##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939327_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963995 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.295349529912244 33.0 31.0 34.0 31.0 34.0 2 32.48201904791 34.0 31.0 34.0 31.0 34.0 3 32.57189962296238 34.0 31.0 34.0 31.0 34.0 4 36.08221405858976 37.0 35.0 37.0 35.0 37.0 5 36.0344552811998 37.0 35.0 37.0 35.0 37.0 6 36.099671842341756 37.0 35.0 37.0 35.0 37.0 7 36.049080573015715 37.0 35.0 37.0 35.0 37.0 8 36.03607035659459 37.0 35.0 37.0 35.0 37.0 9 37.71071362197969 39.0 37.0 39.0 35.0 39.0 10-11 37.638361604790234 39.0 37.0 39.0 35.0 39.0 12-13 37.590582206166516 39.0 37.0 39.0 35.0 39.0 14-15 38.80589996410378 40.0 38.0 41.0 35.0 41.0 16-17 38.837287773135884 40.0 38.0 41.0 34.5 41.0 18-19 38.99806007652769 40.0 38.5 41.0 35.0 41.0 20-21 38.99741267162085 40.0 38.5 41.0 35.0 41.0 22-23 38.912191986232145 40.0 38.0 41.0 35.0 41.0 24-25 38.82713016071833 40.0 38.0 41.0 35.0 41.0 26-27 38.64422032642649 40.0 38.0 41.0 34.0 41.0 28-29 38.49655625396195 40.0 38.0 41.0 34.0 41.0 30-31 38.300359725966715 40.0 38.0 41.0 34.0 41.0 32-33 38.20094323050721 40.0 38.0 41.0 34.0 41.0 34-35 38.060343330812955 40.0 37.0 41.0 33.0 41.0 36-37 37.8698568479044 40.0 37.0 41.0 33.0 41.0 38-39 37.71894709507916 40.0 37.0 41.0 33.0 41.0 40-41 37.57229473598456 40.0 37.0 41.0 33.0 41.0 42-43 37.349063515945815 40.0 37.0 41.0 32.5 41.0 44-45 37.03967703583767 40.0 36.0 41.0 31.5 41.0 46-47 36.65211571312554 40.0 36.0 41.0 30.5 41.0 48-49 36.26046094822034 40.0 36.0 41.0 30.0 41.0 50-51 36.00960924034939 39.0 35.0 41.0 29.0 41.0 52-53 35.80116751824724 39.0 35.0 41.0 28.5 41.0 54-55 35.58473366785557 39.0 35.0 40.5 28.0 41.0 56-57 35.26927996252536 38.5 35.0 40.0 27.5 41.0 58-59 34.97875478298061 38.0 35.0 40.0 26.5 41.0 60-61 34.59676857629475 38.0 34.0 40.0 26.0 41.0 62-63 34.29536684156528 37.5 34.0 40.0 26.0 41.0 64-65 34.009884190132865 37.0 34.0 39.5 25.5 41.0 66-67 33.5738683143287 36.5 33.0 39.0 24.0 40.5 68-69 33.08581411867138 36.0 33.0 39.0 22.5 40.0 70-71 32.58903815946579 35.0 32.0 38.5 22.0 40.0 72-73 32.091881598476576 35.0 32.0 37.5 20.5 39.5 74-75 31.583191912403034 35.0 31.0 37.0 19.0 39.0 76-77 29.615215924684126 33.0 28.5 35.0 13.0 37.0 78-79 30.572460469604046 34.0 30.5 36.0 13.5 38.0 80-81 30.553869027161475 34.0 31.0 35.5 12.5 37.0 82-83 30.213177986705666 34.0 30.5 35.0 7.5 37.0 84-85 29.794465108108728 34.0 30.0 35.0 4.5 36.0 86-87 29.284926896453403 34.0 29.5 35.0 2.0 36.0 88-89 28.715018877339304 34.0 29.0 35.0 2.0 35.5 90-91 28.251469835717504 33.5 29.0 35.0 2.0 35.0 92-93 27.943916608749003 33.0 28.0 35.0 2.0 35.0 94-95 27.474056196680746 33.0 27.0 35.0 2.0 35.0 96-97 26.792103085802154 33.0 25.5 34.5 2.0 35.0 98-99 25.919730956545205 33.0 24.5 34.0 2.0 35.0 100-101 21.79665706888256 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19237952682953363 1101 2 0.21255746578118817 1101 3 0.2465502507112589 1101 4 0.13993572101291818 1101 5 0.16578734175026 1101 6 0.15424706751113604 1101 7 0.1669488396721448 1101 8 0.20212542516709675 1101 9 0.26520811668029154 1101 10-11 0.33497601500035046 1101 12-13 0.3153124308147497 1101 14-15 0.3678462271081244 1101 16-17 0.330719278181526 1101 18-19 0.39751735978730807 1101 20-21 0.2853373288791943 1101 22-23 0.2974988732482515 1101 24-25 0.31503440809343175 1101 26-27 0.30679674611447894 1101 28-29 0.4485627865744064 1101 30-31 0.48276446286821795 1101 32-33 0.5039652178765621 1101 34-35 0.5411698611038105 1101 36-37 0.5262788394457303 1101 38-39 0.584528675729004 1101 