##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3525900 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28702799285289 33.0 31.0 34.0 31.0 34.0 2 32.48771405882186 34.0 31.0 34.0 31.0 34.0 3 32.57997589267988 34.0 31.0 34.0 31.0 34.0 4 36.110469950934515 37.0 35.0 37.0 35.0 37.0 5 36.048327802830485 37.0 35.0 37.0 35.0 37.0 6 36.08611078022633 37.0 35.0 37.0 35.0 37.0 7 36.02967781275703 37.0 35.0 37.0 35.0 37.0 8 36.00941234862021 37.0 35.0 37.0 35.0 37.0 9 37.71678606880513 39.0 37.0 39.0 35.0 39.0 10-11 37.637366629796645 39.0 37.0 39.0 35.0 39.0 12-13 37.56109092713917 39.0 37.0 39.0 35.0 39.0 14-15 38.87017357270484 40.0 38.0 41.0 35.0 41.0 16-17 38.8200294960152 40.0 38.0 41.0 35.0 41.0 18-19 38.926188349073996 40.0 38.0 41.0 35.0 41.0 20-21 38.93159576278397 40.0 38.0 41.0 35.0 41.0 22-23 38.87423324541252 40.0 38.0 41.0 35.0 41.0 24-25 38.80942993278312 40.0 38.0 41.0 34.0 41.0 26-27 38.64686945177118 40.0 38.0 41.0 34.0 41.0 28-29 38.508178337445756 40.0 38.0 41.0 34.0 41.0 30-31 38.33716341926884 40.0 38.0 41.0 34.0 41.0 32-33 38.15486117019768 40.0 38.0 41.0 33.5 41.0 34-35 38.11591480189455 40.0 38.0 41.0 33.0 41.0 36-37 38.040970957769645 40.0 38.0 41.0 33.0 41.0 38-39 37.93934328823846 40.0 37.5 41.0 33.0 41.0 40-41 37.784414050313394 40.0 37.0 41.0 33.0 41.0 42-43 37.62045335942596 40.0 37.0 41.0 33.0 41.0 44-45 37.41303979125897 40.0 37.0 41.0 32.0 41.0 46-47 37.154304716526276 40.0 37.0 41.0 31.0 41.0 48-49 36.86743668283275 40.0 36.0 41.0 31.0 41.0 50-51 36.637401656314694 39.5 36.0 41.0 30.5 41.0 52-53 36.414758784991065 39.0 35.5 41.0 30.0 41.0 54-55 36.145681669928244 39.0 35.0 40.5 29.5 41.0 56-57 35.90153733798463 39.0 35.0 40.0 29.0 41.0 58-59 35.60979111716158 38.0 35.0 40.0 28.0 41.0 60-61 35.271297966476645 38.0 34.0 40.0 28.0 41.0 62-63 34.9010244192972 37.5 34.0 40.0 27.5 41.0 64-65 34.480766045548656 37.0 34.0 39.5 26.5 41.0 66-67 34.10755112169943 36.0 33.5 39.0 26.0 40.5 68-69 33.71951671913554 36.0 33.0 39.0 26.0 40.0 70-71 33.32672409881165 35.5 33.0 38.5 26.0 40.0 72-73 32.83973907371168 35.0 32.0 37.5 25.0 40.0 74-75 32.3425942312601 35.0 32.0 37.0 23.5 39.0 76-77 30.33551873280581 33.5 29.5 35.0 20.0 37.5 78-79 31.33239740208174 34.0 31.0 36.0 21.5 38.0 80-81 31.382759011883493 35.0 31.0 36.0 22.0 37.5 82-83 31.080469383703452 34.0 31.0 35.0 20.5 37.0 84-85 30.706168070563542 34.0 31.0 35.0 19.5 36.5 86-87 30.17640800930259 34.0 30.5 35.0 16.5 36.0 88-89 29.679548342267225 34.0 30.0 35.0 8.5 36.0 90-91 29.26074732692362 34.0 29.5 35.0 4.5 35.0 92-93 28.916654045775545 34.0 29.0 35.0 2.0 35.0 94-95 28.524248702458948 34.0 29.0 35.0 2.0 35.0 96-97 27.972875152443347 33.5 29.0 35.0 2.0 35.0 98-99 27.230080546810743 33.0 27.0 35.0 2.0 35.0 100-101 23.837216597180863 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21165505586483846 1101 2 0.19429438450798386 1101 3 0.1577552786516705 1101 4 0.14532547646752647 1101 5 0.12667136060878192 1101 6 0.18816093919108567 1101 7 0.1702900128424929 1101 8 0.18921687784175845 1101 9 0.17817765122266138 1101 10-11 0.24407032399413708 1101 12-13 0.24765725962378582 1101 14-15 0.2987984664003491 1101 16-17 0.26307727826473837 1101 18-19 0.2627056409463222 1101 20-21 0.2576897063398178 1101 22-23 0.30497739472954066 1101 24-25 0.2710893059102304 1101 26-27 