FastQCFastQC Report
Fri 27 May 2016
SRR939329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962679
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94850.3201494323212201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69880.2358676049615905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.1605303848307562No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35240.11894639952556452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147300.028.7038171
GTACATG137100.028.5895461
TACATGG136900.028.055342
GGTATCA104600.027.2197571
ACATGGG136900.026.7045273
CATGGGG68550.024.6250154
ATCAACG173900.023.9947973
TCAACGC176200.023.7354034
TATCAAC177200.023.7084942
CAACGCA176600.023.6275445
AACGCAG181250.023.0327326
TAGTCCG1050.002219696822.5791365
GTATCGC1302.991344E-421.8703376
GTAAGGT12800.021.8562684
TAAGGTG14300.021.5528135
GAGTACT94900.020.8460212-13
CATGGGA65600.020.8172134
ACGCAGA202250.020.5474827
GTCCTAC9350.020.3007951
CGCAGAG204900.020.2589578
Produced by FastQC (version 0.11.5)