40-41 0.5832932620545961 1101 42-43 0.49960681669461593 1101 44-45 0.5644884436967743 1101 46-47 0.7053930773571722 1101 48-49 0.7053086493249623 1101 50-51 0.7918584178060968 1101 52-53 0.7715290282269294 1101 54-55 0.839486508663299 1101 56-57 0.8547723414639208 1101 58-59 0.7884733167580649 1101 60-61 0.8387857820222138 1101 62-63 0.863463747029833 1101 64-65 0.6843122573018903 1101 66-67 0.7920267550401903 1101 68-69 0.7732168609191987 1101 70-71 0.7022336283571846 1101 72-73 0.7958204326643354 1101 74-75 0.7167950912837568 1101 76-77 0.7879663852848715 1101 78-79 0.8360989132347747 1101 80-81 0.7945587264753051 1101 82-83 0.7631679216542295 1101 84-85 0.740664373157923 1101 86-87 0.6964803403438431 1101 88-89 0.8804767141967318 1101 90-91 1.0095737855811642 1101 92-93 0.917143392827299 1101 94-95 0.9169011245072589 1101 96-97 0.5193900911942428 1101 98-99 0.16091276633488505 1101 100-101 0.41663001631834007 1102 1 0.1521299295614611 1102 2 0.1816388079778406 1102 3 0.195078858084635 1102 4 0.08708935546746943 1102 5 0.1749380975563568 1102 6 0.2008170356143424 1102 7 0.13486864078370786 1102 8 0.19058616336238998 1102 9 0.22718078453531376 1102 10-11 0.2226990727170346 1102 12-13 0.22589843526357356 1102 14-15 0.2523324141001453 1102 16-17 0.2435414905365505 1102 18-19 0.2072965061028711 1102 20-21 0.3443297804709218 1102 22-23 0.2864106068509997 1102 24-25 0.3000298284254441 1102 26-27 0.2802314542062234 1102 28-29 0.2857239501318318 1102 30-31 0.48742725129613973 1102 32-33 0.42751747435513465 1102 34-35 0.35317234506425166 1102 36-37 0.41174048499443927 1102 38-39 0.3124486698944864 1102 40-41 0.38637719117377856 1102 42-43 0.5131050395260317 1102 44-45 0.5645672740474197 1102 46-47 0.4611691433901939 1102 48-49 0.4434878505536446 1102 50-51 0.56322519665855 1102 52-53 0.6083893494257495 1102 54-55 0.6200431903667223 1102 56-57 0.5464175040689483 1102 58-59 0.49099760401372095 1102 60-61 0.3997570362935292 1102 62-63 0.503145765773219 1102 64-65 0.5383538411162547 1102 66-67 0.442130467896078 1102 68-69 0.43676838808296026 1102 70-71 0.24568470313125346 1102 72-73 0.32599851418027725 1102 74-75 0.3499819181422765 1102 76-77 0.4457767033733795 1102 78-79 0.5387771073655045 1102 80-81 0.6355884760843722 1102 82-83 0.6292689403354146 1102 84-85 0.522186324461142 1102 86-87 0.598818652323267 1102 88-89 0.558752892844538 1102 90-91 0.5390536554199237 1102 92-93 0.4523188895657597 1102 94-95 0.37337926329586324 1102 96-97 0.13521064543060035 1102 98-99 0.24883971044148367 1102 100-101 -0.053390854955456035 1103 1 0.14633437002398608 1103 2 0.19521466510158803 1103 3 0.20592025636058509 1103 4 0.13388002159906165 1103 5 0.1375827143432602 1103 6 0.06567130668973675 1103 7 0.219568425822267 1103 8 0.12957568904732852 1103 9 0.17620288107819704 1103 10-11 0.13957339026890025 1103 12-13 0.19525165330788496 1103 14-15 0.2966017162998398 1103 16-17 0.18909340636872685 1103 18-19 0.1277016664217996 1103 20-21 0.19823532809375166 1103 22-23 0.2102330630110032 1103 24-25 0.22126714772250722 1103 26-27 0.21301478489531434 1103 28-29 0.20027911060630288 1103 30-31 0.2037901410498577 1103 32-33 0.23897980321813606 1103 34-35 0.2675392976203028 1103 36-37 0.26615310519085966 1103 38-39 0.38812691834760216 1103 40-41 0.376213963579076 1103 42-43 0.3344055095791916 1103 44-45 0.4253727417207642 1103 46-47 0.47784809801186867 1103 48-49 0.44047496842157585 1103 50-51 0.40346778266645345 1103 52-53 0.2506932009996987 1103 54-55 0.3490735618362635 1103 56-57 0.5279824606878023 1103 58-59 0.4656626405820603 