FastQCFastQC Report
Fri 27 May 2016
SRR939325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175698
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93010.42749499241163064No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85520.3930692586930723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69890.32123024427103397No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50730.23316655160780583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.2285243632158507No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34570.15889153733652372No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31500.14478112311543237No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23470.10787342728632375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118550.025.5788781
TACATGG119200.025.2591922
ACATGGG119900.024.4789873
GAGTACT80750.021.00764812-13
CATGGGA70100.020.9007764
CATGGGG47150.020.615514
AGTACTT84050.020.46472212-13
GTACTTT85850.019.4601314-15
GTATCAA211000.019.4543111
ATGGGAT29750.019.4439665
ATGGGCG3455.4569682E-1119.2407025
AGAGTAC163150.018.18168810-11
TACCTGG17900.018.012662
TACTGCG1851.5489658E-417.931987
GTGTAGC12300.017.7474081
CATGGGC20250.017.5613614
TACTTTT97300.017.46237414-15
ATGGGGA27100.017.1462355
CAACGCA237650.017.0185745
TCAACGC237450.017.013334
Produced by FastQC (version 0.11.5)