##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939328_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3015491 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25511301476277 33.0 31.0 34.0 31.0 34.0 2 32.44849047800176 34.0 31.0 34.0 31.0 34.0 3 32.540501364454414 34.0 31.0 34.0 31.0 34.0 4 36.08575353068539 37.0 35.0 37.0 35.0 37.0 5 36.020470629824466 37.0 35.0 37.0 35.0 37.0 6 36.059495120363486 37.0 35.0 37.0 35.0 37.0 7 36.00593203561211 37.0 35.0 37.0 35.0 37.0 8 35.98047813772285 37.0 35.0 37.0 35.0 37.0 9 37.684799921472155 39.0 37.0 39.0 35.0 39.0 10-11 37.60196399193365 39.0 37.0 39.0 35.0 39.0 12-13 37.52109159005946 39.0 37.0 39.0 35.0 39.0 14-15 38.81316359425381 40.0 38.0 41.0 35.0 41.0 16-17 38.75865489235418 40.0 38.0 41.0 34.0 41.0 18-19 38.87249970237019 40.0 38.0 41.0 35.0 41.0 20-21 38.871992653932644 40.0 38.0 41.0 35.0 41.0 22-23 38.819480144361236 40.0 38.0 41.0 34.0 41.0 24-25 38.74969233866061 40.0 38.0 41.0 34.0 41.0 26-27 38.58261705970935 40.0 38.0 41.0 34.0 41.0 28-29 38.4311934607001 40.0 38.0 41.0 34.0 41.0 30-31 38.23878814428562 40.0 38.0 41.0 34.0 41.0 32-33 38.031138544270235 40.0 37.0 41.0 33.0 41.0 34-35 37.9839032847387 40.0 37.0 41.0 33.0 41.0 36-37 37.90037476483929 40.0 37.0 41.0 33.0 41.0 38-39 37.79114744497662 40.0 37.0 41.0 33.0 41.0 40-41 37.61187564479549 40.0 37.0 41.0 33.0 41.0 42-43 37.42977760504011 40.0 37.0 41.0 32.0 41.0 44-45 37.203214335575865 40.0 36.5 41.0 31.5 41.0 46-47 36.91770842625628 40.0 36.0 41.0 31.0 41.0 48-49 36.6010538582274 39.5 36.0 41.0 30.5 41.0 50-51 36.34815723210582 39.0 35.5 41.0 30.0 41.0 52-53 36.11410032396051 39.0 35.0 41.0 29.5 41.0 54-55 35.8306491380674 39.0 35.0 40.0 29.0 41.0 56-57 35.57829753098252 38.5 35.0 40.0 28.0 41.0 58-59 35.27288192868093 38.0 34.5 40.0 28.0 41.0 60-61 34.93668493787579 38.0 34.0 40.0 27.0 41.0 62-63 34.55488277033491 37.0 34.0 40.0 26.0 41.0 64-65 34.12906588015019 36.5 33.5 39.5 26.0 41.0 66-67 33.75557230978305 36.0 33.0 39.0 26.0 40.5 68-69 33.3694133061581 36.0 33.0 39.0 25.0 40.0 70-71 32.971253603476185 35.0 32.5 38.5 24.5 40.0 72-73 32.480285465948995 35.0 32.0 37.5 23.0 39.5 74-75 31.983859842393827 35.0 31.5 37.0 22.0 39.0 76-77 29.997855075674245 33.0 29.5 35.0 19.0 37.5 78-79 30.98823093817889 34.0 31.0 36.0 20.0 38.0 80-81 31.047356964421382 34.0 31.0 36.0 20.0 37.0 82-83 30.73813568006006 34.0 31.0 35.0 18.5 37.0 84-85 30.36456799240986 34.0 31.0 35.0 17.0 36.5 86-87 29.836636554378707 34.0 30.0 35.0 9.0 36.0 88-89 29.332909466484892 34.0 29.0 35.0 4.5 36.0 90-91 28.91012193370831 34.0 29.0 35.0 2.0 35.0 92-93 28.559474891485333 34.0 29.0 35.0 2.0 35.0 94-95 28.155461084115323 34.0 29.0 35.0 2.0 35.0 96-97 27.593934287981625 33.0 27.5 35.0 2.0 35.0 98-99 26.8374292942675 33.0 26.5 35.0 2.0 35.0 100-101 23.47271903646869 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21798329401382688 1101 2 0.22327829863266402 1101 3 0.2202964163741754 1101 4 0.15205648895874901 1101 5 0.14584191059419283 1101 6 0.14903231443333453 1101 7 0.15935485546494021 1101 8 0.1896929816070596 1101 9 0.22354415268213046 1101 10-11 0.1899530504281799 1101 12-13 0.22775235896640567 1101 14-15 0.31404932833844157 1101 16-17 0.284225966709883 1101 18-19 0.1983391523648308 1101 20-21 0.30654616472955354 