1106 72-73 0.48738302897311314 1106 74-75 0.4320715157789259 1106 76-77 0.4570247599009356 1106 78-79 0.4515244090977113 1106 80-81 0.4723321105436433 1106 82-83 0.4345401308419454 1106 84-85 0.5133434693727565 1106 86-87 0.4627624265547361 1106 88-89 0.48422051276893896 1106 90-91 0.46891896906682007 1106 92-93 0.5929369417069665 1106 94-95 0.5098665286188364 1106 96-97 0.5050245289906954 1106 98-99 0.6027175463752705 1106 100-101 0.4392447010676541 1107 1 0.1127622050785213 1107 2 0.09532409010326859 1107 3 0.05854031075431365 1107 4 0.08065641368292376 1107 5 0.12201363699163181 1107 6 0.0908699844152423 1107 7 0.0927814426453395 1107 8 0.08000222963602965 1107 9 0.17959163490241536 1107 10-11 0.21100630748090055 1107 12-13 0.1730759663807362 1107 14-15 0.2561556071417641 1107 16-17 0.2808676591049917 1107 18-19 0.24202518064789302 1107 20-21 0.2965138908181686 1107 22-23 0.27745231164515616 1107 24-25 0.093452405154828 1107 26-27 0.14633956217375044 1107 28-29 0.16104844943438934 1107 30-31 0.20096522397592054 1107 32-33 0.21764743073487836 1107 34-35 0.24329462573642502 1107 36-37 0.35207958946626405 1107 38-39 0.4301461657204939 1107 40-41 0.25979404644701987 1107 42-43 0.3348655455631686 1107 44-45 0.5142206777789511 1107 46-47 0.39493359350562685 1107 48-49 0.5077559846137945 1107 50-51 0.3733781027452707 1107 52-53 0.36262282818410796 1107 54-55 0.3948199254740956 1107 56-57 0.3326591913944412 1107 58-59 0.42323672557674996 1107 60-61 0.26675774373538985 1107 62-63 0.4253987010390219 1107 64-65 0.4309898147957867 1107 66-67 0.13702081811279498 1107 68-69 0.41134429626102786 1107 70-71 0.273279722537616 1107 72-73 0.3588364704039222 1107 74-75 0.40841303811040675 1107 76-77 0.3282227998117584 1107 78-79 0.3153326380167698 1107 80-81 0.34616076902088366 1107 82-83 0.3974060828115924 1107 84-85 0.2930508573295434 1107 86-87 0.2773819803993014 1107 88-89 0.24280639155139383 1107 90-91 0.21526967098521865 1107 92-93 0.3315104579963126 1107 94-95 0.2681665012501142 1107 96-97 0.23250264789911768 1107 98-99 0.40938457704677944 1107 100-101 0.35607993359198176 1108 1 0.03499772970278059 1108 2 0.09708077779415447 1108 3 0.02356227760710894 1108 4 0.11632404335175295 1108 5 0.16779452331141442 1108 6 0.1189313281211497 1108 7 0.022153696788741684 1108 8 0.04807049242580774 1108 9 0.09274549039264457 1108 10-11 0.0827886238547677 1108 12-13 0.1469118800589655 1108 14-15 0.16647829405818015 1108 16-17 0.1976695148726364 1108 18-19 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#Quality Count 2 10733.0 3 3371.0 4 3168.0 5 3703.0 6 4008.0 7 4898.0 8 6129.0 9 7671.0 10 9145.0 11 11410.0 12 14304.0 13 19525.0 14 17941.0 15 18922.0 16 22713.0 17 28628.0 18 25062.0 19 19963.0 20 20153.0 21 21738.0 22 23531.0 23 26388.0 24 29685.0 25 33824.0 26 39951.0 27 46050.0 28 54322.0 29 64740.0 30 77850.0 31 95853.0 32 118274.0 33 152589.0 34 207460.0 35 298545.0 36 463632.0 37 722570.0 38 681141.0 39 116266.