##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939326_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2002064 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25407429532722 33.0 31.0 34.0 31.0 34.0 2 32.43575180413813 34.0 31.0 34.0 31.0 34.0 3 32.527298827609904 34.0 31.0 34.0 31.0 34.0 4 36.05184299802604 37.0 35.0 37.0 35.0 37.0 5 35.99888814743185 37.0 35.0 37.0 35.0 37.0 6 36.069297984480016 37.0 35.0 37.0 35.0 37.0 7 36.020115740555745 37.0 35.0 37.0 35.0 37.0 8 36.01462540658041 37.0 35.0 37.0 35.0 37.0 9 37.68648754485371 39.0 37.0 39.0 35.0 39.0 10-11 37.60702554963278 39.0 37.0 39.0 35.0 39.0 12-13 37.55385592068985 39.0 37.0 39.0 35.0 39.0 14-15 38.76805761454179 40.0 38.0 41.0 34.0 41.0 16-17 38.74969256727058 40.0 38.0 41.0 34.5 41.0 18-19 38.92234988491876 40.0 38.0 41.0 35.0 41.0 20-21 38.94133204532922 40.0 38.0 41.0 35.0 41.0 22-23 38.87479396263056 40.0 38.0 41.0 35.0 41.0 24-25 38.78526310847205 40.0 38.0 41.0 34.0 41.0 26-27 38.59271356959618 40.0 38.0 41.0 34.0 41.0 28-29 38.463428242054206 40.0 38.0 41.0 34.0 41.0 30-31 38.27541127556361 40.0 38.0 41.0 34.0 41.0 32-33 38.050506127676236 40.0 37.0 41.0 33.5 41.0 34-35 37.938094886077565 40.0 37.0 41.0 33.0 41.0 36-37 37.81620842290756 40.0 37.0 41.0 33.0 41.0 38-39 37.688737722670204 40.0 37.0 41.0 33.0 41.0 40-41 37.55001063902053 40.0 37.0 41.0 33.0 41.0 42-43 37.33593306707478 40.0 37.0 41.0 32.5 41.0 44-45 37.04628673209248 40.0 36.0 41.0 31.5 41.0 46-47 36.670474570243506 40.0 36.0 41.0 30.5 41.0 48-49 36.29131011795827 40.0 36.0 41.0 30.0 41.0 50-51 36.05211746477635 39.0 35.0 41.0 29.0 41.0 52-53 35.86721578331162 39.0 35.0 41.0 28.5 41.0 54-55 35.6226449304318 39.0 35.0 41.0 28.0 41.0 56-57 35.37857281285713 39.0 35.0 40.0 27.5 41.0 58-59 35.10629205659759 38.0 35.0 40.0 27.0 41.0 60-61 34.73347305580641 38.0 34.0 40.0 26.0 41.0 62-63 34.41139718810188 38.0 34.0 40.0 26.0 41.0 64-65 33.995727908798116 37.0 34.0 39.5 25.5 41.0 66-67 33.59285317552286 36.5 33.0 39.0 24.5 40.5 68-69 33.18317920905625 36.0 33.0 39.0 23.0 40.0 70-71 32.76719625346642 35.5 33.0 38.5 22.5 40.0 72-73 32.271748805233 35.0 32.0 37.5 21.5 39.5 74-75 31.79028692389454 35.0 32.0 37.0 20.0 39.0 76-77 29.78316552318008 33.5 29.5 35.0 14.5 37.5 78-79 30.763013819738028 34.0 31.0 36.0 17.0 38.0 80-81 30.778949873730312 34.0 31.0 36.0 16.5 37.0 82-83 30.471050126269688 34.0 31.0 35.0 12.0 37.0 84-85 30.108610663794963 34.0 31.0 35.0 7.0 36.5 86-87 29.65906534456441 34.0 30.0 35.0 2.0 36.0 88-89 29.16932225942827 34.0 29.5 35.0 2.0 36.0 90-91 28.75592588448721 34.0 29.0 35.0 2.0 35.0 92-93 28.456951426128235 34.0 29.0 35.0 2.0 35.0 94-95 28.103243952241286 34.0 29.0 35.0 2.0 35.0 96-97 27.58452102430292 33.5 28.0 35.0 2.0 35.0 98-99 26.932786863956395 33.0 27.0 35.0 2.0 35.0 100-101 23.741293485123354 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31505812338363626 1101 2 0.2103227135592789 1101 3 0.26119163153023806 1101 4 0.16680928479338775 1101 5 0.22378835546879117 1101 6 0.15040422983709334 1101 7 0.17530750582032084 1101 8 0.20474029288762097 1101 9 0.26795242046208045 1101 10-11 0.22789129940932895 1101 12-13 0.3260489504634734 1101 14-15 0.35171109477663975 1101 16-17 0.349261770236744 1101 18-19 0.3181049715951261 1101 20-21 0.30108697674662466 1101 22-23 0.3089714091354807 1101 24-25 0.32283947499679044 1101 26-27 0.3145441470992054 1101 28-29 0.32537783966662914 1101 30-31 0.30819006724397013 1101 32-33 0.3520723279889495 1101 34-35 0.31417042578063814 1101 36-37 0.31262709129897104 1101 38-39 0.3177717977490957 1101 40-41 0.32185631600761155 1101 42-43 