##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2962679 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.296759453184094 33.0 31.0 34.0 31.0 34.0 2 32.49606926703838 34.0 31.0 34.0 31.0 34.0 3 32.589820226895995 34.0 31.0 34.0 31.0 34.0 4 36.11871181454352 37.0 35.0 37.0 35.0 37.0 5 36.05907119873601 37.0 35.0 37.0 35.0 37.0 6 36.09233399905963 37.0 35.0 37.0 35.0 37.0 7 36.03586112434051 37.0 35.0 37.0 35.0 37.0 8 36.00544743456851 37.0 35.0 37.0 35.0 37.0 9 37.710745241047036 39.0 37.0 39.0 35.0 39.0 10-11 37.633003777999576 39.0 37.0 39.0 35.0 39.0 12-13 37.55560626041498 39.0 37.0 39.0 35.0 39.0 14-15 38.84941854990028 40.0 38.0 41.0 35.0 41.0 16-17 38.84883276250987 40.0 38.0 41.0 35.0 41.0 18-19 38.95128868162902 40.0 38.0 41.0 35.0 41.0 20-21 38.93094324427317 40.0 38.0 41.0 35.0 41.0 22-23 38.85390317344539 40.0 38.0 41.0 35.0 41.0 24-25 38.7870164131855 40.0 38.0 41.0 34.0 41.0 26-27 38.627300325144915 40.0 38.0 41.0 34.0 41.0 28-29 38.460093212933295 40.0 38.0 41.0 34.0 41.0 30-31 38.26244085167512 40.0 38.0 41.0 34.0 41.0 32-33 38.20549644426548 40.0 38.0 41.0 34.0 41.0 34-35 38.10856204806528 40.0 38.0 41.0 33.0 41.0 36-37 37.95250430438127 40.0 37.5 41.0 33.0 41.0 38-39 37.8170464974437 40.0 37.0 41.0 33.0 41.0 40-41 37.62991231922189 40.0 37.0 41.0 33.0 41.0 42-43 37.43744850522111 40.0 37.0 41.0 32.0 41.0 44-45 37.19008589860731 40.0 36.5 41.0 31.5 41.0 46-47 36.87698616691178 40.0 36.0 41.0 31.0 41.0 48-49 36.55496916810765 39.5 36.0 41.0 30.0 41.0 50-51 36.300228441893296 39.0 35.5 41.0 30.0 41.0 52-53 36.03123439967678 39.0 35.0 40.5 29.0 41.0 54-55 35.77478896633757 39.0 35.0 40.0 28.5 41.0 56-57 35.45531274228494 38.0 35.0 40.0 28.0 41.0 58-59 35.12566548721613 38.0 34.0 40.0 27.5 41.0 60-61 34.77935139108894 38.0 34.0 40.0 27.0 41.0 62-63 34.427445227782016 37.0 34.0 40.0 26.0 41.0 64-65 34.11702212760815 37.0 33.5 39.5 26.0 41.0 66-67 33.69875372931054 36.0 33.0 39.0 25.5 40.5 68-69 33.23576313869981 35.5 33.0 39.0 24.5 40.0 70-71 32.753882381452726 35.0 32.0 38.0 23.5 40.0 72-73 32.26334155674644 35.0 31.5 37.5 22.0 39.5 74-75 31.744142041712923 35.0 31.0 37.0 20.5 39.0 76-77 29.805092283031676 33.0 28.5 35.0 18.0 37.0 78-79 30.77160704889055 34.0 30.5 36.0 18.5 38.0 80-81 30.793465137465112 34.0 31.0 35.5 18.5 37.0 82-83 30.439925655124974 34.0 30.5 35.0 17.5 37.0 84-85 29.996596492566358 34.0 30.0 35.0 10.5 36.0 86-87 29.399340596804446 34.0 29.5 35.0 6.0 36.0 88-89 28.800719551460013 34.0 29.0 35.0 2.0 35.5 90-91 28.30829343982254 33.0 28.5 35.0 2.0 35.0 92-93 27.94828734398833 33.0 27.0 35.0 2.0 35.0 94-95 27.4224348976045 33.0 27.0 35.0 2.0 35.0 96-97 26.676186316506108 33.0 25.5 34.0 2.0 35.0 98-99 25.652064736004135 32.0 24.0 34.0 2.0 35.0 100-101 21.330470665232376 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2665146049212339 1101 2 0.25577695782116905 1101 3 0.2516752314243291 1101 4 0.15025060149202574 1101 5 0.19202676698638754 1101 6 0.21241778276839796 1101 7 0.2251735206216381 1101 8 0.20351145904027845 1101 9 0.27001237893153274 1101 10-11 0.31883584882198335 1101 12-13 0.2811905089619131 1101 14-15 0.3780335547249578 1101 16-17 0.23919945520619734 1101 18-19 0.2900477188876778 1101 20-21 0.40189327244522843 1101 22-23 0.4172122605191575 1101 24-25 0.4166637629081862 1101 26-27 0.4013620939043747 1101 28-29 0.38743545939805557 