##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939325_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175698 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884157176225745 33.0 31.0 34.0 30.0 34.0 2 31.93466188781715 33.0 31.0 34.0 30.0 34.0 3 31.988478639958302 34.0 31.0 34.0 30.0 34.0 4 35.46796752122767 37.0 35.0 37.0 33.0 37.0 5 35.50881464247336 37.0 35.0 37.0 33.0 37.0 6 35.60408843506774 37.0 35.0 37.0 33.0 37.0 7 35.53964612735775 37.0 35.0 37.0 33.0 37.0 8 35.530348881140675 37.0 35.0 37.0 33.0 37.0 9 37.14578034267623 39.0 37.0 39.0 34.0 39.0 10-11 37.04012390506403 39.0 37.0 39.0 33.0 39.0 12-13 36.93979885995207 39.0 37.0 39.0 33.0 39.0 14-15 38.14910892964005 40.0 38.0 41.0 33.0 41.0 16-17 38.08234001226273 40.0 38.0 41.0 33.0 41.0 18-19 38.02196352618792 40.0 38.0 41.0 33.0 41.0 20-21 38.00344119450402 40.0 38.0 41.0 33.0 41.0 22-23 38.004039163523615 40.0 38.0 41.0 33.0 41.0 24-25 37.917478896427724 40.0 38.0 41.0 33.0 41.0 26-27 37.82424720710319 40.0 38.0 41.0 32.5 41.0 28-29 37.68227070117268 40.0 38.0 41.0 32.0 41.0 30-31 37.49718664998543 40.0 37.0 41.0 32.0 41.0 32-33 37.266332689555256 40.0 37.0 41.0 31.5 41.0 34-35 36.96387596072617 40.0 36.0 41.0 30.5 41.0 36-37 36.72558071019047 40.0 36.0 41.0 30.0 41.0 38-39 36.142627791173226 38.5 35.5 40.5 29.0 41.0 40-41 36.11902433150189 39.0 35.0 40.0 30.0 41.0 42-43 36.28151632257786 39.0 35.0 41.0 30.0 41.0 44-45 36.16622090933576 40.0 35.5 41.0 29.5 41.0 46-47 35.85679997867351 39.0 35.0 41.0 27.5 41.0 48-49 35.583129643911974 39.0 35.0 41.0 26.5 41.0 50-51 34.44444610419277 38.0 34.0 40.0 24.5 40.5 52-53 34.548193729092915 38.5 34.5 39.5 24.5 40.5 54-55 35.03989800054971 39.0 35.0 40.5 25.0 41.0 56-57 34.947342186277695 39.0 35.0 41.0 25.0 41.0 58-59 34.738022464514835 38.5 34.5 40.0 24.0 41.0 60-61 34.42409654281063 38.0 34.0 40.0 23.0 41.0 62-63 34.05561847278437 38.0 34.0 40.0 22.5 41.0 64-65 33.66624366065511 37.0 34.0 40.0 22.0 41.0 66-67 33.287613905974084 36.5 33.0 39.5 20.5 41.0 68-69 32.87420841495465 36.0 33.0 39.0 19.5 41.0 70-71 32.451731352421156 35.5 32.0 39.0 18.0 40.0 72-73 32.00847636023014 35.0 32.0 38.0 15.5 40.0 74-75 31.551242405885375 35.0 31.5 37.0 12.0 39.5 76-77 31.08920493561147 35.0 31.0 37.0 7.5 39.0 78-79 30.689962945224934 35.0 31.0 36.0 7.0 39.0 80-81 30.276080595744446 35.0 30.5 36.0 2.0 37.5 82-83 29.8458269484092 34.0 30.0 35.0 2.0 37.0 84-85 29.42550000045962 34.0 29.5 35.0 2.0 37.0 86-87 29.017773836258524 34.0 29.0 35.0 2.0 36.0 88-89 28.58322156843459 34.0 29.0 35.0 2.0 36.0 90-91 28.16963291780385 34.0 29.0 35.0 2.0 35.5 92-93 27.719216775489983 34.0 27.0 35.0 2.0 35.0 94-95 27.238902412007548 34.0 26.5 35.0 2.0 35.0 96-97 26.630322544764944 33.0 25.0 35.0 2.0 35.0 98-99 25.851699776347637 33.0 24.0 35.0 2.0 35.0 100-101 23.105705617231802 29.5 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27411579201237757 1101 2 0.3080927282413093 1101 3 0.27595645708110794 1101 4 0.319176487931891 1101 5 0.11644582368583656 1101 6 0.22890969536249628 1101 7 0.31961696330375844 1101 8 0.07891263008600191 1101 9 0.04103140662160598 1101 10-11 0.30734421410859625 1101 12-13 0.4026853628222824 1101 14-15 0.4335553739848237 1101 16-17 0.4947175466107794 1101 18-19 0.49654517384744423 1101 20-21 0.5419623509372045 1101 22-23 0.5760810094113182 1101 24-25 0.5794903370224489 1101 26-27 0.5244414940654352 1101 28-29 0.6222975192124593 1101 30-31 0.5104039116416601 