##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939328_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3015491 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859690843050103 33.0 31.0 34.0 30.0 34.0 2 31.92588669639538 33.0 31.0 34.0 30.0 34.0 3 31.984619751808246 34.0 31.0 34.0 30.0 34.0 4 35.48491870809762 37.0 35.0 37.0 33.0 37.0 5 35.53355423710434 37.0 35.0 37.0 33.0 37.0 6 35.623503104469556 37.0 35.0 37.0 33.0 37.0 7 35.542815415466336 37.0 35.0 37.0 33.0 37.0 8 35.5225958227035 37.0 35.0 37.0 33.0 37.0 9 37.15086299378775 39.0 37.0 39.0 33.0 39.0 10-11 37.06196586227583 39.0 37.0 39.0 33.0 39.0 12-13 36.95909206825688 39.0 37.0 39.0 33.0 39.0 14-15 38.193736277110425 40.0 38.0 41.0 33.0 41.0 16-17 38.13013900555498 40.0 38.0 41.0 33.0 41.0 18-19 38.072922618571894 40.0 38.0 41.0 33.0 41.0 20-21 37.969437647136075 40.0 38.0 41.0 33.0 41.0 22-23 37.89641322093152 40.0 38.0 41.0 32.5 41.0 24-25 37.91254160599385 40.0 38.0 41.0 33.0 41.0 26-27 37.83096517283587 40.0 38.0 41.0 32.5 41.0 28-29 37.69892017585196 40.0 38.0 41.0 32.0 41.0 30-31 37.521959773715125 40.0 37.0 41.0 32.0 41.0 32-33 37.32169686462338 40.0 37.0 41.0 31.5 41.0 34-35 37.01846084103717 40.0 36.5 41.0 30.5 41.0 36-37 36.775613656283504 40.0 36.0 41.0 30.0 41.0 38-39 36.45308044361599 39.5 35.5 41.0 30.0 41.0 40-41 36.32253934765516 39.0 35.0 41.0 30.0 41.0 42-43 36.149686236835066 39.0 35.0 41.0 29.5 41.0 44-45 36.124377423112854 39.0 35.0 41.0 29.0 41.0 46-47 35.9413878204246 39.0 35.0 41.0 28.0 41.0 48-49 35.738130539935284 39.0 35.0 41.0 27.0 41.0 50-51 34.58017981151328 38.0 34.0 40.0 25.5 40.5 52-53 34.68547178552349 38.0 34.5 39.5 25.5 40.5 54-55 35.18433946577854 39.0 35.0 40.5 26.0 41.0 56-57 35.07799260551599 39.0 35.0 41.0 26.0 41.0 58-59 34.867074217764205 38.0 34.5 40.0 26.0 41.0 60-61 34.57602775136785 38.0 34.0 40.0 25.5 41.0 62-63 34.22030442140268 37.5 34.0 40.0 24.5 41.0 64-65 33.844913813372344 37.0 34.0 40.0 23.0 41.0 66-67 33.48767978415455 36.5 33.0 39.5 22.5 41.0 68-69 33.07809093112863 36.0 33.0 39.0 22.0 41.0 70-71 32.66903847499462 35.5 32.5 39.0 20.5 40.5 72-73 32.24612542368722 35.0 32.0 38.0 19.5 40.0 74-75 31.808538311008057 35.0 32.0 37.0 18.0 39.5 76-77 31.368133083468 35.0 31.0 37.0 15.5 39.0 78-79 30.9520534135237 35.0 31.0 36.0 11.5 39.0 80-81 30.505132497493776 35.0 31.0 36.0 7.5 37.5 82-83 30.07114579350427 34.0 30.0 35.0 4.5 37.0 84-85 29.668917267536198 34.0 30.0 35.0 2.0 36.5 86-87 29.352722160338068 34.0 30.0 35.0 2.0 36.0 88-89 28.95128173156544 34.0 29.0 35.0 2.0 36.0 90-91 28.560824091333714 34.0 29.0 35.0 2.0 35.5 92-93 28.15836575204502 34.0 29.0 35.0 2.0 35.0 94-95 27.69471538797496 34.0 27.5 35.0 2.0 35.0 96-97 27.139896288863074 34.0 26.5 35.0 2.0 35.0 98-99 26.423218474205363 33.0 25.0 35.0 2.0 35.0 100-101 23.969661657090008 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3960425229844944 1101 2 0.09522880046531768 1101 3 0.22068293324279153 1101 4 0.3399885957242361 1101 5 0.32403457473078845 1101 6 0.37316969685136314 1101 7 0.26035000598277236 1101 8 0.28331988832042754 1101 9 0.4116978518981327 1101 10-11 0.41521969447108376 1101 12-13 0.37228692385770756 1101 14-15 0.37554287099315786 1101 16-17 0.4627514743283072 1101 18-19 0.5166549422038429 1101 20-21 0.5113540589248515 1101 22-23 0.5650080110391684 1101 24-25 0.4618601491512706 1101 26-27 0.36579692597259594 1101 28-29 