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.732841606338525 18.699961203399994 13.924082676578573 23.64311451368291 2 15.14833759953888 20.128397880100458 40.315798589667686 24.40746593069297 3 19.434703430007676 24.082920840930758 29.496470091117967 26.985905637943603 4 12.731433231878222 16.015500538702216 37.00571870600149 34.24734752341807 5 13.858490528529147 37.44234219753119 34.06009191169162 14.639075362248047 6 32.457977738155 35.6792564363283 17.377866482849274 14.48489934266742 7 29.05720973952846 32.42543684382606 20.577041404802557 17.94031201184292 8 26.242237038504516 35.1979294417366 19.754996805552523 18.804836714206367 9 26.517639439141306 16.940338305543282 20.423155813603007 36.11886644171241 10-11 25.17690944994741 26.364077292415555 27.22699864965884 21.232014607978194 12-13 25.892549760644286 23.737241433528872 27.83410252036588 22.53610628546096 14-15 22.680722361570165 25.24099247521111 26.106212647798216 25.972072515420507 16-17 22.280558358130804 28.584337648589702 26.707137272264152 22.42796672101534 18-19 22.194302337957215 27.25890658814347 28.819838932841378 21.726952141057936 20-21 22.89798084170315 26.484268463656385 28.53653562623227 22.0812150684082 22-23 22.537175446648938 26.394091667880136 28.613235983294715 22.45549690217621 24-25 22.442771646962704 26.52727696995607 28.729260594746787 22.30069078833444 26-27 22.307645904524666 26.6361923416758 28.767578270543027 22.28858348325651 28-29 22.030681059338903 26.991852494743256 28.89896908972333 22.07849735619451 30-31 22.25687550746957 26.583748569576247 28.596652648762294 22.562723274191885 32-33 22.008825987048255 26.8310886140907 28.61937561875006 22.54070978011099 34-35 22.605160995011865 26.706251448180375 28.412979107821386 22.275608448986382 36-37 22.61606683336294 26.47582847135876 28.499340729911047 22.408763965367257 38-39 22.215635797996676 26.867478931189353 28.55099172956338 22.36589354125059 40-41 22.393653102429454 26.727838326311073 28.54719655738339 22.331312013876083 42-43 22.24504057712856 26.751847496990745 28.267041917295828 22.736070008584868 44-45 22.30477536385029 26.750960815246668 28.23112507711783 22.713138743785212 46-47 22.602924066081474 27.16199069906457 27.858941742840166 22.376143492013792 48-49 22.61825306033211 27.0351861507206 27.7726464433732 22.573914345574092 50-51 22.292880037803094 27.23604729941303 27.96362875540146 22.50744390738242 52-53 22.645394006738254 27.26080249000229 27.576835110649135 22.516968392610327 54-55 22.288731146626915 27.050263185495858 27.747750645280146 22.913255022597077 56-57 22.449010370796156 27.249754211135123 27.68163121283822 22.6196042052305 58-59 22.621710741108135 26.92986760098574 27.70096820042242 22.747453457483697 60-61 22.436010646572527 27.095862665016046 28.049208119220225 22.418918569191206 62-63 22.26307779547817 27.12330833380173 28.113096258750485 22.50051761196961 64-65 22.14200778481281 27.167120681109914 27.90401046249691 22.78686107158037 66-67 22.145224546687515 27.194877395242482 27.65196202913635 23.00793602893365 68-69 22.21211446930525 26.99887576162274 27.94657013335543 