##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963995 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11630375841079 33.0 31.0 34.0 30.0 34.0 2 32.15467452819381 34.0 31.0 34.0 30.0 34.0 3 32.207417534158694 34.0 31.0 34.0 30.0 34.0 4 35.67000883403471 37.0 35.0 37.0 35.0 37.0 5 35.726625067782756 37.0 35.0 37.0 35.0 37.0 6 35.84987690905527 37.0 36.0 37.0 35.0 37.0 7 35.80727140344044 37.0 36.0 37.0 35.0 37.0 8 35.803099295059305 37.0 36.0 37.0 35.0 37.0 9 37.441969047782706 39.0 37.0 39.0 35.0 39.0 10-11 37.35547901089361 39.0 37.0 39.0 34.5 39.0 12-13 37.29579377747906 39.0 37.0 39.0 34.0 39.0 14-15 38.501807031076964 40.0 38.0 41.0 34.0 41.0 16-17 38.47500451885061 40.0 38.0 41.0 34.0 41.0 18-19 38.48536885277203 40.0 38.0 41.0 34.0 41.0 20-21 38.481956675042454 40.0 38.0 41.0 34.0 41.0 22-23 38.47416974075799 40.0 38.0 41.0 34.0 41.0 24-25 38.3808619675712 40.0 38.0 41.0 34.0 41.0 26-27 38.2768489736481 40.0 38.0 41.0 34.0 41.0 28-29 38.156627435405895 40.0 38.0 41.0 34.0 41.0 30-31 37.985977051876404 40.0 38.0 41.0 33.5 41.0 32-33 37.74885017528049 40.0 37.0 41.0 33.0 41.0 34-35 37.44260703311363 40.0 37.0 41.0 32.5 41.0 36-37 37.17713207009183 40.0 36.5 41.0 31.5 41.0 38-39 36.61027293857673 39.5 35.5 40.5 30.5 41.0 40-41 36.623651791374215 39.0 36.0 40.5 31.0 41.0 42-43 36.73468415143623 40.0 36.0 41.0 31.0 41.0 44-45 36.56135606251543 40.0 36.0 41.0 30.0 41.0 46-47 36.1996303452911 40.0 36.0 41.0 29.5 41.0 48-49 35.87464810246462 40.0 36.0 41.0 28.0 41.0 50-51 34.69857509820544 38.0 34.0 40.0 25.5 40.5 52-53 34.829647987902206 38.5 34.5 39.5 26.0 40.5 54-55 35.35370380270825 39.0 35.0 40.5 27.0 41.0 56-57 35.25892275693167 39.0 35.0 41.0 26.0 41.0 58-59 35.077245868752215 39.0 35.0 41.0 26.0 41.0 60-61 34.73839877392763 38.5 35.0 40.0 25.5 41.0 62-63 34.40484751743258 38.0 34.5 40.0 24.5 41.0 64-65 34.02454665108618 37.5 34.0 40.0 23.0 41.0 66-67 33.61670752725949 37.0 34.0 39.5 22.0 41.0 68-69 33.18704019103918 36.0 33.5 39.0 21.5 41.0 70-71 32.735704011466424 36.0 33.0 39.0 20.0 40.0 72-73 32.28604451640661 35.0 32.5 38.0 18.5 40.0 74-75 31.839644703779797 35.0 32.0 37.0 16.5 39.5 76-77 31.402578163386366 35.0 32.0 37.0 12.0 39.0 78-79 31.011011993411387 35.0 31.0 36.0 7.5 39.0 80-81 30.590111736537004 35.0 31.0 36.0 4.5 37.5 82-83 30.163512381650666 34.5 31.0 35.0 2.0 37.0 84-85 29.751482564874145 34.0 30.5 35.0 2.0 36.5 86-87 29.408514532878137 34.0 30.0 35.0 2.0 36.0 88-89 28.96481890228845 34.0 29.5 35.0 2.0 36.0 90-91 28.544533209096763 34.0 29.0 35.0 2.0 35.5 92-93 28.128771967342075 34.0 29.0 35.0 2.0 35.0 94-95 27.6789872173809 34.0 28.0 35.0 2.0 35.0 96-97 27.097902234985323 34.0 26.5 35.0 2.0 35.0 98-99 26.413712356701517 33.5 25.0 35.0 2.0 35.0 100-101 23.80755755488176 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18985898012579128 1101 2 0.20116005928377945 1101 3 0.2591846491977776 1101 4 0.18147034994106548 1101 5 0.19923080399667725 1101 6 0.21502231395778892 1101 7 0.20288232703872922 1101 8 0.026066359658834415 1101 9 0.2277094268880333 1101 10-11 0.26551760643527444 1101 12-13 0.3088773755444336 1101 14-15 0.36133832015394773 1101 16-17 0.30733209915162263 1101 18-19 0.3860194782120985 1101 20-21 0.4324353515109891 1101 22-23 0.38352065814029146 1101 24-25 0.4089490927522803 1101 26-27 0.35020656163167985 1101 28-29 0.4037320337768975 1101 30-31 0.4194641788341329 1101 32-33 0.28696411316102655 1101 34-35 0.2739574080962299 1101 36-37 0.28233852763975875 1101 38-39 0.5729694249843291 1101 40-41 0.35099953869549694 1101 42-43 0.3035756368556619 1101 44-45 0.2798317927888476 1101 46-47 