0.3634775127118175 1101 30-31 0.45005963279546535 1101 32-33 0.4948592351829362 1101 34-35 0.3848076782736456 1101 36-37 0.4659433129714472 1101 38-39 0.6925841603133023 1101 40-41 0.5015982366456768 1101 42-43 0.3263812422239454 1101 44-45 0.4399337279995592 1101 46-47 0.4549284790119543 1101 48-49 0.40940826813208275 1101 50-51 0.4819709535017225 1101 52-53 0.5574517505288625 1101 54-55 0.5999202706935023 1101 56-57 0.5741728913908304 1101 58-59 0.465578595906436 1101 60-61 0.47383006110435133 1101 62-63 0.48562338616810763 1101 64-65 0.47785127915304315 1101 66-67 0.4549875174865079 1101 68-69 0.42045709450732716 1101 70-71 0.3417838190500362 1101 72-73 0.40887686636092724 1101 74-75 0.2991019052949362 1101 76-77 0.22229301278018454 1101 78-79 0.17266840773395487 1101 80-81 0.2639656770084393 1101 82-83 0.18695116827888825 1101 84-85 0.28601911106042976 1101 86-87 0.19820446207695852 1101 88-89 0.058109892658318785 1101 90-91 0.23020558897869137 1101 92-93 -0.03385682182408445 1101 94-95 -0.041367422659035924 1101 96-97 -0.07123621046719464 1101 98-99 -0.0866698594124351 1101 100-101 -0.17153220099043054 1102 1 0.25934852551321086 1102 2 0.4048324636403571 1102 3 0.39835620247119863 1102 4 0.3378319078922729 1102 5 0.26586857723306423 1102 6 0.22190649102797977 1102 7 0.30162461069441093 1102 8 0.3681282227403244 1102 9 0.4530373059438446 1102 10-11 0.48519033603476913 1102 12-13 0.5291781844668009 1102 14-15 0.5331898810602311 1102 16-17 0.5528623777043222 1102 18-19 0.5031899624276122 1102 20-21 0.6029981465036656 1102 22-23 0.4895697858126127 1102 24-25 0.5506028830312175 1102 26-27 0.6117946137014343 1102 28-29 0.4985857993965368 1102 30-31 0.5442168730170849 1102 32-33 0.4959147923371674 1102 34-35 0.5093381573918165 1102 36-37 0.38264461872862654 1102 38-39 0.5594199079648519 1102 40-41 0.6088692238384112 1102 42-43 0.5598252829625352 1102 44-45 0.5311710959757505 1102 46-47 0.4467239367773459 1102 48-49 0.3775398459163739 1102 50-51 0.3250028650191439 1102 52-53 0.29920325256198055 1102 54-55 0.27438521894001155 1102 56-57 0.3127164166529539 1102 58-59 0.1867084916008963 1102 60-61 0.18067506665234134 1102 62-63 0.10469323468364422 1102 64-65 0.22625858088515827 1102 66-67 0.2494951919439572 1102 68-69 0.231998665064566 1102 70-71 0.15896315114044768 1102 72-73 0.14468329536694569 1102 74-75 -0.04470234664596617 1102 76-77 -0.06486644732639135 1102 78-79 -0.07863969695958062 1102 80-81 -0.09418865862390291 1102 82-83 -0.21476333245903234 1102 84-85 -0.18694236526838992 1102 86-87 -0.23363324275779007 1102 88-89 -0.14633548100257698 1102 90-91 -0.2818308467091519 1102 92-93 -0.09592479411483268 1102 94-95 -0.10602792262674754 1102 96-97 -0.38132527985721865 1102 98-99 -0.3836880016676396 1102 100-101 -0.33042126167880426 1103 1 0.21665180614272828 1103 2 0.2967283459781207 1103 3 0.368530799519867 1103 4 0.3373722180201568 1103 5 0.27103890631184413 1103 6 0.28595670326475187 1103 7 0.31764067593011447 1103 8 0.3299786912849285 1103 9 0.3328622428563719 1103 10-11 0.3461479282594624 1103 12-13 0.3303390128379462 1103 14-15 0.4930066643198998 1103 16-17 0.4399270586224091 1103 18-19 0.482231319251639 1103 20-21 0.5344679504985521 1103 22-23 0.44781392449657886 1103 24-25 0.4908370533477324 1103 26-27 0.5167424193813517 1103 28-29 0.5150720910339004 1103 30-31 0.4412262817327175 1103 32-33 0.4013976646005162 1103 34-35 