1101 22-23 0.30789052512652404 1101 24-25 0.25685707457077456 1101 26-27 0.2776445440611326 1101 28-29 0.2590450055533182 1101 30-31 0.2764919429332977 1101 32-33 0.2997387772943654 1101 34-35 0.3145378957926894 1101 36-37 0.37485504982547724 1101 38-39 0.35554696275846 1101 40-41 0.5330791045612386 1101 42-43 0.5306196446616056 1101 44-45 0.6835975120585971 1101 46-47 0.5436496256031944 1101 48-49 0.5666613640028189 1101 50-51 0.6345428065821679 1101 52-53 0.5945879816868782 1101 54-55 0.5057347137402317 1101 56-57 0.5868508940922084 1101 58-59 0.5231235114994988 1101 60-61 0.456559237928829 1101 62-63 0.45764202323884007 1101 64-65 0.46030882736263123 1101 66-67 0.4614265062564513 1101 68-69 0.42223891287575555 1101 70-71 0.4768151392125546 1101 72-73 0.44849256775756174 1101 74-75 0.3556811069548189 1101 76-77 0.498896394558102 1101 78-79 0.4896905129041045 1101 80-81 0.5245721416665141 1101 82-83 0.3533377203044239 1101 84-85 0.3512878996238946 1101 86-87 0.4147088627297748 1101 88-89 0.6500622535098763 1101 90-91 0.7353237287090941 1101 92-93 0.6239536224478144 1101 94-95 0.744350763185814 1101 96-97 0.612272688197848 1101 98-99 0.5082892532633068 1101 100-101 0.28696188449751503 1102 1 0.145440020163349 1102 2 0.24868767210135445 1102 3 0.2305062624857399 1102 4 0.10967435124657499 1102 5 0.14743161422723006 1102 6 0.13702747730636133 1102 7 0.20815418587835666 1102 8 0.2098835670533532 1102 9 0.3150908974176687 1102 10-11 0.2709433975153033 1102 12-13 0.27472682085805644 1102 14-15 0.28557337993758125 1102 16-17 0.3637927951877984 1102 18-19 0.2688848326625717 1102 20-21 0.33853788329060563 1102 22-23 0.21489823228399985 1102 24-25 0.2737612720024316 1102 26-27 0.2894681885689252 1102 28-29 0.3686541485659305 1102 30-31 0.4096499994855023 1102 32-33 0.2903790632702936 1102 34-35 0.45363489841791704 1102 36-37 0.4353826094844493 1102 38-39 0.336516023843366 1102 40-41 0.43595882804697794 1102 42-43 0.442900112111019 1102 44-45 0.4663421231271343 1102 46-47 0.4962656215795107 1102 48-49 0.32458919509274864 1102 50-51 0.2572351994799149 1102 52-53 0.27889316237764206 1102 54-55 0.1733131032985824 1102 56-57 0.1802111483897164 1102 58-59 0.2535663085352624 1102 60-61 0.19193306267233368 1102 62-63 0.13218923789110448 1102 64-65 0.18948754427932357 1102 66-67 0.18259213104261818 1102 68-69 0.3413954061597195 1102 70-71 0.2007215777325655 1102 72-73 0.4067389591537207 1102 74-75 0.3189513941602655 1102 76-77 0.44148463199037735 1102 78-79 0.48074454038507497 1102 80-81 0.4994242307995158 1102 82-83 0.39152599237808516 1102 84-85 0.22120605590913556 1102 86-87 0.34628558685809097 1102 88-89 0.48656813283862377 1102 90-91 0.6222512044356847 1102 92-93 0.5936321528710309 1102 94-95 0.5786758599194002 1102 96-97 0.3767439685481264 1102 98-99 0.290563672238747 1102 100-101 0.08635886830128925 1103 1 0.17196019484820368 1103 2 0.1262646809616328 1103 3 0.17635579517038735 1103 4 0.09824275703048357 1103 5 0.1346333780963107 1103 6 0.16364099483624273 1103 7 0.1246196340989485 1103 8 0.13486766432335173 1103 9 0.2099590266717044 1103 10-11 0.17764168547784465 1103 12-13 0.21981117185578825 1103 14-15 0.2821367152192735 1103 16-17 0.30542494684033983 1103 18-19 0.24839783426723017 1103 20-21 0.2844085565761887 1103 22-23 