22.842439635716584 70-71 22.293957530166793 26.992303837546338 27.792613620185985 22.921125012100884 72-73 22.33181580345293 26.816224778135272 27.92419619406694 22.927763224344854 74-75 22.131422079693994 27.441194887488564 27.535284775644016 22.89209825717343 76-77 22.268655866728814 26.99179864152769 27.638129734461003 23.10141575728249 78-79 22.330093495551466 27.0572343831751 27.715162352598337 22.897509768675096 80-81 22.377869443776476 27.2188966025558 27.54417018951526 22.85906376415246 82-83 22.337030413892954 27.02917011646245 27.712379105351854 22.92142036429274 84-85 22.310499493913873 27.017299720257903 27.601276999324835 23.070923786503382 86-87 22.275223331151718 26.98126925725537 27.68219030391366 23.06131710767925 88-89 22.314831117063104 26.86406456847954 27.581060379516025 23.24004393494133 90-91 22.20747157102645 26.93039122618296 27.73299116251709 23.1291460402735 92-93 22.426510574061133 26.74663195411292 27.740603381171287 23.08625409065466 94-95 22.2450026357549 26.549803240149235 27.8879809149422 23.317213209153664 96-97 22.248355383276948 26.44156747146078 27.999335447998163 23.310741697264103 98-99 22.247818080776565 26.559215306231682 27.60486536100742 23.58810125198434 100-101 22.29882557912323 25.960293043775394 28.236510014122196 23.504371362979178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2786.0 1 1587.5 2 445.0 3 564.0 4 505.0 5 479.0 6 849.5 7 1675.5 8 2702.0 9 3236.0 10 3102.5 11 2921.0 12 3128.5 13 3485.0 14 3812.5 15 4161.5 16 4413.0 17 4536.0 18 4862.0 19 5246.5 20 5695.5 21 6441.0 22 7453.0 23 9275.0 24 11556.5 25 14222.0 26 18659.0 27 23633.5 28 27705.5 29 33000.0 30 39938.5 31 46847.0 32 53857.0 33 59710.0 34 65757.0 35 72124.0 36 80718.5 37 91038.0 38 96253.0 39 98054.0 40 100464.0 41 105280.0 42 110104.5 43 115515.5 44 122868.5 45 129735.0 46 131568.0 47 136512.0 48 152517.5 49 152526.5 50 137083.5 51 128676.0 52 124204.5 53 122199.0 54 119111.5 55 111129.5 56 99259.5 57 88357.5 58 79944.5 59 69574.5 60 58426.5 61 48642.5 62 39702.0 63 31041.5 64 23902.0 65 18053.5 66 13359.5 67 10791.5 68 8416.0 69 5881.0 70 4173.0 71 3007.0 72 2276.0 73 1885.5 74 1467.0 75 1101.5 76 749.0 77 425.5 78 269.0 79 176.5 80 123.0 81 83.0 82 64.5 83 44.5 84 30.5 85 23.5 86 15.0 87 14.5 88 16.0 89 12.5 90 10.5 91 9.5 92 9.0 93 9.5 94 6.0 95 2.5 96 0.5 97 0.5 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28724580958053264 2 0.11443886667233896 3 0.12538642616069656 4 0.12688958847386483 5 0.13312913015116706 6 0.059927961655180245 7 0.06931563572421226 8 0.07371167645140247 9 0.07138602909895346 10-11 0.0665362035225049 12-13 0.07042173629427947 14-15 0.0665362035225049 16-17 0.06679145750021272 18-19 0.07161292152358263 20-21 0.0758529737088403 22-23 0.0834680507104569 24-25 0.09240193993023059 26-27 0.0925295669190845 28-29 0.08629002524178224 30-31 0.08240449247000767 32-33 0.07566862361382909 34-35 0.24263308658782154 36-37 0.3665447119884285 38-39 0.33858022065288296 40-41 0.32230068918573984 42-43 0.34486230465980316 44-45 