0.4600937954144726 1103 36-37 0.5000118993192402 1103 38-39 0.5416606549669751 1103 40-41 0.5240135533551324 1103 42-43 0.30912693394216006 1103 44-45 0.33176268158430844 1103 46-47 0.37099654813596317 1103 48-49 0.4426476960766621 1103 50-51 0.33608567309072157 1103 52-53 0.4731912763102386 1103 54-55 0.5972236735445264 1103 56-57 0.5076677439652428 1103 58-59 0.452134139702153 1103 60-61 0.5498464239883418 1103 62-63 0.40633718995094625 1103 64-65 0.3901803690368837 1103 66-67 0.29218272562201264 1103 68-69 0.29416351393223295 1103 70-71 0.3285693969790273 1103 72-73 0.35159567750471865 1103 74-75 0.18405955574875676 1103 76-77 0.2319471170235019 1103 78-79 0.16443223092948855 1103 80-81 0.07835011951960524 1103 82-83 0.12976916104985747 1103 84-85 0.14493839039529632 1103 86-87 0.11789820803993223 1103 88-89 0.17771237807593465 1103 90-91 0.11508967692394734 1103 92-93 0.19422286183830195 1103 94-95 0.07273078503818553 1103 96-97 -0.023193638888653112 1103 98-99 -0.18524557090108118 1103 100-101 -0.5369172482741824 1104 1 0.21527803181758998 1104 2 0.20105723286536303 1104 3 0.19835769587999863 1104 4 0.23930559569596 1104 5 0.17678754233809713 1104 6 0.21617540141691194 1104 7 0.17492834164294635 1104 8 0.21458659710350503 1104 9 0.27095930493553055 1104 10-11 0.2416421620670448 1104 12-13 0.2126869244417975 1104 14-15 0.23002096092412927 1104 16-17 0.166494653255306 1104 18-19 0.20573839787520853 1104 20-21 0.20993408366635435 1104 22-23 0.27616786856906117 1104 24-25 0.28828029366302843 1104 26-27 0.2525267920341818 1104 28-29 0.11586952613420465 1104 30-31 0.1729407111950465 1104 32-33 0.30076999768687074 1104 34-35 0.29863891005651766 1104 36-37 0.30182046774819327 1104 38-39 0.40583034657234407 1104 40-41 0.30667328620369716 1104 42-43 0.3447770158567778 1104 44-45 0.3736710036377531 1104 46-47 0.3253556703009508 1104 48-49 0.2195877974061915 1104 50-51 0.3783995633323016 1104 52-53 0.43852795977051784 1104 54-55 0.3719416154565849 1104 56-57 0.3635528522541662 1104 58-59 0.33224497937807485 1104 60-61 0.4927093681190442 1104 62-63 0.43172902755905085 1104 64-65 0.3337997486321669 1104 66-67 0.43688246124730057 1104 68-69 0.37262917159283404 1104 70-71 0.22971319304256355 1104 72-73 0.29003657388594917 1104 74-75 0.2880790699082816 1104 76-77 0.29009262327197405 1104 78-79 0.21914681785230172 1104 80-81 0.1754864356241903 1104 82-83 0.16372019357514844 1104 84-85 0.04399150458983314 1104 86-87 -0.06674654274988256 1104 88-89 -0.022375416887339128 1104 90-91 0.01854686571125086 1104 92-93 0.06783041431503634 1104 94-95 0.027018600657559233 1104 96-97 -0.023280053305104076 1104 98-99 -0.11242589388912805 1104 100-101 -0.04358203340313693 1105 1 0.23817451837987846 1105 2 0.19465019519673277 1105 3 0.2861327849080624 1105 4 0.1882058841421994 1105 5 0.22638265218665765 1105 6 0.17970122729538218 1105 7 0.20036034432532546 1105 8 0.18783324953849245 1105 9 0.21882969003899433 1105 10-11 0.3400656731338003 1105 12-13 0.307906663906202 1105 14-15 0.33077309747751116 1105 16-17 0.3272233986550859 1105 18-19 0.3291974449675479 1105 20-21 0.3120646274854053 1105 22-23 0.4029841806506269 1105 24-25 0.20961092018777805 1105 26-27 0.3221375911944335 1105 28-29 0.37253517709466166 1105 30-31 0.510813969007188 1105 32-33 0.202942260869456 1105 34-35 0.2833714682092747 1105 36-37 0.32908118394366426 1105 38-39 0.45432484122143535 