1103 60-61 0.5176420770304375 1103 62-63 0.40020536702130727 1103 64-65 0.4889484848122194 1103 66-67 0.28565813757095526 1103 68-69 0.32464935357402425 1103 70-71 0.445475963439911 1103 72-73 0.7313314866521523 1103 74-75 0.5877678464117295 1103 76-77 0.5562951928570676 1103 78-79 0.5215506387993365 1103 80-81 0.6249508401002615 1103 82-83 0.47598370112470434 1103 84-85 0.49315564190412786 1103 86-87 0.48082939728442753 1103 88-89 0.5214006205061281 1103 90-91 0.6404370613921806 1103 92-93 0.6188642321197264 1103 94-95 0.47198310273740063 1103 96-97 0.3111788274043228 1103 98-99 0.011086446356220137 1103 100-101 -0.20161625649160086 1104 1 0.19051273459388085 1104 2 0.15800192766576515 1104 3 0.20198267890150134 1104 4 0.08728569223669069 1104 5 0.12010743801981505 1104 6 0.1114249045853768 1104 7 0.12580511550534368 1104 8 0.13066934815145714 1104 9 0.21941101523101736 1104 10-11 0.14228831552878063 1104 12-13 0.15823923456088806 1104 14-15 0.1766988405126071 1104 16-17 0.23671310437313053 1104 18-19 0.2848071625393658 1104 20-21 0.21133900313231635 1104 22-23 0.2476526952542244 1104 24-25 0.19546448186520848 1104 26-27 0.1284615815667891 1104 28-29 0.044404360372347185 1104 30-31 0.08076006795376856 1104 32-33 0.1135393297375984 1104 34-35 0.26627162430163764 1104 36-37 0.19885614285178832 1104 38-39 0.27743769053568457 1104 40-41 0.3411313827054627 1104 42-43 0.29679668746319976 1104 44-45 0.35979164047382994 1104 46-47 0.31925589324164605 1104 48-49 0.28447780121111066 1104 50-51 0.25222413747334826 1104 52-53 0.39895381763727755 1104 54-55 0.3789245874578313 1104 56-57 0.3844050521843698 1104 58-59 0.2798038247892549 1104 60-61 0.3920286010459151 1104 62-63 0.3812924500068604 1104 64-65 0.36347513073882 1104 66-67 0.36557669487171296 1104 68-69 0.3287366641944587 1104 70-71 0.33318115098575873 1104 72-73 0.27023732169274695 1104 74-75 0.25648221523081105 1104 76-77 0.31595728153349967 1104 78-79 0.44556105941800084 1104 80-81 0.6196944979397543 1104 82-83 0.47820794676803047 1104 84-85 0.5336523917839884 1104 86-87 0.5300491155230382 1104 88-89 0.7669210191200833 1104 90-91 0.7419470393874796 1104 92-93 0.646187498933088 1104 94-95 0.6289348188514197 1104 96-97 0.5888266003764748 1104 98-99 0.6150827030271877 1104 100-101 0.8200297965726158 1105 1 0.08608693765091147 1105 2 0.10672214382887546 1105 3 0.06548328507736301 1105 4 0.032615760936621996 1105 5 -0.01768734966015728 1105 6 0.11121032625398897 1105 7 0.10096188922346272 1105 8 0.06756747098724958 1105 9 0.17772861968740017 1105 10-11 0.1281971435095457 1105 12-13 0.19533307692346824 1105 14-15 0.20843234500147645 1105 16-17 0.24484679586527136 1105 18-19 0.22463695786586868 1105 20-21 0.2575047228968259 1105 22-23 0.1471179926765842 1105 24-25 0.19046198584695873 1105 26-27 0.20909913594432794 1105 28-29 0.2591242120375412 1105 30-31 0.38795668287757934 1105 32-33 0.28114037885765697 1105 34-35 0.3415741971814086 1105 36-37 0.28860630331009673 1105 38-39 0.36422670171625526 1105 40-41 0.4550439507516799 1105 42-43 0.4676956502289471 1105 44-45 0.4503395759101636 1105 46-47 0.5160410217271618 1105 48-49 0.6489256265056795 1105 50-51 0.663675393802535 1105 52-53 0.6999780567479021 1105 54-55 0.6762315818556601 1105 56-57 0.7443289830565547 1105 58-59 0.7743276399729737 1105 60-61 0.7121785206186573 1105 62-63 0.7067730721889447 1105 64-65 0.584577340705458 1105 66-67 0.5891524193112971 1105 68-69 0.5777537084334128 1105 70-71 0.4862140112642024 1105 72-73 0.5778215722137432 1105 74-75 0.5984796637454792 1105 76-77 0.6721947122987046 