1101 30-31 0.44000486091358226 1101 32-33 0.5449430923772312 1101 34-35 0.5752364521712536 1101 36-37 0.5362517814710586 1101 38-39 0.4958549954650593 1101 40-41 0.6078827800314599 1101 42-43 0.6256365095860588 1101 44-45 0.7014103503034903 1101 46-47 0.7212857356202775 1101 48-49 0.7341572280814219 1101 50-51 0.7736207666720887 1101 52-53 0.6949417189401075 1101 54-55 0.606018331066636 1101 56-57 0.5818794684535504 1101 58-59 0.5849684688431154 1101 60-61 0.5940954477499574 1101 62-63 0.5837925080021407 1101 64-65 0.6478613370703812 1101 66-67 0.47496779634136743 1101 68-69 0.5199467567997473 1101 70-71 0.4259941318681584 1101 72-73 0.5291298867855545 1101 74-75 0.5976385590555786 1101 76-77 0.5980768919686135 1101 78-79 0.6557499850603037 1101 80-81 0.6229578992936595 1101 82-83 0.6658102005410065 1101 84-85 0.6474422996641316 1101 86-87 0.47116762456150596 1101 88-89 0.763096923264655 1101 90-91 0.9675149652325992 1101 92-93 1.0198728134179227 1101 94-95 0.8477745509827948 1101 96-97 0.8374378865643273 1101 98-99 0.7982700162687806 1101 100-101 0.7532161964871591 1102 1 0.20815521641990387 1102 2 0.22917248853276107 1102 3 0.17775086386065908 1102 4 0.12536774156287578 1102 5 0.15102823076614413 1102 6 0.16179788893221314 1102 7 0.1744782461761929 1102 8 0.2166613271381692 1102 9 0.2722729596469975 1102 10-11 0.2872076807075814 1102 12-13 0.3018819976256495 1102 14-15 0.316637863201386 1102 16-17 0.37218864813952024 1102 18-19 0.3232804470263986 1102 20-21 0.335447638476154 1102 22-23 0.3588882590260667 1102 24-25 0.35783387377072984 1102 26-27 0.27658471099070425 1102 28-29 0.24269024166954267 1102 30-31 0.2869630997768695 1102 32-33 0.3669422531397757 1102 34-35 0.3148358824055322 1102 36-37 0.4092019523756534 1102 38-39 0.378381086229453 1102 40-41 0.43474043045137023 1102 42-43 0.4480604540411548 1102 44-45 0.5044382358243524 1102 46-47 0.5185088256501231 1102 48-49 0.5210864677451283 1102 50-51 0.5335928535116707 1102 52-53 0.4278864373139655 1102 54-55 0.43258269507904146 1102 56-57 0.4979274557494051 1102 58-59 0.5843644468129909 1102 60-61 0.563129023669255 1102 62-63 0.5741955792873554 1102 64-65 0.5411272103421751 1102 66-67 0.49501903840441486 1102 68-69 0.5990447468456068 1102 70-71 0.32601585265112476 1102 72-73 0.4147886700364296 1102 74-75 0.34333746712557556 1102 76-77 0.41362410075028677 1102 78-79 0.49807769081237296 1102 80-81 0.7792096180413495 1102 82-83 0.7470702879322637 1102 84-85 0.46569190328776244 1102 86-87 0.4123071643991807 1102 88-89 0.5722057904532356 1102 90-91 0.6719531102777303 1102 92-93 0.5866818796392259 1102 94-95 0.5576722762472777 1102 96-97 0.36265207218991335 1102 98-99 0.1767446086760529 1102 100-101 0.006632962195570968 1103 1 0.16917320380350276 1103 2 0.22969124112825057 1103 3 0.2050454012231384 1103 4 0.1324470912308584 1103 5 0.11531028408148103 1103 6 0.12073050469692959 1103 7 0.14313854376749902 1103 8 0.20812491125856525 1103 9 0.16611476538678716 1103 10-11 0.19837513238761062 1103 12-13 0.21682308086948154 1103 14-15 0.2916145089733604 1103 16-17 0.1780862780134811 1103 18-19 0.2059545662314619 1103 20-21 0.2697482784690379 1103 22-23 0.2838051411834357 1103 24-25 0.26717809312613383 1103 26-27 0.26211807569325174 1103 28-29 0.19296659658086668 1103 30-31 0.2524941656663273 1103 32-33 0.2979744064065102 1103 