FastQCFastQC Report
Fri 27 May 2016
SRR939328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939328_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015491
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98180.3255854519214284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78220.2593939096485448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.1791084768616454No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38980.12926584758502016No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT902.4305216E-531.6355676
GTATCAA155550.028.7789671
GGTATCA109800.028.1188771
GTACATG136500.025.1628681
TACATGG138000.024.3112432
ACATGGG138100.023.7094943
ATCAACG190400.023.4275993
TATCAAC191200.023.3792112
TCAACGC191950.023.2878654
CAACGCA192950.023.1179835
AACGCAG198800.022.509316
GAGTACT106800.021.19545712-13
CATGGGG66400.020.9395054
TAAGGTG14200.020.384895
ACGCAGA221800.020.0253987
GTACTTT112900.019.84009414-15
CGCAGAG224100.019.7351728
AGTACTT113300.019.20459212-13
AGAGTAC183050.019.15873310-11
CATGGGA67450.018.995414
Produced by FastQC (version 0.11.5)