##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939329_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2962679 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.893206452673407 33.0 31.0 34.0 30.0 34.0 2 31.945990436358443 33.0 31.0 34.0 30.0 34.0 3 32.00025854977876 34.0 31.0 34.0 30.0 34.0 4 35.48816324684517 37.0 35.0 37.0 33.0 37.0 5 35.52368380104628 37.0 35.0 37.0 33.0 37.0 6 35.61491778218295 37.0 35.0 37.0 33.0 37.0 7 35.548006382061644 37.0 35.0 37.0 33.0 37.0 8 35.52907419264794 37.0 35.0 37.0 33.0 37.0 9 37.1608020983711 39.0 37.0 39.0 34.0 39.0 10-11 37.06207658676489 39.0 37.0 39.0 33.0 39.0 12-13 36.958368422633704 39.0 37.0 39.0 33.0 39.0 14-15 38.19011998937448 40.0 38.0 41.0 33.0 41.0 16-17 38.11582658803063 40.0 38.0 41.0 33.0 41.0 18-19 38.043372062920085 40.0 38.0 41.0 33.0 41.0 20-21 38.01034975439458 40.0 38.0 41.0 33.0 41.0 22-23 38.01485446111442 40.0 38.0 41.0 33.0 41.0 24-25 37.92654080985487 40.0 38.0 41.0 33.0 41.0 26-27 37.83778077206475 40.0 38.0 41.0 33.0 41.0 28-29 37.69262954238377 40.0 38.0 41.0 32.0 41.0 30-31 37.509195056231206 40.0 37.0 41.0 32.0 41.0 32-33 37.28438281703823 40.0 37.0 41.0 31.5 41.0 34-35 36.99477803704012 40.0 36.5 41.0 30.5 41.0 36-37 36.754464793519645 40.0 36.0 41.0 30.0 41.0 38-39 36.16064008284394 38.5 35.5 40.5 29.0 41.0 40-41 36.13111207795377 39.0 35.0 40.0 30.0 41.0 42-43 36.31901768635752 39.0 36.0 41.0 30.0 41.0 44-45 36.22492024954441 40.0 35.5 41.0 29.5 41.0 46-47 35.95397982704168 39.0 35.0 41.0 28.5 41.0 48-49 35.70038114152765 39.0 35.0 41.0 27.0 41.0 50-51 34.55049888968734 38.0 34.0 40.0 25.0 40.5 52-53 34.644902299574134 38.0 34.5 39.5 25.5 40.5 54-55 35.12699435207122 39.0 35.0 40.0 26.0 41.0 56-57 35.032041945819984 39.0 35.0 40.5 26.0 41.0 58-59 34.8098990812032 38.0 34.5 40.0 26.0 41.0 60-61 34.500975806018815 38.0 34.0 40.0 25.0 41.0 62-63 34.11151933773453 37.5 34.0 40.0 23.5 41.0 64-65 33.722164972985595 37.0 34.0 40.0 22.5 41.0 66-67 33.358050939706935 36.0 33.0 39.0 22.0 41.0 68-69 32.94865694190967 36.0 33.0 39.0 21.0 41.0 70-71 32.53730086857199 35.0 32.0 39.0 20.0 40.0 72-73 32.104952139600684 35.0 32.0 38.0 18.5 40.0 74-75 31.649794493429766 35.0 31.5 37.0 17.5 39.0 76-77 31.195586663286843 35.0 31.0 36.5 13.0 39.0 78-79 30.80859215595075 35.0 31.0 36.0 9.0 39.0 80-81 30.419376516996948 34.5 31.0 36.0 7.0 37.5 82-83 29.995365512092263 34.0 30.0 35.0 2.0 37.0 84-85 29.588114675940254 34.0 30.0 35.0 2.0 36.5 86-87 29.171464407720176 34.0 29.0 35.0 2.0 36.0 88-89 28.74777422731251 34.0 29.0 35.0 2.0 36.0 90-91 28.34108791401296 34.0 29.0 35.0 2.0 35.0 92-93 27.9006416152408 34.0 28.0 35.0 2.0 35.0 94-95 27.41945263054148 34.0 27.0 35.0 2.0 35.0 96-97 26.814960716297648 33.0 25.5 35.0 2.0 35.0 98-99 26.02732729397954 33.0 24.5 35.0 2.0 35.0 100-101 23.306827368067886 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34902147529301075 1101 2 0.4885917050309949 1101 3 0.47796002366830237 1101 4 0.37460466195910413 1101 5 0.28133600871604614 1101 6 0.33467144664634674 1101 7 0.266749735705055 1101 8 0.2773903671515612 1101 9 0.42916046253566975 1101 10-11 0.4595704298978802 1101 12-13 0.5262465383483388 1101 14-15 0.553321944058645 1101 16-17 0.5871749823958794 1101 18-19 0.5923455051633653 1101 20-21 0.5185426068267773 1101 22-23 0.6060132891176764 1101 24-25 0.6129413227773739 1101 26-27 0.6660243726247401 1101 28-29 0.7577320539713028 1101 30-31 0.7044530266322013 1101 32-33 