##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939326_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2002064 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08031361634793 33.0 31.0 34.0 30.0 34.0 2 32.132629626225736 34.0 31.0 34.0 30.0 34.0 3 32.1882172597879 34.0 31.0 34.0 30.0 34.0 4 35.66923534911971 37.0 35.0 37.0 35.0 37.0 5 35.73220586354882 37.0 35.0 37.0 35.0 37.0 6 35.85325593987005 37.0 36.0 37.0 35.0 37.0 7 35.79950590990098 37.0 35.0 37.0 35.0 37.0 8 35.793929664586145 37.0 35.0 37.0 35.0 37.0 9 37.431148055207025 39.0 37.0 39.0 35.0 39.0 10-11 37.35569816948909 39.0 37.0 39.0 34.0 39.0 12-13 37.295790494209974 39.0 37.0 39.0 34.0 39.0 14-15 38.507533974937864 40.0 38.0 41.0 34.0 41.0 16-17 38.48571399315906 40.0 38.0 41.0 34.0 41.0 18-19 38.508118621582526 40.0 38.0 41.0 34.0 41.0 20-21 38.440183480647974 40.0 38.0 41.0 34.0 41.0 22-23 38.363031101902834 40.0 38.0 41.0 34.0 41.0 24-25 38.36474882920825 40.0 38.0 41.0 34.0 41.0 26-27 38.269926186175866 40.0 38.0 41.0 34.0 41.0 28-29 38.16399750457528 40.0 38.0 41.0 34.0 41.0 30-31 37.994051139224325 40.0 38.0 41.0 33.0 41.0 32-33 37.774423295159394 40.0 37.0 41.0 33.0 41.0 34-35 37.4562264243301 40.0 37.0 41.0 32.5 41.0 36-37 37.18444290492212 40.0 36.5 41.0 31.5 41.0 38-39 36.87852036698128 39.5 35.5 41.0 30.5 41.0 40-41 36.794077512007604 39.5 36.0 41.0 31.0 41.0 42-43 36.59329272191099 39.5 36.0 41.0 30.5 41.0 44-45 36.46025102094639 40.0 36.0 41.0 30.0 41.0 46-47 36.17873204852592 40.0 36.0 41.0 29.5 41.0 48-49 35.907566141741725 40.0 36.0 41.0 28.0 41.0 50-51 34.722533095845094 38.0 34.0 40.0 25.5 40.5 52-53 34.874237786604226 38.5 34.5 39.5 26.5 40.5 54-55 35.41077258269466 39.0 35.0 41.0 27.0 41.0 56-57 35.30945489255089 39.0 35.0 41.0 26.5 41.0 58-59 35.13942461379856 39.0 35.0 41.0 26.0 41.0 60-61 34.814618313900056 38.5 35.0 40.5 26.0 41.0 62-63 34.50586469763205 38.0 34.5 40.0 25.0 41.0 64-65 34.139378411479356 37.5 34.0 40.0 23.0 41.0 66-67 33.741492279967076 37.0 34.0 39.5 22.5 41.0 68-69 33.31560879172694 36.5 34.0 39.0 22.0 41.0 70-71 32.868153315778116 36.0 33.0 39.0 20.5 40.5 72-73 32.421647359924556 35.0 33.0 38.0 19.5 40.0 74-75 31.98760129546308 35.0 32.0 37.0 18.0 39.5 76-77 31.562333921393122 35.0 32.0 37.0 14.5 39.0 78-79 31.141319158628296 35.0 31.5 36.0 9.5 39.0 80-81 30.664450537045767 35.0 31.0 36.0 6.5 37.5 82-83 30.229285627232695 34.5 31.0 35.5 2.0 37.0 84-85 29.823024638572992 34.0 30.5 35.0 2.0 36.5 86-87 29.578188309664426 34.0 30.5 35.0 2.0 36.0 88-89 29.16549396023304 34.0 30.0 35.0 2.0 36.0 90-91 28.761811060985064 34.0 29.0 35.0 2.0 35.5 92-93 28.38666096588321 34.0 29.0 35.0 2.0 35.0 94-95 27.9443391919539 34.0 29.0 35.0 2.0 35.0 96-97 27.407038436333703 34.0 27.5 35.0 2.0 35.0 98-99 26.775304635616045 34.0 25.5 35.0 2.0 35.0 100-101 24.420180623596448 31.5 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2569811690467745 1101 2 0.10913315747366426 1101 3 0.3002984540036735 1101 4 0.38146181324280093 1101 5 0.202142683118673 1101 6 0.2832016923746892 1101 7 0.31284297018756035 1101 8 0.24543540141151965 1101 9 0.3343660868413849 1101 10-11 0.35058468232266193 1101 12-13 0.3030814784509701 1101 14-15 0.47071969012825576 1101 16-17 0.47909442347333453 1101 18-19 0.4435057730672085 1101 20-21 0.4268774980587082 1101 22-23 0.4253475610829227 1101 24-25 0.5573323989098071 1101 26-27 0.5028696804290718 1101 28-29 0.4370098523134587 1101 30-31 0.5114571173652109 1101 32-33 0.4320381648006517 1101 34-35 0.3760879282928826 1101 36-37 0.42680378305390576 1101 38-39 0.49764210928616137 