34-35 0.3103093342916736 1103 36-37 0.34064593027278534 1103 38-39 0.39943878063596117 1103 40-41 0.4604881732904431 1103 42-43 0.45969446531252345 1103 44-45 0.4107340411259415 1103 46-47 0.40475916031808623 1103 48-49 0.39769871074846463 1103 50-51 0.4185317318842863 1103 52-53 0.20960467024506357 1103 54-55 0.2755767305984591 1103 56-57 0.3926038936611391 1103 58-59 0.41605264597199465 1103 60-61 0.5116069441496691 1103 62-63 0.4567545506126933 1103 64-65 0.45487260684461006 1103 66-67 0.2602447379191446 1103 68-69 0.29499480181541315 1103 70-71 0.4348654818155424 1103 72-73 0.4665850460349432 1103 74-75 0.36446762111566144 1103 76-77 0.4598315564753399 1103 78-79 0.5015748845749641 1103 80-81 0.4860994814084094 1103 82-83 0.5058836942123897 1103 84-85 0.4159695015475151 1103 86-87 0.4422426429045885 1103 88-89 0.4062000555776706 1103 90-91 0.5324065207686637 1103 92-93 0.32304027510084055 1103 94-95 0.21316335881417814 1103 96-97 0.20948542951526505 1103 98-99 -0.052018102144035794 1103 100-101 -0.36003242428271065 1104 1 0.12355067598106473 1104 2 0.1617264751244818 1104 3 0.08916923482131978 1104 4 0.05467904589643524 1104 5 0.1593602081391552 1104 6 0.10587484913808254 1104 7 0.1351781404796597 1104 8 0.1896091666489852 1104 9 0.10054192930402195 1104 10-11 0.08623097906853872 1104 12-13 0.2161136330231912 1104 14-15 0.21500879651685523 1104 16-17 0.23284964119472562 1104 18-19 0.12870207776125397 1104 20-21 0.20941320027444732 1104 22-23 0.1842081858999265 1104 24-25 0.14762704766135926 1104 26-27 0.22353133902502975 1104 28-29 0.23118155691459208 1104 30-31 0.2457880769762255 1104 32-33 0.17269868073355354 1104 34-35 0.30199498372516587 1104 36-37 0.37409087529648843 1104 38-39 0.10316856508673311 1104 40-41 0.30038939761981 1104 42-43 0.26369492724715826 1104 44-45 0.21796554668839718 1104 46-47 0.22122179460159686 1104 48-49 0.25460095281977146 1104 50-51 0.37564927331151665 1104 52-53 0.2978247969529306 1104 54-55 0.3323397844726088 1104 56-57 0.30501001253907845 1104 58-59 0.2405405840079169 1104 60-61 0.40448200771632514 1104 62-63 0.3180581382374399 1104 64-65 0.3166580517166935 1104 66-67 0.4126238700898526 1104 68-69 0.3735519179386202 1104 70-71 0.2854199046707748 1104 72-73 0.352487192882883 1104 74-75 0.3395444245010246 1104 76-77 0.39809142712013923 1104 78-79 0.5880337166772591 1104 80-81 0.5591730823171517 1104 82-83 0.5232719536440591 1104 84-85 0.402398888693984 1104 86-87 0.4252444616033486 1104 88-89 0.5447277672656305 1104 90-91 0.5016366769957692 1104 92-93 0.3574774340948217 1104 94-95 0.6120213537612607 1104 96-97 0.5795824560474685 1104 98-99 0.599930026109007 1104 100-101 0.42227358849883245 1105 1 0.06805340472661214 1105 2 0.01914744938199675 1105 3 0.023588929307130968 1105 4 0.006884035400766209 1105 5 0.008314397705902365 1105 6 0.028650740114116502 1105 7 0.038516655828892965 1105 8 -0.005153208704911094 1105 9 0.02852834690001771 1105 10-11 0.07418430111316354 1105 12-13 0.1282380091103974 1105 14-15 0.06836789223903139 1105 16-17 0.044397831052997105 1105 18-19 0.05950996495641192 1105 20-21 0.08057293800150944 1105 22-23 0.10239019088415802 1105 24-25 0.0998579151385286 1105 26-27 0.12823201178811416 1105 28-29 0.09219019424988772 1105 30-31 0.15407275265715725 1105 32-33 -0.0011634750749678346 1105 34-35 0.13479168624213145 1105 36-37 