0.2423537265911051 1103 24-25 0.32533751655906684 1103 26-27 0.3207709345371157 1103 28-29 0.2728957365992457 1103 30-31 0.2919531146374723 1103 32-33 0.2460624875667463 1103 34-35 0.34106484569338846 1103 36-37 0.45321055254991194 1103 38-39 0.4138184885672658 1103 40-41 0.48107928692094504 1103 42-43 0.48393260014902495 1103 44-45 0.6104163316345108 1103 46-47 0.6263354990329191 1103 48-49 0.43167126938960365 1103 50-51 0.5953785035761783 1103 52-53 0.47583798757053586 1103 54-55 0.38495280748146143 1103 56-57 0.5367123289538185 1103 58-59 0.3086194727563978 1103 60-61 0.2335343206799294 1103 62-63 0.28556949840544377 1103 64-65 0.39233444701918074 1103 66-67 0.3179724869260596 1103 68-69 0.28917758151269624 1103 70-71 0.3286619280073282 1103 72-73 0.4814361318651379 1103 74-75 0.4050769866205428 1103 76-77 0.5102710142553981 1103 78-79 0.5227725278390416 1103 80-81 0.43776932294484183 1103 82-83 0.39118159645421 1103 84-85 0.3956360060213697 1103 86-87 0.2644330974611506 1103 88-89 0.47095630561122803 1103 90-91 0.5605660378295134 1103 92-93 0.45837033294491647 1103 94-95 0.49432020179628466 1103 96-97 0.24514318738603436 1103 98-99 -0.06574717537507269 1103 100-101 -0.0932318874880238 1104 1 0.07940494985779623 1104 2 0.04398252766805655 1104 3 0.11153651721460989 1104 4 0.06268664346774244 1104 5 0.03552235907953616 1104 6 0.05475505313985707 1104 7 0.11443184977630239 1104 8 0.05924782159368647 1104 9 0.07370283260745936 1104 10-11 0.1816194836801941 1104 12-13 0.15342282429609355 1104 14-15 0.09797189980945831 1104 16-17 0.09526762571589842 1104 18-19 0.0636025257299977 1104 20-21 0.09875769973199766 1104 22-23 0.045160521538349485 1104 24-25 0.07494975872729981 1104 26-27 0.12072292797509476 1104 28-29 0.09981736334705005 1104 30-31 0.08372699617639512 1104 32-33 0.05457348056150835 1104 34-35 0.10780902268366077 1104 36-37 0.17637418759219514 1104 38-39 0.1445193882800453 1104 40-41 0.2018900664388923 1104 42-43 0.32305565597117436 1104 44-45 0.34983323554467916 1104 46-47 0.4145284244598173 1104 48-49 0.47618752252822816 1104 50-51 0.28707817421156534 1104 52-53 0.3397623716350111 1104 54-55 0.28652802442592673 1104 56-57 0.28022893595525034 1104 58-59 0.21745421700423861 1104 60-61 0.17644154650104582 1104 62-63 0.22071551141879553 1104 64-65 0.12007283652275902 1104 66-67 0.20216798694253413 1104 68-69 0.2524098427559096 1104 70-71 0.21162366198455373 1104 72-73 0.1905219663216826 1104 74-75 0.08651131485492414 1104 76-77 0.20253749052815806 1104 78-79 0.30512326565666115 1104 80-81 0.19323475802482548 1104 82-83 0.3324052714776542 1104 84-85 0.2485487709610048 1104 86-87 0.1315469353982408 1104 88-89 0.3488175051735709 1104 90-91 0.5558859841132175 1104 92-93 0.5066185557839269 1104 94-95 0.6029614871530988 1104 96-97 0.48957733706101436 1104 98-99 0.4392931880369453 1104 100-101 0.18910999399150796 1105 1 0.09735602989135828 1105 2 0.09625710503043194 1105 3 0.08453735273933916 1105 4 0.11208813300446963 1105 5 0.13886972820035481 1105 6 0.12160360325611208 1105 7 0.1533006218405859 1105 8 0.13118699347253227 1105 9 0.1405042414859139 1105 10-11 0.12866489712211404 1105 12-13 0.1260384413414073 1105 14-15 0.2626384551739349 1105 16-17 0.12655038682243713 1105 18-19 0.21911116894324323 1105 20-21 