0.41196033812904886 1101 44-45 0.4747591225733103 1101 46-47 0.2911461853637647 1101 48-49 0.43257610654590195 1101 50-51 0.5790498740207042 1101 52-53 0.4900812731625166 1101 54-55 0.3576667367860864 1101 56-57 0.3851696184518403 1101 58-59 0.3282721484865476 1101 60-61 0.44139031034304566 1101 62-63 0.6318506143825715 1101 64-65 0.347678173391337 1101 66-67 0.3154308120280973 1101 68-69 0.3404607089627163 1101 70-71 0.4021870136708827 1101 72-73 0.3508105214183814 1101 74-75 0.2057245132562251 1101 76-77 0.3007147309330662 1101 78-79 0.4260765083473359 1101 80-81 0.5418991973627207 1101 82-83 0.5378651058774757 1101 84-85 0.4907170596391133 1101 86-87 0.5910719338071964 1101 88-89 0.7596017713914911 1101 90-91 0.7786047442750501 1101 92-93 0.7192178885363951 1101 94-95 0.8493038732184601 1101 96-97 0.6405557306036158 1101 98-99 0.5313577272273626 1101 100-101 0.5125943520960696 1102 1 0.21134330506444599 1102 2 0.16394856265052482 1102 3 0.1486116651408338 1102 4 0.1926873263406037 1102 5 0.2514118885356922 1102 6 0.14371317901165526 1102 7 0.15755673263536352 1102 8 0.20747715580440484 1102 9 0.2521589702919087 1102 10-11 0.2849255172568448 1102 12-13 0.27797873538389695 1102 14-15 0.34573674050965053 1102 16-17 0.37932348381232117 1102 18-19 0.3217534160458442 1102 20-21 0.3210076105464523 1102 22-23 0.29917420248679605 1102 24-25 0.3888426383471213 1102 26-27 0.36117990990441484 1102 28-29 0.3455343125310151 1102 30-31 0.3536635812460247 1102 32-33 0.35229298579635326 1102 34-35 0.47036790922248173 1102 36-37 0.44993132369224753 1102 38-39 0.31322448165414585 1102 40-41 0.3397228904098881 1102 42-43 0.3526446950792419 1102 44-45 0.39426209527528755 1102 46-47 0.3506574194940484 1102 48-49 0.26419760603044296 1102 50-51 0.22342009898574844 1102 52-53 0.3670650751141835 1102 54-55 0.23309746082613714 1102 56-57 0.24872577498118176 1102 58-59 0.2549809225470767 1102 60-61 0.1728525831537695 1102 62-63 0.1548883486329018 1102 64-65 0.21786959845241682 1102 66-67 0.1328031129417937 1102 68-69 0.2196899247625268 1102 70-71 0.189334843810272 1102 72-73 0.4176708962189082 1102 74-75 0.19112296829852582 1102 76-77 0.2959633504978214 1102 78-79 0.37139220788554184 1102 80-81 0.31566386669375746 1102 82-83 0.4047523126673802 1102 84-85 0.3069214629097914 1102 86-87 0.5190435766310522 1102 88-89 0.6523776113033684 1102 90-91 0.8440606762630161 1102 92-93 0.7888624352876086 1102 94-95 0.5463643581713598 1102 96-97 0.3796841852222492 1102 98-99 0.29709362984136334 1102 100-101 0.25092773250306877 1103 1 0.2107409676664247 1103 2 0.27914610396271655 1103 3 0.2461255672113296 1103 4 0.19292510913595606 1103 5 0.17047014532055016 1103 6 0.1202651914284445 1103 7 0.08965364111639929 1103 8 0.20051704095600087 1103 9 0.20838432259760964 1103 10-11 0.23679523010157055 1103 12-13 0.24938124272466666 1103 14-15 0.39534871599129673 1103 16-17 0.33526222904553293 1103 18-19 0.26803711764556937 1103 20-21 0.28778808631762587 1103 22-23 0.25388326118899585 1103 24-25 0.2758675438678537 1103 26-27 0.19495346119389723 1103 28-29 0.10554106227318982 1103 30-31 0.18774429719837826 1103 32-33 0.22160324437657408 1103 34-35 0.34331514816677355 1103 36-37 0.3445053190256644 1103 38-39 0.3363038031166141 1103 40-41 0.3524593455275351 1103 42-43 0.3610291083142201 1103 44-45 0.4673540939184804 1103 46-47 0.5163622193487782 1103 48-49 0.4112739740524276 1103 50-51 0.4750201647700436 1103 52-53 0.5358350116940187 1103 54-55 0.45789728128985274 1103 56-57 0.5274851586646321 1103 58-59 0.4927203756466483 1103 60-61 0.5414909511551897 1103 62-63 0.4672845756298116 