0.03307993912390117 1105 38-39 0.20555216397429632 1105 40-41 0.25769549422619775 1105 42-43 0.20915225138770666 1105 44-45 0.14515302200640434 1105 46-47 0.08973510827281217 1105 48-49 0.22897309091182905 1105 50-51 0.2006119493122327 1105 52-53 0.15477861921941383 1105 54-55 0.16308454996230637 1105 56-57 0.2024576610147406 1105 58-59 0.17031065532966494 1105 60-61 0.015845263178157154 1105 62-63 0.17674983012388878 1105 64-65 0.1092161118062549 1105 66-67 0.06575391685642984 1105 68-69 0.1997255931694255 1105 70-71 0.03912671558627068 1105 72-73 -0.018588168525802473 1105 74-75 0.04998756712717878 1105 76-77 0.2834015357755213 1105 78-79 0.2505473218770007 1105 80-81 0.29810562556964726 1105 82-83 0.26274510893950875 1105 84-85 0.35482509312739907 1105 86-87 0.38289942924303944 1105 88-89 0.43477977364329945 1105 90-91 0.5238790836087119 1105 92-93 0.43576530117836043 1105 94-95 0.5889565027907722 1105 96-97 0.5347670725879219 1105 98-99 0.35679491291053367 1105 100-101 0.14156038386185799 1106 1 0.008361226093718699 1106 2 0.010639060886767027 1106 3 0.07223076230278025 1106 4 0.04723526766151309 1106 5 -0.029416048509688153 1106 6 0.062085927723245504 1106 7 -0.02611131207930839 1106 8 0.010627971059449237 1106 9 0.008444208961904565 1106 10-11 0.07662121415865641 1106 12-13 0.009328091847748965 1106 14-15 0.12027819154688046 1106 16-17 0.06699661100156362 1106 18-19 0.11687155790154691 1106 20-21 0.14418602961242755 1106 22-23 0.14507475867812047 1106 24-25 0.12426077703102578 1106 26-27 0.1592027878971649 1106 28-29 0.20594616783083808 1106 30-31 0.15420566185432705 1106 32-33 0.12062003069201666 1106 34-35 0.21985503901504444 1106 36-37 0.21144958141666592 1106 38-39 0.3554180081805214 1106 40-41 0.21968621368110064 1106 42-43 0.17111198925044135 1106 44-45 0.18955715982718857 1106 46-47 0.10325958153075021 1106 48-49 0.14657800484899752 1106 50-51 0.23762596912431633 1106 52-53 0.3646101600201561 1106 54-55 0.2573435692720807 1106 56-57 0.33832330869868343 1106 58-59 0.3718477456118592 1106 60-61 0.3904992409579293 1106 62-63 0.4123677717289098 1106 64-65 0.3214435012936221 1106 66-67 0.4657249884822008 1106 68-69 0.43963604353545094 1106 70-71 0.4504669315994647 1106 72-73 0.45786618807269264 1106 74-75 0.47395855662423614 1106 76-77 0.5037288700460003 1106 78-79 0.5833814665980626 1106 80-81 0.5619395481032008 1106 82-83 0.6196064866634146 1106 84-85 0.5334972184206244 1106 86-87 0.550089666485043 1106 88-89 0.7901577014037571 1106 90-91 0.8081425246699929 1106 92-93 0.9425414703576536 1106 94-95 1.130518400345462 1106 96-97 0.9936708155309013 1106 98-99 1.1808026036783126 1106 100-101 1.113229826039209 1107 1 0.10097257096372658 1107 2 0.09092860455574225 1107 3 0.09629293778439063 1107 4 0.027765659149260102 1107 5 0.080986529528424 1107 6 0.042754469405011264 1107 7 0.024321522645450955 1107 8 0.11740407660189334 1107 9 0.10086130628638301 1107 10-11 0.08899438809417859 1107 12-13 0.07798120281069743 1107 14-15 0.11623074969374869 1107 16-17 0.18498746222923756 1107 18-19 0.1631111455034926 1107 20-21 0.16799116200576947 1107 22-23 0.22250118442998712 1107 24-25 0.21343642824472653 1107 26-27 0.1843174764803166 1107 28-29 0.17077707558167532 1107 30-31 0.21166137066290247 1107 32-33 0.1798056669950583 1107 34-35 0.310991585709921 1107 36-37 0.28243162954651524 1107 