0.7301119127160689 1101 34-35 0.5696759311542934 1101 36-37 0.5926824079462918 1101 38-39 0.8706167442537591 1101 40-41 0.8065846067568501 1101 42-43 0.6327507490821063 1101 44-45 0.6527474568720777 1101 46-47 0.7148529720182992 1101 48-49 0.7086044752152603 1101 50-51 0.6581799364430978 1101 52-53 0.6263355660904821 1101 54-55 0.6317992682429363 1101 56-57 0.7336295680179958 1101 58-59 0.7226731484469653 1101 60-61 0.6311911641849122 1101 62-63 0.591370303370951 1101 64-65 0.5472118546333391 1101 66-67 0.49709908871371766 1101 68-69 0.5819011626120059 1101 70-71 0.7153977872756059 1101 72-73 0.6779338955479588 1101 74-75 0.5733484377638547 1101 76-77 0.5338486632166628 1101 78-79 0.5230456465172644 1101 80-81 0.43598807184966404 1101 82-83 0.49119915008526505 1101 84-85 0.48278137832606305 1101 86-87 0.43921554411807406 1101 88-89 0.3652774194379411 1101 90-91 0.2921128679813947 1101 92-93 0.41931126554865017 1101 94-95 0.44920976123999523 1101 96-97 0.4747594338497656 1101 98-99 0.6494249397795677 1101 100-101 0.4197949399801537 1102 1 0.3891622080986288 1102 2 0.37165683694845697 1102 3 0.3685897393186863 1102 4 0.34933777970389457 1102 5 0.3483479744106148 1102 6 0.3742740456580833 1102 7 0.32330724845764536 1102 8 0.4184361418869358 1102 9 0.4624862298893362 1102 10-11 0.43257579615970343 1102 12-13 0.4105484008356868 1102 14-15 0.5806123070944622 1102 16-17 0.5300684475391932 1102 18-19 0.5295027153325975 1102 20-21 0.5585332065856079 1102 22-23 0.5951221870168695 1102 24-25 0.5444983120534985 1102 26-27 0.5532175700552102 1102 28-29 0.6575202344592839 1102 30-31 0.6870304041296365 1102 32-33 0.5213719777723327 1102 34-35 0.6031970599202836 1102 36-37 0.4616154205490872 1102 38-39 0.9059354911600153 1102 40-41 0.6534442924532087 1102 42-43 0.5890316708361993 1102 44-45 0.5453195493512482 1102 46-47 0.5520813191476748 1102 48-49 0.42737331164296677 1102 50-51 0.45733751625650143 1102 52-53 0.4443009568984593 1102 54-55 0.5368839834843087 1102 56-57 0.5111826464364384 1102 58-59 0.4263066752851117 1102 60-61 0.5139669183190492 1102 62-63 0.537465908876861 1102 64-65 0.2824967221435415 1102 66-67 0.38437660075158675 1102 68-69 0.4390465324418429 1102 70-71 0.439442917367181 1102 72-73 0.47047131294686295 1102 74-75 0.32276186494883774 1102 76-77 0.4435124014862559 1102 78-79 0.4257798128900845 1102 80-81 0.3933611748756931 1102 82-83 0.3757120782785357 1102 84-85 0.3240776260747644 1102 86-87 0.2905005601559978 1102 88-89 0.36728519592494635 1102 90-91 0.3642863813012873 1102 92-93 0.3125150894115265 1102 94-95 0.28267445226465426 1102 96-97 0.12298290832072922 1102 98-99 0.14577330763582452 1102 100-101 -0.3567680001762987 1103 1 0.37433237690942534 1103 2 0.4261718633668501 1103 3 0.4228268082877733 1103 4 0.32959409736449885 1103 5 0.31551998392968983 1103 6 0.41578516713349956 1103 7 0.33039405821621415 1103 8 0.3700110360848896 1103 9 0.3891038054759406 1103 10-11 0.40202644890381123 1103 12-13 0.4454597597630183 1103 14-15 0.5709588807991821 1103 16-17 0.4941702153319909 1103 18-19 0.5988593847502841 1103 20-21 0.6014996673998283 1103 22-23 0.6112174706094535 1103 24-25 0.5489564760036387 1103 26-27 0.5666576139979114 1103 28-29 0.630030491393093 1103 30-31 0.4944764172499987 1103 32-33 0.5744618505888326 1103 34-35 0.378113762482883 1103 36-37 0.4148654504585281 1103 38-39 0.696816580355339 1103 40-41 0.5546393989653424 1103 42-43 0.43547219913218527 1103 44-45 0.43478235099102136 