FastQCFastQC Report
Fri 27 May 2016
SRR939325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175698
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96530.44367370839151393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95190.43751476537644474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78290.35983854376848257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54810.2519191542208523No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45010.2068761381404956No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30160.1386221801003632No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28800.13237131256268103No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26020.1195938039194778No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22780.10470203125617616No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGA909.0005994E-426.3634824
GTACATG116100.023.8586271
TACCTGG18300.023.5974752
TACATGG115400.023.2747972
ACATGGG116950.021.951913
GAGTACT90050.021.68659212-13
GTACTTT95200.020.43864614-15
GTACCTG21650.020.2248171
CATGGGG48350.020.2183654
AGTACTT94250.020.11595312-13
CCTATAC3759.094947E-1218.9817073
GTATAGG5550.018.8661981
GTATAGC8900.018.7168481
GTATCAA225200.017.5414071
ACTTTTT111950.017.44339416-17
TACTTTT108950.017.35826314-15
AGAGTAC175950.017.23502210-11
CATGGGA68800.017.1745824
ATGGGGG26400.017.0763455
TAGACCG1700.001799814916.7485645
Produced by FastQC (version 0.11.5)