1101 32-33 0.4242111163223896 1101 34-35 0.44614134485130563 1101 36-37 0.3379815697517259 1101 38-39 0.7261259212058278 1101 40-41 0.5364819664720955 1101 42-43 0.4716692462535903 1101 44-45 0.4763789524404132 1101 46-47 0.3682048060482259 1101 48-49 0.25567438420966226 1101 50-51 0.17157574979012935 1101 52-53 0.20017408222085464 1101 54-55 0.2416920558163298 1101 56-57 0.29024608255748063 1101 58-59 0.33622418395579956 1101 60-61 0.3035050180719878 1101 62-63 0.48476734805308297 1101 64-65 0.4032534434777091 1101 66-67 0.34491186136020957 1101 68-69 0.39946354980721566 1101 70-71 0.3332649429924359 1101 72-73 0.3420426923105637 1101 74-75 0.26564090146491637 1101 76-77 0.20429616194971345 1101 78-79 0.3413813578647016 1101 80-81 0.2530622781681977 1101 82-83 0.28616196367078217 1101 84-85 0.34626592487797225 1101 86-87 0.272438508236835 1101 88-89 0.04273029659230687 1101 90-91 0.18433326964710162 1101 92-93 0.1806621872442875 1101 94-95 0.13639784633789986 1101 96-97 0.2529411510904538 1101 98-99 0.2885174251233167 1101 100-101 -0.09175576685475306 1102 1 0.4122692884691226 1102 2 0.4480215660241349 1102 3 0.5294081854124997 1102 4 0.37591578123355873 1102 5 0.32644811581809563 1102 6 0.3569337712405627 1102 7 0.45387887549386363 1102 8 0.45957769862712894 1102 9 0.4938755607293004 1102 10-11 0.5893673444821061 1102 12-13 0.4722384447851127 1102 14-15 0.713384663190368 1102 16-17 0.7026164625171205 1102 18-19 0.6421481001362963 1102 20-21 0.6804100574254264 1102 22-23 0.790415935564333 1102 24-25 0.6917245664097038 1102 26-27 0.6662166390746407 1102 28-29 0.6053696980327317 1102 30-31 0.676034741457201 1102 32-33 0.5629957450287932 1102 34-35 0.6479401115568066 1102 36-37 0.6992683048852371 1102 38-39 1.0340151273852385 1102 40-41 0.8468898652023 1102 42-43 0.7895798987260108 1102 44-45 0.8494606181414142 1102 46-47 0.819104759781105 1102 48-49 0.805155126054018 1102 50-51 0.7919140196971952 1102 52-53 0.8961637262399833 1102 54-55 0.900862070481729 1102 56-57 0.9330492382717779 1102 58-59 1.027311477053722 1102 60-61 1.0388259537288604 1102 62-63 1.0208161683477925 1102 64-65 0.9037885396305398 1102 66-67 0.9247587129679928 1102 68-69 0.7963431990685308 1102 70-71 0.8939212886969159 1102 72-73 0.8262769874905089 1102 74-75 0.8738186669330936 1102 76-77 0.813964078677401 1102 78-79 0.6669832548163761 1102 80-81 0.7364615633625142 1102 82-83 0.7321835755066743 1102 84-85 0.8285015019541042 1102 86-87 0.6353224403897215 1102 88-89 0.6004007686229897 1102 90-91 0.7737870471022816 1102 92-93 0.7475299725988336 1102 94-95 0.7125235644862791 1102 96-97 0.7947821153140922 1102 98-99 0.8697026803458776 1102 100-101 0.525155181684756 1103 1 0.29839209055561255 1103 2 0.3530928738762107 1103 3 0.3139687278621679 1103 4 0.2871648953933885 1103 5 0.30667725835610327 1103 6 0.36297286762362546 1103 7 0.45234185480575206 1103 8 0.3978483926095677 1103 9 0.5373707692400416 1103 10-11 0.5161251147148533 1103 12-13 0.5133166589313376 1103 14-15 0.6279410443423004 1103 16-17 0.5386059257775031 1103 18-19 0.5207091254633767 1103 20-21 0.5720125533697242 1103 22-23 0.45371049964754206 1103 24-25 0.5093028944962157 1103 26-27 0.556830957171961 1103 28-29 0.45980849384285705 1103 30-31 0.48255981796928893 1103 32-33 0.3756756833498116 1103 34-35 0.43524291271508986 1103 36-37 0.3022698103572381 1103 38-39 0.771838377567363 1103 40-41 0.5539287681215157 1103 42-43 0.43010426395863277 1103 44-45 