1103 46-47 0.4065810247504018 1103 48-49 0.2869450499463042 1103 50-51 0.15758693195181195 1103 52-53 0.3310858623169395 1103 54-55 0.4293609380211407 1103 56-57 0.3535443011287427 1103 58-59 0.4114836825122765 1103 60-61 0.3011810966909181 1103 62-63 0.2791745017131291 1103 64-65 0.2615636388798137 1103 66-67 0.27373433944892867 1103 68-69 0.3087805028484851 1103 70-71 0.1326710063213028 1103 72-73 0.21551339200227204 1103 74-75 0.07962238529292165 1103 76-77 0.11417053896688145 1103 78-79 0.09870043006614182 1103 80-81 -0.11232659159804825 1103 82-83 -0.037264238144246065 1103 84-85 0.11521339447376633 1103 86-87 -0.035177638060819305 1103 88-89 -0.05007730838878288 1103 90-91 0.006351856582273996 1103 92-93 -0.13934826162248015 1103 94-95 -0.23798405822050483 1103 96-97 -0.13878234528505118 1103 98-99 0.01738984571723634 1103 100-101 -0.20127484536201123 1104 1 0.3062548286207125 1104 2 0.3416297222998459 1104 3 0.24523928214355806 1104 4 0.24930769789504836 1104 5 0.24297112455028724 1104 6 0.1698267236911164 1104 7 0.1682379850927731 1104 8 0.21283764378961223 1104 9 0.26906293371614964 1104 10-11 0.37198611066501996 1104 12-13 0.269069346662441 1104 14-15 0.25843579250956594 1104 16-17 0.31056518024454505 1104 18-19 0.3386951008063619 1104 20-21 0.35752963642183744 1104 22-23 0.44410302900670473 1104 24-25 0.5221135493100988 1104 26-27 0.4603674690760542 1104 28-29 0.5197517030395105 1104 30-31 0.5167698956650213 1104 32-33 0.4962218080152141 1104 34-35 0.4747517698427757 1104 36-37 0.40321220398330837 1104 38-39 0.8329735678932408 1104 40-41 0.7202842941487262 1104 42-43 0.4416845445377149 1104 44-45 0.5066549218665912 1104 46-47 0.4702517572071798 1104 48-49 0.484522526986332 1104 50-51 0.28899875680295395 1104 52-53 0.400016774050286 1104 54-55 0.46635096117864805 1104 56-57 0.41561068869864926 1104 58-59 0.378512355551031 1104 60-61 0.5062644711639024 1104 62-63 0.5183435866706034 1104 64-65 0.431124307982671 1104 66-67 0.48090283550728685 1104 68-69 0.36666341052437446 1104 70-71 0.48436203148525436 1104 72-73 0.4554312973043224 1104 74-75 0.4687660435855605 1104 76-77 0.416065348888214 1104 78-79 0.32819921073431146 1104 80-81 0.26978127554036746 1104 82-83 0.33979118523167173 1104 84-85 0.2710764187003427 1104 86-87 0.2683326599072764 1104 88-89 0.15751489788258866 1104 90-91 0.2832974691825534 1104 92-93 0.2973431923842931 1104 94-95 0.20223815664798295 1104 96-97 0.2383004970176259 1104 98-99 0.3889722892298586 1104 100-101 0.1357101802571279 1105 1 0.08281131163082378 1105 2 0.2255699037281964 1105 3 0.21751967094244407 1105 4 0.11009409288794814 1105 5 0.13283447215824395 1105 6 0.22243015692112778 1105 7 0.1956963764589048 1105 8 0.2722428100090326 1105 9 0.295827050535614 1105 10-11 0.3509896995481441 1105 12-13 0.31281525707585445 1105 14-15 0.32095774544247746 1105 16-17 0.37025070187191744 1105 18-19 0.29810540409235387 1105 20-21 0.42191056531905957 1105 22-23 0.35646159111608 1105 24-25 0.406498793343232 1105 26-27 0.36450877950320404 1105 28-29 0.4248803576610456 1105 30-31 0.32108804960878956 1105 32-33 0.37164043078050213 1105 34-35 0.15297317612758832 1105 36-37 0.21698763326548942 1105 38-39 0.5559478780654672 1105 40-41 0.45425089936323815 1105 42-43 0.2877566471197994 1105 44-45 0.16374526167712844 1105 46-47 0.2524529131755813 1105 48-49 0.058238340610984096 1105 50-51 0.018688689886246834 1105 52-53 0.0850831288970113 1105 54-55 0.20433057858277692 