1103 64-65 0.5205749773459374 1103 66-67 0.5530153357997776 1103 68-69 0.3349501667253634 1103 70-71 0.42579407296915406 1103 72-73 0.6058024853549 1103 74-75 0.7025601837043816 1103 76-77 0.7541632593591743 1103 78-79 0.6953609877582885 1103 80-81 0.5442474829794826 1103 82-83 0.5167305353266407 1103 84-85 0.4426426745741665 1103 86-87 0.507561134526366 1103 88-89 0.6439325531767821 1103 90-91 0.8200948087155737 1103 92-93 0.7239396804334461 1103 94-95 0.4088267120839859 1103 96-97 0.16867970709381197 1103 98-99 0.09082407871635212 1103 100-101 0.10002731004458454 1104 1 0.08283166189387714 1104 2 0.12749256581717106 1104 3 0.13213868480718816 1104 4 0.14449343025120953 1104 5 0.07953920389087443 1104 6 0.0988043384569508 1104 7 0.11833973289586908 1104 8 0.06546261495096672 1104 9 0.1110693874026154 1104 10-11 0.2303748188019341 1104 12-13 0.17972360396475295 1104 14-15 0.24483140491636135 1104 16-17 0.10435503184822181 1104 18-19 0.13343244818931765 1104 20-21 0.19245790674275298 1104 22-23 0.15834732254526074 1104 24-25 0.26183768877321967 1104 26-27 0.25693792547375693 1104 28-29 0.17350346481076429 1104 30-31 0.18177364244752425 1104 32-33 0.029631853991531898 1104 34-35 0.25814181891220755 1104 36-37 0.38363595964651864 1104 38-39 0.352322055584807 1104 40-41 0.31226713378141824 1104 42-43 0.4164620581709144 1104 44-45 0.549417365742606 1104 46-47 0.3744958935502396 1104 48-49 0.31755709124769993 1104 50-51 0.30826063339087284 1104 52-53 0.41332278450687454 1104 54-55 0.42186213108572446 1104 56-57 0.400644707524215 1104 58-59 0.4633314254549106 1104 60-61 0.41059722844558877 1104 62-63 0.39218979899676043 1104 64-65 0.4193524407190026 1104 66-67 0.4359985145406782 1104 68-69 0.5938915818379158 1104 70-71 0.5223751948477116 1104 72-73 0.5371645375557961 1104 74-75 0.4878697145372861 1104 76-77 0.4827706892645267 1104 78-79 0.6343015288500133 1104 80-81 0.6331900270355959 1104 82-83 0.6264820584900157 1104 84-85 0.5835512192202472 1104 86-87 0.522088088964388 1104 88-89 0.8510317873160353 1104 90-91 0.9010746588938368 1104 92-93 0.6973093584660255 1104 94-95 0.6863884124914961 1104 96-97 0.7024187317594048 1104 98-99 0.9546133959968337 1104 100-101 0.9873223248483143 1105 1 0.09340630426378027 1105 2 0.14153170558020634 1105 3 0.10706070531112744 1105 4 0.11888426556115661 1105 5 0.21463761939279635 1105 6 0.16935290274734882 1105 7 0.1415250702975328 1105 8 0.15675948048657773 1105 9 0.2504248323810785 1105 10-11 0.15371526605420627 1105 12-13 0.2274755489705811 1105 14-15 0.2905086141467237 1105 16-17 0.1173039682512993 1105 18-19 0.20925512479660568 1105 20-21 0.12705561179628688 1105 22-23 0.19813007440535557 1105 24-25 0.17277029146745804 1105 26-27 0.19037513711944598 1105 28-29 0.2288490937132721 1105 30-31 0.12709364614600815 1105 32-33 0.18422514350916686 1105 34-35 0.3458878576670301 1105 36-37 0.40123583284138675 1105 38-39 0.45668956780772874 1105 40-41 0.42835562942343586 1105 42-43 0.31320314737417476 1105 44-45 0.3264927147869443 1105 46-47 0.33167368680125975 1105 48-49 0.4858342963266935 1105 50-51 0.47756341272540936 1105 52-53 0.3155398930101825 1105 54-55 0.3520037301512957 1105 56-57 0.4142913552078511 1105 58-59 0.286136819201829 1105 60-61 0.25505195693730087 1105 62-63 0.142639372255978 1105 64-65 0.15510148732177953 1105 66-67 0.1524182697237464 1105 68-69 0.4709976886750056 1105 70-71 0.5704149044790512 1105 72-73 0.4259383205944829 1105 74-75 0.4057449926311385 1105 76-77 0.453306422083088 1105 78-79 0.44029392054201466 1105 80-81 0.36497971543839824 1105 82-83 0.4535727864307333 1105 84-85 0.3358660733471659 