0.34612208369514263 1101 48-49 0.3408147985071679 1101 50-51 0.32226187938618267 1101 52-53 0.366310173245445 1101 54-55 0.24545044488917966 1101 56-57 0.2717258976400032 1101 58-59 0.2287953182350222 1101 60-61 0.33863255571177575 1101 62-63 0.3734171376354283 1101 64-65 0.2992572239704714 1101 66-67 0.27699253393362255 1101 68-69 0.41883412214573923 1101 70-71 0.3871770513529924 1101 72-73 0.32154223189278497 1101 74-75 0.26336514553680246 1101 76-77 0.24002687747173468 1101 78-79 0.11626046027767245 1101 80-81 0.11591702196812648 1101 82-83 0.25134921532177046 1101 84-85 0.23242128732420753 1101 86-87 0.2549079856998837 1101 88-89 0.19718288820130425 1101 90-91 0.20281189877040973 1101 92-93 0.1478765701500535 1101 94-95 0.21147703343131852 1101 96-97 0.2026909836701094 1101 98-99 0.09220088954146277 1101 100-101 -0.23796740807128458 1102 1 0.2867999335118441 1102 2 0.20532787891135484 1102 3 0.3053760171135025 1102 4 0.1218992728503423 1102 5 0.16964100067571763 1102 6 0.19973351601874612 1102 7 0.25489212559768504 1102 8 0.2453445096016651 1102 9 0.2766507406414078 1102 10-11 0.37830185151937457 1102 12-13 0.30653391546047715 1102 14-15 0.39873179192839103 1102 16-17 0.34610000758400616 1102 18-19 0.4413814435867991 1102 20-21 0.37708196631050583 1102 22-23 0.3865532134663283 1102 24-25 0.329912281659837 1102 26-27 0.3476228566695454 1102 28-29 0.43036703960069644 1102 30-31 0.31865624817909577 1102 32-33 0.23975170451306838 1102 34-35 0.22384221925732106 1102 36-37 0.167775505187592 1102 38-39 0.6312416782024286 1102 40-41 0.4524767229397284 1102 42-43 0.284936817144434 1102 44-45 0.32146387062818604 1102 46-47 0.5362088721573883 1102 48-49 0.46074364741244267 1102 50-51 0.32933408798738384 1102 52-53 0.37558266027329523 1102 54-55 0.4596818436077328 1102 56-57 0.4144962428418637 1102 58-59 0.4948445341143568 1102 60-61 0.4539736927135607 1102 62-63 0.5010751147306465 1102 64-65 0.5636939033572475 1102 66-67 0.5037831626673324 1102 68-69 0.48113262211677466 1102 70-71 0.5161853333661597 1102 72-73 0.3991424000641999 1102 74-75 0.4069865367691534 1102 76-77 0.3708595190285209 1102 78-79 0.4105475657773461 1102 80-81 0.55909589071506 1102 82-83 0.4203389641586206 1102 84-85 0.3513961281083482 1102 86-87 0.430988176008146 1102 88-89 0.3221751124184209 1102 90-91 0.3365457117354822 1102 92-93 0.2089674105995698 1102 94-95 0.20069711704512017 1102 96-97 0.3259564478253587 1102 98-99 0.44462403799349204 1102 100-101 0.3925316792126843 1103 1 0.2686324253535801 1103 2 0.356090198752824 1103 3 0.3732926332284592 1103 4 0.2730597846514442 1103 5 0.2720976584141752 1103 6 0.20821687128374577 1103 7 0.21494227289760204 1103 8 0.25018814148564417 1103 9 0.3820347314581056 1103 10-11 0.2825957918465676 1103 12-13 0.3048104908851883 1103 14-15 0.4632855525092552 1103 16-17 0.5200538981994285 1103 18-19 0.4869759784021497 1103 20-21 0.4340529698475706 1103 22-23 0.4720664922349158 1103 24-25 0.5642420281546592 1103 26-27 0.575285998715195 1103 28-29 0.36481468824606367 1103 30-31 0.36610797518117977 1103 32-33 0.4383219465080117 1103 34-35 0.3822401810122287 1103 36-37 0.07795210954343901 1103 38-39 0.6032920245983249 1103 40-41 0.42549766223722685 1103 42-43 0.5299104213727261 1103 44-45 0.5286699007476798 1103 46-47 0.4391915788050156 1103 48-49 0.3467954185889397 1103 50-51 0.31196918549775177 1103 52-53 0.49865508454682583 1103 54-55 0.5289190260263155 1103 56-57 0.4709586183679946 1103 58-59 0.5778317826390165 1103 60-61 0.46991848090965505 1103 62-63 0.531700120563535 1103 64-65 0.3522275303571547 1103 66-67 0.4939030246689313 1103 68-69 