0.2474091713597133 1101 28-29 0.21305656795914985 1101 30-31 0.18780604393648304 1101 32-33 0.2297236287044413 1101 34-35 0.3056626622070553 1101 36-37 0.33665190200132855 1101 38-39 0.40502379045038595 1101 40-41 0.35126678553844215 1101 42-43 0.3881558245914434 1101 44-45 0.5401866928127461 1101 46-47 0.4440567881849873 1101 48-49 0.46888494168127437 1101 50-51 0.44068460804632537 1101 52-53 0.49125917065176594 1101 54-55 0.4207108106823867 1101 56-57 0.44950138577223697 1101 58-59 0.37500729331335947 1101 60-61 0.46240388420446976 1101 62-63 0.48814534349082805 1101 64-65 0.308585509969987 1101 66-67 0.2868809335395355 1101 68-69 0.3843198325924888 1101 70-71 0.3745575858357384 1101 72-73 0.3165415206845026 1101 74-75 0.22907077139116439 1101 76-77 0.2800407838236971 1101 78-79 0.34572735789071274 1101 80-81 0.40863759755271545 1101 82-83 0.37801710099743957 1101 84-85 0.2838193988924722 1101 86-87 0.3305041131309139 1101 88-89 0.45311680054166814 1101 90-91 0.5369122653331893 1101 92-93 0.48164409272886743 1101 94-95 0.5794917243994071 1101 96-97 0.5375979324922184 1101 98-99 0.46918299967950716 1101 100-101 0.3566804293794412 1102 1 0.19237279678012698 1102 2 0.1912914146702107 1102 3 0.22364672194463964 1102 4 0.1133138782369798 1102 5 0.16589410245779135 1102 6 0.1792586546850501 1102 7 0.19292563844614818 1102 8 0.2347453543491156 1102 9 0.3152289376503603 1102 10-11 0.3297847818230437 1102 12-13 0.2798798990645892 1102 14-15 0.38677674783013316 1102 16-17 0.34793903723599584 1102 18-19 0.3005520969198301 1102 20-21 0.32143710346475984 1102 22-23 0.2536046228608697 1102 24-25 0.3119301977049247 1102 26-27 0.38544618690367116 1102 28-29 0.37896758929017693 1102 30-31 0.406405079693009 1102 32-33 0.30175113032590417 1102 34-35 0.37915831588260573 1102 36-37 0.3822673250777129 1102 38-39 0.31476160538070275 1102 40-41 0.4858537491754973 1102 42-43 0.40865121923449266 1102 44-45 0.448225937241034 1102 46-47 0.2988131470313462 1102 48-49 0.26179317389487977 1102 50-51 0.2598907637830905 1102 52-53 0.31557047551294914 1102 54-55 0.3613522072892579 1102 56-57 0.3400952930867973 1102 58-59 0.28417533713604115 1102 60-61 0.23522615377096656 1102 62-63 0.36851720573372404 1102 64-65 0.3917342408111253 1102 66-67 0.22366646065059825 1102 68-69 0.39951138612486403 1102 70-71 0.3115716237910249 1102 72-73 0.4503153431408151 1102 74-75 0.391350819663586 1102 76-77 0.4429255424716665 1102 78-79 0.3740749513984376 1102 80-81 0.4785624178865646 1102 82-83 0.4059980329923718 1102 84-85 0.42854668184231315 1102 86-87 0.60212158035527 1102 88-89 0.7317720202474156 1102 90-91 0.9413413195432732 1102 92-93 0.774166486992403 1102 94-95 0.6969627639631106 1102 96-97 0.4758557280653726 1102 98-99 0.3974207553737017 1102 100-101 0.6301072085210997 1103 1 0.10708496769853326 1103 2 0.11500587019600061 1103 3 0.13304267238947887 1103 4 0.12080403858146127 1103 5 0.12878585251322505 1103 6 0.060259431348058 1103 7 0.10396070213370479 1103 8 0.07162761167974452 1103 9 0.11857316655854788 1103 10-11 0.1682995840008843 1103 12-13 0.2300701635878184 1103 14-15 0.2040743387416981 1103 16-17 0.19768151632361963 1103 18-19 0.17298907913619388 1103 20-21 0.2122568352433376 1103 22-23 0.1456319442015115 1103 24-25 0.22940009952532847 1103 26-27 0.20950663763890987 