0.177668845829011 1105 22-23 0.18186695156526866 1105 24-25 0.18189723972325567 1105 26-27 0.14675645537950288 1105 28-29 0.16808912386247954 1105 30-31 0.09412694724050397 1105 32-33 0.09385477005256604 1105 34-35 0.1678356796002234 1105 36-37 0.12314635879248925 1105 38-39 0.15344646002686346 1105 40-41 0.12223647744249888 1105 42-43 0.11314179046929951 1105 44-45 0.2389131257877466 1105 46-47 0.21321018862396102 1105 48-49 0.29662142918579804 1105 50-51 0.3291997548762282 1105 52-53 0.3494011117734246 1105 54-55 0.2997634008790442 1105 56-57 0.2682567677376184 1105 58-59 0.25412302250972374 1105 60-61 0.23328496921779163 1105 62-63 0.17945454270806493 1105 64-65 0.2509452540075827 1105 66-67 0.20554724275047676 1105 68-69 0.2852630516397454 1105 70-71 0.39716879352693724 1105 72-73 0.2828675436963124 1105 74-75 0.26116233383237386 1105 76-77 0.2801709574199087 1105 78-79 0.38640000456484813 1105 80-81 0.42830529863118727 1105 82-83 0.42660749323281877 1105 84-85 0.3129096413070549 1105 86-87 0.44245723775556556 1105 88-89 0.625519130080221 1105 90-91 0.714617419723151 1105 92-93 0.5604676252121017 1105 94-95 0.5887866351203428 1105 96-97 0.5213842758720659 1105 98-99 0.7900750564299841 1105 100-101 0.5958364419826552 1106 1 0.14064872168973608 1106 2 0.10629088515044316 1106 3 0.0900883499124916 1106 4 0.07365821840903664 1106 5 0.07733694532073798 1106 6 0.11148876258533136 1106 7 0.09008977552319664 1106 8 0.10895439517378236 1106 9 0.1276719772837751 1106 10-11 0.18219163628356938 1106 12-13 0.11059408341861143 1106 14-15 0.18406528959069846 1106 16-17 0.160747913656472 1106 18-19 0.043930699180840804 1106 20-21 0.12850005881151105 1106 22-23 0.14435260549834084 1106 24-25 0.11571704639811742 1106 26-27 0.27341087615663184 1106 28-29 0.2156730731887606 1106 30-31 0.25757974035111175 1106 32-33 0.29273291892791065 1106 34-35 0.2815342280050501 1106 36-37 0.33929988038436676 1106 38-39 0.32458105465258313 1106 40-41 0.23129476780334812 1106 42-43 0.25786452242748226 1106 44-45 0.32255440974284966 1106 46-47 0.2929454054963827 1106 48-49 0.22692946783713097 1106 50-51 0.2899824036636076 1106 52-53 0.3479818185554038 1106 54-55 0.407731225097713 1106 56-57 0.38095073995035733 1106 58-59 0.38729150827051484 1106 60-61 0.25478796142802906 1106 62-63 0.42330112381733187 1106 64-65 0.3438616744665737 1106 66-67 0.28131856201155614 1106 68-69 0.45298381112453967 1106 70-71 0.552507034263563 1106 72-73 0.5056128285825849 1106 74-75 0.5676838813284064 1106 76-77 0.6031628351038165 1106 78-79 0.8307455787749305 1106 80-81 0.7486829429871271 1106 82-83 0.7995377441133869 1106 84-85 0.8260767234854569 1106 86-87 0.7680796097829017 1106 88-89 0.9885897037431697 1106 90-91 0.9421819560867561 1106 92-93 0.9590289654914557 1106 94-95 0.8756655483856761 1106 96-97 0.8628879136323953 1106 98-99 1.077877179678456 1106 100-101 0.8811841911341176 1107 1 0.026094790697413828 1107 2 0.006410346399029265 1107 3 0.03176210077229058 1107 4 0.04833972366780159 1107 5 -0.007446325401261333 1107 6 0.05533092323484823 1107 7 -0.005422784956998328 1107 8 0.051533982115344656 1107 9 0.02430319358976618 1107 10-11 0.08656384743368051 1107 12-13 0.01630411945686916 1107 14-15 0.07201216696067547 1107 16-17 -0.02893350341731349 1107 18-19 0.07534095332994895 