1101 40-41 0.2796559484489336 1101 42-43 0.3106658748327078 1101 44-45 0.41148463328497087 1101 46-47 0.4860907367066716 1101 48-49 0.5687881698866377 1101 50-51 0.3656577923769859 1101 52-53 0.44113035787773924 1101 54-55 0.4655376087664038 1101 56-57 0.46209907897425495 1101 58-59 0.3334199094874464 1101 60-61 0.4457066318147511 1101 62-63 0.49846138615709634 1101 64-65 0.5516097279146877 1101 66-67 0.4842744640317207 1101 68-69 0.43098218063293814 1101 70-71 0.42074150492351947 1101 72-73 0.41448646847570103 1101 74-75 0.39656912254275056 1101 76-77 0.4263071137193535 1101 78-79 0.1323563940893031 1101 80-81 0.26726945343215647 1101 82-83 0.20243299752076283 1101 84-85 0.22928544152291508 1101 86-87 0.2344620151652137 1101 88-89 0.21965423270475526 1101 90-91 0.2705917348298996 1101 92-93 0.2768328609023669 1101 94-95 0.29934814933018217 1101 96-97 0.2108388326373607 1101 98-99 0.2096934116724114 1101 100-101 -0.14974892928071526 1102 1 0.37941977321844433 1102 2 0.35842775859495646 1102 3 0.4180005059447609 1102 4 0.39686478735698927 1102 5 0.3770992576621808 1102 6 0.44173426499759216 1102 7 0.3543442906038834 1102 8 0.4159816442269033 1102 9 0.3959137277446345 1102 10-11 0.4001956184766513 1102 12-13 0.47779550593257625 1102 14-15 0.6087642735969254 1102 16-17 0.588603892341176 1102 18-19 0.6246375887455642 1102 20-21 0.566129874101982 1102 22-23 0.5682900417709504 1102 24-25 0.62016126730596 1102 26-27 0.5563006445448124 1102 28-29 0.6413170927139689 1102 30-31 0.6824845707269844 1102 32-33 0.5958008570377302 1102 34-35 0.5843698336465692 1102 36-37 0.5686059041932694 1102 38-39 0.6353978958164959 1102 40-41 0.5071117815821964 1102 42-43 0.6058396472691996 1102 44-45 0.8624625886873787 1102 46-47 0.6960499944491119 1102 48-49 0.7503856618017366 1102 50-51 0.8720335673272004 1102 52-53 0.8151792344647077 1102 54-55 0.7751770009512384 1102 56-57 0.8584640505182293 1102 58-59 0.6588809960417947 1102 60-61 0.6927819449723103 1102 62-63 0.7159113586190031 1102 64-65 0.7708214323108962 1102 66-67 0.7414516681384313 1102 68-69 0.6763441300475819 1102 70-71 0.6785238410012511 1102 72-73 0.5115578980558482 1102 74-75 0.4474118588407734 1102 76-77 0.4241570240440211 1102 78-79 0.3471312878205062 1102 80-81 0.39079616538369066 1102 82-83 0.20473207534765692 1102 84-85 0.3282538221417468 1102 86-87 0.18455945765296633 1102 88-89 0.09982892307772318 1102 90-91 0.18085708908276743 1102 92-93 0.289413533396754 1102 94-95 0.22602232424650737 1102 96-97 0.23254732425589353 1102 98-99 0.18112793455727427 1102 100-101 0.3426141922681971 1103 1 0.23526652323472064 1103 2 0.2727406382679547 1103 3 0.34326064511964205 1103 4 0.2670843766848634 1103 5 0.2533672327052656 1103 6 0.23075411335109663 1103 7 0.20410770700129888 1103 8 0.21716096466148826 1103 9 0.34014783758384226 1103 10-11 0.3502937809707021 1103 12-13 0.31361179645244164 1103 14-15 0.45783866809023266 1103 16-17 0.4320525309147456 1103 18-19 0.4197135319106806 1103 20-21 0.5498472346444245 1103 22-23 0.5764027521864534 1103 24-25 0.44180258532160366 1103 26-27 0.44797327862313097 1103 28-29 0.39429503099322716 1103 30-31 0.4074103064317427 1103 32-33 0.3612178506271917 1103 34-35 0.3886951506617393 1103 36-37 0.44055284505251535 1103 38-39 0.5597511026292921 1103 40-41 0.5400339060035861 1103 42-43 0.3906157237160244 1103 44-45 0.533198173533691 1103 46-47 0.42313633290242336 1103 48-49 0.34912102736709016 1103 50-51 0.421370756350818 1103 52-53 0.49950047422446175 1103 54-55 0.5073450044323167 1103 56-57 0.4991036656801313 1103 58-59 