0.42574377620137227 1103 46-47 0.4198700891832843 1103 48-49 0.20728536632985595 1103 50-51 0.24403067578679583 1103 52-53 0.2844194541515961 1103 54-55 0.2709698731235832 1103 56-57 0.28924094164529635 1103 58-59 0.35744553028808923 1103 60-61 0.3731442492029018 1103 62-63 0.26853332108149175 1103 64-65 0.15797773579609498 1103 66-67 0.3238821809664145 1103 68-69 0.1946922902317212 1103 70-71 0.08505157121148699 1103 72-73 0.14430455843464074 1103 74-75 0.27173520214690683 1103 76-77 0.2057150620316861 1103 78-79 0.1761507553939019 1103 80-81 0.13824111783248938 1103 82-83 0.22590708178069008 1103 84-85 0.26533946842489087 1103 86-87 0.05348136858960473 1103 88-89 -0.1428595455810644 1103 90-91 -0.046842638594583264 1103 92-93 0.13558376284752427 1103 94-95 0.10412645152999289 1103 96-97 0.2041638601493645 1103 98-99 0.3690311626559257 1103 100-101 -0.04969016457119935 1104 1 0.19929650592548498 1104 2 0.30777394310249306 1104 3 0.28315085805484586 1104 4 0.1454572175772526 1104 5 0.15612836336838143 1104 6 0.16487528312777044 1104 7 0.28936653066678275 1104 8 0.3694738989336557 1104 9 0.44275657243127853 1104 10-11 0.4402833095868104 1104 12-13 0.43970842713407876 1104 14-15 0.4825075230934672 1104 16-17 0.45979763620182723 1104 18-19 0.4958651678857535 1104 20-21 0.5912777909020974 1104 22-23 0.6619765265470576 1104 24-25 0.5935370659557151 1104 26-27 0.5092314063788237 1104 28-29 0.5082345405172433 1104 30-31 0.4531141856312715 1104 32-33 0.4696286834535712 1104 34-35 0.43199007923289656 1104 36-37 0.40829956827786873 1104 38-39 0.8113497125133406 1104 40-41 0.5848964244131594 1104 42-43 0.5553850182518332 1104 44-45 0.6323311263894524 1104 46-47 0.6655407766040611 1104 48-49 0.4970728780533804 1104 50-51 0.4058031305355101 1104 52-53 0.5621406346216773 1104 54-55 0.6082163041872093 1104 56-57 0.5016249524848533 1104 58-59 0.477038721386144 1104 60-61 0.6109355063514528 1104 62-63 0.6874795057730694 1104 64-65 0.4919202177861166 1104 66-67 0.5346790654075377 1104 68-69 0.5072921055035309 1104 70-71 0.6078165737647225 1104 72-73 0.49094278007998327 1104 74-75 0.5931263905310011 1104 76-77 0.5398164855373331 1104 78-79 0.4085291002863052 1104 80-81 0.5877007675406496 1104 82-83 0.5440311955227983 1104 84-85 0.6017075250965682 1104 86-87 0.6588262793077035 1104 88-89 0.5604653074806834 1104 90-91 0.7285145093486847 1104 92-93 0.7392746645774224 1104 94-95 0.6720878091601001 1104 96-97 0.8275182432731825 1104 98-99 0.7930042224504881 1104 100-101 0.67048020038904 1105 1 0.41185893083465785 1105 2 0.5372146159163513 1105 3 0.47370714541932557 1105 4 0.433184540748897 1105 5 0.39122599954514925 1105 6 0.3749380578576904 1105 7 0.41543734403620647 1105 8 0.455560200918228 1105 9 0.6669173645219502 1105 10-11 0.6327790514239311 1105 12-13 0.5355157651670766 1105 14-15 0.7016083390202041 1105 16-17 0.7333884617305344 1105 18-19 0.721898224740066 1105 20-21 0.7315580817599567 1105 22-23 0.7135913714920221 1105 24-25 0.7797564204520029 1105 26-27 0.6972776695694805 1105 28-29 0.7782734791852235 1105 30-31 0.8185762626497421 1105 32-33 0.8970572838328152 1105 34-35 0.5984117507488094 1105 36-37 0.6964533108773452 1105 38-39 1.1238140669854957 1105 40-41 1.0775650259504346 1105 42-43 0.9343594644494075 1105 44-45 0.9204888497077874 1105 46-47 1.0703538275717364 1105 48-49 1.0221985722666247 1105 50-51 0.995281969999148 1105 52-53 0.8359177095412136 1105 54-55 1.0968553326023098 1105 