FastQCFastQC Report
Fri 27 May 2016
SRR939330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525900
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98160.2783970050199949No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82870.23503219036274425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79300.22490711591366744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52990.15028786976374825No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36550.10366147650245328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG144350.024.8591381
TACATGG145150.024.521582
ACATGGG145900.023.4522323
GTATCAA213800.022.6606081
GAGTACT103650.020.90260112-13
CATGGGG73150.019.91724
AGTACTT112400.019.88764812-13
ATCAACG247100.019.4746383
CAACGCA248050.019.4000555
TCAACGC248400.019.3536154
GGTATCA166150.019.2773021
CATGGGA79600.019.1976014
AACGCAG253700.018.9305956
GTACTTT113200.018.92954414-15
TATCAAC255200.018.8937112
AGAGTAC192500.018.74969910-11
GGACCGA3609.822543E-1118.4556676
ACGCAGA278850.017.189177
CGCAGAG281750.016.9280268
AGTACAT114550.016.8617842
Produced by FastQC (version 0.11.5)