1103 28-29 0.22078251260514747 1103 30-31 0.30918247597306703 1103 32-33 0.2640230626961113 1103 34-35 0.3618154627547199 1103 36-37 0.38079709921646554 1103 38-39 0.37863347947132553 1103 40-41 0.38179454046413497 1103 42-43 0.4067935575594035 1103 44-45 0.5210937265858249 1103 46-47 0.5262505094184817 1103 48-49 0.428916941176233 1103 50-51 0.5196571717345932 1103 52-53 0.38744862660116297 1103 54-55 0.31251966992603997 1103 56-57 0.3785989289922682 1103 58-59 0.2287890338728218 1103 60-61 0.09961227351718804 1103 62-63 0.24826779778501873 1103 64-65 0.36494782632306766 1103 66-67 0.3036476232884482 1103 68-69 0.2039784125803763 1103 70-71 0.3188671207777247 1103 72-73 0.4365173336433088 1103 74-75 0.3788881848771197 1103 76-77 0.42347921044285997 1103 78-79 0.4989268141754941 1103 80-81 0.46313922810650965 1103 82-83 0.5229776737528908 1103 84-85 0.5476614492275615 1103 86-87 0.5018855759142369 1103 88-89 0.7471960061966207 1103 90-91 0.8478203052556168 1103 92-93 0.6485905083711359 1103 94-95 0.554133998849327 1103 96-97 0.3215235129516145 1103 98-99 0.05172739355343836 1103 100-101 0.0689671898350106 1104 1 0.1622354455859849 1104 2 0.06794268170136064 1104 3 0.13295413412102874 1104 4 0.11990235875605038 1104 5 0.14838292619479176 1104 6 0.07788073878285218 1104 7 0.12723425831662638 1104 8 0.12338761712355506 1104 9 0.1325983335375298 1104 10-11 0.16182247119093063 1104 12-13 0.14871059509798812 1104 14-15 0.19303278751311126 1104 16-17 0.11405850369323645 1104 18-19 0.0804614897921212 1104 20-21 0.11582126775306278 1104 22-23 0.1077364692980538 1104 24-25 0.13494558770475606 1104 26-27 0.1101245280111911 1104 28-29 0.18122873213545887 1104 30-31 0.14367166734705705 1104 32-33 0.1525100666316348 1104 34-35 0.20683618109075042 1104 36-37 0.23183927755140843 1104 38-39 0.29112145437334647 1104 40-41 0.33501173903707837 1104 42-43 0.3547653640774584 1104 44-45 0.3249258515481017 1104 46-47 0.4134363629664648 1104 48-49 0.3713780744012567 1104 50-51 0.25074929556465264 1104 52-53 0.24635932189057286 1104 54-55 0.24716861140291257 1104 56-57 0.3790599665311589 1104 58-59 0.4589999207822899 1104 60-61 0.3259903302079934 1104 62-63 0.25068778025271854 1104 64-65 0.3275734310565994 1104 66-67 0.36000003914421796 1104 68-69 0.35786626232798113 1104 70-71 0.25533881645615963 1104 72-73 0.34851972999015857 1104 74-75 0.3282988573886456 1104 76-77 0.45026856503605117 1104 78-79 0.47158659715857354 1104 80-81 0.5396993362509086 1104 82-83 0.4991181829365665 1104 84-85 0.4882063817793174 1104 86-87 0.4412515866352784 1104 88-89 0.6844612106189949 1104 90-91 0.8205095374582321 1104 92-93 0.8234639291448254 1104 94-95 0.7891138485306008 1104 96-97 0.8158758388395349 1104 98-99 0.8806858499492449 1104 100-101 0.7630529383379141 1105 1 0.05081888392338385 1105 2 0.08866383205698725 1105 3 0.07200788870892438 1105 4 0.029071600003241826 1105 5 0.07654586424970233 1105 6 0.05800023519084618 1105 7 0.0874095853156831 1105 8 0.17385406367172607 1105 9 0.18142854094988792 1105 10-11 0.04311327121319408 1105 12-13 0.08424812623527345 1105 14-15 0.14636463807479316 1105 16-17 0.10619872364711824 1105 18-19 0.06495913977718004 1105 20-21 0.13524178351435978 1105 22-23 0.12192579245763824 1105 24-25 0.11210840119210275 1105 26-27 0.1542335603755376 1105 28-29 