56-57 1.0507506418729875 1105 58-59 1.1078716807731652 1105 60-61 1.1690677530691715 1105 62-63 0.9371691303309575 1105 64-65 1.0283570642847764 1105 66-67 1.0565618006680282 1105 68-69 0.9317418012801113 1105 70-71 0.8903503857493362 1105 72-73 0.8742084411929412 1105 74-75 0.9785720380371465 1105 76-77 0.8585312923639066 1105 78-79 0.7202079262396275 1105 80-81 0.6788290721404309 1105 82-83 0.6527938433522209 1105 84-85 0.777035753459554 1105 86-87 0.7779441037604151 1105 88-89 0.7503034972910108 1105 90-91 0.753390986436635 1105 92-93 0.949335026750461 1105 94-95 1.0143647320885592 1105 96-97 0.9639456808000233 1105 98-99 0.9029569845868544 1105 100-101 0.7051903799315191 1106 1 0.14487876556239243 1106 2 0.08704341414503958 1106 3 0.1598392878235515 1106 4 0.12215782139070086 1106 5 0.05545117014711565 1106 6 0.13200680019588162 1106 7 0.11697319015683405 1106 8 0.10492663440317074 1106 9 0.08533380811790892 1106 10-11 0.19222828098740052 1106 12-13 0.17935701422594974 1106 14-15 0.1506784112139954 1106 16-17 0.15069852030588038 1106 18-19 0.21019629519270921 1106 20-21 0.19872595774977952 1106 22-23 0.1837713018688305 1106 24-25 0.08149734310215706 1106 26-27 6.386396785771353E-4 1106 28-29 0.1144434950543669 1106 30-31 -0.0019369884930355852 1106 32-33 0.04059737315250089 1106 34-35 -0.09264954028505912 1106 36-37 -0.11441688767557423 1106 38-39 0.4696169673045958 1106 40-41 0.3729238984522425 1106 42-43 0.304784344174017 1106 44-45 0.13547097548882192 1106 46-47 0.0186834535327165 1106 48-49 -0.008612376877820793 1106 50-51 0.09634322675839968 1106 52-53 0.061659783818910796 1106 54-55 0.028709487201446393 1106 56-57 0.12599887587273173 1106 58-59 0.29188587960942414 1106 60-61 0.37048637995899014 1106 62-63 0.07702149807945347 1106 64-65 0.23690561372535512 1106 66-67 0.12503839623769153 1106 68-69 0.22507629995082112 1106 70-71 0.1481804818439727 1106 72-73 0.14416787145720633 1106 74-75 0.24374604461558746 1106 76-77 0.27121907448694316 1106 78-79 0.38301812591219075 1106 80-81 0.4709528766699371 1106 82-83 0.4350837998577184 1106 84-85 0.49338440869448874 1106 86-87 0.5165593859034487 1106 88-89 0.5917944207980348 1106 90-91 0.5259354092118969 1106 92-93 0.5204164941158709 1106 94-95 0.5045060920829805 1106 96-97 0.7479299464603848 1106 98-99 0.818310418847549 1106 100-101 0.6677010193475716 1107 1 0.2192907835176463 1107 2 0.16066191312446065 1107 3 0.2543486872317473 1107 4 0.21705469233404528 1107 5 0.19636427424649128 1107 6 0.17016389553617017 1107 7 0.13587493891443359 1107 8 0.11158620580239642 1107 9 0.3331143152182747 1107 10-11 0.330517374526913 1107 12-13 0.3444497189215028 1107 14-15 0.40451848796526946 1107 16-17 0.3435363067572723 1107 18-19 0.3691156801335538 1107 20-21 0.31157480232845103 1107 22-23 0.3787856380970922 1107 24-25 0.4220286577080685 1107 26-27 0.5197283328920363 1107 28-29 0.3486038204168196 1107 30-31 0.30032774220003944 1107 32-33 0.48380733333450365 1107 34-35 0.4195429192085314 1107 36-37 0.46424256571201283 1107 38-39 0.9707284620749306 1107 40-41 0.763509848074392 1107 42-43 0.5668815201992956 1107 44-45 0.6333823880880871 1107 46-47 0.6481935741231268 1107 48-49 0.7239234309118174 1107 50-51 0.5176974321248622 1107 52-53 0.661674535273292 1107 54-55 0.7254227538946267 1107 56-57 0.5334300029712651 1107 58-59 0.5573425627831483 1107 60-61 0.6428351611610452 1107 62-63 0.5607687827191441 1107 64-65 0.5138624214669107 1107 66-67 