38-39 0.1884506405720643 1107 40-41 0.32572450149902465 1107 42-43 0.28396072280189344 1107 44-45 0.30981804002214375 1107 46-47 0.24117952522766473 1107 48-49 0.2778340257454417 1107 50-51 0.39785364064406536 1107 52-53 0.346666662612229 1107 54-55 0.4486711220663864 1107 56-57 0.3766314006160414 1107 58-59 0.48073922367061783 1107 60-61 0.4165409711677057 1107 62-63 0.32114576329062317 1107 64-65 0.22167519890474097 1107 66-67 0.4188031422597547 1107 68-69 0.3596273454556993 1107 70-71 0.35663292760973064 1107 72-73 0.42563281734909 1107 74-75 0.28913100036122685 1107 76-77 0.24054204529245027 1107 78-79 0.4277757264395632 1107 80-81 0.5015806518407189 1107 82-83 0.5350725623582342 1107 84-85 0.5080561724310861 1107 86-87 0.7119967898439548 1107 88-89 0.8037888793787644 1107 90-91 0.8940499968132904 1107 92-93 0.7155474846157084 1107 94-95 0.8830925989607046 1107 96-97 0.7506569691735372 1107 98-99 0.8720449339503347 1107 100-101 0.946314907660259 1108 1 0.06229095597529977 1108 2 0.05730841717770119 1108 3 0.0389749859431987 1108 4 0.03265372760291996 1108 5 0.11460250805555461 1108 6 -0.02043096187367155 1108 7 0.05712542045090885 1108 8 0.038466963887273664 1108 9 0.1280293252075566 1108 10-11 0.00598253892756162 1108 12-13 0.030372444396697063 1108 14-15 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-0.7659496748151788 2316 78-79 -0.7532449821817195 2316 80-81 -0.8245992180043515 2316 82-83 -0.7951190190597153 2316 84-85 -0.6781008906667942 2316 86-87 -0.8421203495925127 2316 88-89 -1.191635594015338 2316 90-91 -1.1533153497331021 2316 92-93 -1.1449261465645755 2316 94-95 -0.782218575634186 2316 96-97 -0.5571775515173059 2316 98-99 -0.4144937272793783 2316 100-101 0.06303334390449322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 32.0 5 70.0 6 151.0 7 358.0 8 852.0 9 1580.0 10 2745.0 11 4180.0 12 6165.0 13 8411.0 14 10716.0 15 14226.0 16 18141.0 17 24946.0 18 24215.0 19 17152.0 20 16259.0 21 17171.0 22 18352.0 23 20020.0 24 23545.0 25 27673.0 26 33160.0 27 40113.0 28 48687.0 29 59431.0 30 72294.0 31 88942.0 32 110039.0 33 141524.0 34 194526.0 35 281445.0 36 441646.0 37 679104.0 38 484601.0 39 30194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.219512492353815 19.150011492552522 14.31041130474492 23.32006471034875 2 15.731808947239982 20.6151594553443 39.535805262736865 24.117226334678847 3 19.71911233042797 24.2631415688301 29.545624078747647 26.47212202199428 4 13.127105568979966 16.319824051137502 36.423723258577795 34.12934712130474 5 13.642213685654097 37.747491375204675 33.86897466785973 14.741320271281499 6 32.659056212299745 35.76151854453351 16.99785903231501 14.581566210851731 7 29.08121332078163 32.75761565799063 20.103730441266165 18.057440579961582 8 26.669645952193942 35.23868093708431 19.392077238202315 18.699595872519435 9 26.679096857945122 16.808942176995888 19.98377144469583 36.528189520363156 10-11 25.389503891579206 26.56070738679418 26.813080323585513 21.236708398041095 12-13 26.088955300253584 23.720355799598945 27.638920720064508 22.55176818008296 14-15 22.64889447549123 25.274151277398037 26.00598080734317 26.07097343976756 16-17 22.13846485149484 28.570341227370825 26.820898665995195 22.470295255139135 18-19 22.125014682672017 27.006294309603884 29.204678473110118 21.664012534613978 20-21 22.822911767172847 26.154305976838188 