0.14081443627569712 1105 30-31 0.1862657573165265 1105 32-33 0.1654116081367718 1105 34-35 0.22276382981907972 1105 36-37 0.16681832772007255 1105 38-39 0.07363367986864233 1105 40-41 0.3048493442146665 1105 42-43 0.23748514382138097 1105 44-45 0.2672532821726321 1105 46-47 0.34671767337298576 1105 48-49 0.3841246435415968 1105 50-51 0.36691849664065046 1105 52-53 0.4168714898931398 1105 54-55 0.34262794875508007 1105 56-57 0.3225369290875548 1105 58-59 0.412787705200806 1105 60-61 0.2755770920668752 1105 62-63 0.21825073754557422 1105 64-65 0.3523759196068923 1105 66-67 0.36017233303373786 1105 68-69 0.38117813232661746 1105 70-71 0.34114789934776724 1105 72-73 0.2960738846315678 1105 74-75 0.3181839622409939 1105 76-77 0.3493837207436563 1105 78-79 0.279552250417769 1105 80-81 0.3400579378805304 1105 82-83 0.43059072997832715 1105 84-85 0.3959375218636474 1105 86-87 0.3681374768529295 1105 88-89 0.591323003647414 1105 90-91 0.5821400660190328 1105 92-93 0.43700620122028155 1105 94-95 0.4521708046522299 1105 96-97 0.43544484982101395 1105 98-99 0.6535393444931508 1105 100-101 0.7785175587551265 1106 1 0.05097690103637831 1106 2 0.06577022764675888 1106 3 0.11905223311426738 1106 4 0.10784051641529402 1106 5 0.06742686770356698 1106 6 0.052978605546087465 1106 7 0.07392956459887046 1106 8 0.028537520742297318 1106 9 0.11676657349769215 1106 10-11 0.11012475727595472 1106 12-13 0.12245631852304228 1106 14-15 0.12664440369544394 1106 16-17 0.08234172886850644 1106 18-19 0.048282042031317474 1106 20-21 0.05309499651717431 1106 22-23 0.07349514256151934 1106 24-25 0.1708870006943144 1106 26-27 0.15790185276622282 1106 28-29 0.15252786466390944 1106 30-31 0.11949240472691258 1106 32-33 0.14753356260728623 1106 34-35 0.14165518778074926 1106 36-37 0.09567730413785824 1106 38-39 0.21095117143249098 1106 40-41 0.18661346038054916 1106 42-43 0.2196666538162475 1106 44-45 0.2712323356439228 1106 46-47 0.3439204691503548 1106 48-49 0.26718395942587136 1106 50-51 0.2884259107716858 1106 52-53 0.32043939754804995 1106 54-55 0.24466943591324508 1106 56-57 0.2704661250135274 1106 58-59 0.35788260763786894 1106 60-61 0.2052838041517191 1106 62-63 0.40441997732995105 1106 64-65 0.3621354925370568 1106 66-67 0.3848715687732849 1106 68-69 0.34939732820651415 1106 70-71 0.3039651344329144 1106 72-73 0.4828366856129662 1106 74-75 0.3207382472786975 1106 76-77 0.3184833989665776 1106 78-79 0.5734525594329476 1106 80-81 0.5652725150168116 1106 82-83 0.4112103697595728 1106 84-85 0.46124241424362467 1106 86-87 0.5523627870999874 1106 88-89 0.8612568225709687 1106 90-91 0.812014065317836 1106 92-93 0.7045427773519144 1106 94-95 0.7572291336163879 1106 96-97 0.6434430503933868 1106 98-99 0.893209784427679 1106 100-101 0.9121284279219175 1107 1 0.044832054964714985 1107 2 0.03009424971912722 1107 3 0.052527015653417664 1107 4 -0.02289395905586389 1107 5 0.07367681376506141 1107 6 0.10018601764169688 1107 7 0.06243290741939944 1107 8 0.04949112627435426 1107 9 0.09634444921498897 1107 10-11 0.09222819429342621 1107 12-13 0.07302510172406329 1107 14-15 0.12768378278179426 1107 16-17 0.036546031798806666 1107 18-19 0.18164388907733553 1107 20-21 0.11938852404111344 1107 22-23 0.13139054004498263 1107 24-25 0.15835079353420412 1107 26-27 0.14301529367921972 1107 28-29 