0.49771047331119433 1103 70-71 0.383960715770435 1103 72-73 0.45579142807140016 1103 74-75 0.28697365774796424 1103 76-77 0.2919786250875731 1103 78-79 0.4198770789565671 1103 80-81 0.4571478609875399 1103 82-83 0.41974323165651484 1103 84-85 0.41437578609878756 1103 86-87 0.3196064187994736 1103 88-89 0.35149821719578966 1103 90-91 0.17658453545154984 1103 92-93 0.23010891495634667 1103 94-95 0.2699654082105418 1103 96-97 0.34630934544958336 1103 98-99 0.2545822589464777 1103 100-101 -0.12253739775968953 1104 1 0.23591977644242945 1104 2 0.3107503904719735 1104 3 0.2987326008537181 1104 4 0.2558040495172662 1104 5 0.19494157676913204 1104 6 0.14940310190316808 1104 7 0.23569245627013657 1104 8 0.21628976781035192 1104 9 0.32052374175226106 1104 10-11 0.2979510287133067 1104 12-13 0.3373804886088365 1104 14-15 0.33832361811868594 1104 16-17 0.299644661282116 1104 18-19 0.34255429744507637 1104 20-21 0.3663604286835991 1104 22-23 0.35355623601921593 1104 24-25 0.4384813482851655 1104 26-27 0.4000354738903411 1104 28-29 0.40574950879391025 1104 30-31 0.3800559226733995 1104 32-33 0.40587689503723823 1104 34-35 0.24756228435845884 1104 36-37 0.30672768685077045 1104 38-39 0.6397616606354504 1104 40-41 0.4141058014485992 1104 42-43 0.27771627411825506 1104 44-45 0.132991422360341 1104 46-47 0.22568625467824432 1104 48-49 0.24285581770875808 1104 50-51 0.19049815835861494 1104 52-53 0.23837787585623005 1104 54-55 0.22438049250075665 1104 56-57 0.1200432732482355 1104 58-59 0.13707502314790787 1104 60-61 0.17652090395642261 1104 62-63 0.2198201094347283 1104 64-65 0.1431069597524015 1104 66-67 0.2532626118910315 1104 68-69 0.2324834707566268 1104 70-71 0.29376419099616413 1104 72-73 0.24200788354349356 1104 74-75 0.2301155248597766 1104 76-77 0.2696349975993968 1104 78-79 0.15153906646194315 1104 80-81 0.039169258665662454 1104 82-83 0.20423573235206405 1104 84-85 0.09485837203095215 1104 86-87 0.24101168475288404 1104 88-89 0.24418911433011914 1104 90-91 0.19279567944325038 1104 92-93 0.16929549967446178 1104 94-95 0.38746091874498134 1104 96-97 0.2536285654207511 1104 98-99 0.3857571078222115 1104 100-101 0.26022963059349635 1105 1 0.25631010615455807 1105 2 0.369282414782397 1105 3 0.27471726024627685 1105 4 0.2343978799940487 1105 5 0.14157401232828448 1105 6 0.25834328462754996 1105 7 0.2916096043117591 1105 8 0.18310080734252665 1105 9 0.3538125429503509 1105 10-11 0.3921872075074546 1105 12-13 0.31712660789474256 1105 14-15 0.3240904503941806 1105 16-17 0.3069453958246271 1105 18-19 0.2454037201913266 1105 20-21 0.3401346460035839 1105 22-23 0.29042614988678395 1105 24-25 0.21632066810037998 1105 26-27 0.31815212906739987 1105 28-29 0.3037961169824328 1105 30-31 0.37281683581989 1105 32-33 0.38971172994346404 1105 34-35 0.21711070315070202 1105 36-37 0.2008941356110867 1105 38-39 0.6155130413693612 1105 40-41 0.4733739530480321 1105 42-43 0.33318730842068334 1105 44-45 0.27488903014474886 1105 46-47 0.41197859784509916 1105 48-49 0.41299377224160594 1105 50-51 0.3421197778386471 1105 52-53 0.2788992663364951 1105 54-55 0.38847919219410443 1105 56-57 0.34819693288643094 1105 58-59 0.34058054212160727 1105 60-61 0.3627387627689558 1105 62-63 0.23926052591320257 1105 64-65 0.38114786275633605 1105 66-67 0.403004690004515 1105 68-69 0.3805404592115522 1105 70-71 0.36921763431278265 1105 72-73 0.37565405116499306 1105 74-75 0.2786056319112262 1105 76-77 0.2795216070557416 1105 78-79 0.30568554707620166 1105 80-81 0.28825413121025534 1105 82-83 0.0412533471094747 1105 84-85 0.15745676665540032 1105 86-87 0.2986158241812582 1105 88-89 0.32735913675466577 