1105 56-57 0.27741428708534954 1105 58-59 0.3992311724332325 1105 60-61 0.3408773193526642 1105 62-63 0.2567429024133432 1105 64-65 0.27973791003594783 1105 66-67 0.14553946189293043 1105 68-69 0.09488825222245367 1105 70-71 0.1537018349539352 1105 72-73 0.11027185881138024 1105 74-75 0.11844905447164322 1105 76-77 0.20405867583874127 1105 78-79 0.10516190728329633 1105 80-81 0.08044653208225583 1105 82-83 0.004615956680368072 1105 84-85 0.23443603455050877 1105 86-87 0.19383652587093536 1105 88-89 0.025905628302819395 1105 90-91 0.08511239480104038 1105 92-93 0.15455958296573868 1105 94-95 0.23621291695566882 1105 96-97 0.17808027097385448 1105 98-99 0.2598871815216235 1105 100-101 -0.20873173846292303 1106 1 0.1433442096819526 1106 2 0.17428051952492396 1106 3 0.20092087046169382 1106 4 0.21454275620640573 1106 5 0.17854900300161347 1106 6 0.1530442230772806 1106 7 0.13586707219914018 1106 8 0.08911630171879636 1106 9 0.16701328986896158 1106 10-11 0.296613705479011 1106 12-13 0.36869468331877187 1106 14-15 0.44180392055410067 1106 16-17 0.3912501717017136 1106 18-19 0.38466393950266564 1106 20-21 0.41747799776269545 1106 22-23 0.4610200649298477 1106 24-25 0.36438454349740823 1106 26-27 0.4216507514577117 1106 28-29 0.3884522382546933 1106 30-31 0.1959059181778997 1106 32-33 0.2252938387923109 1106 34-35 0.1537801641081984 1106 36-37 0.17251951570703028 1106 38-39 0.6017403211968286 1106 40-41 0.5723782106083419 1106 42-43 0.48097197175189166 1106 44-45 0.31531765997500116 1106 46-47 0.2913333447620019 1106 48-49 0.31228336328070583 1106 50-51 0.13366797805024788 1106 52-53 0.2801842975446647 1106 54-55 0.21664108673221705 1106 56-57 0.3745828262134552 1106 58-59 0.39555902880770333 1106 60-61 0.3435598852591326 1106 62-63 0.2542003953022416 1106 64-65 0.44285635944576285 1106 66-67 0.2747182187134669 1106 68-69 0.260654802808979 1106 70-71 0.3080315949547554 1106 72-73 0.12850553407883325 1106 74-75 0.20458411368131735 1106 76-77 0.335451791545637 1106 78-79 0.436394542009225 1106 80-81 0.3667078275307887 1106 82-83 0.2525717215663832 1106 84-85 0.308661513078615 1106 86-87 0.4477022586167543 1106 88-89 0.43595140039709435 1106 90-91 0.3703358985734617 1106 92-93 0.37715608970872694 1106 94-95 0.4482707326397595 1106 96-97 0.5213379798906139 1106 98-99 0.5187061192306608 1106 100-101 0.3893992465757208 1107 1 0.15841621643373216 1107 2 0.1216121634787406 1107 3 0.22632112925109737 1107 4 0.24566949005114935 1107 5 0.2501432179652241 1107 6 0.22594745598319577 1107 7 0.2293484642650796 1107 8 0.1879457210358524 1107 9 0.2768047775683371 1107 10-11 0.33356421756769805 1107 12-13 0.3096201054184462 1107 14-15 0.3361257203198065 1107 16-17 0.26793545455556256 1107 18-19 0.37368428836838063 1107 20-21 0.32431650413303004 1107 22-23 0.2880858648505793 1107 24-25 0.34335180441324553 1107 26-27 0.420987570721131 1107 28-29 0.43389384402163955 1107 30-31 0.33080306386332126 1107 32-33 0.4272060886597231 1107 34-35 0.5053126026110277 1107 36-37 0.549974851330667 1107 38-39 0.9744850342307672 1107 40-41 0.666973475991874 1107 42-43 0.4822253109796364 1107 44-45 0.4403247469005436 1107 46-47 0.567745508868633 1107 48-49 0.5470568794938018 1107 50-51 0.47802216835494704 1107 52-53 0.5525937541467272 1107 54-55 0.604800191739038 1107 56-57 0.49346495551102976 1107 58-59 0.5510546076426621 1107 60-61 0.5493936868677949 1107 62-63 0.5533696640965857 1107 64-65 0.5546096397055038 1107 