0.08807401419760907 1107 30-31 0.23051264279803974 1107 32-33 0.1281703848074116 1107 34-35 0.34448419753120874 1107 36-37 0.3004954807282232 1107 38-39 0.2540743381105628 1107 40-41 0.20196468104857246 1107 42-43 0.15284339670945712 1107 44-45 0.16398984407691586 1107 46-47 0.21079795956082847 1107 48-49 0.29560756809639344 1107 50-51 0.27558448640535715 1107 52-53 0.350854693749298 1107 54-55 0.2875835988717199 1107 56-57 0.3716792096033785 1107 58-59 0.2920903557971002 1107 60-61 0.21796061459090055 1107 62-63 0.2879432981204957 1107 64-65 0.35766934109958726 1107 66-67 0.3612791417487884 1107 68-69 0.441071136118417 1107 70-71 0.3783454653201517 1107 72-73 0.4581721532451155 1107 74-75 0.4613989041057849 1107 76-77 0.5090025510472032 1107 78-79 0.7305820001531806 1107 80-81 0.6017904891230721 1107 82-83 0.6277350189969013 1107 84-85 0.6136550870465918 1107 86-87 0.6902371833838572 1107 88-89 0.8763372612326954 1107 90-91 0.9342985016072056 1107 92-93 0.8782225612663339 1107 94-95 0.9125835380450802 1107 96-97 1.12531637824614 1107 98-99 1.1907499063487954 1107 100-101 0.9330179053707539 1108 1 -0.019885323577703673 1108 2 -0.010676080456583747 1108 3 -0.0035471225779843962 1108 4 0.03954804820504165 1108 5 -0.05926174097207593 1108 6 0.017274173210445554 1108 7 0.01740040506418694 1108 8 -0.08352867402999209 1108 9 -0.021351396775123987 1108 10-11 0.0266549300298351 1108 12-13 0.012573569576325383 1108 14-15 0.03129549502386908 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0.11014821301012745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 25.0 5 63.0 6 136.0 7 347.0 8 869.0 9 1531.0 10 2820.0 11 4402.0 12 6254.0 13 9060.0 14 10874.0 15 14623.0 16 18514.0 17 25592.0 18 24678.0 19 17677.0 20 16608.0 21 17747.0 22 19082.0 23 20889.0 24 24274.0 25 29350.0 26 35520.0 27 43706.0 28 53468.0 29 65518.0 30 80048.0 31 98504.0 32 121895.0 33 156795.0 34 213130.0 35 304922.0 36 486456.0 37 812672.0 38 718684.0 39 69151.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79785590222723 19.376741823472848 14.437421769924441 23.38798050437548 2 15.54797356703253 20.69426245781219 39.567826654187584 24.189937320967697 3 19.474091721262656 24.304007487450015 29.817408321279675 26.404492470007657 4 13.075923877591537 16.540315947701295 36.71326469837488 33.670495476332285 5 13.757451998071415 37.60625655860915 34.080461726084124 14.555829717235316 6 32.40999461130492 36.056070790436486 17.054170566380215 14.479764031878387 7 28.97708386511245 32.618281857114496 20.47843103888369 17.926203238889364 8 26.227119317053806 35.607362659179216 19.498964803312628 18.66655322045435 9 26.284976885334245 17.039167304801612 20.359539408378 36.31631640148614 10-11 25.18805127768797 26.5674721347741 27.07581043137922 21.16866615615871 12-13 25.87068549873791 23.84806716015769 27.830199381718142 22.451047959386255 14-15 22.60778498097255 25.40610679336978 26.08852815402909 25.897580071628575 16-17 22.17470211658039 28.76191618763678 26.734391680251946 22.32899001553089 18-19 22.069627612808077 27.361467993987347 28.900777106554354 21.668127286650215 20-21 22.824739782750502 26.488896451969712 28.720581979069177 21.965781786210613 22-23 22.497362375563686 26.463257607986613 28.732706543010295 22.306673473439407 24-25 22.37861741281722 26.589588408283056 