1105 40-41 0.38952476994572294 1105 42-43 0.25408011796507424 1105 44-45 0.3158040599710219 1105 46-47 0.32252435239244903 1105 48-49 0.29762379738748734 1105 50-51 0.1099188394855446 1105 52-53 0.15810116600534485 1105 54-55 0.17909635359964682 1105 56-57 0.15940607895814196 1105 58-59 0.2634846182585022 1105 60-61 0.32466880188326286 1105 62-63 0.26178600273710373 1105 64-65 0.16391802005776412 1105 66-67 0.13626367246873627 1105 68-69 0.08177778188051832 1105 70-71 -0.04723481750241376 1105 72-73 0.07567999427723038 1105 74-75 0.16153258769993428 1105 76-77 0.18167978280765595 1105 78-79 0.16022507319174295 1105 80-81 0.3090976579181266 1105 82-83 0.097966318609636 1105 84-85 0.15318817871863288 1105 86-87 0.03919776512259432 1105 88-89 0.09636893769666699 1105 90-91 0.05824496968561377 1105 92-93 0.19727071132758311 1105 94-95 0.18412322121925584 1105 96-97 0.019728806340769012 1105 98-99 -0.13887516151271484 1105 100-101 -0.5314674303999745 1106 1 0.1820226644590548 1106 2 0.24786455169272514 1106 3 0.18394624073941657 1106 4 0.2502521727732656 1106 5 0.3092445077690371 1106 6 0.32154264180321235 1106 7 0.21335876431702872 1106 8 0.23322654665010845 1106 9 0.26649476104767444 1106 10-11 0.3915322149941147 1106 12-13 0.37421578385393417 1106 14-15 0.40609173696602596 1106 16-17 0.3531695605113683 1106 18-19 0.32685653118895175 1106 20-21 0.25674563098812087 1106 22-23 0.44006051853789785 1106 24-25 0.42481781837951615 1106 26-27 0.34279494946898836 1106 28-29 0.4936161042561906 1106 30-31 0.37258347803823 1106 32-33 0.38930982021695826 1106 34-35 0.428397474219004 1106 36-37 0.39545002950081454 1106 38-39 0.5039877552614342 1106 40-41 0.567495837809048 1106 42-43 0.5747323045031294 1106 44-45 0.5220419077657183 1106 46-47 0.3592031512438041 1106 48-49 0.4912773309000329 1106 50-51 0.5291691430958139 1106 52-53 0.48855067549119724 1106 54-55 0.48572219166854325 1106 56-57 0.561664966306104 1106 58-59 0.4794377503440401 1106 60-61 0.5068531844372259 1106 62-63 0.5753867772015795 1106 64-65 0.5752314198314039 1106 66-67 0.42239800239446623 1106 68-69 0.5059056565498992 1106 70-71 0.454833571438094 1106 72-73 0.44800870984896335 1106 74-75 0.40940694898130303 1106 76-77 0.40310193454102716 1106 78-79 0.46373338116461227 1106 80-81 0.4800604726436646 1106 82-83 0.41397953086809736 1106 84-85 0.5091778748589384 1106 86-87 0.5932648468241695 1106 88-89 0.5238330103278983 1106 90-91 0.7238929387520265 1106 92-93 0.6502090095768587 1106 94-95 0.6123416582520171 1106 96-97 0.6247971570128676 1106 98-99 0.6261028537166418 1106 100-101 0.4991519394526627 1107 1 0.13191737159674943 1107 2 0.11086331122404758 1107 3 0.09005887059179685 1107 4 0.19569577260160287 1107 5 0.15089003943626267 1107 6 0.023930808555128635 1107 7 0.07857414142538488 1107 8 0.051953978644789345 1107 9 0.16505134934728716 1107 10-11 0.1989029505156097 1107 12-13 0.09842338304733289 1107 14-15 0.18170700354921365 1107 16-17 0.270202136041803 1107 18-19 0.2930285389257108 1107 20-21 0.26239058651350433 1107 22-23 0.29024794100860873 1107 24-25 0.1679851875865026 1107 26-27 0.23223879621525612 1107 28-29 0.1647984296352405 1107 30-31 0.27771455030875103 1107 32-33 0.3499366390321086 1107 34-35 0.41115648819339157 1107 36-37 0.40989972585054346 1107 38-39 0.45979009157541384 1107 40-41 0.2928279130367173 1107 42-43 0.2820443676200597 1107 44-45 0.44205284270284295 