66-67 0.4917078578077465 1107 68-69 0.6307808221002418 1107 70-71 0.6363509733243333 1107 72-73 0.616650812105199 1107 74-75 0.6629499511836769 1107 76-77 0.6915391196921767 1107 78-79 0.6236254332059588 1107 80-81 0.6511062689573777 1107 82-83 0.6599415978388308 1107 84-85 0.6992462603881542 1107 86-87 0.7728483688933849 1107 88-89 0.7912091695214478 1107 90-91 0.7810675491776315 1107 92-93 0.8752606810078234 1107 94-95 0.8909454591442376 1107 96-97 0.9889979165496463 1107 98-99 1.0449785184007538 1107 100-101 0.8860540981115079 1108 1 0.05164506257839108 1108 2 0.1266166009726426 1108 3 0.10949252799331077 1108 4 0.06807452301782746 1108 5 0.14485892601111772 1108 6 0.08551254745517411 1108 7 0.18054638286317726 1108 8 0.14200679578527797 1108 9 0.22612975750804054 1108 10-11 0.2827995603581286 1108 12-13 0.2094198778674894 1108 14-15 0.2834192471753525 1108 16-17 0.3849966801411 1108 18-19 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-1.4023485566674552 2316 50-51 -1.525086851215704 2316 52-53 -1.5261071621534654 2316 54-55 -1.3995064428432897 2316 56-57 -1.3443774598230647 2316 58-59 -1.2493645908021094 2316 60-61 -1.2223855950278661 2316 62-63 -1.427180210845627 2316 64-65 -1.5967904622373368 2316 66-67 -1.6247074525844987 2316 68-69 -1.7088219327272185 2316 70-71 -1.5530412339752502 2316 72-73 -1.4172838259384477 2316 74-75 -1.3707455242137243 2316 76-77 -1.369215115084554 2316 78-79 -1.3331175641506405 2316 80-81 -1.1008025988843961 2316 82-83 -1.2721079511233668 2316 84-85 -1.2798312280247366 2316 86-87 -1.273390343307753 2316 88-89 -1.3894469630516646 2316 90-91 -1.2737303313595838 2316 92-93 -1.390369598386684 2316 94-95 -1.3917069987450716 2316 96-97 -1.1671547132200324 2316 98-99 -1.3503718449187865 2316 100-101 -0.5293791471084397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11135.0 3 2627.0 4 2638.0 5 2991.0 6 3639.0 7 4303.0 8 5321.0 9 6540.0 10 7534.0 11 9475.0 12 11978.0 13 15844.0 14 16073.0 15 16911.0 16 20399.0 17 25382.0 18 22473.0 19 17403.0 20 16932.0 21 18129.0 22 19813.0 23 22182.0 24 25166.0 25 28467.0 26 32607.0 27 38729.0 28 45546.0 29 54128.0 30 65379.0 31 79512.0 32 100035.0 33 129536.0 34 177932.0 35 258546.0 36 403691.0 37 617069.0 38 546381.0 39 80219.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35380404205492 18.428410970460135 13.786059913840088 23.431725073644856 2 15.403234150989716 19.944246704949155 40.36003616795511 24.29248297610602 3 19.758596144774685 24.110594683975336 29.23772987723537 26.893079294014612 4 12.86163149759696 15.669249527339879 36.81340832761181 34.65571064745136 5 13.799995873176629 37.505247323568994 33.89119866967451 14.80355813357986 6 32.73583028065906 35.26898957129201 17.359914605090783 14.635265542958148 7 29.211484251618568 32.552749482162355 20.2660142706558 17.969751995563275 8 26.75907141876942 34.6993048687579 19.697807527805587 18.84381618466709 9 26.917441147092973 16.711301752446815 20.118508603366305 36.252748497093904 10-11 25.4428476627698 26.29046210271717 27.00292192852352 21.263768305989505 12-13 26.13122011388406 23.585573559974993 27.682814321680212 22.60039200446074 14-15 22.834938547168818 25.0448340009707 26.061634668292612 26.058592783567875 16-17 22.289164972354296 28.389094992212975 26.813832211882705 22.507907823550024 18-19 22.291044850530064 26.863927405506338 29.072442562229778 21.77258518173382 20-21 23.016478896524863 26.06959334068794 29.02739991405725 21.886527848729944 