1105 86-87 0.4109538623341784 1105 88-89 0.6129471297936782 1105 90-91 0.5791750160993665 1105 92-93 0.5129196930843953 1105 94-95 0.49328372466422366 1105 96-97 0.4915712319150849 1105 98-99 0.6558391789524904 1105 100-101 0.6303550243446772 1106 1 0.12142392478915554 1106 2 0.15174584000003932 1106 3 0.13173577409732218 1106 4 0.11528545152197012 1106 5 0.11324746971685329 1106 6 0.09429795588805234 1106 7 0.12581918201147602 1106 8 0.10441512025097666 1106 9 0.2532484089818041 1106 10-11 0.28064661590561 1106 12-13 0.23037682523892755 1106 14-15 0.20728024866624395 1106 16-17 0.1281933567637097 1106 18-19 0.16017678945772929 1106 20-21 0.13329738735976804 1106 22-23 0.24144719929481084 1106 24-25 0.17495907644403275 1106 26-27 0.26584203882027424 1106 28-29 0.20924502776296805 1106 30-31 0.15863355386812827 1106 32-33 0.20813318463310537 1106 34-35 0.1782333789995434 1106 36-37 0.18651646644845243 1106 38-39 0.2978969561371869 1106 40-41 0.20435335179695358 1106 42-43 0.20294086848103632 1106 44-45 0.2837373731547004 1106 46-47 0.40730507723241516 1106 48-49 0.3952652180969096 1106 50-51 0.21128929064369828 1106 52-53 0.2884564277340189 1106 54-55 0.3640855886569696 1106 56-57 0.3893952088767989 1106 58-59 0.34504665474828755 1106 60-61 0.29557277341127275 1106 62-63 0.3128875537905458 1106 64-65 0.48256629670250817 1106 66-67 0.4343707186999879 1106 68-69 0.39229877400742197 1106 70-71 0.3566100485137156 1106 72-73 0.3994248483517282 1106 74-75 0.36581733248771897 1106 76-77 0.46702730376613744 1106 78-79 0.5367872319200586 1106 80-81 0.4244935559482492 1106 82-83 0.3594412517420835 1106 84-85 0.4598797273778139 1106 86-87 0.4042159817230875 1106 88-89 0.7100371159777978 1106 90-91 0.7059236984302864 1106 92-93 0.718527087512534 1106 94-95 0.9952619875224933 1106 96-97 0.8042176884583903 1106 98-99 0.9732833700666959 1106 100-101 0.9334244837426446 1107 1 -0.030269221183502282 1107 2 -0.05683549956136602 1107 3 -0.0501392252082411 1107 4 -0.05970990964583933 1107 5 -0.013085152884428908 1107 6 0.04336135622427406 1107 7 0.11952287007751039 1107 8 0.0345604184751096 1107 9 -0.0419149279107458 1107 10-11 0.02010980024446951 1107 12-13 0.017499288186606066 1107 14-15 -0.010660976496559726 1107 16-17 -0.009483710516576593 1107 18-19 0.072378279217304 1107 20-21 0.042332665817475856 1107 22-23 -0.06182881622055447 1107 24-25 0.04747355126712449 1107 26-27 0.0848080014585122 1107 28-29 -0.052156638438887626 1107 30-31 -0.03789390524070768 1107 32-33 -0.08661904617296301 1107 34-35 0.015637727390839018 1107 36-37 0.041847262405390495 1107 38-39 0.08776188556581843 1107 40-41 0.1484500072822783 1107 42-43 0.09452733242425637 1107 44-45 0.06227813543426919 1107 46-47 -0.08972634793905598 1107 48-49 0.08632066075261235 1107 50-51 -0.07370750383993396 1107 52-53 0.0438271954246261 1107 54-55 0.11010980391656489 1107 56-57 0.08648752219296796 1107 58-59 -0.06261252941723683 1107 60-61 -0.2544292543417228 1107 62-63 -0.006078131073302018 1107 64-65 0.01286884300471769 1107 66-67 0.10372392660521967 1107 68-69 0.1608679914947615 1107 70-71 0.003076591700406084 1107 72-73 -0.017869218054123337 1107 74-75 -0.023428187844803716 1107 76-77 0.04238638271073114 1107 78-79 0.20627446596497379 1107 80-81 0.18642896562657185 1107 82-83 0.2753983900437227 1107 84-85 0.20599519152214185 1107 86-87 0.1262135461973024 1107 88-89 0.16026633539845037 1107 90-91 0.1279417348765115 1107 92-93 0.10250628332236644 1107 94-95 0.09806920321207713 1107 96-97 0.06765252425484292 1107 98-99 0.10132583288103802 1107 100-101 0.32653648459596596 1108 1 -0.04051998669612544 1108 2 -0.03120726786190886 1108 3 0.035044398976395996 1108 4 -0.011350229815548118 1108 5 -0.038375997598642186 1108 6 0.05676774438325083 1108 7 0.05013080188738428 1108 8 -0.008995714152767675 1108 9 -0.03774055471909321 1108 10-11 0.011446335063986623 1108 12-13 0.02540086502266803 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-1.160741663192134 2316 56-57 -1.0725133834878875 2316 58-59 -0.9858404965470555 2316 60-61 -1.0668345196725966 2316 62-63 -1.087221949832177 2316 64-65 -1.3169571465510472 2316 66-67 -1.0916429971425217 2316 68-69 -1.108085809761377 2316 70-71 -1.0677934482208897 2316 72-73 -1.1973845742441753 2316 74-75 -1.234422308914123 2316 76-77 -1.2585140912034873 2316 78-79 -1.2107578486789663 2316 80-81 -1.2250856246912107 2316 82-83 -1.1503415192405875 2316 84-85 -1.3203486826681683 2316 86-87 -1.4246463381264682 2316 88-89 -1.7185326350199368 2316 90-91 -2.0190974389982266 2316 92-93 -1.7190596220776477 2316 94-95 -1.5491758910849214 2316 96-97 -1.4522829095966294 2316 98-99 -1.4663065722079942 2316 100-101 -0.938107132649936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 16.0 5 64.0 6 131.0 7 327.0 8 668.0 9 1219.0 10 2055.0 11 3399.0 12 5091.0 13 7387.0 14 8390.0 15 11801.0 16 15996.0 17 23093.0 18 20714.0 19 12095.0 20 10260.0 21 10647.0 22 11337.0 23 12373.0 24 14096.0 25 16684.0 26 19936.0 27 24026.0 28 29004.0 29 35742.0 30 43647.0 31 53802.0 32 66183.0 33 85101.0 34 115886.0 35 168729.0 36 264080.0 37 457046.0 38 414756.0 39 36269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35631410121555 21.02579976878983 14.946865739822446 20.671020390172163 2 14.811814207737614 23.15944944816949 38.87932653501586 23.14940980907703 3 20.481912666128554 27.032852096636272 30.97498381670116 21.51025142053401 4 11.847623252803107 17.557880267563874 39.75776998137921 30.836726498253803 5 11.771102222506373 40.37583214123025 34.34585507755995 13.507210558703417 6 28.14375564417521 38.7924162264543 17.71027299826579 15.353555131104699 7 26.629168697903765 35.13808749370649 21.54876167794836 16.683982130441386 8 25.58639484052458 32.979864779547505 20.440055862350054 20.99368451757786 9 26.078786692133715 18.50540242469771 22.782238729631022 32.63357215353755 10-11 24.42159691198683 27.06958918396215 27.520548793644963 20.98826511040606 12-13 26.756786995820313 25.58174963437732 27.442654180885327 20.218809188917035 14-15 23.024047344442508 26.632772380144143 28.422724373366954 21.920455902046395 16-17 20.032906254635872 30.134372201307556 28.716551176768224 21.116170367288344 18-19 19.73872963102079 29.548480967641392 31.078177321004723 19.634612080333095 20-21 21.505331497894172 27.297803666616048 30.240267044410167 20.956597791079606 22-23 20.758202534983898 27.42856871708397 30.518105315314596 21.295123432617537 24-25 21.61321137576874 27.872656323166733 30.521549651921454 19.99258264914307 26-27 21.18291375108577 26.691944330532348 32.378794334353465 19.74634758402842 28-29 19.643303111189255 28.80577244283899 31.626336620607532 19.924587825364224 30-31 20.2233544981579 27.542725906864117 31.105199434183923 21.12872016079406 32-33 20.398923311142898 27.993111109335167 31.028328764714814 20.57963681480712 34-35 21.74027495245338 27.230332932480678 30.76512906581351 20.264263049252428 36-37 21.36025764930728 26.489188466131818 31.659560394487407 20.490993490073492 38-39 20.518894844594545 28.07573373108711 31.3136439552557 20.091727469062647 40-41 20.25107328245457 28.17871330000977 31.268857535882866 20.301355881652796 42-43 19.936256926396005 28.43302731396448 29.819055787302634 