28.856132212597245 22.175661966302478 26-27 22.22850438106129 26.683037150106443 28.91932970641472 22.169128762417543 28-29 22.02911313423523 26.997759437306783 29.015839927394428 21.957287501063558 30-31 22.22548852775178 26.638815621543436 28.696162681868458 22.439533168836327 32-33 21.992909611730337 26.862304659803172 28.715207464760773 22.42957826370572 34-35 22.55759606339981 26.76786592256728 28.527971158750955 22.146566855281954 36-37 22.590941610258103 26.509570875083043 28.604105783147162 22.295381731511693 38-39 22.186906538505337 26.90110122628086 28.682469568468093 22.22952266674571 40-41 22.341170300067994 26.783556921806113 28.66610539071699 22.209167387408904 42-43 22.240771656464478 26.77752648116098 28.336402215267604 22.64529964710694 44-45 22.34007613983569 26.79299159557569 28.297608455872446 22.569323808716177 46-47 22.58490854737228 27.221445666371658 27.896295310993956 22.297350475262107 48-49 22.560183215632886 27.125046087523753 27.826200402734052 22.488570294109305 50-51 22.324527071102413 27.279900167333164 27.985096003857173 22.41047675770725 52-53 22.655676564848694 27.315621543435718 27.530956635185344 22.497745256530248 54-55 22.272951586828896 27.10030063246263 27.722468022348906 22.904279758359568 56-57 22.478902192192376 27.290057473277386 27.660629578352985 22.570410756177253 58-59 22.653438203547367 26.964302832596214 27.675075342850384 22.707183621006035 60-61 22.459996865975054 27.189864478238025 27.953772422991264 22.396366232795657 62-63 22.275903487170833 27.234675869169227 28.01474253389783 22.47467810976211 64-65 22.085828323499907 27.228389128559837 27.84406189740546 22.8417206505348 66-67 22.145693298165007 27.268527184548624 27.58911199977311 22.99666751751326 68-69 22.19551652480614 27.112089148782957 27.837624128551138 22.854770197859768 70-71 22.283278352091433 27.15609165831301 27.65417732411261 22.90645266548295 72-73 22.31851442184974 26.948140900195693 27.760968830653166 22.9723758473014 74-75 22.131654329391075 27.587112510281063 27.33571570379194 22.945517456535917 76-77 22.208566215468856 27.12163010553944 27.521270845328928 23.148532833662777 78-79 22.2707535664653 27.22961513372472 27.54829972489293 22.95133157491704 80-81 22.304848265344702 27.409377402783264 27.375073705443125 22.910700626428905 82-83 22.260231430273123 27.250134717377122 27.51684676252872 22.97278708982104 84-85 22.218766839672142 27.20662809495448 27.389219773674807 23.185385291698573 86-87 22.139858448314385 27.207887465018782 27.390938227784705 23.261315858882128 88-89 22.12559352455531 27.099899981043645 27.33840562838603 23.436100866015018 90-91 22.011839372210137 27.195403722023098 27.40036902863494 23.392387877131824 92-93 22.192319691426302 27.091111489265153 27.40176976091211 23.31479905839644 94-95 21.950589332052527 26.91998933037919 27.50861943416692 23.620801903401357 96-97 21.91701409569188 26.811055333390055 27.612921523582628 23.659009047335434 98-99 21.868487478374316 27.032232905073883 27.1743242859979 23.924955330553903 100-101 21.721140550291565 26.580432284630184 27.83709211946075 23.8613350456175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 602.0 1 406.5 2 312.0 3 433.5 4 403.0 5 411.0 6 728.0 7 1534.0 8 2467.0 9 2929.5 10 2750.0 11 2519.0 12 2678.0 13 2946.5 14 3289.5 15 3526.5 16 