1107 46-47 0.4460002728861667 1107 48-49 0.4659012807639371 1107 50-51 0.46186389961710006 1107 52-53 0.47844391287927834 1107 54-55 0.4586229482956412 1107 56-57 0.5159683343843184 1107 58-59 0.5337719831641365 1107 60-61 0.4880574972704892 1107 62-63 0.5241113284870238 1107 64-65 0.5106664951119768 1107 66-67 0.32648884467857187 1107 68-69 0.41884450908698057 1107 70-71 0.2988767350447361 1107 72-73 0.20646758919427555 1107 74-75 0.4186900051198599 1107 76-77 0.31078948928919914 1107 78-79 0.344253319787871 1107 80-81 0.32569378775210467 1107 82-83 0.4724981408377005 1107 84-85 0.43524394304356306 1107 86-87 0.37829444758138564 1107 88-89 0.28184291056129496 1107 90-91 0.3616357560856649 1107 92-93 0.4188350876382003 1107 94-95 0.22285995075570852 1107 96-97 0.27853913952872844 1107 98-99 0.30384608258017565 1107 100-101 0.45537227255671553 1108 1 0.04086974828438983 1108 2 0.04823876741821209 1108 3 0.00856165813405596 1108 4 0.11014442137127389 1108 5 0.1993245377906021 1108 6 0.17703337667639119 1108 7 0.17377240396932336 1108 8 0.16612969456176785 1108 9 0.20457802691037585 1108 10-11 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-0.9686604049006391 2316 70-71 -1.015270435952651 2316 72-73 -1.2004076776153134 2316 74-75 -1.293975276091583 2316 76-77 -0.9750344421823698 2316 78-79 -1.112460430464278 2316 80-81 -0.9562522451704432 2316 82-83 -0.894288181623093 2316 84-85 -0.768008248899843 2316 86-87 -0.7166638714830107 2316 88-89 -0.8949002532492365 2316 90-91 -1.000125398314406 2316 92-93 -1.0113107135411568 2316 94-95 -1.0782402664448902 2316 96-97 -0.982793759090665 2316 98-99 -0.9097211675101313 2316 100-101 -0.5503852321227107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8731.0 3 2577.0 4 2621.0 5 2833.0 6 3277.0 7 3938.0 8 5071.0 9 6243.0 10 7672.0 11 9727.0 12 12118.0 13 16847.0 14 15697.0 15 16865.0 16 20487.0 17 26275.0 18 22624.0 19 17527.0 20 17426.0 21 18596.0 22 20007.0 23 22490.0 24 25291.0 25 29042.0 26 33578.0 27 39072.0 28 45680.0 29 55019.0 30 66092.0 31 81191.0 32 101011.0 33 130320.0 34 178553.0 35 257682.0 36 399111.0 37 617552.0 38 578116.0 39 98515.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29098181571493 18.45441225472232 13.820648584584095 23.433957344978655 2 15.40803270927823 20.030415963700033 40.30447169774991 24.25707962927183 3 19.765426233203883 24.110346485361053 29.225835518808367 26.8983917626267 4 12.833470013845128 15.703561551052662 36.82963189226698 34.63333654283523 5 13.77625881436516 37.56263482820185 33.809940754450224 14.851165602982764 6 32.74644802941118 35.273407170965754 17.34011984790067 14.640024951722399 7 29.241661622758112 32.59430117974366 20.211671604387668 17.952365593110553 8 26.722068426770985 34.72277991970484 19.720964683534977 18.834186969989204 9 26.968649347494676 16.735634960719597 20.081122642329653 36.21459304945607 10-11 25.41329289928115 26.292531120676166 26.990443257578107 21.303732722464574 12-13 26.138396160735677 23.570762045651993 27.67324242225138 22.617599371360953 14-15 22.825688584832925 25.078306817384306 26.04929642077411 26.046708177008664 16-17 22.2820935478171 28.426471064020763 26.789803200001327 22.50163218816081 18-19 22.30468770092215 26.888985529706073 29.063918277044298 21.74240849232748 20-21 23.03128865118943 26.056812913558392 29.036457685171452 21.875440750080724 22-23 22.632932078519406 