29.187591566212806 21.83519068977616 22-23 22.4477069571155 26.068163307600994 29.228090522125417 22.25603921315809 24-25 22.27681432919327 26.329109566038035 29.280914334627546 22.113161770141147 26-27 21.997680475354038 26.56161186710933 29.219107995609374 22.221599661927257 28-29 21.8861712659387 26.889430140761117 29.17320776229892 22.051190831001264 30-31 22.170120353909418 26.42731460276324 29.097533009819827 22.30503203350751 32-33 21.942370401923394 26.597802191867565 28.969506990126163 22.490320416082877 34-35 22.46005288196945 26.675809427113123 28.818496234180678 22.045641456736746 36-37 22.60240031703574 26.204023572444623 28.997225937250416 22.196350173269217 38-39 22.184400624723004 26.610776438072996 28.95338644221464 22.251436494989356 40-41 22.46716421663727 26.51631591143338 28.917244020295126 22.09927585163422 42-43 22.207228242222683 26.527992228632918 28.683378728751645 22.581400800392753 44-45 22.28011541734997 26.758807475563597 28.622652199162268 22.338424907924168 46-47 22.606178977812107 26.979592256928665 28.061098173289032 22.353130591970192 48-49 22.48579488879273 26.9954999989874 27.957589549412592 22.56111556280728 50-51 22.496309925111646 27.229866772516882 27.951663309905193 22.322159992466272 52-53 22.767251166301822 27.126012576803443 27.737334805200636 22.369401451694102 54-55 22.267920351816716 27.1113407831223 27.918009342220333 22.702729522840645 56-57 22.4075516099928 27.37895730617119 27.650538216929217 22.562952866906787 58-59 22.48236567832331 26.930256774625818 27.819470451382937 22.767907095667937 60-61 22.29386025683369 27.123917335674925 28.190595163496003 22.39162724399538 62-63 22.220401398752465 27.090095320389924 28.23807482515594 22.451428455701674 64-65 22.00262532926314 27.09320897728945 28.088424113875426 22.815741579571984 66-67 21.974399426462902 27.276697895275476 27.81405138881576 22.934851289445866 68-69 22.16742300389164 27.035878720739504 27.981945792484964 22.814752482883893 70-71 22.22204970568867 27.044627514489424 27.77457497082877 22.958747808993145 72-73 22.188262717628202 27.07890392445486 27.747049207828457 22.985784150088485 74-75 22.05787769412037 27.468184617399423 27.523691821437936 22.950245867042273 76-77 22.078015247594912 27.06605452492294 27.743851369620337 23.11207885786181 78-79 22.205075549993015 27.176294118083867 27.65461201603686 22.964018315886257 80-81 22.272674034562705 27.257193082057995 27.494799459812135 22.97533342356716 82-83 22.222758523620005 27.23568770021997 27.580983292486295 22.960570483673727 84-85 22.15450948280256 27.25879178945812 27.424452665982376 23.162246061756946 86-87 22.16334259383779 27.094290902382205 27.451020806738384 23.291345697041617 88-89 22.06130141899896 27.102424421776856 27.315149760637407 23.521124398586775 90-91 22.034403418206335 27.062390930272546 27.472100488334533 23.43110516318659 92-93 22.184532985179967 26.8767726777015 27.43601314891016 23.502681188208374 94-95 21.97578610440078 26.76504947042862 27.56997973793314 23.689184687237464 96-97 21.958166915821796 26.47222328169876 27.713194713298332 23.85641508918111 98-99 21.87696929295316 26.692566683824587 27.35016496915794 24.080299054064316 100-101 21.896689705592966 26.084706786149102 28.037609356704618 23.980994151553315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 600.0 1 424.5 2 355.5 