1105 78-79 0.5910765466169927 1105 80-81 0.6245507193643078 1105 82-83 0.7436208991218116 1105 84-85 0.8483823859995638 1105 86-87 0.8444562913774298 1105 88-89 0.7268403541491253 1105 90-91 0.7967627698492024 1105 92-93 0.6823406197482953 1105 94-95 0.7876007565222771 1105 96-97 0.86181350222353 1105 98-99 0.8574769788253214 1105 100-101 0.8198288472932909 1106 1 0.056695206163965395 1106 2 0.09198495246658211 1106 3 0.11954373524119433 1106 4 0.12653670294667307 1106 5 0.059335279237870964 1106 6 0.06866158803810407 1106 7 0.030425301334126686 1106 8 0.03698799959245491 1106 9 0.09446314658784871 1106 10-11 0.1404787309246771 1106 12-13 0.15695450987592352 1106 14-15 0.14399202915482334 1106 16-17 0.1561296114540056 1106 18-19 0.08373190897528104 1106 20-21 0.15178442479343346 1106 22-23 0.20831751781846464 1106 24-25 0.2657522091271929 1106 26-27 0.2651705680197267 1106 28-29 0.2795728120355818 1106 30-31 0.23829937334186013 1106 32-33 0.2511079516256771 1106 34-35 0.23233824841209127 1106 36-37 0.2959273299336118 1106 38-39 0.4053763150244123 1106 40-41 0.30614923267118144 1106 42-43 0.24767121757172106 1106 44-45 0.5305668727598132 1106 46-47 0.5676082586576428 1106 48-49 0.5669555183068908 1106 50-51 0.5873145694188722 1106 52-53 0.7186058024116093 1106 54-55 0.626866049027214 1106 56-57 0.6612502185082221 1106 58-59 0.60044831494222 1106 60-61 0.5882429897732422 1106 62-63 0.5872659233028443 1106 64-65 0.568975284414023 1106 66-67 0.531819217982104 1106 68-69 0.6354227175788552 1106 70-71 0.6348881278399077 1106 72-73 0.6213539493495475 1106 74-75 0.5315665001682817 1106 76-77 0.5189608042419636 1106 78-79 0.631316550351162 1106 80-81 0.679685041004273 1106 82-83 0.7814412730086602 1106 84-85 0.7091088071325942 1106 86-87 0.6206229150832918 1106 88-89 0.8503586151670817 1106 90-91 0.9471767247079477 1106 92-93 1.0776204610817075 1106 94-95 1.1600386796453286 1106 96-97 0.9981544486872345 1106 98-99 1.2366823436636416 1106 100-101 1.2096337873993868 1107 1 0.05744257700497002 1107 2 0.01657345313548575 1107 3 0.12170865054905988 1107 4 0.04664267521166465 1107 5 0.08821976251279295 1107 6 0.12258681193123522 1107 7 0.1055704278569678 1107 8 0.07452790249362806 1107 9 0.0816407739865852 1107 10-11 0.12149245760750915 1107 12-13 0.1384246332811898 1107 14-15 0.1570782296012183 1107 16-17 0.14093702985188372 1107 18-19 0.12236487354345371 1107 20-21 0.21671194848492092 1107 22-23 0.20667686491665194 1107 24-25 0.1987365431824557 1107 26-27 0.20831100163054828 1107 28-29 0.2717538369601016 1107 30-31 0.20393897771885605 1107 32-33 0.11439125946696294 1107 34-35 0.11837183004899288 1107 36-37 0.09196338018475814 1107 38-39 0.11099576329336713 1107 40-41 0.163476902613219 1107 42-43 0.049560258875622765 1107 44-45 -0.12108154227955481 1107 46-47 -0.18020328512548645 1107 48-49 -0.20031226865255292 1107 50-51 -0.09364079277604276 1107 52-53 -0.043126265566051813 1107 54-55 -0.0941075860030125 1107 56-57 -0.10946684182182054 1107 58-59 -0.03803967155929655 1107 60-61 -0.0859618577028769 1107 62-63 -0.07859992829696694 1107 64-65 -0.053699573640656695 1107 66-67 -0.15364327392703814 1107 68-69 -0.02208971668686388 1107 70-71 -0.007509346199348954 1107 72-73 0.026093434198109833 1107 74-75 0.07325808626565333 1107 76-77 0.1754743404767254 1107 78-79 0.21380813712719515 1107 80-81 0.15860426302600672 1107 82-83 0.17356664807260103 1107 84-85 0.14325152357534776 1107 86-87 0.34886708563314883 1107 88-89 0.5116613478636651 1107 90-91 0.6324080491322128 1107 92-93 0.5598468626989472 1107 94-95 