25.98824230080122 29.00327056332047 22.375555057358902 24-25 22.386374876113813 26.212259106505943 29.19253091595123 22.208835101429013 26-27 22.09169154776266 26.468374662422818 29.096485546228223 22.3434482435863 28-29 21.98921410457121 26.8703312867635 28.982206823809687 22.158247784855607 30-31 22.280854769613914 26.416888695954622 28.88705579207704 22.415200742354426 32-33 21.99485087584226 26.510041389453786 28.90121818799679 22.593889546707164 34-35 22.587513587001684 26.66710433155276 28.62364919441961 22.12173288702595 36-37 22.71771740040226 26.201318945223225 28.771982489721687 22.308981164652828 38-39 22.296049840712676 26.54997795762874 28.77153290135831 22.382439300300273 40-41 22.532992949816386 26.50202709828042 28.75891419254889 22.20606575935431 42-43 22.279667581474214 26.532358100572047 28.52604073777321 22.66193358018053 44-45 22.31176426347978 26.75460285619015 28.541984610889514 22.391648269440548 46-47 22.64113958657605 26.95490794922406 27.963690870201734 22.440261593998155 48-49 22.484959347969927 26.928941156735643 27.946856140939463 22.639243354354967 50-51 22.50607547770128 27.18550791392957 27.89790801217558 22.410508596193566 52-53 22.791919013747595 27.07328417158742 27.726143060514207 22.40865375415078 54-55 22.255025901114877 27.099451163245742 27.94490202100907 22.700620914630314 56-57 22.4165484623345 27.29993580618959 27.716205801463634 22.56730993001228 58-59 22.563135494341893 26.87251750439554 27.831976431167394 22.732370570095174 60-61 22.365210330456705 27.062592469870555 28.23939029031238 22.332806909360368 62-63 22.276333599971252 27.05911242847693 28.285219829875246 22.379334141676573 64-65 22.067076758812032 27.06371849421939 28.11431612552787 22.754888621440706 66-67 22.032540972448047 27.234616463802887 27.872132078417067 22.860710485331996 68-69 22.225236676753624 26.952863048466824 28.098601735094135 22.723298539685413 70-71 22.282983465636757 26.938583726154636 27.89761640789922 22.880816400309385 72-73 22.22472217141889 27.005092851386024 27.948376197014785 22.8218087801803 74-75 22.101074403780707 27.46946530775338 27.682568676950105 22.746891611515803 76-77 22.15458602638006 27.075708592363064 27.770660659556352 22.999044721700525 78-79 22.33297160574277 27.14219620829853 27.7425819364565 22.7822502495022 80-81 22.407577864036902 27.23078205322931 27.580702125921654 22.780937956812135 82-83 22.328460729973738 27.230951815650243 27.704291536937355 22.736295917438664 84-85 22.28222508934428 27.26570401028437 27.587506476815417 22.86456442355594 86-87 22.246453058176634 27.12413863787318 27.704131343665022 22.925276960285164 88-89 22.20131048373677 27.107437301393954 27.581212982894755 23.110039231974525 90-91 22.200612182736048 27.063819800566247 27.72520351996751 23.010364496730197 92-93 22.396122950973833 26.834680335357152 27.734954473667262 23.034242240001756 94-95 22.2139892093162 26.766884181314243 27.878890161011437 23.14023644835812 96-97 22.26087995716376 26.473015881998606 28.023699079743214 23.242405081094414 98-99 22.235756432152222 26.626432395206773 27.68595632756952 23.45185484507148 100-101 22.29326769964735 26.168476063416463 28.193460747077914 23.34479548985827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2323.0 1 1375.5 2 534.0 3 660.5 4 604.5 5 588.0 6 971.0 7 1884.0 8 2898.0 9 3322.5 10 3081.0 11 2881.5 12 3021.0 13 3301.5 14 3576.0 15 3783.0 16 4053.0 