1107 20-21 0.10301771638336987 1107 22-23 0.0084558524691829 1107 24-25 0.05636579387749663 1107 26-27 0.07375609094584235 1107 28-29 -0.02564446915472729 1107 30-31 0.02423370838112504 1107 32-33 0.03113424287836608 1107 34-35 0.0968563089353438 1107 36-37 0.12892585850273264 1107 38-39 0.01901799357567313 1107 40-41 0.033713270187277544 1107 42-43 0.0599297557943288 1107 44-45 -2.9414137797800777E-4 1107 46-47 -0.02168989083452999 1107 48-49 0.059107225234377836 1107 50-51 0.08209813762716323 1107 52-53 0.10433201873530606 1107 54-55 -0.005563424425794494 1107 56-57 -0.05885207844150386 1107 58-59 -0.009701619180283672 1107 60-61 -0.07732871317528378 1107 62-63 -0.06398248494707559 1107 64-65 -0.06411577369535593 1107 66-67 -0.2547423326544376 1107 68-69 -0.18414077073170176 1107 70-71 -0.19276166536077 1107 72-73 -0.10194280144028767 1107 74-75 -0.19253123122501137 1107 76-77 -0.033937584068773674 1107 78-79 0.06012481532818725 1107 80-81 0.08674898765575634 1107 82-83 0.060615188160021205 1107 84-85 -0.02095138400614971 1107 86-87 -0.005233029475874673 1107 88-89 0.13055058488745885 1107 90-91 0.010816735065805716 1107 92-93 0.14038225214554245 1107 94-95 0.14345963058583422 1107 96-97 0.10459881976242968 1107 98-99 0.2749010542911776 1107 100-101 0.36403691634384927 1108 1 -0.034204114436583666 1108 2 -0.036589004293936966 1108 3 0.015002814562230071 1108 4 -0.03476262055448842 1108 5 0.0018571248819725383 1108 6 0.019543358584016346 1108 7 -0.008258465497938516 1108 8 -0.008612895613424598 1108 9 -0.050022758540627876 1108 10-11 0.020340127576083944 1108 12-13 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5 59.0 6 125.0 7 365.0 8 831.0 9 1575.0 10 2765.0 11 4279.0 12 6193.0 13 8783.0 14 10601.0 15 14498.0 16 18303.0 17 24711.0 18 23675.0 19 16840.0 20 15614.0 21 16420.0 22 17571.0 23 19555.0 24 22558.0 25 26558.0 26 31855.0 27 38816.0 28 47692.0 29 57729.0 30 70831.0 31 86868.0 32 107200.0 33 137395.0 34 187237.0 35 267439.0 36 421328.0 37 683430.0 38 574147.0 39 51607.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27427607698968 19.080530324895822 14.38249576705713 23.262697831057363 2 15.750436661890218 20.600194130906043 39.50928721060683 24.14008199659691 3 19.71277645995296 24.302078832269768 29.58748674759765 26.39765796017962 4 13.174206124309439 16.287662606189173 36.47992980247661 34.05820146702477 5 13.607767358615893 37.772853575089435 33.88993699533509 14.729442070959589 6 32.67554106445683 35.75298351081134 17.005721456306784 14.565753968425044 7 29.08415246472299 32.76597409841383 20.138544601857543 18.011328835005642 8 26.670383032149658 35.18355717194977 19.436403557496938 18.70965623840363 9 26.707458254725353 16.810098255972246 19.965173167487485 36.51727032181492 10-11 25.368986344180765 26.578673257522574 26.814920024632805 21.237420373663856 12-13 26.070049620443235 23.730778835022225 27.64979235554011 22.54937918899443 14-15 22.66121070485442 25.247652314754475 26.027129912711906 26.064007067679196 16-17 22.1696857889814 28.545649630149295 26.827378851115753 22.457285729753554 18-19 22.12513650347489 26.988075905383234 29.20930621248745 21.67748137865442 20-21 22.784697417435503 26.17968350759462 29.204381641331377 21.8312374336385 22-23 22.454203975405665 26.055823081547913 