1105 90-91 0.2188721764599144 1105 92-93 0.14396479706513077 1105 94-95 0.10938340747327757 1105 96-97 0.09141796487083198 1105 98-99 0.1488257047430004 1105 100-101 -0.05008946354996979 1106 1 0.14868332941308893 1106 2 0.1819611146368061 1106 3 0.20781078606780312 1106 4 0.16847965298796197 1106 5 0.21887046061432613 1106 6 0.1576051020450251 1106 7 0.13113657760262498 1106 8 0.180316530937759 1106 9 0.09757081364860909 1106 10-11 0.29959180701909105 1106 12-13 0.35139282990964205 1106 14-15 0.37074246579109627 1106 16-17 0.4327136732687791 1106 18-19 0.3678634210235785 1106 20-21 0.43220812473769143 1106 22-23 0.4203515170084131 1106 24-25 0.33898729433335006 1106 26-27 0.4370896384883096 1106 28-29 0.4405359002637468 1106 30-31 0.39476713150617115 1106 32-33 0.44030439580561165 1106 34-35 0.39834271795895404 1106 36-37 0.46960921509154474 1106 38-39 0.989209612026805 1106 40-41 0.7237471471705206 1106 42-43 0.7171327811991404 1106 44-45 0.592341730892862 1106 46-47 0.5330286193849645 1106 48-49 0.5360742492760124 1106 50-51 0.5209140270963459 1106 52-53 0.5453849922001837 1106 54-55 0.537848114317903 1106 56-57 0.5269241395035209 1106 58-59 0.5769572843011801 1106 60-61 0.5655049926624756 1106 62-63 0.4243807165699778 1106 64-65 0.535120577739491 1106 66-67 0.48487951143472685 1106 68-69 0.4494061839514174 1106 70-71 0.3680389622565414 1106 72-73 0.47371840674845345 1106 74-75 0.6898526220534364 1106 76-77 0.7075005672615475 1106 78-79 0.7699796178012832 1106 80-81 0.7642377032362013 1106 82-83 0.6823812273044254 1106 84-85 0.6164688881432028 1106 86-87 0.8049145620255054 1106 88-89 0.8073633347240019 1106 90-91 0.8254613531316899 1106 92-93 0.718927388460898 1106 94-95 0.7751829794112233 1106 96-97 0.8830211937395411 1106 98-99 1.1268129746685638 1106 100-101 0.7790191428520501 1107 1 0.1201161182091468 1107 2 0.02162334712937053 1107 3 0.11747053297970211 1107 4 -0.03533796138056289 1107 5 0.09674446627602151 1107 6 0.04626805476215168 1107 7 0.09377651876307169 1107 8 0.08422890276705175 1107 9 0.11286180049199857 1107 10-11 0.10063252727758965 1107 12-13 0.18038530445364387 1107 14-15 0.23238724153318202 1107 16-17 0.23352152940031345 1107 18-19 0.3320973574283457 1107 20-21 0.23921805603216484 1107 22-23 0.19378956709159212 1107 24-25 0.2642608142464127 1107 26-27 0.3595068614415027 1107 28-29 0.4344076290317602 1107 30-31 0.2870893170526472 1107 32-33 0.3569895134550194 1107 34-35 0.29105004126004275 1107 36-37 0.230675775209086 1107 38-39 0.536440310390141 1107 40-41 0.4454494899787491 1107 42-43 0.29606396454762063 1107 44-45 0.2678572343841239 1107 46-47 0.3538627904353291 1107 48-49 0.3930473210210579 1107 50-51 0.255663547582877 1107 52-53 0.2915524897973114 1107 54-55 0.2435914174594913 1107 56-57 0.15364973055000064 1107 58-59 0.18293633586128522 1107 60-61 0.14996638090593706 1107 62-63 0.24763217843922547 1107 64-65 0.14530009095591367 1107 66-67 0.188359494027182 1107 68-69 0.2597678316962799 1107 70-71 0.2058399783754865 1107 72-73 0.3565169160787107 1107 74-75 0.33781635463209625 1107 76-77 0.3291841621746734 1107 78-79 0.43624502861347025 1107 80-81 0.291289813053055 1107 82-83 0.29051034855410407 1107 84-85 0.26762782193938506 1107 86-87 0.40068746308836367 1107 88-89 0.45566936484448917 1107 90-91 0.4622234304663344 1107 92-93 0.41642882848587703 1107 94-95 0.5201534960132896 1107 96-97 0.4387546139409544 1107 98-99 0.7199514147249246 1107 100-101 0.5965401960595962 1108 1 0.1609737894769836 1108 2 0.11812324408623454 1108 3 0.2023415048206303 1108 4 0.17291889045409192 1108 5 0.21172966116635905 1108 6 