17 4337.0 18 4574.0 19 4900.0 20 5288.0 21 5930.5 22 7025.5 23 8502.0 24 10321.0 25 12659.5 26 16133.5 27 20614.0 28 24611.5 29 28779.0 30 33854.0 31 40151.0 32 48098.5 33 53346.0 34 56141.5 35 60303.5 36 67483.5 37 74495.5 38 78353.0 39 81470.0 40 85190.5 41 91505.0 42 96277.0 43 99629.5 44 104183.5 45 109332.5 46 111491.0 47 114750.5 48 123195.5 49 122104.5 50 113867.5 51 108545.0 52 105540.0 53 105395.5 54 103557.5 55 95729.0 56 85695.0 57 77415.0 58 72087.5 59 63911.5 60 52314.5 61 43322.5 62 36276.5 63 27711.0 64 19869.5 65 15327.0 66 11559.0 67 9258.0 68 7200.0 69 4902.5 70 3467.0 71 2414.5 72 1835.0 73 1482.5 74 1146.5 75 932.0 76 668.5 77 381.0 78 240.5 79 183.5 80 130.0 81 97.0 82 72.0 83 60.5 84 45.5 85 28.5 86 19.0 87 12.0 88 10.0 89 11.5 90 7.5 91 7.0 92 4.0 93 5.0 94 5.0 95 3.5 96 4.5 97 3.0 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2781968176990082 2 0.10800894448035163 3 0.11805705936446172 4 0.11941670527287264 5 0.12561801709903958 6 0.055413861291577396 7 0.06483189636447266 8 0.06993885904484544 9 0.06728589141867775 10-11 0.06166491626073498 12-13 0.06622470436821068 14-15 0.061830726737370466 16-17 0.06295823797849173 18-19 0.06770041761026645 20-21 0.07169645009718152 22-23 0.07963877192802102 24-25 0.08771374214016887 26-27 0.08826091671306598 28-29 0.08230832060185224 30-31 0.07875997640185296 32-33 0.07172961219250862 34-35 0.23548403891770858 36-37 0.3562106469560015 38-39 0.3288353372634838 40-41 0.314045042747599 42-43 0.33569989099619263 44-45 0.3485004597924517 46-47 0.35423750228403933 48-49 0.3636886994522617 50-51 0.3577858464840386 52-53 0.35760345495973955 54-55 0.34515108816441503 56-57 0.3233138483915223 58-59 0.30898782321021684 60-61 0.2683642564345243 62-63 0.3371590231905849 64-65 0.3635560510709533 66-67 0.3679002855588029 68-69 0.3795899241616042 70-71 0.38051846283076285 72-73 0.36430219821581294 74-75 0.36745259727188706 76-77 0.3517669261821707 78-79 0.33183650689058597 80-81 0.32987994326628733 82-83 0.34341007815974245 84-85 0.3495948089382459 86-87 0.34379144225600405 88-89 0.35811746743730954 90-91 0.3808998269270245 92-93 0.37640636301020297 94-95 0.37557731062702554 96-97 0.3811153805466506 98-99 0.3704206048036621 100-101 0.34919686379432074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3015491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.17128535536225 #Duplication Level Percentage of deduplicated Percentage of total 1 73.39161880742351 36.08760231071793 2 13.748106412377092 13.520241269977593 3 5.200588149555918 7.67158811752588 4 2.422598894658866 4.764892062034251 5 1.3505722271610832 3.320468618738237 6 0.8286504294188124 2.4447484034877536 7 0.540997984913144 1.8621096404988116 8 0.39314769631208174 1.5465262049731738 9 0.30080503971617245 1.3311873399793492 >10 1.6353237926476694 14.81408000964447 >50 0.12221070277647943 4.155084832381195 >100 0.05970039221766391 5.1623178240802075 >500 0.0039215393767884415 1.3761540454371537 >1k 0.0016227059489350426 1.3956694809573975 >5k 1.3522549574458687E-4 0.547329839566499 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9425 0.31255274845787967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7038 0.23339482691210156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4828 0.1601065962392194 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3487 0.1156362264055837 