3 506.0 4 456.5 5 421.0 6 805.5 7 1639.5 8 2541.5 9 2966.0 10 2750.0 11 2512.5 12 2620.0 13 2800.0 14 3035.0 15 3311.0 16 3534.5 17 3793.5 18 4034.5 19 4382.5 20 4982.0 21 5806.5 22 6772.0 23 8112.5 24 10054.5 25 12583.0 26 16184.5 27 20676.5 28 24627.5 29 28701.5 30 33671.5 31 40103.0 32 48009.0 33 52887.0 34 55662.0 35 59860.0 36 66257.0 37 72780.0 38 77045.5 39 80326.5 40 83856.5 41 89680.0 42 93950.5 43 97536.0 44 102479.0 45 107544.5 46 109412.5 47 112662.0 48 121175.5 49 119738.0 50 111907.0 51 107348.5 52 104485.5 53 104191.5 54 103133.5 55 95823.5 56 85526.0 57 77092.5 58 70919.5 59 62727.0 60 51223.0 61 42520.5 62 35847.5 63 26994.0 64 19086.0 65 14454.5 66 10882.0 67 8725.5 68 6721.0 69 4574.5 70 3244.0 71 2265.0 72 1705.5 73 1415.0 74 1147.0 75 869.0 76 575.5 77 337.5 78 204.0 79 143.5 80 99.5 81 62.5 82 37.0 83 28.0 84 14.0 85 6.5 86 4.5 87 3.5 88 3.5 89 2.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29000779362192125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011982398363103125 16-17 7.088179313384946E-4 18-19 1.012597044769278E-4 20-21 1.68766174128213E-5 22-23 0.0 24-25 0.0 26-27 1.012597044769278E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001468265714915453 36-37 0.007999516653677298 38-39 0.002902778195005264 40-41 0.0032234339258488684 42-43 2.869024960179621E-4 44-45 0.0013332527756128828 46-47 1.5188955671539172E-4 48-49 4.3879205273335384E-4 50-51 6.75064696512852E-5 52-53 8.43830870641065E-5 54-55 0.0 56-57 8.775841054667077E-4 58-59 0.0020758239417770197 60-61 7.594477835769585E-4 62-63 0.0013163761582000615 64-65 1.012597044769278E-4 66-67 3.7128558308206864E-4 68-69 0.0015357721845667385 70-71 0.0 72-73 0.0 74-75 1.68766174128213E-5 76-77 0.25618705232662736 78-79 1.68766174128213E-4 80-81 2.025194089538556E-4 82-83 0.0 84-85 0.0 86-87 0.1065589623445537 88-89 0.3707961611770968 90-91 0.2749876041245103 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.350129393025704E-4 100-101 4.050388179077112E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2962679.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.35564150383924 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48730077744541 33.853269876938334 2 14.842445613977947 14.057470670715416 3 5.436779111655532 7.72386487641363 4 2.641616862853837 5.00381844591211 5 1.3902764330834807 3.2918716178168825 6 0.8694293044562555 2.470342947285764 7 0.5977139038948218 1.9813587748292383 8 0.42952017507981116 1.627216274379664 9 0.3200002956952977 1.3638437355662163 >10 1.777764920370755 15.354441075691058 >50 0.1363664318131376 4.428133464112265 >100 0.06472468682023101 5.384356650461639 >500 0.004421316904996658 1.5299182598854093 >1k 0.0014262312595954357 1.1516937209305096 >5k 2.1393468893931533E-4 0.7783996090619354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9776 0.32997162365548205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7913 0.2670893471753099 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5361 0.18095109190027 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3912 0.13204265463791384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.012597044769278E-4 2 0.0 0.0 0.0 0.0 1.012597044769278E-4 3 0.0 0.0 0.0 0.0 1.012597044769278E-4 4 0.0 0.0 0.0 0.0 1.012597044769278E-4 5 0.0 0.0 0.0 0.0 1.012597044769278E-4 6 0.0 0.0 0.0 0.0 