0.5584199982585965 1107 96-97 0.6923758553901571 1107 98-99 0.6966717057177298 1107 100-101 0.7490030946349719 1108 1 0.04850398537506351 1108 2 0.020679962365051097 1108 3 0.05093723653028093 1108 4 0.07048808488021052 1108 5 0.029422975323036837 1108 6 0.03177457574569331 1108 7 0.0473531915699823 1108 8 0.06309919072503334 1108 9 0.055762531464687015 1108 10-11 0.05598024779673949 1108 12-13 0.11415730735200924 1108 14-15 0.06725251131580734 1108 16-17 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-0.3947920075116933 2316 86-87 -0.5803396153451708 2316 88-89 -0.9773492639626724 2316 90-91 -1.1176171621566091 2316 92-93 -0.7879629141781095 2316 94-95 -0.5823580160395458 2316 96-97 -0.41064719076533507 2316 98-99 -0.25412527475961966 2316 100-101 0.26461465367677306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 13.0 5 51.0 6 107.0 7 347.0 8 627.0 9 1176.0 10 2032.0 11 3314.0 12 5046.0 13 7032.0 14 8596.0 15 11732.0 16 15532.0 17 22860.0 18 20977.0 19 11985.0 20 10527.0 21 10951.0 22 11535.0 23 12723.0 24 14706.0 25 17066.0 26 20334.0 27 24418.0 28 30035.0 29 36283.0 30 44276.0 31 54252.0 32 67486.0 33 87159.0 34 120128.0 35 176330.0 36 278526.0 37 461340.0 38 352365.0 39 22120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43756686628887 21.007415487394283 14.899507149171253 20.655510497145592 2 14.802634426258724 23.15041535238124 38.94062866758826 23.106321553771778 3 20.545469820442516 27.02333763578828 30.928541060440583 21.502651483328624 4 11.838268427363612 17.53461694148916 39.78105850574976 30.84605612539747 5 11.742341502906067 40.421487834744994 34.31276556203045 13.523405100318483 6 28.185611470497633 38.74857115216688 17.740523779337526 15.325293597997957 7 26.62175820203208 35.16658647297982 21.51502422358509 16.69663110140301 8 25.53366989223496 32.96036904370938 20.471131545650575 21.034829518405086 9 26.085351541118996 18.506666259333652 22.766707654551055 32.64127454499629 10-11 24.366889936074177 27.0916422903317 27.517610788214835 21.02385698537929 12-13 26.74019027543349 25.586623183867573 27.457274585729596 20.215911954969336 14-15 23.00363321786094 26.636418280093576 28.39085694907971 21.96909155296578 16-17 20.042561576555336 30.06800483508471 28.720713775816225 21.168719812543724 18-19 19.731496193090152 29.525922806810307 31.084557517854556 19.658023482244985 20-21 21.511623762598052 27.25390199766394 30.221859129915874 21.01261510982213 22-23 20.720597557529423 27.452819380904735 30.493789444474146 21.332793617091696 24-25 21.657438028100888 27.89503028266365 30.42607033113628 20.02146135809918 26-27 21.18642449682242 26.682623949621014 32.356878981524126 19.77407257203244 28-29 19.627544876641743 28.842766911321053 31.5894134150036 19.940274797033595 30-31 20.233325441256213 27.54943367982098 31.07810865098944 21.13913222793337 32-33 20.384445988915452 27.96063635599887 31.031494479364763 20.623423175720916 34-35 21.726543867402864 27.223004087651304 30.753440490762564 20.297011554183275 36-37 21.38333955411032 26.486175871973867 31.638557750621356 20.49192682329446 38-39 20.487215841465183 28.08186831078124 31.276399492956614 20.154516354796964 40-41 20.2903668385245 28.121325129033398 31.244986136213136 20.343321896228964 42-43 19.96536135721565 28.434338946986276 29.76520010397194 21.835099591826136 44-45 20.677734384944777 27.384710560751945 29.940528623145973 21.9970264311573 46-47 21.835190307498628 28.49348966454811 29.260549620225127 20.410770407728133 48-49 21.298582602995182 28.189945200771387 29.320782336954792 21.190689859278635 