0.44610462493617575 1103 60-61 0.5077432453084256 1103 62-63 0.43282374559507986 1103 64-65 0.35702608997562635 1103 66-67 0.3312470515888819 1103 68-69 0.5195853082716084 1103 70-71 0.39825244654563363 1103 72-73 0.27057822608646376 1103 74-75 0.24091668816138423 1103 76-77 0.24768124602487873 1103 78-79 0.08911245706772064 1103 80-81 0.18317512400468416 1103 82-83 0.024509162542670992 1103 84-85 0.031423117324759886 1103 86-87 0.006151094825199266 1103 88-89 -0.011702514617361714 1103 90-91 0.13980221059525633 1103 92-93 0.2437378225324096 1103 94-95 0.020563598748584155 1103 96-97 -0.09726957613915133 1103 98-99 -0.14294363326735748 1103 100-101 -0.2053665384170884 1104 1 0.0914682472407975 1104 2 0.24563870923115871 1104 3 0.21514375940150643 1104 4 0.20132887672674116 1104 5 0.22645831660033622 1104 6 0.18208936224673522 1104 7 0.19922775740317178 1104 8 0.2265384797474539 1104 9 0.23072043047586277 1104 10-11 0.20309228639509058 1104 12-13 0.20042974155665405 1104 14-15 0.2645635299767335 1104 16-17 0.3023478407005058 1104 18-19 0.3766607228441714 1104 20-21 0.4202845728140119 1104 22-23 0.44588230920356153 1104 24-25 0.37611445780196817 1104 26-27 0.27765029151129994 1104 28-29 0.2363394385345785 1104 30-31 0.2962384994301104 1104 32-33 0.2945883298455243 1104 34-35 0.30142235055395616 1104 36-37 0.32779323355476464 1104 38-39 0.4137750580833597 1104 40-41 0.3603643587099583 1104 42-43 0.4346791193597568 1104 44-45 0.49067888134489124 1104 46-47 0.3434857227266406 1104 48-49 0.23744301663848688 1104 50-51 0.36251637914528345 1104 52-53 0.42893603886012954 1104 54-55 0.36103756032412804 1104 56-57 0.29508717582059774 1104 58-59 0.3140673916490897 1104 60-61 0.2747183684907313 1104 62-63 0.3205557708105857 1104 64-65 0.2992879101968242 1104 66-67 0.31985200510190026 1104 68-69 0.2817460334175479 1104 70-71 0.15506343590078586 1104 72-73 0.2081931689291494 1104 74-75 0.25673282331413816 1104 76-77 0.19234662351082932 1104 78-79 0.16350801704193785 1104 80-81 0.09858083354571434 1104 82-83 0.13388922685604143 1104 84-85 0.16503358809422508 1104 86-87 0.007414783265907943 1104 88-89 0.20410051022303577 1104 90-91 0.17345201230178375 1104 92-93 0.19954696551857154 1104 94-95 0.24569679588033821 1104 96-97 0.45529185113685244 1104 98-99 0.38897451468251987 1104 100-101 0.1904592837997896 1105 1 0.27320057141660925 1105 2 0.18864433054800855 1105 3 0.27287972167697916 1105 4 0.26109428723005834 1105 5 0.21801879340273445 1105 6 0.23144335424971274 1105 7 0.24964506328470293 1105 8 0.19929707636183025 1105 9 0.31818672427505845 1105 10-11 0.35717090180379785 1105 12-13 0.3176136297567922 1105 14-15 0.3848694284911076 1105 16-17 0.3637039763140635 1105 18-19 0.35227573717579475 1105 20-21 0.42718337590186906 1105 22-23 0.46720425271866617 1105 24-25 0.35293478134375533 1105 26-27 0.327887862438196 1105 28-29 0.41750849883683117 1105 30-31 0.4632160462413992 1105 32-33 0.47996252764266956 1105 34-35 0.4642339147292418 1105 36-37 0.44478559316808486 1105 38-39 0.5838684825201454 1105 40-41 0.6139656622365379 1105 42-43 0.34452576440162375 1105 44-45 0.49987414049344636 1105 46-47 0.5853372663715675 1105 48-49 0.6180089034158414 1105 50-51 0.49492301603539346 1105 52-53 0.5812470246756902 1105 54-55 0.6036509726087544 1105 56-57 0.6502047630510148 1105 58-59 0.6944191060658156 1105 60-61 0.7518174934975761 1105 62-63 0.642208219074277 1105 64-65 0.568245938075087 1105 66-67 0.5809814199301755 1105 68-69 0.6738692079991608 1105 70-71 0.4162862562960399 1105 72-73 0.580493134774791 1105 74-75 0.5942840312651256 