29.21219131478091 22.277781628265515 24-25 22.253715195304242 26.311964483354682 29.36191909268402 22.072401228657057 26-27 22.008980069511885 26.525627720256523 29.236843322675465 22.228548887556126 28-29 21.877929663859053 26.909581225744 29.183091576131382 22.029397534265563 30-31 22.18874140231226 26.46222455978148 29.090204547120184 22.258829490786077 32-33 21.94428701660857 26.54746772581977 29.048735346913652 22.459509910658 34-35 22.456884951755022 26.678973526323198 28.828097446053025 22.036044075868755 36-37 22.585485181684 26.187455428959982 29.0209463157371 22.20611307361892 38-39 22.164160282349833 26.57010582646675 28.98184953137641 22.283884359807 40-41 22.43441879795174 26.517212334352315 28.926808129699193 22.121560737996756 42-43 22.216121914330785 26.551898705631103 28.685694652708882 22.546284727329226 44-45 22.261164076048647 26.767633974798912 28.640579254041953 22.33062269511049 46-47 22.58697431394189 26.967608559543265 28.064429372625593 22.380987753889254 48-49 22.45788496798697 27.018701100417807 27.983303548244713 22.540110383350505 50-51 22.4561439579823 27.246325722743 27.947836687292384 22.34969363198232 52-53 22.79203001966015 27.130544765436998 27.745900708359056 22.331524506543797 54-55 22.252263395911314 27.105701857508446 27.95740395179425 22.68463079478599 56-57 22.378701305592124 27.364425018819492 27.719276137543154 22.537597538045226 58-59 22.504621164035903 26.93501917111539 27.818843737336152 22.741515927512555 60-61 22.319781536997784 27.120887050787605 28.23654864537584 22.322782766838763 62-63 22.26536488110556 27.105657268837003 28.222108290376752 22.406869559680683 64-65 22.009520507526055 27.075777555923324 28.096987869800948 22.817714066749673 66-67 21.968826967150623 27.292023090103733 27.82701059296811 22.912139349777533 68-69 22.176863619541326 27.017260293233967 28.026586847316974 22.77928923990773 70-71 22.259890677836545 27.030539968449585 27.79845802889148 22.911111324822393 72-73 22.22318023830945 27.076966901907518 27.776123357688682 22.923729502094353 74-75 22.04372355944687 27.49152625559154 27.544419797638263 22.920330387323325 76-77 22.071164215101742 27.083278973834478 27.761087218784112 23.08446959227966 78-79 22.230343250900102 27.152510155062643 27.666671862061605 22.950474731975657 80-81 22.263749113316127 27.27877340986367 27.507180522701596 22.950296954118606 82-83 22.241767592740285 27.243142161591592 27.600513481884043 22.914576763784073 84-85 22.14584623200666 27.279355169688785 27.45813202559716 23.1166665727074 86-87 22.152620648629124 27.116463239746324 27.472559939237495 23.258356172387053 88-89 22.06784936238133 27.09959218230722 27.371392989955318 23.461165465356128 90-91 22.02451455947177 27.082614331665418 27.489420899277643 23.403450209585174 92-93 22.187647053166465 26.914754512615026 27.460121751316784 23.43747668290172 94-95 21.97974094098456 26.790086057309747 27.564785894699384 23.665387107006307 96-97 21.95624195197399 26.542377344187067 27.72175410239991 23.779626601439034 98-99 21.850504611023545 26.70555143424404 27.388557949600912 24.055386005131503 100-101 21.808200007627295 26.267631906641885 27.99577845686515 23.92838962886567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 693.0 1 460.0 2 342.0 3 500.0 4 472.0 5 