50-51 19.670553983362474 28.56602413245662 30.60874931389811 21.154672570282802 52-53 20.741738518549447 29.650243330682276 28.57758221257295 21.03043593819533 54-55 19.921970269781745 28.333906145382244 29.241316805796348 22.50280677903966 56-57 21.253980441736108 28.276538739232315 28.55851745793486 21.910963361096712 58-59 22.230295500660656 27.529143997698526 28.684873125911743 21.555687375729075 60-61 20.97406796403222 28.429999287161785 30.008197639487165 20.58773510931883 62-63 20.549518756113248 28.293897115693547 29.847406252529947 21.30917787566326 64-65 20.107984064102045 29.386858968728767 29.054984143277462 21.450172823891727 66-67 20.686140154222038 28.24897217979859 28.224277509035833 22.840610156943544 68-69 20.05665064637033 28.31244109498785 28.980069420718372 22.650838837923448 70-71 21.208658881514463 28.431309652010317 28.532582822257694 21.827448644217526 72-73 21.15069030216472 27.144391915458034 29.664637639097858 22.040280143279386 74-75 19.939434672695196 29.673115257421735 28.39785742835394 21.98959264152913 76-77 20.363374808695024 27.999519121309053 28.76034884635058 22.876757223645345 78-79 20.37530222269405 27.99519651444343 29.06864875364723 22.560852509215295 80-81 20.914311027155144 28.037377943143877 28.63407064945513 22.41424038024585 82-83 20.46270484395327 27.693960524339424 29.312981957693374 22.530352674013937 84-85 19.92729105725829 27.927031382462786 28.90399415477127 23.24168340550765 86-87 19.85771174075655 28.16688223563068 28.899884675913835 23.075521347698935 88-89 19.872627839163133 27.842229631225347 28.21153836816749 24.073604161444035 90-91 20.061862423504863 26.931215837107192 28.904956984528525 24.101964754859427 92-93 20.643713451408992 27.802031064233866 28.81277701827143 22.741478466085706 94-95 20.065351490202367 26.9817896685073 29.101296082729334 23.85156275856099 96-97 19.188516264043443 26.90589843660498 29.75239244499095 24.15319285436063 98-99 19.698675020398497 27.4823096320378 28.073299668914213 24.745715678649486 100-101 19.26648737161327 26.34221797689192 29.427521960419313 24.963772691075505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1496.0 1 1036.0 2 745.5 3 931.5 4 783.5 5 802.5 6 1529.0 7 3088.0 8 4579.5 9 4770.0 10 3854.0 11 3194.0 12 3226.0 13 3338.5 14 3374.0 15 3437.5 16 3523.0 17 3638.5 18 3853.5 19 4122.0 20 4435.5 21 5011.0 22 5741.5 23 6547.5 24 7369.0 25 8996.5 26 12422.5 27 15943.5 28 17639.0 29 19831.0 30 24417.5 31 29007.5 32 35979.0 33 44202.0 34 51895.0 35 57675.5 36 64769.5 37 72431.5 38 71650.5 39 68171.5 40 64879.0 41 63596.5 42 63242.0 43 64150.0 44 66689.0 45 67374.0 46 66901.5 47 81913.0 48 123509.5 49 117726.0 50 74200.0 51 55503.5 52 45793.5 53 41566.5 54 39372.0 55 36845.0 56 32982.0 57 28766.5 58 26025.5 59 23640.0 60 21057.5 61 18479.0 62 15517.0 63 11638.5 64 8283.5 65 6242.5 66 4543.5 67 3561.5 68 2755.5 69 1892.0 70 1452.0 71 1197.0 72 989.0 73 848.5 74 692.5 75 515.5 76 330.5 77 196.0 78 141.5 79 108.0 80 67.5 81 38.5 82 25.5 83 15.5 84 8.5 85 5.5 86 2.0 87 0.0 88 1.0 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29511276759869554 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0017057069900890786 16-17 8.655826516869951E-4 18-19 2.545831328491162E-5 20-21 5.091662656982324E-5 22-23 0.0 24-25 0.0 26-27 7.637493985473486E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015784154236645205 36-37 0.0074338274791941935 38-39 0.0030804559074743064 40-41 0.0033350390403234227 42-43 1.7820819299438134E-4 44-45 0.0012729156642455812 46-47 1.272915664245581E-4 48-49 3.818746992736743E-4 