0.16493237012326745 1108 7 0.3099834421940173 1108 8 0.26552822866206327 1108 9 0.39389486933015405 1108 10-11 0.37621338624090583 1108 12-13 0.34527621220494353 1108 14-15 0.44749259112705175 1108 16-17 0.44723059110980046 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-0.2360383127822594 2316 82-83 -0.42651328566229196 2316 84-85 -0.33531533784308465 2316 86-87 -0.3077269716198643 2316 88-89 -0.5134981401734677 2316 90-91 -0.509284775656301 2316 92-93 -0.48369346145245373 2316 94-95 -0.6698961854132186 2316 96-97 -0.6619538536500862 2316 98-99 -0.7520801534536901 2316 100-101 -0.11829400478507424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6886.0 3 1167.0 4 1104.0 5 1240.0 6 1428.0 7 1718.0 8 2210.0 9 2798.0 10 3382.0 11 4753.0 12 6923.0 13 10287.0 14 10744.0 15 11925.0 16 16688.0 17 23138.0 18 19263.0 19 10722.0 20 10171.0 21 10350.0 22 11156.0 23 12371.0 24 14363.0 25 16189.0 26 18820.0 27 22027.0 28 26795.0 29 32051.0 30 39217.0 31 48388.0 32 60437.0 33 79844.0 34 109706.0 35 164135.0 36 255103.0 37 417418.0 38 416583.0 39 62494.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.379975498169 20.39177396218302 14.496933696452036 20.73131684319594 2 14.629809205455471 22.73843706912075 39.52639818484264 23.105355540581144 3 20.55663726248853 27.048851268045617 30.76491841628583 21.629593053180024 4 11.718795441117495 17.082441730775315 40.1800756548368 31.018687173270393 5 11.831253565644081 40.094168723981646 34.308104307144966 13.766473403229313 6 28.13198979706268 38.470364789671464 18.043386951087477 15.35425846217838 7 26.55014968228202 35.176661799613356 21.732072793715794 16.54111572438883 8 25.41799215896628 32.70952126251962 20.694149206030325 21.178337372483774 9 26.334593970865456 18.50001300239601 22.923989012720423 32.24140401401811 10-11 24.356339229154425 26.895701705526744 27.629660691821094 21.118298373497737 12-13 26.846889282609176 25.56484290229118 27.457147233970534 20.131120581129107 14-15 23.172056716615767 26.43532704848241 28.520315296253052 21.872300938648774 16-17 20.098608169517355 29.98190441965435 28.78881948001968 21.130667930808617 18-19 19.812231564074974 29.489653386075325 31.128458930817178 19.569656119032523 20-21 21.583553872054733 27.215336744649566 30.18316146698498 21.01794791631072 22-23 20.794337230308475 27.341780803053656 30.525596914517823 21.338285052120046 24-25 21.673551199857567 27.765304503489713 30.441956931747978 20.119187364904747 26-27 21.207660612144085 26.6160036172591 32.3317524401325 19.844583330464314 28-29 19.679426759778927 28.670860186754965 31.629173883683116 20.02053916978299 30-31 20.1910557765775 27.503045886943404 31.05789811616546 21.24800022031363 32-33 20.405912340071765 27.887761425449277 31.027952536027687 20.678373698451274 34-35 21.788797513840173 27.161868390804745 30.727113352097735 20.322220743257347 36-37 21.51477783055559 26.347017281877775 31.58831463387246 20.54989025369417 38-39 20.599496775579297 27.963475316944447 31.294601077023 20.14242683045326 40-41 20.31384590996777 28.112565214111946 31.269944556019897 20.30364431990039 42-43 19.924511490784255 28.374273450590547 29.783666780554967 21.917548278070228 44-45 20.624520632832436 27.363831592160103 29.96227976603174 22.049368008975723 46-47 21.866368215801256 28.405501016888117 29.27144160770625 20.456689159604373 48-49 21.285463943078128 28.0827287588512 29.407604401484694 21.224202896585982 50-51 19.697903648983846 28.50868061505786 30.664651872525734 21.12876386343256 52-53 20.747131758279366 29.607155628107463 28.645788837453427 20.99992377615974 54-55 19.921768407377144 