470.5 6 828.5 7 1699.0 8 2674.0 9 3084.5 10 2836.5 11 2551.0 12 2671.5 13 2953.5 14 3215.0 15 3387.0 16 3527.0 17 3826.5 18 4103.5 19 4431.5 20 5021.5 21 5832.0 22 6945.5 23 8447.0 24 10380.0 25 12764.0 26 16363.5 27 21069.5 28 25218.5 29 29462.0 30 34336.5 31 40761.5 32 48743.0 33 53876.5 34 56742.0 35 60802.0 36 67553.5 37 74496.5 38 78827.5 39 81688.0 40 85003.0 41 91508.0 42 96408.0 43 99761.0 44 104186.0 45 109156.0 46 110884.5 47 114177.5 48 122979.0 49 121639.0 50 113915.0 51 109423.5 52 106528.0 53 106806.0 54 105488.0 55 97318.5 56 86917.0 57 78247.5 58 72049.0 59 63807.0 60 52418.0 61 43212.5 62 35894.5 63 27071.0 64 19296.5 65 14629.5 66 10961.5 67 8835.5 68 6778.0 69 4606.0 70 3259.0 71 2235.0 72 1682.0 73 1363.0 74 1048.0 75 868.0 76 588.5 77 316.0 78 182.0 79 125.0 80 85.0 81 45.5 82 27.5 83 21.0 84 11.0 85 5.5 86 5.5 87 3.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2695083487233091 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0024539950542051027 16-17 5.803366682241797E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1606733364483596E-4 26-27 0.0011772543841119076 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003299628485046051 36-37 0.021256903104668524 38-39 0.007444890400933049 40-41 0.01808992300093086 42-43 0.005322516299998905 44-45 8.787955261680435E-4 46-47 9.948628598128796E-5 48-49 0.0 50-51 0.0 52-53 1.6581047663547994E-5 54-55 0.0 56-57 3.316209532709599E-5 58-59 5.637556205606317E-4 60-61 0.002006306767289307 62-63 0.0014259700990651273 64-65 1.6581047663547994E-5 66-67 0.0 68-69 4.974314299064398E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2311563854775226 78-79 0.0 80-81 0.0013928080037380315 82-83 0.0 84-85 0.0 86-87 0.10303463018128724 88-89 0.3553981756204877 90-91 0.35635987638497346 92-93 0.0 94-95 1.6581047663547994E-5 96-97 0.0 98-99 0.0 100-101 1.989725719625759E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3015491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.31869936620402 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65680607005177 33.90706863971163 2 14.651601168393697 13.865894218414898 3 5.408017574474489 7.677010733211187 4 2.5948061322875042 4.911314051491802 5 1.4528507483651953 3.43735038929286 6 0.9062088261732841 2.5728373805216562 7 0.5983633166975005 1.981964172620163 8 0.4379595674297604 1.6578941684609272 9 0.31606377513163647 1.3460154080401274 >10 1.7738057487038 15.443865512784763 >50 0.13145111923395875 4.2794951898805715 >100 0.06582214646873077 5.402586225040421 >500 0.004349618073197457 1.4675838542834754 >1k 0.0016837231249349526 1.2842396114490242 >5k 2.1046539061686908E-4 0.7648804447964889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9818 0.3255854519214284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7822 0.2593939096485448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5401 0.1791084768616454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3898 0.12926584758502016 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6581047663547994E-5 9.948628598128797E-5 14-15 0.0 0.0 0.0 3.316209532709599E-5 1.8239152429902792E-4 16-17 0.0 0.0 0.0 3.316209532709599E-5 2.984588579438639E-4 18-19 0.0 0.0 0.0 3.316209532709599E-5 3.6478304859805584E-4 20-21 0.0 0.0 0.0 1.989725719625759E-4 