21.811659972336876 44-45 20.62428744644532 27.425591614563626 29.959453907696766 21.990667031294294 46-47 21.833780747385006 28.512400099797187 29.285678429797702 20.368140723020108 48-49 21.259260443222594 28.21166056629558 29.378381510281386 21.150697480200435 50-51 19.678616667599037 28.611772650624555 30.577444077711803 21.132166604064608 52-53 20.729482174396026 29.703670811722304 28.552733578946526 21.014113434935147 54-55 19.883680042196453 28.388028554531726 29.220319630141695 22.50797177313013 56-57 21.242438424764856 28.35267921139345 28.59535389245993 21.80952847138177 58-59 22.243095266577942 27.52188318926021 28.73498945206427 21.500032092097577 60-61 20.94817613480838 28.474579572949615 30.044350383514185 20.53289390872782 62-63 20.542879791331487 28.32416845527058 29.83476246823844 21.29818928515949 64-65 20.070637102510208 29.38180297932534 29.085858394137247 21.461701524027205 66-67 20.6798084376923 28.274895308042105 28.200671906592394 22.8446243476732 68-69 20.018860625422537 28.37450229252001 28.996713126440866 22.609923955616594 70-71 21.25829175747922 28.463932337810466 28.538904984781947 21.738870919928363 72-73 21.167055598622223 27.182572585092185 29.654771276043125 21.995600540242467 74-75 19.908254681169034 29.696653054048223 28.414376363592776 21.980715901189974 76-77 20.363399158097835 28.02484905510662 28.794561002613015 22.817190784182532 78-79 20.396650656522468 27.996482629925918 29.069400378809068 22.537466334742547 80-81 20.929422918046512 28.00313980054325 28.648831840354678 22.418605441055565 82-83 20.4340620479665 27.68994897266022 29.366343933061078 22.509645046312205 84-85 19.878410480384243 28.03344448529118 28.912312493506704 23.175832540817876 86-87 19.892182129402944 28.185293340456525 28.894054342426738 23.028470187713793 88-89 19.897309254873086 27.864488113851998 28.215244573437193 24.022958057837727 90-91 19.97325397744332 27.00967337873541 28.91983862163751 24.097234022183763 92-93 20.601813928026278 27.82745706430963 28.907267699733875 22.663461307930216 94-95 20.08527701543932 27.01193044076774 29.121803579157206 23.78098896463574 96-97 19.198836800421965 27.014595936993025 29.77869338842315 24.007873874161863 98-99 19.655990018301114 27.49984515979509 28.143056365830464 24.70110845607333 100-101 19.21525730404846 26.497141066128222 29.386193952970878 24.90140767685244 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1560.0 1 1055.0 2 756.5 3 1007.5 4 855.0 5 804.5 6 1586.5 7 3217.5 8 4733.0 9 4925.5 10 3979.0 11 3267.0 12 3305.5 13 3438.5 14 3486.0 15 3513.0 16 3525.5 17 3642.0 18 3877.5 19 4200.5 20 4616.5 21 5142.5 22 5864.0 23 6687.5 24 7469.0 25 9187.0 26 12654.5 27 16339.0 28 17952.5 29 20216.0 30 24981.0 31 29650.5 32 36651.0 33 44931.5 34 53144.5 35 59417.0 36 66585.5 37 74470.5 38 73732.5 39 69872.5 40 66507.5 41 65055.0 42 64500.0 43 65476.0 44 68136.5 45 68481.0 46 67943.5 47 83544.0 48 125725.5 49 119151.5 50 74542.0 51 56183.0 52 46472.5 53 42206.0 54 40242.0 55 37357.0 56 33544.5 57 29328.0 58 26280.0 59 23930.5 60 21320.5 61 18672.0 62 15735.5 63 11921.5 64 8495.5 65 6336.5 66 4533.5 67 3580.0 68 2780.5 69 1892.0 70 1471.5 71 1208.0 72 999.5 73 854.5 74 691.0 75 515.5 76 348.0 77 210.0 78 146.5 79 105.0 80 56.5 81 27.0 82 15.5 83 8.0 84 4.5 85 2.0 86 2.0 87 2.