50-51 5.091662656982324E-5 52-53 5.091662656982324E-5 54-55 0.0 56-57 8.655826516869951E-4 58-59 0.0020366650627929297 60-61 7.637493985473486E-4 62-63 0.0011201657845361112 64-65 7.637493985473486E-5 66-67 4.3279132584349755E-4 68-69 0.0014256655439550507 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2587073796012719 78-79 2.291248195642046E-4 80-81 1.272915664245581E-4 82-83 0.0 84-85 0.0 86-87 0.10857970616014807 88-89 0.37507732962660295 90-91 0.2777756562516707 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.272915664245581E-4 100-101 3.564163859887627E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1963995.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.7045188351338 #Duplication Level Percentage of deduplicated Percentage of total 1 66.94022870725043 18.545468270481834 2 14.221274409192638 7.879871294581675 3 6.137000148211593 5.100679085920409 4 3.33034873427878 3.690628369455619 5 2.081017127798624 2.882678910666651 6 1.3385397095607117 2.2250159157059555 7 0.952234113895564 1.8466831560713621 8 0.7012028760456742 1.5541190629325896 9 0.4976426209799579 1.2408254429494134 >10 3.083200422573246 16.33758508227109 >50 0.383355433161434 7.458702672168206 >100 0.3021241650389572 16.352826718864744 >500 0.021159688910126317 3.9282790064888773 >1k 0.0080958809743092 4.1794356162961845 >5k 0.0018399729487066363 3.500288160955663 >10k+ 7.359891794826546E-4 3.2769132341897564 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27513 1.400869146815547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13738 0.6994926158162317 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12130 0.6176186802919559 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10903 0.5551439794907828 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9280 0.4725062945679597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8997 0.4580968892486997 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7487 0.3812127831282666 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6816 0.34704772669991524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6781 0.3452656447699714 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6602 0.33615156861397305 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6429 0.3273429922173936 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5861 0.298422348325734 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5365 0.2731677015471017 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5048 0.2570271309244677 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4469 0.2275464041405401 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4066 0.2070270036329013 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3793 0.19312676457933955 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3400 0.17311653033739902 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3242 0.16507170333936694 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3144 0.16008187393552428 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3065 0.15605946043650826 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2706 0.1377803914979417 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2414 0.1229127365395533 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2411 0.12275998665984385 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2389 0.12163982087530774 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2355 0.11990865557193374 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2333 0.11878848978739763 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2297 