5.305935252335358E-4 22-23 0.0 0.0 0.0 4.476882869157958E-4 5.471745728970838E-4 24-25 0.0 0.0 0.0 7.958902878503036E-4 5.637556205606317E-4 26-27 0.0 0.0 0.0 0.0011275112411212635 5.803366682241797E-4 28-29 0.0 0.0 0.0 0.0027856160074760626 6.300798112148237E-4 30-31 0.0 0.0 0.0 0.007958902878503036 6.466608588783717E-4 32-33 0.0 0.0 0.0 0.01939982576635115 6.632419065419197E-4 34-35 0.0 0.0 0.0 0.036561210098123326 6.964040018690157E-4 36-37 0.0 0.0 0.0 0.06758435027662162 6.964040018690157E-4 38-39 0.0 0.0 0.0 0.11505588973735952 7.295660971961117E-4 40-41 0.0 3.316209532709599E-5 0.0 0.16765097292613373 7.461471448596596E-4 42-43 0.0 3.316209532709599E-5 0.0 0.22473952003172948 7.627281925232076E-4 44-45 0.0 3.316209532709599E-5 0.0 0.2931860847868556 7.793092401867556E-4 46-47 0.0 3.316209532709599E-5 0.0 0.37577628319898815 8.124713355138517E-4 48-49 0.0 3.316209532709599E-5 0.0 0.46746947677840855 8.622144785044956E-4 50-51 0.0 3.316209532709599E-5 0.0 0.5682822465727804 8.622144785044956E-4 52-53 0.0 3.316209532709599E-5 0.0 0.6712837146587405 8.953765738315916E-4 54-55 0.0 3.316209532709599E-5 0.0 0.7886609510689967 8.953765738315916E-4 56-57 0.0 3.316209532709599E-5 0.0 0.9305118138306498 9.119576214951396E-4 58-59 0.0 3.316209532709599E-5 0.0 1.085859649390431 9.451197168222356E-4 60-61 0.0 3.316209532709599E-5 0.0 1.2583522882343208 9.617007644857836E-4 62-63 0.0 3.316209532709599E-5 0.0 1.433680286228677 9.948628598128795E-4 64-65 0.0 3.316209532709599E-5 0.0 1.6202170724435923 0.0010114439074764277 66-67 0.0 3.316209532709599E-5 0.0 1.8385894701725192 0.0010611870504670714 68-69 0.0 3.316209532709599E-5 0.0 2.0875373197930287 0.0010943491457941674 70-71 0.0 3.316209532709599E-5 0.0 2.3653361923481118 0.0011772543841119076 72-73 3.316209532709599E-5 3.316209532709599E-5 0.0 2.6492700525387076 0.0013762269560744834 74-75 3.316209532709599E-5 3.316209532709599E-5 0.0 2.944876970284441 0.0014922942897193192 76-77 3.316209532709599E-5 3.316209532709599E-5 0.0 3.271589933446991 0.0015254563850464152 78-79 3.316209532709599E-5 3.316209532709599E-5 0.0 3.6409990943431767 0.0015917805757006073 80-81 3.316209532709599E-5 3.316209532709599E-5 0.0 4.048428597531878 0.0015917805757006073 82-83 3.316209532709599E-5 3.316209532709599E-5 0.0 4.4653590410317925 0.0017907531476631833 84-85 3.316209532709599E-5 3.316209532709599E-5 0.0 4.8886897689298365 0.0018570773383173751 86-87 3.316209532709599E-5 3.316209532709599E-5 0.0 5.3442043103428265 0.001923401528971567 88-89 3.316209532709599E-5 3.316209532709599E-5 0.0 5.836943303760481 0.002006306767289307 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGT 90 2.4305216E-5 31.635567 6 GTATCAA 15555 0.0 28.778967 1 GGTATCA 10980 0.0 28.118877 1 GTACATG 13650 0.0 25.162868 1 TACATGG 13800 0.0 24.311243 2 ACATGGG 13810 0.0 23.709494 3 ATCAACG 19040 0.0 23.427599 3 TATCAAC 19120 0.0 23.379211 2 TCAACGC 19195 0.0 23.287865 4 CAACGCA 19295 0.0 23.117983 5 AACGCAG 19880 0.0 22.50931 6 GAGTACT 10680 0.0 21.195457 12-13 CATGGGG 6640 0.0 20.939505 4 TAAGGTG 1420 0.0 20.38489 5 ACGCAGA 22180 0.0 20.025398 7 GTACTTT 11290 0.0 19.840094 14-15 CGCAGAG 22410 0.0 19.735172 8 AGTACTT 11330 0.0 19.204592 12-13 AGAGTAC 18305 0.0 19.158733 10-11 CATGGGA 6745 0.0 18.99541 4 >>END_MODULE