3618.0 17 3845.0 18 4287.0 19 4655.5 20 5121.0 21 6099.0 22 7511.0 23 9491.0 24 11639.0 25 14321.5 26 18683.0 27 23808.0 28 28090.0 29 33312.5 30 40306.5 31 47449.0 32 54573.0 33 60317.0 34 66176.5 35 72557.5 36 81406.5 37 91850.5 38 96837.5 39 98270.5 40 100886.5 41 105839.5 42 110771.0 43 116320.0 44 123645.0 45 130318.0 46 131580.5 47 137188.0 48 153993.0 49 153837.0 50 138451.0 51 129635.0 52 125180.5 53 123485.0 54 120298.5 55 111549.5 56 98867.0 57 87912.5 58 79234.0 59 69037.0 60 58146.5 61 47882.5 62 39005.5 63 30480.0 64 23287.5 65 17442.5 66 12603.5 67 10163.5 68 7796.0 69 5313.5 70 3702.0 71 2586.5 72 2009.0 73 1713.0 74 1298.5 75 1026.5 76 707.5 77 356.0 78 215.0 79 126.5 80 67.0 81 39.0 82 18.0 83 8.5 84 6.0 85 3.5 86 1.5 87 2.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2787657052100173 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020562125982018778 16-17 6.80677273887518E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.508465923593975E-5 26-27 0.0011911852293031567 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003304120933662328 36-37 0.020590487535097422 38-39 0.006792591962335858 40-41 0.016988570294109304 42-43 0.0052752488726282645 44-45 5.388695084942851E-4 46-47 9.926543577526305E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.4180776539323295E-5 58-59 5.105079554156385E-4 60-61 0.0023398281289883434 62-63 0.001347173771235713 64-65 2.836155307864659E-5 66-67 0.0 68-69 5.388695084942851E-4 70-71 1.4180776539323295E-5 72-73 0.0 74-75 0.0 76-77 0.2301681840097564 78-79 0.0 80-81 0.00116282367622451 82-83 0.0 84-85 0.0 86-87 0.10360475339629598 88-89 0.356391275986273 90-91 0.35799370373521655 92-93 0.0 94-95 4.2542329617969875E-5 96-97 0.0 98-99 0.0 100-101 1.5598854193255623E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3525900.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.59723860105498 #Duplication Level Percentage of deduplicated Percentage of total 1 65.54415396164724 26.609116572852763 2 15.99377416335311 12.986061316820695 3 6.722931436547823 8.18797354884196 4 3.50650646202419 5.694179179797488 5 2.1177737826354033 4.298788377835412 6 1.338204831354804 3.2596452501357316 7 0.898282408747369 2.5527449695333138 8 0.6330775101222353 2.056095898511535 9 0.49187381993329615 1.797184694649994 >10 2.485265968785983 18.27373089841213 >50 0.1742927743545781 4.876655989753624 >100 0.08812624127244896 6.3494155100839915 >500 0.00377778680741703 1.023672676917704 >1k 0.001679016354805179 1.1448615689129245 >5k 2.7983605913419656E-4 0.8898735469407034 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9816 0.2783970050199949 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8287 0.23503219036274425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7930 0.22490711591366744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5299 0.15028786976374825 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3655 0.10366147650245328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.8361553078646587E-5 0.0 0.0 0.0 0.0 7 2.8361553078646587E-5 0.0 0.0 0.0 0.0 8 5.6723106157293173E-5 0.0 0.0 0.0 0.0 9 5.6723106157293173E-5 0.0 0.0 0.0 0.0 10-11 8.508465923593976E-5 0.0 0.0 2.8361553078646587E-5 0.0 12-13 1.4180776539323293E-4 0.0 0.0 5.6723106157293173E-5 0.0 14-15 1.7016931847187953E-4 