1105 76-77 0.56967586586903 1105 78-79 0.6150956286332843 1105 80-81 0.7566589055038548 1105 82-83 0.617163969424638 1105 84-85 0.5955997579187553 1105 86-87 0.5526839328223758 1105 88-89 0.4614946458630129 1105 90-91 0.63851880199962 1105 92-93 0.6631329467582106 1105 94-95 0.5932452299690318 1105 96-97 0.5621119671107131 1105 98-99 0.3715218260174282 1105 100-101 0.4794079899911665 1106 1 0.18792269394869265 1106 2 0.28410936745682136 1106 3 0.19891608863990484 1106 4 0.2813325583919237 1106 5 0.16251894704283387 1106 6 0.26149934492340066 1106 7 0.26614202214903315 1106 8 0.17715296429604166 1106 9 0.14197736559931684 1106 10-11 0.2649730626169031 1106 12-13 0.3525157340397129 1106 14-15 0.469427777716227 1106 16-17 0.4761702869110067 1106 18-19 0.4305052285684283 1106 20-21 0.5044857489963235 1106 22-23 0.40042974477747606 1106 24-25 0.3362372693750757 1106 26-27 0.5020573849341616 1106 28-29 0.33861841366626066 1106 30-31 0.21288413671781825 1106 32-33 0.3183384228014745 1106 34-35 0.36970215345076696 1106 36-37 0.34205343828949 1106 38-39 0.4838763404487878 1106 40-41 0.6464725643126741 1106 42-43 0.4314294707637316 1106 44-45 0.6222883541962645 1106 46-47 0.5438591313512404 1106 48-49 0.5173851949895649 1106 50-51 0.5225650392995504 1106 52-53 0.3465720379872579 1106 54-55 0.49775336259188663 1106 56-57 0.5827341833704551 1106 58-59 0.6553376392837436 1106 60-61 0.7155229525296178 1106 62-63 0.7256579553058984 1106 64-65 0.6836366882375202 1106 66-67 0.5513584949511099 1106 68-69 0.5446106798009254 1106 70-71 0.756769131875707 1106 72-73 0.6774373974313264 1106 74-75 0.7003250191592159 1106 76-77 0.6267818963014946 1106 78-79 0.5392979902340507 1106 80-81 0.5107978055345832 1106 82-83 0.46583873562193645 1106 84-85 0.47149198466808073 1106 86-87 0.3914805790115139 1106 88-89 0.498630982097918 1106 90-91 0.5939883894700948 1106 92-93 0.535638347618125 1106 94-95 0.5198351704129713 1106 96-97 0.3862679434759393 1106 98-99 0.0474130558653556 1106 100-101 0.11920229991256193 1107 1 -0.0015663789626430002 1107 2 -0.014933922014087386 1107 3 -0.04933671049254684 1107 4 -0.11401427625786908 1107 5 0.027960313870572406 1107 6 -0.12174985980853847 1107 7 -0.0375125998500323 1107 8 -0.15137746477649472 1107 9 0.12662283104362615 1107 10-11 0.13729365092081736 1107 12-13 0.09563748477386724 1107 14-15 0.11451739328759203 1107 16-17 0.11808994122634431 1107 18-19 0.09278005076505025 1107 20-21 -0.03654194399854305 1107 22-23 -0.00291907540361791 1107 24-25 -0.10691120325470393 1107 26-27 0.056059363798866 1107 28-29 0.03530712080429055 1107 30-31 -0.04354384027357128 1107 32-33 -0.053764529273550465 1107 34-35 -0.030555037850227507 1107 36-37 0.11117723233200394 1107 38-39 0.1941979365181723 1107 40-41 0.08227959889336489 1107 42-43 0.12653028064271865 1107 44-45 0.268706286865978 1107 46-47 0.21312372922837852 1107 48-49 0.14005452903103333 1107 50-51 0.053026194774311364 1107 52-53 0.11006701193757351 1107 54-55 0.19972242185894373 1107 56-57 0.19950706170193655 1107 58-59 0.17448684968228179 1107 60-61 4.568311155495053E-4 1107 62-63 0.2093294048770744 1107 64-65 0.27040562772825893 1107 66-67 0.12184471678617825 1107 68-69 0.12529066593442906 1107 70-71 -0.004462397516711292 1107 72-73 0.03829812580020331 1107 74-75 -0.010038243067576502 1107 76-77 -0.11789289100666167 1107 78-79 -0.11123753099846923 1107 80-81 -0.05858051317485291 1107 82-83 0.015027439982219448 1107 84-85 -0.14195162879886425 1107 86-87 -0.08302042708729829 1107 88-89 -0.036493310827388825 1107 90-91 0.04922171439612555 