1.350129393025704E-4 7 0.0 0.0 0.0 0.0 1.350129393025704E-4 8 0.0 0.0 0.0 0.0 1.68766174128213E-4 9 0.0 0.0 0.0 0.0 1.68766174128213E-4 10-11 0.0 0.0 0.0 0.0 2.025194089538556E-4 12-13 0.0 0.0 0.0 3.37532348256426E-5 4.556686701461751E-4 14-15 0.0 0.0 0.0 3.37532348256426E-5 5.569283746231029E-4 16-17 0.0 0.0 0.0 6.75064696512852E-5 6.075582268615669E-4 18-19 0.0 0.0 0.0 1.012597044769278E-4 6.075582268615669E-4 20-21 0.0 0.0 0.0 1.350129393025704E-4 7.256945487513159E-4 22-23 0.0 0.0 0.0 2.869024960179621E-4 7.594477835769586E-4 24-25 0.0 0.0 0.0 5.569283746231029E-4 7.763244009897799E-4 26-27 0.0 0.0 0.0 0.0010294736621820995 8.100776358154224E-4 28-29 0.0 0.0 0.0 0.0029027781950052636 8.775841054667077E-4 30-31 0.0 0.0 0.0 0.008472061941236293 8.944607228795289E-4 32-33 0.0 0.0 0.0 0.01915496076355218 9.113373402923503E-4 34-35 0.0 0.0 0.0 0.03820866182262743 9.450905751179928E-4 36-37 0.0 0.0 0.0 0.07012234535027251 9.450905751179928E-4 38-39 0.0 0.0 0.0 0.11963834083949021 9.957204273564567E-4 40-41 0.0 0.0 0.0 0.17376165288240814 0.001012597044769278 42-43 0.0 0.0 0.0 0.22908320476163635 0.001012597044769278 44-45 0.0 0.0 0.0 0.2985811152676345 0.0010463502795949207 46-47 0.0 0.0 0.0 0.3830992152710435 0.0010969801318333845 48-49 0.0 0.0 0.0 0.4776251494002557 0.0011982398363103125 50-51 0.0 0.0 0.0 0.5804206260617502 0.0013163761582000615 52-53 0.0 0.0 0.0 0.6840768102112986 0.001400759245264168 54-55 0.0 0.0 0.0 0.8034282485547709 0.0014513890975026318 56-57 0.0 0.0 0.0 0.9428797382369133 0.0014513890975026318 58-59 0.0 0.0 0.0 1.0995285010627205 0.0014513890975026318 60-61 0.0 0.0 0.0 1.2740496017287057 0.0014513890975026318 62-63 0.0 0.0 0.0 1.4475581053499216 0.0015526488019795598 64-65 0.0 0.0 0.0 1.6321207933765352 0.0015864020368052023 66-67 1.68766174128213E-5 0.0 0.0 1.8527319361969352 0.0016032786542180236 68-69 3.37532348256426E-5 0.0 0.0 2.1032653216902677 0.0016201552716308448 70-71 3.37532348256426E-5 0.0 0.0 2.3773584650918984 0.0016539085064564874 72-73 3.37532348256426E-5 0.0 0.0 2.6657123502073627 0.001738291593520594 74-75 3.37532348256426E-5 0.0 0.0 2.9592811101033893 0.0018057980631718791 76-77 3.37532348256426E-5 0.0 0.0 3.2824008270892664 0.001957687619887271 78-79 3.37532348256426E-5 0.0 0.0 3.6519312419604013 0.0020083174721257347 80-81 3.37532348256426E-5 0.0 0.0 4.058455202200441 0.00207582394177702 82-83 3.37532348256426E-5 0.0 0.0 4.478210430492132 0.0023458498203821608 84-85 3.37532348256426E-5 0.0 0.0 4.909981810381753 0.002362726437794982 86-87 3.37532348256426E-5 0.0 0.0 5.365515467588625 0.0024133562900334462 88-89 3.37532348256426E-5 0.0 0.0 5.86634596593151 0.00246398614227191 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10605 0.0 28.770065 1 GTATCAA 14730 0.0 27.337648 1 GTACATG 13240 0.0 26.531506 1 TACATGG 13130 0.0 26.167408 2 ACATGGG 13225 0.0 25.046476 3 CATGGGG 6245 0.0 22.492937 4 ATCAACG 17910 0.0 22.230707 3 TCAACGC 18105 0.0 21.991272 4 CAACGCA 18150 0.0 21.962894 5 TATCAAC 18245 0.0 21.822527 2 GTGTAGC 1770 0.0 21.51346 1 AACGCAG 18640 0.0 21.436462 6 GAGTACT 10320 0.0 20.302643 12-13 ATACCGT 260 1.5086698E-8 20.077343 6 GTACTTT 10785 0.0 19.229273 14-15 TACCGTA 175 1.00152225E-4 18.982214 7 CATGGGA 6760 0.0 18.954136 4 AGTACTT 10850 0.0 18.939117 12-13 ACGCAGA 21125 0.0 18.914824 7 CGCAGAG 21350 0.0 18.60435 8 >>END_MODULE