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28031071933764357 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0027471649257965777 16-17 6.493298915519185E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.492267979445212E-5 26-27 0.0016482989554779466 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004070798935498565 36-37 0.021402912194615157 38-39 0.007766984472024871 40-41 0.018855541081603783 42-43 0.006043762836752471 44-45 0.0010738917437204806 46-47 1.7481958618705495E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.994845319630141E-5 58-59 6.743041181500691E-4 60-61 0.002597319566207674 62-63 0.0018480927682631525 64-65 0.0 66-67 0.0 68-69 4.745103053648635E-4 70-71 2.4974226598150706E-5 72-73 0.0 74-75 0.0 76-77 0.2279397661613215 78-79 0.0 80-81 0.001298659783103837 82-83 0.0 84-85 0.0 86-87 0.10112064349591222 88-89 0.3470168785813041 90-91 0.34968912082730624 92-93 0.0 94-95 2.4974226598150706E-5 96-97 0.0 98-99 0.0 100-101 3.2466494577595924E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2002064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.487624429899604 #Duplication Level Percentage of deduplicated Percentage of total 1 66.76589807286308 18.35235930951818 2 14.22971097652295 7.822819021373655 3 6.190477854650831 5.10484590930758 4 3.3491231717156706 3.6823775965437804 5 2.089740682767722 2.8721003521900554 6 1.3600877816486545 2.243134928019211 7 0.9451022797286761 1.8185031559116633 8 0.7164808379501613 1.5755484947835032 9 0.5047786097449148 1.248764836042357 >10 3.129219023938843 16.450792537920933 >50 0.38352147440039297 7.439745254484796 >100 0.3023462409054596 16.207204344350842 >500 0.022584843218277733 4.155880173938157 >1k 0.008378248290651417 4.290854911306393 >5k 0.001821358324054656 3.4668381448583974 >10k+ 7.285433296218625E-4 3.2682310294504626 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28140 1.405549472943922 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13846 0.6915862829559893 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12435 0.621109015496008 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10859 0.5423902532586371 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9386 0.46881618170048506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9245 0.46177344979980656 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7534 0.3763116463809349 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6816 0.34044865698599047 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6808 0.34004906936042006 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6617 0.3305089147999265 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6343 0.3168230386241399 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5984 0.29889154392666767 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5339 0.26667479161505325 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5175 0.2584832452908598 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4527 0.2261166476196565 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4179 0.20873458590734362 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3838 0.19170216336740484 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3354 0.16752711202039494 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3306 0.1651295862669725 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3081 0.15389118429780468 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3002 0.14994525649529686 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2728 0.13625938031951026 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2484 0.12407195773961271 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2476 0.12367237011404232 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2453 0.12252355569052738 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2407 0.12022592684349752 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2376 