28.228152887906838 29.41014299834918 22.439935706366835 56-57 21.23974527112877 28.263860660955466 28.708434634332153 21.787959433583616 58-59 22.270980678052407 27.41355634468874 28.8079482048697 21.507514772389154 60-61 21.09576412668936 28.175167524238827 30.2600413617384 20.469026987333415 62-63 20.697279986003856 28.110733214158103 30.056227553931315 21.13575924590673 64-65 20.186848143552737 29.1954700349055 29.359830513806816 21.257851307734942 66-67 20.75622328838466 28.177358475121682 28.41258951620577 22.653828720287883 68-69 20.157736018190118 28.17119543527957 29.231074645376644 22.439993901153667 70-71 21.358757880153117 28.246096120217295 28.776206209576007 21.618939790053588 72-73 21.297614559123932 27.025097545291356 29.981741751942593 21.695546143642126 74-75 20.09197071180225 29.540698971270473 28.83919015431966 21.528140162607613 76-77 20.585482297736828 27.989365190024596 29.00456194065846 22.420590571580117 78-79 20.683668015966575 27.86865166326998 29.390309077472658 22.057371243290792 80-81 21.23293293147398 27.962798604180556 28.937095305563716 21.86717315878175 82-83 20.901380572252762 27.527513627349066 29.64577203743413 21.92533376296404 84-85 20.366159701360086 27.904633475872835 29.30106200072067 22.428144822046406 86-87 20.434072874179172 28.140444307595047 29.303397073156262 22.122085745069523 88-89 20.49707820706673 27.752045561964312 28.650808915151377 23.10006731581758 90-91 20.59127257490734 26.993732578237896 29.361979483089268 23.053015363765496 92-93 21.453345264535525 27.69284726475143 29.33566431777519 21.518143152937856 94-95 20.934386218120487 26.967744510099813 29.528133220332997 22.5697360514467 96-97 20.254482451475226 26.828736088226997 30.186926239688077 22.729855220609696 98-99 20.7862366255923 27.418571766225025 28.54932760891119 23.24586399927149 100-101 20.517319163867864 26.280305595595298 29.85991830187386 23.342456938662977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3723.0 1 2240.0 2 963.5 3 1169.5 4 1010.0 5 1015.5 6 1730.0 7 3298.0 8 5015.5 9 5324.5 10 4341.0 11 3711.5 12 3756.0 13 3882.5 14 3962.0 15 3916.0 16 3889.0 17 4002.5 18 4161.5 19 4381.0 20 4772.5 21 5334.5 22 5977.5 23 6777.0 24 7640.0 25 9309.0 26 12541.0 27 16048.0 28 17637.0 29 19519.0 30 24085.5 31 28781.5 32 35610.5 33 43729.0 34 51961.5 35 57952.5 36 64319.0 37 72393.5 38 72184.0 39 68526.5 40 65269.5 41 64021.5 42 63735.0 43 64514.0 44 66825.0 45 66713.5 46 66910.0 47 81779.5 48 123403.5 49 119221.0 50 72376.0 51 50723.5 52 42720.5 53 40434.0 54 39157.5 55 36426.5 56 32554.0 57 28492.0 58 25500.0 59 22967.0 60 20447.0 61 18096.5 62 15440.0 63 11905.5 64 8535.0 65 6430.0 66 4771.0 67 3780.0 68 2884.0 69 1963.5 70 1581.5 71 1287.5 72 1010.5 73 884.0 74 734.0 75 574.0 76 397.0 77 230.5 78 168.0 79 129.0 80 75.0 81 53.5 82 39.0 83 22.5 84 16.5 85 15.0 86 11.0 87 5.0 88 5.5 89 7.0 90 3.5 91 3.5 92 5.0 93 3.0 94 2.0 95 1.5 96 1.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29399260181415937 2 0.1840126884233412 3 0.18411452167648085 4 0.2057540879686557 5 0.22001074340820623 6 0.11237299483959991 7 0.12764798281054687 8 0.14124272210468966 9 0.1434830536737619 10-11 0.13123760498371942 12-13 0.12044328015091688 14-15 0.13569280980857892 16-17 0.1115074121879129 18-19 0.139995264753729 20-21 0.16629370237704272 22-23 0.16950144985094157 24-25 0.1936868474716076 26-27 0.172836488891265 28-29 0.17062161563547767 30-31 0.1605910402012225 32-33 0.17057069900890787 34-35 0.35236851417646176 36-37 0.4714625037232783 38-39 0.438137571633329 40-41 0.42859070415148715 42-43 0.45127406128834346 44-45 0.4553728497272142 