0.3575682804390368 46-47 0.3633823988201594 48-49 0.3734791117161576 50-51 0.3675657278992598 52-53 0.3678918857596642 54-55 0.35461867891885757 56-57 0.3319719787855583 58-59 0.31718142885504413 60-61 0.2737173487620182 62-63 0.34508919708443236 64-65 0.373323123174225 66-67 0.3775489945829434 68-69 0.389815366289458 70-71 0.39025497036217704 72-73 0.37363510025809016 74-75 0.37725119827561754 76-77 0.36196432116622707 78-79 0.34004084063643325 80-81 0.338495135993647 82-83 0.3518817890467682 84-85 0.35917070818798036 86-87 0.35314387815876797 88-89 0.3680904166312147 90-91 0.39178649422842393 92-93 0.3864545222496384 94-95 0.38638361836694174 96-97 0.3902407895856377 98-99 0.3799313650415497 100-101 0.3583907654783176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3525900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.11040608638647 #Duplication Level Percentage of deduplicated Percentage of total 1 67.42279819749483 28.392014115769932 2 15.011098191728822 12.642468813126447 3 6.413408548348988 8.102137151066348 4 3.3571440928946474 5.654828041692286 5 1.9867663439110455 4.183176877042975 6 1.224736934172299 3.0944501788194896 7 0.8861575057117849 2.612151669541582 8 0.6294704931812233 2.1205806469807422 9 0.45771124024047793 1.7346965577147107 >10 2.363719860706868 17.85133833594676 >50 0.16265088277561573 4.711316012302313 >100 0.07866944629528022 5.903655211116708 >500 0.003913800328526709 1.0967367560639656 >1k 0.0015520242623130005 1.2073595162784543 >5k 2.0243794725821742E-4 0.6930901165371691 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9572 0.2714767860688051 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7536 0.2137326640006807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7283 0.2065571910717831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4810 0.1364190703082901 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3895 0.11046824924132845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.8361553078646587E-5 0.0 0.0 0.0 0.0 3 2.8361553078646587E-5 0.0 0.0 0.0 0.0 4 2.8361553078646587E-5 0.0 0.0 0.0 0.0 5 2.8361553078646587E-5 0.0 0.0 0.0 0.0 6 2.8361553078646587E-5 0.0 0.0 0.0 0.0 7 2.8361553078646587E-5 0.0 0.0 0.0 0.0 8 2.8361553078646587E-5 0.0 0.0 2.8361553078646587E-5 0.0 9 2.8361553078646587E-5 0.0 0.0 1.1344621231458635E-4 0.0 10-11 2.8361553078646587E-5 0.0 0.0 1.4180776539323293E-4 0.0 12-13 8.508465923593976E-5 0.0 0.0 1.4180776539323293E-4 0.0 14-15 8.508465923593976E-5 0.0 0.0 1.7016931847187953E-4 0.0 16-17 8.508465923593976E-5 0.0 0.0 1.7016931847187953E-4 0.0 18-19 9.926543577526306E-5 0.0 0.0 1.7016931847187953E-4 0.0 20-21 1.4180776539323293E-4 0.0 0.0 1.843500950112028E-4 0.0 22-23 1.4180776539323293E-4 0.0 0.0 2.8361553078646586E-4 0.0 24-25 2.268924246291727E-4 0.0 0.0 4.537848492583454E-4 0.0 26-27 2.268924246291727E-4 0.0 0.0 8.22485039280751E-4 0.0 28-29 3.6870019002240565E-4 0.0 0.0 0.0027794322017073653 0.0 30-31 3.970617431010522E-4 0.0 0.0 0.00860773135936924 1.4180776539323293E-5 32-33 3.970617431010522E-4 