FastQCFastQC Report
Fri 27 May 2016
SRR939329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939329_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962679
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97760.32997162365548205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79130.2670893471753099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53610.18095109190027No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39120.13204265463791384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106050.028.7700651
GTATCAA147300.027.3376481
GTACATG132400.026.5315061
TACATGG131300.026.1674082
ACATGGG132250.025.0464763
CATGGGG62450.022.4929374
ATCAACG179100.022.2307073
TCAACGC181050.021.9912724
CAACGCA181500.021.9628945
TATCAAC182450.021.8225272
GTGTAGC17700.021.513461
AACGCAG186400.021.4364626
GAGTACT103200.020.30264312-13
ATACCGT2601.5086698E-820.0773436
GTACTTT107850.019.22927314-15
TACCGTA1751.00152225E-418.9822147
CATGGGA67600.018.9541364
AGTACTT108500.018.93911712-13
ACGCAGA211250.018.9148247
CGCAGAG213500.018.604358
Produced by FastQC (version 0.11.5)