22-23 22.648841175727753 25.989472472376523 29.00408750185323 22.35759885004249 24-25 22.40985678336984 26.217158738162365 29.177957713065556 22.19502676540224 26-27 22.11119765035527 26.43879476736431 29.0882988253636 22.361708756916823 28-29 21.9945057267233 26.811614048434134 29.067850048603187 22.126030176239382 30-31 22.24110363461272 26.39778656431149 28.92787892095686 22.43323088011892 32-33 22.032892584756684 26.483357183852423 28.881319084444364 22.60243114694653 34-35 22.56584343565813 26.657333670477378 28.638602530036845 22.138220363827653 36-37 22.71269085249298 26.1999948791175 28.79597708896821 22.291337179421312 38-39 22.27654004154635 26.56478120362374 28.770229691487405 22.388449063342506 40-41 22.54102306131968 26.49729767108874 28.733040953909907 22.228638313681675 42-43 22.239373088646133 26.502004786532744 28.554073460749297 22.704548664071826 44-45 22.304216640227153 26.74403720757382 28.54211053937114 22.409635612827884 46-47 22.651611380610806 26.972577266312342 27.946913043566717 22.428898309510135 48-49 22.555864690314344 26.934543390175847 27.93653339808433 22.57305852142548 50-51 22.500342922212695 27.178310564259213 27.934943629353054 22.386402884175034 52-53 22.76381613875376 27.040583002640624 27.76820022284136 22.427400635764258 54-55 22.2472473575548 27.051304786256335 27.97343534320678 22.728012512982083 56-57 22.39477371053977 27.33152532468996 27.731997600996344 22.541703363773927 58-59 22.510253052955285 26.88420552840481 27.851787360078 22.753754058561906 60-61 22.340924642453068 26.977212522780704 28.28563782578945 22.396225008976785 62-63 22.276456482954444 27.03745854716086 28.281713477840213 22.40437149204448 64-65 22.01742929068311 27.017536958441774 28.155610600814917 22.8094231500602 66-67 22.00969270266329 27.216485055670297 27.870810314012367 22.903011927654045 68-69 22.228617653992067 26.938559196278515 28.109782466756094 22.723040682973323 70-71 22.293283210408603 26.93617687323776 27.86148273799642 22.90905717835722 72-73 22.270017368697445 27.01401348494614 27.922737359622246 22.793231786734168 74-75 22.09924664742333 27.43437204622064 27.692990426151404 22.77339088020463 76-77 22.17558921360201 27.001325751796205 27.828360916430167 22.994724118171618 78-79 22.35790121744956 27.07978564534387 27.79445372413585 22.767859413070727 80-81 22.39112139433773 27.220418419970933 27.628922422377883 22.75953776331346 82-83 22.36507538527549 27.183199518684297 27.751786752324314 22.6999383437159 84-85 22.308199851763746 27.20582800390324 27.61183326744302 22.874138876889997 86-87 22.28941521358126 27.07511174689308 27.67850936781927 22.956963671706397 88-89 22.243409538157767 27.059011224304165 27.559826475373857 23.13775276216421 90-91 22.2106439303306 27.05801594729298 27.68002176002887 23.051318362347555 92-93 22.371434497141596 26.842166937801547 27.746434136777815 23.039964428279045 94-95 22.21588181357695 26.751486072138324 27.818822738171328 23.2138093761134 96-97 22.24354746891951 26.473153336418502 28.006213978670946 23.277085215991043 98-99 22.240095095897125 26.569485035707597 27.667835864617217 23.52258400377806 100-101 22.281878375367864 26.095418875374136 28.23040951496428 23.39229323429372 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3543.0 1 2086.5 2 717.0 3 779.0 4 673.5 5 669.0 6 1054.5 7 1915.5 8 2923.5 9 3379.5 10 3174.5 11 2980.0 12 3103.5 13 3356.0 14 3541.0 15 3785.5 16 4069.0 17 4224.0 18 4405.0 19 