0.5137890463943791 1107 68-69 0.6246273093873853 1107 70-71 0.736651509987567 1107 72-73 0.6681389848314225 1107 74-75 0.6737071226300237 1107 76-77 0.6700321833941985 1107 78-79 0.8330574631238825 1107 80-81 0.6174249844014454 1107 82-83 0.5465038294917548 1107 84-85 0.5265935384242404 1107 86-87 0.5109578762286802 1107 88-89 0.6230753715347355 1107 90-91 0.6182409413351024 1107 92-93 0.6426836506036686 1107 94-95 0.6825048988542299 1107 96-97 0.5965106380085672 1107 98-99 0.6771231562366928 1107 100-101 0.4036646280559566 1108 1 0.056920024816612624 1108 2 0.13257832494912591 1108 3 0.17921294486140482 1108 4 0.1977647133455136 1108 5 0.3052213338899179 1108 6 0.3077036820929919 1108 7 0.31452542051380306 1108 8 0.30643215641505606 1108 9 0.27750155023461076 1108 10-11 0.37732300809532404 1108 12-13 0.36605459940580687 1108 14-15 0.3994304894631142 1108 16-17 0.3935134229860253 1108 18-19 0.44964419123218136 1108 20-21 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64-65 -1.176594067019444 2316 66-67 -1.2718989234136089 2316 68-69 -1.5235619120909334 2316 70-71 -1.2327736366736701 2316 72-73 -1.1499635347377932 2316 74-75 -1.1718839765264413 2316 76-77 -1.138471060590014 2316 78-79 -1.0668912615535326 2316 80-81 -1.1520484163091567 2316 82-83 -0.8932509467095926 2316 84-85 -0.890735276235322 2316 86-87 -0.7061897483599964 2316 88-89 -0.8866510564916332 2316 90-91 -0.9606402725606848 2316 92-93 -1.0814892111117942 2316 94-95 -0.9865105631588733 2316 96-97 -1.14795654751142 2316 98-99 -1.206566025533462 2316 100-101 -0.4930131799153692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8811.0 3 2127.0 4 2130.0 5 2355.0 6 2697.0 7 3272.0 8 4119.0 9 4949.0 10 5653.0 11 7123.0 12 9131.0 13 12219.0 14 12273.0 15 13266.0 16 16305.0 17 21175.0 18 18767.0 19 13351.0 20 13035.0 21 13925.0 22 14874.0 23 16201.0 24 18341.0 25 20781.0 26 24135.0 27 27905.0 28 32903.0 29 39194.0 30 47118.0 31 56919.0 32 71773.0 33 92727.0 34 127908.0 35 183678.0 36 284880.0 37 448554.0 38 418680.0 39 62426.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42818270532546 18.792342303513504 14.312015499409275 22.467459491751764 2 15.064540147134867 20.574292885595895 40.62467624021008 23.73649072705916 3 19.873024819006492 25.042373408148126 29.63168633041451 25.452915442430875 4 12.554288403950862 16.336860294923625 37.59230755768272 33.51654374344279 5 13.383835941145392 38.048215158428675 33.84982890191836 14.718119998507584 6 31.204199370720175 35.89030027451619 17.952190193799414 14.95331016096422 7 28.407855128103055 33.16421978195949 20.632114055922525 17.79581103401493 8 26.33056882781069 34.395103515918954 19.808888731375948 19.465438924894404 9 26.730989368895237 17.416839924016852 20.772286679428717 35.07988402765919 10-11 25.16030173198356 26.597212746084587 27.084888985944993 21.157596535986862 12-13 26.388302482678363 24.13071974226402 27.621600067922138 21.859377707135483 14-15 22.949214098115366 25.389890316848373 26.82546173447256 24.835433850563696 16-17 21.834437284165915 28.818316304345327 27.197130571423973 22.15011584006479 18-19 21.630896625620814 27.563348286644224 29.63132835615836 21.174426731576602 20-21 22.90652572746588 26.247217548035422 29.287945508829033 21.558311215669665 22-23 22.217070887834783 26.331365441748922 29.331590576035303 22.119973094380992 24-25 22.313221053940392 26.420993613912852 29.551246996627935 21.714538335518824 26-27 21.992726773801376 