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.632419065419198E-5 2 0.0 0.0 0.0 0.0 6.632419065419198E-5 3 0.0 0.0 0.0 0.0 6.632419065419198E-5 4 0.0 0.0 0.0 0.0 6.632419065419198E-5 5 0.0 0.0 0.0 0.0 9.948628598128796E-5 6 0.0 0.0 0.0 0.0 1.6581047663547993E-4 7 0.0 0.0 0.0 0.0 1.6581047663547993E-4 8 0.0 0.0 0.0 0.0 1.6581047663547993E-4 9 0.0 0.0 0.0 0.0 1.6581047663547993E-4 10-11 0.0 0.0 0.0 0.0 1.6581047663547993E-4 12-13 0.0 0.0 0.0 1.6581047663547994E-5 6.134987635512757E-4 14-15 0.0 0.0 0.0 4.9743142990643985E-5 6.632419065419196E-4 16-17 0.0 0.0 0.0 9.948628598128796E-5 6.964040018690157E-4 18-19 0.0 0.0 0.0 9.948628598128796E-5 6.964040018690157E-4 20-21 0.0 0.0 0.0 2.652967626167679E-4 7.793092401867556E-4 22-23 0.0 0.0 0.0 5.471745728970837E-4 8.124713355138517E-4 24-25 0.0 0.0 0.0 8.953765738315916E-4 8.622144785044956E-4 26-27 0.0 0.0 0.0 0.0012269975271025513 8.953765738315916E-4 28-29 0.0 0.0 0.0 0.002885102293457351 9.119576214951396E-4 30-31 0.0 0.0 0.0 0.007958902878503036 9.451197168222356E-4 32-33 0.0 0.0 0.0 0.019383244718687603 9.948628598128795E-4 34-35 0.0 0.0 0.0 0.036362237526160746 0.0011275112411212635 36-37 0.0 0.0 0.0 0.06705375675138808 0.0011275112411212635 38-39 0.0 0.0 0.0 0.1141107700205373 0.0011606733364483595 40-41 0.0 0.0 0.0 0.16597628711211543 0.0011606733364483595 42-43 0.0 0.0 0.0 0.2227000511691131 0.0011938354317754555 44-45 0.0 0.0 0.0 0.29033414458872536 0.0012601596224296476 46-47 0.0 0.0 0.0 0.37197922328403565 0.0013099027654202913 48-49 0.0 0.0 0.0 0.46290968867093285 0.0013928080037380313 50-51 0.0 0.0 0.0 0.5631089597017533 0.0014259700990651273 52-53 0.0 0.0 0.0 0.6654803479764987 0.0015751995280370592 54-55 0.0 0.0 0.0 0.7823269908615214 0.0016249426710277033 56-57 0.0 0.0 0.0 0.9232327339063522 0.0016581047663547994 58-59 0.0 0.0 0.0 1.077718354987629 0.0016581047663547994 60-61 0.0 0.0 0.0 1.249249293067033 0.0016581047663547994 62-63 0.0 0.0 0.0 1.4229191862950346 0.0016912668616818952 64-65 0.0 0.0 0.0 1.6082455560305104 0.0017741720999996354 66-67 0.0 0.0 0.0 1.8254075372799985 0.0017907531476631833 68-69 0.0 0.0 0.0 2.073227875659387 0.0017907531476631833 70-71 0.0 0.0 0.0 2.350264020021947 0.0017907531476631833 72-73 0.0 0.0 0.0 2.633518057258337 0.0018736583859809233 74-75 0.0 0.0 0.0 2.9272347355704262 0.001973144671962211 76-77 0.0 0.0 0.0 3.252040878251668 0.001989725719625759 78-79 0.0 0.0 0.0 3.620189879525424 0.002072630957943499 80-81 0.0 0.0 0.0 4.026127088424406 0.0021223741009341428 82-83 0.0 0.0 0.0 4.4419134396355355 0.002188698291588335 84-85 0.0 0.0 0.0 4.864133237340122 0.002188698291588335 86-87 0.0 0.0 0.0 5.31790676874844 0.002304765625233171 88-89 0.0 0.0 0.0 5.81001568235488 0.002470576101868651 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14880 0.0 31.4456 1 GGTATCA 9930 0.0 27.841993 1 GTACATG 13735 0.0 27.21903 1 TACATGG 13555 0.0 26.698551 2 ATCAACG 17400 0.0 26.577738 3 TATCAAC 17595 0.0 26.256231 2 TCAACGC 17680 0.0 26.237309 4 CAACGCA 17690 0.0 26.141605 5 AACGCAG 18115 0.0 25.563257 6 ACATGGG 13840 0.0 25.291988 3 CATGGGG 6650 0.0 23.10926 4 ACGCAGA 20110 0.0 22.791954 7 CGCAGAG 20375 0.0 22.495516 8 GAGTACT 9515 0.0 22.218874 12-13 GCAGAGT 21595 0.0 20.873116 9 TACCTGG 2140 0.0 20.612556 2 GTACTTT 10265 0.0 20.387844 14-15 AGTACTT 10190 0.0 20.165586 12-13 AGAGTAC 16685 0.0 19.843164 10-11 GTACCGT 220 1.3742447E-6 19.390087 6 >>END_MODULE