1107 92-93 0.04423421477974898 1107 94-95 0.006553666075799214 1107 96-97 -0.10440994823871463 1107 98-99 0.05425588889739785 1107 100-101 -0.17188608678493367 1108 1 0.016114544149239407 1108 2 0.09629518668066339 1108 3 0.139298988793378 1108 4 0.12863787011641392 1108 5 0.1219945475790638 1108 6 0.04813898435899944 1108 7 0.015903403838031238 1108 8 0.07765479073721337 1108 9 0.2730780404172961 1108 10-11 0.10127429545121203 1108 12-13 0.09025102247937156 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1147.0 4 1045.0 5 1165.0 6 1319.0 7 1573.0 8 2112.0 9 2571.0 10 3350.0 11 4767.0 12 7022.0 13 11113.0 14 10405.0 15 12271.0 16 16731.0 17 24213.0 18 18680.0 19 10742.0 20 10277.0 21 10655.0 22 11488.0 23 12618.0 24 14341.0 25 16449.0 26 19124.0 27 22538.0 28 26703.0 29 32434.0 30 39787.0 31 49209.0 32 61171.0 33 80535.0 34 110093.0 35 164183.0 36 254067.0 37 417100.0 38 436637.0 39 76786.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36239936523457 20.382772770807204 14.514348343558774 20.740479520399454 2 14.623924648916997 22.732836886667346 39.512614185733455 23.130624278682195 3 20.506533332799894 27.118582190766915 30.725417985268994 21.649466491164198 4 11.697917692933599 17.109605558420075 40.22247883410409 30.96999791454224 5 11.820168748093208 40.06481847326488 34.38509975342973 13.729913025212184 6 28.152069826533204 38.39354792277659 18.06172795080476 15.392654299885447 7 26.560469440390268 35.175540316292455 21.71841573864886 16.545574504668412 8 25.330263586055977 32.73844483577169 20.766480439108488 21.16481113906385 9 26.320677383237364 18.488333733205376 22.956953774792066 32.2340351087652 10-11 24.35750342187516 26.905547748684793 27.630308029992044 21.106640799448 12-13 26.756923729016886 25.58648263369615 27.520123399524955 20.136470237762012 14-15 23.227137254196336 26.42436547005522 28.4833108665674 21.865186409181035 16-17 20.10385269092079 29.975006478922445 28.759693080260917 21.16144774989585 18-19 19.80398105989984 29.495862038974856 31.14204925165811 19.55810764946719 20-21 21.53298189699643 27.224436055648642 30.203438850877156 21.039143196477774 22-23 20.80584688909107 27.367350203461342 30.52824963256981 21.298553274877776 24-25 21.68855734501147 27.79055841540202 30.464460008964085 20.05642423062242 26-27 21.192052152406653 26.619445064516828 32.34675581043933 19.841746972637182 28-29 19.655188531746738 28.667508690399806 31.645678146134692 20.031624631718767 30-31 20.21614639246532 27.52439695291475 31.07258110676171 21.186875547858218 32-33 20.410667169195264 27.93829895236284 31.03247382781581 20.61856005062609 34-35 21.779545114870373 27.19514707748909 30.702730958059654 20.32257684958088 36-37 21.427490004887893 26.386218651693845 31.594806301620526 20.591485041797743 38-39 20.609203939705584 27.979587786351363 31.300206086776967 20.111002187166086 40-41 20.326248644201016 28.089649475381893 31.310293749556838 20.273808130860253 42-43 19.917870754450295 28.409696924739418 29.774883868264407 21.897548452545877 44-45 20.614369714620857 27.387349678428386 29.952202878354196 22.046077728596565 46-47 21.873665255639263 28.435368795506637 29.264238777118496 20.426727171735603 48-49 21.29548089517256 28.096335708511162 29.412271960994442 21.19591143532184 50-51 19.6873825798432 28.538358901422338 30.664135300105556 21.110123218628907 52-53 20.762690515336104 29.601037956553117 28.635649125164154 21.000622402946625 54-55 19.94212387725911 28.19282984715661 29.44125681313897 22.42378946244531 56-57 21.253567878456966 28.21645292179521 28.690265677428954 