4686.0 20 5234.5 21 5887.0 22 6808.5 23 8261.5 24 9980.5 25 12289.0 26 15839.0 27 20474.5 28 24557.0 29 28192.5 30 32565.5 31 38813.5 32 46849.0 33 52193.0 34 55305.5 35 59443.5 36 65997.5 37 73054.0 38 77431.5 39 80500.0 40 83723.5 41 89589.0 42 94442.0 43 97804.0 44 102254.5 45 107562.5 46 109794.5 47 112476.0 48 120475.0 49 119449.5 50 111610.5 51 106650.0 52 103768.5 53 103900.5 54 102195.0 55 94133.5 56 83915.0 57 75980.0 58 70584.5 59 62610.0 60 51457.0 61 42338.5 62 35379.5 63 27238.0 64 19672.0 65 15044.5 66 11300.5 67 9109.5 68 7160.5 69 4901.0 70 3459.5 71 2425.5 72 1828.0 73 1464.0 74 1099.5 75 868.0 76 655.0 77 411.0 78 263.0 79 188.0 80 120.5 81 90.0 82 73.5 83 55.5 84 42.0 85 31.0 86 26.0 87 20.5 88 14.5 89 10.5 90 8.0 91 4.5 92 4.5 93 6.0 94 6.5 95 5.5 96 3.0 97 1.5 98 1.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29152668918907515 2 0.18108610483957255 3 0.1820649486495162 4 0.20282318806728641 5 0.21635823523236908 6 0.10976551965298975 7 0.12623709824790333 8 0.13842201601996032 9 0.14324872860002721 10-11 0.12979806452200865 12-13 0.11911516569969274 14-15 0.13457414724983707 16-17 0.11030557141020003 18-19 0.138641412046327 20-21 0.16525583770634616 22-23 0.1689180636849284 24-25 0.1920559061579064 26-27 0.17300220509883116 28-29 0.1705888488087977 30-31 0.1601084693954357 32-33 0.16949186867696434 34-35 0.3520293626140395 36-37 0.47148206066198867 38-39 0.4374925531925666 40-41 0.42633710908269173 42-43 0.45102760035764927 44-45 0.4554830273546341 46-47 0.4588077209849599 48-49 0.47075636611323735 50-51 0.46442763458342934 52-53 0.47067198302617325 54-55 0.45303591782977504 56-57 0.4147428729200835 58-59 0.40411060395000603 60-61 0.3584256006134988 62-63 0.46734728939584746 64-65 0.46554149133267564 66-67 0.4748742607619658 68-69 0.4858440620802996 70-71 0.47911029173258396 72-73 0.4719883591843733 74-75 0.4767981951470274 76-77 0.45259712577704164 78-79 0.4383870139154461 80-81 0.4351298267547716 82-83 0.44768603010991065 84-85 0.4500318799302928 86-87 0.451618281967098 88-89 0.4586220781934189 90-91 0.4777264091047326 92-93 0.48164178434450716 94-95 0.4799203693683993 96-97 0.47813144792264023 98-99 0.47512741002315806 100-101 0.45755885129641116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2962679.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.020966279670034 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17411447549478 35.87065798247945 2 13.912249951323735 13.639838714763638 3 5.192675591987231 7.6364992528821505 4 2.444177734619796 4.792638172412693 5 1.3577785303550276 3.3279807775896875 6 0.8026222013244603 2.3607189519864544 7 0.5746813325877818 1.9720003958439047 8 0.3926294116490024 1.5397658519081945 9 0.2960193058160721 1.3060037167686902 >10 1.6643213015806104 14.968795759607955 >50 0.12404423529275453 4.177197947503586 >100 0.058959228811757275 5.086277568149641 >500 0.004001789772432721 1.3901463780733623 >1k 0.0015869166338010996 1.3743173591344273 >5k 1.37992750765313E-4 0.5571611708961046 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9485 0.3201494323212201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6988 0.2358676049615905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4756 0.1605303848307562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3524 0.11894639952556452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.37532348256426E-5 2 0.0 0.0 0.0 0.0 3.37532348256426E-5 3 0.0 0.0 0.0 0.0 