46-47 0.4588097220206772 48-49 0.47074967095130077 50-51 0.4644614675699276 52-53 0.4697567967331892 54-55 0.4532852680378514 56-57 0.4170071716068524 58-59 0.4063655966537593 60-61 0.3622208814177226 62-63 0.46693092395856406 64-65 0.46565800829431847 66-67 0.4745938762573224 68-69 0.4850827013307061 70-71 0.47866720638290833 72-73 0.4715897952897029 74-75 0.47612137505441715 76-77 0.45346347623084576 78-79 0.4409379860946693 80-81 0.43709378078864763 82-83 0.4494665210451147 84-85 0.4513504362281981 86-87 0.4524196853861644 88-89 0.4598280545520737 90-91 0.4767578328865399 92-93 0.4814676208442486 94-95 0.48006741361357846 96-97 0.4786417480696234 98-99 0.475001209269881 100-101 0.45789322274242045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1963995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.865320574040798 #Duplication Level Percentage of deduplicated Percentage of total 1 68.49306741674943 19.77074348083861 2 13.642723431804086 7.87603170724006 3 5.888205654795012 5.098948314946136 4 3.099227287762259 3.578407567722904 5 1.9503109024805447 2.8148174709573874 6 1.2939567497295954 2.241028583393319 7 0.9194690330637627 1.857853787810218 8 0.6457033263873662 1.4910746809516657 9 0.5076670051869784 1.318857376462683 >10 2.8867067791777745 16.04862581188738 >50 0.35920526755928056 7.3077020206897725 >100 0.2841875504895959 15.984551255057076 >500 0.019831105504067765 3.896825514578123 >1k 0.007436664564025412 4.2424308622720694 >5k 0.0015935709780054452 3.1592070026113914 >10k+ 7.082537680024202E-4 3.3128945625811785 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26182 1.3330991168511122 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14331 0.7296861753721369 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12899 0.6567735661241499 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11407 0.5808059592819738 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9815 0.4997466897828151 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8685 0.44221090175891486 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7382 0.37586653733843517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6656 0.3389010664487435 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6309 0.32123299702901487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6175 0.31441016906865854 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5982 0.30458326014068265 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5496 0.27983777962774853 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5312 0.2704691203389011 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4756 0.24215947596607934 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4593 0.23386006583519817 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3786 0.1927703481933508 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3709 0.1888497679474744 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3323 0.16919595009152263 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3177 0.16176212261232845 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3175 0.1616602893591888 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2996 0.15254621320319042 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2923 0.14882929946359333 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2642 0.134521727397473 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2490 0.12678240015885986 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2409 0.12265815340670419 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2391 0.12174165412844737 