21.839713522318863 58-59 22.272397241843763 27.419712869942643 28.8687169943493 21.4391728938643 60-61 21.072813822284907 28.187057234901907 30.260665429862804 20.479463512950378 62-63 20.669768183191966 28.116415132072575 30.08110154960547 21.13271513512999 64-65 20.207121402561643 29.216140348414378 29.364116437604125 21.212621811419854 66-67 20.722858160670636 28.164954976794604 28.42763107921071 22.684555783324054 68-69 20.09073105593068 28.213577515907645 29.282177144188115 22.413514283973562 70-71 21.381435441248 28.28174282805895 28.782186608365134 21.554635122327916 72-73 21.31502931832727 27.091031833153135 29.957888198041534 21.63605065047806 74-75 20.071266231568625 29.56538328286938 28.88338038789808 21.479970097663916 76-77 20.568554998949786 28.035786526063138 29.043391689212463 22.35226678577462 78-79 20.61141241018038 27.92636407786697 29.387660387249916 22.07456312470273 80-81 21.192726762426954 28.015496727243566 28.942994897908044 21.84878161242144 82-83 20.83572890439654 27.623870865227634 29.65627586151586 21.88412436885997 84-85 20.395780969749346 27.92663767892179 29.286403197006717 22.391178154322144 86-87 20.420628147724866 28.1410331116533 29.325298215406825 22.11304052521501 88-89 20.47773374138368 27.80990615827985 28.692825972576667 23.019534127759798 90-91 20.569420625608007 27.03778921081927 29.386527063212686 23.00626310036004 92-93 21.383805006217656 27.730003209113885 29.3814934413735 21.504698343294958 94-95 20.88937660501938 26.988443985810772 29.601853655576978 22.520325753592875 96-97 20.275835647926442 26.84217625741451 30.210040862868947 22.6719472317901 98-99 20.773403321075115 27.419915670754435 28.64930560865444 23.157375399516006 100-101 20.49339896479807 26.356944903606816 29.887038119903664 23.26261801169145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2958.0 1 1803.5 2 878.0 3 1112.0 4 917.5 5 924.5 6 1729.0 7 3334.5 8 5039.0 9 5357.0 10 4413.0 11 3752.5 12 3771.0 13 3860.5 14 3909.5 15 4082.0 16 4235.0 17 4208.0 18 4286.0 19 4505.0 20 4868.0 21 5443.5 22 6027.0 23 6821.0 24 7687.0 25 9406.0 26 13031.5 27 16594.0 28 17897.0 29 20000.5 30 24621.5 31 29507.5 32 36534.0 33 44677.0 34 52920.0 35 59281.5 36 66433.5 37 74491.0 38 73911.5 39 70254.0 40 66845.5 41 65319.0 42 65003.0 43 65754.5 44 68177.0 45 68134.5 46 68776.5 47 83117.0 48 124705.0 49 120911.5 50 73496.5 51 51771.0 52 43410.0 53 40931.5 54 39692.5 55 37063.5 56 33341.5 57 29173.0 58 26066.0 59 23360.5 60 20643.5 61 18493.5 62 15982.5 63 12160.0 64 8542.5 65 6503.0 66 4775.0 67 3645.0 68 2888.0 69 2070.0 70 1588.0 71 1280.5 72 1075.0 73 898.0 74 725.5 75 537.0 76 374.0 77 258.5 78 168.0 79 121.5 80 84.0 81 54.0 82 41.0 83 27.0 84 19.5 85 12.5 86 6.5 87 6.5 88 6.5 89 5.5 90 4.0 91 3.0 92 2.5 93 1.5 94 2.5 95 1.5 96 0.5 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2865043275339849 2 0.1124839165980708 3 0.1234725763012571 4 0.12502097835034245 5 0.13156422571905793 6 0.060937112899487736 7 0.06912865922368117 8 0.07317448393258158 9 0.07112659735153322 10-11 0.06660626233726794 12-13 0.0703274221003924 14-15 0.06648139120427718 16-17 0.06720564377562355 18-19 0.07197572105587034 20-21 0.07552206123280773 22-23 0.08411319518257158 24-25 0.09287914871852249 26-27 0.09237966418655946 28-29 0.08613610753702179 30-31 0.0829394065324585 32-33 0.07564693236579849 34-35 0.2414757969775192 36-37 0.3665716980076561 38-39 0.339050100296494 40-41 0.323141517953472 42-43 0.3454435023056206 44-45 0.3583302032302664 46-47 0.36462370833300034 48-49 0.3734396103221475 50-51 0.36749574439178767 