26.43912339138516 29.848635216881142 21.719514617932322 28-29 21.426520992177068 27.18972712621457 29.78909181650281 21.594660065105547 30-31 21.778235069353332 26.540965192131516 29.563416980862804 22.117382757652347 32-33 21.719234725213607 26.67566153984985 29.549631718184994 22.05547201675155 34-35 22.450526247813308 26.654558411950504 29.17632732417949 21.7185880160567 36-37 22.391353140739607 26.280989674495846 29.520121293962365 21.807535890802182 38-39 21.870065176317695 26.748490049271773 29.596488534992883 21.78495623941765 40-41 22.00209715462326 26.770434966856598 29.48771124170434 21.739756636815795 42-43 21.784530766840415 26.850657760773718 28.923327963386473 22.441483508999394 44-45 22.016227937319456 26.804148227228065 28.837442649959733 22.34218118549275 46-47 22.315791904044517 27.198254515229703 28.378026747937916 22.107926832787868 48-49 22.354673287372627 26.9763749418102 28.474000243842966 22.194951526974204 50-51 21.941534834695812 27.347617326894415 28.621608859939666 22.089238978470107 52-53 22.282779745005698 27.610643581789024 27.998318972633662 22.108257700571617 54-55 21.738135689676707 27.16494703745183 28.41339530786061 22.68352196501085 56-57 22.227958432502206 27.41318738075792 28.093509099842446 22.26534508689743 58-59 22.591370022487002 26.91667349314602 28.199413191988675 22.2925432923783 60-61 22.231163618557726 27.073310300972935 28.874105985299973 21.821420095169366 62-63 21.955569553582745 27.16187356102346 28.70912763991799 22.173429245475802 64-65 21.785270461169162 27.40537565039015 28.378115026523588 22.431238861917098 66-67 21.88308803289803 27.302728201452403 28.071466574544303 22.742717191105267 68-69 21.73669444498332 27.247230713377103 28.34455274323579 22.671522098403788 70-71 22.078438526927883 27.29245520487175 28.137229318264218 22.491876949936145 72-73 22.098153618719344 26.957480743337094 28.463953665318193 22.48041197262537 74-75 21.71302987777829 27.771810930561063 27.95992249230962 22.55523669935103 76-77 21.90930872443865 27.16919677095988 28.02694806948817 22.894546435113295 78-79 21.976087506016214 27.229357035764863 28.144467566703486 22.65008789151544 80-81 22.169718553043914 27.461160467791768 27.89611429246773 22.473006686696596 82-83 22.144321633469843 27.287053319232275 28.14128948983849 22.4273355574594 84-85 21.898602234070673 27.330762278211868 28.01100297699094 22.75963251072652 86-87 21.99164347976252 27.227974441921305 28.08045678739864 22.699925290917538 88-89 21.979279415730506 27.197475406081338 27.80906788771209 23.014177290476066 90-91 21.907766374859623 27.07510415384541 28.059730651378068 22.9573988199169 92-93 22.177489195484483 27.02084970118708 28.17365467211869 22.628006431209748 94-95 21.973002314199523 26.91937574577568 28.119775817550273 22.987846122474522 96-97 21.780774339675624 26.85509258489489 28.374965591266736 22.989167484162746 98-99 21.8934211641431 27.03831729149439 27.812671532523087 23.255590011839423 100-101 21.966008826105767 26.28158389672062 28.503209933778813 23.249197343394805 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3070.0 1 1823.0 2 742.0 3 890.5 4 728.0 5 680.5 6 1098.0 7 2062.0 8 3243.5 9 3747.5 10 3364.5 11 2976.0 12 3124.0 13 3334.0 14 3544.0 15 3752.0 16 3903.5 17 4034.5 18 4086.5 19 4241.5 20 4575.0 21 5178.0 22 6076.0 23 7248.5 24 8502.5 25 10201.5 26 12822.0 27 15792.5 28 18155.0 29 20949.5 30 24630.0 31 28484.5 32 33054.0 33 37619.0 34 41761.0 35 45075.0 