0.11867752479441218 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2310 0.11538092688345628 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.10504159707182188 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2074 0.10359309192912913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.994845319630142E-5 3 0.0 0.0 0.0 0.0 4.994845319630142E-5 4 0.0 0.0 0.0 0.0 4.994845319630142E-5 5 0.0 0.0 0.0 0.0 4.994845319630142E-5 6 0.0 0.0 0.0 0.0 9.989690639260284E-5 7 0.0 0.0 0.0 0.0 9.989690639260284E-5 8 0.0 0.0 0.0 0.0 9.989690639260284E-5 9 0.0 0.0 0.0 0.0 9.989690639260284E-5 10-11 0.0 0.0 0.0 0.0 9.989690639260284E-5 12-13 0.0 0.0 0.0 0.0 4.745103053648634E-4 14-15 0.0 0.0 0.0 7.492267979445213E-5 6.243556649537677E-4 16-17 0.0 0.0 0.0 2.7471649257965777E-4 6.992783447482199E-4 18-19 0.0 0.0 0.0 4.495360787667127E-4 7.492267979445213E-4 20-21 0.0 0.0 0.0 6.992783447482198E-4 7.492267979445213E-4 22-23 0.0 0.0 0.0 0.0013236340097019877 8.491237043371241E-4 24-25 0.0 0.0 0.0 0.0024224999800206183 8.491237043371241E-4 26-27 0.0 0.0 0.0 0.004645206147256031 9.240463841315761E-4 28-29 0.0 0.0 0.0 0.010339329811634393 0.0010489175171223297 30-31 0.0 0.0 0.0 0.027421700804769477 0.0010738917437204804 32-33 0.0 0.0 0.0 0.06268530876135828 0.0012487113299075353 34-35 0.0 0.0 0.0 0.114506828952521 0.0013486082363001384 36-37 0.0 0.0 0.0 0.19489886437196813 0.0013486082363001384 38-39 0.0 0.0 0.0 0.31312685308761357 0.0014485051426927412 40-41 0.0 0.0 0.0 0.44264319222562315 0.0014485051426927412 42-43 0.0 0.0 0.0 0.5773042220428518 0.001473479369290892 44-45 0.0 0.0 0.0 0.7392870557584572 0.0015234278224871933 46-47 0.0 0.0 0.0 0.9386812809180926 0.0015983505022816454 48-49 0.0 0.0 0.0 1.1425209184121987 0.0016482989554779468 50-51 0.0 0.0 0.0 1.36816305572649 0.0017481958618705497 52-53 0.0 0.0 0.0 1.5869872291794866 0.0018480927682631525 54-55 0.0 0.0 0.0 1.8367045209343957 0.0018480927682631525 56-57 0.0 0.0 0.0 2.153777301824517 0.0018480927682631525 58-59 0.0 0.0 0.0 2.4817638197380303 0.0018730669948613032 60-61 0.0 0.0 0.0 2.8506581208193147 0.0019479896746557553 62-63 0.0 0.0 0.0 3.20953775703474 0.0020978350342446593 64-65 0.0 0.0 0.0 3.573861774648563 0.0021477834874409608 66-67 0.0 0.0 0.0 3.997424657753199 0.0021727577140391117 68-69 0.0 0.0 0.0 4.482598957875473 0.002197731940637262 70-71 0.0 0.0 0.0 5.017372072021674 0.002197731940637262 72-73 0.0 0.0 0.0 5.538259516179303 0.0022726546204317145 74-75 0.0 0.0 0.0 6.105723892942484 0.0023725515268243173 76-77 0.0 0.0 0.0 6.680430795419127 0.0024474742066187692 78-79 0.0 0.0 0.0 7.380033805113124 0.0024474742066187692 80-81 0.0 0.0 0.0 8.148990242070184 0.0024974226598150706 82-83 0.0 0.0 0.0 8.867398844392586 0.0026972164726002767 84-85 0.0 0.0 0.0 9.609433065076841 0.0026972164726002767 86-87 0.0 0.0 0.0 10.396520790544159 0.0027971133789928796 88-89 0.0 0.0 0.0 11.226039727001734 0.0028970102853854824 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 25 5.2985324E-5 75.93552 9 GGCGCGA 70 2.0653517E-4 33.8964 8 CATATAG 665 0.0 29.257944 3 GTAAGCG 235 0.0 25.241999 94-95 TAGGGTC 625 0.0 25.05622 4 GTACATG 14390 0.0 24.793364 1 TACATGG 14655 0.0 23.962246 2 CATGGGG 3310 0.0 23.655794 4 CCCTATA 465 0.0 23.472343 2 TATAGGG 445 0.0 23.460878 2 ACATGGG 14345 0.0 23.42148 3 GAGTACT 9565 0.0 23.270884 12-13 AGGCTTA 750 0.0 22.77838 6 TACCTGG 910 0.0 21.902288 2 GTATAGG 830 0.0 21.779127 1 AGTACTT 10045 0.0 21.757284 12-13 CATGGGA 9900 0.0 20.995222 4 GTACTTT 10670 0.0 20.860918 14-15 AGGTAAG 205 6.897044E-7 20.833883 2 GTAATAC 255 1.2061719E-8 20.470766 3 >>END_MODULE