0.116955491230884 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2008 0.10224058615220506 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1979 0.1007640039816802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.0916626569823246E-5 2 0.0 0.0 0.0 0.0 5.0916626569823246E-5 3 0.0 0.0 0.0 0.0 5.0916626569823246E-5 4 0.0 0.0 0.0 0.0 5.0916626569823246E-5 5 0.0 0.0 0.0 0.0 1.0183325313964649E-4 6 0.0 0.0 0.0 0.0 1.5274987970946972E-4 7 0.0 0.0 0.0 0.0 1.5274987970946972E-4 8 0.0 0.0 0.0 0.0 1.5274987970946972E-4 9 0.0 0.0 0.0 0.0 1.5274987970946972E-4 10-11 0.0 0.0 0.0 0.0 1.7820819299438134E-4 12-13 0.0 0.0 0.0 0.0 4.8370795241332084E-4 14-15 0.0 0.0 0.0 2.5458313284911623E-5 6.364578321227906E-4 16-17 0.0 0.0 0.0 1.272915664245581E-4 7.128327719775254E-4 18-19 0.0 0.0 0.0 2.0366650627929298E-4 8.655826516869951E-4 20-21 0.0 0.0 0.0 4.0733301255858596E-4 0.0011965407243908462 22-23 0.0 0.0 0.0 0.0010947074712511996 0.0012729156642455812 24-25 0.0 0.0 0.0 0.002622206268345897 0.0012729156642455812 26-27 0.0 0.0 0.0 0.005218954223406883 0.0015529571103796089 28-29 0.0 0.0 0.0 0.012118157123617933 0.0015784154236645205 30-31 0.0 0.0 0.0 0.030320851122329743 0.001603873736949432 32-33 0.0 0.0 0.0 0.06741361357844597 0.001680248676804167 34-35 0.0 0.0 0.0 0.11886486472725236 0.001680248676804167 36-37 0.0 0.0 0.0 0.20124796651722637 0.001680248676804167 38-39 0.0 0.0 0.0 0.324644411009193 0.0017311653033739902 40-41 0.0 0.0 0.0 0.45575472442648784 0.0017311653033739902 42-43 0.0 0.0 0.0 0.5923131168867538 0.001756623616658902 44-45 0.0 0.0 0.0 0.758454069384087 0.0018584568697985484 46-47 0.0 0.0 0.0 0.9631389081947765 0.0020366650627929297 48-49 0.0 0.0 0.0 1.1704204949605268 0.002240331569072223 50-51 0.0 0.0 0.0 1.4000290224771448 0.0024949147019213387 52-53 0.0 0.0 0.0 1.6278554680638189 0.0025967479550609855 54-55 0.0 0.0 0.0 1.8731463165639424 0.002698581208200632 56-57 0.0 0.0 0.0 2.189007609489841 0.002698581208200632 58-59 0.0 0.0 0.0 2.5160451019478156 0.002698581208200632 60-61 0.0 0.0 0.0 2.8855979775915928 0.002749497834770455 62-63 0.0 0.0 0.0 3.249957357325248 0.0028004144613402782 64-65 0.0 0.0 0.0 3.6178809009187907 0.0028513310879101014 66-67 0.0 0.0 0.0 4.044511314947339 0.0028767894011950132 68-69 0.0 0.0 0.0 4.5396245917123 0.002902247714479925 70-71 0.0 0.0 0.0 5.085145328781387 0.002902247714479925 72-73 0.0 0.0 0.0 5.609510207510711 0.003029539280904483 74-75 0.0 0.0 0.0 6.172699013999527 0.0031822891606139523 76-77 0.0 0.0 0.0 6.750577267253735 0.0032586641004686877 78-79 0.0 0.0 0.0 7.458089251754714 0.003309580727038511 80-81 0.0 0.0 0.0 8.218860027647729 0.003360497353608334 82-83 0.0 0.0 0.0 8.932583840590226 0.0035896221731725386 84-85 0.0 0.0 0.0 9.67833421164514 0.0036150804864574504 86-87 0.0 0.0 0.0 10.457587722983 0.0036659971130272736 88-89 0.0 0.0 0.0 11.293460523066503 0.0037169137395970967 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 655 0.0 31.878237 9 CATATAG 535 0.0 30.156845 3 TATAACG 85 6.446264E-4 27.913343 2 TAGGCAT 845 0.0 27.516941 5 GTAAGCG 210 0.0 27.115822 94-95 GTACATG 14680 0.0 25.64524 1 TACATGG 14980 0.0 24.740017 2 ACATGGG 14645 0.0 24.00986 3 CCTTTAG 900 0.0 23.268398 1 CATGGGG 3820 0.0 23.229456 4 CTTTAGG 925 0.0 23.08509 2 TAGGGTC 560 0.0 22.878973 4 AGTACTT 9930 0.0 22.38944 12-13 GACATAT 875 0.0 22.301401 1 TTAGGCA 1050 0.0 22.144587 4 CATGGGA 9815 0.0 21.804544 4 ATGGGAG 3245 0.0 21.788754 5 GAGTACT 9505 0.0 21.71801 12-13 CGTCTAG 110 0.002845097 21.63384 1 GTATAGG 800 0.0 20.227642 1 >>END_MODULE