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2244 0.11425691002268336 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2193 0.11166016206762237 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1981 0.10086583723481984 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5274987970946972E-4 2 0.0 0.0 0.0 0.0 1.5274987970946972E-4 3 0.0 0.0 0.0 0.0 1.5274987970946972E-4 4 0.0 0.0 0.0 0.0 1.5274987970946972E-4 5 0.0 0.0 0.0 0.0 2.545831328491162E-4 6 0.0 0.0 0.0 0.0 2.545831328491162E-4 7 0.0 0.0 0.0 0.0 2.545831328491162E-4 8 0.0 0.0 0.0 0.0 2.545831328491162E-4 9 0.0 0.0 0.0 0.0 2.545831328491162E-4 10-11 0.0 0.0 0.0 0.0 2.545831328491162E-4 12-13 0.0 0.0 0.0 0.0 8.146660251171719E-4 14-15 0.0 0.0 0.0 2.5458313284911623E-5 9.164992782568184E-4 16-17 0.0 0.0 0.0 1.5274987970946975E-4 9.164992782568184E-4 18-19 0.0 0.0 0.0 2.545831328491162E-4 9.674159048266416E-4 20-21 0.0 0.0 0.0 4.3279132584349755E-4 0.0012729156642455812 22-23 0.0 0.0 0.0 0.001145624097821023 0.0013747489173852275 24-25 0.0 0.0 0.0 0.0026476645816308087 0.0013747489173852275 26-27 0.0 0.0 0.0 0.005168037596837059 0.0013747489173852275 28-29 0.0 0.0 0.0 0.01193994893062355 0.0013747489173852275 30-31 0.0 0.0 0.0 0.029913518109771154 0.0013747489173852275 32-33 0.0 0.0 0.0 0.06654803092675898 0.001476582170524874 34-35 0.0 0.0 0.0 0.11695549123088399 0.0015274987970946973 36-37 0.0 0.0 0.0 0.1982438855496068 0.0015274987970946973 38-39 0.0 0.0 0.0 0.3202146644976184 0.0015274987970946973 40-41 0.0 0.0 0.0 0.4496192709248241 0.0015274987970946973 42-43 0.0 0.0 0.0 0.5849556643474143 0.0015784154236645207 44-45 0.0 0.0 0.0 0.7503074091329153 0.0017311653033739902 46-47 0.0 0.0 0.0 0.9535156657730799 0.00188391518308346 48-49 0.0 0.0 0.0 1.1604917527794112 0.0019857484362231065 50-51 0.0 0.0 0.0 1.3889291978849232 0.0020366650627929297 52-53 0.0 0.0 0.0 1.6160682690129047 0.002138498315932576 54-55 0.0 0.0 0.0 1.8603662432949166 0.002138498315932576 56-57 0.0 0.0 0.0 2.17485278730343 0.002163956629217488 58-59 0.0 0.0 0.0 2.4997517814454717 0.0021894149425023992 60-61 0.0 0.0 0.0 2.867038867206892 0.002240331569072223 62-63 0.0 0.0 0.0 3.2297179982637427 0.0023930814487816924 64-65 0.0 0.0 0.0 3.5962413346266153 0.0024439980753515156 66-67 0.0 0.0 0.0 4.020656875399378 0.0025203730152062506 68-69 0.0 0.0 0.0 4.513555278908552 0.0025967479550609855 70-71 0.0 0.0 0.0 5.057370308987548 0.0025967479550609855 72-73 0.0 0.0 0.0 5.580665938558907 0.0027240395214855433 74-75 0.0 0.0 0.0 6.141359830345801 0.0028513310879101014 76-77 0.0 0.0 0.0 6.717048668657506 0.0029786226543346596 78-79 0.0 0.0 0.0 7.42323682086767 0.0030040809676195714 80-81 0.0 0.0 0.0 8.181869098444752 0.0030040809676195714 82-83 0.0 0.0 0.0 8.89508374512155 0.0031059142207592177 84-85 0.0 0.0 0.0 9.63872107617382 0.0031059142207592177 86-87 0.0 5.0916626569823246E-5 0.0 10.416268880521589 0.0031822891606139527 88-89 0.0 5.0916626569823246E-5 0.0 11.249799515782883 0.0032586641004686877 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 595 0.0 30.28238 3 TATCACG 90 9.042952E-4 26.34213 2 CATGGGG 3535 0.0 26.289295 4 GTACATG 15065 0.0 25.67372 1 CATGGGT 1385 0.0 24.9911 4 ATGGGTA 805 0.0 24.738064 5 TACATGG 15395 0.0 24.670399 2 ACATGGG 15005 0.0 24.616417 3 GTAAGCG 185 0.0 24.415956 94-95 TAGGGTC 625 0.0 24.276287 4 GTATAGG 725 0.0 24.219437 1 GACATAT 745 0.0 24.206257 1 CGTGCAA 510 0.0 24.156136 9 ATGGGGA 1915 0.0 23.76921 5 GAGTACT 9190 0.0 23.355265 12-13 CATTCCG 500 0.0 22.74393 9 AGTACTT 9620 0.0 22.729963 12-13 ACCGTGC 535 0.0 22.14337 7 TAAGGTG 775 0.0 22.024857 5 GTACTTT 10245 0.0 20.932156 14-15 >>END_MODULE