52-53 0.3683948165493211 54-55 0.35530832181189015 56-57 0.33243193024798406 58-59 0.31882097675199195 60-61 0.2757154616435838 62-63 0.3451937600396391 64-65 0.3738641721743161 66-67 0.3792336308929185 68-69 0.39052198131528265 70-71 0.39129618233982527 72-73 0.3742387855732884 74-75 0.37815973914919804 76-77 0.36137705887524074 78-79 0.34039870853279414 80-81 0.33984927554763483 82-83 0.3527859249254769 84-85 0.3595289661069776 86-87 0.3532104867776455 88-89 0.3683948165493211 90-91 0.39179566687178835 92-93 0.38680082155215817 94-95 0.3863263112467933 96-97 0.39124623388662905 98-99 0.3805822391292186 100-101 0.35900450734841644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2002064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.533155126026593 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19891357103667 19.459301803488696 2 13.648119678579517 7.7884783193497125 3 5.988659175238117 5.12626023731915 4 3.1948949459936213 3.6464173242157742 5 1.9722198541600047 2.8136827520688477 6 1.2769787549065286 2.1861739743832964 7 0.9086010071418866 1.8147677439110412 8 0.6685144039396163 1.5259860153273817 9 0.49753976941910366 1.2776741479982507 >10 2.9561740409928356 16.077080981004976 >50 0.3649252211480889 7.287633784516885 >100 0.2940360862576652 16.298273050860843 >500 0.02057542467522983 3.982973570249432 >1k 0.007561908213973357 4.278772469132237 >5k 0.0015827249750176794 3.139332028787752 >10k+ 7.034333222300797E-4 3.2971917973857687 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26500 1.3236340097019876 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14690 0.7337427774536678 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12954 0.6470322627048886 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11566 0.5777038096684223 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9910 0.494989171175347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9001 0.44958602721990903 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7426 0.3709172134357343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6708 0.3350542240407899 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6434 0.3213683478650033 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6190 0.30918092528510577 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5876 0.29349711098146714 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5658 0.2826083481846734 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5361 0.26777365758537186 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4928 0.2461459773513734 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4634 0.23146113211166075 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3822 0.190902988116264 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3730 0.18630773042220428 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3456 0.17262185424641768 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3342 0.16692773058203933 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3224 0.16103381310487577 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2975 0.1485966482589967 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2902 0.1449504111756667 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2653 0.13251324632978764 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2517 0.12572025669509065 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2497 0.12472128763116465 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2394 0.1195765969519456 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2218 