36 49071.5 37 54004.5 38 57207.5 39 59006.5 40 61006.0 41 65151.0 42 69467.5 43 72484.0 44 75698.5 45 79700.0 46 81959.0 47 87469.0 48 104036.0 49 103946.5 50 87601.5 51 79307.5 52 74774.5 53 72679.5 54 69891.5 55 63716.5 56 55885.0 57 48978.5 58 43106.5 59 36900.5 60 30815.0 61 25906.5 62 21631.0 63 16849.5 64 12656.0 65 9622.5 66 7199.5 67 5997.0 68 4706.0 69 3208.5 70 2436.0 71 1919.5 72 1604.0 73 1337.0 74 1040.0 75 876.5 76 637.5 77 366.0 78 246.5 79 170.5 80 110.5 81 69.0 82 48.5 83 37.0 84 27.0 85 24.0 86 14.0 87 8.0 88 7.5 89 5.5 90 6.0 91 7.5 92 5.5 93 4.5 94 4.5 95 3.5 96 4.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2907113027635269 2 0.18150496989931508 3 0.18086149824102427 4 0.20333704401989616 5 0.21698783562792262 6 0.11122867236169727 7 0.12864836939685564 8 0.1395873875877994 9 0.1429885949244794 10-11 0.1306477277636878 12-13 0.1204900680149543 14-15 0.13510606711041698 16-17 0.11228580437174646 18-19 0.14002402907021103 20-21 0.16560202748727076 22-23 0.16826783864304695 24-25 0.1921452333917667 26-27 0.1727951213817359 28-29 0.16943987630636237 30-31 0.16031636743702482 32-33 0.17097961206012968 34-35 0.35646031756245583 36-37 0.47708827236132956 38-39 0.4435588027382477 40-41 0.43340114298951415 42-43 0.456910839647782 44-45 0.461920726130189 46-47 0.4647014429392315 48-49 0.4766286497482647 50-51 0.469757291682945 52-53 0.4759621969593206 54-55 0.45822076409501683 56-57 0.4205317098236979 58-59 0.40862748414531797 60-61 0.36211367570315367 62-63 0.47403178198444823 64-65 0.47189453683369653 66-67 0.48085717778846143 68-69 0.49179619597940527 70-71 0.48549936618041656 72-73 0.47839819680856444 74-75 0.48230498901961577 76-77 0.45856548105481554 78-79 0.44470785927090983 80-81 0.441513482110109 82-83 0.45518725484878875 84-85 0.4559686132909991 86-87 0.4579679716578312 88-89 0.4652300089442561 90-91 0.4831323097231326 92-93 0.4886247999492577 94-95 0.486579479321119 96-97 0.48490185678343223 98-99 0.4814317060547925 100-101 0.4631617071854641 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175698.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.09420443370855 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0890893293274 42.47162047118383 2 13.070249010027544 14.401899419158685 3 4.343041513902763 7.178292510931258 4 1.866010751344826 4.112255112403598 5 0.9762533102473553 2.6892949726926245 6 0.5924274312633855 1.9583590806097069 7 0.36542584376965503 1.409299229940407 8 0.2708419008761137 1.1937455244866269 9 0.2010401323442136 0.9968531535676733 >10 1.0804299563526976 10.999983257405098 >50 0.09228235244235576 3.529821976932105 >100 0.04780446395559965 4.805432491054304 >500 0.003179543566484562 1.1769416316301164 >1k 0.001589771783242246 1.6971635701732646 >5k 3.346887964720518E-4 1.3790375978306502 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9301 0.42749499241163064 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8552 0.3930692586930723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6989 0.32123024427103397 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5073 0.23316655160780583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4972 0.2285243632158507 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3457 0.15889153733652372 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3150 0.14478112311543237 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2347 0.10787342728632375 