3.37532348256426E-5 4 0.0 0.0 0.0 0.0 3.37532348256426E-5 5 0.0 0.0 0.0 0.0 1.012597044769278E-4 6 0.0 0.0 0.0 0.0 1.012597044769278E-4 7 0.0 0.0 0.0 0.0 1.012597044769278E-4 8 0.0 0.0 0.0 0.0 1.012597044769278E-4 9 0.0 0.0 0.0 0.0 1.012597044769278E-4 10-11 0.0 0.0 0.0 3.37532348256426E-5 1.012597044769278E-4 12-13 0.0 0.0 0.0 6.75064696512852E-5 2.362726437794982E-4 14-15 0.0 0.0 0.0 6.75064696512852E-5 4.050388179077112E-4 16-17 0.0 0.0 0.0 1.012597044769278E-4 5.06298522384639E-4 18-19 0.0 0.0 0.0 1.350129393025704E-4 5.06298522384639E-4 20-21 0.0 0.0 0.0 1.68766174128213E-4 6.075582268615669E-4 22-23 0.0 0.0 0.0 3.206557308436047E-4 6.413114616872095E-4 24-25 0.0 0.0 0.0 5.906816094487456E-4 6.919413139256734E-4 26-27 0.0 0.0 0.0 0.0010463502795949207 7.594477835769586E-4 28-29 0.0 0.0 0.0 0.002919654812418085 7.763244009897799E-4 30-31 0.0 0.0 0.0 0.008522691793474757 7.763244009897799E-4 32-33 0.0 0.0 0.0 0.019070577676488072 8.43830870641065E-4 34-35 0.0 0.0 0.0 0.038124278735563316 9.113373402923503E-4 36-37 0.0 0.0 0.0 0.06954854035823659 9.113373402923503E-4 38-39 0.0 0.0 0.0 0.11823758159422604 9.788438099436355E-4 40-41 0.0 0.0 0.0 0.1717702120276952 9.788438099436355E-4 42-43 0.0 0.0 0.0 0.22666984847160287 9.957204273564567E-4 44-45 0.0 0.0 0.0 0.2959146097164087 0.0010463502795949207 46-47 0.0 0.0 0.0 0.379943287814846 0.0010969801318333845 48-49 0.0 0.0 0.0 0.4735578846037657 0.0012151164537231337 50-51 0.0 0.0 0.0 0.5756614199513346 0.001350129393025704 52-53 0.0 0.0 0.0 0.6786256627869574 0.0015526488019795598 54-55 0.0 0.0 0.0 0.7970995170249628 0.0015526488019795598 56-57 0.0 0.0 0.0 0.9361122146543719 0.0016032786542180236 58-59 0.0 0.0 0.0 1.0918833933747125 0.0016539085064564876 60-61 0.0 0.0 0.0 1.2652568840566256 0.0016539085064564876 62-63 0.0 0.0 0.0 1.4380396931290904 0.0018564279154103431 64-65 0.0 0.0 0.0 1.6206615701532296 0.0018564279154103431 66-67 0.0 0.0 0.0 1.8404963885726398 0.0019239343850616284 68-69 3.37532348256426E-5 0.0 0.0 2.0909791442137333 0.001957687619887271 70-71 3.37532348256426E-5 0.0 0.0 2.364701002032282 0.001957687619887271 72-73 3.37532348256426E-5 0.0 0.0 2.6526667249472524 0.002058947324364199 74-75 3.37532348256426E-5 0.0 0.0 2.945003491772143 0.0022108368810795902 76-77 3.37532348256426E-5 0.0 0.0 3.2667055728953422 0.002227713498492412 78-79 3.37532348256426E-5 0.0 0.0 3.634919611608278 0.0022614667333180545 80-81 3.37532348256426E-5 0.0 0.0 4.039823416576686 0.002295219968143697 82-83 3.37532348256426E-5 0.0 0.0 4.457249671665409 0.0023289732029693395 84-85 3.37532348256426E-5 0.0 0.0 4.887924071423194 0.002362726437794982 86-87 3.37532348256426E-5 0.0 0.0 5.342124475854455 0.002396479672620625 88-89 3.37532348256426E-5 0.0 0.0 5.840963533342627 0.0024302329074462675 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14730 0.0 28.703817 1 GTACATG 13710 0.0 28.589546 1 TACATGG 13690 0.0 28.05534 2 GGTATCA 10460 0.0 27.219757 1 ACATGGG 13690 0.0 26.704527 3 CATGGGG 6855 0.0 24.625015 4 ATCAACG 17390 0.0 23.994797 3 TCAACGC 17620 0.0 23.735403 4 TATCAAC 17720 0.0 23.708494 2 CAACGCA 17660 0.0 23.627544 5 AACGCAG 18125 0.0 23.032732 6 TAGTCCG 105 0.0022196968 22.579136 5 GTATCGC 130 2.991344E-4 21.870337 6 GTAAGGT 1280 0.0 21.856268 4 TAAGGTG 1430 0.0 21.552813 5 GAGTACT 9490 0.0 20.84602 12-13 CATGGGA 6560 0.0 20.817213 4 ACGCAGA 20225 0.0 20.547482 7 GTCCTAC 935 0.0 20.300795 1 CGCAGAG 20490 0.0 20.258957 8 >>END_MODULE