0.0 0.0 8.508465923593976E-5 0.0 16-17 1.843500950112028E-4 0.0 0.0 8.508465923593976E-5 0.0 18-19 2.127116480898494E-4 0.0 0.0 8.508465923593976E-5 0.0 20-21 2.268924246291727E-4 0.0 0.0 9.926543577526306E-5 0.0 22-23 2.268924246291727E-4 0.0 0.0 2.2689242462917267E-4 0.0 24-25 2.5525397770781926E-4 0.0 0.0 3.828809665617289E-4 0.0 26-27 2.5525397770781926E-4 0.0 0.0 7.657619331234578E-4 0.0 28-29 3.687001900224056E-4 0.0 0.0 0.0027085283190107492 0.0 30-31 3.970617431010522E-4 0.0 0.0 0.008692816018605179 0.0 32-33 4.396040727190221E-4 0.0 0.0 0.019271675316940358 0.0 34-35 5.388695084942851E-4 0.0 0.0 0.03692674210839786 0.0 36-37 5.672310615729317E-4 0.0 0.0 0.06382767520349414 0.0 38-39 6.239541677302249E-4 0.0 0.0 0.10791570946425026 0.0 40-41 6.239541677302249E-4 0.0 0.0 0.15845599705039848 0.0 42-43 6.948580504268413E-4 0.0 0.0 0.20913809240193992 0.0 44-45 7.799427096627811E-4 0.0 0.0 0.2720014748007601 1.4180776539323293E-5 46-47 9.217504750560141E-4 0.0 0.0 0.3529737088402961 2.8361553078646587E-5 48-49 9.359312515953373E-4 0.0 0.0 0.44080943872486456 2.8361553078646587E-5 50-51 9.359312515953373E-4 0.0 0.0 0.5368274766726226 2.8361553078646587E-5 52-53 9.359312515953373E-4 0.0 0.0 0.6336679996596614 2.8361553078646587E-5 54-55 9.64292804673984E-4 0.0 0.0 0.7465895232422928 2.8361553078646587E-5 56-57 0.001021015910831277 0.0 0.0 0.8811650926004708 2.8361553078646587E-5 58-59 0.001021015910831277 0.0 0.0 1.032856859241612 2.8361553078646587E-5 60-61 0.001021015910831277 0.0 0.0 1.1999347684279191 2.8361553078646587E-5 62-63 0.001021015910831277 0.0 0.0 1.368941263223574 2.8361553078646587E-5 64-65 0.001021015910831277 0.0 0.0 1.552057630675856 2.8361553078646587E-5 66-67 0.0011061005700672168 0.0 0.0 1.7700303468617942 2.8361553078646587E-5 68-69 0.0011061005700672168 0.0 0.0 2.0147054652712786 2.8361553078646587E-5 70-71 0.0011202813466065402 0.0 0.0 2.281559318188264 2.8361553078646587E-5 72-73 0.00116282367622451 0.0 0.0 2.555928982671091 2.8361553078646587E-5 74-75 0.0011911852293031567 0.0 0.0 2.842990442156612 2.8361553078646587E-5 76-77 0.0011911852293031567 0.0 0.0 3.1576902351172746 2.8361553078646587E-5 78-79 0.0011911852293031567 0.0 0.0 3.524873082049973 2.8361553078646587E-5 80-81 0.0012762698885390965 0.0 0.0 3.9273235202359684 2.8361553078646587E-5 82-83 0.0012762698885390965 0.0 0.0 4.3397146827760285 2.8361553078646587E-5 84-85 0.0013613545477750362 0.0 0.0 4.763081766357526 2.8361553078646587E-5 86-87 0.0014748007600896224 0.0 0.0 5.214484245157265 2.8361553078646587E-5 88-89 0.001503162313168269 0.0 0.0 5.70513911341785 2.8361553078646587E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14435 0.0 24.859138 1 TACATGG 14515 0.0 24.52158 2 ACATGGG 14590 0.0 23.452232 3 GTATCAA 21380 0.0 22.660608 1 GAGTACT 10365 0.0 20.902601 12-13 CATGGGG 7315 0.0 19.9172 4 AGTACTT 11240 0.0 19.887648 12-13 ATCAACG 24710 0.0 19.474638 3 CAACGCA 24805 0.0 19.400055 5 TCAACGC 24840 0.0 19.353615 4 GGTATCA 16615 0.0 19.277302 1 CATGGGA 7960 0.0 19.197601 4 AACGCAG 25370 0.0 18.930595 6 GTACTTT 11320 0.0 18.929544 14-15 TATCAAC 25520 0.0 18.893711 2 AGAGTAC 19250 0.0 18.749699 10-11 GGACCGA 360 9.822543E-11 18.455667 6 ACGCAGA 27885 0.0 17.18917 7 CGCAGAG 28175 0.0 16.928026 8 AGTACAT 11455 0.0 16.861784 2 >>END_MODULE