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 2.2981130653243235E-5 0.0 0.0 0.0 0.0 18-19 4.596226130648647E-5 0.0 0.0 6.894339195972971E-5 0.0 20-21 4.596226130648647E-5 0.0 0.0 1.3788678391945942E-4 0.0 22-23 4.596226130648647E-5 4.596226130648647E-5 0.0 2.068301758791891E-4 0.0 24-25 2.2981130653243234E-4 4.596226130648647E-5 0.0 2.9875469849216204E-4 0.0 26-27 2.2981130653243234E-4 4.596226130648647E-5 0.0 9.192452261297294E-4 0.0 28-29 2.7577356783891883E-4 4.596226130648647E-5 0.0 0.0029875469849216206 0.0 30-31 2.7577356783891883E-4 4.596226130648647E-5 0.0 0.009054565477377834 0.0 32-33 2.7577356783891883E-4 4.596226130648647E-5 0.0 0.020568111934652696 0.0 34-35 3.217358291454053E-4 4.596226130648647E-5 0.0 0.037252412788907285 0.0 36-37 3.217358291454053E-4 4.596226130648647E-5 0.0 0.06519746766325106 0.0 38-39 3.217358291454053E-4 4.596226130648647E-5 0.0 0.10966595547727673 0.0 40-41 3.217358291454053E-4 4.596226130648647E-5 0.0 0.15725987706014347 0.0 42-43 3.4471695979864856E-4 4.596226130648647E-5 0.0 0.20781836449727858 0.0 44-45 3.6769809045189176E-4 4.596226130648647E-5 0.0 0.2674084362811383 0.0 46-47 4.136603517583782E-4 4.596226130648647E-5 0.0 0.34106296002478287 0.0 48-49 4.136603517583782E-4 4.596226130648647E-5 0.0 0.42282982288902227 0.0 50-51 4.136603517583782E-4 4.596226130648647E-5 0.0 0.5141108738437044 0.0 52-53 4.136603517583782E-4 4.596226130648647E-5 0.0 0.6091838113561716 0.0 54-55 4.136603517583782E-4 4.596226130648647E-5 0.0 0.7145293142706386 0.0 56-57 4.136603517583782E-4 4.596226130648647E-5 0.0 0.8441658722855838 0.0 58-59 4.136603517583782E-4 4.596226130648647E-5 0.0 0.9874762030392086 0.0 60-61 4.136603517583782E-4 4.596226130648647E-5 0.0 1.1475397780390477 0.0 62-63 4.136603517583782E-4 4.596226130648647E-5 0.0 1.3136014281393833 0.0 64-65 4.136603517583782E-4 4.596226130648647E-5 0.0 1.4865114551743854 0.0 66-67 4.136603517583782E-4 4.596226130648647E-5 0.0 1.689228008666644 0.0 68-69 4.136603517583782E-4 4.596226130648647E-5 0.0 1.924003239420177 0.0 70-71 4.136603517583782E-4 4.596226130648647E-5 0.0 2.183644053540519 0.0 72-73 4.136603517583782E-4 4.596226130648647E-5 0.0 2.4412625281633753 0.0 74-75 4.136603517583782E-4 4.596226130648647E-5 0.0 2.7169671526103345 0.0 76-77 4.136603517583782E-4 4.596226130648647E-5 0.0 3.013860379519584 0.0 78-79 4.136603517583782E-4 4.596226130648647E-5 0.0 3.361059301428783 0.0 80-81 5.975093969843241E-4 4.596226130648647E-5 0.0 3.748475201981157 0.0 82-83 5.975093969843241E-4 4.596226130648647E-5 0.0 4.141659366327496 0.0 84-85 6.204905276375673E-4 4.596226130648647E-5 0.0 4.546471982784375 0.0 86-87 6.434716582908106E-4 4.596226130648647E-5 0.0 4.971278182909577 0.0 88-89 6.434716582908106E-4 4.596226130648647E-5 0.0 5.438323701175439 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11855 0.0 25.578878 1 TACATGG 11920 0.0 25.259192 2 ACATGGG 11990 0.0 24.478987 3 GAGTACT 8075 0.0 21.007648 12-13 CATGGGA 7010 0.0 20.900776 4 CATGGGG 4715 0.0 20.61551 4 AGTACTT 8405 0.0 20.464722 12-13 GTACTTT 8585 0.0 19.46013 14-15 GTATCAA 21100 0.0 19.454311 1 ATGGGAT 2975 0.0 19.443966 5 ATGGGCG 345 5.4569682E-11 19.240702 5 AGAGTAC 16315 0.0 18.181688 10-11 TACCTGG 1790 0.0 18.01266 2 TACTGCG 185 1.5489658E-4 17.93198 7 GTGTAGC 1230 0.0 17.747408 1 CATGGGC 2025 0.0 17.561361 4 TACTTTT 9730 0.0 17.462374 14-15 ATGGGGA 2710 0.0 17.146235 5 CAACGCA 23765 0.0 17.018574 5 TCAACGC 23745 0.0 17.01333 4 >>END_MODULE