FastQCFastQC Report
Fri 27 May 2016
SRR939328_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939328_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015491
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94250.31255274845787967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70380.23339482691210156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.1601065962392194No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34870.1156362264055837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148800.031.44561
GGTATCA99300.027.8419931
GTACATG137350.027.219031
TACATGG135550.026.6985512
ATCAACG174000.026.5777383
TATCAAC175950.026.2562312
TCAACGC176800.026.2373094
CAACGCA176900.026.1416055
AACGCAG181150.025.5632576
ACATGGG138400.025.2919883
CATGGGG66500.023.109264
ACGCAGA201100.022.7919547
CGCAGAG203750.022.4955168
GAGTACT95150.022.21887412-13
GCAGAGT215950.020.8731169
TACCTGG21400.020.6125562
GTACTTT102650.020.38784414-15
AGTACTT101900.020.16558612-13
AGAGTAC166850.019.84316410-11
GTACCGT2201.3742447E-619.3900876
Produced by FastQC (version 0.11.5)