0.11078566918939654 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2169 0.10833819498277776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2089 0.10434231872707365 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4984535958890424E-4 2 0.0 0.0 0.0 0.0 1.4984535958890424E-4 3 0.0 0.0 0.0 0.0 1.4984535958890424E-4 4 0.0 0.0 0.0 0.0 1.4984535958890424E-4 5 0.0 0.0 0.0 0.0 1.9979381278520568E-4 6 0.0 0.0 0.0 0.0 2.4974226598150706E-4 7 0.0 0.0 0.0 0.0 2.4974226598150706E-4 8 0.0 0.0 0.0 0.0 2.4974226598150706E-4 9 0.0 0.0 0.0 0.0 2.4974226598150706E-4 10-11 0.0 0.0 0.0 0.0 2.4974226598150706E-4 12-13 0.0 0.0 0.0 0.0 5.99381438355617E-4 14-15 0.0 0.0 0.0 7.492267979445213E-5 8.491237043371241E-4 16-17 0.0 0.0 0.0 2.7471649257965777E-4 0.001023943290524179 18-19 0.0 0.0 0.0 3.9958762557041136E-4 0.0010489175171223297 20-21 0.0 0.0 0.0 5.744072117574664E-4 0.0010738917437204804 22-23 0.0 0.0 0.0 0.001198762876711234 0.001098865970318631 24-25 0.0 0.0 0.0 0.0023725515268243173 0.001098865970318631 26-27 0.0 0.0 0.0 0.00459525769405973 0.0012237371033093846 28-29 0.0 0.0 0.0 0.010289381358438092 0.0012487113299075353 30-31 0.0 0.0 0.0 0.027371752351573176 0.001273685556505686 32-33 0.0 0.0 0.0 0.06258541185496568 0.0013985566894964398 34-35 0.0 0.0 0.0 0.11393242174076354 0.0014485051426927412 36-37 0.0 0.0 0.0 0.19335046232288278 0.0014485051426927412 38-39 0.0 0.0 0.0 0.31065440465439664 0.0014485051426927412 40-41 0.0 0.0 0.0 0.43879716132950797 0.0014485051426927412 42-43 0.0 0.0 0.0 0.5729836808413717 0.0014485051426927412 44-45 0.0 0.0 0.0 0.7336928290004715 0.0014984535958890426 46-47 0.0 0.0 0.0 0.9315886005642178 0.0015733762756834947 48-49 0.0 0.0 0.0 1.1343793205412016 0.0015983505022816454 50-51 0.0 0.0 0.0 1.3583481846734171 0.0015983505022816454 52-53 0.0 0.0 0.0 1.5756239560773282 0.0016482989554779468 54-55 0.0 0.0 0.0 1.8241175107289278 0.0017731700884687004 56-57 0.0 0.0 0.0 2.1399166060625436 0.0018480927682631525 58-59 0.0 0.0 0.0 2.4667543095525417 0.0018480927682631525 60-61 0.0 0.0 0.0 2.8337255951857685 0.0018480927682631525 62-63 0.0 0.0 0.0 3.1917561076968566 0.0018480927682631525 64-65 0.0 0.0 0.0 3.5538324449168455 0.0018480927682631525 66-67 0.0 0.0 0.0 3.9762714878245653 0.0018730669948613032 68-69 0.0 0.0 0.0 4.459622669405174 0.001898041221459454 70-71 0.0 0.0 0.0 4.993047175315075 0.001898041221459454 72-73 0.0 0.0 0.0 5.5120116040246465 0.002072860807646509 74-75 0.0 0.0 0.0 6.076753790088628 0.0022726546204317145 76-77 0.0 0.0 0.0 6.6496375740236076 0.002297628847029865 78-79 0.0 0.0 0.0 7.347292594042948 0.0023475773002261664 80-81 0.0 0.0 0.0 8.114950371216905 0.0023725515268243173 82-83 0.0 0.0 0.0 8.831635751904034 0.0024974226598150706 84-85 0.0 0.0 0.0 9.572046647859409 0.0025973195662076735 86-87 0.0 0.0 0.0 10.358759759927754 0.0026972164726002763 88-89 0.0 0.0 0.0 11.186180861351085 0.0028220876055910305 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 570 0.0 31.615814 3 GTACATG 14510 0.0 26.256142 1 TAGGGTC 640 0.0 25.934847 4 TACATGG 14850 0.0 25.132975 2 ACATGGG 14555 0.0 24.567148 3 CATTCCG 655 0.0 23.876745 9 CATGGGG 3525 0.0 23.409155 4 GAGTACT 9700 0.0 23.086294 12-13 TTAGGCA 885 0.0 23.042032 4 GTACACG 165 2.4298988E-6 23.031912 1 GTATAGG 705 0.0 22.9094 1 AGTACTT 10145 0.0 22.517448 12-13 CATGGGA 10170 0.0 22.336245 4 GGATATG 1090 0.0 22.226322 1 TAGGACA 850 0.0 21.759119 4 ATGGGAT 3905 0.0 21.495516 5 TACGAGT 155 3.957017E-5 21.417164 4 GACATAT 875 0.0 21.172909 1 GTATTAC 225 7.152994E-8 21.112587 1 TATAGGG 450 0.0 21.07721 2 >>END_MODULE