0.0 0.0 0.018973879009614565 2.8361553078646587E-5 34-35 4.1124251964037547E-4 0.0 0.0 0.03647295725913951 2.8361553078646587E-5 36-37 4.254232961796988E-4 0.0 0.0 0.06318954025922459 2.8361553078646587E-5 38-39 4.537848492583454E-4 0.0 0.0 0.10675288578802575 2.8361553078646587E-5 40-41 4.537848492583454E-4 0.0 0.0 0.15665503842990441 2.8361553078646587E-5 42-43 4.6796562579766866E-4 0.0 0.0 0.20694007203834483 2.8361553078646587E-5 44-45 4.82146402336992E-4 0.0 0.0 0.26927876570521003 2.8361553078646587E-5 46-47 5.81411838112255E-4 0.0 0.0 0.3496837686831731 2.8361553078646587E-5 48-49 5.955926146515784E-4 0.0 0.0 0.43683882129385404 2.8361553078646587E-5 50-51 5.955926146515784E-4 0.0 0.0 0.5322329050738819 2.8361553078646587E-5 52-53 6.097733911909016E-4 0.0 0.0 0.628435293116651 2.8361553078646587E-5 54-55 6.523157208088715E-4 0.0 0.0 0.7405343316600017 2.8361553078646587E-5 56-57 6.523157208088715E-4 0.0 0.0 0.8743583198615956 2.8361553078646587E-5 58-59 6.523157208088715E-4 0.0 0.0 1.025440313111546 2.8361553078646587E-5 60-61 6.523157208088715E-4 0.0 0.0 1.1912277716327746 2.8361553078646587E-5 62-63 6.664964973481948E-4 0.0 0.0 1.359468504495306 2.8361553078646587E-5 64-65 6.806772738875181E-4 0.0 0.0 1.5408689979863297 2.8361553078646587E-5 66-67 7.657619331234578E-4 0.0 0.0 1.758132675345302 2.8361553078646587E-5 68-69 7.941234862021044E-4 0.0 0.0 2.0018576817266514 2.8361553078646587E-5 70-71 8.22485039280751E-4 1.4180776539323293E-5 0.0 2.2688958847386482 2.8361553078646587E-5 72-73 8.366658158200744E-4 2.8361553078646587E-5 0.0 2.542187810204487 2.8361553078646587E-5 74-75 8.508465923593976E-4 2.8361553078646587E-5 0.0 2.8275050341756716 2.8361553078646587E-5 76-77 8.508465923593976E-4 2.8361553078646587E-5 0.0 3.140843472588559 2.8361553078646587E-5 78-79 8.508465923593976E-4 2.8361553078646587E-5 0.0 3.5063955302192347 2.8361553078646587E-5 80-81 9.926543577526306E-4 2.8361553078646587E-5 0.0 3.907966760259792 2.8361553078646587E-5 82-83 9.926543577526306E-4 2.8361553078646587E-5 0.0 4.3198332340678975 2.8361553078646587E-5 84-85 9.926543577526306E-4 2.8361553078646587E-5 0.0 4.741753878442384 2.8361553078646587E-5 86-87 0.0010068351342919538 2.8361553078646587E-5 0.0 5.1915964718227965 2.8361553078646587E-5 88-89 0.001021015910831277 2.8361553078646587E-5 0.0 5.678777049831249 2.8361553078646587E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14510 0.0 27.694204 1 TACATGG 14360 0.0 27.08034 2 ACATGGG 14485 0.0 25.929932 3 GTATCAA 20385 0.0 22.602003 1 CATGGGA 8125 0.0 21.771088 4 GAGTACT 9815 0.0 21.149847 12-13 AATACCG 140 4.907438E-4 20.32441 5 CATGGGG 7105 0.0 20.157541 4 GTACTTT 10325 0.0 20.128536 14-15 GGTATCA 15775 0.0 19.511541 1 ATCAACG 23450 0.0 19.35369 3 CAACGCA 23710 0.0 19.241468 5 TCAACGC 23725 0.0 19.149347 4 TATCAAC 23975 0.0 19.02879 2 AACGCAG 24110 0.0 18.90989 6 ATGGGAG 3775 0.0 18.466946 5 AGTACTT 10585 0.0 18.335236 12-13 AGAGTAC 18545 0.0 17.88859 10-11 CCTATAC 875 0.0 17.885479 3 GTGTAGC 1490 0.0 17.214462 1 >>END_MODULE